Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/23/e02d16dcfa2dd1859f38d494e7fc10/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5c/85e3de4a32259212df1aa8028891a6/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5c/85e3de4a32259212df1aa8028891a6/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/06/1e3028c9ff4644ff6a4f19ee33bc65/rna_s1200_S21.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5c/189b9b926a44769e082247f36a92b5/rna_s1200_S21.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: rna_s1200_S21.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (586985) splicing reads. 95587 splicing junctions. 90961 known splicing junctions. 4626 novel splicing junctions.
sampling 10% (1173971) splicing reads. 114522 splicing junctions. 106053 known splicing junctions. 8469 novel splicing junctions.
sampling 15% (1760957) splicing reads. 125441 splicing junctions. 113686 known splicing junctions. 11755 novel splicing junctions.
sampling 20% (2347942) splicing reads. 133241 splicing junctions. 118550 known splicing junctions. 14691 novel splicing junctions.
sampling 25% (2934928) splicing reads. 139763 splicing junctions. 122339 known splicing junctions. 17424 novel splicing junctions.
sampling 30% (3521914) splicing reads. 145099 splicing junctions. 125174 known splicing junctions. 19925 novel splicing junctions.
sampling 35% (4108899) splicing reads. 149611 splicing junctions. 127507 known splicing junctions. 22104 novel splicing junctions.
sampling 40% (4695885) splicing reads. 153618 splicing junctions. 129472 known splicing junctions. 24146 novel splicing junctions.
sampling 45% (5282871) splicing reads. 157123 splicing junctions. 131121 known splicing junctions. 26002 novel splicing junctions.
sampling 50% (5869857) splicing reads. 160332 splicing junctions. 132596 known splicing junctions. 27736 novel splicing junctions.
sampling 55% (6456842) splicing reads. 163145 splicing junctions. 133832 known splicing junctions. 29313 novel splicing junctions.
sampling 60% (7043828) splicing reads. 165695 splicing junctions. 134918 known splicing junctions. 30777 novel splicing junctions.
sampling 65% (7630814) splicing reads. 168146 splicing junctions. 135965 known splicing junctions. 32181 novel splicing junctions.
sampling 70% (8217799) splicing reads. 170383 splicing junctions. 136875 known splicing junctions. 33508 novel splicing junctions.
sampling 75% (8804785) splicing reads. 172382 splicing junctions. 137662 known splicing junctions. 34720 novel splicing junctions.
sampling 80% (9391771) splicing reads. 174105 splicing junctions. 138356 known splicing junctions. 35749 novel splicing junctions.
sampling 85% (9978756) splicing reads. 175734 splicing junctions. 139029 known splicing junctions. 36705 novel splicing junctions.
sampling 90% (10565742) splicing reads. 177220 splicing junctions. 139630 known splicing junctions. 37590 novel splicing junctions.
sampling 95% (11152728) splicing reads. 178499 splicing junctions. 140146 known splicing junctions. 38353 novel splicing junctions.
sampling 100% (11739714) splicing reads. 179686 splicing junctions. 140590 known splicing junctions. 39096 novel splicing junctions.
null device 
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