cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o D0001483_22q.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1925.501 CPU sec, 68.893 real sec
[M::process] read 717256 sequences (100000165 bp) in 1.017 CPU sec, 1.020 real sec...
[M::process] read 716184 sequences (100000037 bp) in 1.307 CPU sec, 1.318 real sec...
[M::process] read 716214 sequences (100000155 bp) in 1.304 CPU sec, 1.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304538, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 318)
[M::mem_pestat] mean and std.dev: (165.53, 47.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 375)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715724 sequences (100000118 bp) in 1.189 CPU sec, 1.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304601, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 355)
[M::mem_pestat] mean and std.dev: (178.50, 63.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 433)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304920, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (183.35, 69.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 717256 reads in 266.247 CPU sec, 8.676 real sec
[M::mem_process_seqs] Processed 716184 reads in 166.896 CPU sec, 5.544 real sec
[M::mem_process_seqs] Processed 716214 reads in 180.982 CPU sec, 6.042 real sec
sorting block 0, #reads = 1093252 mem = 536879215 peak = 584323072
[M::process] read 715660 sequences (100000252 bp) in 1.428 CPU sec, 1.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304544, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (183.61, 69.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715820 sequences (100000111 bp) in 1.441 CPU sec, 1.546 real sec...
[M::process] read 715762 sequences (100000149 bp) in 1.050 CPU sec, 1.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304258, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (186.45, 72.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715724 reads in 156.903 CPU sec, 5.030 real sec
sorting block 1, #reads = 1093039 mem = 536877752 peak = 787861504
[M::mem_process_seqs] Processed 715660 reads in 101.365 CPU sec, 3.276 real sec
sorting block 2, #reads = 1092393 mem = 536879569 peak = 900526080
[M::process] read 715812 sequences (100000215 bp) in 1.615 CPU sec, 1.683 real sec...
[M::process] read 715914 sequences (100000067 bp) in 1.110 CPU sec, 1.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304419, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (187.35, 74.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715762 reads in 85.648 CPU sec, 2.764 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304476, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (187.05, 73.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715852 sequences (100000111 bp) in 1.105 CPU sec, 1.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304099, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.69, 74.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715820 reads in 243.166 CPU sec, 7.816 real sec
sorting block 3, #reads = 1092322 mem = 536878758 peak = 900526080
[M::mem_process_seqs] Processed 715812 reads in 187.604 CPU sec, 6.090 real sec
sorting block 4, #reads = 1092828 mem = 536878789 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304372, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (187.06, 73.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715714 sequences (100000064 bp) in 1.905 CPU sec, 2.081 real sec...
[M::process] read 716226 sequences (100000220 bp) in 1.020 CPU sec, 1.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304397, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.39, 70.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715914 reads in 159.148 CPU sec, 5.119 real sec
[M::mem_process_seqs] Processed 715852 reads in 101.936 CPU sec, 3.317 real sec
sorting block 5, #reads = 1092859 mem = 536878209 peak = 900526080
[M::process] read 715892 sequences (100000078 bp) in 1.455 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303947, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (184.10, 70.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 465)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716136 sequences (100000165 bp) in 1.120 CPU sec, 1.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304722, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (179.25, 64.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304867, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 352)
[M::mem_pestat] mean and std.dev: (177.26, 62.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715714 reads in 231.021 CPU sec, 7.463 real sec
[M::mem_process_seqs] Processed 715892 reads in 173.509 CPU sec, 5.665 real sec
sorting block 6, #reads = 1092850 mem = 536877719 peak = 900526080
[M::mem_process_seqs] Processed 716226 reads in 189.082 CPU sec, 6.222 real sec
[M::process] read 715694 sequences (100000264 bp) in 1.909 CPU sec, 2.090 real sec...
sorting block 7, #reads = 1092528 mem = 536880230 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304458, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 355)
[M::mem_pestat] mean and std.dev: (178.21, 63.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 431)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715846 sequences (100000199 bp) in 1.732 CPU sec, 1.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304560, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.95, 71.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715344 sequences (100000134 bp) in 1.120 CPU sec, 1.122 real sec...
[M::mem_process_seqs] Processed 716136 reads in 168.911 CPU sec, 5.427 real sec
[M::mem_process_seqs] Processed 715694 reads in 106.628 CPU sec, 3.429 real sec
sorting block 8, #reads = 1093253 mem = 536877242 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304618, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.41, 70.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715694 sequences (100000167 bp) in 2.109 CPU sec, 2.312 real sec...
[M::process] read 715770 sequences (100000213 bp) in 1.072 CPU sec, 1.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304482, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (188.41, 75.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715846 reads in 161.828 CPU sec, 5.199 real sec
sorting block 9, #reads = 1093103 mem = 536878602 peak = 900526080
[M::mem_process_seqs] Processed 715344 reads in 103.092 CPU sec, 3.343 real sec
sorting block 10, #reads = 1092684 mem = 536880783 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304083, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (186.10, 72.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715700 sequences (100000024 bp) in 1.794 CPU sec, 1.937 real sec...
[M::process] read 715606 sequences (100000161 bp) in 1.170 CPU sec, 1.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304537, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (183.16, 68.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 459)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715694 reads in 159.144 CPU sec, 5.108 real sec
[M::mem_process_seqs] Processed 715770 reads in 101.647 CPU sec, 3.297 real sec
sorting block 11, #reads = 1092183 mem = 536880090 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304410, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (186.33, 72.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715616 sequences (100000105 bp) in 1.730 CPU sec, 1.889 real sec...
[M::process] read 716086 sequences (100000103 bp) in 1.180 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304658, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (186.22, 72.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715700 reads in 162.563 CPU sec, 5.222 real sec
[M::mem_process_seqs] Processed 715606 reads in 103.116 CPU sec, 3.351 real sec
sorting block 12, #reads = 1092531 mem = 536881918 peak = 900526080
[M::process] read 716484 sequences (100000231 bp) in 1.555 CPU sec, 1.635 real sec...
[M::process] read 716160 sequences (100000083 bp) in 1.150 CPU sec, 1.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304596, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367)
[M::mem_pestat] mean and std.dev: (181.50, 67.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716086 reads in 87.378 CPU sec, 2.823 real sec
sorting block 13, #reads = 1092773 mem = 536876876 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304096, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (185.44, 71.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 156, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243)
[M::mem_pestat] mean and std.dev: (327.40, 328.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2986)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304676, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 352)
[M::mem_pestat] mean and std.dev: (177.46, 62.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716024 sequences (100000137 bp) in 1.287 CPU sec, 1.289 real sec...
[M::mem_process_seqs] Processed 715616 reads in 247.773 CPU sec, 7.996 real sec
[M::mem_process_seqs] Processed 716484 reads in 189.760 CPU sec, 6.170 real sec
sorting block 14, #reads = 1092229 mem = 536879553 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304387, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (182.05, 67.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 453)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715924 sequences (100000081 bp) in 2.222 CPU sec, 2.422 real sec...
[M::process] read 715750 sequences (100000022 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304225, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.43, 70.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716160 reads in 158.369 CPU sec, 5.073 real sec
sorting block 15, #reads = 1092849 mem = 536878916 peak = 900526080
[M::mem_process_seqs] Processed 716024 reads in 101.619 CPU sec, 3.286 real sec
sorting block 16, #reads = 1092670 mem = 536878558 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304499, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.27, 70.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715450 sequences (100000273 bp) in 2.186 CPU sec, 2.410 real sec...
[M::process] read 715812 sequences (100000150 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304064, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (185.77, 72.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715924 reads in 156.260 CPU sec, 4.998 real sec
[M::mem_process_seqs] Processed 715750 reads in 100.223 CPU sec, 3.229 real sec
sorting block 17, #reads = 1092982 mem = 536878245 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304324, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (189.12, 76.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715556 sequences (100000123 bp) in 2.155 CPU sec, 2.362 real sec...
[M::process] read 715574 sequences (100000148 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304155, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (187.84, 74.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715450 reads in 154.381 CPU sec, 4.940 real sec
[M::mem_process_seqs] Processed 715812 reads in 99.063 CPU sec, 3.184 real sec
sorting block 18, #reads = 1092313 mem = 536881469 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304343, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (190.92, 78.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715230 sequences (100000100 bp) in 2.027 CPU sec, 2.232 real sec...
[M::process] read 715360 sequences (100000286 bp) in 1.470 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304084, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (188.75, 76.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715556 reads in 155.182 CPU sec, 4.967 real sec
sorting block 19, #reads = 1092402 mem = 536878642 peak = 900526080
[M::mem_process_seqs] Processed 715574 reads in 100.070 CPU sec, 3.220 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304082, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (193.67, 82.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1092507 mem = 536879908 peak = 900526080
[M::process] read 715430 sequences (100000152 bp) in 2.075 CPU sec, 2.198 real sec...
[M::process] read 715254 sequences (100000203 bp) in 1.460 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304044, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (190.65, 78.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715230 reads in 156.166 CPU sec, 5.000 real sec
[M::mem_process_seqs] Processed 715360 reads in 100.471 CPU sec, 3.232 real sec
sorting block 21, #reads = 1092266 mem = 536879641 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304319, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (188.02, 74.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715162 sequences (100000171 bp) in 2.297 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304169, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (190.96, 78.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 47, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1905)
[M::mem_pestat] mean and std.dev: (146.25, 230.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2538)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 714878 sequences (100000258 bp) in 1.440 CPU sec, 1.443 real sec...
[M::mem_process_seqs] Processed 715430 reads in 156.759 CPU sec, 5.010 real sec
[M::mem_process_seqs] Processed 715254 reads in 100.701 CPU sec, 3.247 real sec
sorting block 22, #reads = 1092005 mem = 536878310 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304093, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (194.51, 83.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714762 sequences (100000013 bp) in 2.313 CPU sec, 2.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304041, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (198.12, 89.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714956 sequences (100000009 bp) in 1.470 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 715162 reads in 157.200 CPU sec, 5.030 real sec
sorting block 23, #reads = 1092630 mem = 536879521 peak = 900526080
[M::mem_process_seqs] Processed 714878 reads in 100.714 CPU sec, 3.241 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303893, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (202.22, 94.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1091857 mem = 536877808 peak = 900526080
[M::process] read 715346 sequences (100000277 bp) in 1.931 CPU sec, 2.068 real sec...
[M::process] read 715458 sequences (100000022 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303787, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (202.76, 95.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714762 reads in 157.293 CPU sec, 5.038 real sec
[M::mem_process_seqs] Processed 714956 reads in 103.289 CPU sec, 3.328 real sec
sorting block 25, #reads = 1091498 mem = 536879236 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303985, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (194.73, 84.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715328 sequences (100000169 bp) in 2.382 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304019, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (189.83, 77.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715530 sequences (100000105 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_process_seqs] Processed 715346 reads in 156.185 CPU sec, 4.987 real sec
[M::mem_process_seqs] Processed 715458 reads in 100.537 CPU sec, 3.227 real sec
sorting block 26, #reads = 1091969 mem = 536877801 peak = 900526080
[M::process] read 715400 sequences (100000027 bp) in 2.034 CPU sec, 2.181 real sec...
[M::process] read 716322 sequences (100000168 bp) in 1.470 CPU sec, 1.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304194, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (189.29, 76.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715530 reads in 84.706 CPU sec, 2.697 real sec
sorting block 27, #reads = 1092360 mem = 536878463 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304285, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (192.08, 80.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304075, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (187.54, 74.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715928 sequences (100000001 bp) in 1.431 CPU sec, 1.439 real sec...
[M::mem_process_seqs] Processed 715328 reads in 238.846 CPU sec, 7.641 real sec
[M::mem_process_seqs] Processed 715400 reads in 114.535 CPU sec, 3.731 real sec
sorting block 28, #reads = 1092215 mem = 536880333 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304529, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 351)
[M::mem_pestat] mean and std.dev: (177.00, 62.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 93, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368)
[M::mem_pestat] mean and std.dev: (224.20, 229.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1802)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715902 sequences (100000221 bp) in 2.690 CPU sec, 3.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304197, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (183.35, 69.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716322 reads in 154.054 CPU sec, 4.929 real sec
[M::mem_process_seqs] Processed 715928 reads in 99.424 CPU sec, 3.178 real sec
[M::process] read 715534 sequences (100000097 bp) in 1.714 CPU sec, 1.750 real sec...
sorting block 29, #reads = 1091903 mem = 536879404 peak = 900526080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 305385, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (186.81, 73.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715586 sequences (100000216 bp) in 2.002 CPU sec, 2.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303928, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (188.87, 75.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715564 sequences (100000128 bp) in 1.420 CPU sec, 1.428 real sec...
[M::mem_process_seqs] Processed 715902 reads in 151.913 CPU sec, 4.868 real sec
sorting block 30, #reads = 1092503 mem = 536881476 peak = 900526080
[M::mem_process_seqs] Processed 715534 reads in 98.236 CPU sec, 3.159 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304520, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (188.89, 76.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1092565 mem = 536877943 peak = 924282880
[M::process] read 715458 sequences (100000115 bp) in 2.486 CPU sec, 2.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304237, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (188.59, 75.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715560 sequences (100000046 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 715586 reads in 155.915 CPU sec, 4.991 real sec
[M::mem_process_seqs] Processed 715564 reads in 101.756 CPU sec, 3.278 real sec
sorting block 32, #reads = 1092303 mem = 536880750 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304492, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.23, 77.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715388 sequences (100000061 bp) in 2.185 CPU sec, 2.313 real sec...
[M::process] read 715350 sequences (100000032 bp) in 1.500 CPU sec, 1.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304053, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (190.94, 79.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715458 reads in 154.684 CPU sec, 4.941 real sec
[M::mem_process_seqs] Processed 715560 reads in 99.942 CPU sec, 3.222 real sec
sorting block 33, #reads = 1092428 mem = 536878459 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303685, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (191.72, 80.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 14, 135)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393)
[M::mem_pestat] mean and std.dev: (37.09, 49.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 522)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715286 sequences (100000274 bp) in 2.135 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304416, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (194.97, 84.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715218 sequences (100000122 bp) in 1.560 CPU sec, 1.560 real sec...
[M::mem_process_seqs] Processed 715388 reads in 156.397 CPU sec, 5.005 real sec
sorting block 34, #reads = 1092189 mem = 536878924 peak = 924282880
[M::mem_process_seqs] Processed 715350 reads in 101.179 CPU sec, 3.252 real sec
sorting block 35, #reads = 1092013 mem = 536880049 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304013, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (196.20, 86.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 53, 1566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4648)
[M::mem_pestat] mean and std.dev: (538.67, 654.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6189)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715012 sequences (100000198 bp) in 2.611 CPU sec, 2.850 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304232, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (196.08, 86.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715200 sequences (100000283 bp) in 1.480 CPU sec, 1.476 real sec...
[M::mem_process_seqs] Processed 715286 reads in 154.899 CPU sec, 4.944 real sec
[M::process] read 714910 sequences (100000215 bp) in 1.451 CPU sec, 1.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304119, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (198.22, 89.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715012 reads in 85.974 CPU sec, 2.760 real sec
sorting block 36, #reads = 1091958 mem = 536879261 peak = 924282880
[M::mem_process_seqs] Processed 715218 reads in 208.942 CPU sec, 6.714 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303816, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (198.63, 90.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1092228 mem = 536879702 peak = 924282880
[M::process] read 714672 sequences (100000047 bp) in 2.626 CPU sec, 2.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303614, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (201.43, 93.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715200 reads in 175.709 CPU sec, 5.630 real sec
[M::process] read 714546 sequences (100000249 bp) in 1.646 CPU sec, 1.657 real sec...
[M::mem_process_seqs] Processed 714910 reads in 104.052 CPU sec, 3.327 real sec
sorting block 38, #reads = 1092054 mem = 536878558 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303341, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (204.93, 98.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714508 sequences (100000171 bp) in 2.415 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303537, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (206.77, 100.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715264 sequences (100000005 bp) in 1.460 CPU sec, 1.467 real sec...
[M::mem_process_seqs] Processed 714672 reads in 157.250 CPU sec, 5.026 real sec
[M::mem_process_seqs] Processed 714546 reads in 100.172 CPU sec, 3.212 real sec
sorting block 39, #reads = 1091526 mem = 536878223 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303443, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (205.97, 99.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715332 sequences (100000041 bp) in 2.486 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303929, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (196.78, 87.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715330 sequences (100000216 bp) in 1.610 CPU sec, 1.609 real sec...
[M::mem_process_seqs] Processed 714508 reads in 156.804 CPU sec, 5.006 real sec
sorting block 40, #reads = 1091599 mem = 536879002 peak = 924282880
[M::mem_process_seqs] Processed 715264 reads in 101.297 CPU sec, 3.246 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303952, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (196.89, 87.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1091781 mem = 536878893 peak = 924282880
[M::process] read 715446 sequences (100000212 bp) in 1.993 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303957, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (189.59, 76.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715812 sequences (100000208 bp) in 1.600 CPU sec, 1.600 real sec...
[M::mem_process_seqs] Processed 715332 reads in 154.482 CPU sec, 4.943 real sec
[M::mem_process_seqs] Processed 715330 reads in 99.898 CPU sec, 3.210 real sec
sorting block 42, #reads = 1092391 mem = 536878725 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303897, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.25, 77.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716274 sequences (100000023 bp) in 3.019 CPU sec, 3.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304473, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (187.53, 74.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715446 reads in 156.001 CPU sec, 4.985 real sec
[M::mem_process_seqs] Processed 715812 reads in 101.928 CPU sec, 3.256 real sec
[M::process] read 716016 sequences (100000238 bp) in 1.976 CPU sec, 2.037 real sec...
sorting block 43, #reads = 1091679 mem = 536880067 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304754, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (183.65, 69.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715922 sequences (100000101 bp) in 2.074 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304485, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (187.15, 74.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715570 sequences (100000036 bp) in 1.450 CPU sec, 1.458 real sec...
[M::mem_process_seqs] Processed 716274 reads in 154.348 CPU sec, 4.941 real sec
sorting block 44, #reads = 1092660 mem = 536878586 peak = 924282880
[M::mem_process_seqs] Processed 716016 reads in 100.765 CPU sec, 3.233 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304602, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (188.77, 76.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1092708 mem = 536882027 peak = 924282880
[M::process] read 715416 sequences (100000050 bp) in 2.932 CPU sec, 3.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 304352, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (188.90, 76.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 423, 9591)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28267)
[M::mem_pestat] mean and std.dev: (3607.30, 4228.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 37605)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715922 reads in 154.584 CPU sec, 4.937 real sec
[M::mem_process_seqs] Processed 715570 reads in 101.306 CPU sec, 3.239 real sec
[M::process] read 715578 sequences (100000077 bp) in 1.988 CPU sec, 2.069 real sec...
sorting block 46, #reads = 1092613 mem = 536882790 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303945, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (190.47, 78.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715358 sequences (100000015 bp) in 2.242 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304083, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (191.55, 79.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715416 reads in 154.539 CPU sec, 4.946 real sec
[M::process] read 715392 sequences (100000115 bp) in 1.520 CPU sec, 1.524 real sec...
[M::mem_process_seqs] Processed 715578 reads in 101.236 CPU sec, 3.243 real sec
sorting block 47, #reads = 1092247 mem = 536879137 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304441, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (192.71, 81.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715266 sequences (100000271 bp) in 2.644 CPU sec, 2.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304102, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (193.15, 82.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 694, 2601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7715)
[M::mem_pestat] mean and std.dev: (1358.10, 1506.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10272)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715204 sequences (100000178 bp) in 1.410 CPU sec, 1.414 real sec...
[M::mem_process_seqs] Processed 715358 reads in 153.565 CPU sec, 4.898 real sec
[M::mem_process_seqs] Processed 715392 reads in 98.413 CPU sec, 3.157 real sec
sorting block 48, #reads = 1092289 mem = 536877009 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304304, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (192.74, 82.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1091827 mem = 536877810 peak = 924282880
[M::process] read 715198 sequences (100000203 bp) in 2.259 CPU sec, 2.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304151, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (194.69, 84.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715196 sequences (100000207 bp) in 1.390 CPU sec, 1.395 real sec...
[M::mem_process_seqs] Processed 715266 reads in 146.204 CPU sec, 4.676 real sec
[M::mem_process_seqs] Processed 715204 reads in 90.235 CPU sec, 2.882 real sec
sorting block 50, #reads = 1092257 mem = 536878402 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304334, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (196.61, 87.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715220 sequences (100000245 bp) in 2.540 CPU sec, 2.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304398, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (198.71, 90.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714998 sequences (100000031 bp) in 1.460 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 715198 reads in 144.355 CPU sec, 4.594 real sec
[M::mem_process_seqs] Processed 715196 reads in 88.620 CPU sec, 2.827 real sec
sorting block 51, #reads = 1092032 mem = 536880319 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 303840, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (196.09, 86.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715006 sequences (100000075 bp) in 2.001 CPU sec, 2.125 real sec...
[M::process] read 714584 sequences (100000024 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303875, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (201.20, 93.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715220 reads in 145.461 CPU sec, 4.645 real sec
sorting block 52, #reads = 1092078 mem = 536879964 peak = 924282880
[M::process] read 714392 sequences (100000260 bp) in 1.590 CPU sec, 1.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304312, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (200.11, 92.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715006 reads in 81.534 CPU sec, 2.752 real sec
[M::mem_process_seqs] Processed 714998 reads in 195.351 CPU sec, 6.405 real sec
sorting block 53, #reads = 1092639 mem = 536879103 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303642, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (204.95, 98.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714678 sequences (100000007 bp) in 1.969 CPU sec, 2.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303507, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (205.84, 99.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 207, 8430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25002)
[M::mem_pestat] mean and std.dev: (2585.18, 3619.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33288)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715186 sequences (100000094 bp) in 1.560 CPU sec, 1.561 real sec...
[M::mem_process_seqs] Processed 714584 reads in 158.524 CPU sec, 5.060 real sec
[M::mem_process_seqs] Processed 714392 reads in 91.888 CPU sec, 2.933 real sec
sorting block 54, #reads = 1091493 mem = 536879726 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304112, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (201.25, 93.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715216 sequences (100000026 bp) in 2.543 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304286, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (194.50, 83.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714678 reads in 144.139 CPU sec, 4.606 real sec
sorting block 55, #reads = 1091494 mem = 536879917 peak = 924282880
[M::mem_process_seqs] Processed 715186 reads in 90.249 CPU sec, 2.874 real sec
[M::process] read 714986 sequences (100000086 bp) in 2.034 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303948, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.35, 88.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1091745 mem = 536881239 peak = 924282880
[M::process] read 715306 sequences (100000202 bp) in 2.391 CPU sec, 2.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303844, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (196.34, 86.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715216 reads in 142.443 CPU sec, 4.579 real sec
[M::mem_process_seqs] Processed 714986 reads in 92.405 CPU sec, 2.959 real sec
[M::process] read 717560 sequences (100000278 bp) in 2.788 CPU sec, 3.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304514, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (192.03, 80.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 57, #reads = 1091937 mem = 536880471 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 305477, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 303)
[M::mem_pestat] mean and std.dev: (160.44, 39.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715306 reads in 137.795 CPU sec, 4.449 real sec
[M::mem_process_seqs] Processed 717560 reads in 85.090 CPU sec, 2.734 real sec
[M::process] read 716442 sequences (100000171 bp) in 3.092 CPU sec, 3.431 real sec...
sorting block 58, #reads = 1091865 mem = 536880512 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304124, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 339)
[M::mem_pestat] mean and std.dev: (172.74, 56.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 403)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716442 reads in 72.228 CPU sec, 2.393 real sec
[M::process] read 716170 sequences (100000036 bp) in 2.856 CPU sec, 3.161 real sec...
sorting block 59, #reads = 1093151 mem = 536877827 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304106, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 163, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 357)
[M::mem_pestat] mean and std.dev: (178.54, 63.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716170 reads in 72.983 CPU sec, 2.380 real sec
[M::process] read 715976 sequences (100000183 bp) in 2.578 CPU sec, 2.777 real sec...
sorting block 60, #reads = 1092522 mem = 536878685 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304516, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 364)
[M::mem_pestat] mean and std.dev: (180.47, 65.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715976 reads in 74.149 CPU sec, 2.419 real sec
[M::process] read 715874 sequences (100000140 bp) in 3.154 CPU sec, 3.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304226, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (182.89, 68.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715818 sequences (100000061 bp) in 2.221 CPU sec, 2.342 real sec...
[M::mem_process_seqs] Processed 715874 reads in 74.939 CPU sec, 2.429 real sec
sorting block 61, #reads = 1092452 mem = 536878264 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303970, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (186.28, 72.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715762 sequences (100000105 bp) in 2.322 CPU sec, 2.464 real sec...
[M::mem_process_seqs] Processed 715818 reads in 75.241 CPU sec, 2.443 real sec
sorting block 62, #reads = 1092340 mem = 536879186 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304403, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.56, 70.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715762 reads in 75.270 CPU sec, 2.447 real sec
[M::process] read 715922 sequences (100000108 bp) in 2.528 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304314, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.76, 71.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715922 reads in 73.614 CPU sec, 2.400 real sec
sorting block 63, #reads = 1092406 mem = 536879762 peak = 924282880
[M::process] read 715838 sequences (100000243 bp) in 3.041 CPU sec, 3.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 304429, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367)
[M::mem_pestat] mean and std.dev: (181.07, 66.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 448)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715842 sequences (100000180 bp) in 2.164 CPU sec, 2.269 real sec...
[M::mem_process_seqs] Processed 715838 reads in 71.442 CPU sec, 2.320 real sec
sorting block 64, #reads = 1092457 mem = 536880235 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304395, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.30, 70.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715842 reads in 70.539 CPU sec, 2.289 real sec
[M::process] read 716034 sequences (100000170 bp) in 2.390 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304334, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (179.22, 64.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716034 reads in 72.486 CPU sec, 2.355 real sec
[M::process] read 716286 sequences (100000180 bp) in 2.477 CPU sec, 2.633 real sec...
sorting block 65, #reads = 1093082 mem = 536878942 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304679, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 339)
[M::mem_pestat] mean and std.dev: (172.98, 56.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 403)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716286 reads in 73.440 CPU sec, 2.393 real sec
[M::process] read 716162 sequences (100000084 bp) in 2.611 CPU sec, 2.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304702, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 342)
[M::mem_pestat] mean and std.dev: (174.23, 58.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 408)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716162 reads in 72.042 CPU sec, 2.345 real sec
sorting block 66, #reads = 1092763 mem = 536880812 peak = 924282880
[M::process] read 716058 sequences (100000035 bp) in 3.080 CPU sec, 3.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304685, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 349)
[M::mem_pestat] mean and std.dev: (176.43, 61.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715892 sequences (100000179 bp) in 2.150 CPU sec, 2.238 real sec...
[M::mem_process_seqs] Processed 716058 reads in 73.912 CPU sec, 2.398 real sec
sorting block 67, #reads = 1093021 mem = 536880879 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304366, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 346)
[M::mem_pestat] mean and std.dev: (175.66, 59.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 415)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 75, 175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (65.38, 67.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715892 reads in 73.996 CPU sec, 2.406 real sec
[M::process] read 715744 sequences (100000253 bp) in 2.477 CPU sec, 2.619 real sec...
sorting block 68, #reads = 1092829 mem = 536879058 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304455, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.59, 70.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715744 reads in 75.332 CPU sec, 2.448 real sec
[M::process] read 715654 sequences (100000088 bp) in 2.654 CPU sec, 2.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304442, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (185.84, 72.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 112, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (137.09, 125.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715808 sequences (100000168 bp) in 2.275 CPU sec, 2.397 real sec...
[M::mem_process_seqs] Processed 715654 reads in 74.346 CPU sec, 2.411 real sec
sorting block 69, #reads = 1092538 mem = 536881238 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304309, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (181.95, 67.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 453)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715808 reads in 74.912 CPU sec, 2.436 real sec
[M::process] read 716018 sequences (100000196 bp) in 2.626 CPU sec, 2.812 real sec...
sorting block 70, #reads = 1092443 mem = 536878849 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304633, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 163, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 351)
[M::mem_pestat] mean and std.dev: (176.98, 61.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715934 sequences (100000027 bp) in 2.188 CPU sec, 2.305 real sec...
[M::mem_process_seqs] Processed 716018 reads in 74.740 CPU sec, 2.427 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303924, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 349)
[M::mem_pestat] mean and std.dev: (176.28, 61.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 420)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716224 sequences (100000039 bp) in 2.351 CPU sec, 2.504 real sec...
[M::mem_process_seqs] Processed 715934 reads in 74.414 CPU sec, 2.413 real sec
sorting block 71, #reads = 1092932 mem = 536877475 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304596, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 351)
[M::mem_pestat] mean and std.dev: (176.65, 61.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716224 reads in 74.352 CPU sec, 2.414 real sec
[M::process] read 716048 sequences (100000141 bp) in 2.531 CPU sec, 2.696 real sec...
sorting block 72, #reads = 1092853 mem = 536880178 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304384, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 343)
[M::mem_pestat] mean and std.dev: (173.99, 58.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 408)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716048 reads in 73.992 CPU sec, 2.407 real sec
[M::process] read 716628 sequences (100000060 bp) in 2.611 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304111, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 321)
[M::mem_pestat] mean and std.dev: (166.47, 48.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 379)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716674 sequences (100000205 bp) in 2.196 CPU sec, 2.313 real sec...
[M::mem_process_seqs] Processed 716628 reads in 74.526 CPU sec, 2.428 real sec
sorting block 73, #reads = 1092571 mem = 536877944 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304876, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 345)
[M::mem_pestat] mean and std.dev: (174.67, 59.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 412)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716674 reads in 73.240 CPU sec, 2.382 real sec
[M::process] read 716364 sequences (100000119 bp) in 3.195 CPU sec, 3.528 real sec...
sorting block 74, #reads = 1093217 mem = 536880553 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304349, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 360)
[M::mem_pestat] mean and std.dev: (179.14, 64.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 438)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 112, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (141.25, 194.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 716116 sequences (100000258 bp) in 2.292 CPU sec, 2.428 real sec...
[M::mem_process_seqs] Processed 716364 reads in 75.350 CPU sec, 2.445 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304242, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (182.77, 68.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716086 sequences (100000014 bp) in 2.281 CPU sec, 2.412 real sec...
[M::mem_process_seqs] Processed 716116 reads in 75.106 CPU sec, 2.437 real sec
sorting block 75, #reads = 1092323 mem = 536879061 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304344, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (183.74, 69.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716086 reads in 73.541 CPU sec, 2.392 real sec
[M::process] read 715736 sequences (100000148 bp) in 2.357 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303697, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (185.51, 71.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1091424 mem = 536876957 peak = 924282880
[M::mem_process_seqs] Processed 715736 reads in 73.429 CPU sec, 2.396 real sec
[M::process] read 715820 sequences (100000125 bp) in 2.589 CPU sec, 2.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304126, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.57, 70.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715692 sequences (100000149 bp) in 2.155 CPU sec, 2.274 real sec...
[M::mem_process_seqs] Processed 715820 reads in 73.984 CPU sec, 2.399 real sec
sorting block 77, #reads = 1092003 mem = 536881668 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303974, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (186.83, 73.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715692 reads in 68.167 CPU sec, 2.219 real sec
[M::process] read 715762 sequences (100000139 bp) in 2.745 CPU sec, 2.955 real sec...
sorting block 78, #reads = 1091534 mem = 536881383 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304210, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (188.51, 75.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715348 sequences (100000097 bp) in 2.007 CPU sec, 2.075 real sec...
[M::mem_process_seqs] Processed 715762 reads in 72.839 CPU sec, 2.359 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303911, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.44, 74.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715588 sequences (100000234 bp) in 2.275 CPU sec, 2.406 real sec...
[M::mem_process_seqs] Processed 715348 reads in 73.391 CPU sec, 2.377 real sec
sorting block 79, #reads = 1092633 mem = 536882753 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304176, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (189.21, 76.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715544 sequences (100000231 bp) in 2.288 CPU sec, 2.452 real sec...
[M::mem_process_seqs] Processed 715588 reads in 72.712 CPU sec, 2.354 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304366, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (187.85, 74.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715636 sequences (100000182 bp) in 2.278 CPU sec, 2.424 real sec...
[M::mem_process_seqs] Processed 715544 reads in 72.817 CPU sec, 2.360 real sec
sorting block 80, #reads = 1091923 mem = 536879442 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304232, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (186.19, 72.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715636 reads in 72.768 CPU sec, 2.362 real sec
[M::process] read 715252 sequences (100000247 bp) in 2.670 CPU sec, 2.880 real sec...
sorting block 81, #reads = 1092557 mem = 536878674 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303844, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (188.07, 75.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715252 reads in 71.722 CPU sec, 2.329 real sec
[M::process] read 715126 sequences (100000130 bp) in 2.289 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303413, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (189.91, 77.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715126 reads in 73.232 CPU sec, 2.379 real sec
sorting block 82, #reads = 1091922 mem = 536882096 peak = 924282880
[M::process] read 715150 sequences (100000171 bp) in 2.561 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303870, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (192.90, 82.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715150 reads in 72.366 CPU sec, 2.353 real sec
[M::process] read 715266 sequences (100000255 bp) in 2.494 CPU sec, 2.732 real sec...
sorting block 83, #reads = 1091549 mem = 536879622 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303918, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (196.78, 87.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715266 reads in 72.797 CPU sec, 2.377 real sec
[M::process] read 715004 sequences (100000089 bp) in 3.283 CPU sec, 3.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303508, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.35, 88.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715620 sequences (100000195 bp) in 2.186 CPU sec, 2.317 real sec...
[M::mem_process_seqs] Processed 715004 reads in 73.902 CPU sec, 2.398 real sec
sorting block 84, #reads = 1091293 mem = 536878780 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304187, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (185.12, 71.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715460 sequences (100000095 bp) in 2.298 CPU sec, 2.435 real sec...
[M::mem_process_seqs] Processed 715620 reads in 73.234 CPU sec, 2.372 real sec
sorting block 85, #reads = 1091891 mem = 536879391 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304125, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (185.85, 72.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715460 reads in 72.751 CPU sec, 2.359 real sec
[M::process] read 716120 sequences (100000093 bp) in 2.280 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304411, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (178.67, 64.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 435)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716120 reads in 73.027 CPU sec, 2.372 real sec
sorting block 86, #reads = 1092402 mem = 536879033 peak = 924282880
[M::process] read 715954 sequences (100000123 bp) in 2.737 CPU sec, 2.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304652, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 215)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 349)
[M::mem_pestat] mean and std.dev: (175.88, 60.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716224 sequences (100000009 bp) in 2.071 CPU sec, 2.171 real sec...
[M::mem_process_seqs] Processed 715954 reads in 72.328 CPU sec, 2.340 real sec
sorting block 87, #reads = 1092188 mem = 536877617 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304470, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 342)
[M::mem_pestat] mean and std.dev: (173.33, 57.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 407)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716542 sequences (100000266 bp) in 2.296 CPU sec, 2.428 real sec...
[M::mem_process_seqs] Processed 716224 reads in 72.685 CPU sec, 2.358 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304392, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 348)
[M::mem_pestat] mean and std.dev: (175.66, 60.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716136 sequences (100000200 bp) in 2.259 CPU sec, 2.419 real sec...
[M::mem_process_seqs] Processed 716542 reads in 73.823 CPU sec, 2.397 real sec
sorting block 88, #reads = 1092994 mem = 536878363 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303896, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (185.93, 72.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716136 reads in 72.883 CPU sec, 2.367 real sec
[M::process] read 715958 sequences (100000160 bp) in 2.334 CPU sec, 2.484 real sec...
sorting block 89, #reads = 1093207 mem = 536878467 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304092, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (188.20, 75.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715958 reads in 72.774 CPU sec, 2.363 real sec
[M::process] read 715852 sequences (100000030 bp) in 2.356 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304671, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (187.89, 74.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715852 reads in 72.369 CPU sec, 2.356 real sec
sorting block 90, #reads = 1092757 mem = 536878371 peak = 924282880
[M::process] read 715576 sequences (100000276 bp) in 2.852 CPU sec, 3.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304218, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (188.66, 75.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715508 sequences (100000248 bp) in 2.028 CPU sec, 2.134 real sec...
[M::mem_process_seqs] Processed 715576 reads in 71.823 CPU sec, 2.324 real sec
sorting block 91, #reads = 1092304 mem = 536879737 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303819, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.31, 78.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715508 reads in 72.481 CPU sec, 2.348 real sec
[M::process] read 715538 sequences (100000144 bp) in 2.440 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303827, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (191.02, 79.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 43, 168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (46.75, 53.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715538 reads in 66.631 CPU sec, 2.165 real sec
[M::process] read 715364 sequences (100000146 bp) in 2.327 CPU sec, 2.515 real sec...
sorting block 92, #reads = 1091844 mem = 536878991 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303768, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (194.19, 83.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715364 reads in 71.919 CPU sec, 2.335 real sec
[M::process] read 715286 sequences (100000196 bp) in 2.460 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303762, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (190.79, 78.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 93, #reads = 1091089 mem = 536878355 peak = 924282880
[M::mem_process_seqs] Processed 715286 reads in 72.802 CPU sec, 2.367 real sec
[M::process] read 715428 sequences (100000269 bp) in 2.971 CPU sec, 3.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303778, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (193.59, 82.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715428 reads in 68.830 CPU sec, 2.235 real sec
[M::process] read 715316 sequences (100000196 bp) in 2.312 CPU sec, 2.604 real sec...
sorting block 94, #reads = 1091952 mem = 536878846 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303556, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.28, 85.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715316 reads in 70.708 CPU sec, 2.306 real sec
[M::process] read 715062 sequences (100000200 bp) in 2.967 CPU sec, 3.215 real sec...
sorting block 95, #reads = 1091337 mem = 536880094 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303268, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.21, 85.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715164 sequences (100000170 bp) in 1.839 CPU sec, 2.042 real sec...
[M::mem_process_seqs] Processed 715062 reads in 73.034 CPU sec, 2.359 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304160, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (194.74, 84.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714948 sequences (100000053 bp) in 2.444 CPU sec, 2.588 real sec...
[M::mem_process_seqs] Processed 715164 reads in 73.688 CPU sec, 2.385 real sec
sorting block 96, #reads = 1091033 mem = 536876823 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303578, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.08, 88.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714972 sequences (100000213 bp) in 2.218 CPU sec, 2.370 real sec...
[M::mem_process_seqs] Processed 714948 reads in 73.378 CPU sec, 2.376 real sec
sorting block 97, #reads = 1092248 mem = 536880591 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304073, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.19, 88.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715044 sequences (100000198 bp) in 2.262 CPU sec, 2.397 real sec...
[M::mem_process_seqs] Processed 714972 reads in 73.264 CPU sec, 2.378 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303659, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (199.84, 92.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715044 reads in 72.061 CPU sec, 2.337 real sec
[M::process] read 714696 sequences (100000035 bp) in 2.297 CPU sec, 2.479 real sec...
sorting block 98, #reads = 1091727 mem = 536879882 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303537, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517)
[M::mem_pestat] mean and std.dev: (199.03, 90.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714696 reads in 72.518 CPU sec, 2.356 real sec
[M::process] read 715274 sequences (100000278 bp) in 2.322 CPU sec, 2.470 real sec...
sorting block 99, #reads = 1091550 mem = 536876588 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303762, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (196.97, 88.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715274 reads in 72.805 CPU sec, 2.363 real sec
[M::process] read 715688 sequences (100000025 bp) in 2.236 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304532, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (186.20, 72.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715688 reads in 72.583 CPU sec, 2.364 real sec
[M::process] read 715676 sequences (100000131 bp) in 2.242 CPU sec, 2.372 real sec...
sorting block 100, #reads = 1092563 mem = 536877870 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304138, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (183.25, 69.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715676 reads in 71.244 CPU sec, 2.326 real sec
[M::process] read 715840 sequences (100000080 bp) in 2.747 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304519, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (181.13, 66.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715840 reads in 76.177 CPU sec, 2.493 real sec
sorting block 101, #reads = 1091330 mem = 536878105 peak = 924282880
[M::process] read 716006 sequences (100000095 bp) in 3.186 CPU sec, 3.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304748, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 352)
[M::mem_pestat] mean and std.dev: (176.54, 61.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716006 reads in 80.160 CPU sec, 2.621 real sec
[M::process] read 716696 sequences (100000045 bp) in 3.028 CPU sec, 3.370 real sec...
sorting block 102, #reads = 1092247 mem = 536878463 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 305054, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 716312 sequences (100000076 bp) in 1.979 CPU sec, 2.110 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 327)
[M::mem_pestat] mean and std.dev: (168.41, 51.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 387)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716138 sequences (100000093 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304074, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367)
[M::mem_pestat] mean and std.dev: (180.61, 66.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716696 reads in 142.277 CPU sec, 4.535 real sec
[M::mem_process_seqs] Processed 716312 reads in 89.518 CPU sec, 2.848 real sec
[M::process] read 715838 sequences (100000150 bp) in 1.649 CPU sec, 1.672 real sec...
sorting block 103, #reads = 1093088 mem = 536878948 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304379, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (184.72, 71.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715980 sequences (100000009 bp) in 2.613 CPU sec, 2.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304660, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.26, 74.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716138 reads in 140.673 CPU sec, 4.510 real sec
[M::mem_process_seqs] Processed 715838 reads in 90.948 CPU sec, 2.909 real sec
sorting block 104, #reads = 1092785 mem = 536880174 peak = 924282880
[M::process] read 715774 sequences (100000018 bp) in 1.877 CPU sec, 1.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304003, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.36, 74.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 105, #reads = 1092563 mem = 536879454 peak = 924282880
[M::process] read 715730 sequences (100000241 bp) in 2.003 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303798, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (188.13, 75.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715980 reads in 136.764 CPU sec, 4.355 real sec
[M::process] read 715622 sequences (100000109 bp) in 1.482 CPU sec, 1.488 real sec...
[M::mem_process_seqs] Processed 715774 reads in 81.992 CPU sec, 2.614 real sec
sorting block 106, #reads = 1092423 mem = 536877818 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304196, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.07, 74.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715490 sequences (100000181 bp) in 2.201 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304186, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (191.43, 79.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715730 reads in 136.495 CPU sec, 4.355 real sec
[M::mem_process_seqs] Processed 715622 reads in 82.122 CPU sec, 2.603 real sec
[M::process] read 715500 sequences (100000222 bp) in 1.660 CPU sec, 1.697 real sec...
sorting block 107, #reads = 1092437 mem = 536881728 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304207, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.23, 77.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715216 sequences (100000257 bp) in 2.058 CPU sec, 2.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303615, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (192.66, 81.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715490 reads in 133.218 CPU sec, 4.265 real sec
[M::mem_process_seqs] Processed 715500 reads in 77.558 CPU sec, 2.464 real sec
[M::process] read 715300 sequences (100000082 bp) in 1.796 CPU sec, 1.861 real sec...
sorting block 108, #reads = 1092121 mem = 536878424 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303860, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (193.15, 82.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715102 sequences (100000072 bp) in 2.180 CPU sec, 2.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303438, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (194.98, 85.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715216 reads in 140.117 CPU sec, 4.482 real sec
sorting block 109, #reads = 1091205 mem = 536878978 peak = 924282880
[M::mem_process_seqs] Processed 715300 reads in 85.800 CPU sec, 2.730 real sec
[M::process] read 714992 sequences (100000237 bp) in 1.916 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303734, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (198.14, 89.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 110, #reads = 1091916 mem = 536879288 peak = 924282880
[M::process] read 714820 sequences (100000152 bp) in 2.200 CPU sec, 2.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303855, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (198.17, 89.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715102 reads in 140.198 CPU sec, 4.484 real sec
[M::mem_process_seqs] Processed 714992 reads in 87.721 CPU sec, 2.798 real sec
[M::process] read 714788 sequences (100000208 bp) in 1.915 CPU sec, 2.003 real sec...
sorting block 111, #reads = 1091973 mem = 536877922 peak = 924282880
[M::process] read 714660 sequences (100000217 bp) in 2.429 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303385, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (200.19, 92.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714788 reads in 76.789 CPU sec, 2.433 real sec
sorting block 112, #reads = 1091640 mem = 536879121 peak = 924282880
[M::process] read 715038 sequences (100000108 bp) in 2.012 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303760, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (200.35, 92.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 27, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1630)
[M::mem_pestat] mean and std.dev: (428.09, 558.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2661)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303261, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (202.00, 94.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714820 reads in 216.748 CPU sec, 6.921 real sec
[M::mem_process_seqs] Processed 714660 reads in 98.852 CPU sec, 3.172 real sec
[M::process] read 715418 sequences (100000049 bp) in 1.981 CPU sec, 2.094 real sec...
sorting block 113, #reads = 1091684 mem = 536879010 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303354, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (201.96, 95.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715860 sequences (100000156 bp) in 2.392 CPU sec, 2.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304407, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (187.23, 73.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715038 reads in 136.304 CPU sec, 4.366 real sec
[M::mem_process_seqs] Processed 715418 reads in 79.752 CPU sec, 2.535 real sec
sorting block 114, #reads = 1091298 mem = 536878598 peak = 924282880
[M::process] read 715612 sequences (100000269 bp) in 2.738 CPU sec, 2.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304463, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (186.74, 73.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715860 reads in 74.671 CPU sec, 2.507 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304123, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (186.90, 73.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1092044 mem = 536877936 peak = 924282880
[M::mem_process_seqs] Processed 715612 reads in 73.805 CPU sec, 2.461 real sec
[M::process] read 715760 sequences (100000147 bp) in 2.772 CPU sec, 3.040 real sec...
[M::process] read 141384 sequences (19752064 bp) in 0.776 CPU sec, 0.870 real sec...
sorting block 116, #reads = 1092600 mem = 536877100 peak = 924282880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 60044, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (185.35, 72.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 141384 reads in 13.641 CPU sec, 0.451 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304321, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (186.07, 72.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715760 reads in 85.417 CPU sec, 2.996 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 128234580
overall: 69540229120 mem 14594.106 user 1611.545 sys 560.198 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.D0001483_22q.L003\tPU:L003\tSM:D0001483_22q\tLB:D0001483_22q\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.D0001483_22q_R1.fastq.gz 0003.D0001483_22q_R2.fastq.gz
[main] Real time: 560.239 sec; CPU: 16205.654 sec
sorting block 117, #reads = 1090694 mem = 536183963 peak = 924282880
uniq header count 1
merging 118 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 5388395851
output reads: 128886226
bam_mem_sort: 118 calls 42.455 user 1.045 sys 43.793 real
bam_write: 118 calls 77.644 user 2.898 sys 81.684 real
execute: 1 calls 26.995 user 1.681 sys 594.923 real
merge_files: 1 calls 26.976 user 1.651 sys 33.045 real
parse_chunk: 1888 calls 65.208 user 15.889 sys 82.961 real
read_chunk: 5108 calls 63.327 user 49.548 sys 502.274 real
sort_block: 1 calls 51.278 user 48.992 sys 483.259 real
write_chunk: 3218 calls 26.530 user 1.362 sys 32.141 real
overall: 2375909376 mem 1161.436 user 86.132 sys 594.945 real
ls: cannot access '*.cram': No such file or directory