File Info

Filename
multiqc_data/multiqc_data.json
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/da/bf0042673a7eacd8913f6f6386c1ed/multiqc_data/multiqc_data.json
Size
4.3 MB
Attempt
{
  "report_data_sources": {
    "Kallisto": {
      "all_sections": {
        "rna_s1221_S42_1": "/tmp/nxf.CRgH8grVmo/16/rna_s1221_S42.log",
        "rna_s1200_S21_1": "/tmp/nxf.CRgH8grVmo/15/rna_s1200_S21.log"
      }
    },
    "Picard": {
      "InsertSizeMetrics": {
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/24/rna_s1221_S42_collectinsertsize.txt",
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/23/rna_s1200_S21_collectinsertsize.txt"
      },
      "DuplicationMetrics": {
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/40/rna_s1221_S42.md.bam.metrics",
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/39/rna_s1200_S21.md.bam.metrics"
      },
      "RnaSeqMetrics": {
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/53/rna_s1221_S42_rna_metrics.txt",
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/54/rna_s1200_S21_rna_metrics.txt"
      }
    },
    "Preseq": {
      "all_sections": {
        "rna_s1200_S21.lc_extrap": "/tmp/nxf.CRgH8grVmo/27/rna_s1200_S21.lc_extrap.txt",
        "rna_s1221_S42.lc_extrap": "/tmp/nxf.CRgH8grVmo/28/rna_s1221_S42.lc_extrap.txt"
      }
    },
    "RSeQC": {
      "read_distribution": {
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/45/rna_s1200_S21.read_distribution.txt",
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/46/rna_s1221_S42.read_distribution.txt"
      },
      "inner_distance": {
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/35/rna_s1200_S21.inner_distance_freq.txt",
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/36/rna_s1221_S42.inner_distance_freq.txt"
      },
      "read_duplication": {
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/43/rna_s1200_S21.pos.DupRate.xls",
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/44/rna_s1221_S42.pos.DupRate.xls"
      },
      "junction_annotation": {
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/37/rna_s1200_S21.junction_annotation.log",
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/38/rna_s1221_S42.junction_annotation.log"
      },
      "junction_saturation": {
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/42/rna_s1221_S42.junctionSaturation_plot.r",
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/41/rna_s1200_S21.junctionSaturation_plot.r"
      },
      "infer_experiment": {
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/34/rna_s1221_S42.infer_experiment.txt",
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/33/rna_s1200_S21.infer_experiment.txt"
      },
      "bam_stat": {
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/30/rna_s1221_S42.bam_stat.txt",
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/29/rna_s1200_S21.bam_stat.txt"
      }
    },
    "STAR": {
      "SummaryLog": {
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/19/rna_s1200_S21.Log.final.out",
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/20/rna_s1221_S42.Log.final.out"
      },
      "ReadsPerGene": {
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/22/rna_s1221_S42.ReadsPerGene.out.tab",
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/21/rna_s1200_S21.ReadsPerGene.out.tab"
      }
    },
    "fastp": {
      "all_sections": {
        "rna_s1221_S42": "/tmp/nxf.CRgH8grVmo/4/rna_s1221_S42.fastp.json",
        "rna_s1200_S21": "/tmp/nxf.CRgH8grVmo/3/rna_s1200_S21.fastp.json"
      }
    },
    "FastQC (raw)": {
      "all_sections": {
        "rna_s1221_S42_trimmed_1": "/tmp/nxf.CRgH8grVmo/13/rna_s1221_S42_trimmed_1_fastqc.zip",
        "rna_s1200_S21_trimmed_2": "/tmp/nxf.CRgH8grVmo/12/rna_s1200_S21_trimmed_2_fastqc.zip",
        "rna_s1221_S42_1": "/tmp/nxf.CRgH8grVmo/9/rna_s1221_S42_1_fastqc.zip",
        "rna_s1200_S21_1": "/tmp/nxf.CRgH8grVmo/7/rna_s1200_S21_1_fastqc.zip",
        "rna_s1221_S42_trimmed_2": "/tmp/nxf.CRgH8grVmo/14/rna_s1221_S42_trimmed_2_fastqc.zip",
        "rna_s1221_S42_2": "/tmp/nxf.CRgH8grVmo/10/rna_s1221_S42_2_fastqc.zip",
        "rna_s1200_S21_trimmed_1": "/tmp/nxf.CRgH8grVmo/11/rna_s1200_S21_trimmed_1_fastqc.zip",
        "rna_s1200_S21_2": "/tmp/nxf.CRgH8grVmo/8/rna_s1200_S21_2_fastqc.zip"
      }
    },
    "FastQC (trimmed)": {
      "all_sections": {
        "rna_s1221_S42_trimmed_1": "/tmp/nxf.CRgH8grVmo/13/rna_s1221_S42_trimmed_1_fastqc.zip",
        "rna_s1200_S21_trimmed_2": "/tmp/nxf.CRgH8grVmo/12/rna_s1200_S21_trimmed_2_fastqc.zip",
        "rna_s1221_S42_trimmed_2": "/tmp/nxf.CRgH8grVmo/14/rna_s1221_S42_trimmed_2_fastqc.zip",
        "rna_s1200_S21_trimmed_1": "/tmp/nxf.CRgH8grVmo/11/rna_s1200_S21_trimmed_1_fastqc.zip"
      }
    }
  },
  "report_general_stats_data": [
    {
      "rna_s1200_S21": {
        "unique_reads_rseqc": 19200129.0,
        "frac_antisense": 0.9607
      },
      "rna_s1221_S42": {
        "unique_reads_rseqc": 18864613.0,
        "frac_antisense": 0.9584
      }
    },
    {
      "rna_s1221_S42": {
        "reads": 19864012.0,
        "frac_unmapped": 0.004391,
        "frac_dupes": 0.198705,
        "unique_reads": 15916933.0,
        "frac_reads_on_target": 0.936077,
        "frac_pairs_on_target": 0.962008,
        "frac_reads_off_target": 0.058443,
        "frac_pairs_off_target": 0.037992,
        "reads_on_target": 14899481.0,
        "pairs_on_target": 7595968.0,
        "reads_off_target": 930229.0,
        "pairs_off_target": 299983.0,
        "mean_depth": 32.870035,
        "q50_depth": 5.0,
        "frac_bases_gt30x": 0.28172,
        "frac_bases_gt100x": 0.079333,
        "n_q30": 0.98502,
        "gc_bias": 0.0
      },
      "rna_s1200_S21": {
        "reads": 19875412.0,
        "frac_unmapped": 0.003853,
        "frac_dupes": 0.183621,
        "unique_reads": 16225876.0,
        "frac_reads_on_target": 0.945347,
        "frac_pairs_on_target": 0.972121,
        "frac_reads_off_target": 0.049933,
        "frac_pairs_off_target": 0.027879,
        "reads_on_target": 15339088.0,
        "pairs_on_target": 7832369.0,
        "reads_off_target": 810206.0,
        "pairs_off_target": 224622.0,
        "mean_depth": 34.559207,
        "q50_depth": 5.0,
        "frac_bases_gt30x": 0.293198,
        "frac_bases_gt100x": 0.084685,
        "n_q30": 0.985625,
        "gc_bias": 0.0
      }
    },
    {
      "rna_s1221_S42": {
        "is_contaminated": "Skipped",
        "contamination_pct": "N/A"
      },
      "rna_s1200_S21": {
        "is_contaminated": "Skipped",
        "contamination_pct": "N/A"
      }
    },
    {
      "rna_s1221_S42": {
        "fragment_length_mean": 175.5,
        "fragment_length_median": 161.0,
        "pct_mito_est_counts": 0.005,
        "pct_ribo_est_counts": 4.713,
        "pct_hk_est_counts": 2.826,
        "pct_male_est_counts": 0.0,
        "pct_mito_tpm": 0.009,
        "pct_ribo_tpm": 11.321,
        "pct_hk_tpm": 3.189,
        "pct_male_tpm": 0.0,
        "score_log2tpm1_mean_chry_signature": 6.774
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      "rna_s1200_S21": {
        "fragment_length_mean": 181.1,
        "fragment_length_median": 166.0,
        "pct_mito_est_counts": 0.002,
        "pct_ribo_est_counts": 4.584,
        "pct_hk_est_counts": 2.972,
        "pct_male_est_counts": 0.0,
        "pct_mito_tpm": 0.003,
        "pct_ribo_tpm": 10.895,
        "pct_hk_tpm": 3.446,
        "pct_male_tpm": 0.0,
        "score_log2tpm1_mean_chry_signature": 6.696
      }
    },
    {
      "rna_s1221_S42": {
        "total_reads": 9999000000.0,
        "expected_distinct": 53000545.0
      },
      "rna_s1200_S21": {
        "total_reads": 9999000000.0,
        "expected_distinct": 53979070.0
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    },
    {
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        "total_reads": 9932006.0,
        "pseudoaligned_reads": 9571272.0,
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        "percent_aligned": 96.36796433671103,
        "fragment_length": 175.5
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      "rna_s1200_S21_1": {
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        "pseudoaligned_reads": 9721684.0,
        "not_pseudoaligned_reads": 216022.0,
        "percent_aligned": 97.82623877180508,
        "fragment_length": 181.1
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    },
    {
      "rna_s1221_S42": {
        "total_count": 8126131,
        "meansum": 6522725284.939242,
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      "rna_s1200_S21": {
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    },
    {
      "rna_s1221_S42": {
        "LIBRARY": "Unknown Library",
        "UNPAIRED_READS_EXAMINED": 3.0,
        "READ_PAIRS_EXAMINED": 9888393.0,
        "SECONDARY_OR_SUPPLEMENTARY_RDS": 3584450.0,
        "UNMAPPED_READS": 87223.0,
        "UNPAIRED_READ_DUPLICATES": 3.0,
        "READ_PAIR_DUPLICATES": 1973538.0,
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        "PERCENT_DUPLICATION": 0.199581,
        "ESTIMATED_LIBRARY_SIZE": 21499970.0,
        "READS_IN_DUPLICATE_PAIRS": 3947076.0,
        "READS_IN_UNIQUE_PAIRS": 15829710.0,
        "READS_IN_UNIQUE_UNPAIRED": 0.0,
        "READS_IN_DUPLICATE_PAIRS_OPTICAL": 36510.0,
        "READS_IN_DUPLICATE_PAIRS_NONOPTICAL": 3910566.0,
        "READS_IN_DUPLICATE_UNPAIRED": 3.0,
        "READS_UNMAPPED": 87223.0
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      "rna_s1200_S21": {
        "LIBRARY": "Unknown Library",
        "UNPAIRED_READS_EXAMINED": 2.0,
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        "UNMAPPED_READS": 76582.0,
        "UNPAIRED_READ_DUPLICATES": 2.0,
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        "PERCENT_DUPLICATION": 0.184331,
        "ESTIMATED_LIBRARY_SIZE": 23557470.0,
        "READS_IN_DUPLICATE_PAIRS": 3649534.0,
        "READS_IN_UNIQUE_PAIRS": 16149294.0,
        "READS_IN_UNIQUE_UNPAIRED": 0.0,
        "READS_IN_DUPLICATE_PAIRS_OPTICAL": 24134.0,
        "READS_IN_DUPLICATE_PAIRS_NONOPTICAL": 3625400.0,
        "READS_IN_DUPLICATE_UNPAIRED": 2.0,
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    {
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        "INTRONIC_BASES": 94951215.0,
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        "NUM_R1_TRANSCRIPT_STRAND_READS": 82025.0,
        "NUM_R2_TRANSCRIPT_STRAND_READS": 8016569.0,
        "NUM_UNEXPLAINED_READS": 44319.0,
        "PCT_R1_TRANSCRIPT_STRAND_READS": 1.0128,
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        "PCT_RIBOSOMAL_BASES": 0.0873,
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        "PCT_INTRONIC_BASES": 3.4812000000000003,
        "PCT_INTERGENIC_BASES": 1.176,
        "PCT_MRNA_BASES": 95.2555,
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        "PCT_CORRECT_STRAND_READS": 0.0,
        "MEDIAN_CV_COVERAGE": 0.579755,
        "MEDIAN_5PRIME_BIAS": 0.568567,
        "MEDIAN_3PRIME_BIAS": 0.670525,
        "MEDIAN_5PRIME_TO_3PRIME_BIAS": 0.715967,
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        "NUM_R1_TRANSCRIPT_STRAND_READS": 128383.0,
        "NUM_R2_TRANSCRIPT_STRAND_READS": 8100715.0,
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        "MEDIAN_5PRIME_BIAS": 0.543049,
        "MEDIAN_3PRIME_BIAS": 0.68164,
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        "LIBRARY": "NA",
        "READ_GROUP": "NA",
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    },
    {
      "rna_s1221_S42": {
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        "qc_failed": 0,
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        "non_primary_hits": 3505214,
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        "mapq_lt_mapq_cut_non-unique": 991412,
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      "rna_s1200_S21": {
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    {
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        "mismatch_rate": 0.15,
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      "rna_s1221_S42": {
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        "multimapped_percent": 4.23,
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        "unmapped_mismatches": 30672,
        "unmapped_tooshort": 97428,
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    },
    {
      "rna_s1221_S42": {
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        "pct_duplication": 13.947899999999999,
        "after_filtering_total_reads": 19864012.0,
        "after_filtering_total_bases": 2750073323.0,
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        "after_filtering_q20_rate": 0.995114,
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        "after_filtering_read1_mean_length": 138.0,
        "after_filtering_read2_mean_length": 138.0,
        "after_filtering_gc_content": 0.533166,
        "before_filtering_total_reads": 20000000.0,
        "pct_surviving": 99.32006,
        "adapter_cutting_adapter_trimmed_reads": 7366518.0,
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      "rna_s1200_S21": {
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        "filtering_result_too_short_reads": 38.0,
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        "pct_duplication": 13.3191,
        "after_filtering_total_reads": 19875412.0,
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        "colour": "255,127,0",
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      "pct_mito_tpm": {
        "namespace": "Custom content: Expression QC",
        "title": "pct Mito TPM",
        "description": "Percentage of TPM from mitochondrial genes; high values can indicate cell damage or stress.",
        "format": "{:,.2f}",
        "suffix": "%",
        "rid": "mqc-generalstats-custom_content_expression_qc-pct_mito_tpm",
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        "namespace": "Custom content: Expression QC",
        "title": "pct Ribo TPM",
        "description": "Percentage of TPM from ribosomal genes; useful for assessing RNA quality and technical variation.",
        "format": "{:,.2f}",
        "suffix": "%",
        "rid": "mqc-generalstats-custom_content_expression_qc-pct_ribo_tpm",
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        "title": "pct Housekeeping TPM",
        "description": "Percentage of TPM from housekeeping genes.",
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        "suffix": "%",
        "rid": "mqc-generalstats-custom_content_expression_qc-pct_hk_tpm",
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        "description": "pct Male TPM",
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        "namespace": "Custom content: Expression QC",
        "title": "ChrY Signature Score",
        "description": "Mean log2(TPM+1) score for chrY signature genes; used for sex verification.",
        "format": "{:,.2f}",
        "rid": "mqc-generalstats-custom_content_expression_qc-score_log2tpm1_mean_chry_signature",
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        "title": "Expected Distinct",
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        "scale": "YlGn",
        "namespace": "Kallisto",
        "rid": "mqc-generalstats-kallisto-percent_aligned",
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        "description": "Median Insert Size, all read orientations (bp)",
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        "namespace": "Picard: InsertSizeMetrics",
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      "summed_mean": {
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        "description": "Mean Insert Size, all read orientations (bp)",
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        "hidden": true,
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        "title": "Duplication",
        "description": "Mark Duplicates - Percent Duplication",
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        "suffix": "%",
        "scale": "OrRd",
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        "namespace": "Picard: Mark Duplicates",
        "rid": "mqc-generalstats-picard_mark_duplicates-PERCENT_DUPLICATION",
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        "description": "Percent of aligned bases overlapping ribosomal RNA regions",
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        "scale": "Reds",
        "namespace": "Picard: RnaSeqMetrics",
        "rid": "mqc-generalstats-picard_rnaseqmetrics-PCT_RIBOSOMAL_BASES",
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        "scale": "Greens",
        "namespace": "Picard: RnaSeqMetrics",
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        "description": "% Reads mapped in proper pairs",
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        "scale": "RdYlGn",
        "namespace": "RSeQC",
        "rid": "mqc-generalstats-rseqc-proper_pairs_percent",
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        "description": "% Uniquely mapped reads",
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        "scale": "YlGn",
        "namespace": "STAR",
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        "description": "Uniquely mapped reads (millions)",
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        "description": "Duplication rate before filtering",
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        "scale": "RdYlGn-rev",
        "namespace": "fastp",
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        "description": "Percentage of reads > Q30 after filtering",
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        "description": "Bases > Q30 after filtering (millions)",
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        "title": "M Reads After Filtering",
        "description": "Total reads after filtering (millions)",
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        "modify": 1e-06,
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        "namespace": "fastp",
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        "scale": "RdYlGn-rev",
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        "description": "Average % GC Content",
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            "GGTAA": 718929,
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            "GGTCT": 1871309,
            "GGTCC": 1886465,
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            "GGCGA": 960308,
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            "GGGCT": 2534934,
            "GGGCC": 2576366,
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            "GGGGT": 2307947,
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            "GGGGG": 3873313
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          "overrepresented_sequences": {}
        },
        "read2_before_filtering": {
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          "total_bases": 1500000000,
          "q20_bases": 1482009250,
          "q30_bases": 1453828999,
          "total_cycles": 150,
          "quality_curves": {
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            "GTGGT": 2137933,
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            "GCGTC": 3376846,
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            "GCGCA": 921064,
            "GCGCT": 991185,
            "GCGCC": 1521381,
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            "GCGGT": 709635,
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            "GGAAA": 4478389,
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            "GGACG": 1058812,
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            "GGAGT": 1424441,
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            "GGTAA": 525904,
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            "GGTCA": 1164710,
            "GGTCT": 940962,
            "GGTCC": 1137005,
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            "GGTGA": 1694363,
            "GGTGT": 1244717,
            "GGTGC": 1621065,
            "GGTGG": 3068470,
            "GGCAA": 1780178,
            "GGCAT": 1344726,
            "GGCAC": 1394826,
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            "GGCTT": 1347007,
            "GGCTC": 1840714,
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            "GGCCA": 2780568,
            "GGCCT": 2029274,
            "GGCCC": 2678999,
            "GGCCG": 1909658,
            "GGCGA": 858322,
            "GGCGT": 673474,
            "GGCGC": 1376162,
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            "GGGAA": 3836278,
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            "GGGTC": 980106,
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            "GGGCA": 1835379,
            "GGGCT": 1633096,
            "GGGCC": 2293015,
            "GGGCG": 1257652,
            "GGGGA": 1673006,
            "GGGGT": 825545,
            "GGGGC": 1909531,
            "GGGGG": 8262316
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          "overrepresented_sequences": {}
        },
        "read1_after_filtering": {
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          "total_bases": 1374926771,
          "q20_bases": 1369172921,
          "q30_bases": 1355291933,
          "total_cycles": 150,
          "quality_curves": {
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            "TTTTT": 3188485,
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            "TTTCA": 2393076,
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            "CCGTC": 861742,
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            "GACAG": 1564704,
            "GACTA": 303030,
            "GACTT": 1257322,
            "GACTC": 1121079,
            "GACTG": 1282175,
            "GACCA": 1175233,
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            "GACCC": 942258,
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            "GACGC": 644077,
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            "GAGAT": 1163493,
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            "GAGAG": 1521610,
            "GAGTA": 647269,
            "GAGTT": 1066108,
            "GAGTC": 1132814,
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            "GAGCA": 1693624,
            "GAGCT": 1981913,
            "GAGCC": 1732239,
            "GAGCG": 810167,
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            "GAGGC": 2017127,
            "GAGGG": 1894374,
            "GTAAA": 714882,
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            "GTATA": 367509,
            "GTATT": 740045,
            "GTATC": 581713,
            "GTATG": 480614,
            "GTACA": 937800,
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            "GTACC": 581698,
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            "GTAGA": 1085335,
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            "GTTAA": 488923,
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            "GTTAG": 414051,
            "GTTTA": 664771,
            "GTTTT": 1828211,
            "GTTTC": 1762290,
            "GTTTG": 1333189,
            "GTTCA": 1533527,
            "GTTCT": 2082713,
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            "GTCAG": 1717132,
            "GTCTA": 430618,
            "GTCTT": 2094472,
            "GTCTC": 1654976,
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            "GTCCA": 2142825,
            "GTCCT": 1948942,
            "GTCCC": 1437181,
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            "GTGAA": 1161279,
            "GTGAT": 1200591,
            "GTGAC": 1130778,
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            "GTGTA": 741957,
            "GTGTT": 1166870,
            "GTGTC": 1226449,
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            "GTGCA": 1296294,
            "GTGCT": 1788684,
            "GTGCC": 1389563,
            "GTGCG": 610650,
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            "GTGGT": 1771621,
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            "GTGGG": 1860729,
            "GCAAA": 1250067,
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            "GCACA": 1547932,
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            "GCACC": 1551855,
            "GCACG": 920230,
            "GCAGA": 1872170,
            "GCAGT": 1580047,
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            "GCTAA": 425115,
            "GCTAT": 438803,
            "GCTAC": 498947,
            "GCTAG": 428425,
            "GCTTA": 547491,
            "GCTTT": 2039065,
            "GCTTC": 2823132,
            "GCTTG": 2143839,
            "GCTCA": 1805763,
            "GCTCT": 2222132,
            "GCTCC": 2971363,
            "GCTCG": 1036833,
            "GCTGA": 2107163,
            "GCTGT": 2359571,
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            "GCTGG": 3631221,
            "GCCAA": 1342387,
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            "GCCTA": 327010,
            "GCCTT": 2174702,
            "GCCTC": 2335800,
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            "GCCCT": 1679944,
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            "GCGAA": 431873,
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            "GCGAG": 772234,
            "GCGTA": 342148,
            "GCGTT": 494117,
            "GCGTC": 744702,
            "GCGTG": 785012,
            "GCGCA": 825927,
            "GCGCT": 1096958,
            "GCGCC": 1239945,
            "GCGCG": 802652,
            "GCGGA": 964814,
            "GCGGT": 932745,
            "GCGGC": 1957358,
            "GCGGG": 1446417,
            "GGAAA": 1310175,
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            "GGATA": 672160,
            "GGATT": 1023349,
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            "GGACA": 1510186,
            "GGACT": 1255047,
            "GGACC": 1092550,
            "GGACG": 735754,
            "GGAGA": 1878903,
            "GGAGT": 1278792,
            "GGAGC": 2124359,
            "GGAGG": 2777145,
            "GGTAA": 712857,
            "GGTAT": 629089,
            "GGTAC": 879936,
            "GGTAG": 1254760,
            "GGTTA": 474753,
            "GGTTT": 1483242,
            "GGTTC": 1443401,
            "GGTTG": 1455806,
            "GGTCA": 1788773,
            "GGTCT": 1851580,
            "GGTCC": 1873163,
            "GGTCG": 728541,
            "GGTGA": 1906406,
            "GGTGT": 1462009,
            "GGTGC": 1801033,
            "GGTGG": 2864331,
            "GGCAA": 1290830,
            "GGCAT": 1598636,
            "GGCAC": 1639743,
            "GGCAG": 3106534,
            "GGCTA": 525071,
            "GGCTT": 2086695,
            "GGCTC": 2189738,
            "GGCTG": 3459409,
            "GGCCA": 2725075,
            "GGCCT": 2474326,
            "GGCCC": 2225708,
            "GGCCG": 1620886,
            "GGCGA": 936387,
            "GGCGT": 916416,
            "GGCGC": 1402710,
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            "GGGAA": 1445107,
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            "GGGAC": 1268103,
            "GGGAG": 2107997,
            "GGGTA": 763303,
            "GGGTT": 1226903,
            "GGGTC": 1660009,
            "GGGTG": 1978282,
            "GGGCA": 2361345,
            "GGGCT": 2516387,
            "GGGCC": 2559138,
            "GGGCG": 1280130,
            "GGGGA": 1779552,
            "GGGGT": 1775646,
            "GGGGC": 2647539,
            "GGGGG": 2495019
          },
          "overrepresented_sequences": {}
        },
        "read2_after_filtering": {
          "total_reads": 9932006,
          "total_bases": 1375146552,
          "q20_bases": 1367462784,
          "q30_bases": 1350487305,
          "total_cycles": 150,
          "quality_curves": {
            "A": [
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              38.9862,
              39.1519,
              39.4448,
              39.4365,
              39.489,
              39.4826,
              39.5028,
              39.5463,
              39.5743,
              39.5864,
              39.6105,
              39.6316,
              39.6155,
              39.5719,
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            "TACAT": 790266,
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            "TACTG": 979707,
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            "TACCT": 1036979,
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            "TAGAT": 476519,
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            "TAGTA": 295159,
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            "TAGCA": 629284,
            "TAGCT": 480080,
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            "TAGGC": 318163,
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            "TTAAC": 458701,
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            "TTATG": 844510,
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            "TTTAA": 883934,
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            "TTTTT": 1397149,
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            "TTTCA": 1282938,
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            "TTTGA": 1782339,
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            "TTCCC": 1420555,
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            "TTCGT": 539610,
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            "TTGGT": 1074417,
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            "TCACG": 503165,
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            "TCTAC": 1068189,
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            "TCCAC": 1604775,
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            "TCCGC": 1041676,
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            "TCGCA": 472425,
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            "TCGGA": 660733,
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            "TCGGC": 814982,
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            "TGAAT": 1249585,
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            "TGATG": 2547035,
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            "TGACG": 694877,
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            "TGAGT": 1002455,
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            "TGTAA": 641528,
            "TGTAT": 735684,
            "TGTAC": 917283,
            "TGTAG": 559059,
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            "TGCAT": 969224,
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            "TGCGC": 861962,
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            "TGGAC": 2131479,
            "TGGAG": 3751769,
            "TGGTA": 814532,
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            "TGGTG": 2761285,
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            "TGGCG": 1135449,
            "TGGGA": 1798407,
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            "TGGGC": 1989512,
            "TGGGG": 1720080,
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            "CAATT": 709916,
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            "CAATG": 1504022,
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            "CAAGT": 1639775,
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            "CACCT": 1958809,
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            "CAGTC": 1298614,
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            "CAGGT": 1503301,
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            "CTAAA": 885376,
            "CTAAT": 447556,
            "CTAAC": 395788,
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            "CTATA": 432400,
            "CTATT": 633623,
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            "CTACA": 1535464,
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            "CTACC": 1236456,
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            "CTTTT": 1297968,
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            "CTCAT": 1341838,
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            "CTCCA": 2525258,
            "CTCCT": 2467976,
            "CTCCC": 2095320,
            "CTCCG": 1176715,
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            "CTCGT": 538140,
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            "CTGTA": 956530,
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            "CTGTC": 1583097,
            "CTGTG": 2566811,
            "CTGCA": 2797051,
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            "CTGCG": 1346442,
            "CTGGA": 3708726,
            "CTGGT": 1972042,
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            "CTGGG": 2524190,
            "CCAAA": 1951728,
            "CCAAT": 954252,
            "CCAAC": 1632119,
            "CCAAG": 3022154,
            "CCATA": 504437,
            "CCATT": 1187324,
            "CCATC": 2126021,
            "CCATG": 2078080,
            "CCACA": 1886363,
            "CCACT": 1439769,
            "CCACC": 2814535,
            "CCACG": 1050118,
            "CCAGA": 2900682,
            "CCAGT": 1847153,
            "CCAGC": 3633993,
            "CCAGG": 2939290,
            "CCTAA": 639102,
            "CCTAT": 672512,
            "CCTAC": 1172764,
            "CCTAG": 517931,
            "CCTTA": 628382,
            "CCTTT": 1322684,
            "CCTTC": 2158454,
            "CCTTG": 1319311,
            "CCTCA": 2160547,
            "CCTCT": 1843855,
            "CCTCC": 2861125,
            "CCTCG": 1052263,
            "CCTGA": 2443562,
            "CCTGT": 1812352,
            "CCTGC": 3184999,
            "CCTGG": 3759292,
            "CCCAA": 1745065,
            "CCCAT": 1195029,
            "CCCAC": 1828926,
            "CCCAG": 3356751,
            "CCCTA": 713966,
            "CCCTT": 1215837,
            "CCCTC": 1910345,
            "CCCTG": 3000284,
            "CCCCA": 2447577,
            "CCCCT": 1744995,
            "CCCCC": 2709893,
            "CCCCG": 1730200,
            "CCCGA": 1005558,
            "CCCGT": 727437,
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            "CCGAA": 749509,
            "CCGAT": 390881,
            "CCGAC": 778510,
            "CCGAG": 1600700,
            "CCGTA": 303941,
            "CCGTT": 377226,
            "CCGTC": 859952,
            "CCGTG": 1264611,
            "CCGCA": 1219357,
            "CCGCT": 1102737,
            "CCGCC": 2219534,
            "CCGCG": 1146049,
            "CCGGA": 1128791,
            "CCGGT": 534890,
            "CCGGC": 1779787,
            "CCGGG": 1567560,
            "CGAAA": 640644,
            "CGAAT": 406355,
            "CGAAC": 351735,
            "CGAAG": 849328,
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            "CGATT": 301029,
            "CGATC": 333575,
            "CGATG": 706637,
            "CGACA": 809020,
            "CGACT": 545428,
            "CGACC": 755620,
            "CGACG": 602654,
            "CGAGA": 1221796,
            "CGAGT": 745447,
            "CGAGC": 1112250,
            "CGAGG": 1498900,
            "CGTAA": 232325,
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            "CGTAC": 276081,
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            "CGCAA": 838605,
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            "CGCAC": 617078,
            "CGCAG": 1129254,
            "CGCTA": 401735,
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            "CGCTC": 856853,
            "CGCTG": 1431003,
            "CGCCA": 1399961,
            "CGCCT": 1241751,
            "CGCCC": 1351389,
            "CGCCG": 1555147,
            "CGCGA": 421058,
            "CGCGT": 352714,
            "CGCGC": 909897,
            "CGCGG": 1063885,
            "CGGAA": 951994,
            "CGGAT": 516208,
            "CGGAC": 735529,
            "CGGAG": 1405327,
            "CGGTA": 238922,
            "CGGTT": 364293,
            "CGGTC": 503909,
            "CGGTG": 990523,
            "CGGCA": 1258940,
            "CGGCT": 1239888,
            "CGGCC": 1768923,
            "CGGCG": 2096689,
            "CGGGA": 1386797,
            "CGGGT": 600987,
            "CGGGC": 1408606,
            "CGGGG": 1336814,
            "GAAAA": 3060589,
            "GAAAT": 1793526,
            "GAAAC": 1686232,
            "GAAAG": 2431986,
            "GAATA": 760567,
            "GAATT": 1211233,
            "GAATC": 1032451,
            "GAATG": 1518929,
            "GAACA": 1844432,
            "GAACT": 1388387,
            "GAACC": 1414100,
            "GAACG": 763332,
            "GAAGA": 4945058,
            "GAAGT": 1817797,
            "GAAGC": 2854264,
            "GAAGG": 2920372,
            "GATAA": 824868,
            "GATAT": 762104,
            "GATAC": 569912,
            "GATAG": 475570,
            "GATTA": 638561,
            "GATTT": 1087849,
            "GATTC": 1006078,
            "GATTG": 1012821,
            "GATCA": 1381697,
            "GATCT": 1115668,
            "GATCC": 1367698,
            "GATCG": 614715,
            "GATGA": 2901232,
            "GATGT": 1411532,
            "GATGC": 1678845,
            "GATGG": 2038881,
            "GACAA": 1777143,
            "GACAT": 1377797,
            "GACAC": 1210835,
            "GACAG": 1965190,
            "GACTA": 642633,
            "GACTT": 1261073,
            "GACTC": 1150685,
            "GACTG": 1465915,
            "GACCA": 1879378,
            "GACCT": 1727546,
            "GACCC": 1625114,
            "GACCG": 925868,
            "GACGA": 938188,
            "GACGT": 563694,
            "GACGC": 773410,
            "GACGG": 896866,
            "GAGAA": 3242883,
            "GAGAT": 1802982,
            "GAGAC": 1630691,
            "GAGAG": 2107581,
            "GAGTA": 734745,
            "GAGTT": 1126321,
            "GAGTC": 1122114,
            "GAGTG": 1411644,
            "GAGCA": 2258952,
            "GAGCT": 2394832,
            "GAGCC": 2218611,
            "GAGCG": 1272019,
            "GAGGA": 3868828,
            "GAGGT": 1466228,
            "GAGGC": 2368283,
            "GAGGG": 1583894,
            "GTAAA": 760692,
            "GTAAT": 421416,
            "GTAAC": 411135,
            "GTAAG": 474715,
            "GTATA": 360712,
            "GTATT": 610668,
            "GTATC": 564085,
            "GTATG": 774245,
            "GTACA": 886994,
            "GTACT": 678124,
            "GTACC": 871018,
            "GTACG": 408797,
            "GTAGA": 654362,
            "GTAGT": 377111,
            "GTAGC": 501452,
            "GTAGG": 333712,
            "GTTAA": 528561,
            "GTTAT": 543970,
            "GTTAC": 603362,
            "GTTAG": 323462,
            "GTTTA": 577983,
            "GTTTT": 1020346,
            "GTTTC": 962357,
            "GTTTG": 1267825,
            "GTTCA": 1173641,
            "GTTCT": 1142157,
            "GTTCC": 1280245,
            "GTTCG": 530712,
            "GTTGA": 964136,
            "GTTGT": 722842,
            "GTTGC": 865054,
            "GTTGG": 1095300,
            "GTCAA": 1082488,
            "GTCAT": 1100171,
            "GTCAC": 1088913,
            "GTCAG": 1287394,
            "GTCTA": 483124,
            "GTCTT": 989251,
            "GTCTC": 1135106,
            "GTCTG": 1361315,
            "GTCCA": 1357360,
            "GTCCT": 1443711,
            "GTCCC": 1301967,
            "GTCCG": 713130,
            "GTCGA": 384331,
            "GTCGT": 412950,
            "GTCGC": 530618,
            "GTCGG": 670557,
            "GTGAA": 1951080,
            "GTGAT": 1203178,
            "GTGAC": 1353442,
            "GTGAG": 1259947,
            "GTGTA": 590942,
            "GTGTT": 1096120,
            "GTGTC": 1123654,
            "GTGTG": 1574494,
            "GTGCA": 1389155,
            "GTGCT": 1732940,
            "GTGCC": 1648381,
            "GTGCG": 750842,
            "GTGGA": 2737994,
            "GTGGT": 1716877,
            "GTGGC": 2315677,
            "GTGGG": 1666640,
            "GCAAA": 1793493,
            "GCAAT": 806331,
            "GCAAC": 1263353,
            "GCAAG": 2167237,
            "GCATA": 417086,
            "GCATT": 974562,
            "GCATC": 1525372,
            "GCATG": 1209615,
            "GCACA": 1389965,
            "GCACT": 1116164,
            "GCACC": 1783812,
            "GCACG": 690696,
            "GCAGA": 2859389,
            "GCAGT": 1670903,
            "GCAGC": 3967756,
            "GCAGG": 2442819,
            "GCTAA": 705587,
            "GCTAT": 771297,
            "GCTAC": 1193775,
            "GCTAG": 452647,
            "GCTTA": 505224,
            "GCTTT": 1296322,
            "GCTTC": 1865169,
            "GCTTG": 1005839,
            "GCTCA": 1762343,
            "GCTCT": 1659097,
            "GCTCC": 2184872,
            "GCTCG": 830333,
            "GCTGA": 2639712,
            "GCTGT": 2037419,
            "GCTGC": 3876483,
            "GCTGG": 3545139,
            "GCCAA": 2336024,
            "GCCAT": 1890503,
            "GCCAC": 2082391,
            "GCCAG": 2737109,
            "GCCTA": 759878,
            "GCCTT": 1552727,
            "GCCTC": 2076043,
            "GCCTG": 2691657,
            "GCCCA": 2383471,
            "GCCCT": 2134455,
            "GCCCC": 2648404,
            "GCCCG": 1546409,
            "GCCGA": 1128538,
            "GCCGT": 898929,
            "GCCGC": 2135083,
            "GCCGG": 1541646,
            "GCGAA": 460877,
            "GCGAT": 367921,
            "GCGAC": 699734,
            "GCGAG": 1066703,
            "GCGTA": 198402,
            "GCGTT": 337448,
            "GCGTC": 691575,
            "GCGTG": 848309,
            "GCGCA": 906125,
            "GCGCT": 986536,
            "GCGCC": 1510474,
            "GCGCG": 889408,
            "GCGGA": 1120695,
            "GCGGT": 705476,
            "GCGGC": 2822065,
            "GCGGG": 1536292,
            "GGAAA": 2483158,
            "GGAAT": 1220819,
            "GGAAC": 1562966,
            "GGAAG": 3103949,
            "GGATA": 681486,
            "GGATT": 1014892,
            "GGATC": 1139575,
            "GGATG": 2064441,
            "GGACA": 1924886,
            "GGACT": 1335340,
            "GGACC": 1886585,
            "GGACG": 1052021,
            "GGAGA": 3253804,
            "GGAGT": 1417450,
            "GGAGC": 3032728,
            "GGAGG": 3393186,
            "GGTAA": 521650,
            "GGTAT": 536897,
            "GGTAC": 595119,
            "GGTAG": 475970,
            "GGTTA": 453081,
            "GGTTT": 846697,
            "GGTTC": 939851,
            "GGTTG": 803074,
            "GGTCA": 1152542,
            "GGTCT": 935272,
            "GGTCC": 1130555,
            "GGTCG": 543577,
            "GGTGA": 1671812,
            "GGTGT": 1234013,
            "GGTGC": 1614727,
            "GGTGG": 2608198,
            "GGCAA": 1764187,
            "GGCAT": 1339674,
            "GGCAC": 1388553,
            "GGCAG": 2695418,
            "GGCTA": 849609,
            "GGCTT": 1342316,
            "GGCTC": 1833890,
            "GGCTG": 2883981,
            "GGCCA": 2696287,
            "GGCCT": 2022614,
            "GGCCC": 2664850,
            "GGCCG": 1899703,
            "GGCGA": 838551,
            "GGCGT": 668332,
            "GGCGC": 1367839,
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          "total_bases": 1500000000,
          "q20_bases": 1484512451,
          "q30_bases": 1458809565,
          "total_cycles": 150,
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          "total_bases": 1396572795,
          "q20_bases": 1391108252,
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            "GGAGT": 1292020,
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            "GGTAA": 706299,
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            "GGTCA": 1870164,
            "GGTCT": 1813570,
            "GGTCC": 1938860,
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            "GGTGA": 1931479,
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            "GGTGC": 1865837,
            "GGTGG": 2765037,
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            "GGCGA": 903287,
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            "GGGGT": 1679640,
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            "GGGGG": 1879543
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          "overrepresented_sequences": {}
        },
        "read2_after_filtering": {
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          "total_bases": 1396684695,
          "q20_bases": 1389391946,
          "q30_bases": 1372670092,
          "total_cycles": 150,
          "quality_curves": {
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            "TCATG": 1499707,
            "TCACA": 1208451,
            "TCACT": 1127254,
            "TCACC": 1874977,
            "TCACG": 516781,
            "TCAGA": 2015166,
            "TCAGT": 1333265,
            "TCAGC": 2156619,
            "TCAGG": 1651286,
            "TCTAA": 560486,
            "TCTAT": 705351,
            "TCTAC": 1108827,
            "TCTAG": 469637,
            "TCTTA": 616163,
            "TCTTT": 1371356,
            "TCTTC": 2168758,
            "TCTTG": 1200972,
            "TCTCA": 1375545,
            "TCTCT": 1520208,
            "TCTCC": 1896376,
            "TCTCG": 586709,
            "TCTGA": 1644556,
            "TCTGT": 1472132,
            "TCTGC": 2007465,
            "TCTGG": 2193438,
            "TCCAA": 1595944,
            "TCCAT": 1301124,
            "TCCAC": 1611924,
            "TCCAG": 3005796,
            "TCCTA": 801309,
            "TCCTT": 1424406,
            "TCCTC": 2110635,
            "TCCTG": 3154407,
            "TCCCA": 1708531,
            "TCCCT": 1556215,
            "TCCCC": 1652580,
            "TCCCG": 963647,
            "TCCGA": 689451,
            "TCCGT": 573808,
            "TCCGC": 1044341,
            "TCCGG": 1056105,
            "TCGAA": 499499,
            "TCGAT": 369674,
            "TCGAC": 608353,
            "TCGAG": 912056,
            "TCGTA": 238594,
            "TCGTT": 306468,
            "TCGTC": 667243,
            "TCGTG": 894970,
            "TCGCA": 480259,
            "TCGCT": 655734,
            "TCGCC": 972143,
            "TCGCG": 367235,
            "TCGGA": 673406,
            "TCGGT": 437213,
            "TCGGC": 849892,
            "TCGGG": 802416,
            "TGAAA": 2213461,
            "TGAAT": 1252497,
            "TGAAC": 1519719,
            "TGAAG": 3713457,
            "TGATA": 781284,
            "TGATT": 1065454,
            "TGATC": 1231868,
            "TGATG": 2605931,
            "TGACA": 1768386,
            "TGACT": 1363995,
            "TGACC": 1828503,
            "TGACG": 710285,
            "TGAGA": 1845535,
            "TGAGT": 1059804,
            "TGAGC": 1940450,
            "TGAGG": 2204127,
            "TGTAA": 643879,
            "TGTAT": 751707,
            "TGTAC": 962716,
            "TGTAG": 574262,
            "TGTTA": 675649,
            "TGTTT": 1332931,
            "TGTTC": 1312559,
            "TGTTG": 1395941,
            "TGTCA": 1455806,
            "TGTCT": 1380087,
            "TGTCC": 1610046,
            "TGTCG": 521255,
            "TGTGA": 1693680,
            "TGTGT": 1481502,
            "TGTGC": 1741815,
            "TGTGG": 2664014,
            "TGCAA": 1376131,
            "TGCAT": 1011863,
            "TGCAC": 1347679,
            "TGCAG": 3269807,
            "TGCTA": 944241,
            "TGCTT": 1413586,
            "TGCTC": 1842648,
            "TGCTG": 4357427,
            "TGCCA": 2472474,
            "TGCCT": 1998358,
            "TGCCC": 2481322,
            "TGCCG": 1087114,
            "TGCGA": 514898,
            "TGCGT": 490155,
            "TGCGC": 957967,
            "TGCGG": 1169852,
            "TGGAA": 2753159,
            "TGGAT": 1882848,
            "TGGAC": 2273876,
            "TGGAG": 3996074,
            "TGGTA": 850036,
            "TGGTT": 1194474,
            "TGGTC": 1312981,
            "TGGTG": 2969282,
            "TGGCA": 2446639,
            "TGGCT": 2398661,
            "TGGCC": 3072951,
            "TGGCG": 1119742,
            "TGGGA": 1884127,
            "TGGGT": 1090877,
            "TGGGC": 2241698,
            "TGGGG": 1852334,
            "CAAAA": 1924546,
            "CAAAT": 1196984,
            "CAAAC": 1214114,
            "CAAAG": 2302736,
            "CAATA": 601235,
            "CAATT": 692119,
            "CAATC": 630041,
            "CAATG": 1535779,
            "CAACA": 2046212,
            "CAACT": 1296204,
            "CAACC": 1404936,
            "CAACG": 796250,
            "CAAGA": 3034598,
            "CAAGT": 1652392,
            "CAAGC": 2159857,
            "CAAGG": 2660503,
            "CATAA": 450634,
            "CATAT": 527742,
            "CATAC": 446591,
            "CATAG": 440303,
            "CATTA": 671718,
            "CATTT": 1129961,
            "CATTC": 1034228,
            "CATTG": 1659992,
            "CATCA": 2321992,
            "CATCT": 1751602,
            "CATCC": 2114980,
            "CATCG": 1186745,
            "CATGA": 1698002,
            "CATGT": 1220221,
            "CATGC": 1323812,
            "CATGG": 2155648,
            "CACAA": 1248209,
            "CACAT": 1017063,
            "CACAC": 1218072,
            "CACAG": 2325774,
            "CACTA": 598005,
            "CACTT": 1027619,
            "CACTC": 1029865,
            "CACTG": 2045737,
            "CACCA": 2804484,
            "CACCT": 2004224,
            "CACCC": 1916717,
            "CACCG": 1158961,
            "CACGA": 571021,
            "CACGT": 566228,
            "CACGC": 784800,
            "CACGG": 976274,
            "CAGAA": 3184052,
            "CAGAT": 2106893,
            "CAGAC": 1819615,
            "CAGAG": 2958648,
            "CAGTA": 996192,
            "CAGTT": 1528311,
            "CAGTC": 1314883,
            "CAGTG": 2531896,
            "CAGCA": 3745242,
            "CAGCT": 3262812,
            "CAGCC": 3525353,
            "CAGCG": 1630477,
            "CAGGA": 3045725,
            "CAGGT": 1629815,
            "CAGGC": 2446538,
            "CAGGG": 1921710,
            "CTAAA": 844669,
            "CTAAT": 437353,
            "CTAAC": 379134,
            "CTAAG": 695861,
            "CTATA": 423630,
            "CTATT": 635996,
            "CTATC": 615060,
            "CTATG": 1208532,
            "CTACA": 1517204,
            "CTACT": 990632,
            "CTACC": 1257641,
            "CTACG": 688357,
            "CTAGA": 609745,
            "CTAGT": 370725,
            "CTAGC": 430709,
            "CTAGG": 390689,
            "CTTAA": 602530,
            "CTTAT": 614741,
            "CTTAC": 647572,
            "CTTAG": 467234,
            "CTTTA": 782487,
            "CTTTT": 1322873,
            "CTTTC": 1252693,
            "CTTTG": 2040076,
            "CTTCA": 2397323,
            "CTTCT": 2178085,
            "CTTCC": 2463782,
            "CTTCG": 1078875,
            "CTTGA": 1122618,
            "CTTGT": 966908,
            "CTTGC": 1149624,
            "CTTGG": 1580822,
            "CTCAA": 1517458,
            "CTCAT": 1387163,
            "CTCAC": 1346952,
            "CTCAG": 2360627,
            "CTCTA": 796794,
            "CTCTT": 1515297,
            "CTCTC": 1555282,
            "CTCTG": 2410613,
            "CTCCA": 2545090,
            "CTCCT": 2449494,
            "CTCCC": 2023667,
            "CTCCG": 1238047,
            "CTCGA": 594458,
            "CTCGT": 556925,
            "CTCGC": 804526,
            "CTCGG": 1061370,
            "CTGAA": 2633288,
            "CTGAT": 1561173,
            "CTGAC": 1803628,
            "CTGAG": 2538689,
            "CTGTA": 985523,
            "CTGTT": 1413263,
            "CTGTC": 1673619,
            "CTGTG": 2841906,
            "CTGCA": 3010913,
            "CTGCT": 3587842,
            "CTGCC": 3310442,
            "CTGCG": 1514578,
            "CTGGA": 4023725,
            "CTGGT": 2175500,
            "CTGGC": 3293060,
            "CTGGG": 2815381,
            "CCAAA": 1889922,
            "CCAAT": 946807,
            "CCAAC": 1623408,
            "CCAAG": 3079599,
            "CCATA": 501948,
            "CCATT": 1216520,
            "CCATC": 2173908,
            "CCATG": 2189120,
            "CCACA": 1882838,
            "CCACT": 1473401,
            "CCACC": 2751917,
            "CCACG": 1090419,
            "CCAGA": 2999018,
            "CCAGT": 1892067,
            "CCAGC": 3795495,
            "CCAGG": 3196901,
            "CCTAA": 625991,
            "CCTAT": 681875,
            "CCTAC": 1161610,
            "CCTAG": 523325,
            "CCTTA": 625310,
            "CCTTT": 1365940,
            "CCTTC": 2237563,
            "CCTTG": 1424918,
            "CCTCA": 2230588,
            "CCTCT": 1908135,
            "CCTCC": 2710061,
            "CCTCG": 1037536,
            "CCTGA": 2609327,
            "CCTGT": 1945388,
            "CCTGC": 3485937,
            "CCTGG": 4216754,
            "CCCAA": 1737251,
            "CCCAT": 1236674,
            "CCCAC": 1816994,
            "CCCAG": 3562520,
            "CCCTA": 706065,
            "CCCTT": 1266166,
            "CCCTC": 1882177,
            "CCCTG": 3324244,
            "CCCCA": 2505189,
            "CCCCT": 1790826,
            "CCCCC": 1953202,
            "CCCCG": 1528519,
            "CCCGA": 1019769,
            "CCCGT": 704834,
            "CCCGC": 1565347,
            "CCCGG": 1659323,
            "CCGAA": 681348,
            "CCGAT": 404129,
            "CCGAC": 803684,
            "CCGAG": 1678994,
            "CCGTA": 308988,
            "CCGTT": 387453,
            "CCGTC": 763204,
            "CCGTG": 1372151,
            "CCGCA": 1286048,
            "CCGCT": 1173023,
            "CCGCC": 2183794,
            "CCGCG": 1114509,
            "CCGGA": 1190473,
            "CCGGT": 558196,
            "CCGGC": 1795724,
            "CCGGG": 1631989,
            "CGAAA": 577058,
            "CGAAT": 383880,
            "CGAAC": 339340,
            "CGAAG": 822230,
            "CGATA": 215018,
            "CGATT": 293937,
            "CGATC": 333094,
            "CGATG": 729965,
            "CGACA": 820318,
            "CGACT": 565128,
            "CGACC": 750727,
            "CGACG": 601084,
            "CGAGA": 1191840,
            "CGAGT": 738861,
            "CGAGC": 1202826,
            "CGAGG": 1543746,
            "CGTAA": 219115,
            "CGTAT": 220313,
            "CGTAC": 282328,
            "CGTAG": 239207,
            "CGTTA": 201456,
            "CGTTT": 376935,
            "CGTTC": 425399,
            "CGTTG": 392492,
            "CGTCA": 769351,
            "CGTCT": 686297,
            "CGTCC": 773704,
            "CGTCG": 435044,
            "CGTGA": 869900,
            "CGTGT": 723822,
            "CGTGC": 1037406,
            "CGTGG": 1555476,
            "CGCAA": 830486,
            "CGCAT": 577368,
            "CGCAC": 638971,
            "CGCAG": 1196904,
            "CGCTA": 414216,
            "CGCTT": 634839,
            "CGCTC": 887970,
            "CGCTG": 1591986,
            "CGCCA": 1459847,
            "CGCCT": 1311525,
            "CGCCC": 1343376,
            "CGCCG": 1519833,
            "CGCGA": 413951,
            "CGCGT": 337917,
            "CGCGC": 863222,
            "CGCGG": 1026664,
            "CGGAA": 966828,
            "CGGAT": 533552,
            "CGGAC": 753901,
            "CGGAG": 1437446,
            "CGGTA": 238030,
            "CGGTT": 375088,
            "CGGTC": 502630,
            "CGGTG": 982865,
            "CGGCA": 1258995,
            "CGGCT": 1345601,
            "CGGCC": 1881243,
            "CGGCG": 1308181,
            "CGGGA": 1339392,
            "CGGGT": 574355,
            "CGGGC": 1481361,
            "CGGGG": 1231441,
            "GAAAA": 2856011,
            "GAAAT": 1742183,
            "GAAAC": 1569692,
            "GAAAG": 2272237,
            "GAATA": 740763,
            "GAATT": 1208111,
            "GAATC": 996520,
            "GAATG": 1528944,
            "GAACA": 1786099,
            "GAACT": 1400312,
            "GAACC": 1427350,
            "GAACG": 738168,
            "GAAGA": 4558535,
            "GAAGT": 1847499,
            "GAAGC": 2892564,
            "GAAGG": 2864633,
            "GATAA": 790322,
            "GATAT": 768647,
            "GATAC": 561192,
            "GATAG": 455701,
            "GATTA": 629015,
            "GATTT": 1095604,
            "GATTC": 1012198,
            "GATTG": 1037297,
            "GATCA": 1384639,
            "GATCT": 1153166,
            "GATCC": 1437333,
            "GATCG": 627245,
            "GATGA": 2831647,
            "GATGT": 1478559,
            "GATGC": 1760789,
            "GATGG": 2128276,
            "GACAA": 1740011,
            "GACAT": 1416512,
            "GACAC": 1241719,
            "GACAG": 1996965,
            "GACTA": 647552,
            "GACTT": 1323860,
            "GACTC": 1186522,
            "GACTG": 1550749,
            "GACCA": 1909298,
            "GACCT": 1819074,
            "GACCC": 1680099,
            "GACCG": 893571,
            "GACGA": 887381,
            "GACGT": 592022,
            "GACGC": 808058,
            "GACGG": 896368,
            "GAGAA": 3088755,
            "GAGAT": 1865537,
            "GAGAC": 1649187,
            "GAGAG": 2085311,
            "GAGTA": 736813,
            "GAGTT": 1170183,
            "GAGTC": 1167214,
            "GAGTG": 1447955,
            "GAGCA": 2344701,
            "GAGCT": 2584573,
            "GAGCC": 2360706,
            "GAGCG": 1304617,
            "GAGGA": 3781121,
            "GAGGT": 1535990,
            "GAGGC": 2509526,
            "GAGGG": 1593195,
            "GTAAA": 737613,
            "GTAAT": 410599,
            "GTAAC": 394185,
            "GTAAG": 474311,
            "GTATA": 355073,
            "GTATT": 620701,
            "GTATC": 577541,
            "GTATG": 798356,
            "GTACA": 906096,
            "GTACT": 704387,
            "GTACC": 904070,
            "GTACG": 420175,
            "GTAGA": 642510,
            "GTAGT": 368146,
            "GTAGC": 511941,
            "GTAGG": 344876,
            "GTTAA": 521661,
            "GTTAT": 546260,
            "GTTAC": 564565,
            "GTTAG": 328896,
            "GTTTA": 577094,
            "GTTTT": 1041059,
            "GTTTC": 982416,
            "GTTTG": 1330962,
            "GTTCA": 1189473,
            "GTTCT": 1188928,
            "GTTCC": 1315784,
            "GTTCG": 561839,
            "GTTGA": 967602,
            "GTTGT": 765226,
            "GTTGC": 914440,
            "GTTGG": 1154078,
            "GTCAA": 1103330,
            "GTCAT": 1141914,
            "GTCAC": 1118299,
            "GTCAG": 1342057,
            "GTCTA": 502485,
            "GTCTT": 1042812,
            "GTCTC": 1133611,
            "GTCTG": 1459234,
            "GTCCA": 1431929,
            "GTCCT": 1472656,
            "GTCCC": 1348752,
            "GTCCG": 657621,
            "GTCGA": 393607,
            "GTCGT": 426400,
            "GTCGC": 549639,
            "GTCGG": 602779,
            "GTGAA": 1962954,
            "GTGAT": 1252634,
            "GTGAC": 1427620,
            "GTGAG": 1345334,
            "GTGTA": 615133,
            "GTGTT": 1124811,
            "GTGTC": 1210125,
            "GTGTG": 1702257,
            "GTGCA": 1489002,
            "GTGCT": 1917781,
            "GTGCC": 1805040,
            "GTGCG": 806700,
            "GTGGA": 2857040,
            "GTGGT": 1809210,
            "GTGGC": 2431420,
            "GTGGG": 1809584,
            "GCAAA": 1725793,
            "GCAAT": 815076,
            "GCAAC": 1248072,
            "GCAAG": 2200716,
            "GCATA": 406482,
            "GCATT": 1003542,
            "GCATC": 1602442,
            "GCATG": 1302124,
            "GCACA": 1415734,
            "GCACT": 1153524,
            "GCACC": 1857531,
            "GCACG": 721145,
            "GCAGA": 2935558,
            "GCAGT": 1735379,
            "GCAGC": 3957423,
            "GCAGG": 2640227,
            "GCTAA": 687489,
            "GCTAT": 790423,
            "GCTAC": 1222596,
            "GCTAG": 469745,
            "GCTTA": 520532,
            "GCTTT": 1354723,
            "GCTTC": 2009869,
            "GCTTG": 1075558,
            "GCTCA": 1847500,
            "GCTCT": 1762721,
            "GCTCC": 2315064,
            "GCTCG": 881684,
            "GCTGA": 2793996,
            "GCTGT": 2232809,
            "GCTGC": 4260192,
            "GCTGG": 3944885,
            "GCCAA": 2385436,
            "GCCAT": 1996676,
            "GCCAC": 2137583,
            "GCCAG": 2937447,
            "GCCTA": 776154,
            "GCCTT": 1661726,
            "GCCTC": 2204364,
            "GCCTG": 2998295,
            "GCCCA": 2538937,
            "GCCCT": 2332323,
            "GCCCC": 2633398,
            "GCCCG": 1691101,
            "GCCGA": 1163136,
            "GCCGT": 973444,
            "GCCGC": 2181236,
            "GCCGG": 1619598,
            "GCGAA": 443557,
            "GCGAT": 368966,
            "GCGAC": 680687,
            "GCGAG": 1090153,
            "GCGTA": 195650,
            "GCGTT": 345505,
            "GCGTC": 679324,
            "GCGTG": 925454,
            "GCGCA": 954392,
            "GCGCT": 1097439,
            "GCGCC": 1571570,
            "GCGCG": 782708,
            "GCGGA": 1115591,
            "GCGGT": 655563,
            "GCGGC": 2171550,
            "GCGGG": 1431138,
            "GGAAA": 2410741,
            "GGAAT": 1235110,
            "GGAAC": 1574504,
            "GGAAG": 3057526,
            "GGATA": 673349,
            "GGATT": 1044182,
            "GGATC": 1177606,
            "GGATG": 2160060,
            "GGACA": 2011449,
            "GGACT": 1438855,
            "GGACC": 1969621,
            "GGACG": 1066659,
            "GGAGA": 3277230,
            "GGAGT": 1486944,
            "GGAGC": 3202520,
            "GGAGG": 3444557,
            "GGTAA": 515887,
            "GGTAT": 565763,
            "GGTAC": 634973,
            "GGTAG": 485789,
            "GGTTA": 459260,
            "GGTTT": 877588,
            "GGTTC": 975884,
            "GGTTG": 859939,
            "GGTCA": 1220992,
            "GGTCT": 996468,
            "GGTCC": 1194963,
            "GGTCG": 488687,
            "GGTGA": 1768633,
            "GGTGT": 1339146,
            "GGTGC": 1773579,
            "GGTGG": 2670888,
            "GGCAA": 1794366,
            "GGCAT": 1433878,
            "GGCAC": 1481068,
            "GGCAG": 2812407,
            "GGCTA": 877398,
            "GGCTT": 1446338,
            "GGCTC": 1964504,
            "GGCTG": 3199551,
            "GGCCA": 2907212,
            "GGCCT": 2267888,
            "GGCCC": 2870135,
            "GGCCG": 2006737,
            "GGCGA": 820477,
            "GGCGT": 693765,
            "GGCGC": 1363258,
            "GGCGG": 1820754,
            "GGGAA": 1759714,
            "GGGAT": 976998,
            "GGGAC": 1477479,
            "GGGAG": 2052719,
            "GGGTA": 455842,
            "GGGTT": 604857,
            "GGGTC": 971169,
            "GGGTG": 1342528,
            "GGGCA": 1898589,
            "GGGCT": 1836967,
            "GGGCC": 2432546,
            "GGGCG": 1160416,
            "GGGGA": 1598665,
            "GGGGT": 834802,
            "GGGGC": 1886272,
            "GGGGG": 5242768
          },
          "overrepresented_sequences": {}
        },
        "command": "fastp --in1 rna_s1200_S21_1.fastq.gz --in2 rna_s1200_S21_2.fastq.gz --out1 rna_s1200_S21_1.fastp.fastq.gz --out2 rna_s1200_S21_2.fastp.fastq.gz --json rna_s1200_S21.fastp.json --html rna_s1200_S21.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 10000000 "
      }
    },
    "multiqc_fastqc": {
      "rna_s1221_S42_trimmed_1": {
        "Filename": "rna_s1221_S42_trimmed_1.gz",
        "File type": "Conventional base calls",
        "Encoding": "Sanger / Illumina 1.9",
        "Total Sequences": 9932006.0,
        "Total Bases": "1.3 Gbp",
        "Sequences flagged as poor quality": 0.0,
        "Sequence length": "15-150",
        "%GC": 53.0,
        "total_deduplicated_percentage": 41.93373866330315,
        "avg_sequence_length": 138.4228959386452,
        "median_sequence_length": 150,
        "basic_statistics": "pass",
        "per_base_sequence_quality": "pass",
        "per_tile_sequence_quality": "pass",
        "per_sequence_quality_scores": "pass",
        "per_base_sequence_content": "fail",
        "per_sequence_gc_content": "pass",
        "per_base_n_content": "pass",
        "sequence_length_distribution": "warn",
        "sequence_duplication_levels": "fail",
        "overrepresented_sequences": "pass",
        "adapter_content": "pass"
      },
      "rna_s1200_S21_trimmed_2": {
        "Filename": "rna_s1200_S21_trimmed_2.gz",
        "File type": "Conventional base calls",
        "Encoding": "Sanger / Illumina 1.9",
        "Total Sequences": 9937706.0,
        "Total Bases": "1.3 Gbp",
        "Sequences flagged as poor quality": 0.0,
        "Sequence length": "16-150",
        "%GC": 53.0,
        "total_deduplicated_percentage": 48.56671955724535,
        "avg_sequence_length": 140.53251514987463,
        "median_sequence_length": 150,
        "basic_statistics": "pass",
        "per_base_sequence_quality": "pass",
        "per_tile_sequence_quality": "pass",
        "per_sequence_quality_scores": "pass",
        "per_base_sequence_content": "warn",
        "per_sequence_gc_content": "pass",
        "per_base_n_content": "pass",
        "sequence_length_distribution": "warn",
        "sequence_duplication_levels": "fail",
        "overrepresented_sequences": "warn",
        "adapter_content": "pass"
      },
      "rna_s1221_S42_1": {
        "Filename": "rna_s1221_S42_1.gz",
        "File type": "Conventional base calls",
        "Encoding": "Sanger / Illumina 1.9",
        "Total Sequences": 10000000.0,
        "Total Bases": "1.5 Gbp",
        "Sequences flagged as poor quality": 0.0,
        "Sequence length": 150.0,
        "%GC": 52.0,
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  "config_creation_date": "2026-06-02, 20:58 UTC",
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  "config_report_comment": "This report has been generated by the <a href=\"https://github.com/nf-core/rnafusion/releases/tag/3.0.2\" target=\"_blank\">nf-core/rnafusion</a> analysis pipeline. For information about how to interpret these results, please see the <a href=\"https://nf-co.re/rnafusion/3.0.2/docs/output\" target=\"_blank\">documentation</a>.\n",
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