Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ac/7ecc6e2d14d7107e5424353f3ab807/SH032_HG514/0003.SH032_HG514_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d1/e4a03dd9dfd8be820b195b0a30e693/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d1/e4a03dd9dfd8be820b195b0a30e693/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ac/7ecc6e2d14d7107e5424353f3ab807/SH032_HG514/0003.SH032_HG514_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o SH032_HG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1537.480 CPU sec, 57.283 real sec
[M::process] read 728550 sequences (100000081 bp) in 1.174 CPU sec, 1.184 real sec...
[M::process] read 727292 sequences (100000161 bp) in 1.810 CPU sec, 1.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307393, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 188, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (184.53, 52.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (275.20, 141.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 213, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (206.11, 67.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726724 sequences (100000232 bp) in 1.640 CPU sec, 1.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306770, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 159, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432)
[M::mem_pestat] mean and std.dev: (161.89, 53.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 535)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (280.30, 143.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 151, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (161.09, 38.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726110 sequences (100000204 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 728550 reads in 160.446 CPU sec, 5.032 real sec
[M::mem_process_seqs] Processed 727292 reads in 92.763 CPU sec, 2.933 real sec
sorting block 0, #reads = 1105007 mem = 536878022 peak = 595976192
[M::process] read 726094 sequences (100000230 bp) in 1.614 CPU sec, 1.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306483, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 205, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 397)
[M::mem_pestat] mean and std.dev: (229.86, 83.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (280.92, 143.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 217, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (208.06, 78.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726046 sequences (100000171 bp) in 1.600 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306448, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 213, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (256.92, 131.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.27, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 206, 1374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3796)
[M::mem_pestat] mean and std.dev: (496.06, 528.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5007)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306423, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 155, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (176.29, 74.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 503)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.74, 144.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 178, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 359)
[M::mem_pestat] mean and std.dev: (164.67, 49.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726724 reads in 223.274 CPU sec, 7.163 real sec
[M::mem_process_seqs] Processed 726094 reads in 165.313 CPU sec, 5.353 real sec
[M::mem_process_seqs] Processed 726110 reads in 175.112 CPU sec, 5.703 real sec
sorting block 1, #reads = 1103532 mem = 536879225 peak = 765632512
sorting block 2, #reads = 1103113 mem = 536876589 peak = 793473024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305979, 8, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 207, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358)
[M::mem_pestat] mean and std.dev: (267.15, 165.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1757)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.59, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 229, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (58, 378)
[M::mem_pestat] mean and std.dev: (210.83, 49.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 442)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725390 sequences (100000217 bp) in 2.515 CPU sec, 2.582 real sec...
[M::mem_process_seqs] Processed 726046 reads in 85.351 CPU sec, 2.815 real sec
[M::process] read 725764 sequences (100000278 bp) in 2.200 CPU sec, 2.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306014, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 254, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (232.69, 115.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (285.98, 145.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 163, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336)
[M::mem_pestat] mean and std.dev: (159.57, 39.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725714 sequences (100000229 bp) in 1.820 CPU sec, 1.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305802, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 161, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (207.80, 85.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.09, 146.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 205, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (188.42, 76.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725390 reads in 164.760 CPU sec, 5.166 real sec
sorting block 3, #reads = 1102887 mem = 536879683 peak = 793473024
[M::mem_process_seqs] Processed 725764 reads in 93.466 CPU sec, 2.937 real sec
[M::process] read 725278 sequences (100000113 bp) in 1.956 CPU sec, 1.971 real sec...
sorting block 4, #reads = 1102580 mem = 536877234 peak = 924499968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305541, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 198, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 374)
[M::mem_pestat] mean and std.dev: (184.31, 62.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 443)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (285.78, 146.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 326, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (269.67, 101.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724942 sequences (100000194 bp) in 2.425 CPU sec, 2.505 real sec...
[M::process] read 724470 sequences (100000037 bp) in 1.844 CPU sec, 1.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 305752, 8, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 210, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (215.73, 91.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (286.46, 146.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 214, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (189.89, 79.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725714 reads in 166.892 CPU sec, 5.289 real sec
[M::mem_process_seqs] Processed 725278 reads in 99.428 CPU sec, 3.148 real sec
sorting block 5, #reads = 1102801 mem = 536879231 peak = 924499968
[M::process] read 724614 sequences (100000240 bp) in 2.299 CPU sec, 2.344 real sec...
[M::process] read 724290 sequences (100000220 bp) in 1.730 CPU sec, 1.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305591, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 190, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (188.42, 84.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 265, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (288.31, 146.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 177, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (44, 369)
[M::mem_pestat] mean and std.dev: (190.69, 44.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724470 reads in 88.555 CPU sec, 2.813 real sec
sorting block 6, #reads = 1102376 mem = 536877566 peak = 924499968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305043, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 233, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (250.00, 84.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 264, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (287.98, 147.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 220, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (230.13, 108.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724482 sequences (100000083 bp) in 1.363 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305868, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 184, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (191.25, 103.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (289.33, 147.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 210, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (216.22, 64.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724942 reads in 253.643 CPU sec, 8.078 real sec
[M::mem_process_seqs] Processed 724614 reads in 100.606 CPU sec, 3.235 real sec
sorting block 7, #reads = 1101907 mem = 536879737 peak = 924499968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305208, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 242, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (237.67, 64.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (290.27, 147.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 161, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410)
[M::mem_pestat] mean and std.dev: (178.13, 47.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 499)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724034 sequences (100000195 bp) in 2.384 CPU sec, 2.489 real sec...
[M::process] read 723384 sequences (100000031 bp) in 1.650 CPU sec, 1.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304984, 6, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 149, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (170.53, 53.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (291.42, 148.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 195, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (205.55, 75.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 602)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724290 reads in 176.555 CPU sec, 5.629 real sec
[M::mem_process_seqs] Processed 724482 reads in 102.165 CPU sec, 3.254 real sec
sorting block 8, #reads = 1101645 mem = 536879086 peak = 924499968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305003, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 250, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (250.14, 82.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 271, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (293.54, 149.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 220, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (214.22, 89.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725236 sequences (100000164 bp) in 2.414 CPU sec, 2.471 real sec...
[M::process] read 727998 sequences (100000170 bp) in 1.510 CPU sec, 1.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 304845, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 209, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (215.52, 70.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 272, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (294.71, 149.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 173, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (194.64, 64.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 559)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724034 reads in 165.139 CPU sec, 5.256 real sec
sorting block 9, #reads = 1101938 mem = 536878884 peak = 963772416
[M::mem_process_seqs] Processed 723384 reads in 91.503 CPU sec, 2.921 real sec
sorting block 10, #reads = 1100577 mem = 536878852 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305774, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 152, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353)
[M::mem_pestat] mean and std.dev: (164.80, 61.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 427)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (288.90, 147.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 192, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (195.72, 70.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 567)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727016 sequences (100000002 bp) in 2.001 CPU sec, 2.043 real sec...
[M::process] read 726894 sequences (100000160 bp) in 1.450 CPU sec, 1.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306797, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 227, 746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1906)
[M::mem_pestat] mean and std.dev: (343.06, 264.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2486)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.46, 141.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 179, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 338)
[M::mem_pestat] mean and std.dev: (173.44, 37.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725236 reads in 149.817 CPU sec, 4.782 real sec
[M::mem_process_seqs] Processed 727998 reads in 84.791 CPU sec, 2.703 real sec
sorting block 11, #reads = 1101930 mem = 536877757 peak = 963772416
[M::process] read 726632 sequences (100000021 bp) in 2.037 CPU sec, 2.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306446, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 186, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (184.19, 67.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.68, 142.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 191, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 381)
[M::mem_pestat] mean and std.dev: (192.77, 64.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 452)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726320 sequences (100000176 bp) in 1.700 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306663, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 276, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (238.40, 107.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.59, 143.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 168, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (166.68, 55.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306454, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 252, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 397)
[M::mem_pestat] mean and std.dev: (205.44, 58.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.22, 142.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (104, 173, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (172.47, 72.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727016 reads in 248.137 CPU sec, 7.991 real sec
[M::mem_process_seqs] Processed 726632 reads in 180.335 CPU sec, 5.846 real sec
[M::mem_process_seqs] Processed 726894 reads in 188.867 CPU sec, 6.140 real sec
sorting block 12, #reads = 1104350 mem = 536879554 peak = 963772416
sorting block 13, #reads = 1103594 mem = 536879667 peak = 963772416
[M::process] read 725786 sequences (100000138 bp) in 2.278 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306828, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 174, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (180.40, 50.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (283.07, 144.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 172, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (198.54, 90.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725900 sequences (100000163 bp) in 1.986 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306501, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 200, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (187.27, 73.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (284.09, 144.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 187, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (198.62, 88.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726320 reads in 165.211 CPU sec, 5.250 real sec
[M::mem_process_seqs] Processed 725786 reads in 96.887 CPU sec, 3.055 real sec
sorting block 14, #reads = 1103613 mem = 536877736 peak = 963772416
[M::process] read 727234 sequences (100000133 bp) in 2.269 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306516, 1, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 179, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (216.28, 119.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (284.43, 145.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 221, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (206.09, 74.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726408 sequences (100000203 bp) in 2.445 CPU sec, 2.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 306079, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 191, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (199.80, 64.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.63, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 225, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580)
[M::mem_pestat] mean and std.dev: (226.57, 64.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725900 reads in 166.917 CPU sec, 5.284 real sec
[M::process] read 725622 sequences (100000232 bp) in 1.885 CPU sec, 1.896 real sec...
[M::mem_process_seqs] Processed 727234 reads in 95.681 CPU sec, 3.006 real sec
sorting block 15, #reads = 1103318 mem = 536879158 peak = 963772416
sorting block 16, #reads = 1103500 mem = 536879938 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306192, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 181, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (212.92, 107.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.10, 143.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 181, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425)
[M::mem_pestat] mean and std.dev: (152.30, 41.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 522)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725260 sequences (100000169 bp) in 2.277 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305677, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 156, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350)
[M::mem_pestat] mean and std.dev: (160.42, 54.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 724418 sequences (100000144 bp) in 1.930 CPU sec, 1.925 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.24, 145.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 163, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (152.08, 33.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726408 reads in 169.580 CPU sec, 5.390 real sec
[M::mem_process_seqs] Processed 725622 reads in 98.476 CPU sec, 3.122 real sec
sorting block 17, #reads = 1103033 mem = 536876255 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305660, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 263, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (241.80, 95.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (287.87, 147.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 192, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (178.50, 66.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 741)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724046 sequences (100000270 bp) in 2.532 CPU sec, 2.583 real sec...
[M::process] read 724198 sequences (100000105 bp) in 1.800 CPU sec, 1.804 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 305343, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (288.99, 145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 185, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 288)
[M::mem_pestat] mean and std.dev: (176.50, 52.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 386)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725260 reads in 166.381 CPU sec, 5.281 real sec
[M::mem_process_seqs] Processed 724418 reads in 96.048 CPU sec, 3.038 real sec
sorting block 18, #reads = 1102473 mem = 536881413 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 305358, 5, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (290.70, 147.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 153, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 331)
[M::mem_pestat] mean and std.dev: (156.11, 41.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] skip orientation RR
[M::process] read 723722 sequences (100000011 bp) in 2.204 CPU sec, 2.271 real sec...
[M::process] read 723598 sequences (100000234 bp) in 1.760 CPU sec, 1.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305407, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 214, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473)
[M::mem_pestat] mean and std.dev: (195.69, 61.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 582)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (291.10, 147.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 238, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (226.10, 68.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 628)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724046 reads in 164.554 CPU sec, 5.233 real sec
sorting block 19, #reads = 1101928 mem = 536879703 peak = 963772416
[M::mem_process_seqs] Processed 724198 reads in 95.327 CPU sec, 3.027 real sec
sorting block 20, #reads = 1101232 mem = 536880579 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305259, 0, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 181, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450)
[M::mem_pestat] mean and std.dev: (181.14, 63.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 558)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 272, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (293.61, 148.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 218, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 408)
[M::mem_pestat] mean and std.dev: (189.20, 58.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 487)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723244 sequences (100000200 bp) in 2.391 CPU sec, 2.454 real sec...
[M::process] read 726566 sequences (100000089 bp) in 1.750 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 304705, 8, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (293.30, 148.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 149, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (192.06, 85.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723722 reads in 167.981 CPU sec, 5.336 real sec
[M::mem_process_seqs] Processed 723598 reads in 94.872 CPU sec, 2.997 real sec
sorting block 21, #reads = 1101089 mem = 536879017 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 304513, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 181, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (188.56, 60.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (296.25, 149.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 218, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (271.85, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727534 sequences (100000129 bp) in 2.121 CPU sec, 2.166 real sec...
[M::process] read 726710 sequences (100000047 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306479, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 194, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (185.12, 56.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.35, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 186, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (73, 293)
[M::mem_pestat] mean and std.dev: (178.33, 33.50)
[M::mem_pestat] low and high boundaries for proper pairs: (29, 337)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723244 reads in 142.221 CPU sec, 4.517 real sec
[M::mem_process_seqs] Processed 726566 reads in 81.110 CPU sec, 2.567 real sec
sorting block 22, #reads = 1100330 mem = 536880419 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306468, 1, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 154, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355)
[M::mem_pestat] mean and std.dev: (167.54, 64.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.61, 142.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 174, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (184.74, 63.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726488 sequences (100000120 bp) in 2.165 CPU sec, 2.231 real sec...
[M::process] read 726266 sequences (100000172 bp) in 1.519 CPU sec, 1.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306310, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 230, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (206.56, 58.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.61, 143.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 169, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (162.47, 59.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 554)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727534 reads in 142.922 CPU sec, 4.536 real sec
[M::mem_process_seqs] Processed 726710 reads in 81.036 CPU sec, 2.570 real sec
sorting block 23, #reads = 1102542 mem = 536878115 peak = 963772416
sorting block 24, #reads = 1103713 mem = 536877917 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306562, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 152, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410)
[M::mem_pestat] mean and std.dev: (173.36, 58.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.83, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 166, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (174.33, 74.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 482)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726326 sequences (100000056 bp) in 2.319 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306230, 13, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 206, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (199.67, 85.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.60, 145.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (53, 66, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (141.23, 183.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 152, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360)
[M::mem_pestat] mean and std.dev: (154.86, 41.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726488 reads in 141.363 CPU sec, 4.488 real sec
[M::mem_process_seqs] Processed 726266 reads in 82.007 CPU sec, 2.583 real sec
[M::process] read 725716 sequences (100000210 bp) in 1.994 CPU sec, 2.025 real sec...
sorting block 25, #reads = 1103194 mem = 536878910 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306035, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 138, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (148.27, 55.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (284.39, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 179, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (192.39, 61.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726710 sequences (100000006 bp) in 2.031 CPU sec, 2.098 real sec...
[M::process] read 727634 sequences (100000193 bp) in 1.460 CPU sec, 1.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305792, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 181, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566)
[M::mem_pestat] mean and std.dev: (192.65, 77.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (284.95, 145.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 153, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (176.50, 74.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 575)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726326 reads in 148.939 CPU sec, 4.729 real sec
[M::mem_process_seqs] Processed 725716 reads in 85.489 CPU sec, 2.713 real sec
sorting block 26, #reads = 1103365 mem = 536879156 peak = 963772416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306453, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 174, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (205.56, 88.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.55, 144.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 164, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 353)
[M::mem_pestat] mean and std.dev: (165.53, 45.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 422)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726742 sequences (100000083 bp) in 2.012 CPU sec, 2.104 real sec...
[M::process] read 725954 sequences (100000267 bp) in 1.520 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306992, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 229, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (204.80, 69.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.42, 142.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726710 reads in 148.574 CPU sec, 4.731 real sec
[M::mem_process_seqs] Processed 727634 reads in 84.632 CPU sec, 2.684 real sec
sorting block 27, #reads = 1102797 mem = 536878371 peak = 963772416
sorting block 28, #reads = 1104335 mem = 536879450 peak = 980549632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306228, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 177, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (203.76, 88.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (280.22, 143.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 197, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444)
[M::mem_pestat] mean and std.dev: (185.71, 60.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725528 sequences (100000079 bp) in 2.347 CPU sec, 2.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306672, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 238, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (219.21, 69.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (284.65, 145.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 227, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (178.50, 49.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726742 reads in 143.336 CPU sec, 4.554 real sec
[M::mem_process_seqs] Processed 725954 reads in 82.173 CPU sec, 2.585 real sec
[M::process] read 724708 sequences (100000087 bp) in 1.893 CPU sec, 1.901 real sec...
sorting block 29, #reads = 1103529 mem = 536879170 peak = 980549632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306112, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 240, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (232.69, 85.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (285.25, 144.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 203, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (232.21, 120.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723804 sequences (100000012 bp) in 2.160 CPU sec, 2.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305574, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 168, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 342)
[M::mem_pestat] mean and std.dev: (169.13, 44.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 406)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (289.60, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 191, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (241.15, 118.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725528 reads in 137.262 CPU sec, 4.366 real sec
[M::mem_process_seqs] Processed 724708 reads in 78.071 CPU sec, 2.462 real sec
[M::process] read 723628 sequences (100000233 bp) in 2.135 CPU sec, 2.142 real sec...
sorting block 30, #reads = 1102948 mem = 536874605 peak = 999972864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305128, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 144, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 268)
[M::mem_pestat] mean and std.dev: (148.08, 38.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 313)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (291.32, 147.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 184, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 375)
[M::mem_pestat] mean and std.dev: (177.47, 50.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 722898 sequences (100000259 bp) in 2.497 CPU sec, 2.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 305009, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 253, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (238.16, 93.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 272, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (292.91, 147.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 139, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344)
[M::mem_pestat] mean and std.dev: (147.18, 39.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 420)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723804 reads in 142.555 CPU sec, 4.521 real sec
[M::mem_process_seqs] Processed 723628 reads in 84.204 CPU sec, 2.653 real sec
sorting block 31, #reads = 1101868 mem = 536878927 peak = 999972864
[M::process] read 723122 sequences (100000206 bp) in 2.244 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 303925, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 193, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (218.73, 116.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (294.77, 148.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 269, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (223.94, 91.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 32, #reads = 1101160 mem = 536878016 peak = 999972864
[M::process] read 722424 sequences (100000208 bp) in 2.526 CPU sec, 2.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304535, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 201, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453)
[M::mem_pestat] mean and std.dev: (203.00, 99.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (295.08, 148.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 225, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (229.55, 92.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722898 reads in 149.619 CPU sec, 4.754 real sec
[M::mem_process_seqs] Processed 723122 reads in 86.388 CPU sec, 2.715 real sec
[M::process] read 727904 sequences (100000222 bp) in 2.449 CPU sec, 2.484 real sec...
sorting block 33, #reads = 1100770 mem = 536880922 peak = 999972864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304291, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 170, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438)
[M::mem_pestat] mean and std.dev: (176.53, 64.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 277, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (297.96, 149.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 275, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (249.75, 77.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727092 sequences (100000007 bp) in 2.453 CPU sec, 2.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307194, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 180, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (210.70, 97.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.22, 142.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (230.81, 92.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722424 reads in 142.318 CPU sec, 4.514 real sec
[M::mem_process_seqs] Processed 727904 reads in 83.185 CPU sec, 2.624 real sec
sorting block 34, #reads = 1100183 mem = 536880202 peak = 999972864
[M::process] read 726584 sequences (100000004 bp) in 2.504 CPU sec, 2.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306070, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 192, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (204.76, 87.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (280.94, 143.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 179, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (174.00, 51.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726284 sequences (100000160 bp) in 2.487 CPU sec, 2.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306097, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 153, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329)
[M::mem_pestat] mean and std.dev: (165.41, 45.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.35, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (89, 123, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (175.00, 103.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727092 reads in 142.258 CPU sec, 4.529 real sec
[M::mem_process_seqs] Processed 726584 reads in 83.656 CPU sec, 2.645 real sec
sorting block 35, #reads = 1104084 mem = 536879217 peak = 999972864
[M::process] read 726382 sequences (100000058 bp) in 2.754 CPU sec, 2.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306129, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 221, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (230.38, 131.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.45, 144.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 168, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414)
[M::mem_pestat] mean and std.dev: (171.33, 66.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 36, #reads = 1103163 mem = 536880247 peak = 999972864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305994, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 168, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (220.93, 92.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.67, 144.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 196, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 294)
[M::mem_pestat] mean and std.dev: (183.10, 23.76)
[M::mem_pestat] low and high boundaries for proper pairs: (12, 341)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726068 sequences (100000051 bp) in 2.360 CPU sec, 2.373 real sec...
[M::mem_process_seqs] Processed 726284 reads in 144.943 CPU sec, 4.613 real sec
[M::mem_process_seqs] Processed 726382 reads in 84.060 CPU sec, 2.651 real sec
sorting block 37, #reads = 1103298 mem = 536878265 peak = 999972864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306093, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 185, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (194.33, 94.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.38, 144.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 193, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (185.00, 52.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 567)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726068 reads in 77.124 CPU sec, 2.523 real sec
[M::process] read 726828 sequences (100000218 bp) in 2.950 CPU sec, 3.093 real sec...
sorting block 38, #reads = 1102701 mem = 536879930 peak = 999972864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306343, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 182, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (218.05, 118.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (280.28, 143.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 209, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (231.94, 100.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728168 sequences (100000059 bp) in 2.270 CPU sec, 2.463 real sec...
[M::mem_process_seqs] Processed 726828 reads in 78.036 CPU sec, 2.519 real sec
[M::process] read 727878 sequences (100000213 bp) in 2.103 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307194, 9, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 193, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (186.38, 62.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.86, 142.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 199, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (193.22, 56.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727858 sequences (100000187 bp) in 1.480 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306612, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 161, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (195.37, 73.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.11, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 188, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (200.62, 70.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728168 reads in 145.301 CPU sec, 4.572 real sec
[M::mem_process_seqs] Processed 727878 reads in 83.619 CPU sec, 2.633 real sec
sorting block 39, #reads = 1104199 mem = 536878028 peak = 999972864
[M::process] read 727546 sequences (100000250 bp) in 2.048 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307066, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 198, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342)
[M::mem_pestat] mean and std.dev: (173.00, 42.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 411)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.60, 142.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 290, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (266.17, 118.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 40, #reads = 1104583 mem = 536878739 peak = 1003782144
[M::process] read 726796 sequences (100000274 bp) in 2.347 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 307349, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 263, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (237.79, 106.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (279.09, 144.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 156, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (178.91, 47.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 527)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727858 reads in 142.003 CPU sec, 4.543 real sec
[M::mem_process_seqs] Processed 727546 reads in 81.589 CPU sec, 2.574 real sec
[M::process] read 725738 sequences (100000273 bp) in 1.972 CPU sec, 2.081 real sec...
sorting block 41, #reads = 1104398 mem = 536879183 peak = 1003782144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306652, 1, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (214.74, 93.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (280.92, 144.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 185, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (206.00, 74.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724172 sequences (100000208 bp) in 1.843 CPU sec, 1.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306161, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (218.31, 101.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.58, 145.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 203, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (184.40, 74.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726796 reads in 139.447 CPU sec, 4.424 real sec
[M::process] read 723448 sequences (100000151 bp) in 1.526 CPU sec, 1.530 real sec...
[M::mem_process_seqs] Processed 725738 reads in 79.947 CPU sec, 2.515 real sec
sorting block 42, #reads = 1104174 mem = 536880746 peak = 1003782144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304967, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 256, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (255.92, 113.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (289.24, 145.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (103, 146, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (178.19, 94.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 671)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 722940 sequences (100000162 bp) in 2.044 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304272, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 232, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (223.35, 68.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (292.63, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 254, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (235.80, 81.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 722448 sequences (100000176 bp) in 1.560 CPU sec, 1.557 real sec...
[M::mem_process_seqs] Processed 724172 reads in 139.197 CPU sec, 4.423 real sec
[M::mem_process_seqs] Processed 723448 reads in 80.016 CPU sec, 2.527 real sec
sorting block 43, #reads = 1102609 mem = 536879603 peak = 1003782144
sorting block 44, #reads = 1100890 mem = 536879494 peak = 1003782144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304581, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 191, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (188.88, 65.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (294.50, 148.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 221, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (226.43, 79.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727756 sequences (100000119 bp) in 1.918 CPU sec, 1.958 real sec...
[M::process] read 728334 sequences (100000062 bp) in 1.440 CPU sec, 1.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304025, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 218, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (239.15, 108.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (297.26, 149.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 195, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (177.36, 56.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 599)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722940 reads in 133.953 CPU sec, 4.257 real sec
[M::mem_process_seqs] Processed 722448 reads in 76.623 CPU sec, 2.423 real sec
sorting block 45, #reads = 1100429 mem = 536878064 peak = 1003782144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306730, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 259, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (136, 386)
[M::mem_pestat] mean and std.dev: (243.92, 52.49)
[M::mem_pestat] low and high boundaries for proper pairs: (34, 454)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (281.10, 145.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 238, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (213.62, 61.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 623)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728014 sequences (100000085 bp) in 2.502 CPU sec, 2.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 306495, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 221, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (231.26, 84.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (278.88, 144.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 169, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (182.00, 64.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 573)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727756 reads in 135.745 CPU sec, 4.299 real sec
[M::process] read 727390 sequences (100000199 bp) in 1.595 CPU sec, 1.593 real sec...
[M::mem_process_seqs] Processed 728334 reads in 78.711 CPU sec, 2.474 real sec
sorting block 46, #reads = 1102853 mem = 536879076 peak = 1003782144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306774, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 175, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (197.00, 94.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (280.08, 145.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 261, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (232.77, 81.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 647)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727074 sequences (100000224 bp) in 2.240 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306487, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 192, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (178.81, 48.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.50, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 198, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (261.59, 151.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728014 reads in 135.055 CPU sec, 4.285 real sec
[M::process] read 726358 sequences (100000123 bp) in 1.450 CPU sec, 1.453 real sec...
[M::mem_process_seqs] Processed 727390 reads in 77.096 CPU sec, 2.429 real sec
sorting block 47, #reads = 1104727 mem = 536876530 peak = 1130156032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306205, 0, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 199, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (216.53, 100.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (282.74, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 203, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (226.67, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726222 sequences (100000139 bp) in 2.708 CPU sec, 2.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305972, 0, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 223, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (183.50, 80.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.49, 146.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 184, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (222.35, 121.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727074 reads in 133.020 CPU sec, 4.216 real sec
sorting block 48, #reads = 1104528 mem = 536878196 peak = 1135955968
[M::mem_process_seqs] Processed 726358 reads in 77.309 CPU sec, 2.432 real sec
[M::process] read 726190 sequences (100000015 bp) in 2.284 CPU sec, 2.323 real sec...
sorting block 49, #reads = 1103447 mem = 536879877 peak = 1135955968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306177, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (214.75, 69.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.57, 146.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 202, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (231.81, 129.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1353)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726342 sequences (100000062 bp) in 2.205 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305790, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 203, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (216.00, 94.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.85, 145.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 154, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371)
[M::mem_pestat] mean and std.dev: (170.76, 64.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726222 reads in 138.287 CPU sec, 4.394 real sec
[M::mem_process_seqs] Processed 726190 reads in 80.279 CPU sec, 2.525 real sec
[M::process] read 725778 sequences (100000229 bp) in 2.393 CPU sec, 2.416 real sec...
sorting block 50, #reads = 1103123 mem = 536879508 peak = 1135955968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306043, 3, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 245, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (228.00, 77.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.05, 145.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 185, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (181.24, 61.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725320 sequences (100000094 bp) in 2.667 CPU sec, 2.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305478, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 164, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (179.72, 63.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 658)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.17, 145.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (226.42, 77.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726342 reads in 156.356 CPU sec, 4.969 real sec
[M::mem_process_seqs] Processed 725778 reads in 90.972 CPU sec, 2.860 real sec
sorting block 51, #reads = 1103259 mem = 536878493 peak = 1135955968
[M::process] read 725020 sequences (100000284 bp) in 2.477 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305686, 6, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 172, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (191.47, 72.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (287.04, 146.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 177, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (189.04, 72.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725034 sequences (100000095 bp) in 2.484 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305052, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 227, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (192.45, 69.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (289.79, 148.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 238, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (239.20, 131.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725320 reads in 153.874 CPU sec, 4.885 real sec
sorting block 52, #reads = 1102593 mem = 536878253 peak = 1135955968
[M::mem_process_seqs] Processed 725020 reads in 88.674 CPU sec, 2.788 real sec
[M::process] read 725338 sequences (100000101 bp) in 2.202 CPU sec, 2.235 real sec...
sorting block 53, #reads = 1102171 mem = 536876992 peak = 1135955968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305589, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 173, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (180.53, 78.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (289.11, 148.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 194, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (196.11, 68.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725278 sequences (100000139 bp) in 2.522 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305367, 8, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 203, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (201.44, 82.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (288.87, 148.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 246, 1388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3804)
[M::mem_pestat] mean and std.dev: (785.76, 1015.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5012)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725034 reads in 155.163 CPU sec, 4.927 real sec
[M::mem_process_seqs] Processed 725338 reads in 90.432 CPU sec, 2.844 real sec
[M::process] read 725084 sequences (100000178 bp) in 2.385 CPU sec, 2.432 real sec...
sorting block 54, #reads = 1102121 mem = 536880672 peak = 1135955968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305121, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 139, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (197.47, 90.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (290.94, 150.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 204, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (230.00, 74.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725310 sequences (100000247 bp) in 2.455 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305461, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 203, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 414)
[M::mem_pestat] mean and std.dev: (194.80, 57.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (292.34, 152.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 156, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393)
[M::mem_pestat] mean and std.dev: (180.60, 52.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725278 reads in 153.544 CPU sec, 4.888 real sec
[M::mem_process_seqs] Processed 725084 reads in 90.574 CPU sec, 2.856 real sec
sorting block 55, #reads = 1102127 mem = 536876272 peak = 1141821440
[M::process] read 728754 sequences (100000043 bp) in 2.043 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305415, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 173, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (193.27, 83.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (292.80, 154.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 249, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (240.06, 93.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728580 sequences (100000038 bp) in 2.766 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307183, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 190, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355)
[M::mem_pestat] mean and std.dev: (167.06, 36.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 431)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 252, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (280.39, 147.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 257, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (210.55, 70.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725310 reads in 151.460 CPU sec, 4.807 real sec
sorting block 56, #reads = 1102193 mem = 536879434 peak = 1141821440
[M::mem_process_seqs] Processed 728754 reads in 86.564 CPU sec, 2.722 real sec
[M::process] read 727958 sequences (100000237 bp) in 1.887 CPU sec, 1.916 real sec...
sorting block 57, #reads = 1103159 mem = 536880780 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307326, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 192, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531)
[M::mem_pestat] mean and std.dev: (184.00, 67.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (278.42, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 203, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (217.10, 82.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727506 sequences (100000111 bp) in 2.514 CPU sec, 2.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306707, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 195, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 360)
[M::mem_pestat] mean and std.dev: (192.69, 57.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.30, 144.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 193, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410)
[M::mem_pestat] mean and std.dev: (197.47, 65.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728580 reads in 147.940 CPU sec, 4.705 real sec
[M::mem_process_seqs] Processed 727958 reads in 84.443 CPU sec, 2.660 real sec
[M::process] read 726990 sequences (100000007 bp) in 2.469 CPU sec, 2.505 real sec...
sorting block 58, #reads = 1105348 mem = 536880273 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306451, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 151, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (199.91, 80.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 257, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (281.41, 145.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 219, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (200.82, 66.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726446 sequences (100000177 bp) in 2.407 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306795, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 209, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531)
[M::mem_pestat] mean and std.dev: (200.94, 74.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.37, 145.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 246, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (244.88, 93.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727506 reads in 148.347 CPU sec, 4.714 real sec
[M::mem_process_seqs] Processed 726990 reads in 86.625 CPU sec, 2.726 real sec
[M::process] read 726220 sequences (100000041 bp) in 1.970 CPU sec, 1.999 real sec...
sorting block 59, #reads = 1104254 mem = 536877677 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306484, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 254, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (216.46, 81.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.10, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 173, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (177.79, 71.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726576 sequences (100000137 bp) in 2.331 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305728, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (93, 156, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414)
[M::mem_pestat] mean and std.dev: (142.50, 44.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 521)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.02, 145.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 193, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (191.61, 64.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726446 reads in 148.897 CPU sec, 4.736 real sec
[M::mem_process_seqs] Processed 726220 reads in 85.296 CPU sec, 2.685 real sec
sorting block 60, #reads = 1103839 mem = 536878303 peak = 1144750080
[M::process] read 727626 sequences (100000038 bp) in 2.566 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307013, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 173, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (174.62, 57.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.41, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 160, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 339)
[M::mem_pestat] mean and std.dev: (187.15, 54.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 406)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 61, #reads = 1103345 mem = 536876794 peak = 1144750080
[M::process] read 726560 sequences (100000075 bp) in 2.475 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306457, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 219, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (203.79, 91.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.59, 143.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 218, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (204.65, 67.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726576 reads in 149.928 CPU sec, 4.766 real sec
[M::mem_process_seqs] Processed 727626 reads in 86.770 CPU sec, 2.731 real sec
[M::process] read 726172 sequences (100000167 bp) in 2.497 CPU sec, 2.608 real sec...
sorting block 62, #reads = 1103388 mem = 536878488 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306073, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 158, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (176.11, 76.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.39, 145.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 253, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (273.50, 136.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725696 sequences (100000034 bp) in 2.631 CPU sec, 2.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306041, 8, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614)
[M::mem_pestat] mean and std.dev: (353.86, 283.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2096)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (285.68, 146.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 195, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (196.29, 81.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 629)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726560 reads in 149.322 CPU sec, 4.745 real sec
[M::mem_process_seqs] Processed 726172 reads in 86.587 CPU sec, 2.725 real sec
sorting block 63, #reads = 1104342 mem = 536878537 peak = 1144750080
[M::process] read 725344 sequences (100000056 bp) in 2.718 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305419, 11, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 241, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (288.67, 131.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (287.95, 148.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (56, 342, 9722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 29054)
[M::mem_pestat] mean and std.dev: (2797.36, 4245.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38720)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 172, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (186.75, 65.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 725436 sequences (100000261 bp) in 2.538 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305365, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 172, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349)
[M::mem_pestat] mean and std.dev: (166.08, 47.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (289.55, 149.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 208, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (197.15, 80.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725696 reads in 145.002 CPU sec, 4.607 real sec
[M::mem_process_seqs] Processed 725344 reads in 82.154 CPU sec, 2.591 real sec
sorting block 64, #reads = 1102918 mem = 536878037 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305208, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 132, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (163.14, 75.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (289.16, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 188, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 345)
[M::mem_pestat] mean and std.dev: (180.81, 44.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 403)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725090 sequences (100000019 bp) in 2.966 CPU sec, 3.084 real sec...
sorting block 65, #reads = 1101907 mem = 536877880 peak = 1144750080
[M::mem_process_seqs] Processed 725436 reads in 79.405 CPU sec, 2.602 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305545, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 208, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (210.42, 90.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (291.55, 150.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 188, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (179.25, 54.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 533)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725090 reads in 74.283 CPU sec, 2.420 real sec
sorting block 66, #reads = 1101509 mem = 536878046 peak = 1144750080
[M::process] read 724988 sequences (100000078 bp) in 2.837 CPU sec, 2.995 real sec...
[M::process] read 724994 sequences (100000277 bp) in 2.057 CPU sec, 2.101 real sec...
[M::process] read 727870 sequences (100000063 bp) in 1.568 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305233, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 186, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (237.14, 129.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (293.45, 153.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 194, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (185.09, 56.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724994 reads in 75.968 CPU sec, 2.392 real sec
sorting block 67, #reads = 1102267 mem = 536878210 peak = 1144750080
[M::process] read 728352 sequences (100000161 bp) in 2.439 CPU sec, 2.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 304889, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 193, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 342)
[M::mem_pestat] mean and std.dev: (169.71, 44.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 406)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (292.66, 152.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 259, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (261.00, 87.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 307446, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 178, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (180.56, 66.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (283.91, 150.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 203, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413)
[M::mem_pestat] mean and std.dev: (183.80, 47.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 501)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724988 reads in 224.346 CPU sec, 7.072 real sec
[M::mem_process_seqs] Processed 727870 reads in 96.152 CPU sec, 3.049 real sec
[M::process] read 727428 sequences (100000043 bp) in 2.541 CPU sec, 2.570 real sec...
sorting block 68, #reads = 1102102 mem = 536879601 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306491, 0, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 193, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (204.00, 85.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (279.12, 145.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 232, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 374)
[M::mem_pestat] mean and std.dev: (216.82, 55.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 440)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726874 sequences (100000084 bp) in 2.372 CPU sec, 2.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306120, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (105, 166, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (202.89, 106.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (281.03, 144.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 205, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (216.00, 83.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728352 reads in 147.908 CPU sec, 4.709 real sec
[M::mem_process_seqs] Processed 727428 reads in 84.974 CPU sec, 2.680 real sec
sorting block 69, #reads = 1104603 mem = 536879708 peak = 1144750080
[M::process] read 726844 sequences (100000200 bp) in 1.953 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306175, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 201, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 375)
[M::mem_pestat] mean and std.dev: (208.29, 55.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 445)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (282.22, 145.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 192, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408)
[M::mem_pestat] mean and std.dev: (195.62, 74.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726702 sequences (100000122 bp) in 2.313 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306524, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 221, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (212.63, 88.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (283.19, 146.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 205, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (201.27, 44.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726874 reads in 146.663 CPU sec, 4.653 real sec
[M::mem_process_seqs] Processed 726844 reads in 84.248 CPU sec, 2.659 real sec
sorting block 70, #reads = 1103336 mem = 536880514 peak = 1144750080
[M::process] read 726538 sequences (100000041 bp) in 1.795 CPU sec, 1.809 real sec...
sorting block 71, #reads = 1103793 mem = 536877580 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306231, 9, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 318, 4221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12335)
[M::mem_pestat] mean and std.dev: (1414.71, 1888.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16392)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.90, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 198, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 315)
[M::mem_pestat] mean and std.dev: (176.82, 46.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 366)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727652 sequences (100000245 bp) in 2.041 CPU sec, 2.120 real sec...
[M::process] read 728692 sequences (100000228 bp) in 1.500 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306149, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 216, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 392)
[M::mem_pestat] mean and std.dev: (217.95, 61.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.37, 146.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 200, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 371)
[M::mem_pestat] mean and std.dev: (198.53, 55.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 440)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726702 reads in 146.453 CPU sec, 4.655 real sec
[M::mem_process_seqs] Processed 726538 reads in 84.287 CPU sec, 2.668 real sec
sorting block 72, #reads = 1103755 mem = 536878592 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306777, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 156, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407)
[M::mem_pestat] mean and std.dev: (159.65, 57.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (279.70, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 194, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (212.70, 94.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727702 sequences (100000252 bp) in 2.941 CPU sec, 3.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307174, 1, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 222, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (176.06, 60.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 546)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (275.42, 142.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 178, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356)
[M::mem_pestat] mean and std.dev: (164.80, 49.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 431)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727652 reads in 145.211 CPU sec, 4.600 real sec
[M::mem_process_seqs] Processed 728692 reads in 83.659 CPU sec, 2.633 real sec
sorting block 73, #reads = 1103600 mem = 536879765 peak = 1144750080
[M::process] read 727054 sequences (100000263 bp) in 2.546 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306825, 2, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 160, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (265.38, 185.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.72, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 206, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (247.80, 123.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725994 sequences (100000045 bp) in 2.532 CPU sec, 2.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306388, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 168, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 328)
[M::mem_pestat] mean and std.dev: (171.00, 46.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 388)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.24, 146.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 193, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (211.74, 85.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727702 reads in 146.951 CPU sec, 4.672 real sec
[M::mem_process_seqs] Processed 727054 reads in 86.183 CPU sec, 2.713 real sec
sorting block 74, #reads = 1105034 mem = 536878011 peak = 1144750080
[M::process] read 725646 sequences (100000114 bp) in 2.309 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306034, 4, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 231, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (226.80, 73.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (286.19, 147.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 232, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (214.52, 77.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 75, #reads = 1102757 mem = 536880419 peak = 1144750080
[M::process] read 724760 sequences (100000166 bp) in 2.223 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305419, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 169, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (167.86, 56.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (287.82, 148.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 725994 reads in 149.016 CPU sec, 4.736 real sec
[M::mem_process_seqs] Processed 725646 reads in 87.265 CPU sec, 2.750 real sec
[M::process] read 724718 sequences (100000146 bp) in 1.697 CPU sec, 1.719 real sec...
sorting block 76, #reads = 1103271 mem = 536880836 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305243, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 217, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (205.69, 73.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (291.27, 149.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 190, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (200.27, 100.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724740 sequences (100000043 bp) in 2.366 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305690, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 1441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3845)
[M::mem_pestat] mean and std.dev: (398.71, 339.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5047)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (292.96, 151.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (247.83, 110.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 729)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724760 reads in 147.601 CPU sec, 4.696 real sec
[M::process] read 725126 sequences (100000210 bp) in 1.602 CPU sec, 1.614 real sec...
sorting block 77, #reads = 1101860 mem = 536878274 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 305172, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 176, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (189.11, 76.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (293.17, 152.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 237, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (206.73, 71.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724740 reads in 76.039 CPU sec, 2.405 real sec
[M::process] read 727508 sequences (100000071 bp) in 2.662 CPU sec, 2.689 real sec...
[M::mem_process_seqs] Processed 724718 reads in 201.438 CPU sec, 6.363 real sec
sorting block 78, #reads = 1101995 mem = 536878510 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305410, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 192, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (200.33, 63.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 553)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (293.27, 153.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 202, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (164.54, 47.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727716 sequences (100000067 bp) in 2.348 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305904, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 175, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 349)
[M::mem_pestat] mean and std.dev: (183.80, 49.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 256, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (283.77, 149.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 211, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 358)
[M::mem_pestat] mean and std.dev: (207.75, 33.17)
[M::mem_pestat] low and high boundaries for proper pairs: (22, 414)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725126 reads in 149.569 CPU sec, 4.724 real sec
[M::mem_process_seqs] Processed 727508 reads in 83.393 CPU sec, 2.630 real sec
sorting block 79, #reads = 1101435 mem = 536879840 peak = 1144750080
[M::process] read 727372 sequences (100000149 bp) in 2.039 CPU sec, 2.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306681, 9, 21)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (281.02, 145.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 183, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (176.26, 66.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 551)
[M::mem_pestat] skip orientation RR
[M::process] read 726708 sequences (100000207 bp) in 1.967 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306502, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 167, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 362)
[M::mem_pestat] mean and std.dev: (183.09, 50.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 430)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.13, 145.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 145, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (134.82, 44.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 498)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727716 reads in 145.888 CPU sec, 4.624 real sec
[M::process] read 726776 sequences (100000133 bp) in 1.538 CPU sec, 1.544 real sec...
[M::mem_process_seqs] Processed 727372 reads in 83.984 CPU sec, 2.645 real sec
sorting block 80, #reads = 1103114 mem = 536882220 peak = 1144750080
sorting block 81, #reads = 1104065 mem = 536876999 peak = 1144750080
[M::process] read 726518 sequences (100000166 bp) in 2.510 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305847, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 168, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (183.56, 62.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.20, 145.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 216, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (201.82, 66.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726776 reads in 76.158 CPU sec, 2.399 real sec
[M::process] read 726838 sequences (100000116 bp) in 1.809 CPU sec, 1.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305865, 5, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 198, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (182.53, 45.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.37, 145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 166, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (242.60, 155.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306399, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 181, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453)
[M::mem_pestat] mean and std.dev: (197.64, 68.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 550)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.82, 144.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 199, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (212.83, 100.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726708 reads in 216.904 CPU sec, 6.892 real sec
[M::process] read 728580 sequences (100000233 bp) in 1.674 CPU sec, 1.684 real sec...
[M::mem_process_seqs] Processed 726518 reads in 88.541 CPU sec, 2.809 real sec
sorting block 82, #reads = 1103200 mem = 536878918 peak = 1144750080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306477, 4, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 217, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (204.63, 86.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.64, 143.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 166, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (168.90, 61.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 498)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 83, #reads = 1103774 mem = 536877321 peak = 1148727296
[M::process] read 728340 sequences (100000127 bp) in 2.702 CPU sec, 2.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306875, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 203, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (215.53, 79.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (275.77, 142.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 204, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (207.91, 80.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726838 reads in 140.202 CPU sec, 4.466 real sec
[M::mem_process_seqs] Processed 728580 reads in 80.605 CPU sec, 2.556 real sec
[M::process] read 728514 sequences (100000145 bp) in 2.040 CPU sec, 2.147 real sec...
sorting block 84, #reads = 1103639 mem = 536880241 peak = 1148727296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 307112, 1, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 163, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (210.82, 116.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (276.09, 142.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 206, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 379)
[M::mem_pestat] mean and std.dev: (186.19, 60.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 452)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728150 sequences (100000131 bp) in 2.339 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307199, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 197, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (181.47, 71.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (275.09, 141.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 157, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (189.55, 74.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728340 reads in 138.570 CPU sec, 4.397 real sec
[M::mem_process_seqs] Processed 728514 reads in 81.420 CPU sec, 2.568 real sec
[M::process] read 727230 sequences (100000197 bp) in 1.935 CPU sec, 1.954 real sec...
sorting block 85, #reads = 1104726 mem = 536878667 peak = 1148727296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306913, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 196, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (212.06, 104.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.62, 142.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 206, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 352)
[M::mem_pestat] mean and std.dev: (211.22, 24.15)
[M::mem_pestat] low and high boundaries for proper pairs: (46, 403)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726526 sequences (100000189 bp) in 2.158 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306100, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 193, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (197.15, 95.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (280.40, 144.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 208, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (200.06, 80.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728150 reads in 140.744 CPU sec, 4.482 real sec
[M::mem_process_seqs] Processed 727230 reads in 81.685 CPU sec, 2.579 real sec
[M::process] read 725104 sequences (100000195 bp) in 1.708 CPU sec, 1.730 real sec...
sorting block 86, #reads = 1103545 mem = 536878022 peak = 1148727296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305847, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 192, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (197.08, 72.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.83, 145.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 201, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (185.00, 79.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 87, #reads = 1103046 mem = 536875533 peak = 1148727296
[M::process] read 724602 sequences (100000105 bp) in 2.123 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304954, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 287, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073)
[M::mem_pestat] mean and std.dev: (270.78, 125.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1370)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (288.64, 147.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 181, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (190.69, 65.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726526 reads in 141.785 CPU sec, 4.503 real sec
[M::mem_process_seqs] Processed 725104 reads in 81.761 CPU sec, 2.576 real sec
[M::process] read 724092 sequences (100000218 bp) in 2.159 CPU sec, 2.188 real sec...
sorting block 88, #reads = 1102331 mem = 536880741 peak = 1148727296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304955, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 252, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (247.35, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (292.58, 150.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 724450 sequences (100000025 bp) in 2.118 CPU sec, 2.199 real sec...
[M::process] read 261160 sequences (36029560 bp) in 0.720 CPU sec, 0.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 304843, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 224, 647)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613)
[M::mem_pestat] mean and std.dev: (272.44, 185.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2096)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 269, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (294.11, 151.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 185, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431)
[M::mem_pestat] mean and std.dev: (182.75, 61.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 525)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724602 reads in 139.799 CPU sec, 4.465 real sec
[M::mem_process_seqs] Processed 724092 reads in 79.500 CPU sec, 2.528 real sec
sorting block 89, #reads = 1101773 mem = 536879843 peak = 1148727296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304721, 3, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 153, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (179.06, 68.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (294.35, 152.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 189, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (223.39, 108.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 109831, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 175, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347)
[M::mem_pestat] mean and std.dev: (170.33, 38.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 417)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (295.10, 154.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 724450 reads in 93.230 CPU sec, 3.035 real sec
[M::mem_process_seqs] Processed 261160 reads in 32.005 CPU sec, 1.040 real sec
sorting block 90, #reads = 1101479 mem = 536882036 peak = 1224728576
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 100461256
overall: 69805113344 mem 12079.999 user 361.855 sys 413.214 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.SH032_HG514.L002\tPU:L002\tSM:SH032_HG514\tLB:SH032_HG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.SH032_HG514_R1.fastq.gz 0003.SH032_HG514_R2.fastq.gz
[main] Real time: 413.253 sec; CPU: 12441.858 sec
sorting block 91, #reads = 679935 mem = 331402490 peak = 1224728576
uniq header count 1
merging 92 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 4441552538
output reads: 101045271
bam_mem_sort: 92 calls 45.827 user 0.529 sys 46.483 real
bam_write: 92 calls 29.685 user 1.486 sys 31.386 real
execute: 1 calls 24.385 user 1.123 sys 442.608 real
merge_files: 1 calls 24.367 user 1.079 sys 28.712 real
parse_chunk: 1466 calls 77.312 user 17.386 sys 95.891 real
read_chunk: 3969 calls 36.828 user 26.601 sys 364.394 real
sort_block: 1 calls 27.964 user 26.215 sys 350.366 real
write_chunk: 2501 calls 23.975 user 0.883 sys 27.996 real
overall: 2659844096 mem 1149.930 user 58.699 sys 442.630 real
ls: cannot access '*.cram': No such file or directory