Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ec/3ce35b6cf204cdf6b19a49b1530c9c/rna_s1221_S42_1.fastp.fastq.gz
Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4e/ab1e5250667d30349a295b17f62f25/.command.sh
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0d/f9f14c573c32bd7ba2c65185b5c887/kallisto
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4e/ab1e5250667d30349a295b17f62f25/.command.run
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ec/3ce35b6cf204cdf6b19a49b1530c9c/rna_s1221_S42_2.fastp.fastq.gz
==> STAGING COMPLETE (6 inputs)
[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 194,360
[index] number of k-mers: 112,035,259
[quant] running in paired-end mode
[quant] will process pair 1: rna_s1221_S42_1.fastp.fastq.gz
rna_s1221_S42_2.fastp.fastq.gz
[quant] finding pseudoalignments for the reads ...
[progress] 1M reads processed (96.4% mapped)
[progress] 2M reads processed (96.4% mapped)
[progress] 3M reads processed (96.4% mapped)
[progress] 4M reads processed (96.4% mapped)
[progress] 5M reads processed (96.4% mapped)
[progress] 6M reads processed (96.4% mapped)
[progress] 7M reads processed (96.4% mapped)
[progress] 8M reads processed (96.4% mapped)
[progress] 9M reads processed (96.4% mapped) done
[quant] processed 9,932,006 reads, 9,571,272 reads pseudoaligned
[quant] estimated average fragment length: 175.4
[ em] quantifying the abundances ... done
[ em] the Expectation-Maximization algorithm ran for 1,263 rounds