File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/32/0049f5bccb2aab0be9e1a3caceb93f/.command.err
Size
192.1 KB
Task
DAQ:SENTIEON_BWAMEM (SH032_HG514;L002;0002)
Attempt

Content

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This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o SH032_HG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2847.492 CPU sec, 108.677 real sec
[M::process] read 728776 sequences (100000094 bp) in 1.376 CPU sec, 1.379 real sec...
[M::process] read 727220 sequences (100000112 bp) in 1.630 CPU sec, 1.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 307147, 1, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 215, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (219.44, 67.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (275.25, 141.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (191.14, 65.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726976 sequences (100000273 bp) in 1.392 CPU sec, 1.406 real sec...
[M::process] read 726390 sequences (100000196 bp) in 1.519 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306694, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 162, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495)
[M::mem_pestat] mean and std.dev: (194.64, 109.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.93, 143.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 154, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349)
[M::mem_pestat] mean and std.dev: (171.40, 68.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728776 reads in 186.606 CPU sec, 6.001 real sec
[M::mem_process_seqs] Processed 727220 reads in 108.877 CPU sec, 3.612 real sec
sorting block 0, #reads = 1104528 mem = 536877792 peak = 592125952
[M::process] read 726304 sequences (100000240 bp) in 2.116 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306540, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 179, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (177.07, 48.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.11, 143.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 165, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420)
[M::mem_pestat] mean and std.dev: (193.81, 91.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 558)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725668 sequences (100000056 bp) in 1.430 CPU sec, 1.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306197, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 136, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310)
[M::mem_pestat] mean and std.dev: (131.85, 32.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 378)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.47, 144.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 152, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (200.69, 129.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726976 reads in 181.912 CPU sec, 5.837 real sec
[M::mem_process_seqs] Processed 726390 reads in 113.607 CPU sec, 3.691 real sec
sorting block 1, #reads = 1103752 mem = 536881489 peak = 749412352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306086, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 207, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (181.33, 50.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.83, 144.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 297, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (271.55, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725482 sequences (100000046 bp) in 2.062 CPU sec, 2.219 real sec...
[M::process] read 725342 sequences (100000276 bp) in 1.257 CPU sec, 1.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306368, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 202, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (218.57, 84.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (285.19, 145.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 214, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (217.22, 79.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726304 reads in 182.988 CPU sec, 5.887 real sec
[M::mem_process_seqs] Processed 725668 reads in 114.331 CPU sec, 3.740 real sec
sorting block 2, #reads = 1103391 mem = 536877994 peak = 791703552
[M::process] read 725824 sequences (100000232 bp) in 1.620 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306139, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 295, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (273.45, 128.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1350)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.52, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 192, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (218.44, 112.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724982 sequences (100000156 bp) in 1.157 CPU sec, 1.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306083, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 159, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (153.15, 46.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.60, 146.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 238, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (218.76, 93.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 305269, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 237, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (44, 459)
[M::mem_pestat] mean and std.dev: (229.96, 65.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.37, 146.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 239, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (341.60, 258.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1375)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725482 reads in 264.494 CPU sec, 8.619 real sec
sorting block 3, #reads = 1103415 mem = 536878079 peak = 792932352
[M::mem_process_seqs] Processed 725824 reads in 194.426 CPU sec, 6.433 real sec
[M::mem_process_seqs] Processed 725342 reads in 209.150 CPU sec, 6.972 real sec
sorting block 4, #reads = 1102785 mem = 536877695 peak = 866156544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305612, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 210, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (206.22, 85.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (286.07, 145.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 232, 1574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4406)
[M::mem_pestat] mean and std.dev: (595.82, 598.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5822)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724848 sequences (100000106 bp) in 2.983 CPU sec, 3.267 real sec...
[M::mem_process_seqs] Processed 724982 reads in 112.043 CPU sec, 3.753 real sec
[M::process] read 724584 sequences (100000082 bp) in 1.552 CPU sec, 1.615 real sec...
sorting block 5, #reads = 1102407 mem = 536877791 peak = 866156544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305643, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 207, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (223.10, 93.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (288.73, 147.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 209, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 426)
[M::mem_pestat] mean and std.dev: (212.60, 67.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 509)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724294 sequences (100000158 bp) in 2.664 CPU sec, 2.867 real sec...
[M::process] read 724382 sequences (100000030 bp) in 1.545 CPU sec, 1.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305487, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 179, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (165.53, 66.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 265, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (288.33, 146.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 186, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (217.81, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 741)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724848 reads in 203.003 CPU sec, 6.495 real sec
[M::mem_process_seqs] Processed 724584 reads in 124.096 CPU sec, 4.007 real sec
sorting block 6, #reads = 1102429 mem = 536878235 peak = 866156544
[M::process] read 724418 sequences (100000133 bp) in 1.793 CPU sec, 1.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305074, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 218, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (209.68, 79.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (289.52, 146.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 173, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (197.76, 89.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724146 sequences (100000050 bp) in 1.550 CPU sec, 1.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305470, 9, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (184.75, 69.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (289.59, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 177, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (182.43, 58.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305344, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 195, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447)
[M::mem_pestat] mean and std.dev: (191.22, 60.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (291.13, 148.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 170, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (207.69, 75.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724294 reads in 282.669 CPU sec, 9.147 real sec
sorting block 7, #reads = 1102016 mem = 536879168 peak = 866156544
[M::mem_process_seqs] Processed 724382 reads in 204.149 CPU sec, 6.690 real sec
[M::mem_process_seqs] Processed 724418 reads in 218.876 CPU sec, 7.254 real sec
sorting block 8, #reads = 1101557 mem = 536877800 peak = 893501440
[M::process] read 723244 sequences (100000109 bp) in 2.561 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304812, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 179, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (98, 273)
[M::mem_pestat] mean and std.dev: (182.78, 36.63)
[M::mem_pestat] low and high boundaries for proper pairs: (36, 329)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (293.00, 149.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 302, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959)
[M::mem_pestat] mean and std.dev: (286.70, 183.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2547)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725106 sequences (100000169 bp) in 2.241 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304540, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 224, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (209.82, 68.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 629)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 273, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (295.08, 149.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 205, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (188.71, 68.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728492 sequences (100000152 bp) in 1.342 CPU sec, 1.356 real sec...
[M::mem_process_seqs] Processed 724146 reads in 210.628 CPU sec, 6.756 real sec
sorting block 9, #reads = 1101904 mem = 536879469 peak = 893501440
[M::mem_process_seqs] Processed 723244 reads in 122.818 CPU sec, 3.936 real sec
sorting block 10, #reads = 1101011 mem = 536878581 peak = 893501440
[M::process] read 727404 sequences (100000142 bp) in 2.100 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305533, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 191, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (175.46, 110.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (288.90, 147.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 233, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (218.55, 64.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727014 sequences (100000130 bp) in 1.559 CPU sec, 1.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 307283, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 203, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (211.65, 91.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.70, 141.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (102, 188, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453)
[M::mem_pestat] mean and std.dev: (162.67, 69.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306968, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 151, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (159.90, 70.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (278.99, 142.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 161, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (156.53, 56.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 525)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725106 reads in 298.602 CPU sec, 9.615 real sec
[M::mem_process_seqs] Processed 728492 reads in 214.648 CPU sec, 6.978 real sec
[M::mem_process_seqs] Processed 727404 reads in 231.201 CPU sec, 7.583 real sec
sorting block 11, #reads = 1101780 mem = 536876375 peak = 893501440
[M::process] read 726512 sequences (100000185 bp) in 2.307 CPU sec, 2.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306749, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 242, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (212.31, 72.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.67, 142.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 215, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (213.11, 81.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 12, #reads = 1104615 mem = 536877905 peak = 893501440
[M::process] read 726254 sequences (100000173 bp) in 2.869 CPU sec, 3.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306522, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 160, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (178.33, 60.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (281.10, 143.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 174, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (171.48, 55.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727014 reads in 203.258 CPU sec, 6.532 real sec
[M::mem_process_seqs] Processed 726512 reads in 125.894 CPU sec, 4.036 real sec
[M::process] read 725904 sequences (100000180 bp) in 1.692 CPU sec, 1.753 real sec...
sorting block 13, #reads = 1103952 mem = 536880589 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306046, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 225, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (283.90, 172.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (282.21, 143.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 240, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (218.92, 76.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725782 sequences (100000188 bp) in 2.587 CPU sec, 2.807 real sec...
[M::process] read 727294 sequences (100000058 bp) in 1.208 CPU sec, 1.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306059, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 180, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417)
[M::mem_pestat] mean and std.dev: (168.47, 48.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 509)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.85, 144.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 181, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407)
[M::mem_pestat] mean and std.dev: (185.29, 68.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726254 reads in 196.950 CPU sec, 6.325 real sec
[M::mem_process_seqs] Processed 725904 reads in 123.454 CPU sec, 3.998 real sec
sorting block 14, #reads = 1103974 mem = 536881747 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306366, 2, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 296, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305)
[M::mem_pestat] mean and std.dev: (376.50, 314.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3016)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (284.92, 145.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (217.73, 77.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726418 sequences (100000050 bp) in 2.064 CPU sec, 2.229 real sec...
[M::process] read 725574 sequences (100000098 bp) in 1.181 CPU sec, 1.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306820, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 245, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (242.58, 88.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.82, 142.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 178, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (198.50, 86.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725782 reads in 195.308 CPU sec, 6.298 real sec
sorting block 15, #reads = 1102814 mem = 536881599 peak = 893501440
[M::mem_process_seqs] Processed 727294 reads in 122.886 CPU sec, 3.984 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305969, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 166, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (56, 281)
[M::mem_pestat] mean and std.dev: (164.87, 29.08)
[M::mem_pestat] low and high boundaries for proper pairs: (11, 326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.78, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 16, #reads = 1103621 mem = 536879698 peak = 893501440
[M::process] read 725206 sequences (100000101 bp) in 2.194 CPU sec, 2.393 real sec...
[M::process] read 724538 sequences (100000215 bp) in 1.276 CPU sec, 1.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305820, 8, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 177, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (211.20, 88.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (284.49, 145.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 158, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (199.93, 106.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726418 reads in 185.721 CPU sec, 5.979 real sec
[M::mem_process_seqs] Processed 725574 reads in 112.621 CPU sec, 3.664 real sec
sorting block 17, #reads = 1103446 mem = 536878114 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305621, 12, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 175, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (192.94, 91.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 264, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (287.88, 146.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 70, 113)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255)
[M::mem_pestat] mean and std.dev: (60.70, 27.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 326)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 152, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (185.46, 85.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 724160 sequences (100000085 bp) in 2.664 CPU sec, 2.905 real sec...
[M::process] read 724168 sequences (100000112 bp) in 1.290 CPU sec, 1.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 305208, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (288.61, 145.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 178, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 291)
[M::mem_pestat] mean and std.dev: (179.40, 45.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 360)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725206 reads in 182.290 CPU sec, 5.858 real sec
[M::mem_process_seqs] Processed 724538 reads in 107.884 CPU sec, 3.463 real sec
sorting block 18, #reads = 1102397 mem = 536877919 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304722, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 208, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507)
[M::mem_pestat] mean and std.dev: (214.05, 81.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (291.32, 147.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 204, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367)
[M::mem_pestat] mean and std.dev: (221.89, 133.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1776)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723652 sequences (100000127 bp) in 2.671 CPU sec, 2.857 real sec...
[M::process] read 723598 sequences (100000121 bp) in 1.488 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304973, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 253, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 418)
[M::mem_pestat] mean and std.dev: (241.71, 48.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.84, 147.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 278, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (234, 329)
[M::mem_pestat] mean and std.dev: (280.14, 7.24)
[M::mem_pestat] low and high boundaries for proper pairs: (215, 348)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724160 reads in 189.049 CPU sec, 6.073 real sec
sorting block 19, #reads = 1102224 mem = 536879527 peak = 893501440
[M::mem_process_seqs] Processed 724168 reads in 113.581 CPU sec, 3.642 real sec
sorting block 20, #reads = 1101196 mem = 536878817 peak = 893501440
[M::process] read 723072 sequences (100000104 bp) in 2.207 CPU sec, 2.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 304887, 5, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (293.81, 148.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 150, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (167.65, 72.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 566)
[M::mem_pestat] skip orientation RR
[M::process] read 726464 sequences (100000169 bp) in 1.532 CPU sec, 1.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305069, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 189, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (189.43, 83.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (293.39, 148.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 219, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (204.45, 78.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723652 reads in 191.769 CPU sec, 6.146 real sec
[M::mem_process_seqs] Processed 723598 reads in 113.379 CPU sec, 3.666 real sec
sorting block 21, #reads = 1101002 mem = 536877580 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 304878, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (225.22, 96.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (296.09, 149.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 221, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (216.82, 82.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727332 sequences (100000043 bp) in 3.316 CPU sec, 3.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306333, 6, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 170, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330)
[M::mem_pestat] mean and std.dev: (155.45, 31.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.72, 145.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 290, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (236.37, 105.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723072 reads in 189.449 CPU sec, 6.069 real sec
[M::process] read 726894 sequences (100000183 bp) in 1.430 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 726464 reads in 115.750 CPU sec, 3.712 real sec
sorting block 22, #reads = 1100547 mem = 536877774 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306371, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 156, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (164.05, 67.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.46, 142.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 201, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (196.19, 69.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 597)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726646 sequences (100000248 bp) in 2.463 CPU sec, 2.636 real sec...
[M::process] read 726238 sequences (100000086 bp) in 1.509 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306412, 5, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 157, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (172.00, 73.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (281.37, 143.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 214, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 370)
[M::mem_pestat] mean and std.dev: (199.65, 59.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727332 reads in 187.215 CPU sec, 5.995 real sec
[M::mem_process_seqs] Processed 726894 reads in 111.015 CPU sec, 3.570 real sec
sorting block 23, #reads = 1102823 mem = 536880355 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306409, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 182, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 285)
[M::mem_pestat] mean and std.dev: (172.11, 32.54)
[M::mem_pestat] low and high boundaries for proper pairs: (39, 326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.52, 144.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 169, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (178.33, 73.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1103572 mem = 536884721 peak = 893501440
[M::process] read 726390 sequences (100000022 bp) in 2.475 CPU sec, 2.647 real sec...
[M::process] read 725604 sequences (100000230 bp) in 1.317 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306296, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 220, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (229.25, 95.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.07, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726646 reads in 191.327 CPU sec, 6.154 real sec
[M::mem_process_seqs] Processed 726238 reads in 113.654 CPU sec, 3.660 real sec
sorting block 25, #reads = 1103162 mem = 536878658 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306098, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 190, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (212.25, 94.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (284.70, 145.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 204, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 395)
[M::mem_pestat] mean and std.dev: (199.46, 46.00)
[M::mem_pestat] low and high boundaries for proper pairs: (5, 460)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726568 sequences (100000225 bp) in 2.623 CPU sec, 2.906 real sec...
[M::process] read 728016 sequences (100000269 bp) in 1.289 CPU sec, 1.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305692, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309)
[M::mem_pestat] mean and std.dev: (137.38, 54.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 380)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (284.54, 144.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 200, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (195.78, 59.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726390 reads in 185.638 CPU sec, 5.954 real sec
[M::mem_process_seqs] Processed 725604 reads in 112.461 CPU sec, 3.626 real sec
sorting block 26, #reads = 1103409 mem = 536876893 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306244, 1, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 278, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (233.93, 93.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.35, 144.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 145, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (186.50, 79.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726772 sequences (100000000 bp) in 2.247 CPU sec, 2.372 real sec...
[M::process] read 725850 sequences (100000064 bp) in 1.290 CPU sec, 1.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307042, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 208, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (200.31, 87.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.43, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 187, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461)
[M::mem_pestat] mean and std.dev: (188.83, 62.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 566)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726568 reads in 175.073 CPU sec, 5.610 real sec
[M::mem_process_seqs] Processed 728016 reads in 101.247 CPU sec, 3.259 real sec
sorting block 27, #reads = 1102575 mem = 536882268 peak = 893501440
sorting block 28, #reads = 1103645 mem = 536878304 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 306816, 12, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 171, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371)
[M::mem_pestat] mean and std.dev: (175.78, 57.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 448)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.70, 142.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 77, 99)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197)
[M::mem_pestat] mean and std.dev: (68.73, 25.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 246)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 164, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (168.63, 70.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 725396 sequences (100000079 bp) in 2.301 CPU sec, 2.472 real sec...
[M::process] read 724752 sequences (100000010 bp) in 1.270 CPU sec, 1.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306250, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 231, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (226.56, 92.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (284.47, 144.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726772 reads in 174.748 CPU sec, 5.601 real sec
[M::mem_process_seqs] Processed 725850 reads in 99.941 CPU sec, 3.204 real sec
sorting block 29, #reads = 1103815 mem = 536879550 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305869, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 158, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (166.00, 63.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (285.68, 145.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 164, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480)
[M::mem_pestat] mean and std.dev: (190.14, 93.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 595)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724302 sequences (100000121 bp) in 2.608 CPU sec, 2.779 real sec...
[M::process] read 723414 sequences (100000120 bp) in 1.813 CPU sec, 1.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305633, 14, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 181, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (196.35, 61.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (289.54, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 51, 85)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197)
[M::mem_pestat] mean and std.dev: (51.08, 26.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 253)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 218, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (219.45, 102.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725396 reads in 174.055 CPU sec, 5.549 real sec
[M::mem_process_seqs] Processed 724752 reads in 100.925 CPU sec, 3.222 real sec
sorting block 30, #reads = 1102751 mem = 536876504 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305303, 8, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 164, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507)
[M::mem_pestat] mean and std.dev: (168.13, 63.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (290.42, 147.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 282, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2125)
[M::mem_pestat] mean and std.dev: (456.92, 308.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2778)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723186 sequences (100000245 bp) in 2.312 CPU sec, 2.479 real sec...
[M::process] read 723008 sequences (100000233 bp) in 1.397 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 304651, 9, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 353, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (317.73, 121.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 272, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (293.19, 147.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 147, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374)
[M::mem_pestat] mean and std.dev: (186.45, 74.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724302 reads in 175.551 CPU sec, 5.623 real sec
[M::mem_process_seqs] Processed 723414 reads in 100.876 CPU sec, 3.231 real sec
sorting block 31, #reads = 1101808 mem = 536880482 peak = 893501440
sorting block 32, #reads = 1101091 mem = 536877997 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 304255, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (295.13, 148.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 302, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (252.10, 62.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RR
[M::process] read 722696 sequences (100000251 bp) in 2.202 CPU sec, 2.339 real sec...
[M::process] read 727846 sequences (100000105 bp) in 1.359 CPU sec, 1.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 304323, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 231, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434)
[M::mem_pestat] mean and std.dev: (192.44, 52.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (295.93, 149.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 187, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (246.50, 204.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723186 reads in 172.066 CPU sec, 5.497 real sec
[M::mem_process_seqs] Processed 723008 reads in 98.968 CPU sec, 3.179 real sec
sorting block 33, #reads = 1100283 mem = 536877131 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 304121, 11, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 183, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (207.06, 64.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 277, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (298.32, 150.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (49, 66, 715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2047)
[M::mem_pestat] mean and std.dev: (277.91, 374.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2713)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 217, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (226.40, 98.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 727108 sequences (100000221 bp) in 1.931 CPU sec, 2.065 real sec...
[M::process] read 726692 sequences (100000053 bp) in 1.296 CPU sec, 1.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306419, 1, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 143, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348)
[M::mem_pestat] mean and std.dev: (155.88, 57.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.50, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 236, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (284.52, 99.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722696 reads in 171.280 CPU sec, 5.500 real sec
[M::mem_process_seqs] Processed 727846 reads in 97.324 CPU sec, 3.118 real sec
sorting block 34, #reads = 1100094 mem = 536878595 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306458, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 171, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (186.94, 55.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 521)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (280.83, 143.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 172, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (181.94, 58.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726396 sequences (100000130 bp) in 2.048 CPU sec, 2.155 real sec...
[M::process] read 726166 sequences (100000261 bp) in 1.250 CPU sec, 1.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306183, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 245, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (233.60, 72.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.89, 144.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 222, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449)
[M::mem_pestat] mean and std.dev: (214.90, 68.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 541)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727108 reads in 165.912 CPU sec, 5.315 real sec
[M::mem_process_seqs] Processed 726692 reads in 95.239 CPU sec, 3.061 real sec
sorting block 35, #reads = 1103520 mem = 536876851 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305752, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 199, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (217.43, 91.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.49, 144.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 222, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (232.21, 80.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 671)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 36, #reads = 1103854 mem = 536880945 peak = 893501440
[M::process] read 726162 sequences (100000041 bp) in 2.313 CPU sec, 2.518 real sec...
[M::process] read 726434 sequences (100000200 bp) in 1.510 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306175, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 183, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (217.42, 87.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.18, 144.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 153, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 297)
[M::mem_pestat] mean and std.dev: (144.45, 34.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726396 reads in 169.844 CPU sec, 5.431 real sec
[M::mem_process_seqs] Processed 726166 reads in 98.284 CPU sec, 3.138 real sec
sorting block 37, #reads = 1103026 mem = 536879353 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305981, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 149, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (173.53, 70.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.53, 144.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 196, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (198.53, 62.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728292 sequences (100000121 bp) in 2.127 CPU sec, 2.254 real sec...
[M::process] read 727832 sequences (100000160 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306176, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 218, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (200.76, 48.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.11, 143.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 183, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (224.17, 101.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726162 reads in 170.433 CPU sec, 5.447 real sec
[M::mem_process_seqs] Processed 726434 reads in 97.047 CPU sec, 3.113 real sec
sorting block 38, #reads = 1103221 mem = 536878120 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307170, 3, 22)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.88, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 198, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (181.05, 64.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 518)
[M::mem_pestat] skip orientation RR
[M::process] read 727530 sequences (100000089 bp) in 2.107 CPU sec, 2.255 real sec...
[M::process] read 727438 sequences (100000283 bp) in 1.447 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307329, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 169, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (183.07, 59.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (277.15, 142.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 197, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (218.40, 88.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728292 reads in 169.782 CPU sec, 5.428 real sec
[M::mem_process_seqs] Processed 727832 reads in 95.994 CPU sec, 3.071 real sec
sorting block 39, #reads = 1103908 mem = 536878847 peak = 893501440
sorting block 40, #reads = 1104677 mem = 536878976 peak = 893501440
[M::process] read 726998 sequences (100000136 bp) in 2.125 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306654, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 196, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 318)
[M::mem_pestat] mean and std.dev: (182.53, 41.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 373)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.32, 142.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 171, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (166.13, 61.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725778 sequences (100000076 bp) in 1.240 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 307083, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 215, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (208.48, 67.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (279.05, 143.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 174, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (167.13, 60.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 541)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727530 reads in 168.539 CPU sec, 5.389 real sec
[M::process] read 724358 sequences (100000155 bp) in 1.373 CPU sec, 1.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306493, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 185, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (195.48, 66.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.54, 143.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 161, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491)
[M::mem_pestat] mean and std.dev: (191.06, 87.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726998 reads in 94.630 CPU sec, 3.159 real sec
sorting block 41, #reads = 1104298 mem = 536880092 peak = 893501440
[M::mem_process_seqs] Processed 727438 reads in 221.881 CPU sec, 7.262 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306407, 3, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.72, 145.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 234, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (91, 361)
[M::mem_pestat] mean and std.dev: (223.94, 57.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 455)
[M::mem_pestat] skip orientation RR
[M::process] read 723570 sequences (100000024 bp) in 2.564 CPU sec, 2.866 real sec...
sorting block 42, #reads = 1104480 mem = 536879545 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305945, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 226, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (200.85, 71.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (288.80, 146.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 198, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (179.00, 75.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725778 reads in 180.257 CPU sec, 5.758 real sec
[M::process] read 722828 sequences (100000086 bp) in 1.273 CPU sec, 1.275 real sec...
[M::mem_process_seqs] Processed 724358 reads in 100.145 CPU sec, 3.194 real sec
sorting block 43, #reads = 1102978 mem = 536878050 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304949, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (233.95, 77.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (292.62, 147.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 196, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (108, 263)
[M::mem_pestat] mean and std.dev: (181.78, 23.21)
[M::mem_pestat] low and high boundaries for proper pairs: (77, 294)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 722524 sequences (100000173 bp) in 1.974 CPU sec, 2.106 real sec...
[M::process] read 728166 sequences (100000152 bp) in 1.318 CPU sec, 1.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 304399, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 252, 899)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2429)
[M::mem_pestat] mean and std.dev: (364.08, 280.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3194)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (294.86, 148.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 203, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (211.44, 91.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723570 reads in 169.476 CPU sec, 5.434 real sec
[M::mem_process_seqs] Processed 722828 reads in 96.445 CPU sec, 3.095 real sec
sorting block 44, #reads = 1101042 mem = 536878285 peak = 893501440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 303989, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 220, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 427)
[M::mem_pestat] mean and std.dev: (209.72, 53.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 275, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (296.89, 149.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 191, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 322)
[M::mem_pestat] mean and std.dev: (166.27, 40.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 381)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728430 sequences (100000040 bp) in 2.136 CPU sec, 2.285 real sec...
[M::process] read 727950 sequences (100000183 bp) in 1.559 CPU sec, 1.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306782, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 228, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (214.57, 92.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (280.72, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 160, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (165.19, 70.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 498)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722524 reads in 172.499 CPU sec, 5.514 real sec
[M::mem_process_seqs] Processed 728166 reads in 99.114 CPU sec, 3.166 real sec
sorting block 45, #reads = 1100612 mem = 536879390 peak = 893501440
[M::process] read 727342 sequences (100000081 bp) in 2.128 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306692, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 198, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (181.71, 45.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (279.10, 145.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 196, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (190.69, 78.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726808 sequences (100000170 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306630, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 195, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (177.35, 58.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 655)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (280.12, 145.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 129, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339)
[M::mem_pestat] mean and std.dev: (127.10, 29.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 411)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306371, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 214, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565)
[M::mem_pestat] mean and std.dev: (211.58, 58.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (282.02, 146.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 223, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (257.08, 145.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728430 reads in 251.788 CPU sec, 8.069 real sec
sorting block 46, #reads = 1103201 mem = 536879766 peak = 893501440
[M::mem_process_seqs] Processed 727950 reads in 176.435 CPU sec, 5.680 real sec
[M::mem_process_seqs] Processed 727342 reads in 185.162 CPU sec, 5.990 real sec
sorting block 47, #reads = 1104562 mem = 536881267 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306337, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 194, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (200.67, 54.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (282.39, 146.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 201, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (196.21, 74.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726808 reads in 97.422 CPU sec, 3.257 real sec
[M::process] read 726346 sequences (100000061 bp) in 3.414 CPU sec, 3.761 real sec...
sorting block 48, #reads = 1104191 mem = 536879251 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306046, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 174, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468)
[M::mem_pestat] mean and std.dev: (176.71, 74.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 583)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.74, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 191, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (190.62, 70.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726346 reads in 100.262 CPU sec, 3.309 real sec
[M::process] read 726456 sequences (100000225 bp) in 3.752 CPU sec, 4.444 real sec...
sorting block 49, #reads = 1103290 mem = 536879265 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305855, 0, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 237, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 398)
[M::mem_pestat] mean and std.dev: (223.33, 60.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (284.27, 146.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 184, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (232.79, 116.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725978 sequences (100000225 bp) in 2.812 CPU sec, 3.025 real sec...
[M::mem_process_seqs] Processed 726456 reads in 101.164 CPU sec, 3.298 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305881, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 203, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (184.87, 56.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.64, 145.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 160, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410)
[M::mem_pestat] mean and std.dev: (175.88, 80.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 501)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726268 sequences (100000148 bp) in 3.172 CPU sec, 3.460 real sec...
[M::mem_process_seqs] Processed 725978 reads in 103.675 CPU sec, 3.371 real sec
sorting block 50, #reads = 1103175 mem = 536878898 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306114, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 192, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561)
[M::mem_pestat] mean and std.dev: (346.82, 322.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2032)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.61, 145.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 201, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (240.12, 134.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726268 reads in 103.690 CPU sec, 3.387 real sec
[M::process] read 725706 sequences (100000084 bp) in 3.846 CPU sec, 4.231 real sec...
sorting block 51, #reads = 1102961 mem = 536878245 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305924, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 208, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (215.48, 55.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.31, 145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 199, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (236.35, 68.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725630 sequences (100000271 bp) in 3.027 CPU sec, 3.243 real sec...
[M::mem_process_seqs] Processed 725706 reads in 105.457 CPU sec, 3.419 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305457, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 143, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (212.00, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.70, 147.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 192, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (204.64, 84.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725222 sequences (100000173 bp) in 3.233 CPU sec, 3.493 real sec...
[M::mem_process_seqs] Processed 725630 reads in 103.897 CPU sec, 3.400 real sec
sorting block 52, #reads = 1103086 mem = 536879578 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305754, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 160, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (185.56, 60.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (289.18, 148.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (201.79, 70.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725072 sequences (100000172 bp) in 2.961 CPU sec, 3.160 real sec...
[M::mem_process_seqs] Processed 725222 reads in 103.968 CPU sec, 3.393 real sec
sorting block 53, #reads = 1102550 mem = 536880331 peak = 919568384
[M::process] read 724992 sequences (100000012 bp) in 2.960 CPU sec, 3.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304896, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 166, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (187.36, 69.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (289.35, 148.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 155, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355)
[M::mem_pestat] mean and std.dev: (169.09, 64.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725256 sequences (100000103 bp) in 1.879 CPU sec, 1.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304979, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 205, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (225.74, 94.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (289.58, 149.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 725072 reads in 191.276 CPU sec, 6.038 real sec
[M::mem_process_seqs] Processed 724992 reads in 114.826 CPU sec, 3.647 real sec
[M::process] read 725466 sequences (100000092 bp) in 2.115 CPU sec, 2.157 real sec...
sorting block 54, #reads = 1101854 mem = 536878069 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305219, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 204, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (225.00, 87.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 591)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (290.94, 151.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 194, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118)
[M::mem_pestat] mean and std.dev: (284.64, 178.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1437)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725322 sequences (100000192 bp) in 3.007 CPU sec, 3.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304738, 9, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 234, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 461)
[M::mem_pestat] mean and std.dev: (213.79, 62.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 265, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (292.07, 152.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 172, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346)
[M::mem_pestat] mean and std.dev: (172.53, 46.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725256 reads in 194.480 CPU sec, 6.242 real sec
sorting block 55, #reads = 1101918 mem = 536878232 peak = 919568384
[M::process] read 728458 sequences (100000190 bp) in 2.711 CPU sec, 2.886 real sec...
[M::process] read 728332 sequences (100000165 bp) in 1.529 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 305374, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 180, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (207.75, 105.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (292.63, 153.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 179, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (209.72, 81.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725322 reads in 105.755 CPU sec, 3.368 real sec
[M::mem_process_seqs] Processed 725466 reads in 281.470 CPU sec, 9.005 real sec
sorting block 56, #reads = 1102012 mem = 536879183 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307130, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 176, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (176.94, 54.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 483)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (280.35, 147.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 193, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (199.58, 55.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727644 sequences (100000255 bp) in 3.221 CPU sec, 3.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306939, 5, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 241, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (215.22, 99.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (278.62, 144.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 203, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (196.04, 72.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 541)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728458 reads in 214.888 CPU sec, 6.884 real sec
[M::mem_process_seqs] Processed 728332 reads in 122.309 CPU sec, 3.908 real sec
sorting block 57, #reads = 1102939 mem = 536880393 peak = 919568384
[M::process] read 727084 sequences (100000007 bp) in 2.807 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306311, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.17, 145.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 186, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 324)
[M::mem_pestat] mean and std.dev: (173.08, 36.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 387)
[M::mem_pestat] skip orientation RR
[M::process] read 726944 sequences (100000221 bp) in 2.325 CPU sec, 2.528 real sec...
[M::process] read 726668 sequences (100000158 bp) in 1.460 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306265, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 195, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (198.24, 75.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (281.49, 144.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 176, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 334)
[M::mem_pestat] mean and std.dev: (176.88, 39.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 391)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727644 reads in 201.248 CPU sec, 6.448 real sec
sorting block 58, #reads = 1104821 mem = 536880515 peak = 919568384
[M::mem_process_seqs] Processed 727084 reads in 119.125 CPU sec, 3.829 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306590, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 171, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (193.56, 65.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.52, 145.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 222, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (217.18, 86.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 59, #reads = 1103891 mem = 536878681 peak = 919568384
[M::process] read 726192 sequences (100000049 bp) in 3.302 CPU sec, 3.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306483, 6, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 171, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (204.18, 100.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.36, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 215, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (214.07, 97.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726944 reads in 192.043 CPU sec, 6.154 real sec
[M::mem_process_seqs] Processed 726668 reads in 115.573 CPU sec, 3.682 real sec
[M::process] read 726890 sequences (100000080 bp) in 3.831 CPU sec, 4.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305690, 0, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 146, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (170.00, 67.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.50, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 166, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (194.76, 72.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 60, #reads = 1104327 mem = 536879165 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306153, 9, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 219, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (189.46, 55.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 457)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (282.45, 145.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 727550 sequences (100000027 bp) in 3.493 CPU sec, 3.798 real sec...
[M::mem_process_seqs] Processed 726192 reads in 197.571 CPU sec, 6.364 real sec
[M::mem_process_seqs] Processed 726890 reads in 123.895 CPU sec, 3.998 real sec
sorting block 61, #reads = 1103381 mem = 536880702 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306880, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 179, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (205.61, 95.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.42, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 277, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (233.94, 84.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727550 reads in 106.351 CPU sec, 3.581 real sec
[M::process] read 726682 sequences (100000211 bp) in 4.078 CPU sec, 4.477 real sec...
sorting block 62, #reads = 1103425 mem = 536876195 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306705, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (245.06, 107.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.24, 145.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 182, 1659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4695)
[M::mem_pestat] mean and std.dev: (951.23, 1560.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7194)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725890 sequences (100000040 bp) in 3.131 CPU sec, 3.366 real sec...
[M::mem_process_seqs] Processed 726682 reads in 107.817 CPU sec, 3.598 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 306169, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 191, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (214.26, 100.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (285.89, 146.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 216, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (207.24, 70.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725636 sequences (100000005 bp) in 3.301 CPU sec, 3.555 real sec...
[M::mem_process_seqs] Processed 725890 reads in 106.372 CPU sec, 3.509 real sec
sorting block 63, #reads = 1104258 mem = 536879666 peak = 919568384
[M::process] read 725136 sequences (100000163 bp) in 2.736 CPU sec, 2.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305659, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 220, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (242.67, 95.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (287.76, 148.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 225, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (220.94, 83.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725460 sequences (100000227 bp) in 2.573 CPU sec, 2.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305726, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 197, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (205.67, 104.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (290.07, 149.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 182, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (185.50, 66.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725636 reads in 196.701 CPU sec, 6.268 real sec
sorting block 64, #reads = 1103107 mem = 536877660 peak = 919568384
[M::mem_process_seqs] Processed 725136 reads in 119.877 CPU sec, 3.823 real sec
[M::process] read 725204 sequences (100000225 bp) in 2.829 CPU sec, 2.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305552, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 345, 987)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595)
[M::mem_pestat] mean and std.dev: (525.36, 474.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3399)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (289.93, 149.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 223, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (247.29, 116.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 65, #reads = 1101860 mem = 536879925 peak = 919568384
[M::process] read 724998 sequences (100000002 bp) in 2.887 CPU sec, 3.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305503, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 154, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365)
[M::mem_pestat] mean and std.dev: (159.11, 50.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (291.05, 150.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482)
[M::mem_pestat] mean and std.dev: (211.05, 70.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725460 reads in 196.514 CPU sec, 6.295 real sec
[M::process] read 725076 sequences (100000258 bp) in 1.740 CPU sec, 1.756 real sec...
[M::mem_process_seqs] Processed 725204 reads in 120.513 CPU sec, 3.854 real sec
sorting block 66, #reads = 1102088 mem = 536877678 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305476, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 215, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (207.88, 68.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (292.85, 152.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 164, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (168.00, 52.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 465)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727868 sequences (100000067 bp) in 2.775 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305069, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 161, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (164.00, 71.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 265, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (292.73, 153.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 211, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (201.38, 83.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 635)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728042 sequences (100000121 bp) in 1.900 CPU sec, 1.900 real sec...
[M::mem_process_seqs] Processed 724998 reads in 192.942 CPU sec, 6.175 real sec
[M::mem_process_seqs] Processed 725076 reads in 120.744 CPU sec, 3.883 real sec
sorting block 67, #reads = 1102248 mem = 536879745 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306029, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 185, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410)
[M::mem_pestat] mean and std.dev: (194.81, 69.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (283.49, 149.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 218, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (267.08, 155.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727490 sequences (100000021 bp) in 3.414 CPU sec, 3.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306849, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 181, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (151.27, 69.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (279.26, 145.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 193, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 324)
[M::mem_pestat] mean and std.dev: (173.82, 47.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 379)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727868 reads in 194.176 CPU sec, 6.209 real sec
[M::mem_process_seqs] Processed 728042 reads in 121.486 CPU sec, 3.882 real sec
[M::process] read 726830 sequences (100000175 bp) in 2.300 CPU sec, 2.362 real sec...
sorting block 68, #reads = 1102185 mem = 536880314 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305947, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 185, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390)
[M::mem_pestat] mean and std.dev: (192.44, 65.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.44, 145.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 227, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (208.12, 87.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 69, #reads = 1104442 mem = 536877809 peak = 919568384
[M::process] read 726824 sequences (100000268 bp) in 3.262 CPU sec, 3.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306439, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 196, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (223.18, 88.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.62, 145.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (216.82, 74.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727490 reads in 199.028 CPU sec, 6.414 real sec
[M::mem_process_seqs] Processed 726830 reads in 125.029 CPU sec, 4.011 real sec
[M::process] read 726358 sequences (100000144 bp) in 2.488 CPU sec, 2.637 real sec...
sorting block 70, #reads = 1104141 mem = 536879005 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306006, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 266, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 561)
[M::mem_pestat] mean and std.dev: (270.94, 66.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (282.96, 145.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 193, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (221.07, 124.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 719)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726748 sequences (100000208 bp) in 3.283 CPU sec, 3.640 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306200, 1, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 318, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (228.69, 94.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.33, 145.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 219, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (245.33, 119.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726824 reads in 193.895 CPU sec, 6.227 real sec
[M::mem_process_seqs] Processed 726358 reads in 122.840 CPU sec, 3.944 real sec
[M::process] read 727716 sequences (100000158 bp) in 2.543 CPU sec, 2.731 real sec...
sorting block 71, #reads = 1103687 mem = 536878102 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306339, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 193, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 344)
[M::mem_pestat] mean and std.dev: (182.71, 64.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 440)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.02, 145.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 216, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413)
[M::mem_pestat] mean and std.dev: (198.29, 63.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 503)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728866 sequences (100000068 bp) in 2.799 CPU sec, 3.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306752, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 235, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (209.15, 72.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (279.64, 144.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 237, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (212.27, 84.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727868 sequences (100000274 bp) in 1.439 CPU sec, 1.435 real sec...
[M::mem_process_seqs] Processed 726748 reads in 197.957 CPU sec, 6.347 real sec
sorting block 72, #reads = 1103421 mem = 536876744 peak = 919568384
[M::mem_process_seqs] Processed 727716 reads in 121.670 CPU sec, 3.907 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307254, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (274.80, 142.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 213, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (215.00, 47.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RR
sorting block 73, #reads = 1104051 mem = 536878035 peak = 919568384
[M::process] read 726744 sequences (100000004 bp) in 3.135 CPU sec, 3.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306553, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 153, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438)
[M::mem_pestat] mean and std.dev: (160.96, 54.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 546)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (278.35, 143.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 212, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (224.31, 85.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726262 sequences (100000055 bp) in 1.829 CPU sec, 1.834 real sec...
[M::mem_process_seqs] Processed 728866 reads in 192.976 CPU sec, 6.171 real sec
[M::mem_process_seqs] Processed 727868 reads in 121.514 CPU sec, 3.903 real sec
sorting block 74, #reads = 1105407 mem = 536877963 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306539, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 146, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 278)
[M::mem_pestat] mean and std.dev: (155.23, 42.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 330)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.59, 145.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 135, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (199.73, 112.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725332 sequences (100000165 bp) in 3.224 CPU sec, 3.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306047, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 243, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (221.54, 88.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (285.52, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 207, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (255.58, 151.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726744 reads in 193.214 CPU sec, 6.193 real sec
[M::mem_process_seqs] Processed 726262 reads in 122.293 CPU sec, 3.907 real sec
[M::process] read 725028 sequences (100000151 bp) in 2.197 CPU sec, 2.275 real sec...
sorting block 75, #reads = 1102788 mem = 536878118 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305687, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 206, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 364)
[M::mem_pestat] mean and std.dev: (206.86, 51.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (288.79, 148.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 173, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 346)
[M::mem_pestat] mean and std.dev: (171.47, 51.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 410)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724784 sequences (100000163 bp) in 3.107 CPU sec, 3.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305377, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 215, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (218.64, 84.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (290.46, 149.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (203.20, 81.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725332 reads in 193.175 CPU sec, 6.187 real sec
[M::mem_process_seqs] Processed 725028 reads in 122.057 CPU sec, 3.901 real sec
[M::process] read 724702 sequences (100000127 bp) in 2.474 CPU sec, 2.526 real sec...
sorting block 76, #reads = 1103237 mem = 536880166 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305067, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 250, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (263.06, 130.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (293.18, 151.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 149, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (179.23, 73.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 77, #reads = 1101841 mem = 536879981 peak = 919568384
[M::process] read 725096 sequences (100000285 bp) in 3.151 CPU sec, 3.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304899, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 199, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (193.24, 77.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (293.09, 152.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 214, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (217.85, 106.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724784 reads in 183.878 CPU sec, 5.918 real sec
[M::mem_process_seqs] Processed 724702 reads in 119.751 CPU sec, 3.842 real sec
[M::process] read 727836 sequences (100000265 bp) in 2.312 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305363, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 215, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449)
[M::mem_pestat] mean and std.dev: (211.50, 68.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 549)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (293.29, 153.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 206, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (187.44, 81.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 78, #reads = 1102250 mem = 536879698 peak = 919568384
[M::process] read 727730 sequences (100000163 bp) in 3.224 CPU sec, 3.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 307214, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 296, 1851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5193)
[M::mem_pestat] mean and std.dev: (1285.30, 1785.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (283.74, 150.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 277, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (234.25, 91.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725096 reads in 173.042 CPU sec, 5.557 real sec
[M::mem_process_seqs] Processed 727836 reads in 113.431 CPU sec, 3.643 real sec
sorting block 79, #reads = 1101168 mem = 536879787 peak = 919568384
[M::process] read 727076 sequences (100000187 bp) in 3.083 CPU sec, 3.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306633, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 210, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (230.53, 91.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (281.22, 145.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 246, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (241.36, 65.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 684)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 306355, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 199, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426)
[M::mem_pestat] mean and std.dev: (198.54, 55.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (282.09, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 190, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (190.88, 56.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 527)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726914 sequences (100000219 bp) in 3.019 CPU sec, 3.290 real sec...
[M::mem_process_seqs] Processed 727730 reads in 178.349 CPU sec, 5.766 real sec
[M::mem_process_seqs] Processed 727076 reads in 117.035 CPU sec, 3.777 real sec
sorting block 80, #reads = 1104379 mem = 536878258 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306454, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 214, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (280.33, 228.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (282.88, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 151, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (217.91, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726470 sequences (100000124 bp) in 3.345 CPU sec, 3.708 real sec...
[M::mem_process_seqs] Processed 726914 reads in 99.408 CPU sec, 3.332 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306088, 2, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 172, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374)
[M::mem_pestat] mean and std.dev: (181.53, 50.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 452)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (283.71, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 198, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (198.69, 69.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 545)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 81, #reads = 1103777 mem = 536879170 peak = 919568384
[M::mem_process_seqs] Processed 726470 reads in 98.041 CPU sec, 3.296 real sec
[M::process] read 726504 sequences (100000166 bp) in 4.034 CPU sec, 4.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306086, 10, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 285, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (256.89, 84.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.69, 144.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 88, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200)
[M::mem_pestat] mean and std.dev: (71.80, 35.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 254)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 191, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438)
[M::mem_pestat] mean and std.dev: (187.19, 54.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 535)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726504 reads in 96.723 CPU sec, 3.221 real sec
sorting block 82, #reads = 1103601 mem = 536880150 peak = 919568384
[M::process] read 726796 sequences (100000275 bp) in 3.079 CPU sec, 3.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306155, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 156, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331)
[M::mem_pestat] mean and std.dev: (149.33, 37.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 399)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (280.61, 143.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 165, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 375)
[M::mem_pestat] mean and std.dev: (166.78, 49.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726796 reads in 101.089 CPU sec, 3.391 real sec
[M::process] read 728316 sequences (100000045 bp) in 3.697 CPU sec, 4.070 real sec...
sorting block 83, #reads = 1103215 mem = 536879655 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 307139, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 191, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (178.48, 65.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (276.59, 143.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 152, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (165.71, 59.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 456)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728316 reads in 96.350 CPU sec, 3.199 real sec
[M::process] read 728348 sequences (100000238 bp) in 3.018 CPU sec, 3.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306913, 0, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 187, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (201.96, 106.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (275.49, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 233, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (211.14, 79.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728348 reads in 93.531 CPU sec, 3.134 real sec
sorting block 84, #reads = 1103262 mem = 536878762 peak = 919568384
[M::process] read 728466 sequences (100000097 bp) in 3.830 CPU sec, 4.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307162, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 177, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (179.83, 58.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.45, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 155, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (189.00, 98.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728466 reads in 98.291 CPU sec, 3.258 real sec
sorting block 85, #reads = 1104737 mem = 536879816 peak = 919568384
[M::process] read 728048 sequences (100000033 bp) in 3.803 CPU sec, 4.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306672, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 190, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (183.47, 57.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.86, 142.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 182, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (192.20, 61.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727350 sequences (100000185 bp) in 2.857 CPU sec, 3.107 real sec...
[M::mem_process_seqs] Processed 728048 reads in 99.784 CPU sec, 3.248 real sec
sorting block 86, #reads = 1103609 mem = 536879886 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 307003, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 166, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 347)
[M::mem_pestat] mean and std.dev: (173.36, 62.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.15, 144.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 188, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (174.19, 52.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726306 sequences (100000092 bp) in 2.700 CPU sec, 2.908 real sec...
[M::mem_process_seqs] Processed 727350 reads in 98.313 CPU sec, 3.200 real sec
sorting block 87, #reads = 1103480 mem = 536878042 peak = 919568384
[M::process] read 725424 sequences (100000151 bp) in 2.364 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306162, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 234, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (194.64, 72.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.56, 144.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 238, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417)
[M::mem_pestat] mean and std.dev: (205.50, 61.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 503)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724580 sequences (100000051 bp) in 1.458 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305595, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 213, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (208.82, 115.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (288.52, 148.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 200, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393)
[M::mem_pestat] mean and std.dev: (181.08, 50.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724448 sequences (100000169 bp) in 1.449 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 726306 reads in 172.849 CPU sec, 5.475 real sec
[M::mem_process_seqs] Processed 725424 reads in 110.937 CPU sec, 3.563 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 304626, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 230, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (211.12, 108.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (291.92, 149.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 187, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (202.85, 84.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 647)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 88, #reads = 1102794 mem = 536878538 peak = 919568384
[M::process] read 724044 sequences (100000092 bp) in 3.502 CPU sec, 3.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 304764, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 189, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (228.33, 106.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 732)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 269, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (293.78, 151.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 231, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 395)
[M::mem_pestat] mean and std.dev: (214.00, 52.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 259506 sequences (35810083 bp) in 0.970 CPU sec, 0.966 real sec...
[M::mem_process_seqs] Processed 724580 reads in 177.210 CPU sec, 5.684 real sec
[M::mem_process_seqs] Processed 724448 reads in 116.217 CPU sec, 3.752 real sec
sorting block 89, #reads = 1101784 mem = 536879863 peak = 919568384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304942, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 186, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449)
[M::mem_pestat] mean and std.dev: (183.05, 49.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (294.67, 152.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 208, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (88, 328)
[M::mem_pestat] mean and std.dev: (201.65, 51.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 409)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 109293, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 268, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (295.09, 154.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 724044 reads in 120.844 CPU sec, 3.960 real sec
[M::mem_process_seqs] Processed 259506 reads in 51.360 CPU sec, 1.698 real sec
sorting block 90, #reads = 1101407 mem = 536878810 peak = 919568384
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 100459482
overall: 69532610560 mem 15031.249 user 1679.848 sys 577.975 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.SH032_HG514.L002\tPU:L002\tSM:SH032_HG514\tLB:SH032_HG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.SH032_HG514_R1.fastq.gz 0002.SH032_HG514_R2.fastq.gz
[main] Real time: 578.006 sec; CPU: 16711.101 sec
sorting block 91, #reads = 672445 mem = 327867189 peak = 919568384
uniq header count 1
merging 92 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 4441165846
output reads: 101041660
bam_mem_sort: 92 calls 54.957 user 3.369 sys 58.524 real
bam_write: 92 calls 31.540 user 1.830 sys 33.606 real
execute: 1 calls 27.621 user 1.863 sys 614.599 real
merge_files: 1 calls 27.603 user 1.817 sys 35.897 real
parse_chunk: 1466 calls 63.170 user 15.811 sys 79.913 real
read_chunk: 3969 calls 45.562 user 31.508 sys 479.095 real
sort_block: 1 calls 35.467 user 30.992 sys 462.598 real
write_chunk: 2501 calls 27.169 user 1.522 sys 34.931 real
overall: 2214871040 mem 1044.684 user 73.334 sys 614.617 real
ls: cannot access '*.cram': No such file or directory