File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f3/66e368b3ed7feeb7e0ab11f13135db/.command.log
Size
194.2 KB
Task
DAQ:SENTIEON_BWAMEM (SH032_HG514;L007;0001)
Attempt

Content

View in new tab 
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f3/66e368b3ed7feeb7e0ab11f13135db/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/60/0e277621fd007f69d60167a6bdf14a/SH032_HG514/0001.SH032_HG514_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/60/0e277621fd007f69d60167a6bdf14a/SH032_HG514/0001.SH032_HG514_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f3/66e368b3ed7feeb7e0ab11f13135db/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o SH032_HG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1487.034 CPU sec, 50.772 real sec
[M::process] read 728394 sequences (100000193 bp) in 0.947 CPU sec, 0.951 real sec...
[M::process] read 727176 sequences (100000169 bp) in 1.464 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306902, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 223, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 327)
[M::mem_pestat] mean and std.dev: (194.33, 41.30)
[M::mem_pestat] low and high boundaries for proper pairs: (15, 379)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.99, 142.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 202, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 320)
[M::mem_pestat] mean and std.dev: (186.82, 57.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726336 sequences (100000194 bp) in 1.501 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306481, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 206, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (203.71, 83.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 726096 sequences (100000272 bp) in 1.484 CPU sec, 1.500 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.72, 142.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 237, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (215.67, 86.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728394 reads in 149.004 CPU sec, 4.682 real sec
[M::mem_process_seqs] Processed 727176 reads in 83.539 CPU sec, 2.652 real sec
sorting block 0, #reads = 1104587 mem = 536879082 peak = 624013312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306519, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 217, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (194.76, 66.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.72, 143.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726012 sequences (100000002 bp) in 1.512 CPU sec, 1.995 real sec...
[M::process] read 725980 sequences (100000279 bp) in 1.376 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 306002, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 173, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (190.10, 65.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 655)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.83, 142.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 216, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 352)
[M::mem_pestat] mean and std.dev: (199.91, 52.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 417)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726336 reads in 145.083 CPU sec, 4.740 real sec
[M::mem_process_seqs] Processed 726096 reads in 83.528 CPU sec, 2.707 real sec
sorting block 1, #reads = 1103469 mem = 536884243 peak = 785494016
[M::process] read 726068 sequences (100000008 bp) in 1.689 CPU sec, 1.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306281, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 166, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 282)
[M::mem_pestat] mean and std.dev: (178.24, 49.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 375)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.94, 144.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 257, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (223.76, 78.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725712 sequences (100000261 bp) in 1.576 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306041, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 151, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390)
[M::mem_pestat] mean and std.dev: (153.18, 47.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.91, 142.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 257, 2131)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6153)
[M::mem_pestat] mean and std.dev: (927.00, 1188.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8164)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726012 reads in 154.849 CPU sec, 5.034 real sec
[M::mem_process_seqs] Processed 725980 reads in 88.564 CPU sec, 2.843 real sec
sorting block 2, #reads = 1103249 mem = 536878805 peak = 936235008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306041, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 197, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (208.92, 62.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 772)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.91, 143.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (259.28, 114.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726284 sequences (100000014 bp) in 1.603 CPU sec, 1.803 real sec...
[M::process] read 727336 sequences (100000274 bp) in 1.465 CPU sec, 1.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306279, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 216, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (182.80, 67.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 585)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (283.93, 144.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 135, 161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 247)
[M::mem_pestat] mean and std.dev: (134.11, 22.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 290)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726068 reads in 156.083 CPU sec, 5.076 real sec
sorting block 3, #reads = 1102623 mem = 536880963 peak = 936235008
[M::mem_process_seqs] Processed 725712 reads in 88.180 CPU sec, 2.826 real sec
sorting block 4, #reads = 1103117 mem = 536879256 peak = 1024557056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306080, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 176, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (198.37, 89.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.24, 142.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 274, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (234.38, 82.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727412 sequences (100000184 bp) in 1.520 CPU sec, 1.843 real sec...
[M::process] read 727524 sequences (100000079 bp) in 1.539 CPU sec, 1.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306951, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 218, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 382)
[M::mem_pestat] mean and std.dev: (206.06, 50.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (278.27, 142.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 167, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (186.05, 77.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726284 reads in 155.138 CPU sec, 5.085 real sec
[M::mem_process_seqs] Processed 727336 reads in 87.444 CPU sec, 2.847 real sec
sorting block 5, #reads = 1103673 mem = 536877333 peak = 1024557056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307145, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 203, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297)
[M::mem_pestat] mean and std.dev: (276.47, 167.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1681)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (276.94, 141.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 206, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (191.21, 63.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727056 sequences (100000045 bp) in 1.592 CPU sec, 1.991 real sec...
[M::process] read 725490 sequences (100000105 bp) in 1.449 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306335, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 228, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (267.57, 169.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (276.50, 141.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727412 reads in 157.003 CPU sec, 5.106 real sec
[M::mem_process_seqs] Processed 727524 reads in 88.991 CPU sec, 2.868 real sec
sorting block 6, #reads = 1104237 mem = 536878894 peak = 1024557056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306663, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 233, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (198.95, 78.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.78, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 194, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (191.73, 61.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724076 sequences (100000164 bp) in 1.599 CPU sec, 1.857 real sec...
[M::process] read 723452 sequences (100000286 bp) in 1.523 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305479, 8, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 172, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (196.72, 85.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.48, 145.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 184, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435)
[M::mem_pestat] mean and std.dev: (197.00, 86.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727056 reads in 158.049 CPU sec, 5.142 real sec
sorting block 7, #reads = 1104252 mem = 536879179 peak = 1053335552
[M::mem_process_seqs] Processed 725490 reads in 89.673 CPU sec, 2.878 real sec
sorting block 8, #reads = 1103655 mem = 536877946 peak = 1070235648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305178, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 213, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (221.32, 89.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.40, 147.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 722716 sequences (100000002 bp) in 1.542 CPU sec, 2.071 real sec...
[M::process] read 726302 sequences (100000124 bp) in 1.479 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304914, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (233.61, 90.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 273, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (296.32, 151.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (175.33, 47.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724076 reads in 155.410 CPU sec, 5.104 real sec
[M::mem_process_seqs] Processed 723452 reads in 88.102 CPU sec, 2.876 real sec
sorting block 9, #reads = 1101561 mem = 536879949 peak = 1070235648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 303895, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 221, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (217.91, 80.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 276, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (298.87, 152.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 206, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (210.50, 75.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727798 sequences (100000236 bp) in 1.550 CPU sec, 1.964 real sec...
[M::process] read 726860 sequences (100000213 bp) in 1.411 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306113, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (197.04, 67.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 534)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (285.04, 146.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 237, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (233.27, 91.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1417)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722716 reads in 156.309 CPU sec, 5.084 real sec
[M::mem_process_seqs] Processed 726302 reads in 88.386 CPU sec, 2.849 real sec
sorting block 10, #reads = 1100357 mem = 536878667 peak = 1070235648
[M::process] read 726692 sequences (100000040 bp) in 1.654 CPU sec, 2.033 real sec...
[M::process] read 726264 sequences (100000271 bp) in 1.389 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306818, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 210, 4167)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12177)
[M::mem_pestat] mean and std.dev: (1177.62, 1793.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16182)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (279.58, 142.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 196, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (206.62, 68.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726860 reads in 82.609 CPU sec, 2.637 real sec
sorting block 11, #reads = 1102321 mem = 536879539 peak = 1091919872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 307080, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 180, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (188.50, 92.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.00, 142.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 204, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (211.36, 79.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306720, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 211, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (195.75, 55.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 646)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (279.09, 141.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 180, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (197.10, 96.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727798 reads in 237.655 CPU sec, 7.749 real sec
[M::process] read 726346 sequences (100000139 bp) in 1.341 CPU sec, 1.575 real sec...
sorting block 12, #reads = 1104278 mem = 536880450 peak = 1091919872
[M::process] read 725998 sequences (100000029 bp) in 1.375 CPU sec, 1.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306508, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 178, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (56, 271)
[M::mem_pestat] mean and std.dev: (165.33, 31.47)
[M::mem_pestat] low and high boundaries for proper pairs: (13, 314)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (280.99, 142.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 219, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (211.08, 53.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726264 reads in 82.765 CPU sec, 2.714 real sec
[M::mem_process_seqs] Processed 726692 reads in 203.359 CPU sec, 6.682 real sec
sorting block 13, #reads = 1104071 mem = 536879159 peak = 1091919872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306618, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 188, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 420)
[M::mem_pestat] mean and std.dev: (196.17, 65.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 502)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (282.13, 143.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 303, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (100, 520)
[M::mem_pestat] mean and std.dev: (290.17, 41.00)
[M::mem_pestat] low and high boundaries for proper pairs: (16, 604)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725958 sequences (100000248 bp) in 1.745 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305958, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 188, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (187.50, 87.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.78, 142.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 205, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (212.89, 77.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726346 reads in 159.883 CPU sec, 5.193 real sec
[M::process] read 725854 sequences (100000050 bp) in 1.331 CPU sec, 1.431 real sec...
[M::mem_process_seqs] Processed 725998 reads in 85.828 CPU sec, 2.776 real sec
sorting block 14, #reads = 1103508 mem = 536880005 peak = 1091919872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306268, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 212, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435)
[M::mem_pestat] mean and std.dev: (203.73, 56.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 527)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (282.41, 143.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 174, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (182.77, 64.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726332 sequences (100000001 bp) in 1.515 CPU sec, 2.186 real sec...
[M::process] read 726698 sequences (100000092 bp) in 1.400 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306464, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 223, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425)
[M::mem_pestat] mean and std.dev: (209.95, 65.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.90, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 169, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (190.05, 74.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725958 reads in 148.175 CPU sec, 4.811 real sec
sorting block 15, #reads = 1102983 mem = 536880097 peak = 1121538048
[M::mem_process_seqs] Processed 725854 reads in 84.062 CPU sec, 2.697 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306789, 8, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 173, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 371)
[M::mem_pestat] mean and std.dev: (179.06, 51.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.53, 143.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 202, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (207.36, 121.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1102368 mem = 536879060 peak = 1129467904
[M::process] read 726092 sequences (100000096 bp) in 1.549 CPU sec, 1.997 real sec...
[M::process] read 726482 sequences (100000060 bp) in 1.348 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306496, 5, 31)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (280.31, 143.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 200, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (252.14, 135.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726332 reads in 148.594 CPU sec, 4.877 real sec
[M::mem_process_seqs] Processed 726698 reads in 84.462 CPU sec, 2.764 real sec
sorting block 17, #reads = 1104143 mem = 536876706 peak = 1129467904
[M::process] read 725148 sequences (100000214 bp) in 1.535 CPU sec, 2.037 real sec...
[M::process] read 724100 sequences (100000244 bp) in 1.457 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306478, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 264, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (231.71, 84.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.92, 143.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 229, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (217.62, 95.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726482 reads in 79.388 CPU sec, 2.549 real sec
sorting block 18, #reads = 1103301 mem = 536878365 peak = 1129467904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306082, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 177, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (200.40, 109.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.70, 143.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 152, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (30, 325)
[M::mem_pestat] mean and std.dev: (171.53, 47.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 384)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305507, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 230, 796)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2096)
[M::mem_pestat] mean and std.dev: (443.65, 516.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2746)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.25, 145.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 243, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (245.81, 75.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726092 reads in 227.449 CPU sec, 7.430 real sec
[M::process] read 724024 sequences (100000258 bp) in 1.350 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 725148 reads in 92.512 CPU sec, 3.073 real sec
sorting block 19, #reads = 1103456 mem = 536878336 peak = 1129467904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305704, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 166, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (198.77, 73.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (291.39, 148.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 205, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (186.07, 70.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723386 sequences (100000220 bp) in 1.624 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305343, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 165, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 387)
[M::mem_pestat] mean and std.dev: (172.83, 50.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (293.47, 149.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 210, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (190.00, 53.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724100 reads in 147.610 CPU sec, 4.809 real sec
[M::process] read 723212 sequences (100000100 bp) in 1.374 CPU sec, 1.381 real sec...
[M::mem_process_seqs] Processed 724024 reads in 84.591 CPU sec, 2.722 real sec
sorting block 20, #reads = 1101872 mem = 536878840 peak = 1129467904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305051, 5, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 157, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (173.12, 68.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (294.27, 148.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 165, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (172.08, 66.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728180 sequences (100000015 bp) in 1.575 CPU sec, 1.866 real sec...
[M::process] read 727418 sequences (100000039 bp) in 1.343 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304606, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 213, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (192.50, 82.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (292.95, 147.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 237, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (248.62, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723386 reads in 148.024 CPU sec, 4.810 real sec
[M::mem_process_seqs] Processed 723212 reads in 85.029 CPU sec, 2.721 real sec
sorting block 21, #reads = 1101036 mem = 536878640 peak = 1135505408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307291, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 232, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (216.14, 67.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 667)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (278.90, 144.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 168, 181)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (73, 253)
[M::mem_pestat] mean and std.dev: (155.78, 31.56)
[M::mem_pestat] low and high boundaries for proper pairs: (30, 289)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1100648 mem = 536877677 peak = 1155055616
[M::process] read 726656 sequences (100000082 bp) in 1.654 CPU sec, 2.216 real sec...
[M::process] read 726268 sequences (100000117 bp) in 1.459 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306539, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (216.87, 84.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 581)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.87, 142.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 188, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (189.00, 63.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728180 reads in 148.047 CPU sec, 4.878 real sec
[M::mem_process_seqs] Processed 727418 reads in 85.171 CPU sec, 2.798 real sec
sorting block 23, #reads = 1104268 mem = 536877352 peak = 1155055616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306464, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 186, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449)
[M::mem_pestat] mean and std.dev: (188.73, 72.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.76, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 206, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (228.75, 130.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726144 sequences (100000237 bp) in 1.671 CPU sec, 1.814 real sec...
[M::process] read 726046 sequences (100000063 bp) in 1.343 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306446, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 162, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (178.15, 97.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (280.72, 142.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 253, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300)
[M::mem_pestat] mean and std.dev: (287.93, 164.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1675)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726656 reads in 148.633 CPU sec, 4.842 real sec
[M::mem_process_seqs] Processed 726268 reads in 84.729 CPU sec, 2.745 real sec
sorting block 24, #reads = 1103741 mem = 536877741 peak = 1155055616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306134, 1, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 154, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339)
[M::mem_pestat] mean and std.dev: (164.90, 48.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 409)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.16, 144.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 156, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (173.04, 48.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 443)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725502 sequences (100000178 bp) in 1.609 CPU sec, 1.868 real sec...
[M::process] read 725708 sequences (100000018 bp) in 1.348 CPU sec, 1.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306203, 5, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 189, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377)
[M::mem_pestat] mean and std.dev: (175.06, 55.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.29, 145.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 174, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (220.81, 96.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 651)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726144 reads in 147.860 CPU sec, 4.811 real sec
[M::mem_process_seqs] Processed 726046 reads in 84.077 CPU sec, 2.693 real sec
sorting block 25, #reads = 1102925 mem = 536877764 peak = 1155055616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305988, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 159, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364)
[M::mem_pestat] mean and std.dev: (178.67, 66.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (284.29, 144.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 253, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (241.36, 90.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 26, #reads = 1103356 mem = 536877931 peak = 1173430272
[M::process] read 725270 sequences (100000251 bp) in 1.671 CPU sec, 2.040 real sec...
[M::process] read 725360 sequences (100000054 bp) in 1.378 CPU sec, 1.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306265, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 172, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (189.67, 72.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (284.87, 144.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 189, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (178.88, 61.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 442)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725502 reads in 149.303 CPU sec, 4.906 real sec
[M::mem_process_seqs] Processed 725708 reads in 84.976 CPU sec, 2.778 real sec
sorting block 27, #reads = 1103190 mem = 536877973 peak = 1173430272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305581, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 259, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500)
[M::mem_pestat] mean and std.dev: (214.80, 74.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (283.93, 143.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 175, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (200.76, 71.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725086 sequences (100000194 bp) in 1.733 CPU sec, 1.832 real sec...
[M::process] read 725150 sequences (100000214 bp) in 1.350 CPU sec, 1.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 305862, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (285.50, 145.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 210, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (188.70, 56.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725270 reads in 149.020 CPU sec, 4.856 real sec
[M::mem_process_seqs] Processed 725360 reads in 85.036 CPU sec, 2.756 real sec
sorting block 28, #reads = 1102507 mem = 536877486 peak = 1173430272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305597, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 169, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (176.94, 56.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (284.65, 143.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 724948 sequences (100000073 bp) in 1.586 CPU sec, 2.115 real sec...
[M::process] read 724010 sequences (100000022 bp) in 1.438 CPU sec, 1.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305895, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 229, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (220.57, 78.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (284.43, 144.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 199, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (214.75, 68.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725086 reads in 149.361 CPU sec, 4.853 real sec
[M::mem_process_seqs] Processed 725150 reads in 85.300 CPU sec, 2.729 real sec
sorting block 29, #reads = 1102676 mem = 536880256 peak = 1189945344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305313, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 177, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (82, 272)
[M::mem_pestat] mean and std.dev: (171.38, 35.90)
[M::mem_pestat] low and high boundaries for proper pairs: (28, 315)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (286.32, 145.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 179, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (220.00, 120.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 30, #reads = 1102128 mem = 536877442 peak = 1189945344
[M::process] read 724004 sequences (100000030 bp) in 1.659 CPU sec, 2.075 real sec...
[M::process] read 723478 sequences (100000081 bp) in 1.381 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305651, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 177, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 343)
[M::mem_pestat] mean and std.dev: (192.00, 56.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (292.97, 149.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 201, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 367)
[M::mem_pestat] mean and std.dev: (192.15, 39.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724948 reads in 148.135 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 724010 reads in 84.021 CPU sec, 2.749 real sec
sorting block 31, #reads = 1101510 mem = 536880590 peak = 1189945344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304965, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 189, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 324)
[M::mem_pestat] mean and std.dev: (195.93, 62.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 445)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (292.59, 148.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 225, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435)
[M::mem_pestat] mean and std.dev: (211.05, 74.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 722976 sequences (100000104 bp) in 1.709 CPU sec, 1.822 real sec...
[M::process] read 724630 sequences (100000221 bp) in 1.355 CPU sec, 1.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305147, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 251, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (238.78, 90.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 272, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (293.36, 147.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 245, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (240.93, 72.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724004 reads in 149.312 CPU sec, 4.870 real sec
[M::mem_process_seqs] Processed 723478 reads in 85.497 CPU sec, 2.784 real sec
sorting block 32, #reads = 1101805 mem = 536878361 peak = 1189945344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304404, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 166, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (202.18, 81.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (294.17, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 222, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (187.50, 89.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727638 sequences (100000130 bp) in 1.640 CPU sec, 1.907 real sec...
[M::process] read 726654 sequences (100000231 bp) in 1.356 CPU sec, 1.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304729, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 211, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (196.20, 65.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (289.37, 146.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 223, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (204.27, 57.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 663)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722976 reads in 151.600 CPU sec, 4.940 real sec
[M::mem_process_seqs] Processed 724630 reads in 87.611 CPU sec, 2.823 real sec
sorting block 33, #reads = 1100730 mem = 536878909 peak = 1189945344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306975, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 208, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473)
[M::mem_pestat] mean and std.dev: (236.20, 90.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.51, 145.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 224, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (42, 407)
[M::mem_pestat] mean and std.dev: (214.71, 60.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726232 sequences (100000012 bp) in 1.645 CPU sec, 1.936 real sec...
sorting block 34, #reads = 1100516 mem = 536879164 peak = 1204948992
[M::process] read 725966 sequences (100000139 bp) in 1.310 CPU sec, 1.598 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306251, 11, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 253, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (258.62, 120.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (281.91, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 70, 124)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286)
[M::mem_pestat] mean and std.dev: (73.80, 50.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 259, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420)
[M::mem_pestat] mean and std.dev: (393.57, 384.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1932)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727638 reads in 148.996 CPU sec, 4.909 real sec
[M::mem_process_seqs] Processed 726654 reads in 84.878 CPU sec, 2.788 real sec
sorting block 35, #reads = 1104154 mem = 536878929 peak = 1204948992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306385, 8, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 191, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (198.58, 75.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (283.94, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 233, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (259.00, 132.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725590 sequences (100000122 bp) in 1.689 CPU sec, 1.773 real sec...
[M::process] read 725292 sequences (100000192 bp) in 1.348 CPU sec, 1.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306231, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 199, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 334)
[M::mem_pestat] mean and std.dev: (170.27, 51.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.45, 144.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 212, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 427)
[M::mem_pestat] mean and std.dev: (232.07, 89.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726232 reads in 148.991 CPU sec, 4.845 real sec
[M::mem_process_seqs] Processed 725966 reads in 84.810 CPU sec, 2.756 real sec
sorting block 36, #reads = 1103242 mem = 536877644 peak = 1204948992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 305781, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.08, 145.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 146, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (196.00, 95.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 663)
[M::mem_pestat] skip orientation RR
[M::process] read 724922 sequences (100000079 bp) in 1.627 CPU sec, 1.702 real sec...
[M::process] read 724644 sequences (100000054 bp) in 1.316 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305487, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 173, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (177.32, 58.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (287.27, 146.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 168, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (177.59, 63.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 547)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725590 reads in 147.405 CPU sec, 4.791 real sec
[M::mem_process_seqs] Processed 725292 reads in 83.642 CPU sec, 2.689 real sec
sorting block 37, #reads = 1102809 mem = 536884807 peak = 1204948992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305584, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 203, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (186.17, 51.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 549)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (286.97, 145.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (229.38, 73.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724080 sequences (100000272 bp) in 1.665 CPU sec, 1.858 real sec...
sorting block 38, #reads = 1102496 mem = 536876626 peak = 1222193152
[M::process] read 723906 sequences (100000014 bp) in 1.316 CPU sec, 1.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305645, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 198, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 347)
[M::mem_pestat] mean and std.dev: (178.13, 49.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 410)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (287.45, 145.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 228, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (192.14, 71.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724922 reads in 146.898 CPU sec, 4.848 real sec
[M::mem_process_seqs] Processed 724644 reads in 82.899 CPU sec, 2.736 real sec
sorting block 39, #reads = 1102209 mem = 536877392 peak = 1222193152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305713, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 213, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461)
[M::mem_pestat] mean and std.dev: (203.06, 79.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (289.87, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 232, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (237.38, 54.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723540 sequences (100000221 bp) in 1.650 CPU sec, 1.804 real sec...
[M::process] read 723848 sequences (100000154 bp) in 1.340 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304988, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 209, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434)
[M::mem_pestat] mean and std.dev: (201.60, 65.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (289.12, 145.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 724080 reads in 147.057 CPU sec, 4.799 real sec
[M::mem_process_seqs] Processed 723906 reads in 84.170 CPU sec, 2.743 real sec
sorting block 40, #reads = 1102008 mem = 536878982 peak = 1222193152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305159, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 159, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (153.93, 39.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 270, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (291.47, 146.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 240, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (215.13, 103.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723120 sequences (100000130 bp) in 1.600 CPU sec, 2.015 real sec...
[M::process] read 723056 sequences (100000236 bp) in 1.365 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305091, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 261, 934)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2494)
[M::mem_pestat] mean and std.dev: (518.65, 480.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3274)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (291.84, 147.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 193, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (166.54, 52.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 499)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723540 reads in 147.908 CPU sec, 4.816 real sec
[M::mem_process_seqs] Processed 723848 reads in 84.531 CPU sec, 2.722 real sec
sorting block 41, #reads = 1101108 mem = 536880815 peak = 1230438400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304757, 1, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 188, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (188.55, 55.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (294.04, 148.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 152, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (193.80, 83.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 651)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723254 sequences (100000157 bp) in 1.646 CPU sec, 2.158 real sec...
sorting block 42, #reads = 1100825 mem = 536879125 peak = 1230528512
[M::process] read 722822 sequences (100000168 bp) in 1.403 CPU sec, 1.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305071, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491)
[M::mem_pestat] mean and std.dev: (189.36, 43.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (295.05, 148.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 231, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (222.91, 91.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723120 reads in 147.569 CPU sec, 4.839 real sec
[M::mem_process_seqs] Processed 723056 reads in 84.811 CPU sec, 2.768 real sec
sorting block 43, #reads = 1100285 mem = 536878112 peak = 1230528512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304299, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 184, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 322)
[M::mem_pestat] mean and std.dev: (177.57, 41.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 380)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 272, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (293.58, 147.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 228, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (270.13, 121.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 722466 sequences (100000206 bp) in 1.581 CPU sec, 2.103 real sec...
[M::process] read 726466 sequences (100000041 bp) in 1.477 CPU sec, 1.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 304570, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 221, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (229.95, 104.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 274, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (295.82, 148.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 221, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (280.33, 193.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723254 reads in 150.108 CPU sec, 4.904 real sec
[M::mem_process_seqs] Processed 722822 reads in 86.072 CPU sec, 2.792 real sec
sorting block 44, #reads = 1100424 mem = 536876576 peak = 1230528512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304199, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 185, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (201.32, 82.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 277, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (298.47, 150.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 198, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (192.16, 67.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 684)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728300 sequences (100000249 bp) in 1.687 CPU sec, 2.236 real sec...
[M::process] read 727174 sequences (100000003 bp) in 1.398 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305902, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 174, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350)
[M::mem_pestat] mean and std.dev: (158.50, 41.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 422)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (285.24, 147.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 235, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (205.88, 74.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722466 reads in 149.775 CPU sec, 4.880 real sec
[M::mem_process_seqs] Processed 726466 reads in 86.640 CPU sec, 2.777 real sec
sorting block 45, #reads = 1100376 mem = 536876313 peak = 1230528512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306875, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 233, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (247.71, 99.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (279.16, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 209, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431)
[M::mem_pestat] mean and std.dev: (189.92, 77.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 531)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727080 sequences (100000125 bp) in 1.577 CPU sec, 1.981 real sec...
[M::process] read 726626 sequences (100000115 bp) in 1.505 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306621, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 209, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353)
[M::mem_pestat] mean and std.dev: (193.00, 57.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (281.66, 144.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 185, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (192.19, 62.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728300 reads in 148.567 CPU sec, 4.826 real sec
sorting block 46, #reads = 1101764 mem = 536878950 peak = 1230995456
[M::mem_process_seqs] Processed 727174 reads in 83.124 CPU sec, 2.655 real sec
sorting block 47, #reads = 1104071 mem = 536879033 peak = 1240858624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306428, 0, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 202, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (199.59, 56.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.82, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 145, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (170.33, 78.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725998 sequences (100000032 bp) in 1.572 CPU sec, 1.857 real sec...
[M::process] read 725948 sequences (100000236 bp) in 1.357 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306156, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 210, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 468)
[M::mem_pestat] mean and std.dev: (240.44, 89.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.53, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 244, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (220.38, 83.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727080 reads in 154.167 CPU sec, 5.061 real sec
[M::mem_process_seqs] Processed 726626 reads in 87.460 CPU sec, 2.857 real sec
sorting block 48, #reads = 1103626 mem = 536876828 peak = 1240858624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305737, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 156, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (267.11, 216.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.40, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 185, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (44, 324)
[M::mem_pestat] mean and std.dev: (173.06, 45.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 380)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726508 sequences (100000081 bp) in 1.724 CPU sec, 2.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305714, 9, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 117, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (149.38, 64.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (284.71, 145.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 222, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (216.06, 68.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726538 sequences (100000241 bp) in 1.373 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 725998 reads in 153.201 CPU sec, 5.004 real sec
[M::mem_process_seqs] Processed 725948 reads in 87.050 CPU sec, 2.824 real sec
sorting block 49, #reads = 1102684 mem = 536880504 peak = 1240858624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305994, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 180, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (194.91, 84.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.58, 145.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 156, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (185.24, 84.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727260 sequences (100000203 bp) in 1.661 CPU sec, 2.004 real sec...
[M::process] read 728212 sequences (100000181 bp) in 1.374 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306501, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 155, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 282)
[M::mem_pestat] mean and std.dev: (147.56, 34.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 333)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.57, 143.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 186, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (197.60, 83.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726508 reads in 151.534 CPU sec, 4.925 real sec
sorting block 50, #reads = 1102766 mem = 536879553 peak = 1241792512
[M::mem_process_seqs] Processed 726538 reads in 85.900 CPU sec, 2.748 real sec
sorting block 51, #reads = 1102831 mem = 536878852 peak = 1241792512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306132, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 175, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (190.41, 81.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.55, 143.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 186, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (190.50, 59.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 541)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728264 sequences (100000000 bp) in 1.683 CPU sec, 1.953 real sec...
[M::process] read 728218 sequences (100000018 bp) in 1.387 CPU sec, 1.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307176, 1, 26)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (275.62, 141.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 155, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 295)
[M::mem_pestat] mean and std.dev: (162.68, 44.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 351)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727260 reads in 155.696 CPU sec, 5.107 real sec
[M::mem_process_seqs] Processed 728212 reads in 86.806 CPU sec, 2.830 real sec
sorting block 52, #reads = 1103041 mem = 536879775 peak = 1241792512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307073, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 259, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (242.75, 126.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (276.09, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 221, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (198.67, 61.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726152 sequences (100000259 bp) in 1.680 CPU sec, 2.099 real sec...
[M::process] read 724670 sequences (100000123 bp) in 1.373 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307072, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 215, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (259.93, 146.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (277.14, 143.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 209, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.00, 66.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728264 reads in 152.389 CPU sec, 4.960 real sec
[M::mem_process_seqs] Processed 728218 reads in 86.764 CPU sec, 2.790 real sec
sorting block 53, #reads = 1104228 mem = 536880803 peak = 1241792512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 305802, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 222, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (212.56, 70.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (284.45, 146.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 187, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (178.91, 55.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724232 sequences (100000096 bp) in 1.716 CPU sec, 1.820 real sec...
[M::process] read 724110 sequences (100000186 bp) in 1.382 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305401, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 214, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580)
[M::mem_pestat] mean and std.dev: (194.46, 65.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (288.98, 147.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 195, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (203.24, 76.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726152 reads in 154.491 CPU sec, 5.031 real sec
sorting block 54, #reads = 1104354 mem = 536879046 peak = 1244119040
[M::mem_process_seqs] Processed 724670 reads in 87.073 CPU sec, 2.787 real sec
sorting block 55, #reads = 1101995 mem = 536877029 peak = 1244438528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305525, 7, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 255, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (331.92, 246.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1374)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (293.23, 150.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (226.73, 83.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726908 sequences (100000028 bp) in 1.674 CPU sec, 1.909 real sec...
[M::process] read 728204 sequences (100000042 bp) in 1.345 CPU sec, 1.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 304834, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 156, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413)
[M::mem_pestat] mean and std.dev: (161.00, 47.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 507)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (293.10, 149.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 214, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417)
[M::mem_pestat] mean and std.dev: (200.53, 76.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 509)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724232 reads in 154.308 CPU sec, 5.064 real sec
[M::mem_process_seqs] Processed 724110 reads in 88.023 CPU sec, 2.875 real sec
sorting block 56, #reads = 1101518 mem = 536880368 peak = 1244438528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305869, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 280, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (211.23, 96.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (284.27, 147.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 179, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (220.28, 86.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728044 sequences (100000036 bp) in 1.721 CPU sec, 1.860 real sec...
[M::process] read 727444 sequences (100000016 bp) in 1.378 CPU sec, 1.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306888, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 208, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (250.17, 124.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (277.43, 143.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 246, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (191.50, 67.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726908 reads in 152.261 CPU sec, 4.955 real sec
[M::mem_process_seqs] Processed 728204 reads in 86.155 CPU sec, 2.780 real sec
sorting block 57, #reads = 1101345 mem = 536877347 peak = 1244438528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 307019, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 211, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (197.21, 75.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 501)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (280.16, 144.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 181, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (184.17, 49.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 452)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726538 sequences (100000167 bp) in 1.637 CPU sec, 2.017 real sec...
[M::process] read 726202 sequences (100000097 bp) in 1.355 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306722, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 202, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (184.90, 64.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (280.88, 144.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 207, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (199.92, 77.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728044 reads in 150.456 CPU sec, 4.886 real sec
sorting block 58, #reads = 1104558 mem = 536877721 peak = 1251610624
[M::mem_process_seqs] Processed 727444 reads in 85.911 CPU sec, 2.745 real sec
sorting block 59, #reads = 1104276 mem = 536881053 peak = 1251688448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306364, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 171, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 293)
[M::mem_pestat] mean and std.dev: (170.76, 53.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 383)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.97, 143.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 211, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (199.58, 61.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726114 sequences (100000109 bp) in 1.630 CPU sec, 2.193 real sec...
[M::process] read 726216 sequences (100000240 bp) in 1.495 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305530, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 184, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (163.27, 43.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.71, 143.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (107, 163, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (153.31, 59.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 455)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726538 reads in 150.859 CPU sec, 4.950 real sec
[M::mem_process_seqs] Processed 726202 reads in 87.122 CPU sec, 2.836 real sec
sorting block 60, #reads = 1103597 mem = 536879290 peak = 1251688448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306251, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 224, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (216.79, 79.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.97, 144.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 178, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (185.27, 83.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726038 sequences (100000219 bp) in 1.665 CPU sec, 1.806 real sec...
[M::process] read 726866 sequences (100000043 bp) in 1.353 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305939, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 222, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (235.23, 97.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (283.66, 144.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 207, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (236.83, 100.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726114 reads in 150.849 CPU sec, 4.916 real sec
[M::mem_process_seqs] Processed 726216 reads in 85.764 CPU sec, 2.779 real sec
sorting block 61, #reads = 1102830 mem = 536878291 peak = 1251688448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306217, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 195, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407)
[M::mem_pestat] mean and std.dev: (186.93, 63.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.34, 145.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 175, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338)
[M::mem_pestat] mean and std.dev: (167.82, 46.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 407)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726938 sequences (100000038 bp) in 1.616 CPU sec, 2.218 real sec...
[M::process] read 726992 sequences (100000104 bp) in 1.474 CPU sec, 1.479 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306077, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 131, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374)
[M::mem_pestat] mean and std.dev: (137.00, 33.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.33, 143.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 177, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (190.95, 85.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726038 reads in 151.620 CPU sec, 4.925 real sec
[M::mem_process_seqs] Processed 726866 reads in 86.843 CPU sec, 2.770 real sec
sorting block 62, #reads = 1103075 mem = 536880146 peak = 1263845376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306436, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 164, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (175.86, 73.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 546)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 63, #reads = 1103076 mem = 536878500 peak = 1263845376
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.18, 143.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 155, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (175.17, 51.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727116 sequences (100000133 bp) in 1.700 CPU sec, 1.877 real sec...
[M::process] read 725580 sequences (100000134 bp) in 1.348 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306149, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 220, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (206.06, 63.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 530)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (281.18, 144.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 195, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 301)
[M::mem_pestat] mean and std.dev: (191.64, 34.82)
[M::mem_pestat] low and high boundaries for proper pairs: (52, 342)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726938 reads in 153.587 CPU sec, 5.040 real sec
[M::mem_process_seqs] Processed 726992 reads in 87.279 CPU sec, 2.854 real sec
sorting block 64, #reads = 1103224 mem = 536879572 peak = 1263845376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307173, 0, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 218, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (163.62, 70.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (281.83, 145.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 201, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 362)
[M::mem_pestat] mean and std.dev: (205.43, 64.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724706 sequences (100000224 bp) in 1.720 CPU sec, 1.987 real sec...
[M::process] read 724648 sequences (100000287 bp) in 1.387 CPU sec, 1.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305207, 8, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 205, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (205.89, 64.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (287.48, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 183, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (177.75, 72.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727116 reads in 152.259 CPU sec, 4.960 real sec
[M::mem_process_seqs] Processed 725580 reads in 87.724 CPU sec, 2.833 real sec
sorting block 65, #reads = 1104118 mem = 536881273 peak = 1263845376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305288, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 141, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (185.62, 79.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 650)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (291.30, 149.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 240, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 412)
[M::mem_pestat] mean and std.dev: (224.31, 64.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723980 sequences (100000020 bp) in 1.675 CPU sec, 1.958 real sec...
[M::process] read 726794 sequences (100000033 bp) in 1.380 CPU sec, 1.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305060, 8, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 150, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 272)
[M::mem_pestat] mean and std.dev: (146.00, 26.48)
[M::mem_pestat] low and high boundaries for proper pairs: (20, 314)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (293.06, 151.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 167, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (171.28, 86.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724706 reads in 150.468 CPU sec, 4.894 real sec
[M::mem_process_seqs] Processed 724648 reads in 86.976 CPU sec, 2.792 real sec
sorting block 66, #reads = 1102823 mem = 536880414 peak = 1263845376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305046, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 192, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (225.38, 101.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 271, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (296.03, 152.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 331, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (285.29, 118.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 67, #reads = 1100552 mem = 536881590 peak = 1272815616
[M::process] read 728528 sequences (100000048 bp) in 1.648 CPU sec, 1.991 real sec...
[M::process] read 728074 sequences (100000023 bp) in 1.388 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306244, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 171, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (167.13, 60.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (285.26, 149.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 212, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (218.39, 82.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723980 reads in 152.169 CPU sec, 4.983 real sec
[M::mem_process_seqs] Processed 726794 reads in 87.595 CPU sec, 2.857 real sec
sorting block 68, #reads = 1101234 mem = 536878158 peak = 1272815616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306817, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 178, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 348)
[M::mem_pestat] mean and std.dev: (182.85, 45.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 412)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (276.86, 142.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 199, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (196.39, 60.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727236 sequences (100000202 bp) in 1.636 CPU sec, 2.058 real sec...
[M::process] read 726852 sequences (100000211 bp) in 1.383 CPU sec, 1.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306809, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 186, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531)
[M::mem_pestat] mean and std.dev: (205.67, 56.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.73, 143.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 229, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (228.71, 96.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728528 reads in 152.260 CPU sec, 4.955 real sec
[M::mem_process_seqs] Processed 728074 reads in 86.597 CPU sec, 2.799 real sec
sorting block 69, #reads = 1103832 mem = 536880153 peak = 1272815616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306310, 2, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 197, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 376)
[M::mem_pestat] mean and std.dev: (174.21, 59.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.92, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 181, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (288.12, 159.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726570 sequences (100000181 bp) in 1.634 CPU sec, 2.039 real sec...
[M::process] read 725864 sequences (100000001 bp) in 1.376 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306526, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (221.64, 122.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (283.08, 146.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (219.89, 96.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727236 reads in 151.335 CPU sec, 4.921 real sec
[M::mem_process_seqs] Processed 726852 reads in 86.067 CPU sec, 2.765 real sec
sorting block 70, #reads = 1104736 mem = 536881480 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306242, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 179, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461)
[M::mem_pestat] mean and std.dev: (202.43, 73.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (284.18, 145.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 243, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (235.55, 85.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 71, #reads = 1103510 mem = 536880965 peak = 1272852480
[M::process] read 725960 sequences (100000060 bp) in 1.593 CPU sec, 1.911 real sec...
[M::process] read 725574 sequences (100000278 bp) in 1.354 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305879, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 175, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (168.50, 50.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (284.00, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 223, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (237.21, 78.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726570 reads in 151.978 CPU sec, 4.995 real sec
[M::mem_process_seqs] Processed 725864 reads in 86.755 CPU sec, 2.828 real sec
sorting block 72, #reads = 1103129 mem = 536879018 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306081, 7, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 160, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (192.32, 79.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (285.01, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 175, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (206.95, 89.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725234 sequences (100000056 bp) in 1.697 CPU sec, 1.906 real sec...
[M::process] read 725704 sequences (100000253 bp) in 1.373 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305567, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 164, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 329)
[M::mem_pestat] mean and std.dev: (159.77, 30.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 394)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.10, 146.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (235.21, 113.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725960 reads in 152.123 CPU sec, 4.951 real sec
[M::mem_process_seqs] Processed 725574 reads in 86.622 CPU sec, 2.803 real sec
sorting block 73, #reads = 1102956 mem = 536878075 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305877, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 213, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (214.00, 88.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (285.37, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 215, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (203.15, 60.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726006 sequences (100000090 bp) in 1.634 CPU sec, 1.880 real sec...
[M::process] read 726148 sequences (100000238 bp) in 1.335 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305570, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 160, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344)
[M::mem_pestat] mean and std.dev: (169.43, 61.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.00, 146.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 193, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (183.05, 66.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725234 reads in 152.710 CPU sec, 4.977 real sec
[M::mem_process_seqs] Processed 725704 reads in 87.125 CPU sec, 2.808 real sec
sorting block 74, #reads = 1102541 mem = 536878781 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305938, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 175, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (190.20, 60.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 585)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.49, 144.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 148, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434)
[M::mem_pestat] mean and std.dev: (177.00, 81.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 75, #reads = 1102326 mem = 536879320 peak = 1272852480
[M::process] read 724896 sequences (100000105 bp) in 1.694 CPU sec, 2.021 real sec...
[M::process] read 724524 sequences (100000037 bp) in 1.380 CPU sec, 1.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306379, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 206, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (199.19, 71.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 547)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (284.38, 146.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 172, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308)
[M::mem_pestat] mean and std.dev: (152.33, 37.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 371)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726006 reads in 150.791 CPU sec, 4.967 real sec
[M::mem_process_seqs] Processed 726148 reads in 85.749 CPU sec, 2.807 real sec
sorting block 76, #reads = 1102750 mem = 536878518 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304784, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 243, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (215.88, 61.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (288.27, 146.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 173, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 347)
[M::mem_pestat] mean and std.dev: (174.71, 44.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724438 sequences (100000120 bp) in 1.675 CPU sec, 2.019 real sec...
[M::process] read 724344 sequences (100000014 bp) in 1.385 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304954, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 191, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (170.94, 54.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 581)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (290.48, 148.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 178, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (214.88, 103.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724896 reads in 153.952 CPU sec, 5.010 real sec
[M::mem_process_seqs] Processed 724524 reads in 88.011 CPU sec, 2.843 real sec
sorting block 77, #reads = 1102675 mem = 536879134 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 304605, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 203, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (226.45, 88.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (293.61, 151.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 167, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (182.88, 71.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725716 sequences (100000038 bp) in 1.682 CPU sec, 2.242 real sec...
[M::process] read 728818 sequences (100000279 bp) in 1.482 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 305179, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 290, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (296.88, 106.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 268, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (294.71, 152.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 207, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (197.81, 61.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 613)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724438 reads in 153.158 CPU sec, 4.975 real sec
[M::mem_process_seqs] Processed 724344 reads in 87.472 CPU sec, 2.796 real sec
sorting block 78, #reads = 1101446 mem = 536879300 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305715, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 151, 172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 278)
[M::mem_pestat] mean and std.dev: (156.29, 47.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 344)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (291.23, 152.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 200, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 332)
[M::mem_pestat] mean and std.dev: (186.07, 47.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 383)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 79, #reads = 1100420 mem = 536878588 peak = 1272852480
[M::process] read 727460 sequences (100000185 bp) in 1.603 CPU sec, 1.995 real sec...
[M::process] read 727336 sequences (100000136 bp) in 1.382 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307328, 9, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 166, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (238.22, 122.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (277.22, 143.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 208, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (205.69, 80.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725716 reads in 150.579 CPU sec, 4.953 real sec
[M::mem_process_seqs] Processed 728818 reads in 86.695 CPU sec, 2.830 real sec
sorting block 80, #reads = 1102729 mem = 536878381 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306782, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 194, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (203.00, 107.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.38, 143.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 170, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329)
[M::mem_pestat] mean and std.dev: (165.89, 51.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 401)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726502 sequences (100000108 bp) in 1.705 CPU sec, 1.805 real sec...
[M::process] read 726244 sequences (100000090 bp) in 1.378 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 307039, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 252, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (225.88, 94.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (280.12, 143.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 199, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (211.56, 83.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727460 reads in 152.104 CPU sec, 4.955 real sec
sorting block 81, #reads = 1104725 mem = 536881125 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306312, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 191, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (196.95, 87.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.57, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 143, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 270)
[M::mem_pestat] mean and std.dev: (146.54, 25.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 315)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725686 sequences (100000012 bp) in 1.430 CPU sec, 1.553 real sec...
[M::mem_process_seqs] Processed 726502 reads in 80.194 CPU sec, 2.630 real sec
[M::mem_process_seqs] Processed 727336 reads in 198.750 CPU sec, 6.486 real sec
sorting block 82, #reads = 1103867 mem = 536880391 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306110, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 199, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 361)
[M::mem_pestat] mean and std.dev: (188.71, 53.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (284.78, 146.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 243, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 403)
[M::mem_pestat] mean and std.dev: (203.11, 52.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725440 sequences (100000192 bp) in 1.755 CPU sec, 1.859 real sec...
[M::process] read 725428 sequences (100000008 bp) in 1.374 CPU sec, 1.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305570, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 246, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (238.50, 101.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.08, 146.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 211, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (219.50, 114.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726244 reads in 159.376 CPU sec, 5.184 real sec
[M::mem_process_seqs] Processed 725686 reads in 86.194 CPU sec, 2.802 real sec
sorting block 83, #reads = 1103251 mem = 536876920 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305307, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 209, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (188.33, 65.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (286.05, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 234, 1046)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2688)
[M::mem_pestat] mean and std.dev: (441.18, 409.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3509)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724828 sequences (100000136 bp) in 1.621 CPU sec, 1.811 real sec...
[M::process] read 724696 sequences (100000062 bp) in 1.352 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306312, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 243, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544)
[M::mem_pestat] mean and std.dev: (207.52, 70.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.88, 146.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 725440 reads in 148.630 CPU sec, 4.847 real sec
[M::mem_process_seqs] Processed 725428 reads in 84.000 CPU sec, 2.704 real sec
sorting block 84, #reads = 1102292 mem = 536878298 peak = 1272852480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305416, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 231, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 377)
[M::mem_pestat] mean and std.dev: (210.00, 34.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 443)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (287.46, 145.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 228, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (211.94, 77.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724700 sequences (100000058 bp) in 1.722 CPU sec, 1.909 real sec...
sorting block 85, #reads = 1102413 mem = 536879500 peak = 1276211200
[M::process] read 724942 sequences (100000160 bp) in 1.352 CPU sec, 1.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305199, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 152, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359)
[M::mem_pestat] mean and std.dev: (153.19, 55.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (289.12, 147.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 169, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (190.44, 88.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724828 reads in 151.635 CPU sec, 4.979 real sec
[M::mem_process_seqs] Processed 724696 reads in 86.722 CPU sec, 2.852 real sec
sorting block 86, #reads = 1101956 mem = 536879879 peak = 1276211200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305426, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 308, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (251.00, 111.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (288.96, 146.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 209, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425)
[M::mem_pestat] mean and std.dev: (187.40, 48.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724178 sequences (100000206 bp) in 1.664 CPU sec, 1.970 real sec...
[M::process] read 724088 sequences (100000286 bp) in 1.389 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 305469, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 227, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (213.73, 84.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 671)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (289.82, 148.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 230, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (268.39, 122.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724700 reads in 150.531 CPU sec, 4.909 real sec
[M::mem_process_seqs] Processed 724942 reads in 85.741 CPU sec, 2.774 real sec
sorting block 87, #reads = 1101351 mem = 536878825 peak = 1276211200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 304851, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 186, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468)
[M::mem_pestat] mean and std.dev: (171.11, 53.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 585)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.49, 146.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 196, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (219.06, 76.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724300 sequences (100000276 bp) in 1.697 CPU sec, 2.061 real sec...
[M::process] read 724006 sequences (100000163 bp) in 1.410 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305172, 10, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 177, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (172.85, 58.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 269, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (292.54, 149.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (105, 1024, 1024)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2862)
[M::mem_pestat] mean and std.dev: (553.00, 471.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3781)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 179, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (183.75, 60.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724178 reads in 151.125 CPU sec, 4.922 real sec
[M::mem_process_seqs] Processed 724088 reads in 85.899 CPU sec, 2.754 real sec
sorting block 88, #reads = 1101986 mem = 536877471 peak = 1276211200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305246, 6, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 156, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 333)
[M::mem_pestat] mean and std.dev: (168.38, 39.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 398)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 270, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (293.81, 150.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 244, 4412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12928)
[M::mem_pestat] mean and std.dev: (1408.09, 1896.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17186)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724246 sequences (100000043 bp) in 1.740 CPU sec, 1.827 real sec...
sorting block 89, #reads = 1101117 mem = 536877882 peak = 1276227584
[M::process] read 72434 sequences (10004227 bp) in 0.202 CPU sec, 0.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304312, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 210, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402)
[M::mem_pestat] mean and std.dev: (205.00, 65.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 270, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (294.02, 151.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 335, 1388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3748)
[M::mem_pestat] mean and std.dev: (629.31, 527.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4928)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724300 reads in 151.123 CPU sec, 4.970 real sec
[M::mem_process_seqs] Processed 724006 reads in 85.102 CPU sec, 2.791 real sec
sorting block 90, #reads = 1101242 mem = 536879745 peak = 1276227584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305205, 12, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 158, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (167.31, 77.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 477)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 270, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (296.23, 154.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 539, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792)
[M::mem_pestat] mean and std.dev: (402.36, 406.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2360)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 30453, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (298.79, 155.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 724246 reads in 85.141 CPU sec, 2.854 real sec
[M::mem_process_seqs] Processed 72434 reads in 14.756 CPU sec, 0.524 real sec
sorting block 91, #reads = 1101284 mem = 536881441 peak = 1276227584
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 100954614
overall: 69761454080 mem 12356.432 user 140.618 sys 416.524 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.SH032_HG514.L007\tPU:L007\tSM:SH032_HG514\tLB:SH032_HG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.SH032_HG514_R1.fastq.gz 0001.SH032_HG514_R2.fastq.gz
[main] Real time: 416.561 sec; CPU: 12497.056 sec
sorting block 92, #reads = 97715 mem = 47736613 peak = 1276227584
uniq header count 1
merging 93 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 32
algo: util-sort
license: sentieon:util=1
output file size: 4454702335
output reads: 101541888
bam_mem_sort: 93 calls 55.369 user 0.098 sys 60.357 real
bam_write: 93 calls 49.945 user 2.228 sys 130.986 real
execute: 1 calls 26.780 user 0.593 sys 444.637 real
merge_files: 1 calls 26.758 user 0.572 sys 28.046 real
parse_chunk: 1474 calls 63.231 user 16.499 sys 107.560 real
read_chunk: 3990 calls 32.170 user 18.025 sys 371.544 real
sort_block: 1 calls 23.055 user 17.494 sys 360.164 real
write_chunk: 2514 calls 26.420 user 0.432 sys 27.418 real
overall: 2774806528 mem 1062.566 user 41.324 sys 444.657 real
ls: cannot access '*.cram': No such file or directory