Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b1/8508a22065357104cdc9cfccf72e90/1029_BTOJ-T1-TDNA-1_A23WJ5GLT4_10_vs_1029_BTOJ-N1-BDNA-1_B23KGCJLT4_1.tnseq.hmnz.vcf.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/stage-02a7a0a2-75c7-4a9f-81d6-141deaf746e1/05/276485ce98c8d6e32e900e5b62d456/FINAL_hotspot_list_07302025_hg38_sorted_w_anno_mods.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/94/9cd9d6af4312efa6af411384ce10b5/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/94/9cd9d6af4312efa6af411384ce10b5/.command.run
==> STAGING COMPLETE (4 inputs)

Loaded 73 hotspot regions from FINAL_hotspot_list_07302025_hg38_sorted_w_anno_mods.bed
Detected annotation format: CSQ
Filtered: 2319 PASS variants → 1 hotspot variants (1 unique)