This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o D0001483_22q.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2138.284 CPU sec, 77.007 real sec
[M::process] read 716304 sequences (100000073 bp) in 1.449 CPU sec, 1.453 real sec...
[M::process] read 716126 sequences (100000159 bp) in 2.042 CPU sec, 2.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304957, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 349)
[M::mem_pestat] mean and std.dev: (176.32, 61.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715806 sequences (100000034 bp) in 1.894 CPU sec, 1.947 real sec...
[M::process] read 715844 sequences (100000135 bp) in 2.149 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304127, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (185.08, 71.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716304 reads in 210.542 CPU sec, 6.805 real sec
[M::mem_process_seqs] Processed 716126 reads in 101.941 CPU sec, 3.337 real sec
sorting block 0, #reads = 1093086 mem = 536879886 peak = 610590720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304434, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.52, 70.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715820 sequences (100000006 bp) in 2.541 CPU sec, 2.598 real sec...
[M::process] read 715810 sequences (100000210 bp) in 1.489 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304717, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (186.21, 72.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715806 reads in 165.468 CPU sec, 5.264 real sec
[M::mem_process_seqs] Processed 715844 reads in 94.122 CPU sec, 3.013 real sec
sorting block 1, #reads = 1092678 mem = 536877968 peak = 770236416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304247, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (187.14, 73.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715746 sequences (100000065 bp) in 2.698 CPU sec, 2.764 real sec...
[M::process] read 715920 sequences (100000070 bp) in 2.172 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304566, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (187.71, 74.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715820 reads in 182.797 CPU sec, 5.825 real sec
[M::mem_process_seqs] Processed 715810 reads in 109.203 CPU sec, 3.485 real sec
sorting block 2, #reads = 1092516 mem = 536880501 peak = 917368832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304774, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (185.80, 72.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715870 sequences (100000105 bp) in 2.506 CPU sec, 2.552 real sec...
[M::process] read 715948 sequences (100000049 bp) in 2.011 CPU sec, 2.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304912, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (178.98, 64.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 435)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715746 reads in 189.666 CPU sec, 6.070 real sec
sorting block 3, #reads = 1092287 mem = 536880738 peak = 1001304064
[M::mem_process_seqs] Processed 715920 reads in 109.448 CPU sec, 3.476 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304529, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 4, #reads = 1092747 mem = 536877572 peak = 1015496704
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (178.91, 64.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 435)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 90, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938)
[M::mem_pestat] mean and std.dev: (140.50, 207.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2579)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715876 sequences (100000003 bp) in 2.547 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304861, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 349)
[M::mem_pestat] mean and std.dev: (176.58, 61.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715956 sequences (100000286 bp) in 2.049 CPU sec, 2.058 real sec...
[M::mem_process_seqs] Processed 715870 reads in 191.934 CPU sec, 6.164 real sec
[M::mem_process_seqs] Processed 715948 reads in 111.598 CPU sec, 3.550 real sec
sorting block 5, #reads = 1092689 mem = 536876867 peak = 1015496704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304541, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 361)
[M::mem_pestat] mean and std.dev: (179.86, 65.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 440)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715944 sequences (100000243 bp) in 2.558 CPU sec, 2.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 304750, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373)
[M::mem_pestat] mean and std.dev: (182.61, 68.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 456)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715944 sequences (100000273 bp) in 2.031 CPU sec, 2.049 real sec...
[M::mem_process_seqs] Processed 715876 reads in 192.714 CPU sec, 6.178 real sec
[M::mem_process_seqs] Processed 715956 reads in 111.590 CPU sec, 3.544 real sec
sorting block 6, #reads = 1093260 mem = 536880236 peak = 1015496704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 305152, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 361)
[M::mem_pestat] mean and std.dev: (179.79, 65.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 440)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715952 sequences (100000083 bp) in 2.701 CPU sec, 2.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304466, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 361)
[M::mem_pestat] mean and std.dev: (180.05, 65.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 74, 81)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193)
[M::mem_pestat] mean and std.dev: (61.40, 39.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 249)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715786 sequences (100000036 bp) in 2.090 CPU sec, 2.098 real sec...
[M::mem_process_seqs] Processed 715944 reads in 193.896 CPU sec, 6.211 real sec
[M::mem_process_seqs] Processed 715944 reads in 111.565 CPU sec, 3.542 real sec
sorting block 7, #reads = 1093086 mem = 536878901 peak = 1015496704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304690, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 361)
[M::mem_pestat] mean and std.dev: (179.94, 65.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 8, #reads = 1092983 mem = 536878626 peak = 1031585792
[M::process] read 715624 sequences (100000014 bp) in 2.633 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304398, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (184.06, 70.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715712 sequences (100000256 bp) in 2.069 CPU sec, 2.074 real sec...
[M::mem_process_seqs] Processed 715952 reads in 192.624 CPU sec, 6.171 real sec
[M::mem_process_seqs] Processed 715786 reads in 112.266 CPU sec, 3.563 real sec
sorting block 9, #reads = 1092491 mem = 536881296 peak = 1031585792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304362, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (184.02, 69.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715386 sequences (100000038 bp) in 2.733 CPU sec, 2.806 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304200, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (188.67, 75.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715270 sequences (100000223 bp) in 2.067 CPU sec, 2.070 real sec...
[M::mem_process_seqs] Processed 715624 reads in 193.678 CPU sec, 6.241 real sec
[M::mem_process_seqs] Processed 715712 reads in 112.464 CPU sec, 3.654 real sec
sorting block 10, #reads = 1093009 mem = 536876950 peak = 1031585792
[M::process] read 715388 sequences (100000287 bp) in 2.987 CPU sec, 3.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304321, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (194.30, 83.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 55, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (234.00, 287.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715270 reads in 101.548 CPU sec, 3.208 real sec
sorting block 11, #reads = 1092223 mem = 536878304 peak = 1031585792
[M::process] read 714484 sequences (100000174 bp) in 2.317 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304269, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.24, 78.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304500, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.37, 79.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715386 reads in 288.106 CPU sec, 9.230 real sec
[M::mem_process_seqs] Processed 715388 reads in 117.038 CPU sec, 3.720 real sec
[M::process] read 715520 sequences (100000034 bp) in 2.498 CPU sec, 2.528 real sec...
sorting block 12, #reads = 1092168 mem = 536879813 peak = 1031585792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303768, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (201.10, 93.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716370 sequences (100000211 bp) in 3.072 CPU sec, 3.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304479, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (191.74, 80.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714484 reads in 187.340 CPU sec, 5.992 real sec
[M::mem_process_seqs] Processed 715520 reads in 107.777 CPU sec, 3.402 real sec
sorting block 13, #reads = 1092180 mem = 536879621 peak = 1031585792
[M::process] read 716022 sequences (100000212 bp) in 2.573 CPU sec, 2.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304791, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 163, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 351)
[M::mem_pestat] mean and std.dev: (176.89, 61.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1091693 mem = 536878512 peak = 1064292352
[M::process] read 716104 sequences (100000006 bp) in 2.876 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304279, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (185.92, 72.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716370 reads in 191.444 CPU sec, 6.124 real sec
[M::mem_process_seqs] Processed 716022 reads in 111.071 CPU sec, 3.502 real sec
[M::process] read 715902 sequences (100000145 bp) in 2.700 CPU sec, 2.755 real sec...
sorting block 15, #reads = 1092756 mem = 536879854 peak = 1064292352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304295, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.79, 71.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715732 sequences (100000049 bp) in 3.145 CPU sec, 3.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304312, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (185.55, 71.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716104 reads in 189.570 CPU sec, 6.074 real sec
[M::mem_process_seqs] Processed 715902 reads in 109.838 CPU sec, 3.467 real sec
sorting block 16, #reads = 1092700 mem = 536877332 peak = 1064292352
[M::process] read 715450 sequences (100000063 bp) in 3.074 CPU sec, 3.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304074, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.56, 74.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715344 sequences (100000007 bp) in 2.926 CPU sec, 3.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304239, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (189.30, 76.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715732 reads in 188.932 CPU sec, 6.053 real sec
[M::mem_process_seqs] Processed 715450 reads in 108.856 CPU sec, 3.442 real sec
sorting block 17, #reads = 1092038 mem = 536879211 peak = 1064292352
[M::process] read 715434 sequences (100000201 bp) in 3.178 CPU sec, 3.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304232, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (189.94, 77.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 18, #reads = 1092335 mem = 536879635 peak = 1064292352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304222, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (188.69, 76.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715214 sequences (100000180 bp) in 3.105 CPU sec, 3.259 real sec...
[M::mem_process_seqs] Processed 715344 reads in 181.649 CPU sec, 5.832 real sec
[M::mem_process_seqs] Processed 715434 reads in 101.239 CPU sec, 3.209 real sec
[M::process] read 715388 sequences (100000115 bp) in 3.409 CPU sec, 3.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303833, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.28, 79.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1092591 mem = 536877207 peak = 1064292352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304413, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (188.42, 75.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715214 reads in 193.238 CPU sec, 6.280 real sec
[M::process] read 715436 sequences (100000056 bp) in 3.026 CPU sec, 3.221 real sec...
[M::mem_process_seqs] Processed 715388 reads in 110.410 CPU sec, 3.539 real sec
sorting block 20, #reads = 1092008 mem = 536881768 peak = 1064292352
[M::process] read 715418 sequences (100000123 bp) in 3.279 CPU sec, 3.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304334, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (190.97, 79.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715436 reads in 103.139 CPU sec, 3.460 real sec
[M::process] read 715382 sequences (100000179 bp) in 2.851 CPU sec, 2.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303843, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (189.58, 77.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1092473 mem = 536879221 peak = 1064292352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304231, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (190.71, 78.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715418 reads in 193.724 CPU sec, 6.346 real sec
[M::process] read 715368 sequences (100000065 bp) in 3.335 CPU sec, 3.535 real sec...
[M::mem_process_seqs] Processed 715382 reads in 112.141 CPU sec, 3.606 real sec
sorting block 22, #reads = 1092175 mem = 536878787 peak = 1064292352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304115, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.28, 79.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715368 reads in 102.682 CPU sec, 3.438 real sec
[M::process] read 715294 sequences (100000052 bp) in 3.629 CPU sec, 3.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304489, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (192.27, 80.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715294 reads in 101.323 CPU sec, 3.414 real sec
[M::process] read 715412 sequences (100000042 bp) in 3.367 CPU sec, 3.537 real sec...
sorting block 23, #reads = 1092165 mem = 536879127 peak = 1064292352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304208, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (192.07, 80.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715412 reads in 101.395 CPU sec, 3.371 real sec
[M::process] read 715580 sequences (100000116 bp) in 3.620 CPU sec, 3.830 real sec...
sorting block 24, #reads = 1092486 mem = 536878553 peak = 1064292352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304892, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (187.51, 74.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715580 reads in 102.571 CPU sec, 3.421 real sec
[M::process] read 715438 sequences (100000006 bp) in 3.540 CPU sec, 3.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304371, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (191.32, 79.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1091965 mem = 536878126 peak = 1064292352
[M::mem_process_seqs] Processed 715438 reads in 100.173 CPU sec, 3.306 real sec
[M::process] read 715250 sequences (100000058 bp) in 3.600 CPU sec, 3.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304117, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.10, 85.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715250 reads in 103.988 CPU sec, 3.519 real sec
[M::process] read 715312 sequences (100000000 bp) in 3.232 CPU sec, 3.547 real sec...
sorting block 26, #reads = 1092762 mem = 536880903 peak = 1064292352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 303873, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.19, 79.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715312 reads in 100.386 CPU sec, 3.341 real sec
[M::process] read 715538 sequences (100000010 bp) in 3.260 CPU sec, 3.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304401, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.65, 70.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1092092 mem = 536880300 peak = 1064292352
[M::process] read 715234 sequences (100000071 bp) in 2.984 CPU sec, 3.280 real sec...
[M::mem_process_seqs] Processed 715538 reads in 101.319 CPU sec, 3.381 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304409, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (188.19, 75.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716780 sequences (100000262 bp) in 2.814 CPU sec, 3.143 real sec...
[M::mem_process_seqs] Processed 715234 reads in 98.920 CPU sec, 3.308 real sec
sorting block 28, #reads = 1091898 mem = 536877131 peak = 1064292352
[M::process] read 716232 sequences (100000246 bp) in 2.729 CPU sec, 3.110 real sec...
[M::process] read 716182 sequences (100000158 bp) in 2.665 CPU sec, 2.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 304088, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 355)
[M::mem_pestat] mean and std.dev: (178.21, 63.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 432)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716232 reads in 101.770 CPU sec, 3.254 real sec
sorting block 29, #reads = 1091986 mem = 536878924 peak = 1070219264
[M::process] read 715886 sequences (100000036 bp) in 2.735 CPU sec, 2.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304976, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 330)
[M::mem_pestat] mean and std.dev: (169.94, 53.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 391)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304388, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (183.92, 70.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 465)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715618 sequences (100000094 bp) in 2.330 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304265, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (183.57, 69.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 53, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (73.86, 62.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 716780 reads in 377.315 CPU sec, 12.087 real sec
[M::mem_process_seqs] Processed 716182 reads in 210.107 CPU sec, 6.719 real sec
[M::mem_process_seqs] Processed 715886 reads in 120.423 CPU sec, 3.864 real sec
sorting block 30, #reads = 1092976 mem = 536878868 peak = 1070219264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304281, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (186.87, 73.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715600 sequences (100000135 bp) in 3.183 CPU sec, 3.340 real sec...
[M::mem_process_seqs] Processed 715618 reads in 101.305 CPU sec, 3.442 real sec
sorting block 31, #reads = 1092188 mem = 536878674 peak = 1102520320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304585, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.28, 78.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715720 sequences (100000009 bp) in 2.971 CPU sec, 3.186 real sec...
[M::mem_process_seqs] Processed 715600 reads in 102.298 CPU sec, 3.521 real sec
sorting block 32, #reads = 1092914 mem = 536877660 peak = 1102520320
[M::process] read 715290 sequences (100000163 bp) in 2.743 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304357, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (188.69, 75.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715288 sequences (100000164 bp) in 2.725 CPU sec, 2.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304188, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (192.25, 80.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715720 reads in 193.831 CPU sec, 6.339 real sec
[M::mem_process_seqs] Processed 715290 reads in 112.011 CPU sec, 3.654 real sec
[M::process] read 715230 sequences (100000193 bp) in 2.784 CPU sec, 2.963 real sec...
sorting block 33, #reads = 1092779 mem = 536879681 peak = 1102520320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304396, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (192.59, 81.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715390 sequences (100000230 bp) in 2.744 CPU sec, 3.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303789, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (193.28, 82.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715288 reads in 187.532 CPU sec, 6.109 real sec
[M::mem_process_seqs] Processed 715230 reads in 109.428 CPU sec, 3.521 real sec
sorting block 34, #reads = 1091979 mem = 536878684 peak = 1102520320
[M::process] read 715376 sequences (100000145 bp) in 2.858 CPU sec, 3.306 real sec...
sorting block 35, #reads = 1092065 mem = 536877056 peak = 1102520320
[M::process] read 715102 sequences (100000099 bp) in 2.792 CPU sec, 3.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303836, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (195.74, 86.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715376 reads in 103.649 CPU sec, 3.361 real sec
[M::process] read 715054 sequences (100000191 bp) in 2.811 CPU sec, 3.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304088, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.29, 85.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 303839, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.30, 88.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715390 reads in 290.490 CPU sec, 9.500 real sec
[M::mem_process_seqs] Processed 715102 reads in 120.154 CPU sec, 3.934 real sec
sorting block 36, #reads = 1091564 mem = 536880785 peak = 1102520320
[M::process] read 714962 sequences (100000177 bp) in 2.724 CPU sec, 2.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304308, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (200.01, 91.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1091910 mem = 536879242 peak = 1106694144
[M::process] read 714610 sequences (100000083 bp) in 2.792 CPU sec, 3.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303673, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (200.67, 92.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715054 reads in 192.508 CPU sec, 6.271 real sec
[M::mem_process_seqs] Processed 714962 reads in 111.265 CPU sec, 3.611 real sec
[M::process] read 714844 sequences (100000189 bp) in 2.827 CPU sec, 3.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303221, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (205.33, 99.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1091669 mem = 536877819 peak = 1106694144
[M::process] read 714808 sequences (100000027 bp) in 2.874 CPU sec, 3.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303959, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (201.96, 95.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714610 reads in 193.849 CPU sec, 6.324 real sec
[M::mem_process_seqs] Processed 714844 reads in 110.906 CPU sec, 3.601 real sec
sorting block 39, #reads = 1091561 mem = 536877617 peak = 1106694144
[M::process] read 714692 sequences (100000013 bp) in 2.754 CPU sec, 2.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303679, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (203.83, 97.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714938 sequences (100000062 bp) in 2.795 CPU sec, 3.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303357, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (204.25, 97.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714808 reads in 193.217 CPU sec, 6.317 real sec
[M::mem_process_seqs] Processed 714692 reads in 111.097 CPU sec, 3.609 real sec
sorting block 40, #reads = 1091834 mem = 536881059 peak = 1118437376
[M::process] read 715394 sequences (100000046 bp) in 2.650 CPU sec, 2.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303592, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (203.12, 96.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1091410 mem = 536878928 peak = 1123581952
[M::process] read 715736 sequences (100000081 bp) in 3.040 CPU sec, 3.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304395, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (191.15, 79.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714938 reads in 192.077 CPU sec, 6.260 real sec
[M::mem_process_seqs] Processed 715394 reads in 111.281 CPU sec, 3.587 real sec
[M::process] read 716118 sequences (100000236 bp) in 2.784 CPU sec, 2.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304699, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.42, 70.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 38, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (83.75, 100.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 42, #reads = 1091539 mem = 536882934 peak = 1123581952
[M::process] read 716188 sequences (100000132 bp) in 2.873 CPU sec, 3.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304431, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 346)
[M::mem_pestat] mean and std.dev: (175.40, 59.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 415)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715736 reads in 188.681 CPU sec, 6.139 real sec
[M::mem_process_seqs] Processed 716118 reads in 110.256 CPU sec, 3.558 real sec
sorting block 43, #reads = 1092553 mem = 536880641 peak = 1123581952
[M::process] read 715840 sequences (100000189 bp) in 2.771 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304255, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 340)
[M::mem_pestat] mean and std.dev: (173.72, 57.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715768 sequences (100000285 bp) in 3.092 CPU sec, 3.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304399, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (183.34, 69.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 20, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259)
[M::mem_pestat] mean and std.dev: (36.40, 51.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 344)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 716188 reads in 189.482 CPU sec, 6.149 real sec
[M::mem_process_seqs] Processed 715840 reads in 110.706 CPU sec, 3.570 real sec
sorting block 44, #reads = 1092391 mem = 536879890 peak = 1123581952
[M::process] read 715806 sequences (100000212 bp) in 3.025 CPU sec, 3.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304070, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (185.22, 71.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1092754 mem = 536878057 peak = 1151492096
[M::process] read 715652 sequences (100000214 bp) in 2.985 CPU sec, 3.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304372, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (186.10, 72.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715768 reads in 191.249 CPU sec, 6.198 real sec
[M::mem_process_seqs] Processed 715806 reads in 110.976 CPU sec, 3.557 real sec
[M::process] read 715648 sequences (100000096 bp) in 2.702 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303862, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (187.25, 73.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 49, 115)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343)
[M::mem_pestat] mean and std.dev: (45.00, 51.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 457)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 46, #reads = 1092475 mem = 536879285 peak = 1151492096
[M::process] read 715454 sequences (100000145 bp) in 3.031 CPU sec, 3.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304218, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (189.40, 76.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 333, 617)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785)
[M::mem_pestat] mean and std.dev: (227.78, 240.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2369)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715652 reads in 192.930 CPU sec, 6.248 real sec
[M::mem_process_seqs] Processed 715648 reads in 111.621 CPU sec, 3.578 real sec
[M::process] read 715366 sequences (100000011 bp) in 2.761 CPU sec, 2.795 real sec...
sorting block 47, #reads = 1092231 mem = 536878768 peak = 1151492096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304036, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (190.82, 78.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715342 sequences (100000062 bp) in 2.814 CPU sec, 3.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303935, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (194.56, 84.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715454 reads in 191.774 CPU sec, 6.157 real sec
sorting block 48, #reads = 1092345 mem = 536878919 peak = 1151492096
[M::process] read 715010 sequences (100000206 bp) in 2.659 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304070, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.23, 85.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715342 reads in 102.704 CPU sec, 3.342 real sec
[M::process] read 715214 sequences (100000121 bp) in 2.708 CPU sec, 2.845 real sec...
sorting block 49, #reads = 1092116 mem = 536880065 peak = 1151492096
[M::mem_process_seqs] Processed 715366 reads in 284.157 CPU sec, 9.140 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303964, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (195.37, 85.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715122 sequences (100000247 bp) in 2.689 CPU sec, 3.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303933, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (194.08, 83.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715010 reads in 198.951 CPU sec, 6.400 real sec
[M::mem_process_seqs] Processed 715214 reads in 109.693 CPU sec, 3.479 real sec
sorting block 50, #reads = 1092108 mem = 536878751 peak = 1151492096
[M::process] read 714944 sequences (100000108 bp) in 2.751 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304354, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (194.45, 83.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714860 sequences (100000121 bp) in 2.786 CPU sec, 2.927 real sec...
sorting block 51, #reads = 1092266 mem = 536877774 peak = 1154330624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303652, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (198.89, 90.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715122 reads in 189.600 CPU sec, 6.085 real sec
[M::mem_process_seqs] Processed 714944 reads in 111.567 CPU sec, 3.558 real sec
[M::process] read 714730 sequences (100000040 bp) in 2.581 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303510, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (200.83, 93.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1091927 mem = 536877710 peak = 1154330624
[M::process] read 714420 sequences (100000145 bp) in 2.930 CPU sec, 3.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303724, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (205.21, 98.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714860 reads in 189.536 CPU sec, 6.135 real sec
[M::mem_process_seqs] Processed 714730 reads in 110.017 CPU sec, 3.498 real sec
[M::process] read 714292 sequences (100000022 bp) in 2.787 CPU sec, 2.821 real sec...
sorting block 53, #reads = 1091831 mem = 536879602 peak = 1154330624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303884, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566)
[M::mem_pestat] mean and std.dev: (207.55, 101.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714372 sequences (100000286 bp) in 2.858 CPU sec, 3.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303434, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (208.63, 102.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714420 reads in 188.515 CPU sec, 6.090 real sec
[M::mem_process_seqs] Processed 714292 reads in 109.149 CPU sec, 3.490 real sec
sorting block 54, #reads = 1091631 mem = 536879084 peak = 1154330624
[M::process] read 714462 sequences (100000069 bp) in 2.634 CPU sec, 2.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303496, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (207.99, 101.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714534 sequences (100000135 bp) in 2.989 CPU sec, 3.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303303, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (208.02, 102.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714372 reads in 193.024 CPU sec, 6.249 real sec
sorting block 55, #reads = 1091541 mem = 536878050 peak = 1154330624
[M::mem_process_seqs] Processed 714462 reads in 112.612 CPU sec, 3.598 real sec
[M::process] read 715236 sequences (100000165 bp) in 2.833 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303594, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (206.88, 101.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1091329 mem = 536877970 peak = 1154330624
[M::process] read 715356 sequences (100000260 bp) in 2.891 CPU sec, 3.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304087, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (195.62, 85.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714534 reads in 190.038 CPU sec, 6.147 real sec
[M::mem_process_seqs] Processed 715236 reads in 109.442 CPU sec, 3.478 real sec
[M::process] read 717080 sequences (100000266 bp) in 2.907 CPU sec, 3.050 real sec...
sorting block 57, #reads = 1091265 mem = 536879029 peak = 1154330624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304726, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (192.86, 81.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716502 sequences (100000188 bp) in 3.001 CPU sec, 3.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304197, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 309)
[M::mem_pestat] mean and std.dev: (162.16, 42.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 363)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715356 reads in 191.339 CPU sec, 6.291 real sec
[M::mem_process_seqs] Processed 717080 reads in 113.574 CPU sec, 3.745 real sec
sorting block 58, #reads = 1092213 mem = 536879323 peak = 1154330624
[M::process] read 716354 sequences (100000058 bp) in 3.255 CPU sec, 3.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304463, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 327)
[M::mem_pestat] mean and std.dev: (168.41, 51.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 387)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304755, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 342)
[M::mem_pestat] mean and std.dev: (173.34, 57.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 407)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716600 sequences (100000046 bp) in 2.934 CPU sec, 3.110 real sec...
[M::mem_process_seqs] Processed 716502 reads in 193.141 CPU sec, 6.243 real sec
[M::mem_process_seqs] Processed 716354 reads in 112.525 CPU sec, 3.600 real sec
sorting block 59, #reads = 1092835 mem = 536878883 peak = 1154330624
[M::process] read 716156 sequences (100000104 bp) in 3.046 CPU sec, 3.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304068, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 342)
[M::mem_pestat] mean and std.dev: (173.34, 57.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 407)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1092638 mem = 536877447 peak = 1154330624
[M::process] read 716134 sequences (100000108 bp) in 2.927 CPU sec, 3.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303610, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 355)
[M::mem_pestat] mean and std.dev: (177.70, 62.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 429)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716600 reads in 195.577 CPU sec, 6.340 real sec
[M::mem_process_seqs] Processed 716156 reads in 114.834 CPU sec, 3.708 real sec
[M::process] read 716238 sequences (100000139 bp) in 2.662 CPU sec, 2.722 real sec...
sorting block 61, #reads = 1092836 mem = 536877914 peak = 1154330624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304165, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 364)
[M::mem_pestat] mean and std.dev: (179.96, 65.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 443)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716262 sequences (100000006 bp) in 2.766 CPU sec, 3.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304282, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 348)
[M::mem_pestat] mean and std.dev: (175.76, 60.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716134 reads in 194.882 CPU sec, 6.284 real sec
[M::mem_process_seqs] Processed 716238 reads in 113.704 CPU sec, 3.638 real sec
sorting block 62, #reads = 1092736 mem = 536878747 peak = 1154330624
[M::process] read 716220 sequences (100000095 bp) in 2.793 CPU sec, 2.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304196, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 327)
[M::mem_pestat] mean and std.dev: (168.73, 51.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 387)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716190 sequences (100000007 bp) in 2.849 CPU sec, 3.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304359, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 337)
[M::mem_pestat] mean and std.dev: (172.61, 56.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 400)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716262 reads in 192.055 CPU sec, 6.191 real sec
[M::mem_process_seqs] Processed 716220 reads in 112.141 CPU sec, 3.576 real sec
sorting block 63, #reads = 1092906 mem = 536880255 peak = 1163673600
[M::process] read 716300 sequences (100000196 bp) in 2.607 CPU sec, 2.756 real sec...
sorting block 64, #reads = 1092911 mem = 536879721 peak = 1163673600
[M::process] read 716222 sequences (100000273 bp) in 3.331 CPU sec, 3.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304812, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 334)
[M::mem_pestat] mean and std.dev: (171.43, 54.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716300 reads in 104.571 CPU sec, 3.349 real sec
[M::process] read 716358 sequences (100000223 bp) in 2.963 CPU sec, 3.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 305059, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 321)
[M::mem_pestat] mean and std.dev: (167.10, 48.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 379)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304280, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 333)
[M::mem_pestat] mean and std.dev: (171.02, 54.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716190 reads in 294.043 CPU sec, 9.513 real sec
[M::mem_process_seqs] Processed 716222 reads in 123.005 CPU sec, 3.952 real sec
sorting block 65, #reads = 1093053 mem = 536879081 peak = 1163673600
[M::process] read 716274 sequences (100000191 bp) in 3.190 CPU sec, 3.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304832, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 327)
[M::mem_pestat] mean and std.dev: (169.01, 51.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 387)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716358 reads in 101.946 CPU sec, 3.461 real sec
sorting block 66, #reads = 1093203 mem = 536878291 peak = 1163673600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304497, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 336)
[M::mem_pestat] mean and std.dev: (171.87, 55.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 399)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715898 sequences (100000081 bp) in 3.294 CPU sec, 3.576 real sec...
[M::mem_process_seqs] Processed 716274 reads in 102.153 CPU sec, 3.423 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304493, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 340)
[M::mem_pestat] mean and std.dev: (173.59, 57.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715946 sequences (100000042 bp) in 2.954 CPU sec, 3.233 real sec...
[M::mem_process_seqs] Processed 715898 reads in 101.277 CPU sec, 3.415 real sec
sorting block 67, #reads = 1093056 mem = 536878497 peak = 1163673600
[M::process] read 715980 sequences (100000005 bp) in 3.044 CPU sec, 3.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304675, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 343)
[M::mem_pestat] mean and std.dev: (174.38, 58.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 408)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304776, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 349)
[M::mem_pestat] mean and std.dev: (176.44, 60.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 420)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715946 reads in 191.513 CPU sec, 6.280 real sec
sorting block 68, #reads = 1092757 mem = 536877051 peak = 1163673600
[M::process] read 715734 sequences (100000241 bp) in 3.154 CPU sec, 3.399 real sec...
[M::mem_process_seqs] Processed 715980 reads in 111.939 CPU sec, 3.650 real sec
[M::process] read 715492 sequences (100000046 bp) in 2.923 CPU sec, 3.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304633, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373)
[M::mem_pestat] mean and std.dev: (182.40, 68.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 455)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1091539 mem = 536879069 peak = 1165479936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304455, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (185.55, 71.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 14, 22)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 62)
[M::mem_pestat] mean and std.dev: (10.38, 8.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 82)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715734 reads in 195.753 CPU sec, 6.426 real sec
[M::process] read 715418 sequences (100000287 bp) in 3.052 CPU sec, 3.450 real sec...
[M::mem_process_seqs] Processed 715492 reads in 114.497 CPU sec, 3.752 real sec
sorting block 70, #reads = 1093010 mem = 536877919 peak = 1165479936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304289, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (186.24, 72.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715574 sequences (100000063 bp) in 3.119 CPU sec, 3.292 real sec...
[M::mem_process_seqs] Processed 715418 reads in 100.572 CPU sec, 3.408 real sec
[M::process] read 715148 sequences (100000089 bp) in 3.092 CPU sec, 3.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304135, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (181.71, 67.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1092514 mem = 536879574 peak = 1165479936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303995, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (197.16, 88.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 628)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716890 sequences (100000017 bp) in 3.033 CPU sec, 3.226 real sec...
[M::mem_process_seqs] Processed 715574 reads in 191.490 CPU sec, 6.246 real sec
[M::mem_process_seqs] Processed 715148 reads in 110.499 CPU sec, 3.617 real sec
sorting block 72, #reads = 1092175 mem = 536881370 peak = 1165479936
[M::process] read 716752 sequences (100000113 bp) in 2.999 CPU sec, 3.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304800, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 312)
[M::mem_pestat] mean and std.dev: (163.34, 43.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716560 sequences (100000208 bp) in 3.051 CPU sec, 3.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304532, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 318)
[M::mem_pestat] mean and std.dev: (165.31, 46.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 375)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716890 reads in 197.073 CPU sec, 6.384 real sec
[M::mem_process_seqs] Processed 716752 reads in 115.838 CPU sec, 3.717 real sec
sorting block 73, #reads = 1092111 mem = 536877731 peak = 1165479936
[M::process] read 716546 sequences (100000044 bp) in 2.690 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304018, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 333)
[M::mem_pestat] mean and std.dev: (170.59, 54.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1092741 mem = 536880567 peak = 1165479936
[M::process] read 716168 sequences (100000126 bp) in 2.880 CPU sec, 3.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 304457, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 345)
[M::mem_pestat] mean and std.dev: (174.48, 59.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 411)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716560 reads in 193.739 CPU sec, 6.247 real sec
[M::mem_process_seqs] Processed 716546 reads in 112.367 CPU sec, 3.611 real sec
[M::process] read 716256 sequences (100000011 bp) in 2.556 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304136, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 340)
[M::mem_pestat] mean and std.dev: (173.09, 57.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1092936 mem = 536882088 peak = 1165479936
[M::process] read 715988 sequences (100000113 bp) in 3.277 CPU sec, 3.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304254, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (178.44, 63.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716168 reads in 194.651 CPU sec, 6.334 real sec
[M::mem_process_seqs] Processed 716256 reads in 114.609 CPU sec, 3.694 real sec
[M::process] read 715980 sequences (100000116 bp) in 2.818 CPU sec, 2.868 real sec...
sorting block 76, #reads = 1092714 mem = 536877291 peak = 1165479936
[M::process] read 715832 sequences (100000276 bp) in 3.005 CPU sec, 3.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304322, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (178.57, 63.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715980 reads in 105.276 CPU sec, 3.357 real sec
sorting block 77, #reads = 1092734 mem = 536877981 peak = 1165479936
[M::process] read 715648 sequences (100000245 bp) in 2.890 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304507, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (181.40, 67.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304258, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (185.57, 72.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715988 reads in 297.182 CPU sec, 9.566 real sec
[M::mem_process_seqs] Processed 715832 reads in 125.255 CPU sec, 4.060 real sec
[M::process] read 715630 sequences (100000165 bp) in 3.150 CPU sec, 3.397 real sec...
sorting block 78, #reads = 1092485 mem = 536879111 peak = 1165479936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 303912, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 361)
[M::mem_pestat] mean and std.dev: (179.76, 64.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 439)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 112, 652)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934)
[M::mem_pestat] mean and std.dev: (132.62, 210.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2575)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 303997, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (183.94, 70.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715872 sequences (100000067 bp) in 3.427 CPU sec, 3.742 real sec...
[M::mem_process_seqs] Processed 715648 reads in 193.128 CPU sec, 6.288 real sec
[M::mem_process_seqs] Processed 715630 reads in 113.424 CPU sec, 3.645 real sec
sorting block 79, #reads = 1092364 mem = 536879486 peak = 1165479936
[M::process] read 715652 sequences (100000105 bp) in 2.875 CPU sec, 3.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304319, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (182.57, 68.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 457)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715832 sequences (100000033 bp) in 2.975 CPU sec, 3.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304154, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 364)
[M::mem_pestat] mean and std.dev: (180.03, 65.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 442)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 207, 987)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2879)
[M::mem_pestat] mean and std.dev: (766.10, 1041.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4931)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715872 reads in 195.529 CPU sec, 6.312 real sec
sorting block 80, #reads = 1092262 mem = 536876474 peak = 1165479936
[M::mem_process_seqs] Processed 715652 reads in 114.146 CPU sec, 3.639 real sec
[M::process] read 715608 sequences (100000192 bp) in 2.851 CPU sec, 3.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304208, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (182.38, 68.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 456)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 81, #reads = 1092647 mem = 536881665 peak = 1173745664
[M::process] read 715648 sequences (100000187 bp) in 2.980 CPU sec, 3.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304386, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.19, 70.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715832 reads in 196.607 CPU sec, 6.381 real sec
[M::mem_process_seqs] Processed 715608 reads in 114.109 CPU sec, 3.674 real sec
[M::process] read 715842 sequences (100000247 bp) in 2.429 CPU sec, 2.561 real sec...
sorting block 82, #reads = 1092758 mem = 536879510 peak = 1173745664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304470, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.10, 70.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715854 sequences (100000123 bp) in 2.930 CPU sec, 3.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304215, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (181.40, 67.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715648 reads in 194.498 CPU sec, 6.338 real sec
sorting block 83, #reads = 1092974 mem = 536878622 peak = 1173745664
[M::process] read 715358 sequences (100000123 bp) in 3.003 CPU sec, 3.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304252, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367)
[M::mem_pestat] mean and std.dev: (180.69, 66.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 96, 96)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236)
[M::mem_pestat] mean and std.dev: (62.80, 35.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 306)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715854 reads in 104.652 CPU sec, 3.367 real sec
[M::process] read 715540 sequences (100000109 bp) in 2.896 CPU sec, 2.973 real sec...
[M::mem_process_seqs] Processed 715842 reads in 295.405 CPU sec, 9.498 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303690, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (185.96, 72.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 53, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681)
[M::mem_pestat] mean and std.dev: (253.50, 358.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2235)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 84, #reads = 1092636 mem = 536878900 peak = 1173745664
[M::process] read 715904 sequences (100000029 bp) in 2.891 CPU sec, 3.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304179, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (184.86, 71.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715358 reads in 205.885 CPU sec, 6.586 real sec
[M::mem_process_seqs] Processed 715540 reads in 113.372 CPU sec, 3.624 real sec
sorting block 85, #reads = 1092232 mem = 536881321 peak = 1173745664
[M::process] read 716044 sequences (100000115 bp) in 2.915 CPU sec, 3.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304949, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 352)
[M::mem_pestat] mean and std.dev: (176.86, 61.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 717036 sequences (100000069 bp) in 2.784 CPU sec, 3.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304231, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 343)
[M::mem_pestat] mean and std.dev: (173.77, 58.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 408)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715904 reads in 188.853 CPU sec, 6.122 real sec
[M::mem_process_seqs] Processed 716044 reads in 111.495 CPU sec, 3.557 real sec
sorting block 86, #reads = 1092209 mem = 536879124 peak = 1173745664
[M::process] read 716484 sequences (100000070 bp) in 2.823 CPU sec, 3.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304960, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 303)
[M::mem_pestat] mean and std.dev: (160.37, 39.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 87, #reads = 1092583 mem = 536877288 peak = 1173745664
[M::process] read 716464 sequences (100000026 bp) in 2.806 CPU sec, 3.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 305069, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 318)
[M::mem_pestat] mean and std.dev: (165.51, 46.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 375)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 717036 reads in 193.447 CPU sec, 6.309 real sec
[M::mem_process_seqs] Processed 716484 reads in 112.913 CPU sec, 3.677 real sec
[M::process] read 716284 sequences (100000103 bp) in 2.835 CPU sec, 2.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304557, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 327)
[M::mem_pestat] mean and std.dev: (168.60, 51.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 387)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 88, #reads = 1093144 mem = 536877130 peak = 1173745664
[M::process] read 716392 sequences (100000078 bp) in 3.130 CPU sec, 3.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304057, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 339)
[M::mem_pestat] mean and std.dev: (172.73, 56.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 403)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716464 reads in 196.914 CPU sec, 6.424 real sec
[M::mem_process_seqs] Processed 716284 reads in 116.409 CPU sec, 3.755 real sec
sorting block 89, #reads = 1093297 mem = 536878827 peak = 1173745664
[M::process] read 716212 sequences (100000182 bp) in 2.840 CPU sec, 2.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304321, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 163, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 357)
[M::mem_pestat] mean and std.dev: (177.96, 63.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 432)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715902 sequences (100000228 bp) in 2.970 CPU sec, 3.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303964, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367)
[M::mem_pestat] mean and std.dev: (180.58, 66.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716392 reads in 195.954 CPU sec, 6.354 real sec
[M::mem_process_seqs] Processed 716212 reads in 115.495 CPU sec, 3.681 real sec
sorting block 90, #reads = 1091430 mem = 536879180 peak = 1173745664
[M::process] read 715948 sequences (100000205 bp) in 2.759 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303498, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (183.37, 69.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 91, #reads = 1092947 mem = 536879557 peak = 1173745664
[M::process] read 715620 sequences (100000181 bp) in 3.005 CPU sec, 3.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304030, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (181.26, 67.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715902 reads in 194.955 CPU sec, 6.255 real sec
[M::mem_process_seqs] Processed 715948 reads in 112.701 CPU sec, 3.563 real sec
[M::process] read 715890 sequences (100000065 bp) in 2.672 CPU sec, 2.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304008, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (186.10, 72.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 92, #reads = 1092300 mem = 536879246 peak = 1173745664
[M::process] read 715442 sequences (100000055 bp) in 3.077 CPU sec, 3.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304434, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.48, 70.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715620 reads in 196.571 CPU sec, 6.354 real sec
[M::mem_process_seqs] Processed 715890 reads in 115.626 CPU sec, 3.678 real sec
[M::process] read 715586 sequences (100000062 bp) in 2.846 CPU sec, 2.883 real sec...
sorting block 93, #reads = 1092529 mem = 536881126 peak = 1173745664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303949, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (188.04, 75.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715594 sequences (100000267 bp) in 2.977 CPU sec, 3.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303810, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (188.67, 76.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715442 reads in 196.663 CPU sec, 6.353 real sec
[M::mem_process_seqs] Processed 715586 reads in 114.366 CPU sec, 3.668 real sec
sorting block 94, #reads = 1092486 mem = 536878882 peak = 1183449088
[M::process] read 715330 sequences (100000232 bp) in 3.301 CPU sec, 3.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304016, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (187.68, 75.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 95, #reads = 1092215 mem = 536881887 peak = 1184804864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303970, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (188.99, 76.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715226 sequences (100000039 bp) in 3.201 CPU sec, 3.545 real sec...
[M::mem_process_seqs] Processed 715594 reads in 197.015 CPU sec, 6.421 real sec
[M::mem_process_seqs] Processed 715330 reads in 114.412 CPU sec, 3.723 real sec
[M::process] read 715062 sequences (100000075 bp) in 2.860 CPU sec, 2.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303529, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (196.48, 87.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 96, #reads = 1092618 mem = 536880979 peak = 1184804864
[M::process] read 715180 sequences (100000097 bp) in 3.043 CPU sec, 3.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303539, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (200.62, 93.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 244, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220)
[M::mem_pestat] mean and std.dev: (213.90, 223.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1623)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715226 reads in 196.712 CPU sec, 6.349 real sec
[M::mem_process_seqs] Processed 715062 reads in 113.620 CPU sec, 3.624 real sec
[M::process] read 715272 sequences (100000256 bp) in 2.836 CPU sec, 2.935 real sec...
sorting block 97, #reads = 1092263 mem = 536879027 peak = 1184804864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303756, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (195.91, 87.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 303594, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (192.69, 82.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 20, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525)
[M::mem_pestat] mean and std.dev: (192.33, 362.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2032)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715144 sequences (100000172 bp) in 3.092 CPU sec, 3.752 real sec...
[M::mem_process_seqs] Processed 715180 reads in 198.377 CPU sec, 6.467 real sec
[M::mem_process_seqs] Processed 715272 reads in 115.545 CPU sec, 3.767 real sec
sorting block 98, #reads = 1091906 mem = 536877634 peak = 1184804864
[M::process] read 715330 sequences (100000131 bp) in 2.800 CPU sec, 2.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303406, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (196.11, 87.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 99, #reads = 1092165 mem = 536878859 peak = 1195155456
[M::process] read 715780 sequences (100000176 bp) in 2.998 CPU sec, 3.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304412, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (193.34, 83.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715144 reads in 193.747 CPU sec, 6.291 real sec
[M::mem_process_seqs] Processed 715330 reads in 111.167 CPU sec, 3.564 real sec
[M::process] read 715996 sequences (100000069 bp) in 2.923 CPU sec, 3.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304392, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (183.35, 69.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 100, #reads = 1091913 mem = 536878027 peak = 1195155456
[M::process] read 717054 sequences (100000064 bp) in 3.073 CPU sec, 3.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304414, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 355)
[M::mem_pestat] mean and std.dev: (177.17, 62.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715780 reads in 191.689 CPU sec, 6.194 real sec
[M::mem_process_seqs] Processed 715996 reads in 113.156 CPU sec, 3.614 real sec
sorting block 101, #reads = 1092359 mem = 536881009 peak = 1195155456
[M::process] read 716578 sequences (100000160 bp) in 2.818 CPU sec, 2.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304635, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 303)
[M::mem_pestat] mean and std.dev: (160.39, 39.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716332 sequences (100000137 bp) in 2.684 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 305299, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 324)
[M::mem_pestat] mean and std.dev: (167.52, 49.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 383)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 717054 reads in 194.574 CPU sec, 6.239 real sec
[M::mem_process_seqs] Processed 716578 reads in 112.723 CPU sec, 3.605 real sec
sorting block 102, #reads = 1092561 mem = 536877060 peak = 1195155456
[M::process] read 716246 sequences (100000255 bp) in 2.677 CPU sec, 2.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304110, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 339)
[M::mem_pestat] mean and std.dev: (172.54, 56.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 403)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 103, #reads = 1093513 mem = 536878319 peak = 1195155456
[M::process] read 715746 sequences (100000236 bp) in 2.811 CPU sec, 3.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304765, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 352)
[M::mem_pestat] mean and std.dev: (176.90, 61.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716332 reads in 185.341 CPU sec, 6.015 real sec
[M::mem_process_seqs] Processed 716246 reads in 104.065 CPU sec, 3.319 real sec
[M::process] read 716044 sequences (100000082 bp) in 2.951 CPU sec, 3.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303890, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (182.44, 68.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 456)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1092672 mem = 536879987 peak = 1195155456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303986, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367)
[M::mem_pestat] mean and std.dev: (180.88, 66.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 448)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715892 sequences (100000075 bp) in 3.532 CPU sec, 3.605 real sec...
[M::mem_process_seqs] Processed 715746 reads in 186.886 CPU sec, 5.967 real sec
[M::mem_process_seqs] Processed 716044 reads in 108.072 CPU sec, 3.409 real sec
sorting block 105, #reads = 1092613 mem = 536878078 peak = 1195155456
[M::process] read 715750 sequences (100000220 bp) in 2.447 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303843, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (186.40, 73.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715604 sequences (100000286 bp) in 2.846 CPU sec, 2.907 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303995, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (185.19, 71.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715892 reads in 180.446 CPU sec, 5.737 real sec
[M::mem_process_seqs] Processed 715750 reads in 102.735 CPU sec, 3.235 real sec
sorting block 106, #reads = 1092458 mem = 536879774 peak = 1195155456
[M::process] read 715550 sequences (100000151 bp) in 2.645 CPU sec, 2.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303777, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (188.90, 76.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715078 sequences (100000072 bp) in 3.006 CPU sec, 3.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303867, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.41, 74.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715604 reads in 196.966 CPU sec, 6.294 real sec
sorting block 107, #reads = 1092252 mem = 536880558 peak = 1195155456
[M::mem_process_seqs] Processed 715550 reads in 112.796 CPU sec, 3.565 real sec
[M::process] read 715008 sequences (100000098 bp) in 2.691 CPU sec, 2.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303634, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.01, 79.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 108, #reads = 1092480 mem = 536879306 peak = 1201954816
[M::process] read 715086 sequences (100000086 bp) in 3.005 CPU sec, 3.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303710, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (189.45, 76.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715078 reads in 193.452 CPU sec, 6.218 real sec
[M::mem_process_seqs] Processed 715008 reads in 112.445 CPU sec, 3.574 real sec
[M::process] read 715058 sequences (100000007 bp) in 2.462 CPU sec, 2.497 real sec...
sorting block 109, #reads = 1091981 mem = 536877734 peak = 1201954816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303252, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (194.14, 83.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714824 sequences (100000150 bp) in 3.211 CPU sec, 3.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303350, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (196.25, 86.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715086 reads in 193.229 CPU sec, 6.193 real sec
[M::mem_process_seqs] Processed 715058 reads in 112.625 CPU sec, 3.567 real sec
[M::process] read 715008 sequences (100000195 bp) in 2.801 CPU sec, 2.837 real sec...
sorting block 110, #reads = 1091638 mem = 536876827 peak = 1201954816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303280, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (199.79, 91.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714706 sequences (100000048 bp) in 3.061 CPU sec, 3.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303627, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (203.68, 97.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714824 reads in 195.382 CPU sec, 6.282 real sec
sorting block 111, #reads = 1091789 mem = 536879100 peak = 1213853696
[M::mem_process_seqs] Processed 715008 reads in 112.349 CPU sec, 3.555 real sec
[M::process] read 714694 sequences (100000071 bp) in 3.045 CPU sec, 3.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303373, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (206.03, 100.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 112, #reads = 1091904 mem = 536881532 peak = 1213853696
[M::process] read 714554 sequences (100000198 bp) in 3.166 CPU sec, 3.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303276, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (204.63, 98.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 28, 42)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 110)
[M::mem_pestat] mean and std.dev: (25.30, 21.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 144)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 714706 reads in 197.236 CPU sec, 6.363 real sec
[M::mem_process_seqs] Processed 714694 reads in 113.887 CPU sec, 3.622 real sec
[M::process] read 714632 sequences (100000126 bp) in 2.901 CPU sec, 2.994 real sec...
sorting block 113, #reads = 1091656 mem = 536878826 peak = 1213853696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303093, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (203.87, 97.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 3786, 9655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28877)
[M::mem_pestat] mean and std.dev: (4552.09, 4133.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38488)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715352 sequences (100000192 bp) in 3.073 CPU sec, 3.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303344, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (203.31, 96.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714554 reads in 198.376 CPU sec, 6.380 real sec
[M::mem_process_seqs] Processed 714632 reads in 114.353 CPU sec, 3.633 real sec
sorting block 114, #reads = 1091150 mem = 536876829 peak = 1213853696
[M::process] read 715624 sequences (100000133 bp) in 2.718 CPU sec, 2.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303876, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (189.42, 76.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 190676 sequences (26642896 bp) in 1.132 CPU sec, 1.264 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304311, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (184.61, 70.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715352 reads in 187.846 CPU sec, 6.114 real sec
[M::mem_process_seqs] Processed 715624 reads in 108.642 CPU sec, 3.547 real sec
sorting block 115, #reads = 1090837 mem = 536878604 peak = 1213853696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 80953, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (185.88, 72.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 190676 reads in 27.129 CPU sec, 0.938 real sec
sorting block 116, #reads = 1092302 mem = 536877866 peak = 1213853696
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 127575878
overall: 69874819072 mem 19633.297 user 569.842 sys 677.643 real
sorting block 117, #reads = 418011 mem = 205483064 peak = 1213853696
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.D0001483_22q.L004\tPU:L004\tSM:D0001483_22q\tLB:D0001483_22q\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.D0001483_22q_R1.fastq.gz 0001.D0001483_22q_R2.fastq.gz
[main] Real time: 677.685 sec; CPU: 20203.151 sec
uniq header count 1
merging 118 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 5366223177
output reads: 128222834
bam_mem_sort: 118 calls 135.769 user 2.563 sys 141.004 real
bam_write: 118 calls 91.866 user 6.212 sys 170.743 real
execute: 1 calls 47.788 user 2.492 sys 729.650 real
merge_files: 1 calls 47.731 user 2.479 sys 51.584 real
parse_chunk: 1879 calls 165.087 user 30.636 sys 231.608 real
read_chunk: 5083 calls 67.008 user 35.587 sys 619.596 real
sort_block: 1 calls 49.159 user 35.175 sys 592.761 real
write_chunk: 3202 calls 46.714 user 2.221 sys 49.963 real
overall: 2736263168 mem 2034.848 user 99.000 sys 729.673 real
ls: cannot access '*.cram': No such file or directory