File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/37/8a2c10bd45988aaddfeeb6df830164/.command.err
Size
218.1 KB
Task
DAQ:SENTIEON_BWAMEM (1_25PercentHG514;L002;0003)
Attempt

Content

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cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1_25PercentHG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1526.894 CPU sec, 53.314 real sec
[M::process] read 733132 sequences (100000282 bp) in 1.177 CPU sec, 1.184 real sec...
[M::process] read 731190 sequences (100000147 bp) in 1.831 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 310292, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (258.94, 131.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 181, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (196.50, 63.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] skip orientation RR
[M::process] read 730816 sequences (100000172 bp) in 1.803 CPU sec, 1.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309246, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (264.30, 133.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733132 reads in 174.670 CPU sec, 5.620 real sec
[M::process] read 730604 sequences (100000221 bp) in 1.939 CPU sec, 2.016 real sec...
[M::mem_process_seqs] Processed 731190 reads in 102.424 CPU sec, 3.355 real sec
sorting block 0, #reads = 1097478 mem = 536880343 peak = 608841728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308837, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.22, 134.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 177, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188)
[M::mem_pestat] mean and std.dev: (254.80, 176.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1541)
[M::mem_pestat] skip orientation RR
[M::process] read 730604 sequences (100000143 bp) in 2.491 CPU sec, 2.589 real sec...
[M::process] read 730104 sequences (100000102 bp) in 1.809 CPU sec, 1.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309044, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 206, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (192.78, 46.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (266.28, 134.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730816 reads in 172.502 CPU sec, 5.509 real sec
[M::mem_process_seqs] Processed 730604 reads in 101.533 CPU sec, 3.270 real sec
sorting block 1, #reads = 1095622 mem = 536877631 peak = 769273856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308822, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (266.81, 135.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 176, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (198.67, 82.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation RR
[M::process] read 729944 sequences (100000045 bp) in 2.128 CPU sec, 2.174 real sec...
[M::process] read 729504 sequences (100000102 bp) in 2.149 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308436, 9, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 138, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 320)
[M::mem_pestat] mean and std.dev: (159.18, 45.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 382)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.51, 135.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 358)
[M::mem_pestat] mean and std.dev: (191.78, 42.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730604 reads in 175.543 CPU sec, 5.608 real sec
[M::mem_process_seqs] Processed 730104 reads in 102.488 CPU sec, 3.303 real sec
sorting block 2, #reads = 1095491 mem = 536880545 peak = 789315584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308444, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (267.90, 134.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 3, #reads = 1095257 mem = 536879680 peak = 789315584
[M::process] read 729318 sequences (100000115 bp) in 2.557 CPU sec, 2.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308484, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 192, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (199.83, 95.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.50, 136.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729042 sequences (100000046 bp) in 1.940 CPU sec, 1.938 real sec...
[M::mem_process_seqs] Processed 729944 reads in 176.534 CPU sec, 5.647 real sec
[M::mem_process_seqs] Processed 729504 reads in 102.746 CPU sec, 3.305 real sec
sorting block 4, #reads = 1095210 mem = 536879404 peak = 789315584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308155, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (270.54, 136.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728566 sequences (100000011 bp) in 2.310 CPU sec, 2.376 real sec...
[M::process] read 728894 sequences (100000239 bp) in 1.999 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307509, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (270.08, 136.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 228, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (225.00, 60.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729318 reads in 180.205 CPU sec, 5.772 real sec
[M::mem_process_seqs] Processed 729042 reads in 107.231 CPU sec, 3.431 real sec
sorting block 5, #reads = 1094518 mem = 536878302 peak = 789315584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307710, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.46, 137.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 250, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 439)
[M::mem_pestat] mean and std.dev: (223.50, 63.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 526)
[M::mem_pestat] skip orientation RR
[M::process] read 729238 sequences (100000203 bp) in 2.656 CPU sec, 2.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307567, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.57, 137.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728878 sequences (100000048 bp) in 1.860 CPU sec, 1.857 real sec...
[M::mem_process_seqs] Processed 728566 reads in 179.326 CPU sec, 5.724 real sec
[M::mem_process_seqs] Processed 728894 reads in 104.013 CPU sec, 3.339 real sec
sorting block 6, #reads = 1094105 mem = 536878481 peak = 789315584
sorting block 7, #reads = 1093931 mem = 536878316 peak = 890277888
[M::process] read 728428 sequences (100000266 bp) in 1.740 CPU sec, 1.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308167, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.35, 137.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 727930 sequences (100000275 bp) in 1.530 CPU sec, 1.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307198, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (271.87, 136.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 347, 8700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25820)
[M::mem_pestat] mean and std.dev: (2768.60, 3883.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34380)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308370, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 267, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1585)
[M::mem_pestat] mean and std.dev: (408.18, 311.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2057)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.95, 137.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 187, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (171.33, 54.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729238 reads in 246.625 CPU sec, 7.947 real sec
[M::mem_process_seqs] Processed 728428 reads in 174.974 CPU sec, 5.665 real sec
[M::mem_process_seqs] Processed 728878 reads in 185.654 CPU sec, 6.045 real sec
sorting block 8, #reads = 1094686 mem = 536878186 peak = 890277888
[M::process] read 728034 sequences (100000226 bp) in 2.646 CPU sec, 2.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307282, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (273.86, 138.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 197, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (202.44, 73.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation RR
sorting block 9, #reads = 1094335 mem = 536878196 peak = 890277888
[M::process] read 727656 sequences (100000243 bp) in 1.641 CPU sec, 1.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307553, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 152, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (230.45, 152.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (274.03, 137.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 144, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393)
[M::mem_pestat] mean and std.dev: (181.45, 60.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727930 reads in 177.361 CPU sec, 5.687 real sec
[M::process] read 727424 sequences (100000072 bp) in 1.363 CPU sec, 1.378 real sec...
[M::mem_process_seqs] Processed 728034 reads in 98.220 CPU sec, 3.129 real sec
sorting block 10, #reads = 1093388 mem = 536878300 peak = 890277888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307375, 11, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 229, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (196.82, 70.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 253, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (274.85, 138.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 56, 126)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286)
[M::mem_pestat] mean and std.dev: (63.44, 28.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 366)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 727468 sequences (100000191 bp) in 3.183 CPU sec, 3.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307190, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 225, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (135, 335)
[M::mem_pestat] mean and std.dev: (224.40, 41.79)
[M::mem_pestat] low and high boundaries for proper pairs: (57, 392)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 253, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (275.21, 138.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727656 reads in 168.279 CPU sec, 5.355 real sec
[M::mem_process_seqs] Processed 727424 reads in 99.485 CPU sec, 3.154 real sec
[M::process] read 727030 sequences (100000078 bp) in 2.240 CPU sec, 2.287 real sec...
sorting block 11, #reads = 1093511 mem = 536877603 peak = 890277888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307359, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 223, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (199.15, 64.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (276.12, 139.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 212, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (207.70, 86.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 602)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727314 sequences (100000050 bp) in 2.127 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306817, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 155, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (201.62, 87.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (276.87, 139.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726962 sequences (100000231 bp) in 1.860 CPU sec, 1.867 real sec...
[M::mem_process_seqs] Processed 727468 reads in 169.979 CPU sec, 5.424 real sec
[M::mem_process_seqs] Processed 727030 reads in 100.702 CPU sec, 3.204 real sec
sorting block 12, #reads = 1093334 mem = 536880708 peak = 890277888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307041, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 159, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (200.82, 62.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (277.32, 139.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 13, #reads = 1092775 mem = 536876629 peak = 890277888
[M::process] read 726766 sequences (100000040 bp) in 2.240 CPU sec, 2.322 real sec...
[M::process] read 726350 sequences (100000256 bp) in 1.439 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306574, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 374, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (302.08, 115.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (278.79, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727314 reads in 168.504 CPU sec, 5.396 real sec
[M::mem_process_seqs] Processed 726962 reads in 100.654 CPU sec, 3.215 real sec
sorting block 14, #reads = 1092975 mem = 536877171 peak = 890277888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306247, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (278.92, 140.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 187, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (232.73, 124.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RR
[M::process] read 725978 sequences (100000284 bp) in 2.368 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306754, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (280.13, 140.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732760 sequences (100000125 bp) in 1.930 CPU sec, 1.928 real sec...
[M::mem_process_seqs] Processed 726766 reads in 170.962 CPU sec, 5.442 real sec
[M::mem_process_seqs] Processed 726350 reads in 100.531 CPU sec, 3.194 real sec
sorting block 15, #reads = 1092205 mem = 536877219 peak = 890277888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306564, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 200, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 408)
[M::mem_pestat] mean and std.dev: (198.17, 49.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 483)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (282.49, 141.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731840 sequences (100000261 bp) in 2.314 CPU sec, 2.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 310139, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (259.74, 131.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730902 sequences (100000164 bp) in 1.890 CPU sec, 1.890 real sec...
[M::mem_process_seqs] Processed 725978 reads in 168.052 CPU sec, 5.356 real sec
[M::mem_process_seqs] Processed 732760 reads in 99.208 CPU sec, 3.149 real sec
sorting block 16, #reads = 1092316 mem = 536877201 peak = 890277888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308962, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (262.26, 132.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 169, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 321)
[M::mem_pestat] mean and std.dev: (152.44, 33.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 377)
[M::mem_pestat] skip orientation RR
sorting block 17, #reads = 1095497 mem = 536879164 peak = 890277888
[M::process] read 730716 sequences (100000164 bp) in 2.660 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308778, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 159, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365)
[M::mem_pestat] mean and std.dev: (165.43, 49.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 442)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (265.28, 134.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 171, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207)
[M::mem_pestat] mean and std.dev: (391.30, 378.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1906)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731840 reads in 166.413 CPU sec, 5.311 real sec
[M::mem_process_seqs] Processed 730902 reads in 98.207 CPU sec, 3.107 real sec
[M::process] read 730632 sequences (100000250 bp) in 2.065 CPU sec, 2.087 real sec...
sorting block 18, #reads = 1096182 mem = 536878862 peak = 890277888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308970, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (265.85, 134.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 142, 168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 270)
[M::mem_pestat] mean and std.dev: (144.00, 33.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 321)
[M::mem_pestat] skip orientation RR
[M::process] read 730164 sequences (100000111 bp) in 2.374 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308885, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 176, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423)
[M::mem_pestat] mean and std.dev: (171.33, 45.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.35, 134.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 223, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (215.11, 69.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730154 sequences (100000261 bp) in 1.660 CPU sec, 1.658 real sec...
[M::mem_process_seqs] Processed 730716 reads in 164.779 CPU sec, 5.252 real sec
[M::mem_process_seqs] Processed 730632 reads in 96.986 CPU sec, 3.084 real sec
sorting block 19, #reads = 1096379 mem = 536879258 peak = 890277888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308857, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.44, 135.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730132 sequences (100000094 bp) in 2.911 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308855, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 224, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (206.12, 45.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (267.13, 134.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730164 reads in 166.297 CPU sec, 5.301 real sec
[M::mem_process_seqs] Processed 730154 reads in 98.334 CPU sec, 3.121 real sec
sorting block 20, #reads = 1095511 mem = 536879065 peak = 890277888
[M::process] read 729378 sequences (100000138 bp) in 2.371 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308989, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (268.14, 135.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1095758 mem = 536878213 peak = 894894080
[M::process] read 729388 sequences (100000141 bp) in 2.696 CPU sec, 2.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308328, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 197, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 316)
[M::mem_pestat] mean and std.dev: (192.17, 30.20)
[M::mem_pestat] low and high boundaries for proper pairs: (52, 360)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (269.64, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730132 reads in 170.638 CPU sec, 5.459 real sec
[M::mem_process_seqs] Processed 729378 reads in 101.282 CPU sec, 3.215 real sec
[M::process] read 729376 sequences (100000221 bp) in 2.335 CPU sec, 2.388 real sec...
sorting block 22, #reads = 1095506 mem = 536878570 peak = 894894080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307784, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.48, 136.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730206 sequences (100000087 bp) in 2.218 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307722, 13, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 279, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (260.93, 87.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.26, 137.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 59, 70)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 94)
[M::mem_pestat] mean and std.dev: (60.36, 7.19)
[M::mem_pestat] low and high boundaries for proper pairs: (22, 106)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 191, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 351)
[M::mem_pestat] mean and std.dev: (187.33, 43.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729388 reads in 172.237 CPU sec, 5.483 real sec
[M::process] read 730138 sequences (100000204 bp) in 1.990 CPU sec, 1.983 real sec...
[M::mem_process_seqs] Processed 729376 reads in 101.729 CPU sec, 3.227 real sec
sorting block 23, #reads = 1095084 mem = 536877091 peak = 906387456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308457, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 244, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (266.79, 134.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 44, 77)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217)
[M::mem_pestat] mean and std.dev: (35.11, 33.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 287)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 729376 sequences (100000101 bp) in 2.843 CPU sec, 2.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308616, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (265.84, 134.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730206 reads in 173.978 CPU sec, 5.558 real sec
[M::mem_process_seqs] Processed 730138 reads in 102.125 CPU sec, 3.231 real sec
sorting block 24, #reads = 1094593 mem = 536876442 peak = 906387456
[M::process] read 728934 sequences (100000239 bp) in 2.225 CPU sec, 2.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308486, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 158, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (168.30, 65.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (268.49, 135.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 187, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 345)
[M::mem_pestat] mean and std.dev: (163.90, 41.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 409)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 25, #reads = 1095415 mem = 536878238 peak = 912728064
[M::process] read 728490 sequences (100000190 bp) in 2.867 CPU sec, 3.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308013, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 201, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470)
[M::mem_pestat] mean and std.dev: (182.91, 61.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (270.50, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729376 reads in 164.885 CPU sec, 5.260 real sec
[M::mem_process_seqs] Processed 728934 reads in 98.701 CPU sec, 3.121 real sec
[M::process] read 728080 sequences (100000051 bp) in 2.973 CPU sec, 3.127 real sec...
sorting block 26, #reads = 1095131 mem = 536879891 peak = 912728064
[M::process] read 727858 sequences (100000002 bp) in 2.397 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308193, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 203, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (214.75, 88.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (273.86, 137.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728080 reads in 89.809 CPU sec, 2.833 real sec
sorting block 27, #reads = 1094216 mem = 536880006 peak = 912728064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307631, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 140, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (173.23, 67.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.73, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 727776 sequences (100000216 bp) in 2.562 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307189, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 236, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444)
[M::mem_pestat] mean and std.dev: (211.00, 60.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (274.24, 137.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 174, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319)
[M::mem_pestat] mean and std.dev: (155.46, 39.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 383)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728490 reads in 253.780 CPU sec, 8.096 real sec
[M::mem_process_seqs] Processed 727858 reads in 112.918 CPU sec, 3.595 real sec
[M::process] read 727280 sequences (100000091 bp) in 2.593 CPU sec, 2.659 real sec...
sorting block 28, #reads = 1094035 mem = 536878030 peak = 912728064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 307001, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (275.58, 138.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 726774 sequences (100000042 bp) in 3.248 CPU sec, 3.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306742, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 269, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (225.46, 88.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (276.36, 138.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727776 reads in 167.154 CPU sec, 5.339 real sec
[M::mem_process_seqs] Processed 727280 reads in 100.955 CPU sec, 3.215 real sec
sorting block 29, #reads = 1093639 mem = 536876939 peak = 912728064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 306815, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 726908 sequences (100000164 bp) in 3.166 CPU sec, 3.386 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (168, 256, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (277.07, 138.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306662, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (277.77, 139.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 179, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 367)
[M::mem_pestat] mean and std.dev: (178.90, 60.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 436)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726774 reads in 168.891 CPU sec, 5.458 real sec
[M::mem_process_seqs] Processed 726908 reads in 100.643 CPU sec, 3.220 real sec
[M::process] read 726514 sequences (100000071 bp) in 3.129 CPU sec, 3.290 real sec...
sorting block 30, #reads = 1092610 mem = 536880846 peak = 912728064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306776, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 173, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 325)
[M::mem_pestat] mean and std.dev: (164.30, 35.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 381)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (279.59, 140.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726514 reads in 92.273 CPU sec, 3.084 real sec
[M::process] read 726296 sequences (100000169 bp) in 3.839 CPU sec, 4.206 real sec...
sorting block 31, #reads = 1092505 mem = 536879120 peak = 912728064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306441, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (278.65, 139.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726296 reads in 91.110 CPU sec, 3.004 real sec
[M::process] read 726200 sequences (100000024 bp) in 3.474 CPU sec, 3.725 real sec...
sorting block 32, #reads = 1092544 mem = 536878387 peak = 912728064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306911, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 182, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474)
[M::mem_pestat] mean and std.dev: (184.15, 58.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 583)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (280.52, 140.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726200 reads in 90.851 CPU sec, 2.947 real sec
[M::process] read 725916 sequences (100000193 bp) in 2.928 CPU sec, 3.027 real sec...
sorting block 33, #reads = 1092259 mem = 536879441 peak = 912728064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305902, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 180, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (183.62, 59.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (282.64, 141.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732012 sequences (100000092 bp) in 2.667 CPU sec, 2.771 real sec...
[M::mem_process_seqs] Processed 725916 reads in 92.571 CPU sec, 2.993 real sec
[M::process] read 731394 sequences (100000245 bp) in 2.357 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308773, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (262.09, 132.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 190, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (202.80, 85.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RR
[M::process] read 730982 sequences (100000094 bp) in 1.940 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308891, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 143, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (186.73, 74.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 703)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (264.00, 133.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732012 reads in 167.649 CPU sec, 5.279 real sec
[M::mem_process_seqs] Processed 731394 reads in 99.181 CPU sec, 3.136 real sec
sorting block 34, #reads = 1091751 mem = 536878162 peak = 912728064
[M::process] read 730676 sequences (100000037 bp) in 2.336 CPU sec, 2.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308586, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.19, 134.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (212.50, 104.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RR
sorting block 35, #reads = 1097017 mem = 536880087 peak = 959135744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308967, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.09, 134.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730446 sequences (100000018 bp) in 3.311 CPU sec, 3.605 real sec...
[M::mem_process_seqs] Processed 730982 reads in 163.893 CPU sec, 5.259 real sec
[M::mem_process_seqs] Processed 730676 reads in 99.262 CPU sec, 3.165 real sec
sorting block 36, #reads = 1095767 mem = 536880825 peak = 959135744
[M::process] read 730612 sequences (100000187 bp) in 2.816 CPU sec, 2.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309110, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.50, 135.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730118 sequences (100000064 bp) in 2.433 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309138, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 317, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 561)
[M::mem_pestat] mean and std.dev: (282.33, 68.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.49, 136.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 206, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (201.13, 57.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730446 reads in 163.848 CPU sec, 5.221 real sec
[M::mem_process_seqs] Processed 730612 reads in 97.377 CPU sec, 3.093 real sec
sorting block 37, #reads = 1096312 mem = 536879770 peak = 959135744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308293, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 170, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (186.69, 81.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (268.04, 135.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (246.59, 92.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730118 reads in 87.629 CPU sec, 2.937 real sec
[M::process] read 729830 sequences (100000131 bp) in 3.500 CPU sec, 3.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308398, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.31, 136.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 185, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (240.18, 132.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729830 reads in 86.784 CPU sec, 2.888 real sec
sorting block 38, #reads = 1095720 mem = 536878457 peak = 959135744
[M::process] read 729510 sequences (100000139 bp) in 3.169 CPU sec, 3.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307976, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 175, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 289)
[M::mem_pestat] mean and std.dev: (157.56, 28.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 339)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (270.10, 136.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 187, 190)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 248)
[M::mem_pestat] mean and std.dev: (181.78, 18.75)
[M::mem_pestat] low and high boundaries for proper pairs: (74, 277)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 39, #reads = 1094971 mem = 536879994 peak = 959135744
[M::mem_process_seqs] Processed 729510 reads in 90.762 CPU sec, 2.982 real sec
[M::process] read 729202 sequences (100000276 bp) in 3.091 CPU sec, 3.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308312, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 249, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.50, 136.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729916 sequences (100000042 bp) in 2.711 CPU sec, 2.824 real sec...
[M::mem_process_seqs] Processed 729202 reads in 87.854 CPU sec, 2.849 real sec
sorting block 40, #reads = 1094877 mem = 536879082 peak = 959135744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308138, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.85, 136.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 160, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348)
[M::mem_pestat] mean and std.dev: (163.85, 58.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729916 reads in 87.580 CPU sec, 2.838 real sec
[M::process] read 730768 sequences (100000116 bp) in 3.189 CPU sec, 3.415 real sec...
sorting block 41, #reads = 1094581 mem = 536877235 peak = 959135744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308657, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (265.49, 134.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730768 reads in 89.591 CPU sec, 2.909 real sec
[M::process] read 731030 sequences (100000102 bp) in 3.095 CPU sec, 3.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309173, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 145, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 307)
[M::mem_pestat] mean and std.dev: (154.60, 37.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 368)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (264.18, 133.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731030 reads in 88.022 CPU sec, 2.867 real sec
sorting block 42, #reads = 1096064 mem = 536880636 peak = 959135744
[M::process] read 730384 sequences (100000105 bp) in 3.613 CPU sec, 3.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308650, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 173, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (215.00, 101.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (265.66, 134.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 167, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (182.50, 85.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729214 sequences (100000253 bp) in 2.547 CPU sec, 2.698 real sec...
[M::mem_process_seqs] Processed 730384 reads in 91.060 CPU sec, 2.952 real sec
sorting block 43, #reads = 1096088 mem = 536879507 peak = 959135744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307998, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (269.20, 135.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729214 reads in 90.328 CPU sec, 2.925 real sec
[M::process] read 729018 sequences (100000115 bp) in 3.328 CPU sec, 3.578 real sec...
[M::process] read 728726 sequences (100000124 bp) in 2.219 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307945, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 195, 633)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543)
[M::mem_pestat] mean and std.dev: (345.55, 302.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (270.86, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 184, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348)
[M::mem_pestat] mean and std.dev: (185.40, 82.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 515)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728052 sequences (100000174 bp) in 1.570 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307898, 9, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 211, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565)
[M::mem_pestat] mean and std.dev: (183.50, 65.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (270.87, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729018 reads in 164.895 CPU sec, 5.187 real sec
[M::process] read 727616 sequences (100000095 bp) in 1.576 CPU sec, 1.592 real sec...
[M::mem_process_seqs] Processed 728726 reads in 97.469 CPU sec, 3.072 real sec
sorting block 44, #reads = 1094628 mem = 536881949 peak = 959135744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307645, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (272.84, 136.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 143, 173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 257)
[M::mem_pestat] mean and std.dev: (149.89, 27.67)
[M::mem_pestat] low and high boundaries for proper pairs: (5, 299)
[M::mem_pestat] skip orientation RR
sorting block 45, #reads = 1094771 mem = 536881476 peak = 989868032
[M::process] read 727396 sequences (100000245 bp) in 3.264 CPU sec, 3.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307440, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 197, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (203.10, 93.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (276.04, 138.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728052 reads in 160.769 CPU sec, 5.154 real sec
[M::mem_process_seqs] Processed 727616 reads in 97.627 CPU sec, 3.102 real sec
sorting block 46, #reads = 1093920 mem = 536878573 peak = 989868032
[M::process] read 726964 sequences (100000224 bp) in 2.521 CPU sec, 2.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307277, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (276.36, 139.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 726302 sequences (100000053 bp) in 2.221 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307006, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 185, 876)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336)
[M::mem_pestat] mean and std.dev: (377.60, 328.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3066)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (277.27, 138.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727396 reads in 160.146 CPU sec, 5.082 real sec
[M::mem_process_seqs] Processed 726964 reads in 96.164 CPU sec, 3.048 real sec
[M::process] read 726400 sequences (100000161 bp) in 2.790 CPU sec, 2.923 real sec...
sorting block 47, #reads = 1093517 mem = 536878973 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306404, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (280.16, 139.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 205, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 414)
[M::mem_pestat] mean and std.dev: (198.25, 54.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 306434, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (280.30, 140.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726302 reads in 168.246 CPU sec, 5.409 real sec
[M::process] read 725806 sequences (100000194 bp) in 3.152 CPU sec, 3.408 real sec...
[M::mem_process_seqs] Processed 726400 reads in 101.734 CPU sec, 3.241 real sec
sorting block 48, #reads = 1092254 mem = 536879858 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306745, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 230, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249)
[M::mem_pestat] mean and std.dev: (345.82, 294.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1622)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 260, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (280.86, 139.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 314)
[M::mem_pestat] mean and std.dev: (171.50, 45.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 371)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725806 reads in 90.760 CPU sec, 3.000 real sec
[M::process] read 725948 sequences (100000007 bp) in 3.371 CPU sec, 3.719 real sec...
sorting block 49, #reads = 1092556 mem = 536877632 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 305864, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (281.88, 140.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 222, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (194.50, 78.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725948 reads in 91.943 CPU sec, 3.024 real sec
[M::process] read 725486 sequences (100000090 bp) in 2.933 CPU sec, 3.216 real sec...
sorting block 50, #reads = 1092072 mem = 536881743 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305910, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 174, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423)
[M::mem_pestat] mean and std.dev: (167.25, 39.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (283.56, 141.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 725486 reads in 88.694 CPU sec, 2.905 real sec
[M::process] read 731426 sequences (100000167 bp) in 3.401 CPU sec, 3.736 real sec...
sorting block 51, #reads = 1091146 mem = 536877667 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309337, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.14, 134.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 180, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (168.70, 52.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731426 reads in 88.736 CPU sec, 2.908 real sec
[M::process] read 731296 sequences (100000189 bp) in 3.148 CPU sec, 3.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308871, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (264.30, 133.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731022 sequences (100000180 bp) in 2.542 CPU sec, 2.634 real sec...
[M::mem_process_seqs] Processed 731296 reads in 85.992 CPU sec, 2.786 real sec
sorting block 52, #reads = 1095228 mem = 536876947 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308101, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (265.83, 134.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 167, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (159.18, 44.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation RR
[M::process] read 730524 sequences (100000034 bp) in 2.684 CPU sec, 2.783 real sec...
[M::mem_process_seqs] Processed 731022 reads in 88.340 CPU sec, 2.844 real sec
sorting block 53, #reads = 1095966 mem = 536880068 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308637, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.15, 135.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730350 sequences (100000056 bp) in 2.656 CPU sec, 2.761 real sec...
[M::mem_process_seqs] Processed 730524 reads in 88.393 CPU sec, 2.860 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308141, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 729940 sequences (100000249 bp) in 2.646 CPU sec, 2.743 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.86, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730314 sequences (100000252 bp) in 1.950 CPU sec, 1.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308533, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.59, 135.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 187, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (192.83, 56.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 531)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730350 reads in 160.557 CPU sec, 5.099 real sec
sorting block 54, #reads = 1095687 mem = 536878497 peak = 989868032
[M::mem_process_seqs] Processed 729940 reads in 96.357 CPU sec, 3.058 real sec
[M::process] read 730152 sequences (100000038 bp) in 2.353 CPU sec, 2.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308727, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.57, 135.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1095363 mem = 536879531 peak = 989868032
[M::process] read 729988 sequences (100000155 bp) in 3.130 CPU sec, 3.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308735, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 164, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (213.27, 118.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (267.74, 135.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730314 reads in 162.322 CPU sec, 5.195 real sec
[M::mem_process_seqs] Processed 730152 reads in 99.160 CPU sec, 3.159 real sec
sorting block 56, #reads = 1095463 mem = 536880592 peak = 989868032
[M::process] read 730416 sequences (100000256 bp) in 2.830 CPU sec, 3.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308636, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.67, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308774, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (266.92, 134.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 215, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (209.27, 85.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation RR
[M::process] read 730868 sequences (100000086 bp) in 2.633 CPU sec, 2.845 real sec...
[M::mem_process_seqs] Processed 729988 reads in 162.775 CPU sec, 5.183 real sec
[M::mem_process_seqs] Processed 730416 reads in 97.008 CPU sec, 3.080 real sec
sorting block 57, #reads = 1095474 mem = 536879367 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308998, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (264.94, 134.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730868 reads in 89.555 CPU sec, 2.998 real sec
[M::process] read 731648 sequences (100000083 bp) in 3.598 CPU sec, 3.906 real sec...
sorting block 58, #reads = 1095490 mem = 536877439 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309812, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 193, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (232.67, 114.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 239, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (262.40, 133.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 181, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (166.89, 36.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 503)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731648 reads in 87.843 CPU sec, 2.889 real sec
[M::process] read 731028 sequences (100000284 bp) in 3.415 CPU sec, 3.740 real sec...
sorting block 59, #reads = 1096681 mem = 536876107 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308951, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (264.81, 134.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731416 sequences (100000267 bp) in 2.673 CPU sec, 2.804 real sec...
[M::mem_process_seqs] Processed 731028 reads in 88.774 CPU sec, 2.876 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 309014, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.92, 134.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731132 sequences (100000132 bp) in 2.649 CPU sec, 2.749 real sec...
[M::mem_process_seqs] Processed 731416 reads in 87.801 CPU sec, 2.845 real sec
sorting block 60, #reads = 1096200 mem = 536879591 peak = 989868032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309197, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (264.02, 133.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731132 reads in 89.193 CPU sec, 2.891 real sec
[M::process] read 730922 sequences (100000111 bp) in 3.175 CPU sec, 3.343 real sec...
sorting block 61, #reads = 1096461 mem = 536880271 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308809, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.35, 135.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 198, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (104, 289)
[M::mem_pestat] mean and std.dev: (202.20, 31.75)
[M::mem_pestat] low and high boundaries for proper pairs: (67, 329)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730922 reads in 86.129 CPU sec, 2.809 real sec
[M::process] read 730724 sequences (100000049 bp) in 3.381 CPU sec, 3.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309321, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 139, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517)
[M::mem_pestat] mean and std.dev: (149.30, 61.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (265.42, 134.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729952 sequences (100000242 bp) in 2.466 CPU sec, 2.704 real sec...
[M::mem_process_seqs] Processed 730724 reads in 87.439 CPU sec, 2.835 real sec
sorting block 62, #reads = 1096044 mem = 536879348 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308064, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.59, 135.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729952 reads in 87.804 CPU sec, 2.847 real sec
[M::process] read 729986 sequences (100000086 bp) in 3.640 CPU sec, 3.936 real sec...
sorting block 63, #reads = 1095587 mem = 536877272 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308678, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 198, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (198.77, 112.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.06, 137.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (97, 188, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (215.45, 123.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729986 reads in 87.097 CPU sec, 2.823 real sec
[M::process] read 728650 sequences (100000134 bp) in 2.981 CPU sec, 3.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307907, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (271.71, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 153, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 288)
[M::mem_pestat] mean and std.dev: (146.40, 27.64)
[M::mem_pestat] low and high boundaries for proper pairs: (18, 333)
[M::mem_pestat] skip orientation RR
[M::process] read 727692 sequences (100000264 bp) in 2.577 CPU sec, 2.659 real sec...
[M::mem_process_seqs] Processed 728650 reads in 86.868 CPU sec, 2.817 real sec
sorting block 64, #reads = 1095125 mem = 536881067 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307469, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 259, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (256.28, 87.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 253, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (274.55, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726834 sequences (100000097 bp) in 2.719 CPU sec, 2.825 real sec...
[M::mem_process_seqs] Processed 727692 reads in 88.322 CPU sec, 2.861 real sec
sorting block 65, #reads = 1094157 mem = 536881647 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306910, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 177, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (209.36, 76.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (277.35, 138.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 177, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344)
[M::mem_pestat] mean and std.dev: (173.00, 51.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 414)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726834 reads in 87.870 CPU sec, 2.855 real sec
[M::process] read 726432 sequences (100000176 bp) in 3.396 CPU sec, 3.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 306278, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (279.22, 139.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 151, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (170.17, 57.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726432 reads in 89.532 CPU sec, 2.919 real sec
sorting block 66, #reads = 1092724 mem = 536876470 peak = 1005924352
[M::process] read 726402 sequences (100000267 bp) in 3.406 CPU sec, 3.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306508, 2, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 159, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 286)
[M::mem_pestat] mean and std.dev: (165.91, 41.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (279.73, 139.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 157, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408)
[M::mem_pestat] mean and std.dev: (159.35, 41.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725750 sequences (100000161 bp) in 2.634 CPU sec, 2.797 real sec...
[M::mem_process_seqs] Processed 726402 reads in 87.299 CPU sec, 2.835 real sec
sorting block 67, #reads = 1092407 mem = 536878252 peak = 1005924352
[M::process] read 725204 sequences (100000085 bp) in 2.338 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306192, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (281.63, 140.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733976 sequences (100000218 bp) in 1.710 CPU sec, 1.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305759, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 203, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (206.64, 80.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (282.93, 140.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 725750 reads in 161.539 CPU sec, 5.092 real sec
[M::process] read 732440 sequences (100000179 bp) in 2.844 CPU sec, 2.972 real sec...
sorting block 68, #reads = 1091532 mem = 536879386 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 310524, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 197, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417)
[M::mem_pestat] mean and std.dev: (188.70, 46.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 501)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 235, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (260.81, 135.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 141, 171)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 283)
[M::mem_pestat] mean and std.dev: (138.67, 41.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 339)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 733976 reads in 92.172 CPU sec, 2.936 real sec
[M::mem_process_seqs] Processed 725204 reads in 227.441 CPU sec, 7.203 real sec
[M::process] read 732102 sequences (100000117 bp) in 2.058 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309352, 9, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 171, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465)
[M::mem_pestat] mean and std.dev: (169.64, 49.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 574)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (264.21, 136.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 69, #reads = 1096178 mem = 536878761 peak = 1005924352
[M::process] read 731402 sequences (100000009 bp) in 2.760 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309529, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (264.66, 136.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732440 reads in 178.202 CPU sec, 5.641 real sec
[M::mem_process_seqs] Processed 732102 reads in 100.441 CPU sec, 3.188 real sec
[M::process] read 731180 sequences (100000261 bp) in 2.868 CPU sec, 3.038 real sec...
sorting block 70, #reads = 1094793 mem = 536879669 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308782, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (266.24, 136.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308400, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (266.97, 137.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730640 sequences (100000101 bp) in 3.140 CPU sec, 3.391 real sec...
[M::mem_process_seqs] Processed 731402 reads in 171.224 CPU sec, 5.498 real sec
[M::mem_process_seqs] Processed 731180 reads in 102.373 CPU sec, 3.262 real sec
sorting block 71, #reads = 1097302 mem = 536878476 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308397, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.21, 136.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 197, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 308)
[M::mem_pestat] mean and std.dev: (188.00, 24.18)
[M::mem_pestat] low and high boundaries for proper pairs: (20, 356)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730640 reads in 91.358 CPU sec, 3.049 real sec
[M::process] read 730840 sequences (100000066 bp) in 3.424 CPU sec, 3.736 real sec...
sorting block 72, #reads = 1096291 mem = 536877251 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309106, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 306, 918)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2450)
[M::mem_pestat] mean and std.dev: (488.00, 354.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.41, 137.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 180, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (230.70, 95.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730840 reads in 89.448 CPU sec, 2.977 real sec
[M::process] read 730192 sequences (100000000 bp) in 3.161 CPU sec, 3.403 real sec...
sorting block 73, #reads = 1095814 mem = 536880795 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308310, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (268.94, 136.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (106, 154, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (190.00, 114.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730192 reads in 88.996 CPU sec, 2.913 real sec
[M::process] read 729804 sequences (100000221 bp) in 3.092 CPU sec, 3.323 real sec...
sorting block 74, #reads = 1095746 mem = 536879029 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308015, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 207, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (186.17, 71.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 531)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.04, 136.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729804 reads in 89.356 CPU sec, 2.888 real sec
[M::process] read 729722 sequences (100000256 bp) in 2.804 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308261, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (269.95, 137.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 205, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411)
[M::mem_pestat] mean and std.dev: (212.23, 64.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729722 reads in 88.977 CPU sec, 2.883 real sec
[M::process] read 729322 sequences (100000104 bp) in 3.595 CPU sec, 3.856 real sec...
sorting block 75, #reads = 1095187 mem = 536878965 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307487, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (270.89, 137.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729322 reads in 91.004 CPU sec, 2.957 real sec
[M::process] read 729588 sequences (100000000 bp) in 3.318 CPU sec, 3.522 real sec...
sorting block 76, #reads = 1094201 mem = 536879285 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307865, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.29, 137.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729588 reads in 91.660 CPU sec, 2.963 real sec
[M::process] read 729722 sequences (100000040 bp) in 2.901 CPU sec, 3.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307777, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.46, 137.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729722 reads in 91.625 CPU sec, 2.986 real sec
sorting block 77, #reads = 1094560 mem = 536879164 peak = 1005924352
[M::process] read 729186 sequences (100000219 bp) in 4.032 CPU sec, 4.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308017, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 168, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (200.64, 93.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (270.82, 137.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 191, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (176.00, 38.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 497)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729186 reads in 91.528 CPU sec, 2.965 real sec
[M::process] read 728778 sequences (100000011 bp) in 2.974 CPU sec, 3.157 real sec...
sorting block 78, #reads = 1094523 mem = 536879266 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308005, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 247, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (269.68, 136.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728472 sequences (100000139 bp) in 2.759 CPU sec, 2.906 real sec...
[M::mem_process_seqs] Processed 728778 reads in 90.274 CPU sec, 2.932 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307358, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 279, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (254.10, 138.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.60, 138.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728472 reads in 91.701 CPU sec, 2.985 real sec
[M::process] read 728280 sequences (100000144 bp) in 3.453 CPU sec, 3.727 real sec...
sorting block 79, #reads = 1094102 mem = 536880373 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307331, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.75, 139.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 176, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (217.54, 86.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RR
[M::process] read 728436 sequences (100000145 bp) in 2.811 CPU sec, 2.950 real sec...
[M::mem_process_seqs] Processed 728280 reads in 91.972 CPU sec, 2.977 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307374, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 194, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470)
[M::mem_pestat] mean and std.dev: (187.22, 50.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (274.88, 140.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 80, #reads = 1093610 mem = 536880205 peak = 1005924352
[M::mem_process_seqs] Processed 728436 reads in 89.519 CPU sec, 2.917 real sec
[M::process] read 728466 sequences (100000231 bp) in 3.354 CPU sec, 3.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307176, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 172, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361)
[M::mem_pestat] mean and std.dev: (304.73, 274.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1769)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.66, 139.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728466 reads in 91.408 CPU sec, 2.968 real sec
[M::process] read 728424 sequences (100000250 bp) in 3.077 CPU sec, 3.304 real sec...
sorting block 81, #reads = 1093241 mem = 536880663 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307894, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.34, 139.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 252, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (207.62, 50.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728424 reads in 90.581 CPU sec, 2.940 real sec
[M::process] read 728432 sequences (100000162 bp) in 3.643 CPU sec, 3.904 real sec...
sorting block 82, #reads = 1093942 mem = 536878452 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307062, 8, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 160, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (161.93, 59.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (276.60, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 177, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (166.83, 52.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 655)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728502 sequences (100000206 bp) in 2.668 CPU sec, 2.846 real sec...
[M::mem_process_seqs] Processed 728432 reads in 89.909 CPU sec, 2.898 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 307331, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (277.17, 142.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 194, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407)
[M::mem_pestat] mean and std.dev: (169.75, 37.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728502 reads in 90.284 CPU sec, 2.926 real sec
sorting block 83, #reads = 1093471 mem = 536877497 peak = 1005924352
[M::process] read 728364 sequences (100000199 bp) in 3.614 CPU sec, 3.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306836, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (276.94, 142.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 216, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750)
[M::mem_pestat] mean and std.dev: (349.09, 260.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2268)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728364 reads in 90.410 CPU sec, 2.931 real sec
[M::process] read 728516 sequences (100000052 bp) in 2.751 CPU sec, 2.966 real sec...
sorting block 84, #reads = 1093503 mem = 536880280 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307320, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 163, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (218.62, 104.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (277.20, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728516 reads in 89.148 CPU sec, 2.889 real sec
[M::process] read 728712 sequences (100000197 bp) in 3.806 CPU sec, 4.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307551, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (276.95, 144.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 232, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (222.30, 80.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728712 reads in 89.619 CPU sec, 2.897 real sec
[M::process] read 733152 sequences (100000205 bp) in 2.815 CPU sec, 2.994 real sec...
sorting block 85, #reads = 1093962 mem = 536877134 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309247, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (263.23, 137.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733152 reads in 89.483 CPU sec, 2.913 real sec
[M::process] read 732930 sequences (100000078 bp) in 3.570 CPU sec, 3.773 real sec...
sorting block 86, #reads = 1095655 mem = 536879887 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309751, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 148, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 272)
[M::mem_pestat] mean and std.dev: (153.00, 44.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (262.75, 135.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 159, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (181.90, 85.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 731918 sequences (100000153 bp) in 2.560 CPU sec, 2.807 real sec...
[M::mem_process_seqs] Processed 732930 reads in 90.533 CPU sec, 2.927 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309541, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 241, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (264.65, 135.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731918 reads in 90.226 CPU sec, 2.936 real sec
[M::process] read 731388 sequences (100000054 bp) in 2.962 CPU sec, 3.098 real sec...
sorting block 87, #reads = 1097830 mem = 536879049 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309108, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 146, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 329)
[M::mem_pestat] mean and std.dev: (156.40, 26.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (266.50, 136.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 233, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431)
[M::mem_pestat] mean and std.dev: (200.31, 55.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731388 reads in 89.202 CPU sec, 2.901 real sec
[M::process] read 730908 sequences (100000100 bp) in 3.114 CPU sec, 3.392 real sec...
sorting block 88, #reads = 1096421 mem = 536879696 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308306, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 217, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (199.78, 61.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.69, 136.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 182, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (198.00, 78.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730908 reads in 89.492 CPU sec, 2.912 real sec
[M::process] read 730786 sequences (100000010 bp) in 3.045 CPU sec, 3.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308653, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.98, 135.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730786 reads in 89.718 CPU sec, 2.916 real sec
[M::process] read 730736 sequences (100000191 bp) in 3.054 CPU sec, 3.299 real sec...
sorting block 89, #reads = 1095837 mem = 536877930 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308899, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.71, 137.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 140, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (182.50, 81.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 628)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730736 reads in 88.277 CPU sec, 2.876 real sec
[M::process] read 730050 sequences (100000241 bp) in 2.714 CPU sec, 2.955 real sec...
sorting block 90, #reads = 1095749 mem = 536877577 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308058, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.58, 136.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730050 reads in 86.145 CPU sec, 2.803 real sec
[M::process] read 729698 sequences (100000144 bp) in 3.050 CPU sec, 3.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308029, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.73, 135.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729698 reads in 85.658 CPU sec, 2.784 real sec
[M::process] read 729770 sequences (100000049 bp) in 2.907 CPU sec, 3.056 real sec...
sorting block 91, #reads = 1094945 mem = 536878894 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308455, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 175, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 339)
[M::mem_pestat] mean and std.dev: (180.33, 36.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 397)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.10, 136.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 253, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (255.11, 88.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729770 reads in 90.463 CPU sec, 2.962 real sec
[M::process] read 729826 sequences (100000235 bp) in 2.883 CPU sec, 3.134 real sec...
sorting block 92, #reads = 1095231 mem = 536880942 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308231, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.50, 136.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729826 reads in 88.937 CPU sec, 2.891 real sec
[M::process] read 730480 sequences (100000128 bp) in 2.743 CPU sec, 2.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 308421, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 180, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (177.35, 50.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.02, 134.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730480 reads in 91.463 CPU sec, 2.971 real sec
[M::process] read 731030 sequences (100000019 bp) in 3.465 CPU sec, 3.657 real sec...
sorting block 93, #reads = 1095377 mem = 536878709 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309258, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (264.79, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731030 reads in 92.320 CPU sec, 2.991 real sec
[M::process] read 729948 sequences (100000271 bp) in 3.125 CPU sec, 3.317 real sec...
sorting block 94, #reads = 1095394 mem = 536878470 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308303, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.65, 136.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 154, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (167.10, 68.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 518)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729948 reads in 90.416 CPU sec, 2.945 real sec
[M::process] read 729622 sequences (100000110 bp) in 3.322 CPU sec, 3.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308200, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 198, 1351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3757)
[M::mem_pestat] mean and std.dev: (328.11, 371.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (269.38, 136.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 189, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 302)
[M::mem_pestat] mean and std.dev: (154.36, 38.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 357)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729622 reads in 89.912 CPU sec, 2.910 real sec
[M::process] read 729568 sequences (100000106 bp) in 2.851 CPU sec, 2.975 real sec...
sorting block 95, #reads = 1095248 mem = 536878896 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307625, 12, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (270.74, 137.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 83, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1683)
[M::mem_pestat] mean and std.dev: (243.67, 254.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2220)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 729568 reads in 91.736 CPU sec, 2.997 real sec
[M::process] read 729112 sequences (100000255 bp) in 3.912 CPU sec, 4.196 real sec...
sorting block 96, #reads = 1094700 mem = 536876822 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307765, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (273.34, 139.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728786 sequences (100000136 bp) in 2.791 CPU sec, 2.894 real sec...
[M::mem_process_seqs] Processed 729112 reads in 91.460 CPU sec, 2.957 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307783, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 177, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (204.07, 107.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (274.46, 139.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728786 reads in 89.085 CPU sec, 2.901 real sec
sorting block 97, #reads = 1093917 mem = 536878933 peak = 1005924352
[M::process] read 728680 sequences (100000115 bp) in 3.556 CPU sec, 3.778 real sec...
[M::process] read 728432 sequences (100000074 bp) in 2.377 CPU sec, 2.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307986, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.47, 140.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (99, 164, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393)
[M::mem_pestat] mean and std.dev: (152.56, 56.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] skip orientation RR
[M::process] read 728708 sequences (100000053 bp) in 1.710 CPU sec, 1.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307359, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.74, 139.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728680 reads in 167.643 CPU sec, 5.277 real sec
[M::mem_process_seqs] Processed 728432 reads in 101.688 CPU sec, 3.209 real sec
[M::process] read 728664 sequences (100000208 bp) in 2.307 CPU sec, 2.347 real sec...
sorting block 98, #reads = 1094324 mem = 536884177 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307515, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (276.46, 141.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307616, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 215, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (203.40, 73.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (276.77, 142.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728004 sequences (100000141 bp) in 3.493 CPU sec, 3.757 real sec...
[M::mem_process_seqs] Processed 728708 reads in 169.513 CPU sec, 5.433 real sec
[M::mem_process_seqs] Processed 728664 reads in 101.598 CPU sec, 3.237 real sec
sorting block 99, #reads = 1093455 mem = 536879401 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306960, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (277.09, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728004 reads in 89.128 CPU sec, 2.932 real sec
sorting block 100, #reads = 1094613 mem = 536878781 peak = 1005924352
[M::process] read 728214 sequences (100000243 bp) in 3.327 CPU sec, 3.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307279, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (277.79, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 184, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 360)
[M::mem_pestat] mean and std.dev: (187.73, 34.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728214 reads in 90.932 CPU sec, 2.981 real sec
sorting block 101, #reads = 1093202 mem = 536881191 peak = 1005924352
[M::process] read 728402 sequences (100000058 bp) in 3.655 CPU sec, 4.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307331, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 178, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 293)
[M::mem_pestat] mean and std.dev: (166.36, 28.11)
[M::mem_pestat] low and high boundaries for proper pairs: (35, 336)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (277.36, 143.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728402 reads in 89.042 CPU sec, 2.888 real sec
[M::process] read 731878 sequences (100000096 bp) in 2.957 CPU sec, 3.083 real sec...
sorting block 102, #reads = 1093561 mem = 536877229 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309349, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (267.20, 139.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731878 reads in 86.691 CPU sec, 2.828 real sec
[M::process] read 732738 sequences (100000097 bp) in 3.690 CPU sec, 4.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309198, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (262.94, 135.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731660 sequences (100000091 bp) in 2.565 CPU sec, 2.689 real sec...
[M::mem_process_seqs] Processed 732738 reads in 88.452 CPU sec, 2.855 real sec
sorting block 103, #reads = 1096032 mem = 536876859 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309376, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (265.58, 136.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731660 reads in 89.412 CPU sec, 2.900 real sec
[M::process] read 731478 sequences (100000264 bp) in 3.214 CPU sec, 3.377 real sec...
sorting block 104, #reads = 1096960 mem = 536881379 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308663, 9, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 191, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (189.25, 69.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.26, 135.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 182, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (222.67, 117.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731478 reads in 91.889 CPU sec, 2.987 real sec
[M::process] read 731088 sequences (100000122 bp) in 2.946 CPU sec, 3.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308861, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.10, 136.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731088 reads in 90.664 CPU sec, 2.947 real sec
[M::process] read 730652 sequences (100000272 bp) in 2.838 CPU sec, 2.988 real sec...
sorting block 105, #reads = 1096794 mem = 536881020 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308384, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 266, 599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559)
[M::mem_pestat] mean and std.dev: (323.56, 274.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2039)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.78, 136.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730652 reads in 90.842 CPU sec, 2.949 real sec
[M::process] read 730398 sequences (100000087 bp) in 2.789 CPU sec, 2.952 real sec...
sorting block 106, #reads = 1095867 mem = 536880670 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308714, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.77, 136.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 343)
[M::mem_pestat] mean and std.dev: (176.88, 32.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 402)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730398 reads in 90.008 CPU sec, 2.921 real sec
[M::process] read 730798 sequences (100000123 bp) in 3.388 CPU sec, 3.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308488, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 187, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (183.20, 42.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.49, 137.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730798 reads in 90.435 CPU sec, 2.929 real sec
[M::process] read 729978 sequences (100000124 bp) in 2.743 CPU sec, 2.992 real sec...
sorting block 107, #reads = 1095588 mem = 536882414 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308187, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.81, 136.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 230, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.27, 122.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RR
[M::process] read 730246 sequences (100000257 bp) in 2.747 CPU sec, 2.877 real sec...
[M::mem_process_seqs] Processed 729978 reads in 90.117 CPU sec, 2.941 real sec
sorting block 108, #reads = 1095254 mem = 536879096 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308863, 7, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.68, 136.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (196.31, 55.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 559)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730246 reads in 88.584 CPU sec, 2.874 real sec
[M::process] read 730524 sequences (100000230 bp) in 3.730 CPU sec, 4.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308333, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 147, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (104, 194)
[M::mem_pestat] mean and std.dev: (142.78, 13.30)
[M::mem_pestat] low and high boundaries for proper pairs: (86, 212)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.29, 137.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730524 reads in 91.619 CPU sec, 2.966 real sec
[M::process] read 731554 sequences (100000152 bp) in 3.208 CPU sec, 3.354 real sec...
sorting block 109, #reads = 1095468 mem = 536880199 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 308506, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 222, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (217.60, 87.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.62, 134.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 185, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (175.70, 47.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731554 reads in 93.895 CPU sec, 3.054 real sec
[M::process] read 732220 sequences (100000150 bp) in 3.027 CPU sec, 3.183 real sec...
sorting block 110, #reads = 1095770 mem = 536878448 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309062, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (261.78, 133.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732220 reads in 90.683 CPU sec, 2.947 real sec
[M::process] read 731516 sequences (100000090 bp) in 3.126 CPU sec, 3.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 309292, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 197, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 369)
[M::mem_pestat] mean and std.dev: (192.06, 40.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 438)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.61, 134.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 192, 1116)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2984)
[M::mem_pestat] mean and std.dev: (506.20, 709.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3918)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730636 sequences (100000232 bp) in 2.419 CPU sec, 2.632 real sec...
[M::mem_process_seqs] Processed 731516 reads in 91.412 CPU sec, 2.962 real sec
sorting block 111, #reads = 1096751 mem = 536878940 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308529, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (266.78, 136.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730080 sequences (100000128 bp) in 2.958 CPU sec, 3.131 real sec...
[M::mem_process_seqs] Processed 730636 reads in 88.689 CPU sec, 2.869 real sec
sorting block 112, #reads = 1096190 mem = 536879421 peak = 1005924352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308411, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.54, 137.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 207, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (220.00, 100.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730080 reads in 84.170 CPU sec, 2.730 real sec
[M::process] read 729706 sequences (100000257 bp) in 3.092 CPU sec, 3.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307924, 7, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 197, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (106, 291)
[M::mem_pestat] mean and std.dev: (199.86, 16.08)
[M::mem_pestat] low and high boundaries for proper pairs: (69, 328)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (269.97, 137.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 206, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (314.75, 259.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1414)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729706 reads in 86.493 CPU sec, 2.810 real sec
sorting block 113, #reads = 1092890 mem = 536878948 peak = 1005924352
[M::process] read 729236 sequences (100000199 bp) in 3.550 CPU sec, 3.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307755, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (273.12, 139.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728484 sequences (100000233 bp) in 2.613 CPU sec, 2.761 real sec...
[M::mem_process_seqs] Processed 729236 reads in 90.648 CPU sec, 2.934 real sec
sorting block 114, #reads = 1094415 mem = 536878127 peak = 1005924352
[M::process] read 728276 sequences (100000265 bp) in 2.545 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307685, 10, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 190, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 303)
[M::mem_pestat] mean and std.dev: (180.91, 54.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (274.41, 139.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (70, 728, 1579)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4597)
[M::mem_pestat] mean and std.dev: (985.90, 1226.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6106)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 184, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 275)
[M::mem_pestat] mean and std.dev: (169.78, 41.48)
[M::mem_pestat] low and high boundaries for proper pairs: (4, 336)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 728150 sequences (100000166 bp) in 2.177 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307631, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.23, 139.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 198, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (176.67, 48.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728484 reads in 168.795 CPU sec, 5.332 real sec
[M::mem_process_seqs] Processed 728276 reads in 100.984 CPU sec, 3.204 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307069, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 167, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (233.67, 156.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (276.94, 141.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 115, #reads = 1093999 mem = 536879379 peak = 1005924352
[M::mem_process_seqs] Processed 728150 reads in 89.979 CPU sec, 3.001 real sec
[M::process] read 728020 sequences (100000056 bp) in 3.760 CPU sec, 4.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307265, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (277.07, 141.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728020 reads in 86.922 CPU sec, 2.892 real sec
sorting block 116, #reads = 1093491 mem = 536877770 peak = 1005924352
[M::process] read 728104 sequences (100000235 bp) in 3.716 CPU sec, 4.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307367, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (278.58, 143.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (144.50, 47.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 482)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728104 reads in 89.903 CPU sec, 2.938 real sec
sorting block 117, #reads = 1093556 mem = 536878972 peak = 1014771712
[M::process] read 728330 sequences (100000156 bp) in 3.553 CPU sec, 3.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307138, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 201, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (188.30, 72.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 575)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (277.69, 143.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728388 sequences (100000089 bp) in 2.649 CPU sec, 2.745 real sec...
[M::mem_process_seqs] Processed 728330 reads in 86.570 CPU sec, 2.794 real sec
sorting block 118, #reads = 1093316 mem = 536877697 peak = 1014771712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307252, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (278.09, 144.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 211, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (177.89, 55.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 566)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728388 reads in 88.731 CPU sec, 2.889 real sec
[M::process] read 731196 sequences (100000154 bp) in 3.597 CPU sec, 3.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308370, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 191, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390)
[M::mem_pestat] mean and std.dev: (169.27, 49.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 243, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (269.02, 140.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731196 reads in 87.705 CPU sec, 2.852 real sec
sorting block 119, #reads = 1093314 mem = 536882709 peak = 1014771712
[M::process] read 732542 sequences (100000078 bp) in 3.537 CPU sec, 3.825 real sec...
[M::process] read 731496 sequences (100000187 bp) in 2.158 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309274, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (263.96, 135.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731208 sequences (100000202 bp) in 1.570 CPU sec, 1.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308498, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 150, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (176.60, 63.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (266.11, 136.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732542 reads in 162.282 CPU sec, 5.108 real sec
[M::mem_process_seqs] Processed 731496 reads in 96.471 CPU sec, 3.049 real sec
[M::process] read 730822 sequences (100000035 bp) in 2.120 CPU sec, 2.190 real sec...
sorting block 120, #reads = 1096277 mem = 536881003 peak = 1014771712
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308135, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.45, 135.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 139, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (192.64, 69.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation RR
[M::process] read 730634 sequences (100000261 bp) in 2.442 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308844, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.72, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731208 reads in 151.101 CPU sec, 4.836 real sec
sorting block 121, #reads = 1096455 mem = 536878217 peak = 1014771712
[M::mem_process_seqs] Processed 730822 reads in 87.682 CPU sec, 2.783 real sec
[M::process] read 730406 sequences (100000026 bp) in 2.623 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308543, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.23, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 171, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 322)
[M::mem_pestat] mean and std.dev: (164.58, 44.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 384)
[M::mem_pestat] skip orientation RR
sorting block 122, #reads = 1095177 mem = 536877813 peak = 1022316544
[M::process] read 730880 sequences (100000270 bp) in 2.210 CPU sec, 2.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308474, 5, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.58, 136.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (107, 136, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (164.15, 69.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730634 reads in 138.408 CPU sec, 4.425 real sec
[M::mem_process_seqs] Processed 730406 reads in 80.891 CPU sec, 2.572 real sec
[M::process] read 729938 sequences (100000062 bp) in 2.357 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308315, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.11, 136.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 123, #reads = 1095154 mem = 536878595 peak = 1022316544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307818, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (268.24, 135.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 161, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (184.27, 82.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730880 reads in 132.731 CPU sec, 4.288 real sec
[M::process] read 730364 sequences (100000185 bp) in 2.380 CPU sec, 2.611 real sec...
[M::mem_process_seqs] Processed 729938 reads in 82.861 CPU sec, 2.665 real sec
sorting block 124, #reads = 1095300 mem = 536879874 peak = 1022316544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 308238, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.71, 135.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731034 sequences (100000285 bp) in 2.440 CPU sec, 2.627 real sec...
[M::mem_process_seqs] Processed 730364 reads in 80.812 CPU sec, 2.673 real sec
sorting block 125, #reads = 1095556 mem = 536879302 peak = 1022316544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308944, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 287, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229)
[M::mem_pestat] mean and std.dev: (327.90, 153.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1570)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.43, 135.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731034 reads in 77.435 CPU sec, 2.566 real sec
[M::process] read 731608 sequences (100000098 bp) in 3.035 CPU sec, 3.352 real sec...
sorting block 126, #reads = 1095358 mem = 536878198 peak = 1022316544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 309449, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 223, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (196.21, 80.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 239, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (262.57, 133.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731608 reads in 80.652 CPU sec, 2.610 real sec
[M::process] read 731778 sequences (100000171 bp) in 2.542 CPU sec, 2.658 real sec...
sorting block 127, #reads = 1096049 mem = 536878424 peak = 1022316544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308943, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (262.00, 133.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 181, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (224.92, 88.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation RR
[M::process] read 731890 sequences (100000143 bp) in 2.439 CPU sec, 2.525 real sec...
[M::mem_process_seqs] Processed 731778 reads in 79.935 CPU sec, 2.591 real sec
[M::process] read 731442 sequences (100000224 bp) in 2.057 CPU sec, 2.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309407, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 149, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (189.10, 98.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (262.18, 133.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731438 sequences (100000032 bp) in 1.500 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309177, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 239, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (262.74, 133.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731890 reads in 146.007 CPU sec, 4.601 real sec
[M::mem_process_seqs] Processed 731442 reads in 87.072 CPU sec, 2.757 real sec
[M::process] read 730990 sequences (100000226 bp) in 1.781 CPU sec, 1.831 real sec...
sorting block 128, #reads = 1096198 mem = 536880747 peak = 1022316544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 309353, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 159, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (164.69, 65.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 477)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (263.21, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 244, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (265.43, 141.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 129, #reads = 1095236 mem = 536880492 peak = 1054646272
[M::process] read 730300 sequences (100000033 bp) in 2.201 CPU sec, 2.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308709, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (264.20, 134.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731438 reads in 150.210 CPU sec, 4.780 real sec
[M::process] read 730172 sequences (100000218 bp) in 1.653 CPU sec, 1.661 real sec...
[M::mem_process_seqs] Processed 730990 reads in 89.448 CPU sec, 2.835 real sec
sorting block 130, #reads = 1095666 mem = 536878807 peak = 1054646272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308299, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.23, 135.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (331, 381, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 757)
[M::mem_pestat] mean and std.dev: (308.00, 111.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RR
[M::process] read 728898 sequences (100000183 bp) in 1.904 CPU sec, 1.955 real sec...
[M::process] read 728544 sequences (100000006 bp) in 1.500 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307957, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 243, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (198.44, 61.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.71, 137.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 182, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (235.00, 154.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730300 reads in 145.459 CPU sec, 4.645 real sec
[M::mem_process_seqs] Processed 730172 reads in 86.808 CPU sec, 2.763 real sec
sorting block 131, #reads = 1094612 mem = 536881262 peak = 1054646272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308169, 1, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 213, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (209.00, 80.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (270.31, 136.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 150, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 294)
[M::mem_pestat] mean and std.dev: (146.57, 41.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 350)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728196 sequences (100000002 bp) in 2.418 CPU sec, 2.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307434, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.27, 139.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728898 reads in 145.040 CPU sec, 4.617 real sec
[M::mem_process_seqs] Processed 728544 reads in 86.941 CPU sec, 2.750 real sec
sorting block 132, #reads = 1094077 mem = 536879524 peak = 1107173376
[M::process] read 727258 sequences (100000093 bp) in 2.534 CPU sec, 2.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307254, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (275.95, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 223, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (282.10, 123.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RR
sorting block 133, #reads = 1093984 mem = 536880169 peak = 1107173376
[M::process] read 727588 sequences (100000188 bp) in 2.486 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306511, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.00, 140.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728196 reads in 148.208 CPU sec, 4.760 real sec
[M::mem_process_seqs] Processed 727258 reads in 87.672 CPU sec, 2.787 real sec
[M::process] read 727892 sequences (100000231 bp) in 2.432 CPU sec, 2.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306803, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 191, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (233.67, 138.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (278.49, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 134, #reads = 1093755 mem = 536880801 peak = 1107173376
[M::process] read 623146 sequences (85601165 bp) in 1.910 CPU sec, 1.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306802, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 204, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (213.88, 95.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.23, 142.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727588 reads in 145.895 CPU sec, 4.681 real sec
sorting block 135, #reads = 1093225 mem = 536878025 peak = 1107173376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 262498, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (279.41, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 623146 reads in 62.631 CPU sec, 2.175 real sec
[M::mem_process_seqs] Processed 727892 reads in 148.129 CPU sec, 5.523 real sec
sorting block 136, #reads = 1093322 mem = 536881007 peak = 1107173376
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 149451346
overall: 69797724160 mem 18897.576 user 782.855 sys 689.414 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.1_25PercentHG514.L002\tPU:L002\tSM:1_25PercentHG514\tLB:1_25PercentHG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1_25PercentHG514_R1.fastq.gz 0003.1_25PercentHG514_R2.fastq.gz
[main] Real time: 689.477 sec; CPU: 19680.457 sec
sorting block 137, #reads = 318042 mem = 156291076 peak = 1107173376
uniq header count 1
merging 138 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 6544255876
output reads: 150296585
bam_mem_sort: 138 calls 81.433 user 2.878 sys 84.508 real
bam_write: 138 calls 51.115 user 2.514 sys 54.128 real
execute: 1 calls 33.394 user 1.821 sys 728.901 real
merge_files: 1 calls 33.366 user 1.794 sys 39.017 real
parse_chunk: 2197 calls 121.980 user 27.631 sys 151.559 real
read_chunk: 5955 calls 60.813 user 39.090 sys 651.155 real
sort_block: 1 calls 45.799 user 38.683 sys 629.731 real
write_chunk: 3756 calls 32.893 user 1.568 sys 38.003 real
overall: 2773102592 mem 1523.167 user 90.835 sys 728.922 real
ls: cannot access '*.cram': No such file or directory