Done. -parsing GTF file: /tmp/nxf.tfHYSsibyR/rna_s1221_S42.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.tfHYSsibyR/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.tfHYSsibyR/rna_s1221_S42_1.fastp.fastq.gz /tmp/nxf.tfHYSsibyR/rna_s1221_S42_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam rna_s1221_S42.star.sortedByCoord.out.bam * Running CMD: samtools index rna_s1221_S42.star.sortedByCoord.out.bam 00:27:36 : INFO : Done. -parsing GTF file: /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.gtf -parsing /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] -done parsing /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { ' ** passed ** ' => 351, 'num_hits: 1 != num_counted_on_fusion_contigs 2 ' => 32, 'excessive soft clipping' => 503, 'low complexity anchor region' => 4, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 1, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 6, 'per_id < 96' => 142, 'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 2 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] -fusion SPANNING read extraction for scaff: AC015911.9--SLFN12L [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] -fusion SPANNING read extraction for scaff: CD74--ROS1 -fusion SPANNING read extraction for scaff: EGFR--SEPTIN14 [1000] [2000] -fusion SPANNING read extraction for scaff: ETV6--NTRK3 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: FGFR3--TACC3 -fusion SPANNING read extraction for scaff: FGFR3--BAIAP2L1 [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: KIF5B--RET [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: LMNA--NTRK1 [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: NCOA4--RET -fusion SPANNING read extraction for scaff: PAX8--PPARG [1000] [2000] -fusion SPANNING read extraction for scaff: SLC45A3--BRAF -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 317, 'seq similar region alignment' => 549 }; EM: Starting log likelihood: -478.690299 EM: Round [1] log likelihood: -478.690299 EM: Stopping iterations at round 1 due to insufficient improvement in likelihood. -extracting read coordinates from /tmp/nxf.tfHYSsibyR/rna_s1221_S42.consolidated.bam into /tmp/nxf.tfHYSsibyR/rna_s1221_S42.consolidated.bam.read_coords CMD: touch /tmp/nxf.tfHYSsibyR/rna_s1221_S42.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.tfHYSsibyR/rna_s1221_S42.consolidated.bam.read_coords > /tmp/nxf.tfHYSsibyR/rna_s1221_S42.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.tfHYSsibyR/rna_s1221_S42.consolidated.bam.frag_coords > /tmp/nxf.tfHYSsibyR/rna_s1221_S42.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.tfHYSsibyR/fi_workdir/rna_s1221_S42.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.tfHYSsibyR/rna_s1221_S42.post_blast_and_promiscuity_filter -done, see /tmp/nxf.tfHYSsibyR/rna_s1221_S42.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -478.690299 EM: Round [1] log likelihood: -478.690299 EM: Stopping iterations at round 1 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.tfHYSsibyR/rna_s1221_S42_1.fastp.fastq.gz: 9932006 Warning - not locating file: /tmp/nxf.tfHYSsibyR/rna_s1221_S42.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000286030.2] WARNING, no entry stored in dbm for [ENSG00000286030.2] ls: cannot access 'IGV_inputs': No such file or directory