This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o SH032_HG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2927.852 CPU sec, 124.999 real sec
[M::process] read 728094 sequences (100000073 bp) in 1.230 CPU sec, 1.230 real sec...
[M::process] read 727084 sequences (100000275 bp) in 2.062 CPU sec, 2.112 real sec...
[M::process] read 726700 sequences (100000226 bp) in 1.857 CPU sec, 1.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 307293, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 179, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (165.65, 46.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.31, 142.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 188, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365)
[M::mem_pestat] mean and std.dev: (179.30, 48.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 439)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726322 sequences (100000103 bp) in 1.658 CPU sec, 1.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306510, 9, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 144, 146)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (104, 174)
[M::mem_pestat] mean and std.dev: (144.89, 10.50)
[M::mem_pestat] low and high boundaries for proper pairs: (90, 188)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.65, 143.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 223, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 347)
[M::mem_pestat] mean and std.dev: (214.15, 37.35)
[M::mem_pestat] low and high boundaries for proper pairs: (59, 395)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306512, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 158, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 343)
[M::mem_pestat] mean and std.dev: (180.42, 52.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 409)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (279.64, 142.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728094 reads in 318.622 CPU sec, 10.446 real sec
[M::mem_process_seqs] Processed 726700 reads in 202.670 CPU sec, 6.772 real sec
[M::mem_process_seqs] Processed 727084 reads in 214.010 CPU sec, 7.182 real sec
sorting block 0, #reads = 1104246 mem = 536879457 peak = 584032256
[M::process] read 726114 sequences (100000077 bp) in 2.251 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306165, 10, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 162, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (181.12, 63.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 465)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.65, 142.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 55, 101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237)
[M::mem_pestat] mean and std.dev: (56.00, 28.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 305)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 725890 sequences (100000261 bp) in 2.171 CPU sec, 2.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306178, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 167, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (176.29, 60.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.46, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 172, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489)
[M::mem_pestat] mean and std.dev: (202.41, 67.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 599)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726322 reads in 197.077 CPU sec, 6.337 real sec
sorting block 1, #reads = 1103885 mem = 536879497 peak = 797941760
[M::process] read 725434 sequences (100000259 bp) in 1.601 CPU sec, 1.616 real sec...
[M::mem_process_seqs] Processed 726114 reads in 115.836 CPU sec, 3.719 real sec
sorting block 2, #reads = 1103312 mem = 536878833 peak = 858259456
[M::process] read 725628 sequences (100000154 bp) in 1.915 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306017, 9, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 179, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (209.72, 107.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.80, 142.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 207, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (189.86, 49.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726330 sequences (100000280 bp) in 1.139 CPU sec, 1.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306034, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 162, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (182.39, 78.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (283.40, 143.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 178, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 323)
[M::mem_pestat] mean and std.dev: (175.93, 44.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725890 reads in 193.835 CPU sec, 6.256 real sec
[M::mem_process_seqs] Processed 725434 reads in 113.101 CPU sec, 3.682 real sec
sorting block 3, #reads = 1102935 mem = 536878667 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306278, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 172, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (197.95, 71.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (284.27, 144.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 229, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (233.14, 93.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727464 sequences (100000175 bp) in 2.555 CPU sec, 2.856 real sec...
[M::process] read 727488 sequences (100000123 bp) in 1.600 CPU sec, 1.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305928, 10, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 172, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (184.71, 63.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.48, 142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (48, 78, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174)
[M::mem_pestat] mean and std.dev: (55.88, 22.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 216)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 183, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (223.64, 129.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725628 reads in 196.332 CPU sec, 6.312 real sec
[M::mem_process_seqs] Processed 726330 reads in 115.570 CPU sec, 3.718 real sec
sorting block 4, #reads = 1102516 mem = 536878453 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306791, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 167, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (197.93, 80.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 563)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.39, 142.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 204, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (223.63, 114.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727410 sequences (100000151 bp) in 2.230 CPU sec, 2.379 real sec...
[M::process] read 727138 sequences (100000087 bp) in 1.675 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306976, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 212, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (111, 311)
[M::mem_pestat] mean and std.dev: (204.06, 43.21)
[M::mem_pestat] low and high boundaries for proper pairs: (31, 377)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (276.62, 141.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 254, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (202.53, 74.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727464 reads in 201.924 CPU sec, 6.513 real sec
sorting block 5, #reads = 1102862 mem = 536877811 peak = 858259456
[M::mem_process_seqs] Processed 727488 reads in 120.019 CPU sec, 3.895 real sec
sorting block 6, #reads = 1104167 mem = 536879343 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306672, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 166, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (183.58, 92.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (276.92, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 211, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (221.20, 75.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725434 sequences (100000027 bp) in 2.426 CPU sec, 2.688 real sec...
[M::process] read 724432 sequences (100000208 bp) in 1.462 CPU sec, 1.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306584, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 249, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (249.79, 183.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.54, 141.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 200, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 341)
[M::mem_pestat] mean and std.dev: (174.62, 39.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 405)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727410 reads in 206.755 CPU sec, 6.683 real sec
[M::mem_process_seqs] Processed 727138 reads in 120.756 CPU sec, 3.911 real sec
sorting block 7, #reads = 1103967 mem = 536881982 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305693, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 209, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (209.46, 71.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (286.05, 145.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 237, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (199.46, 89.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723178 sequences (100000287 bp) in 2.810 CPU sec, 3.125 real sec...
[M::process] read 722774 sequences (100000056 bp) in 1.663 CPU sec, 1.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305085, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 209, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (210.09, 55.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (289.82, 147.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 187, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (193.80, 70.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725434 reads in 202.024 CPU sec, 6.490 real sec
[M::mem_process_seqs] Processed 724432 reads in 116.979 CPU sec, 3.756 real sec
sorting block 8, #reads = 1103849 mem = 536879097 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305167, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 185, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (200.50, 93.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 667)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 273, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (296.58, 150.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 251, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (233.40, 110.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726330 sequences (100000018 bp) in 2.400 CPU sec, 2.675 real sec...
[M::process] read 727616 sequences (100000058 bp) in 1.190 CPU sec, 1.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 303603, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 228, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (226.18, 92.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 276, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (298.87, 152.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 188, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (213.87, 90.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723178 reads in 200.055 CPU sec, 6.475 real sec
sorting block 9, #reads = 1101886 mem = 536879570 peak = 858259456
[M::process] read 726556 sequences (100000084 bp) in 1.791 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306178, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 213, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (195.85, 84.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (284.99, 146.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 237, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (223.79, 83.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726330 reads in 111.457 CPU sec, 3.761 real sec
sorting block 10, #reads = 1101357 mem = 536880356 peak = 858259456
[M::mem_process_seqs] Processed 722774 reads in 268.423 CPU sec, 8.833 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306628, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 170, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (207.45, 122.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 768)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.95, 141.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 162, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (172.53, 81.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727058 sequences (100000176 bp) in 2.931 CPU sec, 3.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306438, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 173, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (174.67, 50.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 256, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (279.51, 141.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 190, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (192.31, 69.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727616 reads in 207.935 CPU sec, 6.657 real sec
[M::process] read 726148 sequences (100000102 bp) in 1.807 CPU sec, 1.842 real sec...
[M::mem_process_seqs] Processed 726556 reads in 114.977 CPU sec, 3.667 real sec
sorting block 11, #reads = 1101491 mem = 536877694 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306957, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 170, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 364)
[M::mem_pestat] mean and std.dev: (166.69, 38.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 436)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (278.81, 141.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 124, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (131.25, 37.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 12, #reads = 1104183 mem = 536880539 peak = 858259456
[M::process] read 726326 sequences (100000096 bp) in 2.942 CPU sec, 3.232 real sec...
[M::process] read 725904 sequences (100000077 bp) in 1.699 CPU sec, 1.707 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306447, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 217, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (200.24, 70.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 258, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (281.13, 142.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727058 reads in 201.818 CPU sec, 6.615 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306741, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 221, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (83, 323)
[M::mem_pestat] mean and std.dev: (196.42, 48.20)
[M::mem_pestat] low and high boundaries for proper pairs: (4, 389)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.89, 143.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 192, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (206.65, 96.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726326 reads in 100.662 CPU sec, 3.230 real sec
[M::process] read 725744 sequences (100000247 bp) in 2.235 CPU sec, 2.275 real sec...
sorting block 13, #reads = 1103896 mem = 536879334 peak = 858259456
[M::mem_process_seqs] Processed 726148 reads in 272.877 CPU sec, 8.978 real sec
sorting block 14, #reads = 1103211 mem = 536878825 peak = 858259456
[M::process] read 725904 sequences (100000049 bp) in 2.834 CPU sec, 3.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306286, 5, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (282.83, 143.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 245, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 454)
[M::mem_pestat] mean and std.dev: (218.19, 66.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725744 reads in 102.965 CPU sec, 3.261 real sec
[M::process] read 726276 sequences (100000129 bp) in 1.677 CPU sec, 1.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306055, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 187, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (211.00, 75.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 715)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.78, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 182, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377)
[M::mem_pestat] mean and std.dev: (178.15, 42.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 305880, 8, 24)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 260, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (282.42, 143.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 190, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (201.21, 61.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725904 reads in 300.775 CPU sec, 9.604 real sec
[M::mem_process_seqs] Processed 725904 reads in 119.232 CPU sec, 3.826 real sec
sorting block 15, #reads = 1103084 mem = 536877846 peak = 858259456
[M::process] read 726436 sequences (100000234 bp) in 2.497 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306431, 3, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.81, 143.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 219, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420)
[M::mem_pestat] mean and std.dev: (196.06, 72.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1102888 mem = 536879152 peak = 858259456
[M::process] read 726336 sequences (100000277 bp) in 3.260 CPU sec, 3.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 306311, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 181, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (196.08, 68.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (280.39, 142.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 236, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (238.73, 94.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726276 reads in 195.267 CPU sec, 6.284 real sec
[M::process] read 726272 sequences (100000168 bp) in 2.209 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306625, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 168, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 296)
[M::mem_pestat] mean and std.dev: (159.06, 38.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 351)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (281.47, 143.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 167, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (168.28, 44.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 478)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725428 sequences (100000105 bp) in 1.372 CPU sec, 1.380 real sec...
[M::mem_process_seqs] Processed 726336 reads in 100.872 CPU sec, 3.215 real sec
sorting block 17, #reads = 1103344 mem = 536879720 peak = 858259456
[M::mem_process_seqs] Processed 726436 reads in 259.717 CPU sec, 8.305 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306063, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 219, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (243.41, 99.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.34, 143.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 149, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (185.00, 86.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1103888 mem = 536878444 peak = 858259456
[M::process] read 723990 sequences (100000103 bp) in 2.541 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306057, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (231.07, 99.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (286.14, 145.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 193, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453)
[M::mem_pestat] mean and std.dev: (181.14, 52.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 554)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726272 reads in 200.732 CPU sec, 6.424 real sec
[M::mem_process_seqs] Processed 725428 reads in 110.058 CPU sec, 3.510 real sec
[M::process] read 724050 sequences (100000236 bp) in 2.048 CPU sec, 2.106 real sec...
sorting block 19, #reads = 1103236 mem = 536880396 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305221, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 160, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (154.53, 69.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (291.86, 148.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 237, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 480)
[M::mem_pestat] mean and std.dev: (235.18, 66.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 574)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723346 sequences (100000132 bp) in 2.913 CPU sec, 3.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305233, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 213, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (186.67, 83.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (293.04, 149.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 183, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (206.45, 85.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723990 reads in 190.198 CPU sec, 6.113 real sec
[M::process] read 723264 sequences (100000033 bp) in 2.074 CPU sec, 2.095 real sec...
[M::mem_process_seqs] Processed 724050 reads in 111.391 CPU sec, 3.559 real sec
sorting block 20, #reads = 1101914 mem = 536881540 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305485, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 220, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (182.67, 58.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 272, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (294.44, 149.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 197, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (192.85, 85.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728220 sequences (100000210 bp) in 3.272 CPU sec, 3.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 304845, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 279, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (248.43, 88.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (292.86, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 191, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (184.89, 78.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723346 reads in 192.662 CPU sec, 6.193 real sec
[M::mem_process_seqs] Processed 723264 reads in 113.101 CPU sec, 3.601 real sec
sorting block 21, #reads = 1100778 mem = 536878372 peak = 858259456
[M::process] read 727294 sequences (100000020 bp) in 3.179 CPU sec, 3.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306727, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 186, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (183.77, 60.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (278.77, 144.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 227, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 399)
[M::mem_pestat] mean and std.dev: (214.13, 84.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 554)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1101003 mem = 536875615 peak = 858259456
[M::process] read 726594 sequences (100000195 bp) in 2.968 CPU sec, 3.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307453, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 159, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336)
[M::mem_pestat] mean and std.dev: (162.47, 59.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 404)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.72, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 211, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (201.54, 69.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728220 reads in 190.750 CPU sec, 6.147 real sec
[M::mem_process_seqs] Processed 727294 reads in 112.449 CPU sec, 3.597 real sec
[M::process] read 726718 sequences (100000027 bp) in 3.129 CPU sec, 3.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306817, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 132, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (166.69, 84.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (282.01, 144.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 165, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 328)
[M::mem_pestat] mean and std.dev: (159.69, 46.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 393)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 23, #reads = 1104167 mem = 536878632 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306202, 0, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 159, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (172.60, 50.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (280.89, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 195, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (200.16, 65.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726594 reads in 189.843 CPU sec, 6.153 real sec
[M::mem_process_seqs] Processed 726718 reads in 111.058 CPU sec, 3.587 real sec
[M::process] read 725946 sequences (100000117 bp) in 4.188 CPU sec, 4.756 real sec...
sorting block 24, #reads = 1104085 mem = 536875927 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306686, 6, 24)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.00, 144.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 229, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (203.54, 81.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725946 reads in 103.585 CPU sec, 3.458 real sec
[M::process] read 726004 sequences (100000087 bp) in 4.279 CPU sec, 4.875 real sec...
sorting block 25, #reads = 1103453 mem = 536877851 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306418, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 172, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (155.75, 59.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.40, 145.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 241, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (239.58, 100.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726004 reads in 99.935 CPU sec, 3.300 real sec
[M::process] read 725596 sequences (100000001 bp) in 3.328 CPU sec, 3.833 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305910, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 182, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (190.92, 64.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 558)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (284.29, 143.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 152, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (167.33, 71.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725596 reads in 95.571 CPU sec, 3.151 real sec
sorting block 26, #reads = 1102968 mem = 536879496 peak = 858259456
[M::process] read 725574 sequences (100000161 bp) in 4.090 CPU sec, 4.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306143, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 219, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (208.29, 98.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (284.50, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 189, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 309)
[M::mem_pestat] mean and std.dev: (156.90, 31.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 365)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725574 reads in 98.303 CPU sec, 3.204 real sec
[M::process] read 725548 sequences (100000279 bp) in 3.592 CPU sec, 4.117 real sec...
sorting block 27, #reads = 1102852 mem = 536880936 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305807, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 195, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 314)
[M::mem_pestat] mean and std.dev: (193.64, 51.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 398)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (283.96, 144.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 261, 915)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375)
[M::mem_pestat] mean and std.dev: (357.29, 403.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3105)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725548 reads in 101.257 CPU sec, 3.301 real sec
[M::process] read 725306 sequences (100000093 bp) in 4.222 CPU sec, 4.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306054, 13, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (207.78, 59.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (285.48, 145.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 663, 6383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18895)
[M::mem_pestat] mean and std.dev: (2643.69, 2964.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25151)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 225, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (234.90, 91.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 599)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 28, #reads = 1102660 mem = 536880009 peak = 858259456
[M::mem_process_seqs] Processed 725306 reads in 101.142 CPU sec, 3.312 real sec
[M::process] read 724798 sequences (100000212 bp) in 4.314 CPU sec, 4.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 305637, 3, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 262, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (285.08, 143.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 240, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (198.06, 88.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724798 reads in 99.576 CPU sec, 3.235 real sec
[M::process] read 725458 sequences (100000173 bp) in 2.983 CPU sec, 3.297 real sec...
sorting block 29, #reads = 1102606 mem = 536879131 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306024, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 195, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (211.80, 80.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (284.21, 144.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 159, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (191.53, 81.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725458 reads in 101.070 CPU sec, 3.274 real sec
[M::process] read 725184 sequences (100000095 bp) in 4.556 CPU sec, 5.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306038, 0, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 197, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 412)
[M::mem_pestat] mean and std.dev: (216.08, 58.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (287.23, 146.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (195.58, 60.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725184 reads in 101.054 CPU sec, 3.280 real sec
sorting block 30, #reads = 1102421 mem = 536880928 peak = 858259456
[M::process] read 723986 sequences (100000083 bp) in 4.018 CPU sec, 4.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305308, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 305, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (290.17, 110.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (292.01, 148.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 229, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 330)
[M::mem_pestat] mean and std.dev: (204.67, 33.11)
[M::mem_pestat] low and high boundaries for proper pairs: (72, 372)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723986 reads in 103.974 CPU sec, 3.393 real sec
[M::process] read 723944 sequences (100000225 bp) in 3.388 CPU sec, 3.753 real sec...
sorting block 31, #reads = 1102260 mem = 536879740 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305054, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 230, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (212.55, 78.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 546)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (291.53, 148.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 278, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (246.10, 92.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723944 reads in 95.135 CPU sec, 3.103 real sec
[M::process] read 723600 sequences (100000143 bp) in 3.578 CPU sec, 4.017 real sec...
sorting block 32, #reads = 1101496 mem = 536876981 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304779, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 165, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (200.12, 87.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (292.89, 147.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (107, 206, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (195.58, 90.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723010 sequences (100000078 bp) in 2.601 CPU sec, 2.921 real sec...
[M::mem_process_seqs] Processed 723600 reads in 97.404 CPU sec, 3.168 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304773, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 267, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (241.50, 130.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (294.58, 147.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 248, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790)
[M::mem_pestat] mean and std.dev: (331.85, 244.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2333)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723010 reads in 93.927 CPU sec, 3.054 real sec
[M::process] read 724368 sequences (100000153 bp) in 3.458 CPU sec, 3.865 real sec...
sorting block 33, #reads = 1100589 mem = 536878163 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304394, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (265, 297, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351)
[M::mem_pestat] mean and std.dev: (410.43, 278.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (290.78, 147.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (247.91, 115.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724368 reads in 96.294 CPU sec, 3.135 real sec
[M::process] read 727266 sequences (100000191 bp) in 3.494 CPU sec, 3.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306487, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 237, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (223.33, 93.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (281.15, 144.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 207, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (189.91, 54.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 34, #reads = 1100163 mem = 536877215 peak = 858259456
[M::process] read 726606 sequences (100000277 bp) in 2.705 CPU sec, 3.030 real sec...
[M::mem_process_seqs] Processed 727266 reads in 94.857 CPU sec, 3.084 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306501, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 223, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (220.95, 90.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.93, 144.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 193, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (204.29, 63.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726324 sequences (100000042 bp) in 2.731 CPU sec, 3.032 real sec...
[M::mem_process_seqs] Processed 726606 reads in 92.267 CPU sec, 2.992 real sec
sorting block 35, #reads = 1104268 mem = 536879346 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306003, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 192, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (178.25, 59.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (284.15, 145.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 159, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (194.13, 86.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 559)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726324 reads in 92.597 CPU sec, 3.023 real sec
[M::process] read 725898 sequences (100000225 bp) in 3.038 CPU sec, 3.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305925, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 241, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (218.28, 72.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (283.55, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 244, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (225.77, 95.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725898 reads in 94.042 CPU sec, 3.069 real sec
[M::process] read 725346 sequences (100000031 bp) in 2.549 CPU sec, 3.074 real sec...
sorting block 36, #reads = 1103631 mem = 536880047 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305629, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 179, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (203.75, 83.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.52, 146.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 231, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (222.95, 94.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725606 sequences (100000128 bp) in 2.600 CPU sec, 2.896 real sec...
[M::mem_process_seqs] Processed 725346 reads in 93.307 CPU sec, 3.052 real sec
sorting block 37, #reads = 1102745 mem = 536878361 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305964, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 161, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470)
[M::mem_pestat] mean and std.dev: (187.18, 78.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 579)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (287.28, 147.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 234, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 494)
[M::mem_pestat] mean and std.dev: (229.40, 74.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725606 reads in 86.286 CPU sec, 2.819 real sec
[M::process] read 724770 sequences (100000092 bp) in 3.217 CPU sec, 3.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305545, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 249, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (292.41, 187.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (287.59, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 237, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (211.88, 68.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 38, #reads = 1102404 mem = 536878505 peak = 858259456
[M::mem_process_seqs] Processed 724770 reads in 83.745 CPU sec, 2.732 real sec
[M::process] read 724484 sequences (100000124 bp) in 2.765 CPU sec, 3.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305912, 1, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 210, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (227.86, 86.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (288.20, 145.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 175, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (199.42, 81.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724484 reads in 85.770 CPU sec, 2.805 real sec
[M::process] read 723882 sequences (100000011 bp) in 2.955 CPU sec, 3.332 real sec...
sorting block 39, #reads = 1101843 mem = 536879926 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305165, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 169, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (184.95, 77.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (289.89, 146.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 179, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (185.15, 51.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723882 reads in 92.233 CPU sec, 2.994 real sec
[M::process] read 724214 sequences (100000105 bp) in 3.549 CPU sec, 3.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305270, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 186, 943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2539)
[M::mem_pestat] mean and std.dev: (490.24, 725.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 268, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (288.82, 144.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 290, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (277.46, 130.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724214 reads in 89.675 CPU sec, 2.932 real sec
sorting block 40, #reads = 1101812 mem = 536880053 peak = 858259456
[M::process] read 723650 sequences (100000171 bp) in 3.143 CPU sec, 3.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 304893, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 172, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354)
[M::mem_pestat] mean and std.dev: (182.21, 63.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 435)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (291.23, 146.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 237, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 442)
[M::mem_pestat] mean and std.dev: (215.32, 71.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 529)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723650 reads in 90.366 CPU sec, 2.934 real sec
[M::process] read 723466 sequences (100000042 bp) in 3.302 CPU sec, 3.692 real sec...
sorting block 41, #reads = 1101234 mem = 536879088 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304302, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 275, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (257.78, 116.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (292.09, 147.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 278, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (212.91, 89.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723466 reads in 91.114 CPU sec, 2.964 real sec
[M::process] read 723206 sequences (100000221 bp) in 3.008 CPU sec, 3.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304638, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 224, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (201.85, 64.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 647)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 272, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (294.50, 148.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425)
[M::mem_pestat] mean and std.dev: (200.83, 49.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723206 reads in 91.980 CPU sec, 2.995 real sec
sorting block 42, #reads = 1100485 mem = 536877728 peak = 858259456
[M::process] read 722910 sequences (100000085 bp) in 3.618 CPU sec, 4.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 304740, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 176, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480)
[M::mem_pestat] mean and std.dev: (184.35, 64.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 274, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (295.25, 148.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 223, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (241.53, 123.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722910 reads in 91.235 CPU sec, 2.969 real sec
[M::process] read 722936 sequences (100000005 bp) in 3.111 CPU sec, 3.621 real sec...
sorting block 43, #reads = 1100465 mem = 536879636 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 304421, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 184, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414)
[M::mem_pestat] mean and std.dev: (179.59, 65.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (294.03, 147.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 229, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (246.61, 112.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722936 reads in 92.943 CPU sec, 3.026 real sec
[M::process] read 722874 sequences (100000185 bp) in 2.863 CPU sec, 3.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304249, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 275, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (250.68, 100.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 275, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (295.77, 148.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 203, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (198.23, 69.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722874 reads in 86.731 CPU sec, 2.832 real sec
[M::process] read 722480 sequences (100000076 bp) in 3.007 CPU sec, 3.366 real sec...
sorting block 44, #reads = 1100489 mem = 536879903 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 304354, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 154, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (176.08, 58.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 277, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (298.07, 150.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 244, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525)
[M::mem_pestat] mean and std.dev: (236.69, 73.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 722480 reads in 83.912 CPU sec, 2.735 real sec
[M::process] read 726588 sequences (100000192 bp) in 2.664 CPU sec, 2.945 real sec...
sorting block 45, #reads = 1100123 mem = 536879662 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305893, 7, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 147, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348)
[M::mem_pestat] mean and std.dev: (156.38, 54.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 422)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (285.45, 147.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 352, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (313.50, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726588 reads in 88.136 CPU sec, 2.899 real sec
[M::process] read 728174 sequences (100000058 bp) in 3.296 CPU sec, 3.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 307124, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 179, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (222.89, 106.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (278.61, 144.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 206, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 391)
[M::mem_pestat] mean and std.dev: (193.06, 44.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728174 reads in 91.332 CPU sec, 2.965 real sec
[M::process] read 727250 sequences (100000117 bp) in 3.143 CPU sec, 3.493 real sec...
sorting block 46, #reads = 1101886 mem = 536878489 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306649, 2, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 148, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 239)
[M::mem_pestat] mean and std.dev: (147.28, 25.14)
[M::mem_pestat] low and high boundaries for proper pairs: (29, 274)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.30, 144.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 160, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (157.74, 51.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 473)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727250 reads in 84.104 CPU sec, 2.732 real sec
[M::process] read 726888 sequences (100000165 bp) in 2.790 CPU sec, 3.115 real sec...
sorting block 47, #reads = 1103948 mem = 536879967 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305904, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 202, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (188.37, 63.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.12, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 185, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (183.19, 69.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726888 reads in 88.340 CPU sec, 2.865 real sec
[M::process] read 726742 sequences (100000244 bp) in 3.188 CPU sec, 3.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306169, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 269, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (250.50, 89.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.11, 143.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 205, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (200.93, 74.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 772)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726742 reads in 88.822 CPU sec, 2.883 real sec
sorting block 48, #reads = 1103333 mem = 536879395 peak = 858259456
[M::process] read 726340 sequences (100000046 bp) in 3.607 CPU sec, 4.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306135, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 210, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (252.62, 113.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.37, 144.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726340 reads in 82.431 CPU sec, 2.677 real sec
[M::process] read 726256 sequences (100000032 bp) in 3.025 CPU sec, 3.546 real sec...
sorting block 49, #reads = 1103128 mem = 536878309 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305923, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 235, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (216.60, 70.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.54, 145.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 222, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (221.92, 85.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726256 reads in 84.173 CPU sec, 2.730 real sec
[M::process] read 726122 sequences (100000101 bp) in 2.770 CPU sec, 3.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306119, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 150, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414)
[M::mem_pestat] mean and std.dev: (171.71, 69.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.85, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 189, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (194.43, 59.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 582)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726122 reads in 90.707 CPU sec, 2.937 real sec
[M::process] read 726490 sequences (100000058 bp) in 3.217 CPU sec, 3.549 real sec...
sorting block 50, #reads = 1103229 mem = 536880461 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306039, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 205, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 407)
[M::mem_pestat] mean and std.dev: (199.62, 56.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.35, 144.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 203, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (197.17, 73.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726490 reads in 89.702 CPU sec, 2.932 real sec
[M::process] read 727442 sequences (100000228 bp) in 3.172 CPU sec, 3.716 real sec...
sorting block 51, #reads = 1103180 mem = 536877758 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306573, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 232, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (259.50, 157.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (278.77, 142.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 246, 3208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9294)
[M::mem_pestat] mean and std.dev: (1347.07, 1839.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12337)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727442 reads in 89.433 CPU sec, 2.899 real sec
[M::process] read 727996 sequences (100000188 bp) in 3.527 CPU sec, 3.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 307026, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 162, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (192.00, 86.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.84, 141.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727996 reads in 88.440 CPU sec, 2.866 real sec
sorting block 52, #reads = 1103833 mem = 536877767 peak = 858259456
[M::process] read 727962 sequences (100000286 bp) in 3.363 CPU sec, 3.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 307153, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 207, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (204.23, 85.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.50, 141.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 181, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (190.86, 91.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727962 reads in 87.051 CPU sec, 2.831 real sec
[M::process] read 728048 sequences (100000122 bp) in 3.175 CPU sec, 3.475 real sec...
sorting block 53, #reads = 1103973 mem = 536877677 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 307115, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 193, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (197.69, 86.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (277.09, 142.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728048 reads in 87.468 CPU sec, 2.835 real sec
[M::process] read 726450 sequences (100000108 bp) in 3.481 CPU sec, 3.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306095, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 297, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (239.76, 83.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.53, 145.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 197, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435)
[M::mem_pestat] mean and std.dev: (200.67, 66.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 530)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726450 reads in 86.362 CPU sec, 2.795 real sec
sorting block 54, #reads = 1104543 mem = 536879347 peak = 858259456
[M::process] read 725086 sequences (100000123 bp) in 3.127 CPU sec, 3.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305718, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 202, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (207.76, 64.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (288.14, 147.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 168, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (187.53, 77.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 498)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725086 reads in 85.665 CPU sec, 2.773 real sec
[M::process] read 724310 sequences (100000208 bp) in 2.797 CPU sec, 3.119 real sec...
sorting block 55, #reads = 1102689 mem = 536877461 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305172, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 199, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 335)
[M::mem_pestat] mean and std.dev: (186.46, 56.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 412)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (293.23, 150.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 168, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480)
[M::mem_pestat] mean and std.dev: (183.46, 60.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724310 reads in 86.708 CPU sec, 2.809 real sec
[M::process] read 724000 sequences (100000162 bp) in 3.304 CPU sec, 3.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 304544, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 199, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (211.08, 69.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 619)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (293.62, 149.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 174, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (192.71, 72.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727138 sequences (100000010 bp) in 2.501 CPU sec, 2.824 real sec...
[M::mem_process_seqs] Processed 724000 reads in 90.125 CPU sec, 2.931 real sec
sorting block 56, #reads = 1101787 mem = 536878082 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 306503, 2, 27)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (283.62, 147.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 195, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (209.48, 71.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727138 reads in 85.810 CPU sec, 2.785 real sec
[M::process] read 728576 sequences (100000176 bp) in 3.215 CPU sec, 3.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307190, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 181, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 333)
[M::mem_pestat] mean and std.dev: (181.62, 49.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (277.01, 143.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 199, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (186.00, 92.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 715)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728576 reads in 86.861 CPU sec, 2.856 real sec
sorting block 57, #reads = 1101283 mem = 536879512 peak = 858259456
[M::process] read 727760 sequences (100000235 bp) in 3.208 CPU sec, 3.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307067, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (208.58, 111.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 728)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (279.98, 144.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 168, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (170.11, 73.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727760 reads in 90.499 CPU sec, 3.010 real sec
[M::process] read 727132 sequences (100000114 bp) in 3.266 CPU sec, 3.878 real sec...
sorting block 58, #reads = 1105322 mem = 536880079 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306185, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 158, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (157.00, 51.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.93, 144.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (219.25, 88.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727132 reads in 93.613 CPU sec, 3.071 real sec
[M::process] read 726824 sequences (100000187 bp) in 3.476 CPU sec, 3.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306170, 0, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 229, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (215.56, 67.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.61, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 182, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (184.89, 80.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 59, #reads = 1104144 mem = 536881322 peak = 858259456
[M::mem_process_seqs] Processed 726824 reads in 93.763 CPU sec, 3.075 real sec
[M::process] read 726522 sequences (100000053 bp) in 3.010 CPU sec, 3.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305692, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 216, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (221.15, 96.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.66, 144.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 195, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (184.12, 62.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726522 reads in 96.959 CPU sec, 3.161 real sec
[M::process] read 726422 sequences (100000279 bp) in 3.056 CPU sec, 3.418 real sec...
sorting block 60, #reads = 1103021 mem = 536879444 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306236, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 254, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (237.44, 74.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.82, 144.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 190, 808)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192)
[M::mem_pestat] mean and std.dev: (279.75, 277.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726422 reads in 93.294 CPU sec, 3.079 real sec
[M::process] read 726146 sequences (100000267 bp) in 3.380 CPU sec, 3.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306353, 7, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 222, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (285.47, 121.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.78, 144.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 227, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (213.92, 110.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726146 reads in 94.845 CPU sec, 3.103 real sec
sorting block 61, #reads = 1103061 mem = 536879148 peak = 858259456
[M::process] read 725994 sequences (100000010 bp) in 3.322 CPU sec, 3.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306285, 8, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 196, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (199.27, 61.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 577)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.16, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 192, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (204.31, 81.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725994 reads in 95.425 CPU sec, 3.112 real sec
[M::process] read 726594 sequences (100000062 bp) in 3.411 CPU sec, 3.795 real sec...
sorting block 62, #reads = 1103345 mem = 536879965 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306252, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 165, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (183.74, 86.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.82, 143.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 177, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (187.92, 66.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 478)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726594 reads in 97.662 CPU sec, 3.209 real sec
[M::process] read 727218 sequences (100000231 bp) in 3.239 CPU sec, 3.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306484, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 297, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (238.43, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 255, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (279.52, 143.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 247, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (214.80, 66.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 583)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727218 reads in 99.964 CPU sec, 3.286 real sec
sorting block 63, #reads = 1102984 mem = 536879999 peak = 858259456
[M::process] read 726862 sequences (100000068 bp) in 4.166 CPU sec, 4.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306327, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 208, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (208.00, 133.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (280.90, 143.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 205, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (201.14, 91.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726862 reads in 95.721 CPU sec, 3.116 real sec
[M::process] read 726848 sequences (100000148 bp) in 3.481 CPU sec, 3.947 real sec...
sorting block 64, #reads = 1103397 mem = 536878468 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306560, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 207, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 394)
[M::mem_pestat] mean and std.dev: (191.14, 61.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (282.24, 145.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 303, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (248.21, 90.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726848 reads in 93.537 CPU sec, 3.071 real sec
[M::process] read 725242 sequences (100000221 bp) in 3.182 CPU sec, 3.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 305480, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 205, 1165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3191)
[M::mem_pestat] mean and std.dev: (351.91, 419.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4204)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (287.81, 147.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 185, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (213.27, 74.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725242 reads in 97.397 CPU sec, 3.199 real sec
sorting block 65, #reads = 1103887 mem = 536880001 peak = 858259456
[M::process] read 724898 sequences (100000087 bp) in 3.498 CPU sec, 4.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305356, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 238, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (226.94, 86.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (291.10, 149.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 144, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (173.05, 61.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724898 reads in 94.684 CPU sec, 3.086 real sec
[M::process] read 724324 sequences (100000003 bp) in 3.514 CPU sec, 3.874 real sec...
sorting block 66, #reads = 1102401 mem = 536877449 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305484, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 188, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (185.82, 56.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (293.31, 151.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 234, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (225.62, 78.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724324 reads in 96.667 CPU sec, 3.161 real sec
[M::process] read 724386 sequences (100000077 bp) in 3.682 CPU sec, 4.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305350, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 156, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (150.54, 47.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (295.56, 152.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 278, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (237.69, 100.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726990 sequences (100000065 bp) in 2.867 CPU sec, 3.173 real sec...
[M::mem_process_seqs] Processed 724386 reads in 98.924 CPU sec, 3.228 real sec
sorting block 67, #reads = 1100929 mem = 536878757 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306069, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (105, 197, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (267.70, 211.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 259, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (285.81, 149.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 193, 662)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1692)
[M::mem_pestat] mean and std.dev: (263.53, 183.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2207)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726990 reads in 99.984 CPU sec, 3.282 real sec
[M::process] read 728590 sequences (100000201 bp) in 3.203 CPU sec, 3.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306964, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 201, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (176.25, 50.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (276.51, 142.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 174, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364)
[M::mem_pestat] mean and std.dev: (168.81, 50.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 438)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728590 reads in 97.091 CPU sec, 3.224 real sec
sorting block 68, #reads = 1100932 mem = 536878140 peak = 858259456
[M::process] read 727926 sequences (100000204 bp) in 4.071 CPU sec, 4.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306937, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 154, 1280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3552)
[M::mem_pestat] mean and std.dev: (460.32, 644.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4688)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.80, 143.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 167, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (186.19, 67.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727926 reads in 98.431 CPU sec, 3.263 real sec
[M::process] read 727488 sequences (100000250 bp) in 3.326 CPU sec, 3.839 real sec...
sorting block 69, #reads = 1104260 mem = 536878159 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306999, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 201, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (191.46, 54.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (281.64, 145.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 192, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426)
[M::mem_pestat] mean and std.dev: (276.69, 179.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727488 reads in 97.822 CPU sec, 3.231 real sec
[M::process] read 726936 sequences (100000041 bp) in 3.661 CPU sec, 4.042 real sec...
sorting block 70, #reads = 1104345 mem = 536877514 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306602, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 208, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (75, 375)
[M::mem_pestat] mean and std.dev: (201.72, 52.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 435)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.79, 145.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 219, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470)
[M::mem_pestat] mean and std.dev: (232.23, 98.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 628)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726936 reads in 98.587 CPU sec, 3.255 real sec
[M::process] read 726288 sequences (100000028 bp) in 3.664 CPU sec, 4.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306187, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 241, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (212.43, 72.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (283.78, 145.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 155, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (178.62, 66.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 566)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726288 reads in 98.038 CPU sec, 3.228 real sec
sorting block 71, #reads = 1104076 mem = 536879390 peak = 858259456
[M::process] read 726088 sequences (100000122 bp) in 3.689 CPU sec, 4.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306390, 10, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 169, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 278)
[M::mem_pestat] mean and std.dev: (142.82, 33.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (283.64, 144.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 28, 83)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207)
[M::mem_pestat] mean and std.dev: (43.11, 36.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 269)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 135, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (166.17, 78.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726088 reads in 98.737 CPU sec, 3.236 real sec
[M::process] read 725910 sequences (100000218 bp) in 3.222 CPU sec, 3.658 real sec...
sorting block 72, #reads = 1103123 mem = 536875708 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306072, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 236, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (317.89, 204.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.40, 144.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 195, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (209.62, 82.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725936 sequences (100000148 bp) in 3.010 CPU sec, 3.310 real sec...
[M::mem_process_seqs] Processed 725910 reads in 101.118 CPU sec, 3.327 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306418, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 227, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500)
[M::mem_pestat] mean and std.dev: (201.24, 67.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (285.66, 146.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 141, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (179.39, 91.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725936 reads in 104.643 CPU sec, 3.477 real sec
[M::process] read 725428 sequences (100000109 bp) in 3.565 CPU sec, 3.899 real sec...
sorting block 73, #reads = 1102926 mem = 536877224 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 305786, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (285.34, 144.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 188, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (183.58, 64.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725428 reads in 106.617 CPU sec, 3.554 real sec
[M::process] read 725366 sequences (100000267 bp) in 3.783 CPU sec, 4.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305548, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 196, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (208.16, 73.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.74, 145.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 208, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420)
[M::mem_pestat] mean and std.dev: (187.38, 71.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725366 reads in 101.922 CPU sec, 3.376 real sec
sorting block 74, #reads = 1103106 mem = 536879285 peak = 858259456
[M::process] read 725864 sequences (100000050 bp) in 3.564 CPU sec, 4.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305665, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 172, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 253)
[M::mem_pestat] mean and std.dev: (160.91, 41.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (283.94, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 242, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (273.62, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725864 reads in 101.234 CPU sec, 3.383 real sec
[M::process] read 725618 sequences (100000133 bp) in 3.596 CPU sec, 4.182 real sec...
sorting block 75, #reads = 1101976 mem = 536877864 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 305665, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 215, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (214.76, 90.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (285.05, 146.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 725618 reads in 102.800 CPU sec, 3.420 real sec
[M::process] read 724802 sequences (100000189 bp) in 3.537 CPU sec, 3.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305588, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 205, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (206.25, 77.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (288.43, 146.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 238, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (199.12, 57.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 533)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724802 reads in 101.051 CPU sec, 3.376 real sec
[M::process] read 724504 sequences (100000256 bp) in 3.414 CPU sec, 3.734 real sec...
sorting block 76, #reads = 1102319 mem = 536878494 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305049, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 190, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (222.21, 113.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (290.91, 148.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (279.50, 101.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724504 reads in 103.547 CPU sec, 3.478 real sec
[M::process] read 724762 sequences (100000040 bp) in 3.142 CPU sec, 3.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 305391, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 215, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 381)
[M::mem_pestat] mean and std.dev: (224.17, 76.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (292.46, 150.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (103, 135, 171)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307)
[M::mem_pestat] mean and std.dev: (133.79, 52.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 375)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724762 reads in 104.874 CPU sec, 3.500 real sec
sorting block 77, #reads = 1102165 mem = 536878053 peak = 858259456
[M::process] read 724520 sequences (100000194 bp) in 3.574 CPU sec, 3.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304977, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 244, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (239.11, 90.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 269, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (295.07, 153.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 178, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 359)
[M::mem_pestat] mean and std.dev: (173.93, 40.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 430)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724520 reads in 106.200 CPU sec, 3.545 real sec
[M::process] read 725598 sequences (100000007 bp) in 3.735 CPU sec, 4.121 real sec...
sorting block 78, #reads = 1101536 mem = 536878105 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305280, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 192, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (180.71, 50.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 474)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (291.54, 153.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 235, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (244.64, 101.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725598 reads in 99.295 CPU sec, 3.331 real sec
[M::process] read 728210 sequences (100000019 bp) in 3.620 CPU sec, 3.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307373, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 175, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357)
[M::mem_pestat] mean and std.dev: (168.67, 30.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 429)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (278.06, 143.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 207, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (209.18, 105.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728210 reads in 98.455 CPU sec, 3.284 real sec
sorting block 79, #reads = 1100774 mem = 536876906 peak = 858259456
[M::process] read 727416 sequences (100000238 bp) in 3.643 CPU sec, 4.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306372, 11, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 182, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (191.85, 62.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (278.88, 143.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 75, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (124.10, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 161, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 356)
[M::mem_pestat] mean and std.dev: (178.52, 50.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727416 reads in 102.514 CPU sec, 3.445 real sec
[M::process] read 726832 sequences (100000087 bp) in 3.878 CPU sec, 4.265 real sec...
sorting block 80, #reads = 1102428 mem = 536876387 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306633, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 189, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 392)
[M::mem_pestat] mean and std.dev: (194.95, 58.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (280.54, 143.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 145, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (145.17, 53.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726832 reads in 102.167 CPU sec, 3.438 real sec
[M::process] read 726566 sequences (100000108 bp) in 3.586 CPU sec, 4.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306592, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 237, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482)
[M::mem_pestat] mean and std.dev: (221.72, 67.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.76, 145.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 176, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (241.60, 150.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726566 reads in 100.347 CPU sec, 3.366 real sec
sorting block 81, #reads = 1103987 mem = 536881162 peak = 858259456
[M::process] read 725964 sequences (100000249 bp) in 3.543 CPU sec, 3.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305923, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 230, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (237.00, 90.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (285.18, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 226, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (208.90, 49.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 547)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725964 reads in 102.234 CPU sec, 3.436 real sec
[M::process] read 725802 sequences (100000152 bp) in 3.302 CPU sec, 3.598 real sec...
sorting block 82, #reads = 1103490 mem = 536878698 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305559, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 244, 1775)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5071)
[M::mem_pestat] mean and std.dev: (564.46, 667.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (285.86, 145.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 211, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (204.75, 70.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725802 reads in 101.427 CPU sec, 3.403 real sec
[M::process] read 725770 sequences (100000234 bp) in 3.616 CPU sec, 3.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306264, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 209, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420)
[M::mem_pestat] mean and std.dev: (204.00, 79.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (285.75, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 222, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (229.71, 77.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725770 reads in 103.396 CPU sec, 3.465 real sec
sorting block 83, #reads = 1102844 mem = 536878316 peak = 858259456
[M::process] read 725568 sequences (100000182 bp) in 3.982 CPU sec, 4.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305983, 6, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 267, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (257.11, 111.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 263, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (287.23, 146.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 189, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (202.48, 76.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 550)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725568 reads in 101.677 CPU sec, 3.413 real sec
[M::process] read 724914 sequences (100000250 bp) in 3.294 CPU sec, 3.801 real sec...
sorting block 84, #reads = 1102270 mem = 536878146 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 305490, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 192, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (170.22, 33.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (287.34, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 212, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (222.44, 80.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724914 reads in 104.389 CPU sec, 3.511 real sec
[M::process] read 724864 sequences (100000259 bp) in 3.773 CPU sec, 4.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305496, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 161, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (189.45, 59.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (289.35, 147.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 247, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 452)
[M::mem_pestat] mean and std.dev: (228.85, 46.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724864 reads in 103.538 CPU sec, 3.466 real sec
[M::process] read 724776 sequences (100000249 bp) in 3.601 CPU sec, 3.911 real sec...
sorting block 85, #reads = 1102724 mem = 536878960 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305275, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 215, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (220.82, 102.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (288.20, 146.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (107, 185, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (157.00, 54.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 535)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724776 reads in 98.140 CPU sec, 3.292 real sec
[M::process] read 724716 sequences (100000002 bp) in 3.752 CPU sec, 4.118 real sec...
sorting block 86, #reads = 1102010 mem = 536878195 peak = 858259456
[M::process] read 724266 sequences (100000032 bp) in 2.336 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305088, 9, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 169, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396)
[M::mem_pestat] mean and std.dev: (160.60, 74.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (289.34, 147.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 227, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (207.60, 89.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305397, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 197, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (205.62, 69.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 610)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (290.77, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 275, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (238.09, 86.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724716 reads in 184.840 CPU sec, 5.977 real sec
[M::mem_process_seqs] Processed 724266 reads in 105.639 CPU sec, 3.393 real sec
sorting block 87, #reads = 1102087 mem = 536878274 peak = 858259456
[M::process] read 724136 sequences (100000000 bp) in 3.692 CPU sec, 4.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304770, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 186, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (197.76, 74.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (292.99, 149.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 235, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449)
[M::mem_pestat] mean and std.dev: (190.67, 54.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 546)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724136 reads in 97.165 CPU sec, 3.253 real sec
[M::process] read 724194 sequences (100000255 bp) in 3.467 CPU sec, 3.864 real sec...
sorting block 88, #reads = 1101559 mem = 536880910 peak = 858259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305400, 1, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 176, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (184.38, 67.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 270, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (293.81, 150.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 261, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (242.23, 122.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724194 reads in 95.105 CPU sec, 3.177 real sec
[M::process] read 723976 sequences (100000044 bp) in 3.322 CPU sec, 3.836 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304710, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 151, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (179.65, 70.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 271, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (295.38, 151.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 325, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (296.86, 107.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723976 reads in 95.296 CPU sec, 3.183 real sec
[M::process] read 724016 sequences (100000142 bp) in 3.317 CPU sec, 3.697 real sec...
sorting block 89, #reads = 1101313 mem = 536877999 peak = 858259456
[M::process] read 70500 sequences (9729027 bp) in 0.556 CPU sec, 0.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 304799, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 152, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (192.82, 74.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 270, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (296.35, 153.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 157, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (170.90, 51.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 29816, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 271, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (296.69, 153.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1101413 mem = 536879339 peak = 858259456
[M::mem_process_seqs] Processed 724016 reads in 105.514 CPU sec, 3.512 real sec
[M::mem_process_seqs] Processed 70500 reads in 20.541 CPU sec, 0.686 real sec
sorting block 91, #reads = 1100808 mem = 536878979 peak = 858259456
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 100951094
overall: 69429047296 mem 15492.219 user 1156.569 sys 648.978 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.SH032_HG514.L007\tPU:L007\tSM:SH032_HG514\tLB:SH032_HG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.SH032_HG514_R1.fastq.gz 0004.SH032_HG514_R2.fastq.gz
[main] Real time: 649.017 sec; CPU: 16648.792 sec
sorting block 92, #reads = 94088 mem = 45913204 peak = 858259456
uniq header count 1
merging 93 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 4454306147
output reads: 101538979
bam_mem_sort: 93 calls 67.472 user 1.673 sys 69.236 real
bam_write: 93 calls 33.836 user 2.240 sys 36.622 real
execute: 1 calls 29.997 user 2.125 sys 682.141 real
merge_files: 1 calls 29.961 user 2.101 sys 33.069 real
parse_chunk: 1474 calls 64.524 user 17.823 sys 83.788 real
read_chunk: 3990 calls 52.137 user 33.200 sys 528.227 real
sort_block: 1 calls 41.061 user 32.174 sys 512.368 real
write_chunk: 2514 calls 29.536 user 1.706 sys 31.917 real
overall: 2312286208 mem 1161.725 user 78.259 sys 682.165 real
ls: cannot access '*.cram': No such file or directory