Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b6/76237e13b680c7e412ae0fad56c0d4/rna_s1200_S21_1.fastp.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/9e4a1c25b25949798a990581a234ad/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bf/882aadc99cc8e547c6c8b05addb588/kallisto Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/9e4a1c25b25949798a990581a234ad/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b6/76237e13b680c7e412ae0fad56c0d4/rna_s1200_S21_2.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 194,360 [index] number of k-mers: 112,035,259 [quant] running in paired-end mode [quant] will process pair 1: rna_s1200_S21_1.fastp.fastq.gz rna_s1200_S21_2.fastp.fastq.gz [quant] finding pseudoalignments for the reads ... [progress] 1M reads processed (97.9% mapped) [progress] 2M reads processed (97.8% mapped) [progress] 3M reads processed (97.8% mapped) [progress] 4M reads processed (97.8% mapped) [progress] 5M reads processed (97.8% mapped) [progress] 6M reads processed (97.8% mapped) [progress] 7M reads processed (97.8% mapped) [progress] 8M reads processed (97.8% mapped) [progress] 9M reads processed (97.8% mapped) done [quant] processed 9,937,706 reads, 9,721,684 reads pseudoaligned [quant] estimated average fragment length: 181.2 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,157 rounds