Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/stage-4eb95eab-fae6-42b1-9e7f-76da3ab8c895/a5/03c67a517d495c643c540de18d3053/FINAL_hotspot_list_07302025_hg38_sorted_w_anno_mods.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fc/b87092e57cce3ec62f29dce8c36922/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fc/b87092e57cce3ec62f29dce8c36922/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/11/afa9ce6becde4e811ea038189250d2/1136_7OO-T1-TDNA-1_A23WGJTLT4_2_vs_1136_7OO-N1-BDNA-1_A237CFYLT3_1.tnseq.hmnz.vcf.gz
==> STAGING COMPLETE (4 inputs)

Loaded 73 hotspot regions from FINAL_hotspot_list_07302025_hg38_sorted_w_anno_mods.bed
Detected annotation format: CSQ
Filtered: 476 PASS variants → 1 hotspot variants (1 unique)