Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 10000000 total bases: 1500000000 Q20 bases: 1488646967(99.2431%) Q30 bases: 1469875906(97.9917%) Read2 before filtering: total reads: 10000000 total bases: 1500000000 Q20 bases: 1484512451(98.9675%) Q30 bases: 1458809565(97.254%) Read1 after filtering: total reads: 9937706 total bases: 1396572795 Q20 bases: 1391108252(99.6087%) Q30 bases: 1377664520(98.6461%) Read2 after filtering: total reads: 9937706 total bases: 1396684695 Q20 bases: 1389391946(99.4779%) Q30 bases: 1372670092(98.2806%) Filtering result: reads passed filter: 19875412 reads failed due to low quality: 124550 reads failed due to too many N: 0 reads failed due to too short: 38 reads with adapter trimmed: 6547787 bases trimmed due to adapters: 188610493 Duplication rate: 13.3191% Insert size peak (evaluated by paired-end reads): 139 JSON report: rna_s1200_S21.fastp.json HTML report: rna_s1200_S21.fastp.html fastp --in1 rna_s1200_S21_1.fastq.gz --in2 rna_s1200_S21_2.fastq.gz --out1 rna_s1200_S21_1.fastp.fastq.gz --out2 rna_s1200_S21_2.fastp.fastq.gz --json rna_s1200_S21.fastp.json --html rna_s1200_S21.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 10000000 fastp v0.23.4, time used: 22 seconds