Downloading: s3://natera-rnd-fsdx-dev-nextflow-scratch-01/work/ec/d673ee2ab685546e417b2c4a0238d7/.command.sh Downloading: s3://natera-rnd-fsdx-dev-nextflow-scratch-01/work/ec/d673ee2ab685546e417b2c4a0238d7/.command.run Downloading: s3://fsdx-algo-644019535899-us-west-2-an/aschultz/bf-gui-test/nf_gui_test_three.read1.fastq.gz Downloading: s3://fsdx-algo-644019535899-us-west-2-an/aschultz/bf-gui-test/nf_gui_test_three.read2.fastq.gz ==> STAGING COMPLETE (4 inputs) Read1 before filtering: total reads: 873162 total bases: 131847462 Q20 bases: 128636622(97.5647%) Q30 bases: 123346744(93.5526%) Q40 bases: 0(0%) Read2 before filtering: total reads: 873162 total bases: 131847462 Q20 bases: 128634525(97.5631%) Q30 bases: 123347261(93.553%) Q40 bases: 0(0%) Read1 after filtering: total reads: 871804 total bases: 114359507 Q20 bases: 111573988(97.5642%) Q30 bases: 106985631(93.552%) Q40 bases: 0(0%) Read2 after filtering: total reads: 871804 total bases: 114369597 Q20 bases: 111582137(97.5628%) Q30 bases: 106996183(93.553%) Q40 bases: 0(0%) Filtering result: reads passed filter: 1743608 reads failed due to low quality: 30 reads failed due to too many N: 0 reads failed due to too short: 2686 reads failed due to low complexity: 0 reads failed due to adapter dimer: 0 reads with adapter trimmed: 91176 bases trimmed due to adapters: 368196 Duplication rate: 74.1957% Insert size peak (evaluated by paired-end reads): 151 JSON report: nf_gui_test_three.fastp.json HTML report: nf_gui_test_three.fastp.html fastp -w 4 -i nf_gui_test_three.read1.fastq.gz -I nf_gui_test_three.read2.fastq.gz -o nf_gui_test_three.1.fastp.fq.gz -O nf_gui_test_three.2.fastp.fq.gz -b 141 -B 141 -g -y --html nf_gui_test_three.fastp.html --json nf_gui_test_three.fastp.json fastp v1.3.3, time used: 5 seconds