Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/75/9c65993edb9600a6a7c3dcc543c185/rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/48/1933067be7a984fba87bcade064591/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/47/b7f3cf478bfcd8b88eb658343c676b/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/47/b7f3cf478bfcd8b88eb658343c676b/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/53/dee80bb1b28d6b5f14532311216acf/rna_s1221_S42.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (560870) splicing reads. 93891 splicing junctions. 89612 known splicing junctions. 4279 novel splicing junctions.
sampling 10% (1121740) splicing reads. 112775 splicing junctions. 104850 known splicing junctions. 7925 novel splicing junctions.
sampling 15% (1682611) splicing reads. 123669 splicing junctions. 112534 known splicing junctions. 11135 novel splicing junctions.
sampling 20% (2243481) splicing reads. 131447 splicing junctions. 117495 known splicing junctions. 13952 novel splicing junctions.
sampling 25% (2804352) splicing reads. 137576 splicing junctions. 121127 known splicing junctions. 16449 novel splicing junctions.
sampling 30% (3365222) splicing reads. 142606 splicing junctions. 123901 known splicing junctions. 18705 novel splicing junctions.
sampling 35% (3926092) splicing reads. 146965 splicing junctions. 126176 known splicing junctions. 20789 novel splicing junctions.
sampling 40% (4486963) splicing reads. 150821 splicing junctions. 128088 known splicing junctions. 22733 novel splicing junctions.
sampling 45% (5047833) splicing reads. 154270 splicing junctions. 129765 known splicing junctions. 24505 novel splicing junctions.
sampling 50% (5608704) splicing reads. 157379 splicing junctions. 131238 known splicing junctions. 26141 novel splicing junctions.
sampling 55% (6169574) splicing reads. 160168 splicing junctions. 132481 known splicing junctions. 27687 novel splicing junctions.
sampling 60% (6730444) splicing reads. 162633 splicing junctions. 133526 known splicing junctions. 29107 novel splicing junctions.
sampling 65% (7291315) splicing reads. 164979 splicing junctions. 134519 known splicing junctions. 30460 novel splicing junctions.
sampling 70% (7852185) splicing reads. 166966 splicing junctions. 135405 known splicing junctions. 31561 novel splicing junctions.
sampling 75% (8413056) splicing reads. 168767 splicing junctions. 136161 known splicing junctions. 32606 novel splicing junctions.
sampling 80% (8973926) splicing reads. 170473 splicing junctions. 136874 known splicing junctions. 33599 novel splicing junctions.
sampling 85% (9534796) splicing reads. 172029 splicing junctions. 137515 known splicing junctions. 34514 novel splicing junctions.
sampling 90% (10095667) splicing reads. 173413 splicing junctions. 138083 known splicing junctions. 35330 novel splicing junctions.
sampling 95% (10656537) splicing reads. 174672 splicing junctions. 138556 known splicing junctions. 36116 novel splicing junctions.
sampling 100% (11217408) splicing reads. 175815 splicing junctions. 139001 known splicing junctions. 36814 novel splicing junctions.
null device 
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