.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1_25PercentHG514;L004;0004)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/91/e99bce507f3b12f008589d8bba11b0/.command.err
Size: 210.3 KB
Task: DAQ:SENTIEON_BWAMEM (1_25PercentHG514;L004;0004)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1_25PercentHG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2947.660 CPU sec, 125.356 real sec
[M::process] read 732480 sequences (100000218 bp) in 1.037 CPU sec, 1.038 real sec...
[M::process] read 730982 sequences (100000082 bp) in 1.896 CPU sec, 1.910 real sec...
[M::process] read 731184 sequences (100000266 bp) in 1.857 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309816, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (262.20, 133.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 159, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432)
[M::mem_pestat] mean and std.dev: (188.25, 69.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 526)
[M::mem_pestat] skip orientation RR
[M::process] read 731254 sequences (100000211 bp) in 1.777 CPU sec, 1.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309040, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.28, 135.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 178, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1866)
[M::mem_pestat] mean and std.dev: (389.40, 376.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2438)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309296, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 154, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351)
[M::mem_pestat] mean and std.dev: (166.91, 55.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (265.58, 135.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732480 reads in 314.944 CPU sec, 10.239 real sec
[M::mem_process_seqs] Processed 730982 reads in 185.098 CPU sec, 6.097 real sec
[M::mem_process_seqs] Processed 731184 reads in 192.885 CPU sec, 6.388 real sec
sorting block 0, #reads = 1097116 mem = 536878094 peak = 598683648
[M::process] read 730800 sequences (100000176 bp) in 2.184 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308849, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 167, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 333)
[M::mem_pestat] mean and std.dev: (158.89, 32.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.05, 134.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 238, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (232.70, 104.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 1, #reads = 1096428 mem = 536878695 peak = 721367040
[M::process] read 730188 sequences (100000075 bp) in 1.673 CPU sec, 1.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308815, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (265.89, 134.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731254 reads in 162.112 CPU sec, 5.219 real sec
[M::mem_process_seqs] Processed 730800 reads in 91.425 CPU sec, 2.903 real sec
[M::process] read 730512 sequences (100000189 bp) in 1.540 CPU sec, 1.588 real sec...
sorting block 2, #reads = 1095732 mem = 536879550 peak = 721367040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308719, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.57, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730706 sequences (100000177 bp) in 1.874 CPU sec, 1.959 real sec...
[M::process] read 731596 sequences (100000162 bp) in 1.243 CPU sec, 1.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308761, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 160, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (194.67, 68.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.19, 135.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730188 reads in 157.716 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 730512 reads in 86.890 CPU sec, 2.786 real sec
sorting block 3, #reads = 1095527 mem = 536878793 peak = 736632832
[M::process] read 731382 sequences (100000116 bp) in 1.732 CPU sec, 1.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309135, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (267.03, 136.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 140, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350)
[M::mem_pestat] mean and std.dev: (149.20, 39.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] skip orientation RR
[M::process] read 731192 sequences (100000071 bp) in 1.430 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309581, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.55, 134.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 260, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160)
[M::mem_pestat] mean and std.dev: (277.09, 156.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1489)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309496, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 197, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450)
[M::mem_pestat] mean and std.dev: (199.00, 70.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.99, 134.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730706 reads in 229.413 CPU sec, 7.360 real sec
[M::mem_process_seqs] Processed 731596 reads in 159.882 CPU sec, 5.141 real sec
[M::mem_process_seqs] Processed 731382 reads in 166.841 CPU sec, 5.395 real sec
sorting block 4, #reads = 1096098 mem = 536878444 peak = 774156288
sorting block 5, #reads = 1096571 mem = 536878155 peak = 841306112
[M::process] read 731458 sequences (100000066 bp) in 2.035 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309065, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 165, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (182.18, 68.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 240, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (263.37, 133.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730988 sequences (100000138 bp) in 1.583 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309280, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.73, 134.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 228, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (176.62, 58.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 651)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731192 reads in 164.566 CPU sec, 5.254 real sec
[M::process] read 731528 sequences (100000098 bp) in 1.480 CPU sec, 1.475 real sec...
[M::mem_process_seqs] Processed 731458 reads in 92.277 CPU sec, 2.935 real sec
sorting block 6, #reads = 1096634 mem = 536880298 peak = 841306112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308707, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 139, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (192.00, 100.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 7, #reads = 1096599 mem = 536880133 peak = 841306112
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (264.52, 134.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731198 sequences (100000200 bp) in 2.151 CPU sec, 2.350 real sec...
[M::process] read 731228 sequences (100000256 bp) in 1.230 CPU sec, 1.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309095, 11, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.66, 134.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (74, 108, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613)
[M::mem_pestat] mean and std.dev: (239.30, 248.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2126)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 190, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (197.77, 65.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730988 reads in 165.255 CPU sec, 5.301 real sec
[M::mem_process_seqs] Processed 731528 reads in 94.075 CPU sec, 3.037 real sec
sorting block 8, #reads = 1096093 mem = 536879729 peak = 841306112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309422, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.98, 134.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 197, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (175.20, 53.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 483)
[M::mem_pestat] skip orientation RR
[M::process] read 731456 sequences (100000217 bp) in 2.317 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309327, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (263.36, 133.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731026 sequences (100000097 bp) in 1.202 CPU sec, 1.206 real sec...
[M::mem_process_seqs] Processed 731198 reads in 152.842 CPU sec, 4.908 real sec
sorting block 9, #reads = 1096545 mem = 536878658 peak = 841306112
[M::process] read 730682 sequences (100000232 bp) in 1.372 CPU sec, 1.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309347, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 239, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (263.27, 134.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731456 reads in 80.808 CPU sec, 2.689 real sec
[M::mem_process_seqs] Processed 731228 reads in 189.099 CPU sec, 6.168 real sec
sorting block 10, #reads = 1096538 mem = 536880447 peak = 841306112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309042, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.24, 134.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 200, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (215.90, 73.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation RR
[M::process] read 730162 sequences (100000007 bp) in 1.791 CPU sec, 1.865 real sec...
[M::process] read 729616 sequences (100000157 bp) in 1.150 CPU sec, 1.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308729, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 174, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (208.27, 76.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.04, 135.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 140, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (202.77, 139.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731026 reads in 158.690 CPU sec, 5.087 real sec
[M::mem_process_seqs] Processed 730682 reads in 84.689 CPU sec, 2.736 real sec
sorting block 11, #reads = 1096220 mem = 536880132 peak = 841306112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308360, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.54, 135.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (176.30, 85.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RR
[M::process] read 729304 sequences (100000055 bp) in 2.795 CPU sec, 3.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308446, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 159, 168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 264)
[M::mem_pestat] mean and std.dev: (134.88, 24.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 312)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.57, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730162 reads in 153.959 CPU sec, 4.934 real sec
[M::mem_process_seqs] Processed 729616 reads in 87.788 CPU sec, 2.821 real sec
[M::process] read 729082 sequences (100000061 bp) in 1.324 CPU sec, 1.371 real sec...
sorting block 12, #reads = 1095947 mem = 536881027 peak = 841306112
sorting block 13, #reads = 1094824 mem = 536877487 peak = 841306112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308331, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (270.84, 137.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (291.70, 192.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RR
[M::process] read 729278 sequences (100000018 bp) in 1.735 CPU sec, 1.920 real sec...
[M::process] read 726404 sequences (100000059 bp) in 1.330 CPU sec, 1.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307849, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.87, 138.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729304 reads in 148.101 CPU sec, 4.743 real sec
[M::mem_process_seqs] Processed 729082 reads in 83.809 CPU sec, 2.686 real sec
sorting block 14, #reads = 1094296 mem = 536878821 peak = 841306112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308228, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.69, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730636 sequences (100000204 bp) in 2.441 CPU sec, 2.592 real sec...
[M::process] read 732200 sequences (100000185 bp) in 1.520 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306647, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (279.23, 140.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 212, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (186.56, 62.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729278 reads in 151.877 CPU sec, 4.835 real sec
[M::mem_process_seqs] Processed 726404 reads in 85.829 CPU sec, 2.730 real sec
sorting block 15, #reads = 1094405 mem = 536878484 peak = 841306112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 308754, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 265, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (204.87, 86.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (266.43, 135.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (220.82, 104.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 731230 sequences (100000180 bp) in 2.031 CPU sec, 2.161 real sec...
[M::process] read 730892 sequences (100000024 bp) in 1.180 CPU sec, 1.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309547, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (262.44, 133.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (97, 151, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343)
[M::mem_pestat] mean and std.dev: (147.30, 54.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730636 reads in 147.892 CPU sec, 4.745 real sec
[M::mem_process_seqs] Processed 732200 reads in 82.207 CPU sec, 2.652 real sec
sorting block 16, #reads = 1092489 mem = 536878702 peak = 841306112
sorting block 17, #reads = 1096782 mem = 536878428 peak = 846893056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309192, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 203, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (174.20, 65.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.20, 134.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 298, 2124)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6040)
[M::mem_pestat] mean and std.dev: (1604.43, 2275.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10707)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 731288 sequences (100000041 bp) in 1.727 CPU sec, 1.847 real sec...
[M::process] read 730640 sequences (100000231 bp) in 1.190 CPU sec, 1.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309072, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.00, 135.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731230 reads in 139.017 CPU sec, 4.439 real sec
[M::mem_process_seqs] Processed 730892 reads in 76.766 CPU sec, 2.452 real sec
sorting block 18, #reads = 1096451 mem = 536878994 peak = 846893056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309272, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.22, 134.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730322 sequences (100000259 bp) in 1.541 CPU sec, 1.648 real sec...
[M::process] read 730192 sequences (100000162 bp) in 1.160 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308747, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.25, 135.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731288 reads in 138.927 CPU sec, 4.442 real sec
[M::mem_process_seqs] Processed 730640 reads in 78.099 CPU sec, 2.495 real sec
sorting block 19, #reads = 1095995 mem = 536879434 peak = 846893056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309215, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 245, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272)
[M::mem_pestat] mean and std.dev: (308.64, 192.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1630)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.63, 135.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729828 sequences (100000199 bp) in 2.178 CPU sec, 2.259 real sec...
[M::process] read 729850 sequences (100000247 bp) in 1.410 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308387, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 174, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (183.79, 69.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (268.00, 135.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 214, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (215.58, 62.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 650)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730322 reads in 140.252 CPU sec, 4.462 real sec
[M::mem_process_seqs] Processed 730192 reads in 78.450 CPU sec, 2.492 real sec
sorting block 20, #reads = 1095390 mem = 536876164 peak = 846893056
sorting block 21, #reads = 1095847 mem = 536877254 peak = 857948160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308310, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.74, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 158, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (166.20, 50.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RR
[M::process] read 729602 sequences (100000260 bp) in 1.753 CPU sec, 1.861 real sec...
[M::process] read 729424 sequences (100000001 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308629, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 187, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (196.78, 70.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.08, 136.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729828 reads in 141.509 CPU sec, 4.514 real sec
[M::mem_process_seqs] Processed 729850 reads in 78.823 CPU sec, 2.510 real sec
sorting block 22, #reads = 1095347 mem = 536878824 peak = 857948160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308865, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 161, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321)
[M::mem_pestat] mean and std.dev: (155.50, 41.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 386)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (270.00, 137.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729664 sequences (100000201 bp) in 2.267 CPU sec, 2.369 real sec...
[M::process] read 729640 sequences (100000114 bp) in 1.440 CPU sec, 1.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308671, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 156, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 331)
[M::mem_pestat] mean and std.dev: (182.36, 50.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 392)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.70, 136.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729602 reads in 142.455 CPU sec, 4.533 real sec
[M::mem_process_seqs] Processed 729424 reads in 79.574 CPU sec, 2.529 real sec
sorting block 23, #reads = 1095453 mem = 536878590 peak = 857948160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307955, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.83, 137.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 238, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (214.64, 85.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RR
[M::process] read 730084 sequences (100000080 bp) in 1.803 CPU sec, 1.935 real sec...
[M::process] read 729386 sequences (100000239 bp) in 1.160 CPU sec, 1.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308120, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.19, 137.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729664 reads in 144.011 CPU sec, 4.605 real sec
[M::mem_process_seqs] Processed 729640 reads in 80.374 CPU sec, 2.571 real sec
sorting block 24, #reads = 1094912 mem = 536879478 peak = 857948160
sorting block 25, #reads = 1094739 mem = 536880347 peak = 921513984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308298, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 209, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420)
[M::mem_pestat] mean and std.dev: (186.75, 44.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (270.25, 138.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 220, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (217.00, 80.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729526 sequences (100000135 bp) in 2.432 CPU sec, 2.537 real sec...
[M::process] read 729732 sequences (100000146 bp) in 1.340 CPU sec, 1.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308258, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (270.08, 136.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 213, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422)
[M::mem_pestat] mean and std.dev: (204.80, 59.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730084 reads in 143.139 CPU sec, 4.547 real sec
[M::mem_process_seqs] Processed 729386 reads in 79.696 CPU sec, 2.518 real sec
sorting block 26, #reads = 1095260 mem = 536879583 peak = 921513984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308678, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.54, 137.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 220, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (194.50, 68.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RR
[M::process] read 729246 sequences (100000087 bp) in 1.854 CPU sec, 1.986 real sec...
[M::process] read 729754 sequences (100000094 bp) in 1.330 CPU sec, 1.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308407, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (270.78, 138.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729526 reads in 144.291 CPU sec, 4.600 real sec
[M::mem_process_seqs] Processed 729732 reads in 79.632 CPU sec, 2.540 real sec
sorting block 27, #reads = 1094353 mem = 536879680 peak = 921513984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308241, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (270.78, 137.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729906 sequences (100000094 bp) in 2.211 CPU sec, 2.326 real sec...
[M::process] read 729234 sequences (100000153 bp) in 1.200 CPU sec, 1.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308405, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.06, 136.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 234, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544)
[M::mem_pestat] mean and std.dev: (186.20, 64.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729246 reads in 144.102 CPU sec, 4.589 real sec
[M::mem_process_seqs] Processed 729754 reads in 79.782 CPU sec, 2.533 real sec
sorting block 28, #reads = 1095122 mem = 536879825 peak = 921513984
sorting block 29, #reads = 1094915 mem = 536880561 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308711, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.01, 136.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 178, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (210.91, 77.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation RR
[M::process] read 728752 sequences (100000167 bp) in 1.862 CPU sec, 1.978 real sec...
[M::process] read 729482 sequences (100000065 bp) in 1.170 CPU sec, 1.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308457, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.08, 138.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 181, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (147, 217)
[M::mem_pestat] mean and std.dev: (187.00, 10.09)
[M::mem_pestat] low and high boundaries for proper pairs: (133, 231)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729906 reads in 139.237 CPU sec, 4.442 real sec
[M::mem_process_seqs] Processed 729234 reads in 77.072 CPU sec, 2.452 real sec
sorting block 30, #reads = 1094899 mem = 536877196 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307712, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 273, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144)
[M::mem_pestat] mean and std.dev: (286.00, 151.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (272.99, 138.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730342 sequences (100000266 bp) in 1.835 CPU sec, 1.957 real sec...
[M::process] read 729866 sequences (100000145 bp) in 1.240 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308050, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.18, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 182, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (272.33, 206.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1482)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728752 reads in 137.968 CPU sec, 4.409 real sec
[M::mem_process_seqs] Processed 729482 reads in 77.154 CPU sec, 2.468 real sec
sorting block 31, #reads = 1094674 mem = 536877421 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308987, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (268.14, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 167, 181)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 277)
[M::mem_pestat] mean and std.dev: (144.38, 27.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 325)
[M::mem_pestat] skip orientation RR
[M::process] read 733034 sequences (100000230 bp) in 2.000 CPU sec, 2.101 real sec...
[M::process] read 732226 sequences (100000127 bp) in 1.220 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308528, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.60, 137.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 107, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (103.00, 75.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 730342 reads in 139.510 CPU sec, 4.443 real sec
[M::mem_process_seqs] Processed 729866 reads in 78.217 CPU sec, 2.483 real sec
sorting block 32, #reads = 1095036 mem = 536879011 peak = 926498816
sorting block 33, #reads = 1094744 mem = 536879617 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 310141, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (260.26, 132.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731710 sequences (100000202 bp) in 1.990 CPU sec, 2.093 real sec...
[M::process] read 730952 sequences (100000041 bp) in 1.200 CPU sec, 1.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309663, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (261.62, 133.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733034 reads in 138.699 CPU sec, 4.422 real sec
[M::mem_process_seqs] Processed 732226 reads in 77.763 CPU sec, 2.476 real sec
sorting block 34, #reads = 1097247 mem = 536879013 peak = 926498816
[M::process] read 730990 sequences (100000037 bp) in 1.723 CPU sec, 1.821 real sec...
[M::process] read 730418 sequences (100000223 bp) in 1.210 CPU sec, 1.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309009, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (265.89, 135.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 545, 1184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3158)
[M::mem_pestat] mean and std.dev: (466.83, 363.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4145)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730952 reads in 74.326 CPU sec, 2.367 real sec
sorting block 35, #reads = 1095975 mem = 536879188 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309385, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (264.98, 135.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 170, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109)
[M::mem_pestat] mean and std.dev: (233.10, 136.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1427)
[M::mem_pestat] skip orientation RR
[M::process] read 730296 sequences (100000139 bp) in 1.297 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308935, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.44, 135.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731710 reads in 210.509 CPU sec, 6.717 real sec
[M::mem_process_seqs] Processed 730990 reads in 84.281 CPU sec, 2.716 real sec
sorting block 36, #reads = 1096764 mem = 536879178 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308477, 1, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.65, 135.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 228, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (311.69, 210.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RR
[M::process] read 729868 sequences (100000099 bp) in 2.011 CPU sec, 2.156 real sec...
[M::process] read 729784 sequences (100000014 bp) in 1.200 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308813, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 214, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (241.82, 96.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (268.29, 136.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 281, 1018)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2742)
[M::mem_pestat] mean and std.dev: (429.25, 393.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3604)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730418 reads in 139.154 CPU sec, 4.439 real sec
[M::mem_process_seqs] Processed 730296 reads in 77.722 CPU sec, 2.473 real sec
sorting block 37, #reads = 1095909 mem = 536877402 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308851, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 125, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 279)
[M::mem_pestat] mean and std.dev: (138.69, 42.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 334)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.33, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 167, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (193.42, 69.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729812 sequences (100000256 bp) in 1.795 CPU sec, 1.905 real sec...
[M::process] read 729468 sequences (100000191 bp) in 1.190 CPU sec, 1.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308268, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.34, 136.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729868 reads in 139.489 CPU sec, 4.450 real sec
sorting block 38, #reads = 1095154 mem = 536878476 peak = 926498816
[M::mem_process_seqs] Processed 729784 reads in 78.079 CPU sec, 2.496 real sec
sorting block 39, #reads = 1095226 mem = 536879472 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308149, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (270.44, 138.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729010 sequences (100000111 bp) in 1.886 CPU sec, 2.019 real sec...
[M::process] read 729158 sequences (100000055 bp) in 1.210 CPU sec, 1.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308398, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.64, 137.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729812 reads in 138.707 CPU sec, 4.425 real sec
[M::mem_process_seqs] Processed 729468 reads in 77.747 CPU sec, 2.478 real sec
sorting block 40, #reads = 1095229 mem = 536878705 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308224, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.13, 137.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728972 sequences (100000286 bp) in 1.816 CPU sec, 1.915 real sec...
[M::process] read 728592 sequences (100000211 bp) in 1.190 CPU sec, 1.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308266, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.96, 137.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729010 reads in 138.803 CPU sec, 4.431 real sec
[M::mem_process_seqs] Processed 729158 reads in 77.424 CPU sec, 2.471 real sec
sorting block 41, #reads = 1094425 mem = 536878688 peak = 926498816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307934, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (273.05, 138.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 237, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195)
[M::mem_pestat] mean and std.dev: (310.00, 237.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1527)
[M::mem_pestat] skip orientation RR
[M::process] read 728274 sequences (100000120 bp) in 1.762 CPU sec, 1.903 real sec...
[M::process] read 728610 sequences (100000132 bp) in 1.210 CPU sec, 1.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307829, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491)
[M::mem_pestat] mean and std.dev: (178.36, 56.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (272.41, 137.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728972 reads in 138.482 CPU sec, 4.422 real sec
sorting block 42, #reads = 1094417 mem = 536879558 peak = 936820736
[M::process] read 727570 sequences (100000051 bp) in 1.320 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307580, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (273.69, 137.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 194, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (226.77, 148.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728274 reads in 75.730 CPU sec, 2.522 real sec
sorting block 43, #reads = 1093949 mem = 536878257 peak = 936820736
[M::mem_process_seqs] Processed 728592 reads in 171.506 CPU sec, 5.591 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307629, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (274.95, 139.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 727654 sequences (100000076 bp) in 1.689 CPU sec, 1.808 real sec...
[M::process] read 727660 sequences (100000202 bp) in 1.240 CPU sec, 1.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307297, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.38, 140.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 70, 76)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 142)
[M::mem_pestat] mean and std.dev: (50.38, 25.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 175)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 728610 reads in 144.748 CPU sec, 4.608 real sec
sorting block 44, #reads = 1094095 mem = 536879502 peak = 936820736
[M::mem_process_seqs] Processed 727570 reads in 77.684 CPU sec, 2.476 real sec
sorting block 45, #reads = 1094117 mem = 536881294 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307240, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (276.81, 140.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 330, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (367.80, 168.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] skip orientation RR
[M::process] read 727508 sequences (100000114 bp) in 1.836 CPU sec, 1.924 real sec...
[M::process] read 727564 sequences (100000200 bp) in 1.230 CPU sec, 1.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306930, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 153, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (171.30, 55.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (276.58, 140.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727654 reads in 139.964 CPU sec, 4.462 real sec
[M::mem_process_seqs] Processed 727660 reads in 78.471 CPU sec, 2.508 real sec
sorting block 46, #reads = 1093423 mem = 536877293 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306939, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (278.39, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 218, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (212.81, 69.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] skip orientation RR
[M::process] read 727706 sequences (100000128 bp) in 1.705 CPU sec, 1.845 real sec...
[M::process] read 729246 sequences (100000160 bp) in 1.210 CPU sec, 1.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307006, 10, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 202, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (229.80, 78.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.37, 139.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (83, 96, 109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 161)
[M::mem_pestat] mean and std.dev: (98.57, 9.74)
[M::mem_pestat] low and high boundaries for proper pairs: (5, 187)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 727508 reads in 139.829 CPU sec, 4.463 real sec
[M::mem_process_seqs] Processed 727564 reads in 77.732 CPU sec, 2.479 real sec
sorting block 47, #reads = 1093318 mem = 536878610 peak = 936820736
[M::process] read 730274 sequences (100000115 bp) in 1.580 CPU sec, 1.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307183, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 157, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142)
[M::mem_pestat] mean and std.dev: (202.75, 124.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1477)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (276.35, 139.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 167, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 323)
[M::mem_pestat] mean and std.dev: (172.11, 23.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 381)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730772 sequences (100000124 bp) in 1.250 CPU sec, 1.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307848, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 190, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 326)
[M::mem_pestat] mean and std.dev: (181.87, 49.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 381)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (272.96, 139.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727706 reads in 139.595 CPU sec, 4.455 real sec
[M::mem_process_seqs] Processed 729246 reads in 76.986 CPU sec, 2.461 real sec
sorting block 48, #reads = 1093095 mem = 536878030 peak = 936820736
sorting block 49, #reads = 1093612 mem = 536879027 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309108, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 193, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (198.45, 83.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (269.08, 138.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732672 sequences (100000276 bp) in 1.854 CPU sec, 2.015 real sec...
[M::process] read 732028 sequences (100000012 bp) in 1.220 CPU sec, 1.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308528, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 385)
[M::mem_pestat] mean and std.dev: (218.50, 62.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.58, 136.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730274 reads in 138.717 CPU sec, 4.424 real sec
[M::mem_process_seqs] Processed 730772 reads in 77.167 CPU sec, 2.459 real sec
sorting block 50, #reads = 1095228 mem = 536879918 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309981, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (259.92, 132.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731300 sequences (100000260 bp) in 1.652 CPU sec, 1.769 real sec...
[M::process] read 730988 sequences (100000109 bp) in 1.240 CPU sec, 1.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309436, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 170, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 364)
[M::mem_pestat] mean and std.dev: (184.40, 58.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (261.42, 132.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732672 reads in 139.195 CPU sec, 4.443 real sec
[M::mem_process_seqs] Processed 732028 reads in 76.979 CPU sec, 2.459 real sec
sorting block 51, #reads = 1096222 mem = 536878032 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 308865, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 286, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (290.10, 102.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.98, 133.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 172, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 326)
[M::mem_pestat] mean and std.dev: (175.10, 57.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 405)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 731048 sequences (100000041 bp) in 1.924 CPU sec, 2.052 real sec...
[M::process] read 730182 sequences (100000069 bp) in 1.200 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308932, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.29, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (86, 91, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 212)
[M::mem_pestat] mean and std.dev: (103.20, 28.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 254)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731300 reads in 139.270 CPU sec, 4.443 real sec
sorting block 52, #reads = 1096991 mem = 536877382 peak = 936820736
[M::mem_process_seqs] Processed 730988 reads in 77.759 CPU sec, 2.478 real sec
sorting block 53, #reads = 1096389 mem = 536879430 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309191, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.06, 135.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730382 sequences (100000100 bp) in 1.762 CPU sec, 1.880 real sec...
[M::process] read 729968 sequences (100000051 bp) in 1.240 CPU sec, 1.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308574, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 197, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (184.55, 58.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (267.91, 135.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731048 reads in 139.706 CPU sec, 4.459 real sec
[M::mem_process_seqs] Processed 730182 reads in 78.041 CPU sec, 2.480 real sec
sorting block 54, #reads = 1095852 mem = 536878873 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308834, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.62, 134.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 225, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (212.64, 75.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation RR
[M::process] read 729740 sequences (100000096 bp) in 2.010 CPU sec, 2.131 real sec...
[M::process] read 729506 sequences (100000165 bp) in 1.220 CPU sec, 1.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308814, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 179, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (187.56, 41.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.31, 136.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730382 reads in 138.238 CPU sec, 4.404 real sec
[M::mem_process_seqs] Processed 729968 reads in 76.390 CPU sec, 2.431 real sec
sorting block 55, #reads = 1095778 mem = 536880441 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308723, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.21, 137.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729402 sequences (100000097 bp) in 1.821 CPU sec, 1.907 real sec...
[M::process] read 728978 sequences (100000032 bp) in 1.240 CPU sec, 1.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308208, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (270.92, 137.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 190, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (203.18, 86.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729740 reads in 139.206 CPU sec, 4.445 real sec
sorting block 56, #reads = 1095206 mem = 536879822 peak = 936820736
[M::mem_process_seqs] Processed 729506 reads in 77.971 CPU sec, 2.487 real sec
sorting block 57, #reads = 1095139 mem = 536879585 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308275, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (271.61, 138.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729250 sequences (100000001 bp) in 2.464 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308299, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 156, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (211.79, 143.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.26, 138.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728992 sequences (100000180 bp) in 1.299 CPU sec, 1.294 real sec...
[M::mem_process_seqs] Processed 729402 reads in 138.803 CPU sec, 4.411 real sec
[M::mem_process_seqs] Processed 728978 reads in 77.249 CPU sec, 2.441 real sec
sorting block 58, #reads = 1095068 mem = 536878074 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308145, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.23, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728162 sequences (100000229 bp) in 1.655 CPU sec, 1.752 real sec...
[M::process] read 727978 sequences (100000214 bp) in 1.240 CPU sec, 1.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307970, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.25, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729250 reads in 137.677 CPU sec, 4.396 real sec
[M::mem_process_seqs] Processed 728992 reads in 76.966 CPU sec, 2.457 real sec
sorting block 59, #reads = 1094793 mem = 536876601 peak = 936820736
sorting block 60, #reads = 1094589 mem = 536877243 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307296, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 251, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (272.49, 136.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 727634 sequences (100000276 bp) in 1.721 CPU sec, 1.846 real sec...
[M::process] read 726486 sequences (100000153 bp) in 1.210 CPU sec, 1.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307732, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 209, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (199.90, 53.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (274.21, 138.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 171, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (183.80, 57.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728162 reads in 138.304 CPU sec, 4.418 real sec
[M::mem_process_seqs] Processed 727978 reads in 76.417 CPU sec, 2.440 real sec
sorting block 61, #reads = 1093609 mem = 536876963 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307259, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (276.20, 139.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 168, 964)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2652)
[M::mem_pestat] mean and std.dev: (278.92, 307.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3496)
[M::mem_pestat] skip orientation RR
[M::process] read 726762 sequences (100000054 bp) in 1.648 CPU sec, 1.755 real sec...
[M::process] read 726040 sequences (100000247 bp) in 1.230 CPU sec, 1.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306645, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (278.98, 139.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 180, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (200.21, 69.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727634 reads in 139.405 CPU sec, 4.452 real sec
[M::mem_process_seqs] Processed 726486 reads in 78.125 CPU sec, 2.500 real sec
sorting block 62, #reads = 1093200 mem = 536879040 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306549, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (279.26, 140.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 725980 sequences (100000252 bp) in 1.939 CPU sec, 2.063 real sec...
[M::process] read 726518 sequences (100000013 bp) in 1.210 CPU sec, 1.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306699, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (281.02, 140.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 205, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 334)
[M::mem_pestat] mean and std.dev: (177.60, 40.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726762 reads in 139.226 CPU sec, 4.441 real sec
[M::mem_process_seqs] Processed 726040 reads in 77.704 CPU sec, 2.483 real sec
sorting block 63, #reads = 1092249 mem = 536879906 peak = 936820736
sorting block 64, #reads = 1092409 mem = 536878057 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306674, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 259, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (280.16, 139.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 726066 sequences (100000035 bp) in 1.718 CPU sec, 1.800 real sec...
[M::process] read 728278 sequences (100000218 bp) in 1.240 CPU sec, 1.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306625, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (280.12, 140.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 212, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (202.30, 80.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725980 reads in 137.961 CPU sec, 4.404 real sec
[M::mem_process_seqs] Processed 726518 reads in 77.126 CPU sec, 2.460 real sec
sorting block 65, #reads = 1092031 mem = 536878110 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306445, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 172, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 383)
[M::mem_pestat] mean and std.dev: (191.00, 59.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 457)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (281.76, 141.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728934 sequences (100000027 bp) in 1.813 CPU sec, 1.924 real sec...
[M::process] read 730696 sequences (100000059 bp) in 1.250 CPU sec, 1.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307837, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.68, 139.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726066 reads in 138.829 CPU sec, 4.428 real sec
[M::mem_process_seqs] Processed 728278 reads in 76.999 CPU sec, 2.455 real sec
sorting block 66, #reads = 1091931 mem = 536881380 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307534, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 140, 150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 226)
[M::mem_pestat] mean and std.dev: (130.67, 26.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 264)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.71, 138.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 196, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 348)
[M::mem_pestat] mean and std.dev: (194.20, 44.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 410)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 734092 sequences (100000007 bp) in 1.733 CPU sec, 1.825 real sec...
[M::process] read 733056 sequences (100000092 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308365, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.77, 136.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 158, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473)
[M::mem_pestat] mean and std.dev: (165.90, 52.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 591)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728934 reads in 140.983 CPU sec, 4.500 real sec
[M::mem_process_seqs] Processed 730696 reads in 79.602 CPU sec, 2.540 real sec
sorting block 67, #reads = 1094080 mem = 536880992 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 310292, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (260.80, 136.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1094883 mem = 536876934 peak = 936820736
[M::process] read 732966 sequences (100000213 bp) in 3.008 CPU sec, 3.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309740, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (263.21, 137.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 734092 reads in 142.742 CPU sec, 4.550 real sec
[M::mem_process_seqs] Processed 733056 reads in 81.110 CPU sec, 2.571 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309934, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (263.67, 137.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 238, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (233.44, 102.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RR
[M::process] read 732356 sequences (100000034 bp) in 3.151 CPU sec, 3.504 real sec...
[M::mem_process_seqs] Processed 732966 reads in 75.574 CPU sec, 2.510 real sec
sorting block 69, #reads = 1097930 mem = 536878990 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309446, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (264.33, 137.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732356 reads in 76.073 CPU sec, 2.533 real sec
sorting block 70, #reads = 1097910 mem = 536878387 peak = 936820736
[M::process] read 731778 sequences (100000183 bp) in 3.483 CPU sec, 3.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308913, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 157, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (184.18, 66.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (265.83, 137.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731778 reads in 77.049 CPU sec, 2.504 real sec
[M::process] read 731514 sequences (100000077 bp) in 2.937 CPU sec, 3.341 real sec...
sorting block 71, #reads = 1097097 mem = 536878671 peak = 936820736
[M::process] read 731616 sequences (100000266 bp) in 1.774 CPU sec, 1.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 308799, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 129, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (190.75, 126.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (265.25, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732392 sequences (100000052 bp) in 1.269 CPU sec, 1.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309236, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (265.42, 136.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732486 sequences (100000032 bp) in 1.240 CPU sec, 1.246 real sec...
[M::mem_process_seqs] Processed 731514 reads in 142.789 CPU sec, 4.500 real sec
[M::mem_process_seqs] Processed 731616 reads in 79.150 CPU sec, 2.508 real sec
sorting block 72, #reads = 1096687 mem = 536877918 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309417, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 179, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417)
[M::mem_pestat] mean and std.dev: (163.50, 57.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (262.98, 135.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732064 sequences (100000184 bp) in 2.191 CPU sec, 2.389 real sec...
[M::process] read 732294 sequences (100000281 bp) in 1.300 CPU sec, 1.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309664, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (261.81, 134.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732392 reads in 140.203 CPU sec, 4.464 real sec
sorting block 73, #reads = 1096728 mem = 536879089 peak = 936820736
[M::process] read 732048 sequences (100000207 bp) in 1.156 CPU sec, 1.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309434, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (260.25, 132.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732064 reads in 77.447 CPU sec, 2.619 real sec
sorting block 74, #reads = 1097177 mem = 536880697 peak = 936820736
[M::mem_process_seqs] Processed 732486 reads in 173.644 CPU sec, 5.678 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309498, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 235, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (259.56, 132.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 217, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (209.82, 99.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RR
[M::process] read 732148 sequences (100000193 bp) in 1.639 CPU sec, 1.724 real sec...
[M::process] read 732490 sequences (100000170 bp) in 1.230 CPU sec, 1.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 309254, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 237, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (261.88, 133.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 201, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (185.33, 52.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732294 reads in 148.027 CPU sec, 4.731 real sec
[M::mem_process_seqs] Processed 732048 reads in 78.849 CPU sec, 2.520 real sec
sorting block 75, #reads = 1097063 mem = 536879694 peak = 936820736
sorting block 76, #reads = 1096981 mem = 536878308 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309054, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (261.75, 133.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731984 sequences (100000241 bp) in 1.897 CPU sec, 2.006 real sec...
[M::process] read 732030 sequences (100000151 bp) in 1.250 CPU sec, 1.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309721, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (261.36, 134.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732148 reads in 145.690 CPU sec, 4.651 real sec
[M::mem_process_seqs] Processed 732490 reads in 81.780 CPU sec, 2.616 real sec
sorting block 77, #reads = 1096938 mem = 536879257 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309175, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 148, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443)
[M::mem_pestat] mean and std.dev: (256.27, 197.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1878)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (262.27, 134.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731624 sequences (100000075 bp) in 2.231 CPU sec, 2.344 real sec...
[M::process] read 731848 sequences (100000110 bp) in 1.260 CPU sec, 1.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309458, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 144, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429)
[M::mem_pestat] mean and std.dev: (133.75, 37.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 534)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (262.19, 134.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731984 reads in 143.949 CPU sec, 4.588 real sec
sorting block 78, #reads = 1097081 mem = 536881961 peak = 936820736
[M::process] read 731726 sequences (100000050 bp) in 1.204 CPU sec, 1.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 309463, 12, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 181, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437)
[M::mem_pestat] mean and std.dev: (184.83, 60.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 535)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (263.64, 134.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 67, 84)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 128)
[M::mem_pestat] mean and std.dev: (72.00, 15.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 150)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 731624 reads in 77.120 CPU sec, 2.584 real sec
[M::mem_process_seqs] Processed 732030 reads in 174.760 CPU sec, 5.697 real sec
sorting block 79, #reads = 1095918 mem = 536879339 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309607, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (263.23, 134.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 192, 1043)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2823)
[M::mem_pestat] mean and std.dev: (495.73, 568.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3713)
[M::mem_pestat] skip orientation RR
[M::process] read 731014 sequences (100000156 bp) in 1.838 CPU sec, 1.958 real sec...
[M::process] read 730318 sequences (100000212 bp) in 1.210 CPU sec, 1.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309074, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.62, 134.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731848 reads in 151.197 CPU sec, 4.826 real sec
[M::mem_process_seqs] Processed 731726 reads in 81.005 CPU sec, 2.587 real sec
sorting block 80, #reads = 1096843 mem = 536878357 peak = 936820736
[M::process] read 730302 sequences (100000204 bp) in 1.952 CPU sec, 2.109 real sec...
[M::process] read 730344 sequences (100000203 bp) in 1.630 CPU sec, 1.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308513, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (267.93, 136.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730318 reads in 81.104 CPU sec, 2.573 real sec
sorting block 81, #reads = 1096261 mem = 536877010 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308683, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (265.77, 135.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308322, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 159, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354)
[M::mem_pestat] mean and std.dev: (152.64, 40.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.90, 136.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728362 sequences (100000004 bp) in 1.230 CPU sec, 1.231 real sec...
[M::mem_process_seqs] Processed 731014 reads in 227.440 CPU sec, 7.244 real sec
[M::mem_process_seqs] Processed 730302 reads in 89.834 CPU sec, 2.895 real sec
sorting block 82, #reads = 1095857 mem = 536880796 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308983, 9, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.73, 137.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 218, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (223.64, 93.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] skip orientation RR
[M::process] read 732988 sequences (100000107 bp) in 2.489 CPU sec, 2.634 real sec...
[M::process] read 733870 sequences (100000257 bp) in 1.290 CPU sec, 1.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307551, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 198, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (199.64, 62.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 581)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.57, 141.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730344 reads in 148.957 CPU sec, 4.743 real sec
[M::mem_process_seqs] Processed 728362 reads in 83.645 CPU sec, 2.657 real sec
sorting block 83, #reads = 1095159 mem = 536880247 peak = 936820736
sorting block 84, #reads = 1094776 mem = 536877548 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 309312, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 138, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461)
[M::mem_pestat] mean and std.dev: (155.58, 50.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (262.38, 136.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732890 sequences (100000243 bp) in 1.976 CPU sec, 2.103 real sec...
[M::process] read 732778 sequences (100000096 bp) in 1.229 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 310120, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 176, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (205.44, 108.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 235, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (261.23, 135.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732988 reads in 150.770 CPU sec, 4.812 real sec
[M::mem_process_seqs] Processed 733870 reads in 83.814 CPU sec, 2.673 real sec
sorting block 85, #reads = 1096932 mem = 536876753 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309668, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 732600 sequences (100000133 bp) in 1.851 CPU sec, 1.981 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (262.29, 135.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732310 sequences (100000201 bp) in 1.210 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309531, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 151, 166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (160.90, 53.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 376)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (263.43, 136.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732890 reads in 152.229 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 732778 reads in 84.425 CPU sec, 2.709 real sec
sorting block 86, #reads = 1097644 mem = 536880067 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309608, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (263.66, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 271, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (244.80, 98.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RR
[M::process] read 731206 sequences (100000223 bp) in 2.083 CPU sec, 2.180 real sec...
[M::process] read 731318 sequences (100000083 bp) in 1.730 CPU sec, 1.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309443, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (264.32, 136.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 172, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (174.36, 63.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 522)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732600 reads in 154.614 CPU sec, 4.912 real sec
[M::mem_process_seqs] Processed 732310 reads in 84.971 CPU sec, 2.694 real sec
sorting block 87, #reads = 1097592 mem = 536877337 peak = 936820736
sorting block 88, #reads = 1097256 mem = 536878051 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308980, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (265.99, 136.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731444 sequences (100000161 bp) in 2.549 CPU sec, 2.679 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308770, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 168, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (196.08, 61.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.25, 137.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730816 sequences (100000201 bp) in 1.730 CPU sec, 1.732 real sec...
[M::mem_process_seqs] Processed 731206 reads in 153.557 CPU sec, 4.868 real sec
[M::mem_process_seqs] Processed 731318 reads in 85.302 CPU sec, 2.705 real sec
sorting block 89, #reads = 1096517 mem = 536878496 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 309042, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 200, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580)
[M::mem_pestat] mean and std.dev: (198.60, 78.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (266.95, 137.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730626 sequences (100000050 bp) in 2.159 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308237, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 214, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (223.22, 58.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 638)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (268.18, 137.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730806 sequences (100000130 bp) in 1.710 CPU sec, 1.711 real sec...
[M::mem_process_seqs] Processed 731444 reads in 152.013 CPU sec, 4.832 real sec
[M::mem_process_seqs] Processed 730816 reads in 84.411 CPU sec, 2.667 real sec
sorting block 90, #reads = 1096347 mem = 536876885 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309089, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 158, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (135.75, 34.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.21, 135.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730782 sequences (100000284 bp) in 2.349 CPU sec, 2.465 real sec...
[M::process] read 730854 sequences (100000015 bp) in 1.350 CPU sec, 1.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308766, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.11, 136.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730626 reads in 152.311 CPU sec, 4.844 real sec
[M::mem_process_seqs] Processed 730806 reads in 84.823 CPU sec, 2.694 real sec
sorting block 91, #reads = 1095875 mem = 536879935 peak = 936820736
sorting block 92, #reads = 1096091 mem = 536879153 peak = 936820736
[M::process] read 730956 sequences (100000070 bp) in 1.805 CPU sec, 1.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308343, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 144, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 324)
[M::mem_pestat] mean and std.dev: (170.62, 37.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 384)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.95, 137.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730634 sequences (100000119 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308844, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 214, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (194.82, 48.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.93, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730782 reads in 156.346 CPU sec, 4.990 real sec
[M::mem_process_seqs] Processed 730854 reads in 87.274 CPU sec, 2.793 real sec
sorting block 93, #reads = 1095833 mem = 536879648 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308885, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 191, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (202.38, 68.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.59, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 215, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 369)
[M::mem_pestat] mean and std.dev: (209.73, 49.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 431)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730768 sequences (100000111 bp) in 2.278 CPU sec, 2.384 real sec...
[M::process] read 730342 sequences (100000127 bp) in 1.740 CPU sec, 1.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307839, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.58, 137.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730956 reads in 157.764 CPU sec, 5.010 real sec
[M::mem_process_seqs] Processed 730634 reads in 88.135 CPU sec, 2.786 real sec
sorting block 94, #reads = 1096023 mem = 536878159 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308523, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.56, 137.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730548 sequences (100000042 bp) in 2.116 CPU sec, 2.253 real sec...
[M::process] read 730804 sequences (100000196 bp) in 1.700 CPU sec, 1.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308640, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (269.07, 138.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 180, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (184.30, 49.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 521)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730768 reads in 156.763 CPU sec, 4.985 real sec
[M::mem_process_seqs] Processed 730342 reads in 85.941 CPU sec, 2.720 real sec
sorting block 95, #reads = 1095931 mem = 536877777 peak = 936820736
sorting block 96, #reads = 1095352 mem = 536878706 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308306, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 181, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (169.54, 37.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.66, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730518 sequences (100000044 bp) in 2.172 CPU sec, 2.289 real sec...
[M::process] read 729802 sequences (100000146 bp) in 1.240 CPU sec, 1.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308484, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.89, 137.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 176, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (228.10, 88.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730548 reads in 155.761 CPU sec, 4.973 real sec
[M::mem_process_seqs] Processed 730804 reads in 86.181 CPU sec, 2.740 real sec
sorting block 97, #reads = 1095576 mem = 536879662 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308504, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (269.53, 138.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730302 sequences (100000023 bp) in 1.834 CPU sec, 1.940 real sec...
[M::process] read 731106 sequences (100000149 bp) in 1.230 CPU sec, 1.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 307755, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 146, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 265)
[M::mem_pestat] mean and std.dev: (147.67, 26.59)
[M::mem_pestat] low and high boundaries for proper pairs: (7, 308)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (272.30, 140.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730518 reads in 156.447 CPU sec, 4.994 real sec
[M::mem_process_seqs] Processed 729802 reads in 86.200 CPU sec, 2.755 real sec
sorting block 98, #reads = 1095563 mem = 536879517 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308196, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (269.76, 139.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731198 sequences (100000224 bp) in 2.455 CPU sec, 2.576 real sec...
[M::process] read 735130 sequences (100000117 bp) in 1.770 CPU sec, 1.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308818, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.61, 136.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730302 reads in 158.190 CPU sec, 5.016 real sec
sorting block 99, #reads = 1094990 mem = 536879059 peak = 936820736
[M::mem_process_seqs] Processed 731106 reads in 88.668 CPU sec, 2.802 real sec
sorting block 100, #reads = 1095560 mem = 536878718 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308898, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (266.84, 138.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 117, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (147.55, 64.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 558)
[M::mem_pestat] skip orientation RR
[M::process] read 733180 sequences (100000221 bp) in 2.126 CPU sec, 2.235 real sec...
[M::process] read 732588 sequences (100000026 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 311027, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 230, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (256.11, 133.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731198 reads in 158.452 CPU sec, 5.047 real sec
[M::mem_process_seqs] Processed 735130 reads in 88.024 CPU sec, 2.800 real sec
sorting block 101, #reads = 1096046 mem = 536877835 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309755, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 235, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (260.42, 134.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 184, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (209.44, 111.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] skip orientation RR
[M::process] read 732308 sequences (100000184 bp) in 1.711 CPU sec, 1.835 real sec...
[M::process] read 732062 sequences (100000248 bp) in 1.220 CPU sec, 1.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 309273, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (262.86, 135.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733180 reads in 152.562 CPU sec, 4.883 real sec
[M::mem_process_seqs] Processed 732588 reads in 85.953 CPU sec, 2.743 real sec
sorting block 102, #reads = 1098766 mem = 536879834 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309010, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 165, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434)
[M::mem_pestat] mean and std.dev: (191.30, 68.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (263.78, 136.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 177, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432)
[M::mem_pestat] mean and std.dev: (172.40, 51.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 526)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 731694 sequences (100000054 bp) in 2.103 CPU sec, 2.215 real sec...
[M::process] read 731314 sequences (100000165 bp) in 1.230 CPU sec, 1.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308693, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (264.02, 136.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732308 reads in 160.275 CPU sec, 5.108 real sec
[M::mem_process_seqs] Processed 732062 reads in 86.989 CPU sec, 2.777 real sec
sorting block 103, #reads = 1097383 mem = 536877763 peak = 936820736
sorting block 104, #reads = 1095124 mem = 536878660 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309226, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (265.61, 136.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731248 sequences (100000118 bp) in 2.451 CPU sec, 2.571 real sec...
[M::process] read 730894 sequences (100000224 bp) in 1.730 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309240, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.15, 137.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731694 reads in 157.305 CPU sec, 4.991 real sec
[M::mem_process_seqs] Processed 731314 reads in 86.113 CPU sec, 2.721 real sec
sorting block 105, #reads = 1096708 mem = 536881176 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308923, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 275, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653)
[M::mem_pestat] mean and std.dev: (365.62, 289.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2160)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.11, 137.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 225, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (208.00, 77.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730502 sequences (100000017 bp) in 1.714 CPU sec, 1.817 real sec...
[M::process] read 730588 sequences (100000055 bp) in 1.210 CPU sec, 1.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308846, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 197, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426)
[M::mem_pestat] mean and std.dev: (180.45, 50.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.12, 137.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 213, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (208.00, 68.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731248 reads in 156.378 CPU sec, 5.003 real sec
[M::mem_process_seqs] Processed 730894 reads in 87.353 CPU sec, 2.797 real sec
sorting block 106, #reads = 1095954 mem = 536878708 peak = 936820736
[M::process] read 730548 sequences (100000215 bp) in 1.821 CPU sec, 1.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308571, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (269.16, 138.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 167, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (175.67, 47.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 609)
[M::mem_pestat] skip orientation RR
[M::process] read 729610 sequences (100000067 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308447, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.50, 136.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 153, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (151.18, 38.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 473)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308307, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (269.20, 138.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (105, 139, 160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270)
[M::mem_pestat] mean and std.dev: (154.60, 60.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730502 reads in 232.909 CPU sec, 7.462 real sec
sorting block 107, #reads = 1096109 mem = 536877386 peak = 936820736
[M::mem_process_seqs] Processed 730588 reads in 160.682 CPU sec, 5.165 real sec
[M::mem_process_seqs] Processed 730548 reads in 167.595 CPU sec, 5.408 real sec
sorting block 108, #reads = 1095642 mem = 536877511 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308017, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.54, 137.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729976 sequences (100000224 bp) in 2.535 CPU sec, 2.819 real sec...
[M::mem_process_seqs] Processed 729610 reads in 87.122 CPU sec, 2.863 real sec
sorting block 109, #reads = 1095601 mem = 536880089 peak = 936820736
[M::process] read 730038 sequences (100000164 bp) in 2.171 CPU sec, 2.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308302, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (270.79, 139.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729660 sequences (100000199 bp) in 1.300 CPU sec, 1.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308600, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (270.33, 138.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 184, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (176.75, 83.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] skip orientation RR
[M::process] read 728538 sequences (100000130 bp) in 1.220 CPU sec, 1.224 real sec...
[M::mem_process_seqs] Processed 729976 reads in 154.977 CPU sec, 4.900 real sec
[M::mem_process_seqs] Processed 730038 reads in 85.436 CPU sec, 2.693 real sec
sorting block 110, #reads = 1094976 mem = 536880067 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308196, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 230, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (58, 423)
[M::mem_pestat] mean and std.dev: (205.40, 53.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (272.23, 139.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 175, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (201.82, 58.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 583)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728434 sequences (100000040 bp) in 2.525 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307604, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 267, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (220.20, 70.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (275.02, 140.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 155, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 328)
[M::mem_pestat] mean and std.dev: (162.13, 43.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 388)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729660 reads in 148.575 CPU sec, 4.724 real sec
sorting block 111, #reads = 1095137 mem = 536878513 peak = 936820736
[M::mem_process_seqs] Processed 728538 reads in 83.821 CPU sec, 2.645 real sec
[M::process] read 728998 sequences (100000144 bp) in 1.558 CPU sec, 1.575 real sec...
sorting block 112, #reads = 1094772 mem = 536878297 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307576, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 170, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (173.50, 59.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (276.43, 141.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729190 sequences (100000119 bp) in 1.864 CPU sec, 1.969 real sec...
[M::process] read 728734 sequences (100000011 bp) in 1.320 CPU sec, 1.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307644, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (274.92, 141.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (212.43, 101.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728434 reads in 146.398 CPU sec, 4.677 real sec
[M::mem_process_seqs] Processed 728998 reads in 82.355 CPU sec, 2.622 real sec
sorting block 113, #reads = 1094159 mem = 536879114 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307755, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (274.83, 141.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728808 sequences (100000050 bp) in 2.286 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307330, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (276.27, 142.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729190 reads in 147.284 CPU sec, 4.693 real sec
[M::mem_process_seqs] Processed 728734 reads in 82.860 CPU sec, 2.619 real sec
[M::process] read 729920 sequences (100000039 bp) in 1.829 CPU sec, 1.872 real sec...
sorting block 114, #reads = 1094450 mem = 536877677 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307521, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 196, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (185.78, 46.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (276.03, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730996 sequences (100000261 bp) in 2.523 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307653, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (271.54, 140.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 343, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (242.50, 89.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728808 reads in 145.403 CPU sec, 4.624 real sec
sorting block 115, #reads = 1093961 mem = 536877258 peak = 936820736
[M::mem_process_seqs] Processed 729920 reads in 82.021 CPU sec, 2.585 real sec
[M::process] read 732110 sequences (100000064 bp) in 2.133 CPU sec, 2.210 real sec...
sorting block 116, #reads = 1094189 mem = 536878599 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308878, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.44, 138.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 734324 sequences (100000247 bp) in 2.535 CPU sec, 2.663 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309249, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 163, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (161.36, 65.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (265.75, 139.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730996 reads in 146.568 CPU sec, 4.674 real sec
[M::mem_process_seqs] Processed 732110 reads in 82.295 CPU sec, 2.598 real sec
sorting block 117, #reads = 1095641 mem = 536878333 peak = 936820736
[M::process] read 732760 sequences (100000179 bp) in 2.438 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 310507, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 232, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (257.90, 134.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732558 sequences (100000074 bp) in 2.269 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 310420, 12, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (261.62, 134.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 84, 9598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28750)
[M::mem_pestat] mean and std.dev: (2468.92, 4117.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38326)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 734324 reads in 143.095 CPU sec, 4.559 real sec
[M::mem_process_seqs] Processed 732760 reads in 81.396 CPU sec, 2.571 real sec
sorting block 118, #reads = 1097518 mem = 536877234 peak = 936820736
[M::process] read 731906 sequences (100000011 bp) in 2.411 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309050, 9, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 195, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (163.50, 59.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 526)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (263.67, 136.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 119, #reads = 1098356 mem = 536879704 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 309276, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 201, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 385)
[M::mem_pestat] mean and std.dev: (206.14, 66.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (264.35, 135.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731458 sequences (100000003 bp) in 2.627 CPU sec, 2.751 real sec...
[M::mem_process_seqs] Processed 732558 reads in 140.149 CPU sec, 4.487 real sec
[M::mem_process_seqs] Processed 731906 reads in 77.472 CPU sec, 2.467 real sec
[M::process] read 731408 sequences (100000273 bp) in 2.360 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308677, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (266.04, 136.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 207, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360)
[M::mem_pestat] mean and std.dev: (150.88, 52.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 433)
[M::mem_pestat] skip orientation RR
sorting block 120, #reads = 1097035 mem = 536879155 peak = 936820736
[M::process] read 731190 sequences (100000059 bp) in 1.869 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309156, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 155, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (207.60, 124.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1386)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (266.68, 137.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731458 reads in 146.189 CPU sec, 4.680 real sec
[M::mem_process_seqs] Processed 731408 reads in 83.369 CPU sec, 2.644 real sec
sorting block 121, #reads = 1095499 mem = 536879726 peak = 936820736
[M::process] read 730646 sequences (100000169 bp) in 2.017 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309061, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.73, 137.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730338 sequences (100000231 bp) in 2.087 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 308433, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.84, 137.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731190 reads in 143.528 CPU sec, 4.581 real sec
[M::mem_process_seqs] Processed 730646 reads in 81.130 CPU sec, 2.565 real sec
[M::process] read 729950 sequences (100000236 bp) in 1.611 CPU sec, 1.669 real sec...
sorting block 122, #reads = 1096568 mem = 536881779 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308301, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (269.21, 138.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 123, #reads = 1095553 mem = 536878267 peak = 936820736
[M::process] read 729774 sequences (100000090 bp) in 2.527 CPU sec, 2.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308239, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (269.71, 137.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 182, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (182.70, 59.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730338 reads in 140.501 CPU sec, 4.483 real sec
[M::mem_process_seqs] Processed 729950 reads in 79.805 CPU sec, 2.528 real sec
[M::process] read 729832 sequences (100000128 bp) in 2.242 CPU sec, 2.422 real sec...
sorting block 124, #reads = 1095536 mem = 536879131 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308405, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.64, 136.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729610 sequences (100000091 bp) in 1.943 CPU sec, 2.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308208, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 178, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (159.62, 50.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (270.73, 138.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 265, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (232.80, 67.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729774 reads in 142.822 CPU sec, 4.560 real sec
[M::mem_process_seqs] Processed 729832 reads in 80.298 CPU sec, 2.542 real sec
[M::process] read 729150 sequences (100000041 bp) in 1.505 CPU sec, 1.555 real sec...
sorting block 125, #reads = 1095174 mem = 536880601 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308143, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 173, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423)
[M::mem_pestat] mean and std.dev: (174.80, 56.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.71, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 293, 4288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12444)
[M::mem_pestat] mean and std.dev: (1856.40, 1985.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16522)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729132 sequences (100000104 bp) in 2.655 CPU sec, 2.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307786, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 164, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 331)
[M::mem_pestat] mean and std.dev: (179.00, 51.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (272.20, 138.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729610 reads in 140.353 CPU sec, 4.476 real sec
[M::mem_process_seqs] Processed 729150 reads in 79.266 CPU sec, 2.506 real sec
sorting block 126, #reads = 1094679 mem = 536884306 peak = 936820736
[M::process] read 728298 sequences (100000203 bp) in 2.500 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307437, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 184, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 363)
[M::mem_pestat] mean and std.dev: (193.40, 58.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.35, 137.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 127, #reads = 1094956 mem = 536878502 peak = 936820736
[M::process] read 727904 sequences (100000098 bp) in 2.391 CPU sec, 2.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307313, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 250, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.30, 138.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729132 reads in 140.381 CPU sec, 4.479 real sec
[M::mem_process_seqs] Processed 728298 reads in 79.458 CPU sec, 2.517 real sec
[M::process] read 727614 sequences (100000034 bp) in 2.301 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307274, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (275.24, 138.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 263, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 589)
[M::mem_pestat] mean and std.dev: (268.82, 100.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RR
sorting block 128, #reads = 1094389 mem = 536879894 peak = 936820736
[M::process] read 726952 sequences (100000052 bp) in 1.904 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307051, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 203, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (30, 360)
[M::mem_pestat] mean and std.dev: (186.50, 41.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (276.29, 139.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727904 reads in 139.667 CPU sec, 4.466 real sec
[M::mem_process_seqs] Processed 727614 reads in 78.933 CPU sec, 2.499 real sec
[M::process] read 727518 sequences (100000152 bp) in 1.944 CPU sec, 2.087 real sec...
sorting block 129, #reads = 1093686 mem = 536878189 peak = 936820736
[M::process] read 727478 sequences (100000229 bp) in 2.099 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306544, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 231, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471)
[M::mem_pestat] mean and std.dev: (175.22, 57.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (276.23, 139.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727518 reads in 74.627 CPU sec, 2.362 real sec
sorting block 130, #reads = 1093160 mem = 536877116 peak = 936820736
[M::process] read 727112 sequences (100000126 bp) in 1.947 CPU sec, 2.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306855, 8, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 140, 150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 198)
[M::mem_pestat] mean and std.dev: (128.33, 19.32)
[M::mem_pestat] low and high boundaries for proper pairs: (51, 222)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (278.09, 139.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 181, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (42, 327)
[M::mem_pestat] mean and std.dev: (172.91, 41.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 384)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307178, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (277.60, 140.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726952 reads in 213.411 CPU sec, 6.812 real sec
[M::mem_process_seqs] Processed 727478 reads in 85.282 CPU sec, 2.719 real sec
sorting block 131, #reads = 1093250 mem = 536878501 peak = 936820736
[M::process] read 728224 sequences (100000228 bp) in 2.124 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306340, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 167, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (177.67, 67.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.02, 140.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730088 sequences (100000018 bp) in 2.421 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307327, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (275.27, 139.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 727112 reads in 138.156 CPU sec, 4.413 real sec
[M::mem_process_seqs] Processed 728224 reads in 78.056 CPU sec, 2.472 real sec
sorting block 132, #reads = 1092599 mem = 536879099 peak = 936820736
[M::process] read 730332 sequences (100000110 bp) in 2.286 CPU sec, 2.436 real sec...
[M::process] read 20296 sequences (2779451 bp) in 0.312 CPU sec, 0.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308277, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.21, 136.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 133, #reads = 1092846 mem = 536879871 peak = 936820736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308311, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (269.12, 138.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730088 reads in 134.205 CPU sec, 4.345 real sec
[M::mem_process_seqs] Processed 730332 reads in 74.545 CPU sec, 2.422 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 8535, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.04, 136.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 20296 reads in 2.110 CPU sec, 0.074 real sec
sorting block 134, #reads = 1094816 mem = 536877060 peak = 936820736
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 147545540
overall: 69579161600 mem 17376.738 user 1070.902 sys 633.716 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.1_25PercentHG514.L004\tPU:L004\tSM:1_25PercentHG514\tLB:1_25PercentHG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1_25PercentHG514_R1.fastq.gz 0004.1_25PercentHG514_R2.fastq.gz
[main] Real time: 633.754 sec; CPU: 18447.643 sec
sorting block 135, #reads = 494888 mem = 242664545 peak = 936820736
uniq header count 1
merging 136 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 6527144405
output reads: 148380197
bam_mem_sort: 136 calls 82.166 user 0.792 sys 83.193 real
bam_write: 136 calls 44.549 user 2.514 sys 47.190 real
execute: 1 calls 31.195 user 1.490 sys 667.824 real
merge_files: 1 calls 31.170 user 1.459 sys 33.673 real
parse_chunk: 2168 calls 88.910 user 24.556 sys 115.276 real
read_chunk: 5876 calls 52.344 user 42.149 sys 515.990 real
sort_block: 1 calls 41.202 user 41.510 sys 497.491 real
write_chunk: 3706 calls 30.779 user 1.128 sys 32.703 real
overall: 2469142528 mem 1437.632 user 91.684 sys 667.848 real
ls: cannot access '*.cram': No such file or directory