File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/29/e2571d3610a4fb8192044d79de0fe5/.command.err
Size
190.8 KB
Attempt
This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o SH032_HG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2017.176 CPU sec, 73.393 real sec
[M::process] read 728704 sequences (100000005 bp) in 1.153 CPU sec, 1.154 real sec...
[M::process] read 727268 sequences (100000251 bp) in 1.912 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 307562, 7, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 168, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (165.32, 60.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (274.90, 141.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 164, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (190.38, 78.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726848 sequences (100000165 bp) in 1.910 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306479, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 188, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423)
[M::mem_pestat] mean and std.dev: (182.86, 43.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.64, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 193, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (178.83, 59.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726306 sequences (100000051 bp) in 1.866 CPU sec, 1.946 real sec...
[M::mem_process_seqs] Processed 728704 reads in 185.779 CPU sec, 5.965 real sec
[M::mem_process_seqs] Processed 727268 reads in 106.175 CPU sec, 3.466 real sec
sorting block 0, #reads = 1104583 mem = 536880361 peak = 590352384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306792, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 198, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (189.36, 51.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.61, 143.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 261, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (307.55, 169.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1386)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726542 sequences (100000036 bp) in 2.302 CPU sec, 2.450 real sec...
[M::process] read 725672 sequences (100000105 bp) in 1.754 CPU sec, 1.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306295, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 167, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (179.50, 81.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.85, 144.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 226, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 415)
[M::mem_pestat] mean and std.dev: (218.20, 54.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726848 reads in 186.342 CPU sec, 5.980 real sec
[M::mem_process_seqs] Processed 726306 reads in 107.494 CPU sec, 3.438 real sec
sorting block 1, #reads = 1103672 mem = 536882157 peak = 751968256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306476, 0, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 217, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (334.57, 252.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.66, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 184, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (185.69, 70.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725408 sequences (100000069 bp) in 2.345 CPU sec, 2.553 real sec...
[M::process] read 725468 sequences (100000139 bp) in 1.900 CPU sec, 1.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306364, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 180, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (180.00, 44.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (285.34, 145.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 186, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (185.30, 88.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726542 reads in 185.964 CPU sec, 5.960 real sec
[M::mem_process_seqs] Processed 725672 reads in 107.767 CPU sec, 3.443 real sec
sorting block 2, #reads = 1103369 mem = 536879013 peak = 898437120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306089, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 196, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482)
[M::mem_pestat] mean and std.dev: (218.36, 87.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (286.36, 146.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 188, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (224.20, 97.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725568 sequences (100000157 bp) in 2.223 CPU sec, 2.539 real sec...
[M::process] read 725280 sequences (100000065 bp) in 1.905 CPU sec, 1.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305333, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 179, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377)
[M::mem_pestat] mean and std.dev: (197.76, 74.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.27, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (212.20, 87.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725408 reads in 189.651 CPU sec, 6.098 real sec
sorting block 3, #reads = 1103649 mem = 536879653 peak = 950759424
[M::mem_process_seqs] Processed 725468 reads in 109.206 CPU sec, 3.480 real sec
sorting block 4, #reads = 1102255 mem = 536878193 peak = 994357248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 305796, 1, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (285.62, 146.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 210, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (199.07, 79.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 688)
[M::mem_pestat] skip orientation RR
[M::process] read 724954 sequences (100000013 bp) in 2.130 CPU sec, 2.276 real sec...
[M::process] read 724512 sequences (100000031 bp) in 1.924 CPU sec, 1.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305690, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 249, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (213.55, 73.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (286.36, 145.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 245, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (244.00, 83.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725568 reads in 185.158 CPU sec, 5.964 real sec
[M::mem_process_seqs] Processed 725280 reads in 105.875 CPU sec, 3.391 real sec
sorting block 5, #reads = 1102666 mem = 536878342 peak = 994357248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305607, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 193, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (178.00, 45.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 563)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (288.18, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 203, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (214.00, 112.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 663)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724488 sequences (100000210 bp) in 2.245 CPU sec, 2.424 real sec...
[M::process] read 724364 sequences (100000205 bp) in 1.831 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305461, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 175, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438)
[M::mem_pestat] mean and std.dev: (162.27, 39.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (288.26, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 203, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (221.81, 82.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 591)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724954 reads in 192.005 CPU sec, 6.226 real sec
[M::mem_process_seqs] Processed 724512 reads in 110.216 CPU sec, 3.579 real sec
sorting block 6, #reads = 1102371 mem = 536877768 peak = 994357248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305669, 11, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 170, 193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 285)
[M::mem_pestat] mean and std.dev: (164.50, 45.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 348)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (289.65, 147.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 56, 111)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307)
[M::mem_pestat] mean and std.dev: (55.40, 44.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 405)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 193, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (191.29, 62.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 724266 sequences (100000021 bp) in 2.302 CPU sec, 2.623 real sec...
[M::process] read 723936 sequences (100000105 bp) in 1.932 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305044, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 192, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (239.18, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.45, 147.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 171, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 303)
[M::mem_pestat] mean and std.dev: (143.33, 37.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 363)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724488 reads in 197.915 CPU sec, 6.534 real sec
sorting block 7, #reads = 1102081 mem = 536879296 peak = 994357248
[M::mem_process_seqs] Processed 724364 reads in 113.066 CPU sec, 3.658 real sec
sorting block 8, #reads = 1102134 mem = 536879548 peak = 1013121024
[M::process] read 723450 sequences (100000033 bp) in 2.120 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305211, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 198, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (223.60, 92.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (292.15, 149.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 188, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (195.70, 80.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724892 sequences (100000174 bp) in 1.975 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304210, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 213, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (209.21, 55.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 272, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (295.09, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 178, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402)
[M::mem_pestat] mean and std.dev: (170.93, 36.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305218, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 145, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306)
[M::mem_pestat] mean and std.dev: (148.14, 45.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 370)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (293.38, 149.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 277, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (203.89, 67.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 614)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724266 reads in 302.783 CPU sec, 10.428 real sec
[M::mem_process_seqs] Processed 723450 reads in 211.631 CPU sec, 7.364 real sec
[M::mem_process_seqs] Processed 723936 reads in 221.331 CPU sec, 7.708 real sec
sorting block 9, #reads = 1101322 mem = 536876900 peak = 1013121024
[M::process] read 728190 sequences (100000197 bp) in 2.090 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305103, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 154, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (173.41, 74.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.15, 148.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 191, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (203.69, 81.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 10, #reads = 1100665 mem = 536879575 peak = 1013121024
[M::process] read 727252 sequences (100000213 bp) in 1.944 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307023, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 187, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (104, 299)
[M::mem_pestat] mean and std.dev: (181.62, 29.16)
[M::mem_pestat] low and high boundaries for proper pairs: (65, 338)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.82, 141.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (111, 246)
[M::mem_pestat] mean and std.dev: (179.75, 26.53)
[M::mem_pestat] low and high boundaries for proper pairs: (74, 286)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726988 sequences (100000253 bp) in 1.925 CPU sec, 1.931 real sec...
[M::mem_process_seqs] Processed 724892 reads in 193.134 CPU sec, 6.450 real sec
[M::mem_process_seqs] Processed 728190 reads in 110.463 CPU sec, 3.632 real sec
sorting block 11, #reads = 1101908 mem = 536877708 peak = 1013121024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306909, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 198, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (212.94, 106.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.55, 142.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 178, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (203.77, 101.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726800 sequences (100000276 bp) in 2.526 CPU sec, 2.909 real sec...
[M::process] read 726184 sequences (100000256 bp) in 1.784 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306818, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 204, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (194.80, 70.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.34, 143.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 187, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (193.06, 65.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 635)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727252 reads in 190.086 CPU sec, 6.293 real sec
[M::mem_process_seqs] Processed 726988 reads in 109.498 CPU sec, 3.567 real sec
sorting block 12, #reads = 1104678 mem = 536879035 peak = 1013121024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306621, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 209, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (229.08, 87.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.62, 143.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 168, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (174.00, 56.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725874 sequences (100000068 bp) in 2.195 CPU sec, 2.875 real sec...
[M::process] read 725834 sequences (100000049 bp) in 1.888 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306349, 9, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 177, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 322)
[M::mem_pestat] mean and std.dev: (184.00, 60.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.56, 143.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 193, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (195.96, 62.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726800 reads in 189.024 CPU sec, 6.189 real sec
sorting block 13, #reads = 1104114 mem = 536878698 peak = 1013121024
[M::mem_process_seqs] Processed 726184 reads in 109.653 CPU sec, 3.544 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306032, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 163, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (200.81, 125.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.53, 144.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 168, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 320)
[M::mem_pestat] mean and std.dev: (162.07, 46.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 380)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 14, #reads = 1103683 mem = 536880057 peak = 1071521792
[M::process] read 727218 sequences (100000157 bp) in 2.276 CPU sec, 2.494 real sec...
[M::process] read 726688 sequences (100000029 bp) in 1.686 CPU sec, 1.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306045, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 185, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (188.21, 52.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.25, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 179, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (183.08, 66.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725874 reads in 196.916 CPU sec, 6.550 real sec
[M::mem_process_seqs] Processed 725834 reads in 114.249 CPU sec, 3.781 real sec
sorting block 15, #reads = 1102441 mem = 536876781 peak = 1071521792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306316, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 153, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438)
[M::mem_pestat] mean and std.dev: (180.33, 63.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (278.06, 142.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 157, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404)
[M::mem_pestat] mean and std.dev: (169.00, 61.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 497)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725802 sequences (100000151 bp) in 2.235 CPU sec, 2.662 real sec...
[M::process] read 724876 sequences (100000026 bp) in 1.850 CPU sec, 1.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306415, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 216, 2159)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6193)
[M::mem_pestat] mean and std.dev: (826.23, 1301.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8210)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.08, 143.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 226, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (201.29, 76.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727218 reads in 181.002 CPU sec, 5.951 real sec
[M::mem_process_seqs] Processed 726688 reads in 104.320 CPU sec, 3.414 real sec
sorting block 16, #reads = 1103492 mem = 536878875 peak = 1071521792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305412, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 188, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (185.00, 79.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.28, 145.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 237, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310)
[M::mem_pestat] mean and std.dev: (339.48, 210.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1690)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724554 sequences (100000042 bp) in 2.284 CPU sec, 2.553 real sec...
[M::process] read 724228 sequences (100000073 bp) in 1.736 CPU sec, 1.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305757, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 137, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (188.89, 97.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (288.01, 147.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 163, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (194.77, 80.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725802 reads in 178.166 CPU sec, 5.811 real sec
[M::mem_process_seqs] Processed 724876 reads in 102.593 CPU sec, 3.326 real sec
sorting block 17, #reads = 1103598 mem = 536879629 peak = 1109393408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305118, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 155, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (176.10, 64.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (288.39, 145.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 214, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (234.12, 94.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1102606 mem = 536878433 peak = 1117143040
[M::process] read 724144 sequences (100000142 bp) in 2.065 CPU sec, 2.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305193, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (214.67, 77.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.94, 147.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 181, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (190.88, 69.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723604 sequences (100000016 bp) in 1.794 CPU sec, 2.018 real sec...
[M::mem_process_seqs] Processed 724554 reads in 178.124 CPU sec, 5.880 real sec
[M::mem_process_seqs] Processed 724228 reads in 104.084 CPU sec, 3.419 real sec
sorting block 19, #reads = 1101273 mem = 536879217 peak = 1117143040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305445, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 185, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (194.36, 82.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (290.37, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 195, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (199.16, 71.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723720 sequences (100000113 bp) in 2.198 CPU sec, 2.609 real sec...
[M::process] read 723296 sequences (100000014 bp) in 1.785 CPU sec, 1.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305182, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 166, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (171.45, 59.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (293.06, 148.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 175, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471)
[M::mem_pestat] mean and std.dev: (171.87, 63.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724144 reads in 179.290 CPU sec, 5.856 real sec
[M::mem_process_seqs] Processed 723604 reads in 104.785 CPU sec, 3.415 real sec
sorting block 20, #reads = 1101368 mem = 536878898 peak = 1117143040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304738, 1, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 257, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (229.94, 86.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (293.08, 148.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 166, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (175.19, 71.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 623)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726554 sequences (100000023 bp) in 2.058 CPU sec, 2.390 real sec...
[M::process] read 727570 sequences (100000261 bp) in 1.760 CPU sec, 1.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304552, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 177, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (187.13, 81.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 273, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (295.59, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 233, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (257.74, 116.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723720 reads in 180.354 CPU sec, 5.923 real sec
[M::mem_process_seqs] Processed 723296 reads in 104.436 CPU sec, 3.422 real sec
sorting block 21, #reads = 1101127 mem = 536876693 peak = 1117143040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306617, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 205, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (241.85, 82.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (283.58, 145.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 152, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423)
[M::mem_pestat] mean and std.dev: (181.00, 57.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1100608 mem = 536881830 peak = 1117143040
[M::process] read 727184 sequences (100000238 bp) in 2.266 CPU sec, 2.640 real sec...
[M::process] read 726498 sequences (100000206 bp) in 1.760 CPU sec, 1.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306695, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 224, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (230.22, 87.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.49, 142.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 177, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358)
[M::mem_pestat] mean and std.dev: (181.62, 43.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 432)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726554 reads in 177.887 CPU sec, 5.846 real sec
[M::mem_process_seqs] Processed 727570 reads in 104.751 CPU sec, 3.432 real sec
sorting block 23, #reads = 1103163 mem = 536877541 peak = 1117143040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306910, 9, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 148, 193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 309)
[M::mem_pestat] mean and std.dev: (162.71, 44.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.01, 143.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 208, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 406)
[M::mem_pestat] mean and std.dev: (201.71, 49.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726152 sequences (100000031 bp) in 2.017 CPU sec, 2.229 real sec...
[M::process] read 726224 sequences (100000149 bp) in 1.750 CPU sec, 1.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306324, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 199, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (189.20, 71.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.64, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 221, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 347)
[M::mem_pestat] mean and std.dev: (216.86, 35.46)
[M::mem_pestat] low and high boundaries for proper pairs: (59, 395)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727184 reads in 175.400 CPU sec, 5.678 real sec
[M::mem_process_seqs] Processed 726498 reads in 101.608 CPU sec, 3.340 real sec
sorting block 24, #reads = 1103603 mem = 536881768 peak = 1117143040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306093, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.41, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (247.86, 104.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RR
[M::process] read 725710 sequences (100000246 bp) in 2.446 CPU sec, 2.602 real sec...
[M::process] read 726980 sequences (100000187 bp) in 1.774 CPU sec, 1.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306390, 4, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (285.03, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 276, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (223.64, 66.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726152 reads in 178.130 CPU sec, 5.812 real sec
[M::mem_process_seqs] Processed 726224 reads in 104.194 CPU sec, 3.386 real sec
sorting block 25, #reads = 1103145 mem = 536879230 peak = 1125023744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306073, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 175, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 310)
[M::mem_pestat] mean and std.dev: (165.12, 30.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (284.64, 144.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 195, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (192.33, 62.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 517)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 26, #reads = 1103319 mem = 536879537 peak = 1129537536
[M::process] read 727652 sequences (100000250 bp) in 2.051 CPU sec, 2.262 real sec...
[M::process] read 727150 sequences (100000248 bp) in 1.766 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306409, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 201, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (204.20, 88.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.08, 144.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 173, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (181.67, 70.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725710 reads in 178.692 CPU sec, 5.840 real sec
[M::mem_process_seqs] Processed 726980 reads in 104.335 CPU sec, 3.423 real sec
sorting block 27, #reads = 1102710 mem = 536878684 peak = 1129537536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307092, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 184, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (183.39, 65.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 487)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (277.48, 142.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 194, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359)
[M::mem_pestat] mean and std.dev: (178.18, 45.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 431)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725888 sequences (100000162 bp) in 2.413 CPU sec, 2.641 real sec...
[M::process] read 725620 sequences (100000019 bp) in 2.014 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306553, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 155, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407)
[M::mem_pestat] mean and std.dev: (156.59, 50.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 502)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.28, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 176, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (210.08, 77.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727652 reads in 178.764 CPU sec, 5.802 real sec
[M::mem_process_seqs] Processed 727150 reads in 103.510 CPU sec, 3.373 real sec
sorting block 28, #reads = 1104435 mem = 536880497 peak = 1129537536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306471, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 227, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (202.64, 77.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (284.06, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 724662 sequences (100000201 bp) in 2.085 CPU sec, 2.275 real sec...
[M::process] read 724268 sequences (100000152 bp) in 1.772 CPU sec, 1.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305531, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 189, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444)
[M::mem_pestat] mean and std.dev: (196.25, 76.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (284.91, 144.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 725888 reads in 178.826 CPU sec, 5.792 real sec
[M::mem_process_seqs] Processed 725620 reads in 104.000 CPU sec, 3.337 real sec
sorting block 29, #reads = 1103955 mem = 536877279 peak = 1143074816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305321, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 212, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (210.83, 64.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (289.57, 147.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 204, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (198.11, 58.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 30, #reads = 1102904 mem = 536878897 peak = 1152069632
[M::process] read 723480 sequences (100000007 bp) in 2.549 CPU sec, 2.762 real sec...
[M::process] read 722982 sequences (100000105 bp) in 1.774 CPU sec, 1.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305190, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 189, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (194.33, 72.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (291.40, 147.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 227, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (226.15, 69.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724662 reads in 180.284 CPU sec, 5.891 real sec
[M::mem_process_seqs] Processed 724268 reads in 104.882 CPU sec, 3.430 real sec
sorting block 31, #reads = 1102055 mem = 536879400 peak = 1152069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304938, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 226, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (244.67, 97.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (293.50, 147.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 245, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (235.62, 96.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723026 sequences (100000140 bp) in 1.987 CPU sec, 2.413 real sec...
[M::process] read 722602 sequences (100000088 bp) in 1.803 CPU sec, 1.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304191, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 242, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (367.65, 336.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (294.90, 147.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 182, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (178.06, 58.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 650)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723480 reads in 179.970 CPU sec, 5.858 real sec
sorting block 32, #reads = 1101506 mem = 536881065 peak = 1152069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304860, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 220, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (203.76, 103.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 628)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (295.77, 148.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 269, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (293.56, 88.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723026 reads in 96.658 CPU sec, 3.094 real sec
[M::process] read 728098 sequences (100000287 bp) in 2.208 CPU sec, 2.230 real sec...
[M::mem_process_seqs] Processed 722982 reads in 239.294 CPU sec, 7.700 real sec
sorting block 33, #reads = 1100641 mem = 536877864 peak = 1152069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 304268, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 192, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (201.82, 102.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (297.60, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 224, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 453)
[M::mem_pestat] mean and std.dev: (223.85, 62.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727100 sequences (100000081 bp) in 1.991 CPU sec, 2.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307105, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 167, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (209.18, 96.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (278.27, 142.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 222, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (197.75, 69.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726454 sequences (100000133 bp) in 1.829 CPU sec, 1.841 real sec...
[M::mem_process_seqs] Processed 722602 reads in 190.446 CPU sec, 6.114 real sec
[M::mem_process_seqs] Processed 728098 reads in 106.307 CPU sec, 3.397 real sec
sorting block 34, #reads = 1099607 mem = 536880090 peak = 1152069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306311, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (280.76, 143.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 726384 sequences (100000077 bp) in 2.260 CPU sec, 2.686 real sec...
[M::process] read 726124 sequences (100000124 bp) in 1.826 CPU sec, 1.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 306077, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 173, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (197.86, 67.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 728)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.82, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 187, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (181.06, 50.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727100 reads in 179.131 CPU sec, 5.799 real sec
[M::mem_process_seqs] Processed 726454 reads in 104.376 CPU sec, 3.353 real sec
sorting block 35, #reads = 1103930 mem = 536878568 peak = 1153695744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305885, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 230, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (230.73, 105.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.62, 144.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 168, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (171.60, 57.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725784 sequences (100000032 bp) in 1.985 CPU sec, 2.236 real sec...
sorting block 36, #reads = 1103630 mem = 536879190 peak = 1169199104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305825, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 254, 1339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3673)
[M::mem_pestat] mean and std.dev: (577.73, 647.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.90, 144.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 223, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (248.55, 106.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726384 reads in 177.132 CPU sec, 5.802 real sec
[M::process] read 726976 sequences (100000199 bp) in 1.828 CPU sec, 1.968 real sec...
[M::mem_process_seqs] Processed 726124 reads in 104.859 CPU sec, 3.438 real sec
sorting block 37, #reads = 1103388 mem = 536878393 peak = 1169199104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 305951, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 182, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (217.44, 110.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (283.99, 144.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 254, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (228.65, 66.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728204 sequences (100000172 bp) in 2.257 CPU sec, 2.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306209, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 184, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (203.08, 82.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.90, 143.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 205, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (189.33, 78.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728098 sequences (100000173 bp) in 2.114 CPU sec, 2.148 real sec...
[M::mem_process_seqs] Processed 725784 reads in 178.983 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 726976 reads in 104.740 CPU sec, 3.331 real sec
sorting block 38, #reads = 1102709 mem = 536879956 peak = 1169199104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307451, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 248, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (190.59, 57.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (276.33, 142.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 209, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (231.00, 80.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727816 sequences (100000039 bp) in 2.078 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307342, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 176, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (192.86, 73.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.71, 142.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 204, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (203.69, 71.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727754 sequences (100000231 bp) in 1.862 CPU sec, 1.993 real sec...
[M::mem_process_seqs] Processed 728204 reads in 177.707 CPU sec, 5.769 real sec
sorting block 39, #reads = 1104063 mem = 536879844 peak = 1176928256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306811, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 262, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (240.10, 114.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (277.46, 142.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 191, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (215.00, 73.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727816 reads in 96.164 CPU sec, 3.097 real sec
[M::process] read 727086 sequences (100000128 bp) in 2.283 CPU sec, 2.318 real sec...
[M::mem_process_seqs] Processed 728098 reads in 246.778 CPU sec, 7.978 real sec
sorting block 40, #reads = 1104814 mem = 536879365 peak = 1176928256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307602, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 226, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (202.50, 72.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 658)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.71, 143.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 255, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (222.83, 85.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725468 sequences (100000024 bp) in 2.223 CPU sec, 2.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306989, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 177, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (204.09, 79.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (280.60, 144.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 195, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426)
[M::mem_pestat] mean and std.dev: (186.80, 64.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 521)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727754 reads in 186.764 CPU sec, 6.060 real sec
[M::mem_process_seqs] Processed 727086 reads in 104.233 CPU sec, 3.346 real sec
[M::process] read 724036 sequences (100000195 bp) in 2.148 CPU sec, 2.321 real sec...
sorting block 41, #reads = 1104867 mem = 536877029 peak = 1187381248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306136, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 271, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (217.71, 82.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (285.28, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 174, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (175.86, 63.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 527)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723376 sequences (100000027 bp) in 2.116 CPU sec, 2.595 real sec...
sorting block 42, #reads = 1104399 mem = 536881744 peak = 1187381248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305316, 0, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 234, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (225.78, 79.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (289.06, 145.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 181, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (187.45, 48.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 722776 sequences (100000030 bp) in 1.718 CPU sec, 1.744 real sec...
[M::mem_process_seqs] Processed 725468 reads in 179.489 CPU sec, 5.903 real sec
[M::mem_process_seqs] Processed 724036 reads in 106.422 CPU sec, 3.502 real sec
sorting block 43, #reads = 1102486 mem = 536878718 peak = 1187381248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304894, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 190, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495)
[M::mem_pestat] mean and std.dev: (192.82, 65.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (292.95, 147.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 217, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 392)
[M::mem_pestat] mean and std.dev: (190.31, 59.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 722506 sequences (100000270 bp) in 2.184 CPU sec, 2.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 304296, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 183, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (237.04, 120.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (294.46, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 178, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (190.88, 65.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 696)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723376 reads in 177.341 CPU sec, 5.814 real sec
[M::process] read 727678 sequences (100000059 bp) in 1.910 CPU sec, 1.923 real sec...
[M::mem_process_seqs] Processed 722776 reads in 104.482 CPU sec, 3.374 real sec
sorting block 44, #reads = 1101234 mem = 536881389 peak = 1187381248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304270, 10, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 126, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308)
[M::mem_pestat] mean and std.dev: (138.88, 41.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (297.25, 149.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 165, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239)
[M::mem_pestat] mean and std.dev: (216.20, 206.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1637)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 262, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 426)
[M::mem_pestat] mean and std.dev: (246.91, 57.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 503)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 728530 sequences (100000165 bp) in 1.911 CPU sec, 2.234 real sec...
[M::process] read 727682 sequences (100000181 bp) in 1.882 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306638, 7, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 197, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (208.35, 73.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (280.44, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 215, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (207.14, 111.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722506 reads in 180.967 CPU sec, 5.890 real sec
[M::mem_process_seqs] Processed 727678 reads in 105.892 CPU sec, 3.402 real sec
sorting block 45, #reads = 1099616 mem = 536877791 peak = 1187381248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307399, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 179, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366)
[M::mem_pestat] mean and std.dev: (171.62, 61.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 442)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (278.86, 145.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 273, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (244.50, 110.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727366 sequences (100000158 bp) in 2.186 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 305937, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 207, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (236.09, 91.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (280.34, 145.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 216, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 405)
[M::mem_pestat] mean and std.dev: (206.07, 51.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727234 sequences (100000074 bp) in 1.927 CPU sec, 1.933 real sec...
[M::mem_process_seqs] Processed 728530 reads in 183.770 CPU sec, 5.979 real sec
sorting block 46, #reads = 1102893 mem = 536878884 peak = 1187381248
[M::mem_process_seqs] Processed 727682 reads in 107.541 CPU sec, 3.434 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306392, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (209.95, 80.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.48, 145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 197, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (221.13, 105.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1104664 mem = 536880011 peak = 1187381248
[M::process] read 726372 sequences (100000063 bp) in 2.258 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306311, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 270, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (239.50, 108.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (283.07, 146.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 184, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (214.56, 117.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726314 sequences (100000113 bp) in 1.848 CPU sec, 1.857 real sec...
[M::mem_process_seqs] Processed 727366 reads in 182.419 CPU sec, 5.945 real sec
[M::mem_process_seqs] Processed 727234 reads in 106.507 CPU sec, 3.403 real sec
sorting block 48, #reads = 1103616 mem = 536878768 peak = 1187381248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306153, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 214, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (221.90, 72.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.62, 146.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 192, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (201.20, 78.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726170 sequences (100000167 bp) in 2.400 CPU sec, 2.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305788, 9, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 139, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417)
[M::mem_pestat] mean and std.dev: (161.33, 71.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (284.73, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 221, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (241.17, 95.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726372 reads in 181.429 CPU sec, 5.914 real sec
[M::process] read 726558 sequences (100000040 bp) in 1.912 CPU sec, 1.917 real sec...
[M::mem_process_seqs] Processed 726314 reads in 106.199 CPU sec, 3.396 real sec
sorting block 49, #reads = 1103841 mem = 536880729 peak = 1187381248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305502, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 168, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 337)
[M::mem_pestat] mean and std.dev: (162.28, 48.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (284.10, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 215, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (182.22, 61.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725588 sequences (100000121 bp) in 2.242 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306045, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 231, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (234.16, 105.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.75, 145.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 177, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (198.14, 53.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725598 sequences (100000173 bp) in 1.915 CPU sec, 1.921 real sec...
[M::mem_process_seqs] Processed 726170 reads in 182.331 CPU sec, 5.953 real sec
sorting block 50, #reads = 1103220 mem = 536880239 peak = 1197817856
[M::mem_process_seqs] Processed 726558 reads in 106.290 CPU sec, 3.425 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306331, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 211, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (195.00, 58.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (284.92, 145.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 268, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (205.92, 82.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 51, #reads = 1102393 mem = 536877737 peak = 1199226880
[M::process] read 725178 sequences (100000206 bp) in 2.229 CPU sec, 2.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305508, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 158, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 301)
[M::mem_pestat] mean and std.dev: (158.33, 30.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 356)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.44, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 239, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (237.89, 74.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725268 sequences (100000088 bp) in 1.933 CPU sec, 1.936 real sec...
[M::mem_process_seqs] Processed 725588 reads in 182.719 CPU sec, 5.997 real sec
[M::mem_process_seqs] Processed 725598 reads in 107.210 CPU sec, 3.509 real sec
sorting block 52, #reads = 1103277 mem = 536880711 peak = 1199226880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 305536, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 196, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465)
[M::mem_pestat] mean and std.dev: (199.84, 67.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (289.71, 149.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 176, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (178.12, 44.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725274 sequences (100000108 bp) in 2.278 CPU sec, 2.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305910, 7, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 215, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (208.72, 87.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 647)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::process] read 725124 sequences (100000056 bp) in 1.851 CPU sec, 1.871 real sec...
[M::mem_pestat] mean and std.dev: (289.22, 148.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 188, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (191.83, 61.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725178 reads in 180.603 CPU sec, 5.914 real sec
[M::mem_process_seqs] Processed 725268 reads in 104.514 CPU sec, 3.383 real sec
sorting block 53, #reads = 1102753 mem = 536879098 peak = 1199226880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305776, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 209, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (214.56, 56.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (289.71, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 207, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 312)
[M::mem_pestat] mean and std.dev: (190.40, 32.43)
[M::mem_pestat] low and high boundaries for proper pairs: (61, 353)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725408 sequences (100000075 bp) in 2.241 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 305714, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (291.39, 151.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 202, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 402)
[M::mem_pestat] mean and std.dev: (188.25, 52.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 482)
[M::mem_pestat] skip orientation RR
[M::process] read 725396 sequences (100000202 bp) in 1.899 CPU sec, 2.097 real sec...
[M::mem_process_seqs] Processed 725274 reads in 182.219 CPU sec, 5.878 real sec
sorting block 54, #reads = 1102248 mem = 536876850 peak = 1202266112
[M::mem_process_seqs] Processed 725124 reads in 104.161 CPU sec, 3.331 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305586, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 198, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (180.35, 85.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (292.36, 152.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 204, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (195.15, 72.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 55, #reads = 1102595 mem = 536880334 peak = 1202266112
[M::process] read 728636 sequences (100000196 bp) in 2.161 CPU sec, 2.481 real sec...
[M::process] read 728520 sequences (100000200 bp) in 1.869 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305229, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 197, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (188.92, 66.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (292.48, 153.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 162, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (167.11, 48.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725408 reads in 183.680 CPU sec, 5.964 real sec
[M::mem_process_seqs] Processed 725396 reads in 106.415 CPU sec, 3.447 real sec
sorting block 56, #reads = 1102880 mem = 536882265 peak = 1202266112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307051, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 149, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (167.20, 81.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 553)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 252, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (280.54, 148.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 167, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411)
[M::mem_pestat] mean and std.dev: (165.62, 48.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 505)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727738 sequences (100000174 bp) in 2.226 CPU sec, 2.583 real sec...
[M::process] read 726822 sequences (100000112 bp) in 1.920 CPU sec, 1.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307642, 1, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 227, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (199.91, 53.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (278.80, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 150, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (188.06, 82.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728636 reads in 181.625 CPU sec, 5.881 real sec
[M::mem_process_seqs] Processed 728520 reads in 105.435 CPU sec, 3.368 real sec
sorting block 57, #reads = 1103050 mem = 536878710 peak = 1202266112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307082, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 173, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 319)
[M::mem_pestat] mean and std.dev: (170.38, 56.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (280.02, 144.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 267, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (266.30, 109.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726992 sequences (100000208 bp) in 2.103 CPU sec, 2.411 real sec...
[M::process] read 726482 sequences (100000089 bp) in 1.874 CPU sec, 1.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306138, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 144, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (157.88, 55.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (281.62, 144.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 217, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 435)
[M::mem_pestat] mean and std.dev: (226.54, 75.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 529)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727738 reads in 180.189 CPU sec, 5.889 real sec
sorting block 58, #reads = 1105160 mem = 536881762 peak = 1205731328
[M::mem_process_seqs] Processed 726822 reads in 105.162 CPU sec, 3.428 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306693, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.52, 145.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 257, 1767)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4851)
[M::mem_pestat] mean and std.dev: (640.67, 706.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6393)
[M::mem_pestat] skip orientation RR
sorting block 59, #reads = 1103740 mem = 536879481 peak = 1209503744
[M::process] read 726260 sequences (100000144 bp) in 2.488 CPU sec, 2.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305928, 0, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 257, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (222.83, 86.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.67, 144.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 167, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (159.11, 44.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726718 sequences (100000184 bp) in 1.897 CPU sec, 1.922 real sec...
[M::mem_process_seqs] Processed 726992 reads in 182.437 CPU sec, 5.989 real sec
[M::mem_process_seqs] Processed 726482 reads in 106.732 CPU sec, 3.523 real sec
sorting block 60, #reads = 1103820 mem = 536880201 peak = 1209503744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305560, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 206, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 425)
[M::mem_pestat] mean and std.dev: (199.00, 53.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 506)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (284.50, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 216, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (211.58, 90.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727726 sequences (100000119 bp) in 2.169 CPU sec, 2.347 real sec...
[M::process] read 726608 sequences (100000173 bp) in 1.862 CPU sec, 1.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306353, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 209, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (222.00, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.49, 144.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 174, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (192.64, 78.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726260 reads in 182.152 CPU sec, 5.933 real sec
[M::mem_process_seqs] Processed 726718 reads in 105.787 CPU sec, 3.465 real sec
sorting block 61, #reads = 1103183 mem = 536878675 peak = 1209503744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306890, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 196, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (201.06, 68.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.40, 143.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 244, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (260.00, 94.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725746 sequences (100000096 bp) in 2.267 CPU sec, 2.467 real sec...
[M::process] read 725484 sequences (100000198 bp) in 1.923 CPU sec, 1.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305931, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 186, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (169.12, 48.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.95, 145.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 192, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (192.63, 59.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727726 reads in 184.076 CPU sec, 5.958 real sec
sorting block 62, #reads = 1103499 mem = 536880650 peak = 1214746624
[M::mem_process_seqs] Processed 726608 reads in 106.905 CPU sec, 3.441 real sec
sorting block 63, #reads = 1104130 mem = 536879423 peak = 1215782912
[M::process] read 725576 sequences (100000127 bp) in 2.097 CPU sec, 2.341 real sec...
[M::process] read 725288 sequences (100000226 bp) in 1.770 CPU sec, 1.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305645, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 213, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (184.75, 72.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (288.85, 148.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 261, 4030)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11786)
[M::mem_pestat] mean and std.dev: (1357.22, 1800.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15664)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725484 reads in 99.439 CPU sec, 3.264 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305895, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 220, 1083)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2887)
[M::mem_pestat] mean and std.dev: (349.58, 334.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3789)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.13, 146.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305576, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 162, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (173.00, 73.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (289.44, 149.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 196, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 392)
[M::mem_pestat] mean and std.dev: (197.20, 54.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725746 reads in 278.051 CPU sec, 9.076 real sec
[M::process] read 725132 sequences (100000075 bp) in 2.066 CPU sec, 2.080 real sec...
[M::mem_process_seqs] Processed 725576 reads in 115.713 CPU sec, 3.756 real sec
sorting block 64, #reads = 1101648 mem = 536877712 peak = 1215782912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305305, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 173, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (173.57, 51.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (289.49, 149.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 175, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (208.61, 80.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 65, #reads = 1102793 mem = 536878939 peak = 1215782912
[M::process] read 724978 sequences (100000248 bp) in 2.259 CPU sec, 2.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305132, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 227, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 504)
[M::mem_pestat] mean and std.dev: (229.80, 81.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (292.00, 151.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 243, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (220.43, 56.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 567)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725288 reads in 184.715 CPU sec, 6.058 real sec
[M::process] read 725004 sequences (100000033 bp) in 1.804 CPU sec, 1.827 real sec...
[M::mem_process_seqs] Processed 725132 reads in 107.181 CPU sec, 3.483 real sec
sorting block 66, #reads = 1101475 mem = 536878253 peak = 1215782912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305300, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 163, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111)
[M::mem_pestat] mean and std.dev: (218.40, 139.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1438)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (292.41, 151.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 237, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (219.17, 76.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727886 sequences (100000232 bp) in 2.269 CPU sec, 2.860 real sec...
[M::process] read 728164 sequences (100000255 bp) in 1.797 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305763, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 192, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (220.68, 136.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (293.22, 153.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 214, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390)
[M::mem_pestat] mean and std.dev: (190.85, 45.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724978 reads in 178.756 CPU sec, 5.834 real sec
[M::mem_process_seqs] Processed 725004 reads in 103.430 CPU sec, 3.342 real sec
sorting block 67, #reads = 1102101 mem = 536879498 peak = 1215782912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307338, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 235, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (215.73, 106.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (283.08, 149.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 174, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (185.00, 61.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727444 sequences (100000142 bp) in 2.184 CPU sec, 2.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306795, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 236, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (247.68, 89.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (279.82, 145.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 204, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (183.21, 66.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727028 sequences (100000232 bp) in 1.918 CPU sec, 1.924 real sec...
[M::mem_process_seqs] Processed 727886 reads in 182.521 CPU sec, 5.956 real sec
[M::mem_process_seqs] Processed 728164 reads in 108.212 CPU sec, 3.473 real sec
sorting block 68, #reads = 1101628 mem = 536880094 peak = 1215782912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306533, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 178, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (176.91, 60.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.25, 145.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726718 sequences (100000089 bp) in 2.428 CPU sec, 2.588 real sec...
sorting block 69, #reads = 1104770 mem = 536877583 peak = 1218203648
[M::process] read 726486 sequences (100000053 bp) in 1.859 CPU sec, 1.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306116, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 232, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (157, 307)
[M::mem_pestat] mean and std.dev: (242.88, 18.94)
[M::mem_pestat] low and high boundaries for proper pairs: (127, 337)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (282.94, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 148, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (210.46, 129.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727444 reads in 183.398 CPU sec, 5.963 real sec
[M::mem_process_seqs] Processed 727028 reads in 106.679 CPU sec, 3.454 real sec
sorting block 70, #reads = 1103877 mem = 536880193 peak = 1218203648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305847, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (110, 143, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (163.54, 69.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.08, 145.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 199, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (221.09, 81.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726576 sequences (100000156 bp) in 2.290 CPU sec, 2.648 real sec...
[M::process] read 727766 sequences (100000023 bp) in 1.874 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305497, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 174, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (207.43, 100.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (283.23, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 169, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (174.55, 78.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 515)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726718 reads in 183.294 CPU sec, 6.025 real sec
[M::mem_process_seqs] Processed 726486 reads in 106.411 CPU sec, 3.508 real sec
sorting block 71, #reads = 1103480 mem = 536881090 peak = 1218203648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306218, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 264, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442)
[M::mem_pestat] mean and std.dev: (334.91, 251.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1883)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (284.47, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 198, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (175.38, 54.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728648 sequences (100000140 bp) in 2.177 CPU sec, 2.316 real sec...
[M::process] read 727714 sequences (100000170 bp) in 1.926 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306574, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 156, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (173.20, 65.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (279.08, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 226, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 395)
[M::mem_pestat] mean and std.dev: (203.08, 52.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726576 reads in 184.107 CPU sec, 5.948 real sec
[M::mem_process_seqs] Processed 727766 reads in 107.511 CPU sec, 3.437 real sec
sorting block 72, #reads = 1103268 mem = 536879449 peak = 1228390400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307356, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 235, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 478)
[M::mem_pestat] mean and std.dev: (224.91, 71.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (275.63, 142.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 148, 159)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 227)
[M::mem_pestat] mean and std.dev: (144.80, 31.02)
[M::mem_pestat] low and high boundaries for proper pairs: (21, 269)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 73, #reads = 1103805 mem = 536879075 peak = 1235660800
[M::process] read 727056 sequences (100000232 bp) in 2.235 CPU sec, 2.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306606, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 179, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (231.00, 116.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.67, 144.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 237, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (218.50, 74.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726380 sequences (100000153 bp) in 1.810 CPU sec, 1.987 real sec...
[M::mem_process_seqs] Processed 728648 reads in 184.993 CPU sec, 6.028 real sec
[M::mem_process_seqs] Processed 727714 reads in 109.193 CPU sec, 3.536 real sec
sorting block 74, #reads = 1105443 mem = 536877605 peak = 1235660800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306414, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 182, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (193.45, 98.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.49, 145.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 195, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.69, 98.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725364 sequences (100000251 bp) in 2.219 CPU sec, 2.591 real sec...
[M::process] read 724890 sequences (100000106 bp) in 1.889 CPU sec, 1.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306169, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 186, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (202.33, 73.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 772)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (285.83, 148.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 339, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1493)
[M::mem_pestat] mean and std.dev: (360.92, 175.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1933)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727056 reads in 183.160 CPU sec, 5.948 real sec
[M::mem_process_seqs] Processed 726380 reads in 105.081 CPU sec, 3.365 real sec
sorting block 75, #reads = 1102367 mem = 536879475 peak = 1235660800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305338, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 158, 184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 280)
[M::mem_pestat] mean and std.dev: (161.18, 36.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 328)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (288.66, 148.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 177, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365)
[M::mem_pestat] mean and std.dev: (178.44, 46.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 442)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724320 sequences (100000112 bp) in 2.160 CPU sec, 2.413 real sec...
[M::process] read 724742 sequences (100000267 bp) in 1.907 CPU sec, 1.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305605, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 161, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (188.55, 94.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (289.93, 149.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 188, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (192.79, 83.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725364 reads in 182.266 CPU sec, 5.909 real sec
[M::mem_process_seqs] Processed 724890 reads in 107.157 CPU sec, 3.422 real sec
sorting block 76, #reads = 1103307 mem = 536879909 peak = 1239420928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305433, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 167, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (171.33, 72.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (292.77, 150.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (252.36, 96.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 77, #reads = 1102036 mem = 536880465 peak = 1249181696
[M::process] read 724982 sequences (100000145 bp) in 2.256 CPU sec, 2.504 real sec...
[M::process] read 727674 sequences (100000102 bp) in 1.914 CPU sec, 1.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305115, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 170, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (188.96, 67.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 638)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (292.89, 151.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 202, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (188.11, 50.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 457)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724320 reads in 179.910 CPU sec, 5.872 real sec
[M::mem_process_seqs] Processed 724742 reads in 104.357 CPU sec, 3.392 real sec
sorting block 78, #reads = 1101430 mem = 536879945 peak = 1249181696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305929, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 223, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (223.14, 95.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (293.63, 153.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 226, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (106, 351)
[M::mem_pestat] mean and std.dev: (230.80, 33.65)
[M::mem_pestat] low and high boundaries for proper pairs: (57, 400)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727800 sequences (100000000 bp) in 2.251 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306513, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 197, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (230.23, 101.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 732)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (283.79, 149.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 191, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 356)
[M::mem_pestat] mean and std.dev: (188.90, 40.31)
[M::mem_pestat] low and high boundaries for proper pairs: (2, 415)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727082 sequences (100000037 bp) in 1.909 CPU sec, 1.929 real sec...
[M::mem_process_seqs] Processed 724982 reads in 178.708 CPU sec, 5.829 real sec
[M::mem_process_seqs] Processed 727674 reads in 105.918 CPU sec, 3.426 real sec
sorting block 79, #reads = 1101651 mem = 536879746 peak = 1249181696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307418, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 182, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (178.13, 58.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.42, 145.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 173, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 347)
[M::mem_pestat] mean and std.dev: (172.33, 44.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726684 sequences (100000146 bp) in 2.538 CPU sec, 2.759 real sec...
[M::process] read 726502 sequences (100000210 bp) in 1.774 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306646, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 204, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (198.72, 83.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 259, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (283.32, 146.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727800 reads in 181.827 CPU sec, 5.847 real sec
[M::mem_process_seqs] Processed 727082 reads in 105.869 CPU sec, 3.368 real sec
sorting block 80, #reads = 1103930 mem = 536878321 peak = 1249181696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305677, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 191, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (194.96, 77.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (283.47, 146.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 240, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (263.85, 110.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 81, #reads = 1104441 mem = 536879731 peak = 1261350912
[M::process] read 726520 sequences (100000112 bp) in 2.263 CPU sec, 2.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305893, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 192, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (222.90, 94.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (283.58, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 166, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 274)
[M::mem_pestat] mean and std.dev: (141.33, 31.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 324)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727132 sequences (100000255 bp) in 1.728 CPU sec, 1.995 real sec...
[M::mem_process_seqs] Processed 726684 reads in 186.141 CPU sec, 6.059 real sec
[M::mem_process_seqs] Processed 726502 reads in 107.228 CPU sec, 3.469 real sec
sorting block 82, #reads = 1102783 mem = 536879100 peak = 1261350912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305765, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 155, 184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (85, 250)
[M::mem_pestat] mean and std.dev: (151.00, 40.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 315)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.21, 145.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 137, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (176.77, 87.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727876 sequences (100000001 bp) in 2.427 CPU sec, 2.711 real sec...
[M::process] read 728536 sequences (100000053 bp) in 1.923 CPU sec, 1.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306330, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 228, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (224.58, 86.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.29, 144.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (106, 153, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (153.90, 46.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726520 reads in 181.020 CPU sec, 5.845 real sec
[M::mem_process_seqs] Processed 727132 reads in 105.805 CPU sec, 3.405 real sec
sorting block 83, #reads = 1103725 mem = 536878265 peak = 1261350912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 307172, 8, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 277, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (239.09, 126.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.74, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 356, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214)
[M::mem_pestat] mean and std.dev: (376.90, 201.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1544)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728498 sequences (100000131 bp) in 2.286 CPU sec, 2.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307292, 8, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (275.04, 141.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 171, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416)
[M::mem_pestat] mean and std.dev: (173.79, 59.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] skip orientation RR
[M::process] read 727854 sequences (100000011 bp) in 1.986 CPU sec, 1.987 real sec...
[M::mem_process_seqs] Processed 727876 reads in 180.085 CPU sec, 5.851 real sec
sorting block 84, #reads = 1103362 mem = 536879547 peak = 1261350912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307639, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.87, 142.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 226, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444)
[M::mem_pestat] mean and std.dev: (202.25, 47.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] skip orientation RR
[M::process] read 727520 sequences (100000243 bp) in 1.999 CPU sec, 2.027 real sec...
[M::mem_process_seqs] Processed 728498 reads in 100.116 CPU sec, 3.358 real sec
sorting block 85, #reads = 1103874 mem = 536877584 peak = 1261350912
[M::mem_process_seqs] Processed 728536 reads in 244.168 CPU sec, 8.028 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306787, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 222, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (234.83, 100.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.12, 142.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 152, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342)
[M::mem_pestat] mean and std.dev: (161.33, 58.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 417)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726502 sequences (100000153 bp) in 1.848 CPU sec, 1.962 real sec...
[M::process] read 725670 sequences (100000039 bp) in 1.389 CPU sec, 1.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306716, 2, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 190, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (238.41, 99.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (279.61, 144.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 158, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (167.15, 68.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727854 reads in 174.924 CPU sec, 5.618 real sec
[M::mem_process_seqs] Processed 727520 reads in 84.455 CPU sec, 2.674 real sec
sorting block 86, #reads = 1104736 mem = 536876776 peak = 1261350912
sorting block 87, #reads = 1103669 mem = 536879237 peak = 1261350912
[M::process] read 724474 sequences (100000210 bp) in 1.875 CPU sec, 1.915 real sec...
[M::process] read 724246 sequences (100000043 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305856, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 223, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (319.23, 226.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 263, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (287.00, 147.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 210, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453)
[M::mem_pestat] mean and std.dev: (191.85, 80.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 551)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725670 reads in 72.912 CPU sec, 2.311 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306300, 6, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 162, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (156.06, 46.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.72, 144.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 210, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 370)
[M::mem_pestat] mean and std.dev: (200.35, 51.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 436)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 304989, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 184, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (203.71, 113.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (292.05, 149.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726502 reads in 218.176 CPU sec, 6.916 real sec
[M::process] read 724308 sequences (100000231 bp) in 1.964 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 724474 reads in 94.242 CPU sec, 3.005 real sec
sorting block 88, #reads = 1103469 mem = 536879442 peak = 1261350912
[M::process] read 259752 sequences (35827624 bp) in 1.097 CPU sec, 1.180 real sec...
sorting block 89, #reads = 1101465 mem = 536880142 peak = 1261350912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304904, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 183, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (161.43, 48.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (294.07, 151.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 183, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (205.65, 70.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 109210, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 207, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 312)
[M::mem_pestat] mean and std.dev: (181.67, 53.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (294.58, 154.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304700, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 220, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (187.77, 62.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (294.30, 152.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 178, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 329)
[M::mem_pestat] mean and std.dev: (149.38, 35.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 392)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724246 reads in 192.549 CPU sec, 6.224 real sec
[M::mem_process_seqs] Processed 259752 reads in 118.559 CPU sec, 3.842 real sec
[M::mem_process_seqs] Processed 724308 reads in 128.992 CPU sec, 4.204 real sec
sorting block 90, #reads = 1101520 mem = 536878414 peak = 1261350912
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000606a6
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef 40417f5e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 100460662
overall: 69875699712 mem 14911.643 user 354.011 sys 516.093 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.SH032_HG514.L002\tPU:L002\tSM:SH032_HG514\tLB:SH032_HG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.SH032_HG514_R1.fastq.gz 0004.SH032_HG514_R2.fastq.gz
[main] Real time: 516.129 sec; CPU: 15265.658 sec
sorting block 91, #reads = 676360 mem = 329826505 peak = 1261350912
uniq header count 1
merging 92 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000606a6
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef 40417f5e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz
threads: 31 max 128
algo: util-sort
license: sentieon:util=1
output file size: 4441369848
output reads: 101045217
bam_mem_sort: 92 calls 89.069 user 1.002 sys 93.200 real
bam_write: 92 calls 59.094 user 3.582 sys 137.789 real
execute: 1 calls 29.805 user 2.181 sys 549.888 real
merge_files: 1 calls 29.785 user 2.161 sys 33.222 real
parse_chunk: 1466 calls 95.797 user 18.686 sys 147.788 real
read_chunk: 3969 calls 44.078 user 21.959 sys 443.413 real
sort_block: 1 calls 31.835 user 21.433 sys 436.124 real
write_chunk: 2501 calls 29.362 user 1.973 sys 32.380 real
overall: 2685595648 mem 1283.368 user 62.518 sys 549.910 real
ls: cannot access '*.cram': No such file or directory