.command.err - Workdir - DAQ:SENTIEON_BWAMEM (SH032_HG514;L008;0004)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fb/b00e9a68157d19be91d677b1ba3bac/.command.err
Size: 195.4 KB
Task: DAQ:SENTIEON_BWAMEM (SH032_HG514;L008;0004)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o SH032_HG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2395.390 CPU sec, 83.363 real sec
[M::process] read 727644 sequences (100000046 bp) in 1.405 CPU sec, 1.412 real sec...
[M::process] read 726706 sequences (100000287 bp) in 1.781 CPU sec, 1.810 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307210, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 160, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (174.79, 61.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (278.79, 143.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 230, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (212.13, 65.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726224 sequences (100000072 bp) in 1.629 CPU sec, 1.674 real sec...
[M::process] read 726028 sequences (100000005 bp) in 1.578 CPU sec, 1.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306219, 9, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 164, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (173.85, 66.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.66, 144.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (194.62, 73.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 655)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727644 reads in 190.075 CPU sec, 6.108 real sec
[M::mem_process_seqs] Processed 726706 reads in 93.574 CPU sec, 3.008 real sec
sorting block 0, #reads = 1104002 mem = 536879209 peak = 581980160
[M::process] read 725760 sequences (100000051 bp) in 1.803 CPU sec, 1.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 306871, 7, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 211, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (242.47, 116.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.14, 144.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 195, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (186.32, 71.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725116 sequences (100000279 bp) in 1.510 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306543, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 271, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (266.62, 72.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (283.66, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 192, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (213.50, 96.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306346, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 201, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (214.38, 67.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.14, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 151, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336)
[M::mem_pestat] mean and std.dev: (153.82, 41.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726224 reads in 230.447 CPU sec, 7.401 real sec
[M::mem_process_seqs] Processed 726028 reads in 163.142 CPU sec, 5.266 real sec
[M::mem_process_seqs] Processed 725760 reads in 170.759 CPU sec, 5.544 real sec
sorting block 1, #reads = 1103361 mem = 536878544 peak = 739266560
sorting block 2, #reads = 1103373 mem = 536878710 peak = 885096448
[M::process] read 724874 sequences (100000104 bp) in 2.371 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 306031, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 208, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (211.28, 67.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (285.45, 144.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 151, 156)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (81, 206)
[M::mem_pestat] mean and std.dev: (142.89, 23.97)
[M::mem_pestat] low and high boundaries for proper pairs: (47, 239)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724230 sequences (100000259 bp) in 1.774 CPU sec, 1.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 305544, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 187, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (198.36, 86.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (286.63, 145.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 244, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (206.50, 62.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 683)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725116 reads in 162.930 CPU sec, 5.202 real sec
[M::mem_process_seqs] Processed 724874 reads in 92.331 CPU sec, 2.923 real sec
[M::process] read 723792 sequences (100000187 bp) in 1.755 CPU sec, 1.798 real sec...
sorting block 3, #reads = 1102416 mem = 536878996 peak = 885096448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305238, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 170, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (174.57, 59.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (287.56, 144.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 164, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (200.28, 98.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724174 sequences (100000116 bp) in 2.173 CPU sec, 2.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 304811, 1, 21)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (290.09, 145.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 165, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 280)
[M::mem_pestat] mean and std.dev: (160.90, 37.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 327)
[M::mem_pestat] skip orientation RR
[M::process] read 723568 sequences (100000065 bp) in 1.646 CPU sec, 1.648 real sec...
[M::mem_process_seqs] Processed 724230 reads in 158.888 CPU sec, 5.052 real sec
[M::mem_process_seqs] Processed 723792 reads in 91.446 CPU sec, 2.889 real sec
sorting block 4, #reads = 1101659 mem = 536879127 peak = 885096448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 305244, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 218, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (227.25, 144.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (289.93, 146.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 255, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (243.41, 85.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723578 sequences (100000158 bp) in 1.935 CPU sec, 2.025 real sec...
[M::process] read 724094 sequences (100000238 bp) in 1.536 CPU sec, 1.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305316, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 209, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (225.46, 88.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (291.74, 146.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (283.19, 140.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724174 reads in 160.490 CPU sec, 5.145 real sec
sorting block 5, #reads = 1100825 mem = 536879893 peak = 885096448
[M::mem_process_seqs] Processed 723568 reads in 90.845 CPU sec, 2.885 real sec
sorting block 6, #reads = 1100766 mem = 536878399 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 304976, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 200, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434)
[M::mem_pestat] mean and std.dev: (171.64, 50.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 533)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 269, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (290.27, 145.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 278, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (248.14, 128.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723228 sequences (100000031 bp) in 2.067 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304825, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 142, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (172.57, 73.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (289.54, 145.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 183, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (201.22, 62.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 563)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723004 sequences (100000017 bp) in 1.642 CPU sec, 1.647 real sec...
[M::mem_process_seqs] Processed 723578 reads in 158.478 CPU sec, 5.074 real sec
[M::mem_process_seqs] Processed 724094 reads in 90.794 CPU sec, 2.870 real sec
sorting block 7, #reads = 1101086 mem = 536877717 peak = 896155648
[M::process] read 723082 sequences (100000074 bp) in 2.093 CPU sec, 2.211 real sec...
[M::process] read 722860 sequences (100000178 bp) in 1.574 CPU sec, 1.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304509, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 256, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (223.22, 97.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 274, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (296.76, 150.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 235, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (223.35, 103.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723004 reads in 83.180 CPU sec, 2.627 real sec
sorting block 8, #reads = 1100770 mem = 536878633 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305041, 6, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 297, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (257.65, 109.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (292.93, 147.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 182, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (190.26, 85.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 304569, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 227, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (263.92, 129.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (296.63, 150.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 252, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (224.05, 110.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723398 sequences (100000066 bp) in 1.629 CPU sec, 1.634 real sec...
[M::mem_process_seqs] Processed 723228 reads in 239.774 CPU sec, 7.674 real sec
[M::mem_process_seqs] Processed 723082 reads in 98.549 CPU sec, 3.196 real sec
sorting block 9, #reads = 1100513 mem = 536878763 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304558, 9, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 177, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (198.31, 89.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 275, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (297.55, 151.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 185, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (206.29, 78.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727618 sequences (100000169 bp) in 2.451 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304834, 1, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 213, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438)
[M::mem_pestat] mean and std.dev: (187.83, 48.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 272, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (294.60, 149.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 182, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (226.76, 127.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722860 reads in 165.251 CPU sec, 5.324 real sec
[M::mem_process_seqs] Processed 723398 reads in 96.958 CPU sec, 3.081 real sec
[M::process] read 727014 sequences (100000190 bp) in 1.856 CPU sec, 1.919 real sec...
sorting block 10, #reads = 1100654 mem = 536879724 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306951, 6, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 186, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (197.08, 73.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (279.90, 144.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 204, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (230.17, 94.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726398 sequences (100000120 bp) in 2.331 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306395, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 185, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (171.00, 90.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.47, 144.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 255, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (231.25, 73.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726764 sequences (100000087 bp) in 1.586 CPU sec, 1.587 real sec...
[M::mem_process_seqs] Processed 727618 reads in 168.949 CPU sec, 5.460 real sec
sorting block 11, #reads = 1101045 mem = 536878899 peak = 896155648
[M::mem_process_seqs] Processed 727014 reads in 97.574 CPU sec, 3.148 real sec
sorting block 12, #reads = 1104062 mem = 536878584 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306409, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (219.67, 81.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.20, 144.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 295, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (313.09, 222.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1393)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725756 sequences (100000016 bp) in 1.947 CPU sec, 2.086 real sec...
[M::process] read 724994 sequences (100000156 bp) in 1.728 CPU sec, 1.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 305962, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 158, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (199.04, 91.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.70, 145.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 207, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (225.92, 72.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726398 reads in 168.673 CPU sec, 5.428 real sec
[M::mem_process_seqs] Processed 726764 reads in 97.401 CPU sec, 3.114 real sec
sorting block 13, #reads = 1103345 mem = 536878928 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306234, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 206, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (85, 320)
[M::mem_pestat] mean and std.dev: (195.50, 41.36)
[M::mem_pestat] low and high boundaries for proper pairs: (30, 367)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.69, 145.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 215, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (202.11, 69.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724662 sequences (100000221 bp) in 2.253 CPU sec, 2.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305535, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 223, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (292.67, 182.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 724658 sequences (100000095 bp) in 1.710 CPU sec, 1.715 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (172, 263, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (285.81, 144.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 205, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (185.08, 56.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725756 reads in 166.430 CPU sec, 5.351 real sec
[M::mem_process_seqs] Processed 724994 reads in 95.749 CPU sec, 3.071 real sec
sorting block 14, #reads = 1103247 mem = 536877663 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305825, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 190, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (187.48, 62.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 629)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (288.17, 146.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 180, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 350)
[M::mem_pestat] mean and std.dev: (183.00, 49.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724310 sequences (100000003 bp) in 2.339 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305003, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 180, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 337)
[M::mem_pestat] mean and std.dev: (177.20, 38.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 402)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (288.43, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 226, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (245.89, 127.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724662 reads in 168.531 CPU sec, 5.434 real sec
[M::mem_process_seqs] Processed 724658 reads in 96.112 CPU sec, 3.072 real sec
[M::process] read 724654 sequences (100000217 bp) in 1.820 CPU sec, 1.848 real sec...
sorting block 15, #reads = 1102331 mem = 536879456 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305025, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 143, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413)
[M::mem_pestat] mean and std.dev: (161.31, 46.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 509)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.31, 147.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 342, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (279.86, 82.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1101822 mem = 536879765 peak = 896155648
[M::process] read 724458 sequences (100000024 bp) in 2.442 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305620, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 171, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (185.30, 51.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 509)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (289.07, 146.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 189, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (191.24, 65.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724230 sequences (100000179 bp) in 1.690 CPU sec, 1.694 real sec...
[M::mem_process_seqs] Processed 724310 reads in 166.355 CPU sec, 5.369 real sec
[M::mem_process_seqs] Processed 724654 reads in 95.925 CPU sec, 3.093 real sec
sorting block 17, #reads = 1101592 mem = 536876278 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305158, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 133, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (166.38, 74.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 547)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (289.32, 145.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 724252 sequences (100000165 bp) in 2.318 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305147, 9, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 229, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (212.88, 69.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (290.20, 146.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 134, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482)
[M::mem_pestat] mean and std.dev: (156.76, 61.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 602)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724458 reads in 168.398 CPU sec, 5.445 real sec
[M::process] read 723630 sequences (100000233 bp) in 1.775 CPU sec, 1.788 real sec...
[M::mem_process_seqs] Processed 724230 reads in 97.457 CPU sec, 3.158 real sec
sorting block 18, #reads = 1101932 mem = 536881568 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305410, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 334)
[M::mem_pestat] mean and std.dev: (159.88, 48.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (291.22, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 258, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (229.50, 77.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723372 sequences (100000117 bp) in 2.200 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304816, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 189, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (191.88, 69.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (293.58, 148.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (203.12, 65.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723490 sequences (100000186 bp) in 1.754 CPU sec, 1.762 real sec...
[M::mem_process_seqs] Processed 724252 reads in 168.265 CPU sec, 5.420 real sec
[M::mem_process_seqs] Processed 723630 reads in 97.262 CPU sec, 3.121 real sec
sorting block 19, #reads = 1101312 mem = 536878152 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305067, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 189, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (244.23, 139.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 274, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (296.71, 151.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 257, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (212.93, 83.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 20, #reads = 1101246 mem = 536877750 peak = 896155648
[M::process] read 722986 sequences (100000156 bp) in 3.327 CPU sec, 3.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 304874, 5, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 230, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 395)
[M::mem_pestat] mean and std.dev: (216.41, 74.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 274, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (295.80, 150.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 181, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (214.37, 91.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723372 reads in 171.205 CPU sec, 5.539 real sec
[M::mem_process_seqs] Processed 723490 reads in 100.000 CPU sec, 3.220 real sec
[M::process] read 724428 sequences (100000287 bp) in 2.254 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304441, 9, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 182, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (187.62, 51.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 275, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (296.69, 149.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 171, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 270)
[M::mem_pestat] mean and std.dev: (145.88, 29.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 315)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 21, #reads = 1100727 mem = 536881134 peak = 896155648
[M::process] read 727746 sequences (100000110 bp) in 2.540 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305454, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 184, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (192.42, 72.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (291.62, 148.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 181, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (199.89, 102.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722986 reads in 170.067 CPU sec, 5.468 real sec
[M::mem_process_seqs] Processed 724428 reads in 100.078 CPU sec, 3.188 real sec
[M::process] read 727278 sequences (100000066 bp) in 1.941 CPU sec, 2.028 real sec...
sorting block 22, #reads = 1100741 mem = 536879325 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 307020, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 190, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461)
[M::mem_pestat] mean and std.dev: (196.25, 60.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.05, 143.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 149, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (169.07, 63.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 505)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726874 sequences (100000112 bp) in 2.729 CPU sec, 3.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306376, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 272, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (224.23, 71.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.91, 142.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 131, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (208.38, 132.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727746 reads in 171.335 CPU sec, 5.511 real sec
sorting block 23, #reads = 1102111 mem = 536879193 peak = 896155648
[M::process] read 726302 sequences (100000277 bp) in 2.358 CPU sec, 2.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306310, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 174, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 343)
[M::mem_pestat] mean and std.dev: (178.42, 56.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 406)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.37, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 161, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (170.70, 64.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726874 reads in 92.118 CPU sec, 2.944 real sec
[M::process] read 725848 sequences (100000185 bp) in 1.877 CPU sec, 1.969 real sec...
sorting block 24, #reads = 1103743 mem = 536876425 peak = 896155648
[M::mem_process_seqs] Processed 727278 reads in 248.408 CPU sec, 7.952 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306779, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 170, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (180.45, 67.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.57, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 232, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (221.12, 82.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725234 sequences (100000149 bp) in 2.631 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305925, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 188, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (200.06, 91.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.10, 145.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 151, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (168.09, 85.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726302 reads in 178.948 CPU sec, 5.733 real sec
[M::mem_process_seqs] Processed 725848 reads in 100.095 CPU sec, 3.208 real sec
sorting block 25, #reads = 1103792 mem = 536880588 peak = 896155648
[M::process] read 724896 sequences (100000276 bp) in 1.991 CPU sec, 2.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305870, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 238, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (256.11, 82.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (284.36, 143.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 164, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (191.69, 110.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 26, #reads = 1103010 mem = 536881432 peak = 896155648
[M::process] read 724950 sequences (100000162 bp) in 2.965 CPU sec, 3.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305228, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 220, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (226.13, 97.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (287.32, 146.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 215, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (205.04, 78.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725234 reads in 169.951 CPU sec, 5.459 real sec
[M::mem_process_seqs] Processed 724896 reads in 98.863 CPU sec, 3.144 real sec
[M::process] read 724728 sequences (100000129 bp) in 2.414 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305651, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 206, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (219.10, 76.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 527)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (288.48, 146.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 27, #reads = 1102449 mem = 536880527 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305538, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 167, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (177.67, 71.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (289.80, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 167, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (180.59, 62.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724476 sequences (100000252 bp) in 3.106 CPU sec, 3.459 real sec...
[M::mem_process_seqs] Processed 724950 reads in 168.859 CPU sec, 5.455 real sec
[M::mem_process_seqs] Processed 724728 reads in 99.275 CPU sec, 3.189 real sec
sorting block 28, #reads = 1101763 mem = 536877371 peak = 896155648
[M::process] read 724714 sequences (100000094 bp) in 2.732 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305241, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 186, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (171.20, 45.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (289.45, 146.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 248, 1333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3643)
[M::mem_pestat] mean and std.dev: (624.44, 916.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4798)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305444, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 209, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (231.60, 116.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.54, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 248, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (225.05, 73.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724030 sequences (100000193 bp) in 2.550 CPU sec, 2.785 real sec...
[M::mem_process_seqs] Processed 724476 reads in 169.872 CPU sec, 5.481 real sec
[M::mem_process_seqs] Processed 724714 reads in 97.043 CPU sec, 3.098 real sec
sorting block 29, #reads = 1101919 mem = 536877873 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305250, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 225, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (212.11, 76.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (291.87, 147.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 199, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (210.91, 85.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724030 reads in 90.345 CPU sec, 3.060 real sec
[M::process] read 724522 sequences (100000022 bp) in 3.216 CPU sec, 3.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305446, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 168, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372)
[M::mem_pestat] mean and std.dev: (171.08, 35.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (291.22, 148.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 196, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (208.73, 114.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724522 reads in 87.016 CPU sec, 2.927 real sec
sorting block 30, #reads = 1102302 mem = 536879641 peak = 896155648
[M::process] read 723716 sequences (100000011 bp) in 3.233 CPU sec, 3.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304406, 8, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 187, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (178.46, 49.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (293.09, 149.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 200, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313)
[M::mem_pestat] mean and std.dev: (333.00, 340.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1701)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724014 sequences (100000116 bp) in 2.777 CPU sec, 3.039 real sec...
[M::mem_process_seqs] Processed 723716 reads in 91.402 CPU sec, 3.138 real sec
sorting block 31, #reads = 1101337 mem = 536879769 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304992, 9, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 170, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 379)
[M::mem_pestat] mean and std.dev: (190.21, 54.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (292.51, 148.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (204.05, 55.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724014 reads in 88.702 CPU sec, 2.946 real sec
[M::process] read 723444 sequences (100000056 bp) in 2.892 CPU sec, 3.177 real sec...
sorting block 32, #reads = 1101179 mem = 536879749 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304605, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 311, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (249.88, 149.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 273, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (295.25, 149.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 196, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (203.00, 90.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723444 reads in 89.601 CPU sec, 2.978 real sec
[M::process] read 722814 sequences (100000138 bp) in 3.187 CPU sec, 3.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304609, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 202, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (176.65, 67.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 275, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (297.16, 150.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 180, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (185.61, 81.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722814 reads in 87.739 CPU sec, 2.925 real sec
sorting block 33, #reads = 1101041 mem = 536880539 peak = 896155648
[M::process] read 725596 sequences (100000123 bp) in 2.920 CPU sec, 3.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306010, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 219, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (81, 326)
[M::mem_pestat] mean and std.dev: (192.25, 40.29)
[M::mem_pestat] low and high boundaries for proper pairs: (31, 375)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (286.95, 146.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 254, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (216.23, 79.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725596 reads in 89.040 CPU sec, 2.987 real sec
[M::process] read 727582 sequences (100000247 bp) in 2.714 CPU sec, 3.106 real sec...
sorting block 34, #reads = 1100308 mem = 536878651 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306470, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 191, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 361)
[M::mem_pestat] mean and std.dev: (183.35, 59.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 432)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (279.76, 143.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 198, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (180.75, 51.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727582 reads in 91.582 CPU sec, 3.025 real sec
[M::process] read 726792 sequences (100000091 bp) in 2.800 CPU sec, 3.071 real sec...
sorting block 35, #reads = 1102616 mem = 536878913 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306541, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 182, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507)
[M::mem_pestat] mean and std.dev: (182.93, 60.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (281.53, 144.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 195, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (193.80, 78.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726792 reads in 88.268 CPU sec, 2.894 real sec
[M::process] read 726428 sequences (100000059 bp) in 2.960 CPU sec, 3.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306303, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (211.33, 95.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.30, 144.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 267, 1399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3861)
[M::mem_pestat] mean and std.dev: (584.13, 673.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5092)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726428 reads in 88.994 CPU sec, 2.926 real sec
sorting block 36, #reads = 1103712 mem = 536877998 peak = 896155648
[M::process] read 726416 sequences (100000252 bp) in 2.790 CPU sec, 3.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306923, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 176, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (184.06, 54.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 452)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.51, 144.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 232, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (219.24, 66.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 688)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726416 reads in 90.291 CPU sec, 2.937 real sec
[M::process] read 725666 sequences (100000214 bp) in 2.801 CPU sec, 3.128 real sec...
sorting block 37, #reads = 1103691 mem = 536878673 peak = 896155648
[M::process] read 725316 sequences (100000009 bp) in 2.276 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305683, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 188, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353)
[M::mem_pestat] mean and std.dev: (165.11, 37.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.66, 146.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 214, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (213.06, 57.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724964 sequences (100000110 bp) in 1.800 CPU sec, 1.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305606, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 231, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (201.26, 75.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (285.37, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 184, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (30, 375)
[M::mem_pestat] mean and std.dev: (193.54, 50.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725666 reads in 166.733 CPU sec, 5.298 real sec
[M::mem_process_seqs] Processed 725316 reads in 96.298 CPU sec, 3.038 real sec
[M::process] read 724684 sequences (100000046 bp) in 2.073 CPU sec, 2.152 real sec...
sorting block 38, #reads = 1103141 mem = 536878969 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305269, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 258, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (264.19, 138.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (287.38, 145.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 161, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (174.54, 50.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724408 sequences (100000264 bp) in 2.829 CPU sec, 3.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305365, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 175, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410)
[M::mem_pestat] mean and std.dev: (175.94, 63.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (288.35, 146.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 241, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (229.57, 103.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724964 reads in 167.903 CPU sec, 5.422 real sec
[M::mem_process_seqs] Processed 724684 reads in 97.393 CPU sec, 3.107 real sec
sorting block 39, #reads = 1102210 mem = 536879579 peak = 896155648
[M::process] read 723980 sequences (100000266 bp) in 2.538 CPU sec, 2.748 real sec...
sorting block 40, #reads = 1102058 mem = 536879094 peak = 896155648
[M::process] read 723964 sequences (100000151 bp) in 2.511 CPU sec, 2.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305040, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 170, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (213.55, 115.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (290.87, 147.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 152, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (173.81, 60.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 547)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723980 reads in 92.873 CPU sec, 2.970 real sec
[M::process] read 723776 sequences (100000222 bp) in 2.720 CPU sec, 2.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305425, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 125, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (144.67, 60.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (289.16, 146.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 210, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (246.17, 182.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304906, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 207, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (189.67, 60.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 270, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (292.97, 148.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 191, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (177.00, 49.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724408 reads in 260.457 CPU sec, 8.369 real sec
[M::mem_process_seqs] Processed 723964 reads in 107.949 CPU sec, 3.508 real sec
sorting block 41, #reads = 1101677 mem = 536879402 peak = 896155648
[M::process] read 723824 sequences (100000274 bp) in 2.666 CPU sec, 2.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 304983, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 210, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 411)
[M::mem_pestat] mean and std.dev: (215.10, 59.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (293.57, 149.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 259, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (258.50, 136.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 42, #reads = 1101366 mem = 536878738 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304924, 8, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 204, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (218.40, 79.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 269, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (291.08, 146.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 258, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 437)
[M::mem_pestat] mean and std.dev: (208.76, 50.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723562 sequences (100000005 bp) in 2.584 CPU sec, 2.786 real sec...
[M::mem_process_seqs] Processed 723776 reads in 165.221 CPU sec, 5.327 real sec
[M::mem_process_seqs] Processed 723824 reads in 97.238 CPU sec, 3.102 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305188, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 226, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (207.00, 85.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 272, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (293.42, 147.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 723314 sequences (100000127 bp) in 3.053 CPU sec, 3.385 real sec...
[M::mem_process_seqs] Processed 723562 reads in 90.169 CPU sec, 3.013 real sec
sorting block 43, #reads = 1100939 mem = 536880423 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 304784, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 160, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (184.88, 53.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 273, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (294.88, 149.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 154, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 322)
[M::mem_pestat] mean and std.dev: (168.62, 43.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723314 reads in 89.069 CPU sec, 3.067 real sec
[M::process] read 723148 sequences (100000061 bp) in 3.100 CPU sec, 3.399 real sec...
sorting block 44, #reads = 1101152 mem = 536877268 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 304440, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 179, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 327)
[M::mem_pestat] mean and std.dev: (159.70, 55.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 385)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (296.09, 149.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 175, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 377)
[M::mem_pestat] mean and std.dev: (186.60, 50.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723148 reads in 89.228 CPU sec, 3.034 real sec
[M::process] read 722624 sequences (100000014 bp) in 2.856 CPU sec, 3.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304464, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 182, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (188.60, 58.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 276, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (298.24, 150.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 168, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (195.06, 104.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722624 reads in 89.695 CPU sec, 3.030 real sec
[M::process] read 727226 sequences (100000270 bp) in 2.896 CPU sec, 3.134 real sec...
sorting block 45, #reads = 1100924 mem = 536878455 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306823, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 189, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (283.06, 237.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (284.22, 147.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (108, 237, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (204.61, 82.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727226 reads in 92.028 CPU sec, 3.124 real sec
[M::process] read 727874 sequences (100000191 bp) in 2.894 CPU sec, 3.215 real sec...
sorting block 46, #reads = 1100062 mem = 536879122 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306969, 0, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 156, 170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 270)
[M::mem_pestat] mean and std.dev: (130.50, 29.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 320)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 256, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (281.54, 146.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 191, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (212.81, 80.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727874 reads in 91.553 CPU sec, 3.062 real sec
[M::process] read 726944 sequences (100000254 bp) in 3.145 CPU sec, 3.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306118, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (170.38, 53.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 259, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (284.21, 147.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 214, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (241.70, 149.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726944 reads in 92.107 CPU sec, 3.134 real sec
[M::process] read 726652 sequences (100000260 bp) in 2.881 CPU sec, 3.206 real sec...
sorting block 47, #reads = 1104078 mem = 536878748 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306485, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (284.28, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 178, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 339)
[M::mem_pestat] mean and std.dev: (187.45, 46.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] skip orientation RR
[M::process] read 725728 sequences (100000209 bp) in 2.714 CPU sec, 2.988 real sec...
[M::mem_process_seqs] Processed 726652 reads in 91.663 CPU sec, 3.095 real sec
sorting block 48, #reads = 1103715 mem = 536877747 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306145, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 171, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (168.69, 65.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 521)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (283.16, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 192, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (198.00, 60.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726014 sequences (100000137 bp) in 2.915 CPU sec, 3.173 real sec...
[M::mem_process_seqs] Processed 725728 reads in 92.824 CPU sec, 3.091 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306214, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 169, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (178.06, 56.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 260, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (282.64, 143.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (101, 160, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (170.85, 78.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726160 sequences (100000193 bp) in 2.719 CPU sec, 2.908 real sec...
[M::mem_process_seqs] Processed 726014 reads in 92.702 CPU sec, 3.115 real sec
sorting block 49, #reads = 1102753 mem = 536881393 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306241, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 230, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (73, 363)
[M::mem_pestat] mean and std.dev: (220.67, 72.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (283.19, 144.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 491)
[M::mem_pestat] mean and std.dev: (237.80, 75.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725312 sequences (100000225 bp) in 2.748 CPU sec, 3.100 real sec...
[M::mem_process_seqs] Processed 726160 reads in 92.163 CPU sec, 3.061 real sec
sorting block 50, #reads = 1103120 mem = 536876464 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305710, 1, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 261, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (210.58, 106.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (286.88, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 196, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507)
[M::mem_pestat] mean and std.dev: (197.59, 81.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725148 sequences (100000022 bp) in 2.901 CPU sec, 3.168 real sec...
[M::mem_process_seqs] Processed 725312 reads in 92.220 CPU sec, 3.100 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305607, 8, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 170, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402)
[M::mem_pestat] mean and std.dev: (181.31, 47.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (287.97, 146.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 181, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 327)
[M::mem_pestat] mean and std.dev: (183.07, 49.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 388)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725058 sequences (100000085 bp) in 2.839 CPU sec, 3.029 real sec...
[M::mem_process_seqs] Processed 725148 reads in 92.276 CPU sec, 3.117 real sec
sorting block 51, #reads = 1103136 mem = 536878897 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305357, 0, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 233, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (219.28, 119.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 264, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (287.26, 145.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 188, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (221.71, 91.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724896 sequences (100000112 bp) in 2.885 CPU sec, 3.095 real sec...
[M::mem_process_seqs] Processed 725058 reads in 91.738 CPU sec, 3.143 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305271, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 228, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (235.27, 79.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 264, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (287.45, 146.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 163, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (194.05, 73.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 52, #reads = 1101850 mem = 536878497 peak = 896155648
[M::process] read 724794 sequences (100000049 bp) in 2.903 CPU sec, 3.181 real sec...
[M::mem_process_seqs] Processed 724896 reads in 93.914 CPU sec, 3.165 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305854, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 216, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431)
[M::mem_pestat] mean and std.dev: (208.18, 53.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (290.02, 148.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (179.25, 56.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724660 sequences (100000281 bp) in 2.939 CPU sec, 3.179 real sec...
[M::mem_process_seqs] Processed 724794 reads in 91.665 CPU sec, 3.074 real sec
sorting block 53, #reads = 1101942 mem = 536878190 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305398, 8, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 254, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (264.50, 97.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (288.92, 147.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 215, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474)
[M::mem_pestat] mean and std.dev: (184.55, 54.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724190 sequences (100000049 bp) in 2.769 CPU sec, 2.992 real sec...
[M::mem_process_seqs] Processed 724660 reads in 92.730 CPU sec, 3.170 real sec
[M::process] read 724448 sequences (100000281 bp) in 2.441 CPU sec, 2.597 real sec...
sorting block 54, #reads = 1102193 mem = 536878754 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 305491, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 178, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453)
[M::mem_pestat] mean and std.dev: (187.52, 60.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 554)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (290.87, 148.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 230, 1410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3942)
[M::mem_pestat] mean and std.dev: (574.53, 861.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5208)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724124 sequences (100000100 bp) in 2.857 CPU sec, 3.203 real sec...
[M::mem_process_seqs] Processed 724448 reads in 94.583 CPU sec, 3.098 real sec
sorting block 55, #reads = 1101787 mem = 536878304 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305497, 3, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 181, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (183.62, 65.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 271, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (295.26, 151.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 199, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (184.25, 55.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723680 sequences (100000172 bp) in 2.806 CPU sec, 2.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 304654, 9, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 190, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (227.08, 96.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_process_seqs] Processed 724124 reads in 93.019 CPU sec, 3.071 real sec
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (291.53, 149.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 247, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (213.15, 76.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 650)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305106, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 202, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (240.11, 145.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 273, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (297.19, 152.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 199, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (176.38, 68.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725410 sequences (100000182 bp) in 2.653 CPU sec, 2.932 real sec...
[M::mem_process_seqs] Processed 724190 reads in 362.447 CPU sec, 12.099 real sec
[M::mem_process_seqs] Processed 723680 reads in 101.500 CPU sec, 3.280 real sec
sorting block 56, #reads = 1101266 mem = 536881729 peak = 896155648
[M::process] read 728776 sequences (100000245 bp) in 2.821 CPU sec, 3.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305407, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 201, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (202.31, 75.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 265, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (291.57, 152.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 219, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (189.40, 72.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 57, #reads = 1100861 mem = 536878336 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 307425, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 183, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (195.46, 84.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 253, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (278.25, 144.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 168, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372)
[M::mem_pestat] mean and std.dev: (174.88, 55.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728446 sequences (100000255 bp) in 2.685 CPU sec, 2.899 real sec...
[M::mem_process_seqs] Processed 725410 reads in 176.956 CPU sec, 5.733 real sec
[M::mem_process_seqs] Processed 728776 reads in 101.735 CPU sec, 3.282 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307074, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 176, 1101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3017)
[M::mem_pestat] mean and std.dev: (250.73, 273.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3975)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (279.84, 145.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 203, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (215.93, 76.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727694 sequences (100000154 bp) in 2.888 CPU sec, 3.116 real sec...
[M::mem_process_seqs] Processed 728446 reads in 91.830 CPU sec, 3.084 real sec
sorting block 58, #reads = 1102098 mem = 536877936 peak = 896155648
[M::process] read 726904 sequences (100000165 bp) in 2.236 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307067, 4, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 165, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (176.41, 64.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (281.52, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 171, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (198.24, 95.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 59, #reads = 1105283 mem = 536880593 peak = 896155648
[M::process] read 726422 sequences (100000065 bp) in 2.783 CPU sec, 2.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306502, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 207, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (217.50, 66.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.97, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 234, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (207.07, 69.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727694 reads in 172.162 CPU sec, 5.558 real sec
[M::mem_process_seqs] Processed 726904 reads in 100.417 CPU sec, 3.231 real sec
[M::process] read 726388 sequences (100000243 bp) in 2.930 CPU sec, 3.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306240, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (232.24, 112.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (282.19, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 210, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (244.00, 108.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 60, #reads = 1104354 mem = 536880449 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306237, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 226, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (213.00, 64.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.46, 143.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 271, 725)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827)
[M::mem_pestat] mean and std.dev: (374.94, 261.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2378)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726422 reads in 175.107 CPU sec, 5.661 real sec
[M::process] read 725706 sequences (100000227 bp) in 2.761 CPU sec, 3.202 real sec...
[M::mem_process_seqs] Processed 726388 reads in 101.011 CPU sec, 3.247 real sec
sorting block 61, #reads = 1103878 mem = 536879121 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305965, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 199, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 420)
[M::mem_pestat] mean and std.dev: (215.82, 85.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.34, 145.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 233, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 373)
[M::mem_pestat] mean and std.dev: (200.62, 61.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725722 sequences (100000266 bp) in 2.891 CPU sec, 3.095 real sec...
[M::mem_process_seqs] Processed 725706 reads in 93.718 CPU sec, 3.164 real sec
[M::process] read 725928 sequences (100000203 bp) in 2.466 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306236, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 164, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (212.21, 115.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (285.77, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 251, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (316.95, 178.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 62, #reads = 1103119 mem = 536879741 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305705, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 161, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517)
[M::mem_pestat] mean and std.dev: (189.94, 109.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (285.35, 146.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 219, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (235.67, 83.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725444 sequences (100000055 bp) in 2.676 CPU sec, 2.950 real sec...
[M::mem_process_seqs] Processed 725722 reads in 172.709 CPU sec, 5.646 real sec
[M::mem_process_seqs] Processed 725928 reads in 99.899 CPU sec, 3.318 real sec
sorting block 63, #reads = 1102468 mem = 536879559 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305804, 2, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 184, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 260)
[M::mem_pestat] mean and std.dev: (174.90, 32.92)
[M::mem_pestat] low and high boundaries for proper pairs: (43, 307)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (287.75, 147.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 239, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 416)
[M::mem_pestat] mean and std.dev: (221.24, 62.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725530 sequences (100000049 bp) in 2.836 CPU sec, 3.116 real sec...
[M::mem_process_seqs] Processed 725444 reads in 89.736 CPU sec, 3.054 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306543, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 178, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.88, 109.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (287.65, 148.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 254, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (215.90, 95.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725506 sequences (100000125 bp) in 2.731 CPU sec, 2.949 real sec...
[M::mem_process_seqs] Processed 725530 reads in 90.441 CPU sec, 3.043 real sec
sorting block 64, #reads = 1101972 mem = 536880977 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305808, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 300, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (251.50, 86.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 659)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (287.60, 147.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 269, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (255.62, 109.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725368 sequences (100000004 bp) in 2.784 CPU sec, 3.024 real sec...
[M::mem_process_seqs] Processed 725506 reads in 92.592 CPU sec, 3.194 real sec
sorting block 65, #reads = 1102862 mem = 536878651 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305713, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 160, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (183.64, 100.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (288.86, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 239, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (374.89, 306.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1600)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724826 sequences (100000045 bp) in 2.798 CPU sec, 3.084 real sec...
[M::mem_process_seqs] Processed 725368 reads in 91.486 CPU sec, 3.110 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305417, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 184, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (177.25, 50.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (290.79, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 724858 sequences (100000149 bp) in 2.701 CPU sec, 3.087 real sec...
[M::mem_process_seqs] Processed 724826 reads in 92.887 CPU sec, 3.181 real sec
sorting block 66, #reads = 1102612 mem = 536880570 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305794, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 197, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 341)
[M::mem_pestat] mean and std.dev: (185.69, 37.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (293.44, 152.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 172, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377)
[M::mem_pestat] mean and std.dev: (182.40, 68.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724774 sequences (100000040 bp) in 2.688 CPU sec, 2.956 real sec...
[M::mem_process_seqs] Processed 724858 reads in 91.482 CPU sec, 3.110 real sec
sorting block 67, #reads = 1102176 mem = 536876996 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305646, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 223, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (199.27, 69.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (293.40, 151.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 230, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (248.41, 99.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724774 reads in 92.051 CPU sec, 3.097 real sec
[M::process] read 725232 sequences (100000232 bp) in 2.908 CPU sec, 3.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305551, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 166, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365)
[M::mem_pestat] mean and std.dev: (179.06, 50.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 438)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (293.45, 153.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 170, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (198.23, 73.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728900 sequences (100000115 bp) in 2.732 CPU sec, 2.985 real sec...
[M::mem_process_seqs] Processed 725232 reads in 93.364 CPU sec, 3.223 real sec
sorting block 68, #reads = 1102281 mem = 536880544 peak = 896155648
[M::process] read 728000 sequences (100000141 bp) in 2.625 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 307302, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 216, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (259.50, 179.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 252, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (277.70, 144.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 199, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (201.78, 55.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728900 reads in 92.712 CPU sec, 3.118 real sec
sorting block 69, #reads = 1101193 mem = 536880682 peak = 896155648
[M::process] read 727760 sequences (100000007 bp) in 2.764 CPU sec, 2.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306509, 9, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 221, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 385)
[M::mem_pestat] mean and std.dev: (196.00, 53.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (279.89, 144.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 223, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (250.00, 150.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306246, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 155, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 334)
[M::mem_pestat] mean and std.dev: (168.94, 51.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (280.45, 145.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 270, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (252.95, 142.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727224 sequences (100000167 bp) in 2.707 CPU sec, 2.897 real sec...
[M::mem_process_seqs] Processed 728000 reads in 176.494 CPU sec, 5.748 real sec
[M::mem_process_seqs] Processed 727760 reads in 101.157 CPU sec, 3.266 real sec
sorting block 70, #reads = 1105035 mem = 536880847 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306514, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 256, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (234.12, 96.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 726852 sequences (100000005 bp) in 2.709 CPU sec, 2.935 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (166, 257, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (282.26, 146.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 180, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432)
[M::mem_pestat] mean and std.dev: (176.50, 48.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306237, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 232, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (206.50, 81.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.80, 144.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 223, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166)
[M::mem_pestat] mean and std.dev: (214.50, 87.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1488)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727224 reads in 176.541 CPU sec, 5.745 real sec
[M::mem_process_seqs] Processed 726852 reads in 102.424 CPU sec, 3.300 real sec
sorting block 71, #reads = 1104241 mem = 536878821 peak = 896155648
[M::process] read 726528 sequences (100000230 bp) in 3.363 CPU sec, 3.724 real sec...
[M::process] read 726568 sequences (100000130 bp) in 2.408 CPU sec, 2.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306578, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 270, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436)
[M::mem_pestat] mean and std.dev: (354.88, 200.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1852)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (281.10, 143.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 215, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310)
[M::mem_pestat] mean and std.dev: (237.15, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1690)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 72, #reads = 1103982 mem = 536880666 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306665, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 178, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (186.27, 95.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.44, 144.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 229, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 365)
[M::mem_pestat] mean and std.dev: (204.64, 54.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726528 reads in 178.197 CPU sec, 5.806 real sec
[M::process] read 726436 sequences (100000048 bp) in 2.782 CPU sec, 3.034 real sec...
[M::mem_process_seqs] Processed 726568 reads in 101.755 CPU sec, 3.295 real sec
sorting block 73, #reads = 1103347 mem = 536879109 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306351, 9, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 197, 2530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7336)
[M::mem_pestat] mean and std.dev: (939.47, 1328.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9739)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.84, 145.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (274.88, 185.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725886 sequences (100000189 bp) in 2.730 CPU sec, 3.020 real sec...
[M::mem_process_seqs] Processed 726436 reads in 93.589 CPU sec, 3.176 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306054, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 214, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (229.83, 82.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.74, 145.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 190, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (195.94, 97.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726026 sequences (100000148 bp) in 2.721 CPU sec, 2.932 real sec...
[M::mem_process_seqs] Processed 725886 reads in 91.571 CPU sec, 3.062 real sec
sorting block 74, #reads = 1104094 mem = 536878046 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306402, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 210, 807)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153)
[M::mem_pestat] mean and std.dev: (361.56, 300.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2826)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (286.22, 147.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 255, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (243.06, 110.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726026 reads in 89.086 CPU sec, 2.988 real sec
[M::process] read 725586 sequences (100000153 bp) in 2.992 CPU sec, 3.187 real sec...
sorting block 75, #reads = 1102398 mem = 536877119 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305952, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 254, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (359.86, 258.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1510)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.69, 146.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 219, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (188.00, 79.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725532 sequences (100000153 bp) in 2.861 CPU sec, 3.055 real sec...
[M::mem_process_seqs] Processed 725586 reads in 93.027 CPU sec, 3.177 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305692, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 185, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (198.56, 90.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.70, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (205.00, 72.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725334 sequences (100000173 bp) in 2.698 CPU sec, 2.972 real sec...
[M::mem_process_seqs] Processed 725532 reads in 90.926 CPU sec, 3.064 real sec
sorting block 76, #reads = 1102878 mem = 536879346 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305167, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 214, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (237.93, 123.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (288.47, 148.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 223, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (238.70, 115.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725104 sequences (100000019 bp) in 2.804 CPU sec, 3.020 real sec...
[M::mem_process_seqs] Processed 725334 reads in 92.671 CPU sec, 3.177 real sec
sorting block 77, #reads = 1103067 mem = 536882276 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 305416, 5, 26)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (291.39, 150.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 247, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (233.72, 97.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] skip orientation RR
[M::process] read 724888 sequences (100000193 bp) in 2.891 CPU sec, 3.082 real sec...
[M::mem_process_seqs] Processed 725104 reads in 92.110 CPU sec, 3.077 real sec
[M::process] read 724750 sequences (100000188 bp) in 2.145 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 305292, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 207, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (206.48, 76.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (292.55, 151.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 277, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (233.64, 71.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 78, #reads = 1101959 mem = 536879797 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305152, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 328, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248)
[M::mem_pestat] mean and std.dev: (293.92, 170.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1605)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 724674 sequences (100000074 bp) in 2.940 CPU sec, 3.296 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (293.09, 151.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 325, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (261.50, 106.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724888 reads in 176.560 CPU sec, 5.750 real sec
[M::mem_process_seqs] Processed 724750 reads in 102.390 CPU sec, 3.354 real sec
sorting block 79, #reads = 1102298 mem = 536879417 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305620, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 145, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (163.91, 50.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (294.97, 153.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 193, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (213.83, 73.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729344 sequences (100000053 bp) in 2.742 CPU sec, 3.005 real sec...
[M::mem_process_seqs] Processed 724674 reads in 92.920 CPU sec, 3.131 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307287, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (193.94, 65.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 250, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (277.40, 145.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 254, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (46, 441)
[M::mem_pestat] mean and std.dev: (231.18, 52.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727894 sequences (100000123 bp) in 2.863 CPU sec, 3.163 real sec...
[M::mem_process_seqs] Processed 729344 reads in 93.914 CPU sec, 3.168 real sec
sorting block 80, #reads = 1101412 mem = 536878295 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306417, 3, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 192, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (174.88, 71.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 727286 sequences (100000088 bp) in 2.827 CPU sec, 3.151 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (279.92, 145.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 198, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (215.36, 93.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 306245, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 182, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (226.11, 113.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.25, 145.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 166, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 245)
[M::mem_pestat] mean and std.dev: (152.88, 19.38)
[M::mem_pestat] low and high boundaries for proper pairs: (47, 278)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727894 reads in 178.646 CPU sec, 5.837 real sec
[M::mem_process_seqs] Processed 727286 reads in 103.239 CPU sec, 3.349 real sec
sorting block 81, #reads = 1104182 mem = 536882873 peak = 896155648
[M::process] read 727118 sequences (100000260 bp) in 3.171 CPU sec, 3.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306448, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 190, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (175.29, 64.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 443)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (282.30, 145.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 207, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (208.06, 107.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726608 sequences (100000161 bp) in 2.814 CPU sec, 3.098 real sec...
[M::mem_process_seqs] Processed 727118 reads in 93.071 CPU sec, 3.157 real sec
sorting block 82, #reads = 1103928 mem = 536879691 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306424, 11, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 157, 165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 253)
[M::mem_pestat] mean and std.dev: (144.50, 37.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 297)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (284.47, 146.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 41, 101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275)
[M::mem_pestat] mean and std.dev: (46.33, 39.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 362)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 175, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402)
[M::mem_pestat] mean and std.dev: (178.79, 68.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 726672 sequences (100000227 bp) in 2.878 CPU sec, 3.063 real sec...
[M::mem_process_seqs] Processed 726608 reads in 92.907 CPU sec, 3.157 real sec
sorting block 83, #reads = 1103645 mem = 536877426 peak = 896155648
[M::process] read 726164 sequences (100000113 bp) in 2.319 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306559, 10, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 135, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471)
[M::mem_pestat] mean and std.dev: (169.62, 65.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (284.85, 147.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 91, 126)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236)
[M::mem_pestat] mean and std.dev: (82.22, 33.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 291)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 164, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (185.33, 70.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 725904 sequences (100000271 bp) in 2.028 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305766, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 171, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (189.35, 76.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (283.88, 145.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 172, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (204.58, 84.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726672 reads in 178.410 CPU sec, 5.810 real sec
[M::mem_process_seqs] Processed 726164 reads in 105.323 CPU sec, 3.401 real sec
sorting block 84, #reads = 1103571 mem = 536880041 peak = 896155648
[M::process] read 725568 sequences (100000096 bp) in 3.095 CPU sec, 3.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 305604, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 190, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (240.84, 138.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.50, 145.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 233, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (203.25, 82.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305220, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 169, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 379)
[M::mem_pestat] mean and std.dev: (177.36, 58.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.31, 145.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 153, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361)
[M::mem_pestat] mean and std.dev: (161.14, 37.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725638 sequences (100000011 bp) in 2.710 CPU sec, 2.936 real sec...
sorting block 85, #reads = 1103151 mem = 536880145 peak = 896155648
[M::mem_process_seqs] Processed 725904 reads in 179.361 CPU sec, 5.897 real sec
[M::mem_process_seqs] Processed 725568 reads in 103.912 CPU sec, 3.371 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305944, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 192, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (192.73, 80.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.36, 146.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 252, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (243.10, 84.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725094 sequences (100000057 bp) in 2.780 CPU sec, 3.097 real sec...
[M::mem_process_seqs] Processed 725638 reads in 92.904 CPU sec, 3.216 real sec
sorting block 86, #reads = 1102632 mem = 536877994 peak = 896155648
[M::process] read 725242 sequences (100000004 bp) in 2.486 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 305368, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 188, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (196.10, 85.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 547)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (286.23, 145.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 208, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (188.75, 53.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 459)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305910, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 208, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 365)
[M::mem_pestat] mean and std.dev: (191.47, 60.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 436)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (287.47, 146.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 170, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (205.40, 99.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725094 reads in 174.920 CPU sec, 5.659 real sec
[M::process] read 724700 sequences (100000153 bp) in 2.887 CPU sec, 3.197 real sec...
[M::mem_process_seqs] Processed 725242 reads in 100.667 CPU sec, 3.224 real sec
sorting block 87, #reads = 1102583 mem = 536878454 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305514, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 225, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (265.64, 133.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (289.35, 147.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 222, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (230.33, 103.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724700 reads in 90.400 CPU sec, 3.020 real sec
[M::process] read 724686 sequences (100000216 bp) in 2.847 CPU sec, 3.048 real sec...
sorting block 88, #reads = 1102352 mem = 536877794 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 305484, 3, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 177, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425)
[M::mem_pestat] mean and std.dev: (186.65, 70.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (290.14, 148.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 170, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435)
[M::mem_pestat] mean and std.dev: (166.33, 71.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724686 reads in 89.099 CPU sec, 2.982 real sec
[M::process] read 724542 sequences (100000162 bp) in 2.967 CPU sec, 3.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 304697, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 179, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (222.38, 116.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 89, #reads = 1101910 mem = 536879270 peak = 896155648
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (291.15, 148.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 258, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565)
[M::mem_pestat] mean and std.dev: (232.47, 60.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724542 reads in 91.383 CPU sec, 3.047 real sec
[M::process] read 724420 sequences (100000068 bp) in 2.966 CPU sec, 3.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 305179, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 186, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (226.12, 111.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (293.69, 151.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 192, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495)
[M::mem_pestat] mean and std.dev: (196.31, 68.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724276 sequences (100000134 bp) in 2.838 CPU sec, 3.070 real sec...
[M::mem_process_seqs] Processed 724420 reads in 94.303 CPU sec, 3.149 real sec
[M::process] read 228460 sequences (31537676 bp) in 0.979 CPU sec, 1.015 real sec...
sorting block 90, #reads = 1101454 mem = 536880576 peak = 896155648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 304221, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 169, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 270)
[M::mem_pestat] mean and std.dev: (151.73, 25.43)
[M::mem_pestat] low and high boundaries for proper pairs: (6, 314)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 269, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (294.72, 152.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 211, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (284.15, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 96253, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 269, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (294.32, 151.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 724276 reads in 118.990 CPU sec, 3.936 real sec
[M::mem_process_seqs] Processed 228460 reads in 38.265 CPU sec, 1.265 real sec
sorting block 91, #reads = 1101532 mem = 536876803 peak = 896155648
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 101778170
overall: 69398859776 mem 14306.280 user 768.383 sys 515.470 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.SH032_HG514.L008\tPU:L008\tSM:SH032_HG514\tLB:SH032_HG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.SH032_HG514_R1.fastq.gz 0004.SH032_HG514_R2.fastq.gz
[main] Real time: 515.501 sec; CPU: 15074.667 sec
sorting block 92, #reads = 958925 mem = 467590045 peak = 896155648
uniq header count 1
merging 93 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 4451677792
output reads: 102376902
bam_mem_sort: 93 calls 69.494 user 0.992 sys 71.129 real
bam_write: 93 calls 53.220 user 4.264 sys 85.603 real
execute: 1 calls 30.487 user 2.876 sys 551.025 real
merge_files: 1 calls 30.455 user 2.852 sys 34.681 real
parse_chunk: 1486 calls 69.322 user 19.988 sys 99.492 real
read_chunk: 4023 calls 45.918 user 22.532 sys 440.712 real
sort_block: 1 calls 35.589 user 21.291 sys 425.422 real
write_chunk: 2535 calls 29.901 user 2.600 sys 33.569 real
overall: 2258849792 mem 1170.092 user 77.486 sys 551.045 real
ls: cannot access '*.cram': No such file or directory