Downloading: s3://fsdx-algo-644019535899-us-west-2-an/aschultz/bf-gui-test/nf_gui_test_four.read2.fastq.gz Downloading: s3://natera-rnd-fsdx-dev-nextflow-scratch-01/work/d3/f6ee0ce994750a338381ae6c6c8240/.command.sh Downloading: s3://natera-rnd-fsdx-dev-nextflow-scratch-01/work/d3/f6ee0ce994750a338381ae6c6c8240/.command.run Downloading: s3://fsdx-algo-644019535899-us-west-2-an/aschultz/bf-gui-test/nf_gui_test_four.read1.fastq.gz ==> STAGING COMPLETE (4 inputs) Read1 before filtering: total reads: 877481 total bases: 132499631 Q20 bases: 129271711(97.5638%) Q30 bases: 123954128(93.5505%) Q40 bases: 0(0%) Read2 before filtering: total reads: 877481 total bases: 132499631 Q20 bases: 129271688(97.5638%) Q30 bases: 123960274(93.5552%) Q40 bases: 0(0%) Read1 after filtering: total reads: 875971 total bases: 114861617 Q20 bases: 112062866(97.5634%) Q30 bases: 107453173(93.5501%) Q40 bases: 0(0%) Read2 after filtering: total reads: 875971 total bases: 114856654 Q20 bases: 112058635(97.5639%) Q30 bases: 107454360(93.5552%) Q40 bases: 0(0%) Filtering result: reads passed filter: 1751942 reads failed due to low quality: 26 reads failed due to too many N: 0 reads failed due to too short: 2984 reads failed due to low complexity: 10 reads failed due to adapter dimer: 0 reads with adapter trimmed: 90180 bases trimmed due to adapters: 366560 Duplication rate: 74.3418% Insert size peak (evaluated by paired-end reads): 151 JSON report: nf_gui_test_four.fastp.json HTML report: nf_gui_test_four.fastp.html fastp -w 4 -i nf_gui_test_four.read1.fastq.gz -I nf_gui_test_four.read2.fastq.gz -o nf_gui_test_four.1.fastp.fq.gz -O nf_gui_test_four.2.fastp.fq.gz -b 141 -B 141 -g -y --html nf_gui_test_four.fastp.html --json nf_gui_test_four.fastp.json fastp v1.3.3, time used: 5 seconds