Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/f87e9d06b362fee22bd04072d1a86d/rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b7/7b351bc9499447045b38725902b2cd/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/25/0bc0240ab6d92e79f0c1583b0da341/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/25/0bc0240ab6d92e79f0c1583b0da341/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/00/06a772fa6915b0e577c8e642311ab0/rna_s1221_S42.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (560870) splicing reads. 94046 splicing junctions. 89661 known splicing junctions. 4385 novel splicing junctions.
sampling 10% (1121740) splicing reads. 112964 splicing junctions. 105021 known splicing junctions. 7943 novel splicing junctions.
sampling 15% (1682611) splicing reads. 123903 splicing junctions. 112800 known splicing junctions. 11103 novel splicing junctions.
sampling 20% (2243481) splicing reads. 131454 splicing junctions. 117588 known splicing junctions. 13866 novel splicing junctions.
sampling 25% (2804352) splicing reads. 137590 splicing junctions. 121190 known splicing junctions. 16400 novel splicing junctions.
sampling 30% (3365222) splicing reads. 142784 splicing junctions. 124020 known splicing junctions. 18764 novel splicing junctions.
sampling 35% (3926092) splicing reads. 147112 splicing junctions. 126287 known splicing junctions. 20825 novel splicing junctions.
sampling 40% (4486963) splicing reads. 150828 splicing junctions. 128166 known splicing junctions. 22662 novel splicing junctions.
sampling 45% (5047833) splicing reads. 154287 splicing junctions. 129855 known splicing junctions. 24432 novel splicing junctions.
sampling 50% (5608704) splicing reads. 157328 splicing junctions. 131272 known splicing junctions. 26056 novel splicing junctions.
sampling 55% (6169574) splicing reads. 160184 splicing junctions. 132555 known splicing junctions. 27629 novel splicing junctions.
sampling 60% (6730444) splicing reads. 162660 splicing junctions. 133648 known splicing junctions. 29012 novel splicing junctions.
sampling 65% (7291315) splicing reads. 164936 splicing junctions. 134619 known splicing junctions. 30317 novel splicing junctions.
sampling 70% (7852185) splicing reads. 166988 splicing junctions. 135436 known splicing junctions. 31552 novel splicing junctions.
sampling 75% (8413056) splicing reads. 168820 splicing junctions. 136184 known splicing junctions. 32636 novel splicing junctions.
sampling 80% (8973926) splicing reads. 170473 splicing junctions. 136867 known splicing junctions. 33606 novel splicing junctions.
sampling 85% (9534796) splicing reads. 172029 splicing junctions. 137510 known splicing junctions. 34519 novel splicing junctions.
sampling 90% (10095667) splicing reads. 173419 splicing junctions. 138089 known splicing junctions. 35330 novel splicing junctions.
sampling 95% (10656537) splicing reads. 174692 splicing junctions. 138570 known splicing junctions. 36122 novel splicing junctions.
sampling 100% (11217408) splicing reads. 175815 splicing junctions. 139001 known splicing junctions. 36814 novel splicing junctions.
null device 
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