Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/28/35a2b6edd6a663d929973d1de6ec7c/rna_s1200_S21_1.fastp.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/66c74e4be1d4add2dcccfef769c235/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/36/618c07de8c81974a532c7fc328e03e/kallisto Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/66c74e4be1d4add2dcccfef769c235/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/28/35a2b6edd6a663d929973d1de6ec7c/rna_s1200_S21_2.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 194,360 [index] number of k-mers: 112,035,259 [quant] running in paired-end mode [quant] will process pair 1: rna_s1200_S21_1.fastp.fastq.gz rna_s1200_S21_2.fastp.fastq.gz [quant] finding pseudoalignments for the reads ... [progress] 1M reads processed (97.9% mapped) [progress] 2M reads processed (97.8% mapped) [progress] 3M reads processed (97.8% mapped) [progress] 4M reads processed (97.8% mapped) [progress] 5M reads processed (97.8% mapped) [progress] 6M reads processed (97.8% mapped) [progress] 7M reads processed (97.8% mapped) [progress] 8M reads processed (97.8% mapped) [progress] 9M reads processed (97.8% mapped) done [quant] processed 9,937,706 reads, 9,721,684 reads pseudoaligned [quant] estimated average fragment length: 181.2 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,157 rounds