File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/aeee274d137e6f6ce4350bb4b02cb0/.command.err
Size
208.5 KB
Attempt
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1_25PercentHG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 3217.826 CPU sec, 135.653 real sec
[M::process] read 732436 sequences (100000013 bp) in 1.067 CPU sec, 1.076 real sec...
[M::process] read 731154 sequences (100000019 bp) in 1.709 CPU sec, 1.748 real sec...
[M::process] read 731044 sequences (100000154 bp) in 1.841 CPU sec, 1.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 310078, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (261.34, 132.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731084 sequences (100000063 bp) in 1.430 CPU sec, 1.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309621, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.10, 135.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309022, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.07, 135.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732436 reads in 310.242 CPU sec, 10.265 real sec
[M::mem_process_seqs] Processed 731044 reads in 198.927 CPU sec, 6.764 real sec
[M::mem_process_seqs] Processed 731154 reads in 209.872 CPU sec, 7.190 real sec
sorting block 0, #reads = 1097200 mem = 536878714 peak = 587268096
[M::process] read 730480 sequences (100000172 bp) in 2.486 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308901, 0, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (185.30, 99.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.20, 134.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 184, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (191.00, 94.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 1, #reads = 1095965 mem = 536877961 peak = 766574592
[M::process] read 730422 sequences (100000092 bp) in 1.758 CPU sec, 1.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308370, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (266.81, 135.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 174, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (170.50, 55.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 487)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731084 reads in 192.764 CPU sec, 6.243 real sec
[M::process] read 730248 sequences (100000232 bp) in 1.597 CPU sec, 1.670 real sec...
[M::mem_process_seqs] Processed 730480 reads in 118.086 CPU sec, 3.813 real sec
sorting block 2, #reads = 1095853 mem = 536882681 peak = 766574592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308871, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 163, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (179.00, 66.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.44, 135.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730590 sequences (100000077 bp) in 2.560 CPU sec, 2.718 real sec...
[M::process] read 731424 sequences (100000145 bp) in 1.531 CPU sec, 1.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308818, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 154, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (180.45, 66.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 768)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (267.81, 135.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 180, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 319)
[M::mem_pestat] mean and std.dev: (186.00, 40.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 373)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730422 reads in 188.458 CPU sec, 6.044 real sec
[M::mem_process_seqs] Processed 730248 reads in 111.707 CPU sec, 3.619 real sec
sorting block 3, #reads = 1095609 mem = 536881204 peak = 766574592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308737, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.38, 136.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731352 sequences (100000152 bp) in 1.954 CPU sec, 2.142 real sec...
[M::process] read 731188 sequences (100000017 bp) in 1.592 CPU sec, 1.598 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309488, 9, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.90, 134.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 329, 603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429)
[M::mem_pestat] mean and std.dev: (339.70, 187.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1842)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730590 reads in 186.438 CPU sec, 5.991 real sec
[M::mem_process_seqs] Processed 731424 reads in 111.843 CPU sec, 3.607 real sec
sorting block 4, #reads = 1095815 mem = 536877903 peak = 766574592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308577, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.98, 134.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 208, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (193.70, 67.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 597)
[M::mem_pestat] skip orientation RR
sorting block 5, #reads = 1096266 mem = 536875252 peak = 766574592
[M::process] read 731258 sequences (100000156 bp) in 2.475 CPU sec, 2.687 real sec...
[M::process] read 731002 sequences (100000269 bp) in 1.220 CPU sec, 1.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309316, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (263.73, 134.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731352 reads in 183.784 CPU sec, 5.910 real sec
[M::mem_process_seqs] Processed 731188 reads in 105.897 CPU sec, 3.416 real sec
sorting block 6, #reads = 1096265 mem = 536881059 peak = 766574592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309131, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.76, 134.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731220 sequences (100000005 bp) in 2.541 CPU sec, 2.791 real sec...
[M::process] read 731330 sequences (100000024 bp) in 1.650 CPU sec, 1.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 308882, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 173, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (102, 242)
[M::mem_pestat] mean and std.dev: (165.59, 24.35)
[M::mem_pestat] low and high boundaries for proper pairs: (68, 270)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (264.19, 134.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 203, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (224.30, 133.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731258 reads in 186.515 CPU sec, 5.981 real sec
[M::mem_process_seqs] Processed 731002 reads in 110.723 CPU sec, 3.554 real sec
sorting block 7, #reads = 1096771 mem = 536876860 peak = 766574592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309342, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (96, 242, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (224.90, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.92, 134.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 182, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (180.80, 52.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 545)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 731120 sequences (100000268 bp) in 2.061 CPU sec, 2.204 real sec...
[M::process] read 731256 sequences (100000218 bp) in 1.408 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309065, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 139, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (167.30, 82.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (263.25, 133.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (104, 151, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (169.09, 75.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731220 reads in 188.738 CPU sec, 6.100 real sec
[M::mem_process_seqs] Processed 731330 reads in 107.961 CPU sec, 3.496 real sec
sorting block 8, #reads = 1096260 mem = 536877039 peak = 766574592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309081, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.84, 134.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731106 sequences (100000240 bp) in 2.448 CPU sec, 2.680 real sec...
sorting block 9, #reads = 1096500 mem = 536879859 peak = 777150464
[M::process] read 730970 sequences (100000079 bp) in 1.142 CPU sec, 1.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309491, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 240, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (264.63, 134.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731120 reads in 180.491 CPU sec, 5.799 real sec
[M::mem_process_seqs] Processed 731256 reads in 106.866 CPU sec, 3.476 real sec
sorting block 10, #reads = 1096216 mem = 536879359 peak = 777150464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 309156, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 174, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (161.40, 58.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.18, 134.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 206, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (206.00, 52.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729910 sequences (100000269 bp) in 2.446 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308744, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 264, 4348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12772)
[M::mem_pestat] mean and std.dev: (1229.58, 1801.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (265.80, 135.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 167, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (260.73, 162.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729778 sequences (100000197 bp) in 1.815 CPU sec, 1.823 real sec...
[M::mem_process_seqs] Processed 731106 reads in 183.237 CPU sec, 5.916 real sec
[M::mem_process_seqs] Processed 730970 reads in 106.403 CPU sec, 3.447 real sec
sorting block 11, #reads = 1096481 mem = 536878934 peak = 777150464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308427, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 173, 193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 309)
[M::mem_pestat] mean and std.dev: (158.00, 39.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 244, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (267.23, 135.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729498 sequences (100000134 bp) in 2.190 CPU sec, 2.308 real sec...
[M::process] read 729026 sequences (100000077 bp) in 1.549 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308242, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (269.74, 137.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 207, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (93, 363)
[M::mem_pestat] mean and std.dev: (224.45, 60.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729910 reads in 173.816 CPU sec, 5.571 real sec
[M::mem_process_seqs] Processed 729778 reads in 101.061 CPU sec, 3.255 real sec
sorting block 12, #reads = 1095894 mem = 536878762 peak = 777150464
sorting block 13, #reads = 1094887 mem = 536878645 peak = 839507968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308126, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (270.69, 137.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729006 sequences (100000202 bp) in 2.494 CPU sec, 2.679 real sec...
[M::process] read 726412 sequences (100000067 bp) in 1.843 CPU sec, 1.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308069, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 182, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 331)
[M::mem_pestat] mean and std.dev: (173.27, 50.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (272.00, 138.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729498 reads in 187.101 CPU sec, 5.973 real sec
[M::mem_process_seqs] Processed 729026 reads in 105.654 CPU sec, 3.368 real sec
sorting block 14, #reads = 1094705 mem = 536877920 peak = 839507968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307904, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 167, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (150.38, 40.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 567)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (270.43, 136.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 157, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 240)
[M::mem_pestat] mean and std.dev: (149.42, 37.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 730800 sequences (100000115 bp) in 2.612 CPU sec, 2.891 real sec...
[M::process] read 731734 sequences (100000082 bp) in 1.672 CPU sec, 1.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306390, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 169, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (168.90, 58.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (278.90, 140.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729006 reads in 197.842 CPU sec, 6.344 real sec
[M::mem_process_seqs] Processed 726412 reads in 115.395 CPU sec, 3.706 real sec
sorting block 15, #reads = 1094048 mem = 536878827 peak = 839507968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309128, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (199.18, 68.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 243, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.22, 135.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731020 sequences (100000072 bp) in 2.555 CPU sec, 2.838 real sec...
[M::process] read 730938 sequences (100000059 bp) in 1.255 CPU sec, 1.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309616, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.30, 134.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730800 reads in 197.513 CPU sec, 6.350 real sec
[M::mem_process_seqs] Processed 731734 reads in 110.977 CPU sec, 3.590 real sec
sorting block 16, #reads = 1092361 mem = 536880622 peak = 839507968
sorting block 17, #reads = 1097169 mem = 536879574 peak = 839507968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308927, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (264.96, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731036 sequences (100000115 bp) in 2.131 CPU sec, 2.274 real sec...
[M::process] read 730688 sequences (100000008 bp) in 1.739 CPU sec, 1.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309065, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.38, 135.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731020 reads in 200.911 CPU sec, 6.460 real sec
[M::mem_process_seqs] Processed 730938 reads in 118.062 CPU sec, 3.799 real sec
sorting block 18, #reads = 1096374 mem = 536881636 peak = 839507968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 308859, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 205, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (243.33, 74.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.65, 135.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730628 sequences (100000107 bp) in 2.672 CPU sec, 2.940 real sec...
[M::process] read 730204 sequences (100000217 bp) in 1.387 CPU sec, 1.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308956, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (266.86, 135.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731036 reads in 195.570 CPU sec, 6.257 real sec
[M::mem_process_seqs] Processed 730688 reads in 109.857 CPU sec, 3.519 real sec
sorting block 19, #reads = 1095907 mem = 536878704 peak = 839507968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308898, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.35, 136.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729706 sequences (100000202 bp) in 2.415 CPU sec, 2.584 real sec...
[M::process] read 729976 sequences (100000140 bp) in 1.680 CPU sec, 1.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308588, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 157, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425)
[M::mem_pestat] mean and std.dev: (172.79, 51.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (267.79, 135.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 206, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (192.70, 65.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730628 reads in 200.358 CPU sec, 6.425 real sec
[M::mem_process_seqs] Processed 730204 reads in 114.620 CPU sec, 3.687 real sec
sorting block 20, #reads = 1095830 mem = 536879667 peak = 839507968
sorting block 21, #reads = 1095477 mem = 536879941 peak = 839507968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308467, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.32, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 200, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (205.22, 69.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RR
[M::process] read 729686 sequences (100000145 bp) in 2.536 CPU sec, 2.735 real sec...
[M::process] read 729494 sequences (100000139 bp) in 1.630 CPU sec, 1.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308567, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.33, 137.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729706 reads in 193.068 CPU sec, 6.184 real sec
[M::mem_process_seqs] Processed 729976 reads in 107.426 CPU sec, 3.453 real sec
sorting block 22, #reads = 1095268 mem = 536880733 peak = 839507968
[M::process] read 729648 sequences (100000158 bp) in 1.757 CPU sec, 1.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308680, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.45, 137.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729548 sequences (100000099 bp) in 1.182 CPU sec, 1.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308287, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.66, 137.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308464, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (269.14, 136.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (93, 250, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (188.92, 99.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729686 reads in 259.289 CPU sec, 8.340 real sec
[M::mem_process_seqs] Processed 729648 reads in 184.147 CPU sec, 5.968 real sec
[M::mem_process_seqs] Processed 729494 reads in 192.186 CPU sec, 6.262 real sec
sorting block 23, #reads = 1095423 mem = 536880243 peak = 839507968
[M::process] read 729664 sequences (100000196 bp) in 2.148 CPU sec, 2.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308035, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.26, 137.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1095125 mem = 536877521 peak = 839507968
[M::process] read 729164 sequences (100000188 bp) in 2.073 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308227, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.82, 137.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729548 reads in 185.314 CPU sec, 5.944 real sec
[M::mem_process_seqs] Processed 729664 reads in 105.677 CPU sec, 3.362 real sec
[M::process] read 729428 sequences (100000190 bp) in 1.766 CPU sec, 1.787 real sec...
sorting block 25, #reads = 1094793 mem = 536877259 peak = 839507968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308277, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (269.55, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 181, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (190.23, 68.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RR
[M::process] read 729312 sequences (100000009 bp) in 2.263 CPU sec, 2.426 real sec...
[M::process] read 729118 sequences (100000026 bp) in 1.220 CPU sec, 1.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308284, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 204, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (224.00, 108.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (270.78, 137.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729164 reads in 170.741 CPU sec, 5.457 real sec
[M::mem_process_seqs] Processed 729428 reads in 96.626 CPU sec, 3.100 real sec
sorting block 26, #reads = 1094781 mem = 536878935 peak = 839507968
sorting block 27, #reads = 1094702 mem = 536879719 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308184, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 229, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (236.00, 109.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.50, 137.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729850 sequences (100000203 bp) in 2.286 CPU sec, 2.426 real sec...
[M::process] read 729868 sequences (100000030 bp) in 1.270 CPU sec, 1.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308414, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.75, 137.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729312 reads in 174.480 CPU sec, 5.576 real sec
[M::mem_process_seqs] Processed 729118 reads in 96.020 CPU sec, 3.079 real sec
sorting block 28, #reads = 1095052 mem = 536878220 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308590, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 729072 sequences (100000005 bp) in 1.868 CPU sec, 1.974 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (269.19, 136.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728796 sequences (100000229 bp) in 1.401 CPU sec, 1.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308381, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 167, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437)
[M::mem_pestat] mean and std.dev: (173.33, 55.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.38, 137.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 729850 reads in 179.177 CPU sec, 5.746 real sec
[M::mem_process_seqs] Processed 729868 reads in 101.930 CPU sec, 3.270 real sec
sorting block 29, #reads = 1094989 mem = 536880104 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307944, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (272.58, 139.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 184, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (194.79, 78.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RR
[M::process] read 729592 sequences (100000247 bp) in 2.345 CPU sec, 2.532 real sec...
[M::process] read 730406 sequences (100000146 bp) in 1.580 CPU sec, 1.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307674, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (273.28, 139.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729072 reads in 183.798 CPU sec, 5.880 real sec
[M::mem_process_seqs] Processed 728796 reads in 104.272 CPU sec, 3.335 real sec
sorting block 30, #reads = 1094844 mem = 536879401 peak = 852692992
sorting block 31, #reads = 1094033 mem = 536878106 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308382, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (269.93, 137.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 201, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364)
[M::mem_pestat] mean and std.dev: (161.77, 52.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 440)
[M::mem_pestat] skip orientation RR
[M::process] read 729312 sequences (100000161 bp) in 2.334 CPU sec, 2.460 real sec...
[M::process] read 732926 sequences (100000183 bp) in 1.450 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308640, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (268.10, 137.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729592 reads in 165.024 CPU sec, 5.280 real sec
[M::mem_process_seqs] Processed 730406 reads in 89.893 CPU sec, 2.853 real sec
sorting block 32, #reads = 1094994 mem = 536879043 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308442, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.86, 136.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 157, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (179.83, 57.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] skip orientation RR
[M::process] read 732224 sequences (100000052 bp) in 2.376 CPU sec, 2.584 real sec...
[M::process] read 731362 sequences (100000199 bp) in 1.750 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 310172, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (260.55, 133.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 172, 803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169)
[M::mem_pestat] mean and std.dev: (217.25, 223.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2852)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729312 reads in 169.973 CPU sec, 5.437 real sec
[M::mem_process_seqs] Processed 732926 reads in 97.112 CPU sec, 3.140 real sec
sorting block 33, #reads = 1095029 mem = 536881607 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309496, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (261.49, 132.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731034 sequences (100000269 bp) in 2.183 CPU sec, 2.309 real sec...
[M::process] read 731360 sequences (100000210 bp) in 1.620 CPU sec, 1.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309022, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.34, 134.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 40, 49)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 71)
[M::mem_pestat] mean and std.dev: (38.40, 11.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 83)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 732224 reads in 164.227 CPU sec, 5.246 real sec
[M::mem_process_seqs] Processed 731362 reads in 94.748 CPU sec, 3.016 real sec
sorting block 34, #reads = 1097019 mem = 536880547 peak = 852692992
sorting block 35, #reads = 1096738 mem = 536877133 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308428, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (266.12, 135.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 138, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (160.22, 57.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RR
[M::process] read 730528 sequences (100000064 bp) in 2.467 CPU sec, 2.632 real sec...
[M::process] read 730336 sequences (100000145 bp) in 1.319 CPU sec, 1.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309262, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 203, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (207.36, 76.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.27, 135.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731034 reads in 166.868 CPU sec, 5.324 real sec
[M::mem_process_seqs] Processed 731360 reads in 89.886 CPU sec, 2.868 real sec
sorting block 36, #reads = 1095757 mem = 536880444 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308473, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.67, 135.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730168 sequences (100000133 bp) in 2.210 CPU sec, 2.343 real sec...
[M::process] read 729872 sequences (100000128 bp) in 1.250 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308450, 3, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (267.68, 136.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 188, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (185.93, 69.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730528 reads in 172.945 CPU sec, 5.546 real sec
[M::mem_process_seqs] Processed 730336 reads in 98.395 CPU sec, 3.172 real sec
sorting block 37, #reads = 1096099 mem = 536880667 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308886, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 130, 172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288)
[M::mem_pestat] mean and std.dev: (134.20, 28.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 346)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.16, 137.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 226, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (243.60, 52.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 696)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729634 sequences (100000051 bp) in 2.072 CPU sec, 2.219 real sec...
[M::process] read 729040 sequences (100000154 bp) in 1.264 CPU sec, 1.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308421, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.81, 136.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730168 reads in 172.441 CPU sec, 5.568 real sec
[M::mem_process_seqs] Processed 729872 reads in 99.793 CPU sec, 3.191 real sec
sorting block 38, #reads = 1095464 mem = 536877767 peak = 852692992
sorting block 39, #reads = 1095443 mem = 536877968 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308336, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 157, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (183.64, 68.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 541)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (270.39, 137.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728746 sequences (100000029 bp) in 1.968 CPU sec, 2.148 real sec...
[M::process] read 728794 sequences (100000028 bp) in 1.256 CPU sec, 1.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308095, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.14, 137.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 200, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (205.54, 56.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729634 reads in 163.345 CPU sec, 5.241 real sec
[M::mem_process_seqs] Processed 729040 reads in 93.221 CPU sec, 2.988 real sec
sorting block 40, #reads = 1095293 mem = 536879380 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307649, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.47, 138.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 201, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (181.78, 65.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] skip orientation RR
[M::process] read 728946 sequences (100000092 bp) in 2.112 CPU sec, 2.252 real sec...
[M::process] read 729054 sequences (100000006 bp) in 1.150 CPU sec, 1.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307925, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 199, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (200.09, 61.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.32, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728746 reads in 168.032 CPU sec, 5.394 real sec
[M::mem_process_seqs] Processed 728794 reads in 93.634 CPU sec, 3.008 real sec
sorting block 41, #reads = 1094272 mem = 536878159 peak = 852692992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307971, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.23, 138.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728428 sequences (100000104 bp) in 2.500 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308089, 3, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (272.12, 137.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 179, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 357)
[M::mem_pestat] mean and std.dev: (166.07, 53.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] skip orientation RR
[M::process] read 728450 sequences (100000021 bp) in 1.690 CPU sec, 1.693 real sec...
[M::mem_process_seqs] Processed 728946 reads in 169.816 CPU sec, 5.424 real sec
[M::mem_process_seqs] Processed 729054 reads in 97.722 CPU sec, 3.104 real sec
sorting block 42, #reads = 1094058 mem = 536879223 peak = 852692992
sorting block 43, #reads = 1094604 mem = 536878095 peak = 863621120
[M::process] read 727598 sequences (100000138 bp) in 1.792 CPU sec, 1.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 307662, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.44, 138.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 727484 sequences (100000072 bp) in 1.400 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307888, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (274.21, 139.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 306950, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (276.89, 139.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728428 reads in 243.333 CPU sec, 7.818 real sec
[M::mem_process_seqs] Processed 728450 reads in 169.496 CPU sec, 5.462 real sec
[M::mem_process_seqs] Processed 727598 reads in 177.261 CPU sec, 5.734 real sec
sorting block 44, #reads = 1094039 mem = 536879171 peak = 863621120
[M::process] read 727550 sequences (100000211 bp) in 2.398 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307679, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (276.97, 140.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1094320 mem = 536879615 peak = 863621120
[M::process] read 727842 sequences (100000200 bp) in 1.655 CPU sec, 1.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306834, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 253, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 419)
[M::mem_pestat] mean and std.dev: (205.50, 47.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 502)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (276.26, 139.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 727484 reads in 172.034 CPU sec, 5.542 real sec
[M::mem_process_seqs] Processed 727550 reads in 98.471 CPU sec, 3.129 real sec
[M::process] read 727654 sequences (100000228 bp) in 1.336 CPU sec, 1.375 real sec...
sorting block 46, #reads = 1093081 mem = 536877111 peak = 863621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307273, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (229.53, 115.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (277.57, 140.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 727890 sequences (100000062 bp) in 1.935 CPU sec, 2.083 real sec...
[M::process] read 729002 sequences (100000122 bp) in 1.290 CPU sec, 1.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307124, 8, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (276.85, 139.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 182, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (184.23, 61.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727842 reads in 165.624 CPU sec, 5.317 real sec
[M::mem_process_seqs] Processed 727654 reads in 94.607 CPU sec, 3.030 real sec
sorting block 47, #reads = 1092847 mem = 536882048 peak = 863621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307084, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (276.39, 140.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730330 sequences (100000135 bp) in 2.358 CPU sec, 2.583 real sec...
[M::process] read 730758 sequences (100000078 bp) in 1.250 CPU sec, 1.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307738, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (272.85, 138.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 727890 reads in 165.553 CPU sec, 5.301 real sec
sorting block 48, #reads = 1093477 mem = 536878001 peak = 863621120
[M::mem_process_seqs] Processed 729002 reads in 91.113 CPU sec, 2.912 real sec
sorting block 49, #reads = 1093675 mem = 536877608 peak = 863621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 308608, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 173, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (169.11, 41.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 457)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (268.60, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732620 sequences (100000201 bp) in 1.966 CPU sec, 2.058 real sec...
[M::process] read 731850 sequences (100000079 bp) in 1.680 CPU sec, 1.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308842, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.59, 136.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730330 reads in 167.954 CPU sec, 5.367 real sec
[M::mem_process_seqs] Processed 730758 reads in 95.237 CPU sec, 3.034 real sec
sorting block 50, #reads = 1095185 mem = 536877882 peak = 863621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 309977, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 217, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (224.73, 150.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (260.56, 132.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731254 sequences (100000124 bp) in 2.072 CPU sec, 2.209 real sec...
[M::process] read 730838 sequences (100000103 bp) in 1.618 CPU sec, 1.626 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309297, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 238, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (261.67, 132.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 214, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447)
[M::mem_pestat] mean and std.dev: (204.80, 72.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 550)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732620 reads in 163.317 CPU sec, 5.215 real sec
[M::mem_process_seqs] Processed 731850 reads in 92.598 CPU sec, 2.955 real sec
sorting block 51, #reads = 1096882 mem = 536877830 peak = 863621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308996, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (263.90, 133.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731072 sequences (100000005 bp) in 2.365 CPU sec, 2.512 real sec...
[M::process] read 730242 sequences (100000205 bp) in 1.270 CPU sec, 1.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309051, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (265.70, 135.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 219, 1715)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4805)
[M::mem_pestat] mean and std.dev: (891.90, 1278.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6350)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731254 reads in 165.687 CPU sec, 5.292 real sec
sorting block 52, #reads = 1096552 mem = 536880930 peak = 863621120
[M::mem_process_seqs] Processed 730838 reads in 91.039 CPU sec, 2.914 real sec
sorting block 53, #reads = 1096487 mem = 536878462 peak = 871456768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308680, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (265.87, 135.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 157, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (222.55, 122.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] skip orientation RR
[M::process] read 730216 sequences (100000104 bp) in 2.130 CPU sec, 2.276 real sec...
[M::process] read 729798 sequences (100000170 bp) in 1.620 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308801, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 134, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (149.64, 56.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 563)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (268.11, 135.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 731072 reads in 174.424 CPU sec, 5.583 real sec
[M::mem_process_seqs] Processed 730242 reads in 98.532 CPU sec, 3.148 real sec
sorting block 54, #reads = 1095818 mem = 536879662 peak = 871456768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308814, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (267.06, 135.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729822 sequences (100000054 bp) in 2.259 CPU sec, 2.370 real sec...
[M::process] read 729478 sequences (100000004 bp) in 1.660 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308293, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (268.99, 136.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730216 reads in 171.778 CPU sec, 5.485 real sec
[M::mem_process_seqs] Processed 729798 reads in 100.368 CPU sec, 3.203 real sec
sorting block 55, #reads = 1095729 mem = 536877046 peak = 871456768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308071, 8, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (270.02, 137.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 307, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (227.67, 80.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation RR
[M::process] read 729098 sequences (100000059 bp) in 2.390 CPU sec, 2.535 real sec...
[M::process] read 728690 sequences (100000128 bp) in 1.680 CPU sec, 1.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308152, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (271.55, 138.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729822 reads in 173.671 CPU sec, 5.556 real sec
sorting block 56, #reads = 1095167 mem = 536879272 peak = 871456768
[M::mem_process_seqs] Processed 729478 reads in 102.714 CPU sec, 3.275 real sec
sorting block 57, #reads = 1094882 mem = 536879200 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308348, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.12, 137.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 728922 sequences (100000075 bp) in 2.442 CPU sec, 2.596 real sec...
[M::process] read 729044 sequences (100000018 bp) in 1.605 CPU sec, 1.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308275, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.98, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 182, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (174.22, 74.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729098 reads in 170.154 CPU sec, 5.422 real sec
[M::mem_process_seqs] Processed 728690 reads in 96.043 CPU sec, 3.049 real sec
sorting block 58, #reads = 1095155 mem = 536878111 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308158, 1, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 187, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (228.43, 111.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.23, 137.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 267, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (266.73, 93.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728402 sequences (100000209 bp) in 2.314 CPU sec, 2.456 real sec...
[M::process] read 727936 sequences (100000028 bp) in 1.788 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308091, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 184, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (194.27, 82.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (271.31, 137.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 174, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (184.11, 72.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 477)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728922 reads in 172.982 CPU sec, 5.533 real sec
[M::mem_process_seqs] Processed 729044 reads in 98.246 CPU sec, 3.122 real sec
sorting block 59, #reads = 1094508 mem = 536881499 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307631, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 189, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (194.82, 64.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (272.10, 136.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 727460 sequences (100000247 bp) in 2.005 CPU sec, 2.200 real sec...
[M::process] read 726800 sequences (100000196 bp) in 1.310 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307432, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (274.08, 138.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728402 reads in 170.880 CPU sec, 5.472 real sec
sorting block 60, #reads = 1094142 mem = 536880383 peak = 872669184
[M::mem_process_seqs] Processed 727936 reads in 95.726 CPU sec, 3.072 real sec
sorting block 61, #reads = 1093650 mem = 536878111 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307425, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 163, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (189.57, 71.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (276.72, 139.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726890 sequences (100000097 bp) in 2.430 CPU sec, 2.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307071, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 257, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (278.12, 138.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 727460 reads in 172.258 CPU sec, 5.516 real sec
[M::process] read 726202 sequences (100000241 bp) in 1.717 CPU sec, 1.718 real sec...
[M::mem_process_seqs] Processed 726800 reads in 99.437 CPU sec, 3.155 real sec
sorting block 62, #reads = 1093309 mem = 536878213 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307283, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 184, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (190.20, 55.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 610)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (279.41, 140.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726090 sequences (100000027 bp) in 2.146 CPU sec, 2.375 real sec...
[M::process] read 726184 sequences (100000036 bp) in 1.282 CPU sec, 1.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306680, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (280.62, 140.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 726890 reads in 163.959 CPU sec, 5.247 real sec
[M::mem_process_seqs] Processed 726202 reads in 92.317 CPU sec, 2.945 real sec
sorting block 63, #reads = 1092804 mem = 536882254 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306956, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (280.05, 139.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 693, 706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2114)
[M::mem_pestat] mean and std.dev: (273.50, 331.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2818)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 64, #reads = 1092233 mem = 536879581 peak = 872669184
[M::process] read 725860 sequences (100000121 bp) in 2.580 CPU sec, 2.763 real sec...
[M::process] read 728080 sequences (100000107 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306211, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 140, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (172.10, 65.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 258, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (279.58, 139.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 150, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (185.94, 86.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726090 reads in 172.650 CPU sec, 5.527 real sec
[M::mem_process_seqs] Processed 726184 reads in 96.987 CPU sec, 3.087 real sec
sorting block 65, #reads = 1091893 mem = 536879612 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306202, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 230, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (191.67, 60.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (281.84, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728768 sequences (100000063 bp) in 1.866 CPU sec, 2.007 real sec...
[M::process] read 730392 sequences (100000241 bp) in 1.300 CPU sec, 1.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307615, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 167, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (181.55, 62.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (274.53, 138.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 725860 reads in 165.980 CPU sec, 5.316 real sec
[M::mem_process_seqs] Processed 728080 reads in 91.190 CPU sec, 2.922 real sec
sorting block 66, #reads = 1091382 mem = 536880730 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308003, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (272.13, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 146, 181)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 279)
[M::mem_pestat] mean and std.dev: (154.64, 37.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 328)
[M::mem_pestat] skip orientation RR
[M::process] read 733944 sequences (100000137 bp) in 2.187 CPU sec, 2.344 real sec...
[M::process] read 733194 sequences (100000039 bp) in 1.470 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308747, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (268.07, 136.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 203, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (224.20, 65.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728768 reads in 164.181 CPU sec, 5.254 real sec
[M::mem_process_seqs] Processed 730392 reads in 90.837 CPU sec, 2.897 real sec
sorting block 67, #reads = 1093921 mem = 536877667 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309867, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 233, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (260.38, 136.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1094241 mem = 536877186 peak = 872669184
[M::process] read 733036 sequences (100000198 bp) in 2.226 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309699, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 237, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (263.40, 137.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 203, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 468)
[M::mem_pestat] mean and std.dev: (207.20, 57.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RR
[M::process] read 732460 sequences (100000139 bp) in 1.697 CPU sec, 1.701 real sec...
[M::mem_process_seqs] Processed 733944 reads in 171.621 CPU sec, 5.502 real sec
[M::mem_process_seqs] Processed 733194 reads in 99.046 CPU sec, 3.144 real sec
sorting block 69, #reads = 1098052 mem = 536879553 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309671, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 174, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 271)
[M::mem_pestat] mean and std.dev: (166.80, 41.06)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 331)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (263.33, 137.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 146, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 210)
[M::mem_pestat] mean and std.dev: (138.90, 22.81)
[M::mem_pestat] low and high boundaries for proper pairs: (48, 236)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 731830 sequences (100000146 bp) in 2.485 CPU sec, 2.643 real sec...
[M::process] read 731714 sequences (100000235 bp) in 1.395 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309415, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 192, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407)
[M::mem_pestat] mean and std.dev: (202.50, 55.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (264.09, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 733036 reads in 169.040 CPU sec, 5.399 real sec
[M::mem_process_seqs] Processed 732460 reads in 94.846 CPU sec, 3.025 real sec
sorting block 70, #reads = 1097680 mem = 536881571 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 309521, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 247, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (286.13, 118.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (265.57, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731424 sequences (100000243 bp) in 1.974 CPU sec, 2.117 real sec...
[M::process] read 732410 sequences (100000200 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309255, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (266.09, 137.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731830 reads in 170.921 CPU sec, 5.470 real sec
[M::mem_process_seqs] Processed 731714 reads in 96.653 CPU sec, 3.086 real sec
sorting block 71, #reads = 1097155 mem = 536879344 peak = 872669184
sorting block 72, #reads = 1097105 mem = 536877945 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308922, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 176, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (157.67, 49.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (265.34, 136.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732634 sequences (100000083 bp) in 2.435 CPU sec, 2.570 real sec...
[M::process] read 732190 sequences (100000161 bp) in 1.823 CPU sec, 1.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309546, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (262.22, 134.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 172, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 283)
[M::mem_pestat] mean and std.dev: (161.62, 42.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 330)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731424 reads in 173.487 CPU sec, 5.525 real sec
[M::mem_process_seqs] Processed 732410 reads in 98.910 CPU sec, 3.130 real sec
sorting block 73, #reads = 1096617 mem = 536878574 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309883, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 175, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (154.82, 44.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 474)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (261.27, 134.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732258 sequences (100000153 bp) in 2.541 CPU sec, 2.685 real sec...
[M::process] read 732068 sequences (100000106 bp) in 1.413 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309669, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 243, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (237.40, 97.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 237, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (260.91, 132.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 231, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (157.00, 72.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732634 reads in 168.618 CPU sec, 5.379 real sec
[M::mem_process_seqs] Processed 732190 reads in 94.272 CPU sec, 3.006 real sec
sorting block 74, #reads = 1097873 mem = 536878734 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309754, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 236, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (259.70, 132.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732058 sequences (100000279 bp) in 1.899 CPU sec, 2.068 real sec...
[M::process] read 732676 sequences (100000249 bp) in 1.740 CPU sec, 1.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309565, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 175, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 292)
[M::mem_pestat] mean and std.dev: (164.50, 46.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 350)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (261.98, 134.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 179, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (194.30, 77.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732258 reads in 173.257 CPU sec, 5.547 real sec
[M::mem_process_seqs] Processed 732068 reads in 97.126 CPU sec, 3.090 real sec
sorting block 75, #reads = 1096844 mem = 536878538 peak = 872669184
sorting block 76, #reads = 1097246 mem = 536877152 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309501, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 237, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (261.74, 133.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732304 sequences (100000225 bp) in 2.282 CPU sec, 2.394 real sec...
[M::process] read 731916 sequences (100000002 bp) in 1.780 CPU sec, 1.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309664, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (260.65, 133.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732058 reads in 166.594 CPU sec, 5.305 real sec
[M::mem_process_seqs] Processed 732676 reads in 95.664 CPU sec, 3.025 real sec
sorting block 77, #reads = 1096761 mem = 536878626 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309778, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 256, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 428)
[M::mem_pestat] mean and std.dev: (211.27, 56.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (262.12, 134.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 731512 sequences (100000235 bp) in 2.469 CPU sec, 2.663 real sec...
[M::process] read 731346 sequences (100000072 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309153, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 238, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (262.56, 134.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732304 reads in 164.880 CPU sec, 5.254 real sec
[M::mem_process_seqs] Processed 731916 reads in 91.959 CPU sec, 2.925 real sec
sorting block 78, #reads = 1097144 mem = 536878638 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308923, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (263.62, 135.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731684 sequences (100000052 bp) in 2.841 CPU sec, 3.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309234, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.72, 134.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731512 reads in 167.295 CPU sec, 5.337 real sec
[M::mem_process_seqs] Processed 731346 reads in 93.053 CPU sec, 2.947 real sec
[M::process] read 730702 sequences (100000093 bp) in 1.763 CPU sec, 1.791 real sec...
sorting block 79, #reads = 1096616 mem = 536879099 peak = 872669184
sorting block 80, #reads = 1095171 mem = 536877459 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309046, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 185, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (187.64, 50.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 473)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (263.60, 134.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730430 sequences (100000072 bp) in 2.231 CPU sec, 2.382 real sec...
[M::process] read 730292 sequences (100000056 bp) in 1.620 CPU sec, 1.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308493, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (265.89, 135.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731684 reads in 171.747 CPU sec, 5.484 real sec
[M::mem_process_seqs] Processed 730702 reads in 95.903 CPU sec, 3.055 real sec
sorting block 81, #reads = 1096232 mem = 536877305 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308650, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 192, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373)
[M::mem_pestat] mean and std.dev: (180.00, 54.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (267.23, 136.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730432 sequences (100000173 bp) in 2.491 CPU sec, 2.640 real sec...
[M::process] read 728212 sequences (100000001 bp) in 1.560 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308369, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 256, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (264.30, 146.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.98, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 167, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 353)
[M::mem_pestat] mean and std.dev: (184.36, 58.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 417)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730430 reads in 163.440 CPU sec, 5.200 real sec
[M::mem_process_seqs] Processed 730292 reads in 89.903 CPU sec, 2.858 real sec
sorting block 82, #reads = 1095307 mem = 536878494 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308734, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.99, 136.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 157, 162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (75, 220)
[M::mem_pestat] mean and std.dev: (152.36, 23.65)
[M::mem_pestat] low and high boundaries for proper pairs: (46, 249)
[M::mem_pestat] skip orientation RR
[M::process] read 733104 sequences (100000068 bp) in 2.539 CPU sec, 2.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307687, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (276.18, 141.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730432 reads in 169.434 CPU sec, 5.428 real sec
[M::mem_process_seqs] Processed 728212 reads in 95.136 CPU sec, 3.016 real sec
[M::process] read 733800 sequences (100000281 bp) in 2.009 CPU sec, 2.061 real sec...
sorting block 83, #reads = 1095961 mem = 536877956 peak = 872669184
sorting block 84, #reads = 1094360 mem = 536877143 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309668, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 236, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (262.58, 136.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 243, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (195.55, 65.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RR
[M::process] read 732724 sequences (100000219 bp) in 2.314 CPU sec, 2.426 real sec...
[M::process] read 732552 sequences (100000062 bp) in 1.376 CPU sec, 1.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 309778, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 235, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (261.23, 136.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 733104 reads in 172.910 CPU sec, 5.534 real sec
[M::mem_process_seqs] Processed 733800 reads in 102.024 CPU sec, 3.260 real sec
sorting block 85, #reads = 1096717 mem = 536878207 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309785, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (263.13, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732240 sequences (100000015 bp) in 2.097 CPU sec, 2.266 real sec...
[M::process] read 732076 sequences (100000144 bp) in 1.590 CPU sec, 1.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309227, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (264.53, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 263, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (254.00, 133.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 732724 reads in 169.420 CPU sec, 5.424 real sec
[M::mem_process_seqs] Processed 732552 reads in 97.565 CPU sec, 3.118 real sec
sorting block 86, #reads = 1097928 mem = 536878127 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309197, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (263.99, 136.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 197, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (196.38, 40.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RR
[M::process] read 731684 sequences (100000071 bp) in 2.243 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309404, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 240, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (264.42, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731058 sequences (100000060 bp) in 1.870 CPU sec, 1.863 real sec...
[M::mem_process_seqs] Processed 732240 reads in 168.798 CPU sec, 5.390 real sec
[M::mem_process_seqs] Processed 732076 reads in 97.071 CPU sec, 3.075 real sec
sorting block 87, #reads = 1096936 mem = 536878053 peak = 872669184
sorting block 88, #reads = 1096772 mem = 536879501 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 309123, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (265.95, 136.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731322 sequences (100000135 bp) in 2.056 CPU sec, 2.203 real sec...
[M::process] read 730710 sequences (100000203 bp) in 1.260 CPU sec, 1.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308757, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.28, 137.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 731684 reads in 173.422 CPU sec, 5.562 real sec
[M::mem_process_seqs] Processed 731058 reads in 97.825 CPU sec, 3.133 real sec
sorting block 89, #reads = 1096792 mem = 536878727 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308970, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.05, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730558 sequences (100000094 bp) in 1.996 CPU sec, 2.208 real sec...
[M::process] read 730878 sequences (100000129 bp) in 1.310 CPU sec, 1.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308509, 1, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (268.52, 138.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 174, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (181.38, 48.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731322 reads in 164.093 CPU sec, 5.256 real sec
[M::mem_process_seqs] Processed 730710 reads in 91.762 CPU sec, 2.941 real sec
sorting block 90, #reads = 1096137 mem = 536878155 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308406, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 190, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (173.55, 75.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (266.31, 135.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730558 sequences (100000266 bp) in 2.161 CPU sec, 2.328 real sec...
[M::process] read 730752 sequences (100000070 bp) in 1.260 CPU sec, 1.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308639, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (265.80, 135.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730558 reads in 168.376 CPU sec, 5.391 real sec
[M::mem_process_seqs] Processed 730878 reads in 91.937 CPU sec, 2.929 real sec
sorting block 91, #reads = 1095806 mem = 536879043 peak = 872669184
sorting block 92, #reads = 1095903 mem = 536877857 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308647, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (267.91, 137.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730990 sequences (100000072 bp) in 1.970 CPU sec, 2.114 real sec...
[M::process] read 730818 sequences (100000276 bp) in 1.760 CPU sec, 1.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308244, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.82, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730558 reads in 170.099 CPU sec, 5.437 real sec
[M::mem_process_seqs] Processed 730752 reads in 97.978 CPU sec, 3.113 real sec
sorting block 93, #reads = 1095500 mem = 536880007 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308882, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.11, 137.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 186, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429)
[M::mem_pestat] mean and std.dev: (164.67, 54.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 534)
[M::mem_pestat] skip orientation RR
[M::process] read 730562 sequences (100000030 bp) in 2.320 CPU sec, 2.439 real sec...
[M::process] read 730132 sequences (100000052 bp) in 1.730 CPU sec, 1.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308836, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (267.37, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 237, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 372)
[M::mem_pestat] mean and std.dev: (216.50, 42.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 436)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730990 reads in 164.131 CPU sec, 5.226 real sec
[M::mem_process_seqs] Processed 730818 reads in 90.704 CPU sec, 2.884 real sec
sorting block 94, #reads = 1095574 mem = 536879650 peak = 872669184
[M::process] read 730624 sequences (100000100 bp) in 2.961 CPU sec, 3.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308323, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (269.23, 138.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 218, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (191.00, 62.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730132 reads in 82.952 CPU sec, 2.616 real sec
[M::process] read 730920 sequences (100000252 bp) in 1.577 CPU sec, 1.592 real sec...
sorting block 95, #reads = 1096051 mem = 536880091 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 308310, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (268.29, 137.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308798, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 145, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (165.55, 77.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.57, 137.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730562 reads in 249.474 CPU sec, 7.969 real sec
[M::mem_process_seqs] Processed 730624 reads in 102.956 CPU sec, 3.310 real sec
[M::process] read 730396 sequences (100000042 bp) in 1.631 CPU sec, 1.693 real sec...
sorting block 96, #reads = 1095577 mem = 536876348 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308905, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (266.39, 137.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 246, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (151, 356)
[M::mem_pestat] mean and std.dev: (249.83, 12.23)
[M::mem_pestat] low and high boundaries for proper pairs: (110, 397)
[M::mem_pestat] skip orientation RR
[M::process] read 729888 sequences (100000191 bp) in 2.369 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308320, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (269.77, 139.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730920 reads in 162.140 CPU sec, 5.201 real sec
[M::process] read 730264 sequences (100000037 bp) in 1.413 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 730396 reads in 91.354 CPU sec, 2.905 real sec
sorting block 97, #reads = 1095802 mem = 536878309 peak = 872669184
sorting block 98, #reads = 1095715 mem = 536877734 peak = 872669184
[M::process] read 731230 sequences (100000004 bp) in 2.476 CPU sec, 2.615 real sec...
[M::process] read 731356 sequences (100000004 bp) in 1.420 CPU sec, 1.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308887, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 189, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 342)
[M::mem_pestat] mean and std.dev: (193.10, 56.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 420)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (269.97, 139.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 730264 reads in 86.315 CPU sec, 2.743 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 308150, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 140, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416)
[M::mem_pestat] mean and std.dev: (155.15, 65.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (271.45, 140.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 309008, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (265.61, 136.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 735284 sequences (100000268 bp) in 1.553 CPU sec, 1.561 real sec...
[M::mem_process_seqs] Processed 729888 reads in 244.877 CPU sec, 7.834 real sec
sorting block 99, #reads = 1095194 mem = 536878521 peak = 872669184
[M::mem_process_seqs] Processed 731230 reads in 108.915 CPU sec, 3.541 real sec
sorting block 100, #reads = 1095312 mem = 536879328 peak = 872669184
[M::process] read 733410 sequences (100000029 bp) in 2.602 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 310574, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 261, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (225.60, 54.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 229, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (255.79, 133.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 735284 reads in 89.494 CPU sec, 2.834 real sec
[M::process] read 732442 sequences (100000274 bp) in 2.001 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309219, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (266.36, 138.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 175, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (168.90, 52.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 553)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309837, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 206, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (189.64, 68.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 235, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (260.03, 133.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 232, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 348)
[M::mem_pestat] mean and std.dev: (224.09, 30.99)
[M::mem_pestat] low and high boundaries for proper pairs: (54, 397)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 732228 sequences (100000190 bp) in 1.133 CPU sec, 1.142 real sec...
[M::mem_process_seqs] Processed 731356 reads in 251.802 CPU sec, 8.063 real sec
sorting block 101, #reads = 1098467 mem = 536877030 peak = 872669184
[M::mem_process_seqs] Processed 733410 reads in 107.292 CPU sec, 3.476 real sec
sorting block 102, #reads = 1096718 mem = 536877931 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309493, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 168, 1089)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2935)
[M::mem_pestat] mean and std.dev: (491.18, 562.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3858)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (262.74, 135.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 732008 sequences (100000113 bp) in 2.293 CPU sec, 2.468 real sec...
[M::process] read 731630 sequences (100000282 bp) in 1.260 CPU sec, 1.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 309559, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (264.04, 136.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732442 reads in 166.016 CPU sec, 5.314 real sec
[M::mem_process_seqs] Processed 732228 reads in 94.569 CPU sec, 3.037 real sec
sorting block 103, #reads = 1097282 mem = 536878938 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308988, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (264.61, 136.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 731060 sequences (100000043 bp) in 2.889 CPU sec, 3.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 309233, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (265.57, 136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 732008 reads in 168.324 CPU sec, 5.426 real sec
[M::mem_process_seqs] Processed 731630 reads in 108.100 CPU sec, 3.464 real sec
sorting block 104, #reads = 1095466 mem = 536877562 peak = 872669184
[M::process] read 730876 sequences (100000139 bp) in 3.181 CPU sec, 3.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 308777, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.08, 137.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308706, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.30, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 191, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724)
[M::mem_pestat] mean and std.dev: (326.69, 249.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2249)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731060 reads in 162.375 CPU sec, 5.249 real sec
[M::process] read 731162 sequences (100000057 bp) in 2.752 CPU sec, 3.043 real sec...
[M::mem_process_seqs] Processed 730876 reads in 98.352 CPU sec, 3.162 real sec
sorting block 105, #reads = 1096626 mem = 536880104 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 308712, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (266.75, 137.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 167, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (220.18, 101.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RR
[M::process] read 730748 sequences (100000072 bp) in 2.682 CPU sec, 2.903 real sec...
[M::mem_process_seqs] Processed 731162 reads in 86.135 CPU sec, 2.892 real sec
sorting block 106, #reads = 1096276 mem = 536879873 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 309087, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 151, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 295)
[M::mem_pestat] mean and std.dev: (146.45, 42.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 350)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.58, 137.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730240 sequences (100000241 bp) in 2.734 CPU sec, 2.949 real sec...
[M::mem_process_seqs] Processed 730748 reads in 85.142 CPU sec, 2.820 real sec
sorting block 107, #reads = 1096210 mem = 536880343 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308278, 10, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 152, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (162.50, 67.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (268.14, 136.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (52, 65, 108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220)
[M::mem_pestat] mean and std.dev: (63.25, 21.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 276)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 730240 reads in 84.002 CPU sec, 2.780 real sec
[M::process] read 730192 sequences (100000083 bp) in 2.934 CPU sec, 3.214 real sec...
sorting block 108, #reads = 1095895 mem = 536878256 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308144, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 144, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (187.00, 75.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 672)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (269.81, 138.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 296, 1835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5207)
[M::mem_pestat] mean and std.dev: (764.82, 713.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6893)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730192 reads in 91.461 CPU sec, 2.974 real sec
[M::process] read 729906 sequences (100000242 bp) in 2.792 CPU sec, 3.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308418, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (271.21, 138.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 730154 sequences (100000117 bp) in 2.489 CPU sec, 2.761 real sec...
[M::mem_process_seqs] Processed 729906 reads in 85.820 CPU sec, 2.784 real sec
sorting block 109, #reads = 1095319 mem = 536877974 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308341, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 246, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (270.25, 138.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 729950 sequences (100000137 bp) in 2.522 CPU sec, 2.766 real sec...
[M::mem_process_seqs] Processed 730154 reads in 88.041 CPU sec, 2.866 real sec
sorting block 110, #reads = 1095018 mem = 536879238 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 308272, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (99, 161, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408)
[M::mem_pestat] mean and std.dev: (137.75, 41.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (270.78, 138.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 729398 sequences (100000264 bp) in 2.750 CPU sec, 2.950 real sec...
[M::mem_process_seqs] Processed 729950 reads in 93.430 CPU sec, 3.059 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307745, 7, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (271.96, 139.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 126, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (184.69, 116.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729398 reads in 86.880 CPU sec, 2.837 real sec
sorting block 111, #reads = 1095090 mem = 536878140 peak = 872669184
[M::process] read 728674 sequences (100000023 bp) in 3.561 CPU sec, 3.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 307443, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (275.18, 140.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 255, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (310.36, 196.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728674 reads in 86.386 CPU sec, 2.821 real sec
[M::process] read 728504 sequences (100000216 bp) in 3.380 CPU sec, 3.850 real sec...
sorting block 112, #reads = 1094728 mem = 536878053 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307765, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (276.55, 141.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728504 reads in 95.560 CPU sec, 3.116 real sec
[M::process] read 728850 sequences (100000268 bp) in 3.292 CPU sec, 3.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307440, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (275.01, 141.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728850 reads in 94.520 CPU sec, 3.091 real sec
[M::process] read 729084 sequences (100000012 bp) in 3.102 CPU sec, 3.461 real sec...
sorting block 113, #reads = 1093854 mem = 536883601 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 307556, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (274.89, 141.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729084 reads in 97.276 CPU sec, 3.183 real sec
[M::process] read 728146 sequences (100000276 bp) in 2.957 CPU sec, 3.251 real sec...
sorting block 114, #reads = 1094150 mem = 536876286 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307389, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 161, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (169.09, 69.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (276.82, 142.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728146 reads in 94.432 CPU sec, 3.108 real sec
[M::process] read 728778 sequences (100000185 bp) in 3.423 CPU sec, 3.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307705, 9, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 180, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (193.75, 75.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (276.53, 143.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 728778 reads in 91.556 CPU sec, 2.982 real sec
sorting block 115, #reads = 1093907 mem = 536879453 peak = 872669184
[M::process] read 730020 sequences (100000283 bp) in 3.428 CPU sec, 3.783 real sec...
[M::process] read 731126 sequences (100000088 bp) in 2.092 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 308044, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (271.78, 140.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 256, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (200.00, 82.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 675)
[M::mem_pestat] skip orientation RR
[M::process] read 732366 sequences (100000052 bp) in 1.350 CPU sec, 1.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308745, 3, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 242, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.24, 138.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 178, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (200.85, 80.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] skip orientation RR
[M::process] read 734670 sequences (100000179 bp) in 1.340 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 730020 reads in 157.255 CPU sec, 4.973 real sec
[M::mem_process_seqs] Processed 731126 reads in 97.919 CPU sec, 3.128 real sec
sorting block 116, #reads = 1094091 mem = 536879799 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 310673, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 231, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (257.28, 133.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732814 sequences (100000064 bp) in 3.179 CPU sec, 3.572 real sec...
sorting block 117, #reads = 1095722 mem = 536878117 peak = 872669184
[M::mem_process_seqs] Processed 734670 reads in 82.905 CPU sec, 2.712 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309391, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (264.71, 138.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 732462 sequences (100000030 bp) in 1.746 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309442, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 157, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (167.82, 61.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 631)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 237, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (261.97, 134.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732366 reads in 231.542 CPU sec, 7.491 real sec
[M::mem_process_seqs] Processed 732814 reads in 91.116 CPU sec, 2.922 real sec
sorting block 118, #reads = 1097686 mem = 536877874 peak = 872669184
[M::process] read 732268 sequences (100000213 bp) in 1.889 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 309238, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 238, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (263.70, 136.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 144, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (161.80, 73.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] skip orientation RR
sorting block 119, #reads = 1097466 mem = 536878391 peak = 872669184
[M::process] read 731554 sequences (100000193 bp) in 2.196 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 309660, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (206.00, 91.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (264.33, 136.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 732462 reads in 142.527 CPU sec, 4.564 real sec
[M::mem_process_seqs] Processed 732268 reads in 84.653 CPU sec, 2.707 real sec
[M::process] read 731594 sequences (100000145 bp) in 2.257 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 308709, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 213, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (193.58, 59.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 241, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (265.76, 136.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 173, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 361)
[M::mem_pestat] mean and std.dev: (162.10, 44.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 120, #reads = 1096920 mem = 536880731 peak = 872669184
[M::process] read 731096 sequences (100000155 bp) in 2.351 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 309138, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 176, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (174.38, 48.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 242, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (266.81, 137.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 174, 871)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345)
[M::mem_pestat] mean and std.dev: (389.50, 368.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3082)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731554 reads in 141.459 CPU sec, 4.532 real sec
[M::mem_process_seqs] Processed 731594 reads in 85.032 CPU sec, 2.713 real sec
[M::process] read 730570 sequences (100000054 bp) in 2.322 CPU sec, 2.494 real sec...
sorting block 121, #reads = 1095510 mem = 536877240 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 308384, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 243, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.51, 137.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 198, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (190.31, 55.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 482)
[M::mem_pestat] skip orientation RR
[M::process] read 730642 sequences (100000001 bp) in 2.133 CPU sec, 2.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 308384, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (268.38, 137.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 159, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346)
[M::mem_pestat] mean and std.dev: (154.67, 62.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 731096 reads in 143.098 CPU sec, 4.603 real sec
[M::mem_process_seqs] Processed 730570 reads in 87.849 CPU sec, 2.807 real sec
sorting block 122, #reads = 1095893 mem = 536881006 peak = 872669184
[M::process] read 729952 sequences (100000091 bp) in 2.482 CPU sec, 2.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308408, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 231, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (209.09, 66.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 553)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (268.89, 138.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 123, #reads = 1095910 mem = 536881999 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 308342, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 229, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (201.50, 67.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (269.87, 137.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 730098 sequences (100000141 bp) in 2.304 CPU sec, 2.503 real sec...
[M::mem_process_seqs] Processed 730642 reads in 138.468 CPU sec, 4.469 real sec
[M::mem_process_seqs] Processed 729952 reads in 83.396 CPU sec, 2.677 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 308267, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 172, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (169.25, 64.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 245, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (269.32, 137.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 176, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 313)
[M::mem_pestat] mean and std.dev: (155.00, 29.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 372)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 730098 reads in 74.169 CPU sec, 2.484 real sec
[M::process] read 729480 sequences (100000282 bp) in 2.714 CPU sec, 3.022 real sec...
sorting block 124, #reads = 1095481 mem = 536879634 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 307972, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 247, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (271.33, 138.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 180, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2037)
[M::mem_pestat] mean and std.dev: (344.00, 284.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2664)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729480 reads in 73.175 CPU sec, 2.448 real sec
[M::process] read 729470 sequences (100000119 bp) in 2.699 CPU sec, 2.978 real sec...
sorting block 125, #reads = 1095313 mem = 536877140 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 307907, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 246, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (269.90, 137.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 216, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (230.83, 73.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729470 reads in 73.148 CPU sec, 2.438 real sec
[M::process] read 729148 sequences (100000177 bp) in 2.796 CPU sec, 3.134 real sec...
sorting block 126, #reads = 1094567 mem = 536879793 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307429, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (272.27, 138.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 729148 reads in 74.685 CPU sec, 2.464 real sec
[M::process] read 729076 sequences (100000168 bp) in 2.685 CPU sec, 2.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 307440, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (271.70, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 127, #reads = 1094505 mem = 536878941 peak = 872669184
[M::mem_process_seqs] Processed 729076 reads in 78.075 CPU sec, 2.551 real sec
[M::process] read 728470 sequences (100000029 bp) in 2.573 CPU sec, 2.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 307331, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 250, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (273.36, 138.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 728470 reads in 75.582 CPU sec, 2.473 real sec
[M::process] read 728066 sequences (100000002 bp) in 3.088 CPU sec, 3.540 real sec...
sorting block 128, #reads = 1094167 mem = 536881169 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306947, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 206, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 319)
[M::mem_pestat] mean and std.dev: (184.22, 30.75)
[M::mem_pestat] low and high boundaries for proper pairs: (19, 369)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (274.99, 138.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 223, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (249.40, 153.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728066 reads in 76.755 CPU sec, 2.510 real sec
[M::process] read 727596 sequences (100000185 bp) in 3.116 CPU sec, 3.495 real sec...
sorting block 129, #reads = 1093399 mem = 536879042 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306574, 3, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (276.81, 139.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 198, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (231.92, 127.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727596 reads in 77.295 CPU sec, 2.514 real sec
[M::process] read 727188 sequences (100000020 bp) in 2.605 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306856, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (277.67, 139.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 184, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (152.25, 59.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RR
[M::process] read 727706 sequences (100000152 bp) in 2.135 CPU sec, 2.327 real sec...
[M::mem_process_seqs] Processed 727188 reads in 77.726 CPU sec, 2.525 real sec
sorting block 130, #reads = 1092819 mem = 536879886 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307130, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 226, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (224.71, 70.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (276.33, 139.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 267, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187)
[M::mem_pestat] mean and std.dev: (328.33, 258.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1538)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727706 reads in 79.589 CPU sec, 2.592 real sec
[M::process] read 727336 sequences (100000037 bp) in 2.620 CPU sec, 2.850 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306476, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 160, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (177.36, 46.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 591)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (277.32, 139.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 283, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 541)
[M::mem_pestat] mean and std.dev: (270.36, 63.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727336 reads in 83.165 CPU sec, 2.736 real sec
sorting block 131, #reads = 1092646 mem = 536878799 peak = 872669184
[M::process] read 727532 sequences (100000241 bp) in 2.861 CPU sec, 3.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307149, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (277.79, 140.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 277, 826)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034)
[M::mem_pestat] mean and std.dev: (498.07, 468.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2638)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727532 reads in 84.416 CPU sec, 2.773 real sec
[M::process] read 728422 sequences (100000229 bp) in 3.632 CPU sec, 4.083 real sec...
sorting block 132, #reads = 1093343 mem = 536879513 peak = 872669184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307455, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.37, 140.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 216, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1608)
[M::mem_pestat] mean and std.dev: (394.73, 272.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2077)
[M::mem_pestat] skip orientation RR
[M::process] read 730138 sequences (100000274 bp) in 2.515 CPU sec, 2.763 real sec...
[M::mem_process_seqs] Processed 728422 reads in 86.764 CPU sec, 2.830 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 308251, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 187, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (185.50, 74.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (268.39, 137.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 267, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (250.67, 73.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 768)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 133, #reads = 1093090 mem = 536884782 peak = 872669184
[M::mem_process_seqs] Processed 730138 reads in 84.561 CPU sec, 2.764 real sec
[M::process] read 730114 sequences (100000205 bp) in 2.663 CPU sec, 2.942 real sec...
[M::process] read 25028 sequences (3429589 bp) in 0.324 CPU sec, 0.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 308012, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 244, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (269.30, 138.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 10569, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (267.76, 139.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 730114 reads in 84.721 CPU sec, 2.769 real sec
[M::mem_process_seqs] Processed 25028 reads in 17.690 CPU sec, 0.595 real sec
sorting block 134, #reads = 1094915 mem = 536881243 peak = 872669184
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 147544668
overall: 69608321024 mem 19701.565 user 1638.258 sys 743.606 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.1_25PercentHG514.L004\tPU:L004\tSM:1_25PercentHG514\tLB:1_25PercentHG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1_25PercentHG514_R1.fastq.gz 0002.1_25PercentHG514_R2.fastq.gz
[main] Real time: 743.644 sec; CPU: 21339.825 sec
sorting block 135, #reads = 499389 mem = 244982977 peak = 872669184
uniq header count 1
merging 136 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 6527166398
output reads: 148378690
bam_mem_sort: 136 calls 87.576 user 1.074 sys 88.930 real
bam_write: 136 calls 53.588 user 2.850 sys 57.416 real
execute: 1 calls 27.079 user 1.604 sys 775.855 real
merge_files: 1 calls 27.046 user 1.582 sys 31.786 real
parse_chunk: 2168 calls 87.396 user 24.390 sys 113.652 real
read_chunk: 5876 calls 58.384 user 47.488 sys 614.986 real
sort_block: 1 calls 46.135 user 46.632 sys 597.043 real
write_chunk: 3706 calls 26.712 user 1.203 sys 30.664 real
overall: 2351656960 mem 1411.847 user 99.374 sys 775.878 real
ls: cannot access '*.cram': No such file or directory