Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/stage-4eb95eab-fae6-42b1-9e7f-76da3ab8c895/a5/03c67a517d495c643c540de18d3053/FINAL_hotspot_list_07302025_hg38_sorted_w_anno_mods.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/30213bfcbf7cf04be438846cb1592d/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/64/570bb2ff115e0db512591ae470270e/1136_814-T1-TDNA-1_A23WGJTLT4_1_vs_1136_814-N1-BDNA-1_A237CFYLT3_1.tnseq.hmnz.vcf.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/30213bfcbf7cf04be438846cb1592d/.command.run
==> STAGING COMPLETE (4 inputs)

Loaded 73 hotspot regions from FINAL_hotspot_list_07302025_hg38_sorted_w_anno_mods.bed
Detected annotation format: CSQ
Filtered: 2271 PASS variants → 2 hotspot variants (2 unique)