This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o SH032_HG514.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2747.950 CPU sec, 109.608 real sec [M::process] read 728278 sequences (100000020 bp) in 1.528 CPU sec, 1.533 real sec... [M::process] read 727474 sequences (100000037 bp) in 1.693 CPU sec, 1.744 real sec... [M::process] read 726844 sequences (100000229 bp) in 1.957 CPU sec, 2.016 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307141, 5, 19) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (276.71, 142.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 160, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (202.06, 123.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RR [M::process] read 726648 sequences (100000080 bp) in 1.829 CPU sec, 1.850 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306912, 3, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 217, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (201.75, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (281.35, 144.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 219, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (251.09, 116.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306752, 7, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 234, 3916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11460) [M::mem_pestat] mean and std.dev: (1050.08, 1570.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (280.07, 143.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 173, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (73, 268) [M::mem_pestat] mean and std.dev: (166.91, 22.32) [M::mem_pestat] low and high boundaries for proper pairs: (34, 307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728278 reads in 359.638 CPU sec, 11.973 real sec [M::mem_process_seqs] Processed 726844 reads in 220.659 CPU sec, 7.403 real sec [M::mem_process_seqs] Processed 727474 reads in 233.401 CPU sec, 7.876 real sec sorting block 0, #reads = 1104435 mem = 536876833 peak = 580747264 [M::process] read 725988 sequences (100000092 bp) in 2.326 CPU sec, 2.497 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306920, 8, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 189, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (168.08, 45.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (282.05, 143.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 173, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (189.00, 69.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 725412 sequences (100000020 bp) in 2.938 CPU sec, 3.320 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306440, 9, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 236, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (74, 439) [M::mem_pestat] mean and std.dev: (234.55, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (284.12, 145.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (201.43, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726648 reads in 215.545 CPU sec, 6.962 real sec sorting block 1, #reads = 1104081 mem = 536880489 peak = 763199488 [M::mem_process_seqs] Processed 725988 reads in 126.554 CPU sec, 4.068 real sec [M::process] read 725636 sequences (100000094 bp) in 2.076 CPU sec, 2.175 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305935, 7, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 210, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (227.44, 95.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (285.96, 146.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 226, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (221.70, 62.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 2, #reads = 1103384 mem = 536878831 peak = 763199488 [M::process] read 725614 sequences (100000242 bp) in 2.478 CPU sec, 2.647 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306042, 7, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 136, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (154.00, 77.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (286.13, 146.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 217, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (210.19, 64.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 725092 sequences (100000102 bp) in 1.803 CPU sec, 1.840 real sec... [M::mem_process_seqs] Processed 725412 reads in 213.914 CPU sec, 6.886 real sec [M::mem_process_seqs] Processed 725636 reads in 126.325 CPU sec, 4.068 real sec sorting block 3, #reads = 1102934 mem = 536878172 peak = 763199488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306150, 1, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 193, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (228.50, 99.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (285.23, 145.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 218, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (202.94, 55.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 724864 sequences (100000129 bp) in 2.166 CPU sec, 2.414 real sec... [M::process] read 724860 sequences (100000085 bp) in 1.602 CPU sec, 1.636 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304900, 8, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 227, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3110) [M::mem_pestat] mean and std.dev: (436.50, 402.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (286.97, 146.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 163, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (164.22, 63.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725614 reads in 213.303 CPU sec, 6.918 real sec [M::mem_process_seqs] Processed 725092 reads in 125.571 CPU sec, 4.056 real sec sorting block 4, #reads = 1102901 mem = 536877893 peak = 763199488 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305546, 4, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 252, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 433) [M::mem_pestat] mean and std.dev: (237.79, 51.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (289.18, 148.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 205, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (213.81, 90.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 724554 sequences (100000172 bp) in 2.111 CPU sec, 2.371 real sec... [M::process] read 724356 sequences (100000099 bp) in 1.737 CPU sec, 1.739 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305425, 4, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 164, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (182.46, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (289.25, 147.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 184, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (193.81, 75.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724864 reads in 208.937 CPU sec, 6.782 real sec sorting block 5, #reads = 1101876 mem = 536878300 peak = 774086656 [M::mem_process_seqs] Processed 724860 reads in 121.777 CPU sec, 3.925 real sec sorting block 6, #reads = 1102308 mem = 536879524 peak = 806858752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305815, 4, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 134, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (157.86, 52.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (290.73, 148.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 170, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (203.22, 151.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 723560 sequences (100000163 bp) in 2.196 CPU sec, 2.643 real sec... [M::process] read 724068 sequences (100000181 bp) in 1.676 CPU sec, 1.697 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305451, 1, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 174, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (187.00, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (289.77, 147.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (179.50, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724554 reads in 206.418 CPU sec, 6.799 real sec [M::mem_process_seqs] Processed 724356 reads in 119.072 CPU sec, 3.962 real sec sorting block 7, #reads = 1102066 mem = 536878577 peak = 806858752 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 304735, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (188.38, 66.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (292.78, 148.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 218, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (189.67, 64.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 724342 sequences (100000068 bp) in 2.800 CPU sec, 3.048 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305159, 7, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 207, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (218.28, 114.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (291.24, 147.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (216.06, 101.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723560 reads in 209.726 CPU sec, 6.759 real sec [M::process] read 726576 sequences (100000223 bp) in 1.918 CPU sec, 1.924 real sec... [M::mem_process_seqs] Processed 724068 reads in 123.327 CPU sec, 3.942 real sec sorting block 8, #reads = 1101159 mem = 536879911 peak = 817446912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305254, 8, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (218.89, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (290.58, 148.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (181.69, 51.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 727782 sequences (100000196 bp) in 2.551 CPU sec, 3.073 real sec... [M::process] read 727266 sequences (100000024 bp) in 1.625 CPU sec, 1.630 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306889, 6, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 139, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 212) [M::mem_pestat] mean and std.dev: (136.23, 35.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (282.39, 145.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 257, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (232.54, 96.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724342 reads in 209.218 CPU sec, 6.774 real sec sorting block 9, #reads = 1101204 mem = 536875589 peak = 817446912 [M::mem_process_seqs] Processed 726576 reads in 121.354 CPU sec, 3.899 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306993, 1, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (278.45, 143.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (260.89, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RR sorting block 10, #reads = 1102578 mem = 536880640 peak = 875134976 [M::process] read 726862 sequences (100000052 bp) in 2.883 CPU sec, 3.228 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306623, 1, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 184, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (201.92, 58.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (279.41, 142.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 155, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (203.23, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726730 sequences (100000062 bp) in 1.956 CPU sec, 1.964 real sec... [M::mem_process_seqs] Processed 727782 reads in 207.076 CPU sec, 6.996 real sec [M::mem_process_seqs] Processed 727266 reads in 119.689 CPU sec, 4.210 real sec sorting block 11, #reads = 1103961 mem = 536877687 peak = 875134976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306842, 7, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 164, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (195.53, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (280.00, 142.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 196, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (197.05, 72.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726046 sequences (100000135 bp) in 2.254 CPU sec, 2.450 real sec... [M::process] read 725994 sequences (100000092 bp) in 1.892 CPU sec, 1.896 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306850, 4, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 200, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (204.20, 89.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (281.55, 144.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 165, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (197.79, 99.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726862 reads in 202.211 CPU sec, 6.508 real sec [M::mem_process_seqs] Processed 726730 reads in 114.284 CPU sec, 3.650 real sec sorting block 12, #reads = 1104027 mem = 536879610 peak = 875134976 [M::process] read 725762 sequences (100000193 bp) in 2.654 CPU sec, 2.957 real sec... [M::process] read 725654 sequences (100000231 bp) in 1.782 CPU sec, 1.788 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306296, 2, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 184, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (199.46, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (283.03, 144.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 205, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (201.22, 57.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725994 reads in 108.590 CPU sec, 3.436 real sec sorting block 13, #reads = 1103790 mem = 536879440 peak = 875134976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306390, 4, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 184, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (175.08, 66.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (282.41, 144.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 196, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (205.45, 77.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306151, 4, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 169, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (193.00, 71.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (284.37, 144.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 236, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (281.59, 168.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 725866 sequences (100000177 bp) in 1.624 CPU sec, 1.672 real sec... [M::mem_process_seqs] Processed 726046 reads in 313.786 CPU sec, 10.062 real sec [M::mem_process_seqs] Processed 725762 reads in 127.640 CPU sec, 4.118 real sec sorting block 14, #reads = 1103225 mem = 536879769 peak = 875134976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306194, 4, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 190, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.08, 61.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (283.28, 143.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 137, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (188.41, 98.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 725218 sequences (100000221 bp) in 2.694 CPU sec, 3.063 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306108, 0, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 198, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (206.95, 74.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (284.19, 144.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 214, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (220.86, 109.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725654 reads in 205.706 CPU sec, 6.633 real sec [M::process] read 725112 sequences (100000216 bp) in 1.673 CPU sec, 1.680 real sec... [M::mem_process_seqs] Processed 725866 reads in 118.273 CPU sec, 3.765 real sec sorting block 15, #reads = 1102809 mem = 536879139 peak = 875134976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305600, 4, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 188, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (191.15, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (285.83, 145.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 175, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (199.40, 96.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 16, #reads = 1102958 mem = 536880229 peak = 875134976 [M::process] read 725010 sequences (100000193 bp) in 2.556 CPU sec, 2.828 real sec... [M::process] read 724788 sequences (100000109 bp) in 1.792 CPU sec, 1.800 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 306056, 7, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 215, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (200.70, 72.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (285.78, 145.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 239, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2858) [M::mem_pestat] mean and std.dev: (284.21, 235.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725218 reads in 206.304 CPU sec, 6.653 real sec [M::mem_process_seqs] Processed 725112 reads in 117.828 CPU sec, 3.782 real sec sorting block 17, #reads = 1102520 mem = 536881988 peak = 875134976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305733, 4, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 224, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (215.00, 95.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 265, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (288.50, 147.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::process] read 724404 sequences (100000152 bp) in 2.453 CPU sec, 2.673 real sec... [M::process] read 723550 sequences (100000111 bp) in 1.830 CPU sec, 1.837 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305852, 5, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344) [M::mem_pestat] mean and std.dev: (352.06, 303.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (289.84, 148.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 239, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (223.80, 99.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725010 reads in 205.148 CPU sec, 6.587 real sec [M::mem_process_seqs] Processed 724788 reads in 117.328 CPU sec, 3.763 real sec sorting block 18, #reads = 1102636 mem = 536879089 peak = 875134976 [M::process] read 723438 sequences (100000093 bp) in 3.078 CPU sec, 3.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304987, 4, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 200, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2638) [M::mem_pestat] mean and std.dev: (364.31, 342.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (292.08, 147.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 265, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (223.50, 87.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 723550 reads in 108.034 CPU sec, 3.420 real sec sorting block 19, #reads = 1101939 mem = 536880363 peak = 875134976 [M::process] read 726248 sequences (100000190 bp) in 2.933 CPU sec, 3.159 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 305900, 9, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 204, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (247.88, 114.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (290.27, 147.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 251, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (300.15, 196.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305250, 7, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 223, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (229.00, 87.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (294.58, 149.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 287, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (270.72, 116.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724404 reads in 310.631 CPU sec, 10.008 real sec [M::mem_process_seqs] Processed 723438 reads in 127.584 CPU sec, 4.108 real sec [M::process] read 727318 sequences (100000120 bp) in 2.713 CPU sec, 2.982 real sec... sorting block 20, #reads = 1101203 mem = 536877678 peak = 875134976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306137, 2, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 256, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (219.00, 68.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (284.23, 146.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (115, 189, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (234.23, 171.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306953, 3, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 229, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (183.40, 75.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (280.04, 143.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 187, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3045) [M::mem_pestat] mean and std.dev: (439.69, 495.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726248 reads in 206.893 CPU sec, 6.707 real sec [M::mem_process_seqs] Processed 727318 reads in 119.457 CPU sec, 3.837 real sec [M::process] read 727840 sequences (100000265 bp) in 3.749 CPU sec, 4.187 real sec... sorting block 21, #reads = 1101184 mem = 536881244 peak = 875134976 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306884, 4, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (213.39, 75.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (277.62, 142.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 159, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (148.60, 41.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 727544 sequences (100000213 bp) in 3.265 CPU sec, 3.654 real sec... [M::mem_process_seqs] Processed 727840 reads in 109.479 CPU sec, 3.689 real sec sorting block 22, #reads = 1103213 mem = 536877438 peak = 875134976 [M::process] read 727352 sequences (100000133 bp) in 2.698 CPU sec, 2.933 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306691, 2, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 301, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532) [M::mem_pestat] mean and std.dev: (438.38, 325.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (277.12, 141.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 177, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (184.53, 57.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726750 sequences (100000104 bp) in 1.933 CPU sec, 2.070 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306499, 5, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (165.62, 56.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 255, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (279.28, 143.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 238, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (215.33, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727544 reads in 208.929 CPU sec, 6.739 real sec [M::process] read 726840 sequences (100000070 bp) in 1.738 CPU sec, 1.762 real sec... [M::mem_process_seqs] Processed 727352 reads in 117.992 CPU sec, 3.748 real sec sorting block 23, #reads = 1104552 mem = 536879379 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306458, 2, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 201, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 320) [M::mem_pestat] mean and std.dev: (191.43, 52.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (279.71, 142.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 270, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (264.44, 112.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 24, #reads = 1104146 mem = 536878742 peak = 901095424 [M::process] read 727064 sequences (100000260 bp) in 3.032 CPU sec, 3.349 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306567, 4, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 184, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (210.84, 105.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (280.43, 143.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (194.31, 73.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726750 reads in 204.267 CPU sec, 6.573 real sec [M::mem_process_seqs] Processed 726840 reads in 115.084 CPU sec, 3.649 real sec [M::process] read 727162 sequences (100000024 bp) in 2.060 CPU sec, 2.115 real sec... sorting block 25, #reads = 1103801 mem = 536879753 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306927, 0, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 226, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (264.89, 140.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (278.95, 142.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 188, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 367) [M::mem_pestat] mean and std.dev: (189.47, 57.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726578 sequences (100000050 bp) in 2.648 CPU sec, 2.963 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306976, 5, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 185, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.93, 65.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (278.67, 142.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 168, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (193.00, 90.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727064 reads in 201.161 CPU sec, 6.496 real sec [M::mem_process_seqs] Processed 727162 reads in 114.594 CPU sec, 3.655 real sec [M::process] read 726692 sequences (100000014 bp) in 2.157 CPU sec, 2.227 real sec... sorting block 26, #reads = 1103954 mem = 536879900 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307120, 3, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 220, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (228.46, 65.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (280.95, 143.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 192, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (177.16, 58.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726328 sequences (100000193 bp) in 2.765 CPU sec, 3.168 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306556, 8, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 187, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (179.88, 61.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (280.43, 143.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 726578 reads in 203.297 CPU sec, 6.567 real sec [M::mem_process_seqs] Processed 726692 reads in 117.419 CPU sec, 3.736 real sec sorting block 27, #reads = 1103857 mem = 536876859 peak = 901095424 [M::process] read 726458 sequences (100000152 bp) in 2.159 CPU sec, 2.263 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306375, 2, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 241, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (221.57, 65.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (281.65, 144.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 224, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (255.13, 127.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 28, #reads = 1103640 mem = 536877580 peak = 901095424 [M::process] read 725700 sequences (100000264 bp) in 3.067 CPU sec, 3.462 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306477, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (193.63, 66.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (282.80, 144.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 251, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (202.35, 85.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726328 reads in 205.029 CPU sec, 6.613 real sec [M::mem_process_seqs] Processed 726458 reads in 118.567 CPU sec, 3.772 real sec [M::process] read 724548 sequences (100000254 bp) in 2.546 CPU sec, 2.731 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306242, 5, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (188.33, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (284.64, 145.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 189, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (228.50, 117.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 29, #reads = 1103846 mem = 536877767 peak = 901095424 [M::process] read 724072 sequences (100000025 bp) in 2.771 CPU sec, 3.079 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305771, 12, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 236, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (316.52, 253.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (288.92, 146.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 18, 43) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 107) [M::mem_pestat] mean and std.dev: (24.73, 26.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 139) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 216, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (203.75, 60.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725700 reads in 210.823 CPU sec, 6.785 real sec [M::mem_process_seqs] Processed 724548 reads in 119.968 CPU sec, 3.806 real sec [M::process] read 724580 sequences (100000269 bp) in 2.649 CPU sec, 2.799 real sec... sorting block 30, #reads = 1102996 mem = 536880829 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305524, 3, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 188, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (193.60, 81.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (292.50, 148.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 308, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (268.24, 96.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726992 sequences (100000085 bp) in 3.122 CPU sec, 3.528 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305014, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 237, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (264.43, 154.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (291.38, 149.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (169.72, 50.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724072 reads in 196.136 CPU sec, 6.343 real sec sorting block 31, #reads = 1101964 mem = 536878020 peak = 901095424 [M::process] read 726958 sequences (100000050 bp) in 2.766 CPU sec, 2.974 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 307165, 7, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (154.39, 61.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (280.51, 144.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 191, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (187.73, 71.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726992 reads in 101.849 CPU sec, 3.261 real sec [M::process] read 727886 sequences (100000282 bp) in 2.927 CPU sec, 3.156 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306654, 7, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 143, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (154.50, 50.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (279.81, 142.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 173, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (174.42, 76.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 32, #reads = 1103147 mem = 536877835 peak = 901095424 [M::mem_process_seqs] Processed 724580 reads in 304.869 CPU sec, 9.818 real sec [M::mem_process_seqs] Processed 726958 reads in 108.326 CPU sec, 3.550 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307112, 1, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 234, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (241.62, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (278.77, 143.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 216, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (205.18, 54.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 727666 sequences (100000012 bp) in 2.979 CPU sec, 3.328 real sec... [M::mem_process_seqs] Processed 727886 reads in 99.039 CPU sec, 3.315 real sec sorting block 33, #reads = 1102300 mem = 536879838 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306866, 3, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 184, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (200.07, 75.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (279.11, 143.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 195, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (182.64, 44.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727666 reads in 97.567 CPU sec, 3.277 real sec [M::process] read 727842 sequences (100000056 bp) in 3.130 CPU sec, 3.484 real sec... sorting block 34, #reads = 1104297 mem = 536877229 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306438, 1, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 196, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (194.79, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (277.71, 142.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (202.15, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727842 reads in 95.769 CPU sec, 3.209 real sec [M::process] read 727898 sequences (100000173 bp) in 3.147 CPU sec, 3.480 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307279, 7, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 213, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (198.62, 100.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (278.39, 143.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 304, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526) [M::mem_pestat] mean and std.dev: (320.24, 222.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727898 reads in 98.747 CPU sec, 3.304 real sec [M::process] read 728114 sequences (100000134 bp) in 3.209 CPU sec, 3.721 real sec... sorting block 35, #reads = 1104356 mem = 536878687 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 307083, 5, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 193, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (259.25, 155.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (277.55, 142.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 238, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (245.15, 131.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728114 reads in 94.168 CPU sec, 3.160 real sec [M::process] read 728556 sequences (100000044 bp) in 3.127 CPU sec, 3.518 real sec... sorting block 36, #reads = 1104471 mem = 536877700 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 307495, 6, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 229, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (213.82, 67.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (275.95, 142.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 177, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 272) [M::mem_pestat] mean and std.dev: (162.90, 33.35) [M::mem_pestat] low and high boundaries for proper pairs: (29, 309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728556 reads in 92.888 CPU sec, 3.120 real sec [M::process] read 728542 sequences (100000038 bp) in 3.225 CPU sec, 3.580 real sec... sorting block 37, #reads = 1104811 mem = 536879322 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307400, 2, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 151, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (178.83, 86.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (274.47, 140.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 180, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (195.20, 66.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728542 reads in 97.491 CPU sec, 3.222 real sec [M::process] read 728486 sequences (100000060 bp) in 3.734 CPU sec, 4.179 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 307492, 7, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 169, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (157.08, 36.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 250, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (275.19, 141.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 218, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (225.69, 99.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728486 reads in 97.843 CPU sec, 3.223 real sec [M::process] read 727974 sequences (100000172 bp) in 3.218 CPU sec, 3.633 real sec... sorting block 38, #reads = 1104514 mem = 536879410 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 307200, 5, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 217, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (311.42, 245.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (275.81, 141.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 213, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (231.81, 70.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727974 reads in 98.718 CPU sec, 3.228 real sec [M::process] read 728136 sequences (100000186 bp) in 3.232 CPU sec, 3.523 real sec... sorting block 39, #reads = 1104903 mem = 536878137 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307277, 2, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 164, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (169.58, 47.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (276.39, 142.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 216, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 356) [M::mem_pestat] mean and std.dev: (203.20, 51.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728136 reads in 95.054 CPU sec, 3.100 real sec [M::process] read 727436 sequences (100000160 bp) in 3.381 CPU sec, 3.807 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306962, 3, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 171, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (179.07, 83.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (278.77, 143.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 197, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (201.63, 80.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727436 reads in 95.262 CPU sec, 3.104 real sec sorting block 40, #reads = 1104924 mem = 536877895 peak = 901095424 [M::process] read 727072 sequences (100000254 bp) in 3.372 CPU sec, 3.730 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306546, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 196, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.24, 64.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (280.60, 143.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 219, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (206.22, 63.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727072 reads in 95.317 CPU sec, 3.101 real sec [M::process] read 725464 sequences (100000149 bp) in 3.185 CPU sec, 3.769 real sec... sorting block 41, #reads = 1104535 mem = 536880525 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 305517, 5, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (286.06, 145.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 198, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (182.50, 72.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725464 reads in 98.156 CPU sec, 3.230 real sec [M::process] read 725812 sequences (100000068 bp) in 3.623 CPU sec, 4.105 real sec... sorting block 42, #reads = 1102256 mem = 536879850 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306050, 5, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 169, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (221.25, 102.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (286.11, 146.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 148, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (199.69, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725812 reads in 97.604 CPU sec, 3.240 real sec [M::process] read 725276 sequences (100000002 bp) in 2.931 CPU sec, 3.345 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306100, 0, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 210, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (202.91, 78.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (286.59, 146.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 242, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (210.10, 77.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725276 reads in 97.145 CPU sec, 3.161 real sec [M::process] read 725832 sequences (100000038 bp) in 3.889 CPU sec, 4.360 real sec... sorting block 43, #reads = 1102636 mem = 536878731 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305520, 4, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 162, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (169.31, 48.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (282.73, 144.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 254, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2721) [M::mem_pestat] mean and std.dev: (271.73, 240.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 727280 sequences (100000078 bp) in 2.682 CPU sec, 2.904 real sec... [M::mem_process_seqs] Processed 725832 reads in 97.873 CPU sec, 3.193 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306533, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 206, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (210.29, 99.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 256, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (280.92, 145.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 196, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 352) [M::mem_pestat] mean and std.dev: (172.41, 48.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727280 reads in 95.029 CPU sec, 3.106 real sec [M::process] read 728608 sequences (100000070 bp) in 3.508 CPU sec, 3.847 real sec... sorting block 44, #reads = 1102473 mem = 536877111 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307126, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 184, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (192.59, 68.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (279.01, 145.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 227, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (298.59, 237.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728608 reads in 98.852 CPU sec, 3.226 real sec [M::process] read 727676 sequences (100000162 bp) in 2.900 CPU sec, 3.343 real sec... sorting block 45, #reads = 1103959 mem = 536879528 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306865, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (151.27, 42.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 255, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (280.72, 145.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 158, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (187.58, 78.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727676 reads in 95.668 CPU sec, 3.139 real sec [M::process] read 727290 sequences (100000030 bp) in 3.332 CPU sec, 3.654 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306368, 3, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 254, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (310.61, 160.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (281.60, 144.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 219, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (244.29, 86.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727290 reads in 102.426 CPU sec, 3.456 real sec [M::process] read 726916 sequences (100000135 bp) in 3.699 CPU sec, 4.268 real sec... sorting block 46, #reads = 1104634 mem = 536879322 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306392, 2, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 195, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (239.65, 140.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (282.88, 145.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 188, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 297) [M::mem_pestat] mean and std.dev: (171.56, 46.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726916 reads in 99.649 CPU sec, 3.284 real sec [M::process] read 726502 sequences (100000168 bp) in 3.083 CPU sec, 3.326 real sec... sorting block 47, #reads = 1103458 mem = 536880295 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 305829, 5, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (190.64, 67.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (283.41, 145.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 226, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (254.15, 133.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726502 reads in 99.181 CPU sec, 3.240 real sec [M::process] read 726268 sequences (100000108 bp) in 3.043 CPU sec, 3.320 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305704, 4, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 277, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (241.71, 97.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 260, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (285.02, 146.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 204, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 455) [M::mem_pestat] mean and std.dev: (204.38, 69.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726268 reads in 99.866 CPU sec, 3.276 real sec [M::process] read 726150 sequences (100000153 bp) in 3.264 CPU sec, 3.574 real sec... sorting block 48, #reads = 1103312 mem = 536878474 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306044, 4, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 273, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (434.00, 387.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (285.50, 146.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 163, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (185.22, 85.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726150 reads in 99.781 CPU sec, 3.273 real sec [M::process] read 726084 sequences (100000222 bp) in 3.132 CPU sec, 3.406 real sec... sorting block 49, #reads = 1102859 mem = 536878988 peak = 901095424 [M::process] read 725920 sequences (100000127 bp) in 2.260 CPU sec, 2.505 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306156, 2, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 217, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 437) [M::mem_pestat] mean and std.dev: (206.19, 82.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (284.03, 145.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 167, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (211.73, 90.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 725450 sequences (100000134 bp) in 2.113 CPU sec, 2.227 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306704, 1, 19) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (286.46, 147.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 177, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (332.68, 321.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1737) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726084 reads in 186.505 CPU sec, 5.952 real sec [M::mem_process_seqs] Processed 725920 reads in 108.692 CPU sec, 3.457 real sec [M::process] read 725072 sequences (100000094 bp) in 1.866 CPU sec, 1.925 real sec... sorting block 50, #reads = 1103072 mem = 536878040 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305824, 7, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (185.50, 54.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (286.48, 146.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 188, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 343) [M::mem_pestat] mean and std.dev: (179.06, 42.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305141, 5, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 208, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (181.91, 47.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 265, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (288.37, 147.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 235, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (254.24, 102.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 724846 sequences (100000005 bp) in 3.381 CPU sec, 3.831 real sec... [M::mem_process_seqs] Processed 725450 reads in 181.557 CPU sec, 5.875 real sec [M::mem_process_seqs] Processed 725072 reads in 106.588 CPU sec, 3.402 real sec sorting block 51, #reads = 1102283 mem = 536877631 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305385, 8, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 152, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (189.43, 113.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (289.77, 148.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 208, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (217.22, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 724896 sequences (100000226 bp) in 3.167 CPU sec, 3.516 real sec... [M::mem_process_seqs] Processed 724846 reads in 97.812 CPU sec, 3.269 real sec sorting block 52, #reads = 1102438 mem = 536878035 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305215, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 214, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (189.21, 43.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (290.23, 148.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 306, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3833) [M::mem_pestat] mean and std.dev: (783.11, 832.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 724632 sequences (100000135 bp) in 2.825 CPU sec, 3.056 real sec... [M::mem_process_seqs] Processed 724896 reads in 100.195 CPU sec, 3.308 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305154, 5, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 255, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1323) [M::mem_pestat] mean and std.dev: (309.53, 192.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (291.84, 150.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 246, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (264.14, 91.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724632 reads in 97.949 CPU sec, 3.241 real sec [M::process] read 724504 sequences (100000030 bp) in 3.209 CPU sec, 3.660 real sec... sorting block 53, #reads = 1102034 mem = 536877586 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304929, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 276, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (244.21, 103.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.10, 151.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 206, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (210.28, 74.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724504 reads in 101.125 CPU sec, 3.341 real sec [M::process] read 726502 sequences (100000070 bp) in 3.083 CPU sec, 3.377 real sec... sorting block 54, #reads = 1101438 mem = 536877829 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306660, 3, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 159, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (172.79, 53.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 259, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (284.40, 147.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 208, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 366) [M::mem_pestat] mean and std.dev: (192.29, 47.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726502 reads in 98.445 CPU sec, 3.272 real sec [M::process] read 728520 sequences (100000232 bp) in 3.254 CPU sec, 3.567 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307147, 6, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 55, #reads = 1101899 mem = 536880476 peak = 901095424 [M::mem_pestat] (25, 50, 75) percentile: (164, 251, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (279.07, 146.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 190, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (170.93, 41.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728520 reads in 98.115 CPU sec, 3.246 real sec [M::process] read 728466 sequences (100000017 bp) in 3.242 CPU sec, 3.647 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307520, 3, 18) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 253, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (278.62, 144.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 215, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (222.73, 122.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728466 reads in 97.195 CPU sec, 3.213 real sec [M::process] read 727864 sequences (100000231 bp) in 3.073 CPU sec, 3.401 real sec... sorting block 56, #reads = 1104574 mem = 536878575 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306816, 2, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 218, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2903) [M::mem_pestat] mean and std.dev: (606.94, 561.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 256, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (281.00, 145.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 181, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (183.26, 55.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727864 reads in 99.433 CPU sec, 3.269 real sec [M::process] read 727146 sequences (100000141 bp) in 3.549 CPU sec, 3.935 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306251, 6, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 214, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (254.56, 129.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 256, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (280.37, 143.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 214, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (214.23, 91.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727146 reads in 100.592 CPU sec, 3.307 real sec sorting block 57, #reads = 1104937 mem = 536879894 peak = 901095424 [M::process] read 726844 sequences (100000189 bp) in 3.623 CPU sec, 4.086 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305961, 5, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 181, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (178.43, 48.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (280.91, 143.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 726844 reads in 96.760 CPU sec, 3.184 real sec [M::process] read 726706 sequences (100000248 bp) in 3.256 CPU sec, 3.552 real sec... sorting block 58, #reads = 1103210 mem = 536878566 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306310, 7, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 170, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (306.19, 301.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (282.20, 144.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (175.58, 65.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726706 reads in 96.917 CPU sec, 3.164 real sec [M::process] read 726354 sequences (100000213 bp) in 3.188 CPU sec, 3.511 real sec... sorting block 59, #reads = 1103128 mem = 536882914 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306133, 6, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 233, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931) [M::mem_pestat] mean and std.dev: (201.38, 88.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (283.50, 145.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 177, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (219.41, 89.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726354 reads in 97.771 CPU sec, 3.183 real sec [M::process] read 726334 sequences (100000074 bp) in 3.172 CPU sec, 3.543 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306463, 1, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 199, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (224.00, 96.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (282.71, 144.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (223.33, 76.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726334 reads in 97.165 CPU sec, 3.164 real sec [M::process] read 726046 sequences (100000170 bp) in 3.398 CPU sec, 3.767 real sec... sorting block 60, #reads = 1103543 mem = 536879902 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305922, 3, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (191.00, 70.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (283.83, 144.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 216, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 359) [M::mem_pestat] mean and std.dev: (200.79, 62.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726046 reads in 93.661 CPU sec, 3.046 real sec [M::process] read 725808 sequences (100000039 bp) in 3.263 CPU sec, 3.559 real sec... sorting block 61, #reads = 1102122 mem = 536876781 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306305, 6, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 228, 2220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6398) [M::mem_pestat] mean and std.dev: (891.94, 1358.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (285.31, 146.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 198, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (199.58, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 725900 sequences (100000036 bp) in 2.858 CPU sec, 3.208 real sec... [M::mem_process_seqs] Processed 725808 reads in 98.641 CPU sec, 3.213 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306182, 3, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (207.21, 102.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (285.20, 146.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 196, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (215.84, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725900 reads in 97.930 CPU sec, 3.191 real sec [M::process] read 725688 sequences (100000020 bp) in 2.998 CPU sec, 3.233 real sec... sorting block 62, #reads = 1102726 mem = 536879349 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305729, 4, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 198, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (181.50, 59.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (285.12, 145.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 195, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (201.57, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725688 reads in 95.223 CPU sec, 3.109 real sec [M::process] read 725354 sequences (100000228 bp) in 2.982 CPU sec, 3.301 real sec... sorting block 63, #reads = 1103176 mem = 536881299 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 305482, 8, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 156, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (168.71, 56.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (287.30, 147.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 206, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (353.29, 271.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725354 reads in 98.475 CPU sec, 3.192 real sec [M::process] read 724708 sequences (100000006 bp) in 3.336 CPU sec, 3.711 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305631, 3, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (163.27, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (289.80, 148.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 250, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (230.58, 82.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724708 reads in 99.790 CPU sec, 3.229 real sec [M::process] read 725250 sequences (100000271 bp) in 3.203 CPU sec, 3.474 real sec... sorting block 64, #reads = 1102222 mem = 536878354 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305495, 4, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 228, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (195.69, 66.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 266, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (290.50, 149.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 271, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (283.00, 152.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725250 reads in 98.799 CPU sec, 3.207 real sec [M::process] read 724904 sequences (100000058 bp) in 3.128 CPU sec, 3.424 real sec... sorting block 65, #reads = 1102327 mem = 536879514 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304959, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 221, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (218.10, 78.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (291.71, 150.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 217, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (253.23, 141.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724904 reads in 94.699 CPU sec, 3.069 real sec [M::process] read 727474 sequences (100000181 bp) in 2.836 CPU sec, 3.203 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306317, 5, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 210, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (222.50, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (281.29, 146.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 272, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (210.91, 113.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727474 reads in 97.313 CPU sec, 3.150 real sec sorting block 66, #reads = 1101370 mem = 536878600 peak = 901095424 [M::process] read 728526 sequences (100000132 bp) in 3.428 CPU sec, 3.675 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307125, 3, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 182, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (193.85, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 251, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (278.53, 146.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 191, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (243.35, 176.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728526 reads in 95.644 CPU sec, 3.195 real sec [M::process] read 729026 sequences (100000094 bp) in 3.351 CPU sec, 3.770 real sec... sorting block 67, #reads = 1104229 mem = 536879123 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306779, 2, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (197.56, 65.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 251, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (277.00, 144.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 168, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (201.44, 101.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 728446 sequences (100000063 bp) in 2.777 CPU sec, 2.965 real sec... [M::mem_process_seqs] Processed 729026 reads in 98.769 CPU sec, 3.214 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306673, 6, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 200, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.21, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 253, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (279.38, 145.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 183, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (191.78, 67.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 728006 sequences (100000065 bp) in 2.971 CPU sec, 3.202 real sec... [M::mem_process_seqs] Processed 728446 reads in 93.778 CPU sec, 3.072 real sec sorting block 68, #reads = 1105086 mem = 536876904 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306621, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 250, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (240.47, 105.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (279.21, 144.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 173, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (189.69, 87.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728006 reads in 92.544 CPU sec, 3.017 real sec [M::process] read 727404 sequences (100000001 bp) in 3.704 CPU sec, 4.048 real sec... sorting block 69, #reads = 1103819 mem = 536879477 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306581, 1, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 285, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (225.58, 93.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 256, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (281.00, 145.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 201, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (221.17, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727404 reads in 96.330 CPU sec, 3.144 real sec [M::process] read 727304 sequences (100000070 bp) in 3.304 CPU sec, 3.661 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306724, 3, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 207, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 344) [M::mem_pestat] mean and std.dev: (199.42, 48.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 256, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (280.77, 144.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 189, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 316) [M::mem_pestat] mean and std.dev: (180.50, 41.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 727908 sequences (100000120 bp) in 2.732 CPU sec, 2.908 real sec... [M::mem_process_seqs] Processed 727304 reads in 91.057 CPU sec, 2.962 real sec sorting block 70, #reads = 1104029 mem = 536878158 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 307097, 3, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.64, 58.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (279.10, 144.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 170, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (173.77, 54.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727908 reads in 97.478 CPU sec, 3.170 real sec [M::process] read 727978 sequences (100000120 bp) in 3.633 CPU sec, 4.004 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307032, 1, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 203, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (197.65, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (278.89, 143.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 183, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (194.25, 81.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727978 reads in 94.578 CPU sec, 3.079 real sec sorting block 71, #reads = 1104229 mem = 536881190 peak = 901095424 [M::process] read 727046 sequences (100000104 bp) in 3.797 CPU sec, 4.372 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306628, 2, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 176, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (191.07, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (279.00, 142.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 188, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.47, 65.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727046 reads in 94.390 CPU sec, 3.099 real sec [M::process] read 727432 sequences (100000213 bp) in 2.993 CPU sec, 3.293 real sec... sorting block 72, #reads = 1104584 mem = 536879644 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306977, 1, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 218, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (196.64, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (279.79, 144.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 220, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (281.10, 186.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727432 reads in 94.901 CPU sec, 3.155 real sec [M::process] read 727262 sequences (100000073 bp) in 2.836 CPU sec, 3.327 real sec... sorting block 73, #reads = 1103806 mem = 536879696 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306554, 4, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 225, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (212.26, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (279.19, 143.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 193, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (46, 316) [M::mem_pestat] mean and std.dev: (168.60, 38.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727262 reads in 98.311 CPU sec, 3.220 real sec [M::process] read 727332 sequences (100000161 bp) in 3.265 CPU sec, 3.756 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306665, 2, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 215, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (255.55, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (279.93, 143.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 184, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546) [M::mem_pestat] mean and std.dev: (249.57, 261.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 727332 reads in 95.330 CPU sec, 3.087 real sec [M::process] read 726408 sequences (100000081 bp) in 2.958 CPU sec, 3.302 real sec... sorting block 74, #reads = 1103790 mem = 536879664 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306063, 6, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 187, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (93, 308) [M::mem_pestat] mean and std.dev: (200.11, 38.44) [M::mem_pestat] low and high boundaries for proper pairs: (46, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (283.61, 146.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 231, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 400) [M::mem_pestat] mean and std.dev: (197.09, 47.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 726408 reads in 89.562 CPU sec, 2.917 real sec [M::process] read 725552 sequences (100000284 bp) in 2.911 CPU sec, 3.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305849, 1, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 174, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (187.00, 57.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 263, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (288.04, 148.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 194, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (189.92, 62.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 75, #reads = 1103624 mem = 536880366 peak = 901095424 [M::mem_process_seqs] Processed 725552 reads in 92.307 CPU sec, 3.017 real sec [M::process] read 724950 sequences (100000246 bp) in 3.594 CPU sec, 3.991 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305208, 3, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 156, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (159.07, 73.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (291.40, 150.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 232, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (238.06, 113.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 724950 reads in 93.653 CPU sec, 3.046 real sec [M::process] read 725898 sequences (100000200 bp) in 3.404 CPU sec, 3.679 real sec... sorting block 76, #reads = 1102408 mem = 536879481 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305402, 7, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 201, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (203.82, 94.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (286.02, 147.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 252, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (255.30, 162.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 725898 reads in 92.896 CPU sec, 3.008 real sec [M::process] read 728052 sequences (100000250 bp) in 2.947 CPU sec, 3.252 real sec... sorting block 77, #reads = 1101629 mem = 536880036 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306571, 3, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 166, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (171.72, 62.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (279.80, 146.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 177, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (174.19, 59.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728052 reads in 80.951 CPU sec, 2.643 real sec [M::process] read 729078 sequences (100000027 bp) in 2.891 CPU sec, 3.141 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307140, 4, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 187, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (203.91, 76.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 249, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (276.56, 144.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 182, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (179.89, 63.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 728410 sequences (100000162 bp) in 2.362 CPU sec, 2.533 real sec... [M::mem_process_seqs] Processed 729078 reads in 86.533 CPU sec, 2.795 real sec sorting block 78, #reads = 1103955 mem = 536879483 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307275, 1, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 185, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (203.18, 78.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (277.97, 143.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (212.68, 110.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728410 reads in 92.292 CPU sec, 2.997 real sec [M::process] read 728012 sequences (100000262 bp) in 3.565 CPU sec, 3.996 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306583, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 163, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 314) [M::mem_pestat] mean and std.dev: (155.25, 39.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (278.69, 143.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 204, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (202.06, 64.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728012 reads in 96.285 CPU sec, 3.148 real sec [M::process] read 728468 sequences (100000155 bp) in 3.721 CPU sec, 4.108 real sec... sorting block 79, #reads = 1104923 mem = 536879183 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307080, 6, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 159, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (214.14, 104.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (278.27, 144.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 265, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (219.47, 79.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728468 reads in 94.413 CPU sec, 3.114 real sec [M::process] read 728192 sequences (100000200 bp) in 3.064 CPU sec, 3.363 real sec... sorting block 80, #reads = 1104679 mem = 536879015 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306925, 3, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 194, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (205.18, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (277.51, 143.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 208, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (190.29, 63.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 728192 reads in 90.622 CPU sec, 2.978 real sec [M::process] read 729094 sequences (100000283 bp) in 2.851 CPU sec, 3.049 real sec... sorting block 81, #reads = 1104495 mem = 536876835 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307439, 2, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (214.60, 87.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 251, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (275.07, 141.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 196, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (211.06, 114.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 729210 sequences (100000044 bp) in 2.570 CPU sec, 2.776 real sec... [M::mem_process_seqs] Processed 729094 reads in 85.797 CPU sec, 2.801 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 307485, 4, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1943) [M::mem_pestat] mean and std.dev: (394.95, 247.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (273.82, 141.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 154, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 322) [M::mem_pestat] mean and std.dev: (169.80, 53.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 729210 reads in 88.839 CPU sec, 2.882 real sec [M::process] read 729076 sequences (100000130 bp) in 2.988 CPU sec, 3.188 real sec... sorting block 82, #reads = 1105354 mem = 536879991 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 307630, 5, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 174, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (210.68, 99.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (273.12, 140.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 135, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (155.83, 58.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 729076 reads in 93.537 CPU sec, 3.038 real sec [M::process] read 728964 sequences (100000099 bp) in 2.930 CPU sec, 3.199 real sec... sorting block 83, #reads = 1104993 mem = 536878827 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307421, 6, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (182.81, 71.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (273.29, 140.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 199, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (211.39, 58.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 728962 sequences (100000025 bp) in 2.110 CPU sec, 2.330 real sec... [M::mem_process_seqs] Processed 728964 reads in 77.152 CPU sec, 2.516 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307547, 4, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 167, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.73, 89.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (274.40, 141.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (186.53, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 728658 sequences (100000078 bp) in 2.445 CPU sec, 2.630 real sec... [M::mem_process_seqs] Processed 728962 reads in 84.711 CPU sec, 2.732 real sec sorting block 84, #reads = 1105545 mem = 536880240 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307488, 8, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 168, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 331) [M::mem_pestat] mean and std.dev: (156.57, 43.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (274.89, 141.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 187, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (204.00, 103.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 728424 sequences (100000168 bp) in 2.872 CPU sec, 3.034 real sec... [M::mem_process_seqs] Processed 728658 reads in 89.715 CPU sec, 2.901 real sec sorting block 85, #reads = 1105137 mem = 536880114 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307085, 6, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 166, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (183.64, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 251, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (276.31, 142.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 263, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (265.00, 151.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726618 sequences (100000228 bp) in 2.657 CPU sec, 2.839 real sec... [M::mem_process_seqs] Processed 728424 reads in 87.659 CPU sec, 2.836 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306050, 4, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 200, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 339) [M::mem_pestat] mean and std.dev: (179.28, 40.21) [M::mem_pestat] low and high boundaries for proper pairs: (3, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (283.90, 146.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (115, 224, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (181.70, 64.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 727408 sequences (100000041 bp) in 2.913 CPU sec, 3.077 real sec... [M::mem_process_seqs] Processed 726618 reads in 90.832 CPU sec, 2.944 real sec sorting block 86, #reads = 1105299 mem = 536880080 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306795, 3, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (171.82, 60.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 256, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (281.58, 145.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 235, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.18, 121.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726704 sequences (100000097 bp) in 2.163 CPU sec, 2.334 real sec... [M::mem_process_seqs] Processed 727408 reads in 73.574 CPU sec, 2.390 real sec sorting block 87, #reads = 1103760 mem = 536879373 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306398, 2, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 164, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (205.62, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (282.37, 144.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 161, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (155.18, 46.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 726148 sequences (100000072 bp) in 2.292 CPU sec, 2.429 real sec... [M::mem_process_seqs] Processed 726704 reads in 73.053 CPU sec, 2.369 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 305965, 4, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (153.38, 48.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (285.83, 147.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 206, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (209.69, 77.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 727538 sequences (100000038 bp) in 2.169 CPU sec, 2.326 real sec... [M::mem_process_seqs] Processed 726148 reads in 72.559 CPU sec, 2.354 real sec [M::process] read 40864 sequences (5621610 bp) in 0.365 CPU sec, 0.370 real sec... sorting block 88, #reads = 1103299 mem = 536877859 peak = 901095424 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306237, 4, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 205, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (188.56, 78.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (281.31, 146.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 170, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (191.00, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 17259, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 256, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (281.68, 145.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 727538 reads in 76.024 CPU sec, 2.469 real sec [M::mem_process_seqs] Processed 40864 reads in 12.851 CPU sec, 0.425 real sec sorting block 89, #reads = 1103772 mem = 536878958 peak = 901095424 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 98862984 overall: 69339615232 mem 15376.251 user 1041.918 sys 591.714 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.SH032_HG514.L006\tPU:L006\tSM:SH032_HG514\tLB:SH032_HG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.SH032_HG514_R1.fastq.gz 0003.SH032_HG514_R2.fastq.gz [main] Real time: 591.743 sec; CPU: 16418.172 sec sorting block 90, #reads = 131738 mem = 64038456 peak = 901095424 uniq header count 1 merging 91 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 4406264334 output reads: 99437673 bam_mem_sort: 91 calls 67.929 user 1.837 sys 70.041 real bam_write: 91 calls 39.309 user 3.651 sys 53.036 real execute: 1 calls 22.041 user 1.425 sys 616.325 real merge_files: 1 calls 22.016 user 1.398 sys 24.445 real parse_chunk: 1442 calls 65.608 user 17.771 sys 87.791 real read_chunk: 3905 calls 49.700 user 28.372 sys 480.607 real sort_block: 1 calls 42.637 user 27.792 sys 468.735 real write_chunk: 2461 calls 21.670 user 1.127 sys 23.619 real overall: 2333847552 mem 996.046 user 75.749 sys 616.344 real ls: cannot access '*.cram': No such file or directory