cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1_25PercentHG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2003.359 CPU sec, 70.081 real sec
[M::process] read 733628 sequences (100000167 bp) in 1.081 CPU sec, 1.082 real sec...
[M::process] read 732492 sequences (100000035 bp) in 1.729 CPU sec, 1.766 real sec...
[M::process] read 732000 sequences (100000173 bp) in 1.691 CPU sec, 1.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309867, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 201, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (213.08, 83.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (259.35, 132.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731154 sequences (100000234 bp) in 1.633 CPU sec, 1.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309474, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 287, 590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362)
[M::mem_pestat] mean and std.dev: (355.20, 197.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1748)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (262.16, 133.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 190, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 335)
[M::mem_pestat] mean and std.dev: (175.40, 48.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 401)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 733628 reads in 190.734 CPU sec, 6.149 real sec
[M::process] read 731338 sequences (100000000 bp) in 1.412 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309210, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 162, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (181.27, 81.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (264.04, 134.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 329, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (249.47, 106.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732000 reads in 79.639 CPU sec, 2.550 real sec
sorting block 0, #reads = 1097673 mem = 536878219 peak = 619499520
[M::mem_process_seqs] Processed 732492 reads in 201.453 CPU sec, 6.507 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309300, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.11, 135.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730812 sequences (100000209 bp) in 1.745 CPU sec, 2.111 real sec...
sorting block 1, #reads = 1097077 mem = 536879994 peak = 901894144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309090, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 128, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333)
[M::mem_pestat] mean and std.dev: (151.30, 59.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.74, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731184 sequences (100000246 bp) in 1.554 CPU sec, 1.598 real sec...
[M::mem_process_seqs] Processed 731154 reads in 160.678 CPU sec, 5.222 real sec
[M::mem_process_seqs] Processed 731338 reads in 86.941 CPU sec, 2.822 real sec
sorting block 2, #reads = 1096135 mem = 536879075 peak = 901894144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308775, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.18, 135.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730904 sequences (100000240 bp) in 1.728 CPU sec, 1.894 real sec...
[M::process] read 729588 sequences (100000257 bp) in 1.545 CPU sec, 1.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308782, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.15, 135.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730812 reads in 155.087 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 731184 reads in 87.087 CPU sec, 2.821 real sec
sorting block 3, #reads = 1095874 mem = 536879255 peak = 901894144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308619, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 164, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (193.67, 82.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 638)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.46, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729090 sequences (100000170 bp) in 1.751 CPU sec, 1.809 real sec...
[M::process] read 728426 sequences (100000234 bp) in 1.543 CPU sec, 1.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308325, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.10, 136.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730904 reads in 151.434 CPU sec, 4.881 real sec
[M::mem_process_seqs] Processed 729588 reads in 88.379 CPU sec, 2.835 real sec
sorting block 4, #reads = 1096135 mem = 536882091 peak = 912031744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307867, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 288, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (222.30, 70.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.84, 137.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 161, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 268)
[M::mem_pestat] mean and std.dev: (171.22, 34.62)
[M::mem_pestat] low and high boundaries for proper pairs: (33, 310)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728272 sequences (100000136 bp) in 1.517 CPU sec, 1.781 real sec...
sorting block 5, #reads = 1094874 mem = 536880889 peak = 985677824
[M::process] read 728442 sequences (100000238 bp) in 1.340 CPU sec, 1.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307716, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.18, 137.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729090 reads in 153.175 CPU sec, 4.969 real sec
[M::mem_process_seqs] Processed 728426 reads in 87.564 CPU sec, 2.819 real sec
sorting block 6, #reads = 1094299 mem = 536878156 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307559, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (273.37, 137.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 217, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408)
[M::mem_pestat] mean and std.dev: (204.80, 49.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] skip orientation RR
[M::process] read 727712 sequences (100000136 bp) in 1.913 CPU sec, 2.133 real sec...
[M::process] read 728000 sequences (100000204 bp) in 1.523 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308083, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (274.16, 137.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728272 reads in 155.191 CPU sec, 5.058 real sec
[M::mem_process_seqs] Processed 728442 reads in 89.818 CPU sec, 2.927 real sec
sorting block 7, #reads = 1093991 mem = 536878680 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306850, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 164, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396)
[M::mem_pestat] mean and std.dev: (171.88, 53.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 483)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (276.00, 139.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 211, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212)
[M::mem_pestat] mean and std.dev: (318.40, 198.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1555)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727728 sequences (100000023 bp) in 1.641 CPU sec, 2.115 real sec...
[M::process] read 727354 sequences (100000105 bp) in 1.429 CPU sec, 1.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307015, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (275.44, 138.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 727712 reads in 156.191 CPU sec, 5.083 real sec
[M::mem_process_seqs] Processed 728000 reads in 90.466 CPU sec, 2.907 real sec
sorting block 8, #reads = 1093757 mem = 536882812 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 306599, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (277.01, 139.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 165, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (176.30, 66.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RR
sorting block 9, #reads = 1093159 mem = 536878321 peak = 985677824
[M::process] read 727694 sequences (100000068 bp) in 1.642 CPU sec, 2.211 real sec...
[M::process] read 727416 sequences (100000276 bp) in 1.467 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 306985, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (278.43, 141.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 206, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (215.50, 57.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727728 reads in 155.442 CPU sec, 5.092 real sec
[M::mem_process_seqs] Processed 727354 reads in 89.594 CPU sec, 2.865 real sec
sorting block 10, #reads = 1093307 mem = 536880338 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307034, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 155, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363)
[M::mem_pestat] mean and std.dev: (163.17, 44.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 438)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.50, 140.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 727122 sequences (100000165 bp) in 1.767 CPU sec, 2.116 real sec...
[M::process] read 726934 sequences (100000190 bp) in 1.445 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306591, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (277.69, 139.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 727694 reads in 156.055 CPU sec, 5.041 real sec
[M::mem_process_seqs] Processed 727416 reads in 89.765 CPU sec, 2.866 real sec
sorting block 11, #reads = 1092955 mem = 536877046 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306915, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 151, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364)
[M::mem_pestat] mean and std.dev: (166.56, 58.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (278.14, 139.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726596 sequences (100000140 bp) in 1.738 CPU sec, 1.920 real sec...
[M::process] read 726324 sequences (100000130 bp) in 1.515 CPU sec, 1.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306394, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 363)
[M::mem_pestat] mean and std.dev: (185.00, 48.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 431)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (279.29, 140.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727122 reads in 155.600 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 726934 reads in 88.638 CPU sec, 2.858 real sec
sorting block 12, #reads = 1093057 mem = 536880106 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 306851, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (280.26, 140.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 727416 sequences (100000278 bp) in 1.747 CPU sec, 2.079 real sec...
sorting block 13, #reads = 1092494 mem = 536879482 peak = 985677824
[M::process] read 728640 sequences (100000153 bp) in 1.388 CPU sec, 1.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306286, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.11, 140.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726596 reads in 155.071 CPU sec, 5.054 real sec
[M::mem_process_seqs] Processed 726324 reads in 88.947 CPU sec, 2.868 real sec
sorting block 14, #reads = 1092487 mem = 536880254 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307293, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (277.70, 139.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 727306 sequences (100000029 bp) in 1.771 CPU sec, 1.915 real sec...
[M::process] read 733166 sequences (100000128 bp) in 1.507 CPU sec, 1.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307332, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (272.78, 137.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 186, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (227.45, 111.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727416 reads in 155.648 CPU sec, 5.028 real sec
[M::mem_process_seqs] Processed 728640 reads in 89.265 CPU sec, 2.860 real sec
sorting block 15, #reads = 1092266 mem = 536876327 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306623, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (277.17, 139.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 173, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (157.78, 52.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] skip orientation RR
[M::process] read 732164 sequences (100000146 bp) in 1.781 CPU sec, 2.057 real sec...
[M::process] read 731676 sequences (100000199 bp) in 1.469 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309770, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 130, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 240)
[M::mem_pestat] mean and std.dev: (136.10, 39.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 293)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (261.95, 134.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727306 reads in 155.685 CPU sec, 5.052 real sec
[M::mem_process_seqs] Processed 733166 reads in 90.007 CPU sec, 2.912 real sec
sorting block 16, #reads = 1094075 mem = 536881242 peak = 985677824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 309867, 8, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 194, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (203.12, 99.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (263.36, 134.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 17, #reads = 1095150 mem = 536877254 peak = 989577216
[M::process] read 731466 sequences (100000068 bp) in 1.849 CPU sec, 2.003 real sec...
[M::process] read 731708 sequences (100000047 bp) in 1.441 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 309186, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 211, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (186.21, 55.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (265.54, 135.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 283, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (228.67, 100.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732164 reads in 154.913 CPU sec, 5.061 real sec
[M::mem_process_seqs] Processed 731676 reads in 88.458 CPU sec, 2.889 real sec
sorting block 18, #reads = 1097067 mem = 536878738 peak = 989577216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309089, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (266.02, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732298 sequences (100000075 bp) in 1.801 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309342, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (265.42, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 148, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 283)
[M::mem_pestat] mean and std.dev: (148.91, 25.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 335)
[M::mem_pestat] skip orientation RR
[M::process] read 732178 sequences (100000078 bp) in 1.448 CPU sec, 1.670 real sec...
[M::mem_process_seqs] Processed 731466 reads in 153.010 CPU sec, 4.962 real sec
[M::mem_process_seqs] Processed 731708 reads in 88.663 CPU sec, 2.858 real sec
sorting block 19, #reads = 1096480 mem = 536879389 peak = 989577216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309481, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (263.05, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731386 sequences (100000013 bp) in 1.996 CPU sec, 2.251 real sec...
[M::process] read 730348 sequences (100000100 bp) in 1.541 CPU sec, 1.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309427, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 155, 193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329)
[M::mem_pestat] mean and std.dev: (147.00, 28.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 397)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (263.45, 134.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732298 reads in 154.688 CPU sec, 5.023 real sec
[M::mem_process_seqs] Processed 732178 reads in 88.030 CPU sec, 2.893 real sec
sorting block 20, #reads = 1097065 mem = 536879469 peak = 989577216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309382, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (265.99, 136.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1097029 mem = 536879599 peak = 989577216
[M::process] read 728864 sequences (100000152 bp) in 1.812 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308613, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.61, 135.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728452 sequences (100000253 bp) in 1.425 CPU sec, 1.441 real sec...
[M::mem_process_seqs] Processed 731386 reads in 153.325 CPU sec, 5.039 real sec
[M::mem_process_seqs] Processed 730348 reads in 87.670 CPU sec, 2.888 real sec
sorting block 22, #reads = 1096267 mem = 536877098 peak = 989577216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307489, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 210, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (184.56, 45.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (272.00, 137.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728790 sequences (100000204 bp) in 1.808 CPU sec, 1.987 real sec...
[M::process] read 728402 sequences (100000133 bp) in 1.530 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307556, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.83, 137.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728864 reads in 154.236 CPU sec, 4.990 real sec
[M::mem_process_seqs] Processed 728452 reads in 87.307 CPU sec, 2.802 real sec
sorting block 23, #reads = 1094513 mem = 536879564 peak = 989577216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307774, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (272.83, 137.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (54, 90, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (146.60, 179.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 727952 sequences (100000178 bp) in 1.941 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307706, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (275.32, 139.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728278 sequences (100000010 bp) in 1.416 CPU sec, 1.477 real sec...
[M::mem_process_seqs] Processed 728790 reads in 156.157 CPU sec, 5.113 real sec
[M::mem_process_seqs] Processed 728402 reads in 89.604 CPU sec, 2.941 real sec
sorting block 24, #reads = 1094041 mem = 536878030 peak = 989577216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307350, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 173, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (178.00, 72.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (276.65, 140.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 152, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404)
[M::mem_pestat] mean and std.dev: (175.82, 61.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 25, #reads = 1094214 mem = 536883360 peak = 1045749760
[M::process] read 728018 sequences (100000059 bp) in 1.814 CPU sec, 2.117 real sec...
[M::process] read 727996 sequences (100000071 bp) in 1.437 CPU sec, 1.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307916, 8, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 160, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348)
[M::mem_pestat] mean and std.dev: (163.40, 42.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (276.49, 140.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 188, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (180.88, 93.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727952 reads in 155.676 CPU sec, 5.079 real sec
[M::mem_process_seqs] Processed 728278 reads in 89.400 CPU sec, 2.891 real sec
sorting block 26, #reads = 1093819 mem = 536878852 peak = 1045749760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307309, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 281, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (246.22, 78.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (277.42, 140.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 727612 sequences (100000158 bp) in 1.880 CPU sec, 2.200 real sec...
[M::process] read 727618 sequences (100000245 bp) in 1.444 CPU sec, 1.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307554, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 191, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (204.00, 75.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (278.43, 141.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 214, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449)
[M::mem_pestat] mean and std.dev: (186.78, 58.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728018 reads in 153.650 CPU sec, 4.992 real sec
[M::mem_process_seqs] Processed 727996 reads in 88.087 CPU sec, 2.823 real sec
sorting block 27, #reads = 1093678 mem = 536879448 peak = 1045749760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307240, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (278.26, 140.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 163, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (180.20, 79.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 499)
[M::mem_pestat] skip orientation RR
[M::process] read 727162 sequences (100000086 bp) in 1.961 CPU sec, 2.223 real sec...
[M::process] read 726838 sequences (100000231 bp) in 1.551 CPU sec, 1.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307194, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.38, 139.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 235, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (265.10, 126.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727612 reads in 154.638 CPU sec, 5.024 real sec
[M::mem_process_seqs] Processed 727618 reads in 89.222 CPU sec, 2.888 real sec
sorting block 28, #reads = 1093577 mem = 536879579 peak = 1045749760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307269, 4, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.14, 140.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 239, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (234.47, 110.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RR
sorting block 29, #reads = 1093082 mem = 536878962 peak = 1065766912
[M::process] read 726552 sequences (100000196 bp) in 1.744 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306873, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.45, 142.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 726288 sequences (100000029 bp) in 1.390 CPU sec, 1.430 real sec...
[M::mem_process_seqs] Processed 727162 reads in 153.749 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 726838 reads in 88.901 CPU sec, 2.889 real sec
sorting block 30, #reads = 1092802 mem = 536879718 peak = 1065766912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306535, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 202, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (180.40, 63.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.39, 141.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 727250 sequences (100000080 bp) in 1.782 CPU sec, 2.047 real sec...
[M::process] read 728836 sequences (100000151 bp) in 1.457 CPU sec, 1.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306488, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (281.92, 141.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726552 reads in 153.822 CPU sec, 4.990 real sec
[M::mem_process_seqs] Processed 726288 reads in 88.734 CPU sec, 2.842 real sec
sorting block 31, #reads = 1091980 mem = 536877599 peak = 1065766912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307226, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 192, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (213.55, 75.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (277.47, 139.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728782 sequences (100000166 bp) in 1.828 CPU sec, 2.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308020, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.42, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733030 sequences (100000210 bp) in 1.528 CPU sec, 1.575 real sec...
[M::mem_process_seqs] Processed 727250 reads in 155.037 CPU sec, 5.013 real sec
[M::mem_process_seqs] Processed 728836 reads in 89.956 CPU sec, 2.872 real sec
sorting block 32, #reads = 1091545 mem = 536878544 peak = 1065766912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307924, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.37, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732660 sequences (100000235 bp) in 1.520 CPU sec, 1.723 real sec...
sorting block 33, #reads = 1093681 mem = 536876932 peak = 1127161856
[M::process] read 732300 sequences (100000143 bp) in 1.439 CPU sec, 1.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 309544, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (260.89, 133.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 191, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (273.70, 214.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728782 reads in 154.516 CPU sec, 5.020 real sec
[M::mem_process_seqs] Processed 733030 reads in 88.333 CPU sec, 2.846 real sec
sorting block 34, #reads = 1094249 mem = 536881524 peak = 1127161856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309814, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (262.56, 135.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732776 sequences (100000251 bp) in 1.821 CPU sec, 2.131 real sec...
[M::process] read 733388 sequences (100000027 bp) in 1.424 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309655, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (263.94, 135.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (108, 122, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (161.18, 63.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732660 reads in 152.565 CPU sec, 4.929 real sec
[M::mem_process_seqs] Processed 732300 reads in 87.916 CPU sec, 2.837 real sec
sorting block 35, #reads = 1097921 mem = 536879250 peak = 1127161856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 310129, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (262.44, 134.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 182, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (175.70, 42.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation RR
[M::process] read 733404 sequences (100000142 bp) in 1.886 CPU sec, 2.076 real sec...
[M::process] read 733534 sequences (100000129 bp) in 1.519 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 310209, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 202, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (175.09, 61.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 729)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (261.66, 134.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732776 reads in 154.460 CPU sec, 4.987 real sec
[M::mem_process_seqs] Processed 733388 reads in 88.880 CPU sec, 2.904 real sec
sorting block 36, #reads = 1097209 mem = 536877578 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 310312, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 184, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (199.25, 75.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 235, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (258.96, 132.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 37, #reads = 1098027 mem = 536877448 peak = 1135411200
[M::process] read 732504 sequences (100000157 bp) in 1.739 CPU sec, 2.147 real sec...
[M::process] read 731544 sequences (100000048 bp) in 1.372 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 310167, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 235, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (259.21, 133.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733404 reads in 155.293 CPU sec, 5.062 real sec
[M::mem_process_seqs] Processed 733534 reads in 89.802 CPU sec, 2.911 real sec
sorting block 38, #reads = 1097816 mem = 536878535 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309376, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (262.55, 134.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729138 sequences (100000059 bp) in 1.795 CPU sec, 2.011 real sec...
[M::process] read 728346 sequences (100000084 bp) in 1.520 CPU sec, 1.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309507, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (264.99, 135.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 197, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (109, 299)
[M::mem_pestat] mean and std.dev: (193.80, 17.88)
[M::mem_pestat] low and high boundaries for proper pairs: (71, 337)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732504 reads in 156.030 CPU sec, 5.036 real sec
[M::mem_process_seqs] Processed 731544 reads in 89.168 CPU sec, 2.856 real sec
sorting block 39, #reads = 1097784 mem = 536879831 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308085, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.65, 137.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728832 sequences (100000160 bp) in 1.844 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307585, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 168, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495)
[M::mem_pestat] mean and std.dev: (181.60, 118.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.08, 137.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 167, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 300)
[M::mem_pestat] mean and std.dev: (153.75, 36.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 357)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728308 sequences (100000054 bp) in 1.395 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 729138 reads in 155.077 CPU sec, 5.083 real sec
[M::mem_process_seqs] Processed 728346 reads in 89.690 CPU sec, 2.956 real sec
sorting block 40, #reads = 1096666 mem = 536877834 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307950, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 183, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351)
[M::mem_pestat] mean and std.dev: (180.50, 53.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (272.79, 137.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 41, #reads = 1094230 mem = 536876987 peak = 1135411200
[M::process] read 728230 sequences (100000209 bp) in 1.750 CPU sec, 1.907 real sec...
[M::process] read 728216 sequences (100000018 bp) in 1.421 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307510, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (275.68, 139.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728832 reads in 155.936 CPU sec, 5.055 real sec
[M::mem_process_seqs] Processed 728308 reads in 89.639 CPU sec, 2.877 real sec
sorting block 42, #reads = 1094428 mem = 536879687 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307380, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (276.03, 139.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 727782 sequences (100000191 bp) in 1.794 CPU sec, 2.067 real sec...
[M::process] read 727696 sequences (100000222 bp) in 1.382 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307131, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (275.82, 139.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 208, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (238.58, 65.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728230 reads in 151.286 CPU sec, 4.882 real sec
[M::mem_process_seqs] Processed 728216 reads in 88.477 CPU sec, 2.829 real sec
sorting block 43, #reads = 1093898 mem = 536879391 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307261, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (276.90, 140.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 157, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621)
[M::mem_pestat] mean and std.dev: (370.82, 361.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2117)
[M::mem_pestat] skip orientation RR
[M::process] read 726130 sequences (100000008 bp) in 1.966 CPU sec, 2.156 real sec...
[M::process] read 727286 sequences (100000253 bp) in 1.364 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306893, 8, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (278.86, 141.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2074)
[M::mem_pestat] mean and std.dev: (380.15, 300.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2712)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727782 reads in 154.702 CPU sec, 4.979 real sec
sorting block 44, #reads = 1093280 mem = 536878265 peak = 1135411200
[M::mem_process_seqs] Processed 727696 reads in 88.970 CPU sec, 2.836 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306450, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 262, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.66, 141.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1093271 mem = 536878455 peak = 1135411200
[M::process] read 727070 sequences (100000134 bp) in 1.928 CPU sec, 2.184 real sec...
[M::process] read 726350 sequences (100000014 bp) in 1.492 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307042, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 162, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (197.30, 118.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (278.95, 140.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726130 reads in 154.079 CPU sec, 4.960 real sec
[M::mem_process_seqs] Processed 727286 reads in 89.549 CPU sec, 2.851 real sec
sorting block 46, #reads = 1092587 mem = 536881425 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307057, 5, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 290, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (263.00, 110.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.56, 141.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726756 sequences (100000089 bp) in 1.951 CPU sec, 2.174 real sec...
[M::process] read 726506 sequences (100000249 bp) in 1.490 CPU sec, 1.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 306693, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (281.88, 141.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 192, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (174.12, 53.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727070 reads in 152.634 CPU sec, 4.903 real sec
[M::mem_process_seqs] Processed 726350 reads in 87.380 CPU sec, 2.772 real sec
sorting block 47, #reads = 1092738 mem = 536879440 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307023, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (280.96, 141.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 157, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 327)
[M::mem_pestat] mean and std.dev: (161.25, 37.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 389)
[M::mem_pestat] skip orientation RR
[M::process] read 728630 sequences (100000197 bp) in 1.795 CPU sec, 2.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306827, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 728722 sequences (100000111 bp) in 1.454 CPU sec, 1.463 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.56, 141.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726756 reads in 153.380 CPU sec, 4.953 real sec
sorting block 48, #reads = 1092491 mem = 536877000 peak = 1135411200
[M::mem_process_seqs] Processed 726506 reads in 89.298 CPU sec, 2.851 real sec
sorting block 49, #reads = 1092578 mem = 536880792 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308227, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 196, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (108, 298)
[M::mem_pestat] mean and std.dev: (198.62, 17.63)
[M::mem_pestat] low and high boundaries for proper pairs: (70, 336)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (274.36, 139.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 151, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 302)
[M::mem_pestat] mean and std.dev: (160.31, 47.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 360)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728594 sequences (100000262 bp) in 1.971 CPU sec, 2.129 real sec...
[M::process] read 733364 sequences (100000045 bp) in 1.455 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307635, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.65, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728630 reads in 153.539 CPU sec, 4.954 real sec
[M::mem_process_seqs] Processed 728722 reads in 88.083 CPU sec, 2.845 real sec
sorting block 50, #reads = 1093917 mem = 536879980 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307609, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 214, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (334.64, 237.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1527)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (272.76, 137.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 168, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 291)
[M::mem_pestat] mean and std.dev: (150.60, 57.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 379)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 733822 sequences (100000160 bp) in 1.787 CPU sec, 2.083 real sec...
[M::process] read 733700 sequences (100000215 bp) in 1.436 CPU sec, 1.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309747, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (260.35, 133.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 144, 166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (127.91, 33.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 337)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728594 reads in 153.608 CPU sec, 4.954 real sec
[M::mem_process_seqs] Processed 733364 reads in 88.977 CPU sec, 2.831 real sec
sorting block 51, #reads = 1093625 mem = 536878900 peak = 1135411200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 310432, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 236, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (259.28, 132.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732648 sequences (100000020 bp) in 1.831 CPU sec, 2.040 real sec...
[M::process] read 733166 sequences (100000277 bp) in 1.392 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 310052, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (260.12, 133.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733822 reads in 151.724 CPU sec, 4.881 real sec
sorting block 52, #reads = 1096996 mem = 536878201 peak = 1135411200
[M::mem_process_seqs] Processed 733700 reads in 88.048 CPU sec, 2.803 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309445, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (262.63, 134.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1098305 mem = 536878994 peak = 1139875840
[M::process] read 733518 sequences (100000219 bp) in 1.700 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309621, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 156, 166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 194)
[M::mem_pestat] mean and std.dev: (158.00, 13.94)
[M::mem_pestat] low and high boundaries for proper pairs: (102, 214)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (260.32, 133.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732992 sequences (100000195 bp) in 1.374 CPU sec, 1.447 real sec...
[M::mem_process_seqs] Processed 732648 reads in 155.137 CPU sec, 5.047 real sec
[M::mem_process_seqs] Processed 733166 reads in 88.528 CPU sec, 2.846 real sec
sorting block 54, #reads = 1097388 mem = 536880434 peak = 1139875840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 310590, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 235, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (258.80, 132.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732658 sequences (100000268 bp) in 2.004 CPU sec, 2.163 real sec...
[M::process] read 732052 sequences (100000208 bp) in 1.432 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309849, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (259.97, 132.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733518 reads in 154.042 CPU sec, 4.978 real sec
[M::mem_process_seqs] Processed 732992 reads in 88.265 CPU sec, 2.833 real sec
sorting block 55, #reads = 1098010 mem = 536879672 peak = 1139875840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309262, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (261.61, 133.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729876 sequences (100000023 bp) in 1.798 CPU sec, 2.093 real sec...
[M::process] read 728608 sequences (100000158 bp) in 1.432 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309515, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (263.18, 134.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 171, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (194.30, 65.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732658 reads in 153.213 CPU sec, 4.951 real sec
sorting block 56, #reads = 1098236 mem = 536880202 peak = 1140920320
[M::mem_process_seqs] Processed 732052 reads in 87.561 CPU sec, 2.814 real sec
sorting block 57, #reads = 1097427 mem = 536879382 peak = 1140920320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308331, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.14, 135.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728262 sequences (100000008 bp) in 1.977 CPU sec, 2.088 real sec...
[M::process] read 728322 sequences (100000171 bp) in 1.442 CPU sec, 1.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307869, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (272.55, 137.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 235, 1026)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2768)
[M::mem_pestat] mean and std.dev: (645.36, 562.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3639)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729876 reads in 153.101 CPU sec, 4.936 real sec
[M::mem_process_seqs] Processed 728608 reads in 87.628 CPU sec, 2.827 real sec
sorting block 58, #reads = 1095471 mem = 536879789 peak = 1140920320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307612, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (273.35, 137.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 727486 sequences (100000206 bp) in 1.804 CPU sec, 2.036 real sec...
[M::process] read 727692 sequences (100000259 bp) in 1.492 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307168, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (275.69, 139.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728262 reads in 153.733 CPU sec, 4.948 real sec
[M::mem_process_seqs] Processed 728322 reads in 87.876 CPU sec, 2.805 real sec
sorting block 59, #reads = 1094147 mem = 536877729 peak = 1140920320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306844, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 234, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (255.29, 123.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.25, 139.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 241, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 490)
[M::mem_pestat] mean and std.dev: (237.92, 58.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726942 sequences (100000247 bp) in 2.021 CPU sec, 2.236 real sec...
[M::process] read 727432 sequences (100000060 bp) in 1.524 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307012, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (276.95, 139.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 727486 reads in 154.111 CPU sec, 4.949 real sec
sorting block 60, #reads = 1093649 mem = 536881129 peak = 1140957184
[M::mem_process_seqs] Processed 727692 reads in 87.539 CPU sec, 2.786 real sec
sorting block 61, #reads = 1093297 mem = 536879286 peak = 1140957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306554, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (279.33, 140.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 150, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531)
[M::mem_pestat] mean and std.dev: (150.44, 54.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 663)
[M::mem_pestat] skip orientation RR
[M::process] read 727588 sequences (100000181 bp) in 1.752 CPU sec, 2.042 real sec...
[M::process] read 722368 sequences (100000203 bp) in 1.378 CPU sec, 1.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307036, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.82, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 132, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566)
[M::mem_pestat] mean and std.dev: (157.70, 66.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 715)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726942 reads in 154.374 CPU sec, 4.991 real sec
[M::mem_process_seqs] Processed 727432 reads in 88.872 CPU sec, 2.848 real sec
sorting block 62, #reads = 1092747 mem = 536878644 peak = 1140957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307144, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 136, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470)
[M::mem_pestat] mean and std.dev: (151.67, 54.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (278.09, 140.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726632 sequences (100000029 bp) in 1.834 CPU sec, 1.931 real sec...
[M::process] read 726790 sequences (100000224 bp) in 1.491 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305136, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 237, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175)
[M::mem_pestat] mean and std.dev: (384.40, 316.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1652)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (299.63, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727588 reads in 151.459 CPU sec, 4.851 real sec
[M::mem_process_seqs] Processed 722368 reads in 84.833 CPU sec, 2.688 real sec
sorting block 63, #reads = 1093266 mem = 536879302 peak = 1142812672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 306549, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (280.62, 141.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1090070 mem = 536877186 peak = 1149927424
[M::process] read 727030 sequences (100000100 bp) in 1.916 CPU sec, 2.061 real sec...
[M::process] read 727728 sequences (100000063 bp) in 1.525 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306818, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (280.30, 141.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726632 reads in 152.352 CPU sec, 4.887 real sec
[M::mem_process_seqs] Processed 726790 reads in 87.449 CPU sec, 2.795 real sec
sorting block 65, #reads = 1092199 mem = 536880092 peak = 1149927424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306888, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 146, 173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291)
[M::mem_pestat] mean and std.dev: (141.22, 58.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 374)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (279.11, 139.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729114 sequences (100000043 bp) in 2.021 CPU sec, 2.163 real sec...
[M::process] read 727132 sequences (100000247 bp) in 1.558 CPU sec, 1.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307111, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.17, 139.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 275, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (263.78, 97.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727030 reads in 153.717 CPU sec, 4.942 real sec
[M::mem_process_seqs] Processed 727728 reads in 88.058 CPU sec, 2.834 real sec
sorting block 66, #reads = 1092345 mem = 536879524 peak = 1149927424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 307828, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.83, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 238, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (242.33, 94.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RR
[M::process] read 728608 sequences (100000015 bp) in 1.949 CPU sec, 2.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307264, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 177, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (173.89, 71.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (278.19, 139.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 734186 sequences (100000042 bp) in 1.529 CPU sec, 1.537 real sec...
[M::mem_process_seqs] Processed 729114 reads in 152.812 CPU sec, 4.915 real sec
[M::mem_process_seqs] Processed 727132 reads in 87.235 CPU sec, 2.774 real sec
sorting block 67, #reads = 1093291 mem = 536881114 peak = 1149927424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307748, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (272.16, 137.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1093282 mem = 536876902 peak = 1154854912
[M::process] read 732892 sequences (100000057 bp) in 1.673 CPU sec, 1.842 real sec...
[M::process] read 732300 sequences (100000038 bp) in 1.555 CPU sec, 1.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309770, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (259.71, 134.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728608 reads in 156.637 CPU sec, 5.046 real sec
[M::mem_process_seqs] Processed 734186 reads in 90.667 CPU sec, 2.888 real sec
sorting block 69, #reads = 1095744 mem = 536878746 peak = 1154854912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309307, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (262.56, 136.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 211, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (187.42, 72.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 599)
[M::mem_pestat] skip orientation RR
[M::process] read 731920 sequences (100000191 bp) in 1.867 CPU sec, 2.175 real sec...
[M::process] read 731884 sequences (100000044 bp) in 1.533 CPU sec, 1.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309257, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 257, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (215.00, 79.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (265.02, 137.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 209, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (199.58, 77.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732892 reads in 158.492 CPU sec, 5.098 real sec
[M::mem_process_seqs] Processed 732300 reads in 90.652 CPU sec, 2.905 real sec
sorting block 70, #reads = 1097702 mem = 536878297 peak = 1154854912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309492, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 178, 193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 281)
[M::mem_pestat] mean and std.dev: (177.42, 37.26)
[M::mem_pestat] low and high boundaries for proper pairs: (17, 326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (265.31, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731734 sequences (100000007 bp) in 1.983 CPU sec, 2.185 real sec...
[M::process] read 732046 sequences (100000189 bp) in 1.549 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309399, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 175, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (203.75, 84.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (265.32, 136.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731920 reads in 156.546 CPU sec, 5.029 real sec
[M::mem_process_seqs] Processed 731884 reads in 90.172 CPU sec, 2.873 real sec
sorting block 71, #reads = 1096847 mem = 536878128 peak = 1163440128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309227, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (266.35, 137.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 258, 3815)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11099)
[M::mem_pestat] mean and std.dev: (1398.92, 1708.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14741)
[M::mem_pestat] skip orientation RR
sorting block 72, #reads = 1097184 mem = 536880094 peak = 1163440128
[M::process] read 731836 sequences (100000111 bp) in 2.007 CPU sec, 2.157 real sec...
[M::process] read 731102 sequences (100000048 bp) in 1.514 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309144, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (262.64, 135.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731734 reads in 157.236 CPU sec, 5.043 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 309193, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 208, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 392)
[M::mem_pestat] mean and std.dev: (207.67, 69.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (263.05, 134.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::process] read 730856 sequences (100000143 bp) in 1.467 CPU sec, 1.511 real sec...
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731836 reads in 86.532 CPU sec, 2.781 real sec
sorting block 73, #reads = 1096723 mem = 536879622 peak = 1163440128
[M::mem_process_seqs] Processed 732046 reads in 211.205 CPU sec, 6.769 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308590, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 172, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482)
[M::mem_pestat] mean and std.dev: (187.70, 72.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (266.04, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 74, #reads = 1097011 mem = 536884575 peak = 1163440128
[M::process] read 730474 sequences (100000055 bp) in 1.757 CPU sec, 1.978 real sec...
[M::process] read 730576 sequences (100000014 bp) in 1.514 CPU sec, 1.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308580, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 224, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (240.42, 104.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (268.12, 138.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 201, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 301)
[M::mem_pestat] mean and std.dev: (197.67, 21.61)
[M::mem_pestat] low and high boundaries for proper pairs: (55, 342)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731102 reads in 164.815 CPU sec, 5.293 real sec
[M::mem_process_seqs] Processed 730856 reads in 89.702 CPU sec, 2.881 real sec
sorting block 75, #reads = 1096360 mem = 536878364 peak = 1163440128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307945, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (269.15, 138.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729984 sequences (100000229 bp) in 1.896 CPU sec, 2.155 real sec...
[M::process] read 730194 sequences (100000219 bp) in 1.570 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307931, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 188, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 336)
[M::mem_pestat] mean and std.dev: (177.08, 56.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 403)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (269.01, 138.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730474 reads in 157.271 CPU sec, 5.064 real sec
[M::mem_process_seqs] Processed 730576 reads in 89.601 CPU sec, 2.869 real sec
sorting block 76, #reads = 1095211 mem = 536879411 peak = 1163440128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308026, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (270.54, 138.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 297, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319)
[M::mem_pestat] mean and std.dev: (352.56, 280.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1700)
[M::mem_pestat] skip orientation RR
[M::process] read 730002 sequences (100000215 bp) in 1.814 CPU sec, 2.101 real sec...
[M::process] read 729680 sequences (100000264 bp) in 1.555 CPU sec, 1.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308225, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 203, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 425)
[M::mem_pestat] mean and std.dev: (194.60, 36.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 503)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (270.62, 139.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729984 reads in 154.916 CPU sec, 4.972 real sec
[M::mem_process_seqs] Processed 730194 reads in 88.810 CPU sec, 2.818 real sec
sorting block 77, #reads = 1095077 mem = 536879737 peak = 1163440128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308308, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (270.95, 139.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1095397 mem = 536876249 peak = 1165918208
[M::process] read 729358 sequences (100000269 bp) in 1.882 CPU sec, 2.157 real sec...
[M::process] read 729674 sequences (100000051 bp) in 1.584 CPU sec, 1.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307921, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (272.08, 139.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730002 reads in 156.214 CPU sec, 5.023 real sec
[M::mem_process_seqs] Processed 729680 reads in 89.622 CPU sec, 2.851 real sec
sorting block 79, #reads = 1095209 mem = 536876477 peak = 1165918208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307920, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 154, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 369)
[M::mem_pestat] mean and std.dev: (177.73, 54.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (272.24, 139.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728944 sequences (100000049 bp) in 1.973 CPU sec, 2.127 real sec...
[M::process] read 728852 sequences (100000107 bp) in 1.539 CPU sec, 1.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307558, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 248, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (273.12, 140.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729358 reads in 155.084 CPU sec, 4.968 real sec
[M::mem_process_seqs] Processed 729674 reads in 88.956 CPU sec, 2.814 real sec
sorting block 80, #reads = 1095126 mem = 536877218 peak = 1165918208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307989, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 285, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524)
[M::mem_pestat] mean and std.dev: (350.50, 215.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1954)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (273.97, 140.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1485)
[M::mem_pestat] mean and std.dev: (432.50, 344.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1915)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728604 sequences (100000085 bp) in 1.886 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307318, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 728188 sequences (100000229 bp) in 1.606 CPU sec, 1.610 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (274.06, 139.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 270, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (295.80, 119.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728944 reads in 156.477 CPU sec, 5.032 real sec
[M::mem_process_seqs] Processed 728852 reads in 90.302 CPU sec, 2.864 real sec
sorting block 81, #reads = 1094101 mem = 536878820 peak = 1165918208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307671, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 330, 916)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2414)
[M::mem_pestat] mean and std.dev: (546.10, 560.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3163)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (275.81, 141.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 279, 593)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473)
[M::mem_pestat] mean and std.dev: (382.53, 282.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1913)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 82, #reads = 1094413 mem = 536877317 peak = 1169571840
[M::process] read 728448 sequences (100000223 bp) in 1.959 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307190, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 162, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (158.50, 70.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (277.59, 141.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730078 sequences (100000053 bp) in 1.582 CPU sec, 1.587 real sec...
[M::mem_process_seqs] Processed 728604 reads in 156.125 CPU sec, 5.014 real sec
[M::mem_process_seqs] Processed 728188 reads in 90.389 CPU sec, 2.861 real sec
sorting block 83, #reads = 1093824 mem = 536879360 peak = 1169571840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307600, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (275.71, 141.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 309, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (297.83, 70.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RR
[M::process] read 729276 sequences (100000264 bp) in 1.954 CPU sec, 2.159 real sec...
[M::process] read 733818 sequences (100000002 bp) in 1.627 CPU sec, 1.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308379, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.82, 137.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 210, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (215.15, 67.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728448 reads in 157.878 CPU sec, 5.061 real sec
[M::mem_process_seqs] Processed 730078 reads in 90.288 CPU sec, 2.877 real sec
sorting block 84, #reads = 1093233 mem = 536877020 peak = 1169571840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 307603, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (273.45, 140.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732852 sequences (100000168 bp) in 1.971 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 310077, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 236, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (261.46, 135.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 206, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (204.92, 67.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation RR
[M::process] read 732424 sequences (100000263 bp) in 1.623 CPU sec, 1.628 real sec...
[M::mem_process_seqs] Processed 729276 reads in 156.963 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 733818 reads in 90.335 CPU sec, 2.862 real sec
sorting block 85, #reads = 1095010 mem = 536878183 peak = 1169571840
sorting block 86, #reads = 1096098 mem = 536877588 peak = 1178185728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309463, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (262.80, 135.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731982 sequences (100000145 bp) in 1.737 CPU sec, 2.012 real sec...
[M::process] read 732388 sequences (100000014 bp) in 1.586 CPU sec, 1.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309263, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (265.29, 137.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732852 reads in 156.382 CPU sec, 5.034 real sec
[M::mem_process_seqs] Processed 732424 reads in 90.858 CPU sec, 2.891 real sec
sorting block 87, #reads = 1097742 mem = 536878094 peak = 1178185728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309011, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (265.24, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733232 sequences (100000199 bp) in 2.007 CPU sec, 2.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309677, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 239, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (263.66, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733210 sequences (100000170 bp) in 1.568 CPU sec, 1.575 real sec...
[M::mem_process_seqs] Processed 731982 reads in 156.504 CPU sec, 5.047 real sec
[M::mem_process_seqs] Processed 732388 reads in 90.834 CPU sec, 2.908 real sec
sorting block 88, #reads = 1096632 mem = 536880175 peak = 1178185728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 310048, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (260.89, 134.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733004 sequences (100000269 bp) in 1.798 CPU sec, 1.953 real sec...
[M::process] read 731768 sequences (100000092 bp) in 1.629 CPU sec, 1.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309817, 10, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 137, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359)
[M::mem_pestat] mean and std.dev: (147.60, 38.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 438)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (258.92, 133.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 55, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191)
[M::mem_pestat] mean and std.dev: (48.40, 27.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 249)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 733232 reads in 157.099 CPU sec, 5.048 real sec
[M::mem_process_seqs] Processed 733210 reads in 90.636 CPU sec, 2.889 real sec
sorting block 89, #reads = 1097404 mem = 536878402 peak = 1188896768
sorting block 90, #reads = 1098104 mem = 536878384 peak = 1188896768
[M::process] read 730964 sequences (100000252 bp) in 1.916 CPU sec, 2.135 real sec...
[M::process] read 730712 sequences (100000147 bp) in 1.608 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 309624, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 122, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (137.92, 42.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (264.07, 135.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731768 reads in 85.347 CPU sec, 2.697 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309837, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (260.21, 133.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308714, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.86, 137.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730182 sequences (100000171 bp) in 1.579 CPU sec, 1.585 real sec...
[M::mem_process_seqs] Processed 733004 reads in 236.505 CPU sec, 7.572 real sec
[M::mem_process_seqs] Processed 730964 reads in 97.041 CPU sec, 3.109 real sec
sorting block 91, #reads = 1097131 mem = 536878736 peak = 1188896768
sorting block 92, #reads = 1096933 mem = 536878805 peak = 1189539840
[M::process] read 730172 sequences (100000048 bp) in 2.181 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308618, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.68, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730206 sequences (100000055 bp) in 1.585 CPU sec, 1.596 real sec...
[M::mem_process_seqs] Processed 730182 reads in 84.539 CPU sec, 2.669 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308478, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.33, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308493, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 182, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (200.08, 71.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (269.84, 138.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730712 reads in 235.599 CPU sec, 7.583 real sec
[M::process] read 730186 sequences (100000011 bp) in 1.551 CPU sec, 1.618 real sec...
[M::mem_process_seqs] Processed 730172 reads in 96.801 CPU sec, 3.153 real sec
sorting block 93, #reads = 1095262 mem = 536877869 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308053, 8, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 201, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (191.50, 82.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (271.03, 139.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 214, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (202.33, 62.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 94, #reads = 1095440 mem = 536879577 peak = 1189539840
[M::process] read 729706 sequences (100000221 bp) in 2.283 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 308280, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 208, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (194.71, 67.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (272.06, 140.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 185, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 329)
[M::mem_pestat] mean and std.dev: (175.18, 25.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 385)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729662 sequences (100000205 bp) in 1.469 CPU sec, 1.470 real sec...
[M::mem_process_seqs] Processed 730206 reads in 158.131 CPU sec, 5.105 real sec
[M::mem_process_seqs] Processed 730186 reads in 90.376 CPU sec, 2.920 real sec
sorting block 95, #reads = 1095328 mem = 536880604 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308306, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (273.14, 140.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729302 sequences (100000263 bp) in 1.941 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307862, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 248, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (272.72, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729228 sequences (100000104 bp) in 1.578 CPU sec, 1.585 real sec...
[M::mem_process_seqs] Processed 729706 reads in 155.264 CPU sec, 5.065 real sec
[M::mem_process_seqs] Processed 729662 reads in 88.154 CPU sec, 2.874 real sec
sorting block 96, #reads = 1095337 mem = 536877067 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307562, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 184, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (174.18, 45.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 249, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (274.34, 141.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728920 sequences (100000239 bp) in 1.981 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307543, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 243, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (246.70, 112.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 249, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (274.42, 141.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728716 sequences (100000143 bp) in 1.587 CPU sec, 1.594 real sec...
[M::mem_process_seqs] Processed 729302 reads in 156.749 CPU sec, 5.053 real sec
[M::mem_process_seqs] Processed 729228 reads in 89.846 CPU sec, 2.859 real sec
sorting block 97, #reads = 1094540 mem = 536879080 peak = 1189539840
sorting block 98, #reads = 1094647 mem = 536879130 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307774, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (275.83, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728294 sequences (100000217 bp) in 2.012 CPU sec, 2.142 real sec...
[M::process] read 729558 sequences (100000193 bp) in 1.617 CPU sec, 1.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 307306, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (276.46, 142.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 189, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 376)
[M::mem_pestat] mean and std.dev: (174.00, 40.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728920 reads in 156.567 CPU sec, 5.029 real sec
[M::mem_process_seqs] Processed 728716 reads in 90.546 CPU sec, 2.882 real sec
sorting block 99, #reads = 1094463 mem = 536876549 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307235, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (277.14, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730594 sequences (100000141 bp) in 1.791 CPU sec, 1.952 real sec...
[M::process] read 730732 sequences (100000063 bp) in 1.655 CPU sec, 1.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308106, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (272.39, 140.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728294 reads in 156.800 CPU sec, 5.026 real sec
[M::mem_process_seqs] Processed 729558 reads in 90.185 CPU sec, 2.857 real sec
sorting block 100, #reads = 1093889 mem = 536877976 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308460, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.35, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733804 sequences (100000225 bp) in 1.660 CPU sec, 1.856 real sec...
[M::process] read 733566 sequences (100000127 bp) in 1.609 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308987, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.64, 138.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 170, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (200.91, 81.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 529)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730594 reads in 155.394 CPU sec, 5.002 real sec
[M::mem_process_seqs] Processed 730732 reads in 90.029 CPU sec, 2.859 real sec
sorting block 101, #reads = 1094544 mem = 536881567 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 310751, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (260.02, 134.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 228, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (230.75, 113.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RR
sorting block 102, #reads = 1095736 mem = 536878398 peak = 1189539840
[M::process] read 732644 sequences (100000026 bp) in 1.728 CPU sec, 1.888 real sec...
[M::process] read 733376 sequences (100000189 bp) in 1.599 CPU sec, 1.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 310152, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 184, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408)
[M::mem_pestat] mean and std.dev: (200.25, 73.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (261.82, 135.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 205, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (211.00, 55.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 515)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 733804 reads in 154.966 CPU sec, 4.999 real sec
[M::mem_process_seqs] Processed 733566 reads in 88.916 CPU sec, 2.841 real sec
sorting block 103, #reads = 1097710 mem = 536878004 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309162, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (263.48, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 182, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (200.08, 80.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] skip orientation RR
[M::process] read 733840 sequences (100000232 bp) in 2.000 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 310003, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (261.36, 134.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 734580 sequences (100000174 bp) in 1.631 CPU sec, 1.633 real sec...
[M::mem_process_seqs] Processed 732644 reads in 157.289 CPU sec, 5.039 real sec
sorting block 104, #reads = 1097981 mem = 536878904 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 310362, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 156, 187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 279)
[M::mem_pestat] mean and std.dev: (157.55, 40.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 325)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (259.32, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 733840 reads in 83.154 CPU sec, 2.660 real sec
[M::process] read 733794 sequences (100000260 bp) in 1.549 CPU sec, 1.592 real sec...
[M::mem_process_seqs] Processed 733376 reads in 210.549 CPU sec, 6.715 real sec
sorting block 105, #reads = 1098042 mem = 536877687 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 310837, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 232, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (257.30, 132.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 734076 sequences (100000206 bp) in 2.013 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 310331, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 232, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (256.44, 131.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 734580 reads in 163.184 CPU sec, 5.211 real sec
[M::process] read 732966 sequences (100000096 bp) in 1.564 CPU sec, 1.579 real sec...
sorting block 106, #reads = 1098423 mem = 536877908 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 310238, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 233, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (257.26, 132.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731158 sequences (100000035 bp) in 1.711 CPU sec, 1.757 real sec...
[M::mem_process_seqs] Processed 734076 reads in 82.890 CPU sec, 2.648 real sec
[M::mem_process_seqs] Processed 733794 reads in 219.046 CPU sec, 7.017 real sec
sorting block 107, #reads = 1098454 mem = 536879333 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309795, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 189, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (182.44, 47.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (260.52, 134.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (103, 142, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (149.70, 51.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 503)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730302 sequences (100000273 bp) in 2.182 CPU sec, 2.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308861, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 124, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (174.40, 101.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.87, 137.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732966 reads in 164.631 CPU sec, 5.278 real sec
[M::mem_process_seqs] Processed 731158 reads in 91.021 CPU sec, 2.892 real sec
[M::process] read 730238 sequences (100000082 bp) in 1.807 CPU sec, 1.869 real sec...
sorting block 108, #reads = 1098106 mem = 536879952 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308489, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 168, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (204.82, 83.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.67, 136.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 183, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (209.30, 96.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730410 sequences (100000272 bp) in 1.893 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308141, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 222, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (223.70, 76.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (267.66, 136.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730190 sequences (100000116 bp) in 1.619 CPU sec, 1.622 real sec...
[M::mem_process_seqs] Processed 730302 reads in 157.660 CPU sec, 5.056 real sec
sorting block 109, #reads = 1096067 mem = 536885817 peak = 1189539840
[M::mem_process_seqs] Processed 730238 reads in 91.079 CPU sec, 2.887 real sec
sorting block 110, #reads = 1095318 mem = 536878296 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308647, 7, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (269.68, 139.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 159, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 303)
[M::mem_pestat] mean and std.dev: (168.89, 44.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 355)
[M::mem_pestat] skip orientation RR
[M::process] read 730002 sequences (100000015 bp) in 1.719 CPU sec, 1.829 real sec...
[M::process] read 729768 sequences (100000201 bp) in 1.615 CPU sec, 1.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308480, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (270.00, 138.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730410 reads in 156.030 CPU sec, 5.013 real sec
[M::mem_process_seqs] Processed 730190 reads in 89.003 CPU sec, 2.828 real sec
sorting block 111, #reads = 1095930 mem = 536877672 peak = 1189539840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307674, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 217, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (234.53, 82.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (271.15, 139.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729368 sequences (100000231 bp) in 2.043 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308045, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (271.72, 140.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 199, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (189.42, 62.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] skip orientation RR
[M::process] read 729006 sequences (100000075 bp) in 1.539 CPU sec, 1.543 real sec...
[M::mem_process_seqs] Processed 730002 reads in 156.990 CPU sec, 5.042 real sec
[M::mem_process_seqs] Processed 729768 reads in 91.087 CPU sec, 2.889 real sec
sorting block 112, #reads = 1095166 mem = 536881389 peak = 1190105088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308002, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (272.93, 140.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729144 sequences (100000010 bp) in 1.902 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307553, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (276.07, 142.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728758 sequences (100000264 bp) in 1.660 CPU sec, 1.664 real sec...
[M::mem_process_seqs] Processed 729368 reads in 154.893 CPU sec, 4.996 real sec
sorting block 113, #reads = 1094119 mem = 536878016 peak = 1197301760
[M::mem_process_seqs] Processed 729006 reads in 89.085 CPU sec, 2.823 real sec
sorting block 114, #reads = 1094166 mem = 536878395 peak = 1197301760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308105, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 144, 156)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 228)
[M::mem_pestat] mean and std.dev: (140.00, 31.09)
[M::mem_pestat] low and high boundaries for proper pairs: (12, 264)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (274.05, 141.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728520 sequences (100000071 bp) in 1.947 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308026, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (274.55, 140.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728918 sequences (100000225 bp) in 1.618 CPU sec, 1.623 real sec...
[M::mem_process_seqs] Processed 729144 reads in 154.504 CPU sec, 4.988 real sec
[M::mem_process_seqs] Processed 728758 reads in 88.905 CPU sec, 2.867 real sec
sorting block 115, #reads = 1094766 mem = 536878999 peak = 1197301760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307055, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (275.64, 141.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 210, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470)
[M::mem_pestat] mean and std.dev: (187.67, 46.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] skip orientation RR
[M::process] read 730816 sequences (100000059 bp) in 2.055 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307616, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (275.37, 141.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730706 sequences (100000207 bp) in 1.678 CPU sec, 1.686 real sec...
[M::mem_process_seqs] Processed 728520 reads in 155.332 CPU sec, 4.977 real sec
[M::mem_process_seqs] Processed 728918 reads in 89.298 CPU sec, 2.830 real sec
sorting block 116, #reads = 1094065 mem = 536877954 peak = 1197301760
[M::process] read 730542 sequences (100000127 bp) in 2.090 CPU sec, 2.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308933, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 251, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (257.17, 107.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.54, 137.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 734706 sequences (100000276 bp) in 1.645 CPU sec, 1.648 real sec...
[M::mem_process_seqs] Processed 730706 reads in 83.250 CPU sec, 2.622 real sec
sorting block 117, #reads = 1093908 mem = 536879420 peak = 1197301760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 308452, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 152, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 294)
[M::mem_pestat] mean and std.dev: (151.35, 39.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 350)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (268.34, 138.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308439, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.93, 137.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730816 reads in 235.551 CPU sec, 7.554 real sec
[M::process] read 734496 sequences (100000249 bp) in 1.689 CPU sec, 1.704 real sec...
sorting block 118, #reads = 1095474 mem = 536876899 peak = 1197301760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 310768, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 234, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (259.03, 134.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733912 sequences (100000139 bp) in 1.432 CPU sec, 1.444 real sec...
[M::mem_process_seqs] Processed 734706 reads in 84.654 CPU sec, 2.718 real sec
[M::mem_process_seqs] Processed 730542 reads in 216.943 CPU sec, 6.990 real sec
sorting block 119, #reads = 1098144 mem = 536877074 peak = 1197301760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 310421, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (259.14, 133.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733264 sequences (100000229 bp) in 2.326 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 310068, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 194, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340)
[M::mem_pestat] mean and std.dev: (153.90, 41.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 411)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 235, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (259.90, 133.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 734496 reads in 166.426 CPU sec, 5.339 real sec
[M::mem_process_seqs] Processed 733912 reads in 91.739 CPU sec, 2.906 real sec
[M::process] read 733762 sequences (100000098 bp) in 1.843 CPU sec, 1.885 real sec...
sorting block 120, #reads = 1096315 mem = 536881973 peak = 1197301760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 310065, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (260.90, 134.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 145, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416)
[M::mem_pestat] mean and std.dev: (164.82, 55.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RR
sorting block 121, #reads = 1098844 mem = 536881196 peak = 1200996352
[M::process] read 733960 sequences (100000048 bp) in 2.159 CPU sec, 2.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309645, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 235, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (259.32, 133.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733264 reads in 155.766 CPU sec, 4.996 real sec
[M::mem_process_seqs] Processed 733762 reads in 90.757 CPU sec, 2.872 real sec
[M::process] read 733620 sequences (100000287 bp) in 1.672 CPU sec, 1.686 real sec...
sorting block 122, #reads = 1097922 mem = 536880270 peak = 1200996352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 310674, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 233, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (257.45, 132.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733650 sequences (100000193 bp) in 2.041 CPU sec, 2.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309958, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 234, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (257.76, 131.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 733374 sequences (100000138 bp) in 1.636 CPU sec, 1.645 real sec...
[M::mem_process_seqs] Processed 733960 reads in 157.066 CPU sec, 5.047 real sec
[M::mem_process_seqs] Processed 733620 reads in 90.624 CPU sec, 2.875 real sec
sorting block 123, #reads = 1098490 mem = 536879652 peak = 1200996352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 310014, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (257.82, 132.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730372 sequences (100000276 bp) in 2.082 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309950, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 234, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (258.58, 132.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 197, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (233.44, 129.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 733650 reads in 156.694 CPU sec, 5.077 real sec
[M::process] read 729998 sequences (100000013 bp) in 1.630 CPU sec, 1.637 real sec...
[M::mem_process_seqs] Processed 733374 reads in 89.590 CPU sec, 2.887 real sec
sorting block 124, #reads = 1098092 mem = 536876359 peak = 1200996352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308510, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.88, 136.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 125, #reads = 1098363 mem = 536877633 peak = 1200996352
[M::process] read 729780 sequences (100000134 bp) in 2.374 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308539, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 171, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (174.25, 67.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (268.04, 135.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730372 reads in 154.872 CPU sec, 4.965 real sec
[M::process] read 729946 sequences (100000001 bp) in 1.698 CPU sec, 1.715 real sec...
[M::mem_process_seqs] Processed 729998 reads in 89.398 CPU sec, 2.832 real sec
sorting block 126, #reads = 1095556 mem = 536876176 peak = 1200996352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308186, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 194, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 399)
[M::mem_pestat] mean and std.dev: (191.86, 54.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.38, 137.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729204 sequences (100000004 bp) in 2.331 CPU sec, 2.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308065, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (271.09, 139.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729780 reads in 155.846 CPU sec, 5.020 real sec
[M::process] read 729656 sequences (100000014 bp) in 2.071 CPU sec, 2.125 real sec...
sorting block 127, #reads = 1095081 mem = 536879985 peak = 1200996352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307739, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (272.64, 139.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 195, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (162.40, 60.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 607)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729204 reads in 84.843 CPU sec, 2.717 real sec
[M::process] read 729032 sequences (100000076 bp) in 1.931 CPU sec, 1.997 real sec...
[M::mem_process_seqs] Processed 729946 reads in 221.254 CPU sec, 7.053 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307936, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (272.69, 139.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 128, #reads = 1093952 mem = 536878271 peak = 1200996352
[M::process] read 729176 sequences (100000078 bp) in 2.189 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307663, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (272.65, 139.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729656 reads in 163.981 CPU sec, 5.214 real sec
[M::mem_process_seqs] Processed 729032 reads in 90.012 CPU sec, 2.854 real sec
[M::process] read 728056 sequences (100000228 bp) in 1.635 CPU sec, 1.668 real sec...
sorting block 129, #reads = 1094783 mem = 536879298 peak = 1200996352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307729, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (272.74, 139.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 725816 sequences (100000015 bp) in 2.139 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307167, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 166, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334)
[M::mem_pestat] mean and std.dev: (159.36, 31.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (278.19, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729176 reads in 155.323 CPU sec, 4.978 real sec
[M::process] read 728676 sequences (100000041 bp) in 1.666 CPU sec, 1.674 real sec...
[M::mem_process_seqs] Processed 728056 reads in 88.868 CPU sec, 2.817 real sec
sorting block 130, #reads = 1094772 mem = 536879799 peak = 1200996352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 305966, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (289.26, 148.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 131, #reads = 1094387 mem = 536878574 peak = 1202372608
[M::process] read 728820 sequences (100000166 bp) in 2.233 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307141, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (274.72, 139.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 725816 reads in 155.947 CPU sec, 5.008 real sec
[M::mem_process_seqs] Processed 728676 reads in 88.983 CPU sec, 2.808 real sec
[M::process] read 729174 sequences (100000045 bp) in 1.730 CPU sec, 1.750 real sec...
sorting block 132, #reads = 1092379 mem = 536878857 peak = 1202372608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307066, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 165, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420)
[M::mem_pestat] mean and std.dev: (183.18, 55.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (275.13, 140.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731022 sequences (100000017 bp) in 2.155 CPU sec, 2.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308086, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 249, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (273.50, 140.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 187, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 400)
[M::mem_pestat] mean and std.dev: (196.55, 52.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728820 reads in 148.838 CPU sec, 4.744 real sec
[M::process] read 729116 sequences (100000240 bp) in 1.697 CPU sec, 1.705 real sec...
[M::mem_process_seqs] Processed 729174 reads in 86.254 CPU sec, 2.712 real sec
sorting block 133, #reads = 1093774 mem = 536881873 peak = 1202372608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308784, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 222, 657)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1585)
[M::mem_pestat] mean and std.dev: (396.17, 314.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2049)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.62, 137.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 191, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (200.75, 125.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730646 sequences (100000026 bp) in 2.187 CPU sec, 2.339 real sec...
[M::process] read 37012 sequences (5060193 bp) in 0.362 CPU sec, 0.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307797, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (272.55, 139.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 240, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (228.58, 79.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731022 reads in 148.946 CPU sec, 4.791 real sec
[M::mem_process_seqs] Processed 729116 reads in 86.733 CPU sec, 2.801 real sec
sorting block 134, #reads = 1094975 mem = 536879935 peak = 1202372608
sorting block 135, #reads = 1094830 mem = 536876888 peak = 1207480320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308564, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.25, 136.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 15709, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 239, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (265.30, 137.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730646 reads in 84.594 CPU sec, 2.795 real sec
[M::mem_process_seqs] Processed 37012 reads in 12.179 CPU sec, 0.400 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 148972552
overall: 69752705024 mem 18024.860 user 631.637 sys 616.658 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.1_25PercentHG514.L001\tPU:L001\tSM:1_25PercentHG514\tLB:1_25PercentHG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1_25PercentHG514_R1.fastq.gz 0002.1_25PercentHG514_R2.fastq.gz
[main] Real time: 616.686 sec; CPU: 18656.500 sec
sorting block 136, #reads = 881451 mem = 432064537 peak = 1207480320
uniq header count 1
merging 137 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 6533131642
output reads: 149829653
bam_mem_sort: 137 calls 92.103 user 2.145 sys 96.215 real
bam_write: 137 calls 78.204 user 5.300 sys 149.082 real
execute: 1 calls 39.640 user 2.226 sys 661.061 real
merge_files: 1 calls 39.612 user 2.208 sys 43.518 real
parse_chunk: 2189 calls 99.583 user 26.851 sys 152.890 real
read_chunk: 5934 calls 47.653 user 28.111 sys 558.072 real
sort_block: 1 calls 34.086 user 27.231 sys 540.194 real
write_chunk: 3743 calls 39.182 user 1.902 sys 42.433 real
overall: 2719916032 mem 1581.827 user 91.939 sys 661.078 real
ls: cannot access '*.cram': No such file or directory