.command.log - Workdir - NFCORE_SAREK:SAREK:CRAM_SAMPLE_INTEGRITY:BAM_CONTAMINATION_ANALYSIS:CONPAIR_PILEUP (Sig_18_tissue)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/46/35defab8e223aa1554b5b02bd42187/.command.log
Size: 5.0 KB
Task: NFCORE_SAREK:SAREK:CRAM_SAMPLE_INTEGRITY:BAM_CONTAMINATION_ANALYSIS:CONPAIR_PILEUP (Sig_18_tissue)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/46/35defab8e223aa1554b5b02bd42187/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-inputs/test_sarek/end_to_end_regression/01_preprocess_regression_fastqs/regression_preprocess__60492e9--20260310-034416/alignments/recalibrated/Sig_18_tissue/Sig_18_tissue.recalibrated.bam
  Downloading: s3://natera-platform-sandbox/pipeline-inputs/test_sarek/end_to_end_regression/01_preprocess_regression_fastqs/regression_preprocess__60492e9--20260310-034416/alignments/recalibrated/Sig_18_tissue/Sig_18_tissue.recalibrated.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/46/35defab8e223aa1554b5b02bd42187/.command.run
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.dict
==> STAGING COMPLETE (7 inputs)

22:21:07.803 WARN  GATKReadFilterPluginDescriptor - Redundant enabled filter (NotDuplicateReadFilter) is enabled for this tool by default
22:21:07.862 INFO  NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/opt/conda/envs/conpair/share/gatk4-4.6.2.0-0/gatk-package-4.6.2.0-local.jar!/com/intel/gkl/native/libgkl_compression.so
22:21:07.973 INFO  Pileup - ------------------------------------------------------------
22:21:07.975 INFO  Pileup - The Genome Analysis Toolkit (GATK) v4.6.2.0
22:21:07.976 INFO  Pileup - For support and documentation go to https://software.broadinstitute.org/gatk/
22:21:07.976 INFO  Pileup - Executing as ?@nf-4635defab8e223aa1554b5b02bd42187-7971a-j4fqk on Linux v6.12.83 amd64
22:21:07.976 INFO  Pileup - Java runtime: OpenJDK 64-Bit Server VM v17.0.14+1-b1367.22
22:21:07.976 INFO  Pileup - Start Date/Time: May 28, 2026 at 10:21:07 PM UTC
22:21:07.976 INFO  Pileup - ------------------------------------------------------------
22:21:07.976 INFO  Pileup - ------------------------------------------------------------
22:21:07.977 INFO  Pileup - HTSJDK Version: 4.2.0
22:21:07.977 INFO  Pileup - Picard Version: 3.4.0
22:21:07.977 INFO  Pileup - Built for Spark Version: 3.5.0
22:21:07.979 INFO  Pileup - HTSJDK Defaults.COMPRESSION_LEVEL : 2
22:21:07.980 INFO  Pileup - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false
22:21:07.980 INFO  Pileup - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true
22:21:07.980 INFO  Pileup - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false
22:21:07.980 INFO  Pileup - Deflater: IntelDeflater
22:21:07.980 INFO  Pileup - Inflater: IntelInflater
22:21:07.980 INFO  Pileup - GCS max retries/reopens: 20
22:21:07.981 INFO  Pileup - Requester pays: disabled
22:21:07.981 INFO  Pileup - Initializing engine
22:21:08.463 INFO  FeatureManager - Using codec BEDCodec to read file file:///opt/conpair/conpair/markers/GRCh38.autosomes.phase3_shapeit2_mvncall_integrated.20130502.SNV.genotype.sselect_v4_MAF_0.4_LD_0.8.liftover.bed
22:21:08.513 INFO  IntervalArgumentCollection - Processing 7353 bp from intervals
22:21:08.531 INFO  Pileup - Done initializing engine
22:21:08.531 INFO  ProgressMeter - Starting traversal
22:21:08.532 INFO  ProgressMeter -        Current Locus  Elapsed Minutes        Loci Processed      Loci/Minute
22:21:12.809 INFO  Pileup - 0 read(s) filtered by: WellformedReadFilter 
0 read(s) filtered by: MappedReadFilter 
30096 read(s) filtered by: NotDuplicateReadFilter 
0 read(s) filtered by: PassesVendorQualityCheckReadFilter 
0 read(s) filtered by: NotSecondaryAlignmentReadFilter 
0 read(s) filtered by: CigarContainsNoNOperator 
0 read(s) filtered by: MatchingBasesAndQualsReadFilter 
30096 total reads filtered out of 105155 reads processed
22:21:12.809 INFO  ProgressMeter -             unmapped              0.1                   100           1402.9
22:21:12.809 INFO  ProgressMeter - Traversal complete. Processed 100 total loci in 0.1 minutes.
22:21:12.809 INFO  Pileup - Shutting down engine
[May 28, 2026 at 10:21:12 PM UTC] org.broadinstitute.hellbender.tools.walkers.qc.Pileup done. Elapsed time: 0.08 minutes.
Runtime.totalMemory()=394264576
Using GATK jar /opt/conda/envs/conpair/share/gatk4-4.6.2.0-0/gatk-package-4.6.2.0-local.jar
Running:
    java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -Xmx12g -jar /opt/conda/envs/conpair/share/gatk4-4.6.2.0-0/gatk-package-4.6.2.0-local.jar Pileup -R Homo_sapiens_assembly38.fasta -I Sig_18_tissue.recalibrated.bam -L /opt/conpair/conpair/markers/GRCh38.autosomes.phase3_shapeit2_mvncall_integrated.20130502.SNV.genotype.sselect_v4_MAF_0.4_LD_0.8.liftover.bed -O Sig_18_tissue.pileup.txt -verbose -RF NotDuplicateReadFilter -RF CigarContainsNoNOperator -RF MatchingBasesAndQualsReadFilter