Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cb/e886ff04c1d5b7232fcf1cd7f70177/1029_16G-N1-BDNA-1_B23KGCJLT4_1_probes.tsv.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1b/e31bb91d03e8f78e9f62fb045578f4/.command.sh
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  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/tmp/66/18e293ffbf168b3f54b9c3529a9e52/manifest.tsv
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  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/78/45ae02c5431dfbd14485f5c9809da6/1029_0I-N1-BDNA-1_B23KGCJLT4_1_probes.tsv.gz
==> STAGING COMPLETE (8 inputs)

Blood manifest: 5 samples
Loaded 2,361,930 rows from 5 blood samples in 3.1s
GC reference: 472,386 probes
  Sex chrom homology mask: 1494 probes
  Pass 1: 4-feat blood GAM (16 workers)...
    5/5 blood samples, 12s, R²=0.4687
    Sex: 1 female, 4 male, 0 unknown
/usr/local/bin/build_norm_reference.py:247: RuntimeWarning: All-NaN slice encountered
  probe_coverage_median = np.nanmedian(blood_matrix, axis=1)
/usr/local/lib/python3.12/site-packages/numpy/lib/_nanfunctions_impl.py:1593: RuntimeWarning: All-NaN slice encountered
  return fnb._ureduce(a,
    Probe coverage: 471,724/472,386 valid probes
  Preliminary 5-feat GAM on blood (16 workers)...
    5/5 blood prelim 5-feat, 16s, R²=0.9495
/usr/local/bin/build_norm_reference.py:335: RuntimeWarning: All-NaN slice encountered
  prelim_median = np.nanmedian(prelim_matrix, axis=1)
/usr/local/lib/python3.12/site-packages/numpy/lib/_nanfunctions_impl.py:1593: RuntimeWarning: All-NaN slice encountered
  return fnb._ureduce(a,
    Probe mask: 35,837 masked (7.6%) — 13,635 low cov, 22,976 high var, 10,112 extreme
Wrote batch_reference.parquet (472,386 probes) to norm_reference/batch_reference.parquet
Wrote sex_calls.csv (5 samples) to norm_reference/sex_calls.csv
Wrote feature_stats.csv (5 samples) to norm_reference/feature_stats.csv
Build reference complete in 81.9s total