Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e1/aaa50b8767713e1d2aceb45a3c4b20/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/42/3263d93f2f544374179b89a82bdfb2/SH032_HG514/0001.SH032_HG514_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/42/3263d93f2f544374179b89a82bdfb2/SH032_HG514/0001.SH032_HG514_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e1/aaa50b8767713e1d2aceb45a3c4b20/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o SH032_HG514.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1863.775 CPU sec, 70.191 real sec
[M::process] read 729486 sequences (100000244 bp) in 1.268 CPU sec, 1.272 real sec...
[M::process] read 727488 sequences (100000256 bp) in 1.733 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 307681, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 201, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (224.64, 109.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 248, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (272.55, 140.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 215, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (207.53, 80.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727486 sequences (100000062 bp) in 1.919 CPU sec, 1.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306794, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 208, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471)
[M::mem_pestat] mean and std.dev: (191.15, 65.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.13, 142.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (101, 147, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (170.55, 114.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727084 sequences (100000029 bp) in 1.874 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 729486 reads in 182.147 CPU sec, 5.922 real sec
[M::mem_process_seqs] Processed 727488 reads in 104.133 CPU sec, 3.418 real sec
sorting block 0, #reads = 1105144 mem = 536878475 peak = 602959872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306345, 9, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 234, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (212.05, 71.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (279.89, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 169, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (188.27, 70.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726298 sequences (100000042 bp) in 2.466 CPU sec, 2.662 real sec...
[M::process] read 726452 sequences (100000231 bp) in 1.633 CPU sec, 1.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307080, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 190, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438)
[M::mem_pestat] mean and std.dev: (195.00, 80.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 534)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (280.55, 143.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 230, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (224.62, 65.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727486 reads in 175.838 CPU sec, 5.610 real sec
[M::mem_process_seqs] Processed 727084 reads in 100.781 CPU sec, 3.226 real sec
sorting block 1, #reads = 1104121 mem = 536877801 peak = 764964864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306372, 9, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 197, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417)
[M::mem_pestat] mean and std.dev: (182.81, 61.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.57, 143.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 241, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (227.37, 92.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726894 sequences (100000050 bp) in 2.391 CPU sec, 2.537 real sec...
[M::process] read 727346 sequences (100000264 bp) in 1.777 CPU sec, 1.804 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306574, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 221, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (239.65, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.96, 144.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 145, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (175.12, 64.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726298 reads in 180.292 CPU sec, 5.754 real sec
[M::mem_process_seqs] Processed 726452 reads in 105.801 CPU sec, 3.422 real sec
sorting block 2, #reads = 1103963 mem = 536878799 peak = 816414720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306771, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 249, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 418)
[M::mem_pestat] mean and std.dev: (207.11, 56.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (281.67, 144.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 207, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (225.53, 87.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727930 sequences (100000220 bp) in 2.661 CPU sec, 2.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306955, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 138, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320)
[M::mem_pestat] mean and std.dev: (134.67, 45.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 392)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.57, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 172, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (216.55, 100.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728176 sequences (100000143 bp) in 1.838 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 726894 reads in 185.591 CPU sec, 5.989 real sec
sorting block 3, #reads = 1103693 mem = 536877336 peak = 816414720
[M::mem_process_seqs] Processed 727346 reads in 108.129 CPU sec, 3.509 real sec
sorting block 4, #reads = 1104238 mem = 536878404 peak = 911216640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 307096, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 193, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 329)
[M::mem_pestat] mean and std.dev: (185.25, 45.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 388)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.25, 142.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 199, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (182.53, 57.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 574)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727622 sequences (100000022 bp) in 2.433 CPU sec, 2.558 real sec...
[M::process] read 727056 sequences (100000122 bp) in 1.908 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307145, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 211, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (185.00, 73.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 575)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (277.18, 143.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 191, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (215.85, 99.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 659)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727930 reads in 185.158 CPU sec, 5.935 real sec
[M::mem_process_seqs] Processed 728176 reads in 104.206 CPU sec, 3.318 real sec
sorting block 5, #reads = 1104481 mem = 536877941 peak = 911216640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 307151, 2, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 142, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495)
[M::mem_pestat] mean and std.dev: (202.60, 109.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.76, 143.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 250, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 438)
[M::mem_pestat] mean and std.dev: (231.35, 60.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726774 sequences (100000038 bp) in 2.393 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306448, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 212, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (211.16, 71.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 727372 sequences (100000020 bp) in 1.820 CPU sec, 1.821 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.70, 143.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 158, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (195.82, 86.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727622 reads in 170.256 CPU sec, 5.441 real sec
[M::mem_process_seqs] Processed 727056 reads in 95.676 CPU sec, 3.045 real sec
sorting block 6, #reads = 1104853 mem = 536880021 peak = 911216640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306755, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 148, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362)
[M::mem_pestat] mean and std.dev: (152.47, 39.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (281.13, 144.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 199, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (220.00, 85.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727428 sequences (100000149 bp) in 2.152 CPU sec, 2.242 real sec...
[M::process] read 727546 sequences (100000221 bp) in 1.839 CPU sec, 1.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306892, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 163, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 346)
[M::mem_pestat] mean and std.dev: (178.80, 49.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.78, 143.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 269, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1708)
[M::mem_pestat] mean and std.dev: (273.23, 207.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2227)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726774 reads in 168.625 CPU sec, 5.401 real sec
sorting block 7, #reads = 1104155 mem = 536876911 peak = 911216640
[M::mem_process_seqs] Processed 727372 reads in 94.780 CPU sec, 3.035 real sec
sorting block 8, #reads = 1103891 mem = 536880337 peak = 915623936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306531, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 148, 165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 241)
[M::mem_pestat] mean and std.dev: (149.77, 38.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 305)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 254, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (278.82, 143.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 166, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390)
[M::mem_pestat] mean and std.dev: (177.55, 55.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 477)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727380 sequences (100000268 bp) in 1.867 CPU sec, 1.996 real sec...
[M::process] read 728544 sequences (100000045 bp) in 1.580 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306733, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (206.95, 63.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 535)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (280.40, 144.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 192, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 335)
[M::mem_pestat] mean and std.dev: (196.78, 56.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727428 reads in 162.099 CPU sec, 5.235 real sec
[M::mem_process_seqs] Processed 727546 reads in 96.339 CPU sec, 3.172 real sec
sorting block 9, #reads = 1104324 mem = 536879784 peak = 915623936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 306957, 4, 27)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.74, 143.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 184, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (200.74, 90.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 591)
[M::mem_pestat] skip orientation RR
[M::process] read 727340 sequences (100000148 bp) in 2.186 CPU sec, 2.279 real sec...
[M::process] read 727216 sequences (100000193 bp) in 1.772 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 307180, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 181, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404)
[M::mem_pestat] mean and std.dev: (180.19, 44.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (275.80, 142.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 176, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (170.33, 62.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727380 reads in 166.342 CPU sec, 5.307 real sec
[M::mem_process_seqs] Processed 728544 reads in 95.465 CPU sec, 3.038 real sec
sorting block 10, #reads = 1104459 mem = 536881576 peak = 915623936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306523, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 152, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 297)
[M::mem_pestat] mean and std.dev: (139.55, 29.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 356)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.25, 143.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 229, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (202.83, 66.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726846 sequences (100000223 bp) in 2.141 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306334, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 223, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 372)
[M::mem_pestat] mean and std.dev: (201.33, 43.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 726460 sequences (100000160 bp) in 1.857 CPU sec, 1.862 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.73, 144.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 168, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (185.76, 63.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727340 reads in 160.880 CPU sec, 5.112 real sec
sorting block 11, #reads = 1104966 mem = 536879702 peak = 915623936
[M::mem_process_seqs] Processed 727216 reads in 91.941 CPU sec, 2.911 real sec
sorting block 12, #reads = 1103733 mem = 536880381 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306695, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 192, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (201.88, 71.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.26, 144.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 198, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (209.35, 92.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726156 sequences (100000156 bp) in 2.278 CPU sec, 2.424 real sec...
[M::process] read 725872 sequences (100000244 bp) in 1.686 CPU sec, 1.692 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306352, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 219, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 466)
[M::mem_pestat] mean and std.dev: (236.14, 74.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.46, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 244, 1025)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2811)
[M::mem_pestat] mean and std.dev: (375.07, 404.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3704)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726846 reads in 167.461 CPU sec, 5.329 real sec
[M::mem_process_seqs] Processed 726460 reads in 98.493 CPU sec, 3.134 real sec
sorting block 13, #reads = 1103748 mem = 536878778 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306430, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 211, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (219.75, 106.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.24, 145.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 182, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 344)
[M::mem_pestat] mean and std.dev: (179.60, 55.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 412)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725978 sequences (100000164 bp) in 2.116 CPU sec, 2.259 real sec...
[M::process] read 726238 sequences (100000149 bp) in 1.751 CPU sec, 1.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306038, 9, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 199, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (185.54, 72.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (284.83, 146.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 207, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (205.38, 83.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726156 reads in 174.535 CPU sec, 5.569 real sec
[M::mem_process_seqs] Processed 725872 reads in 102.403 CPU sec, 3.257 real sec
sorting block 14, #reads = 1103284 mem = 536879150 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306421, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 151, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (203.38, 113.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.96, 146.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 219, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (203.44, 75.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726168 sequences (100000001 bp) in 2.667 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306407, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 222, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 415)
[M::mem_pestat] mean and std.dev: (204.20, 47.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.22, 145.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 235, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (220.85, 52.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 577)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725978 reads in 173.800 CPU sec, 5.518 real sec
[M::process] read 725486 sequences (100000182 bp) in 2.220 CPU sec, 2.228 real sec...
[M::mem_process_seqs] Processed 726238 reads in 100.518 CPU sec, 3.175 real sec
sorting block 15, #reads = 1103013 mem = 536879007 peak = 965054464
sorting block 16, #reads = 1103238 mem = 536879392 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306632, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 248, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (233.27, 81.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.70, 145.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 228, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (205.69, 77.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724896 sequences (100000079 bp) in 2.596 CPU sec, 2.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305958, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 255, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (216.42, 89.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 684)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.31, 145.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 200, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 314)
[M::mem_pestat] mean and std.dev: (180.38, 38.67)
[M::mem_pestat] low and high boundaries for proper pairs: (2, 366)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724366 sequences (100000160 bp) in 1.921 CPU sec, 1.925 real sec...
[M::mem_process_seqs] Processed 726168 reads in 173.820 CPU sec, 5.527 real sec
[M::mem_process_seqs] Processed 725486 reads in 100.049 CPU sec, 3.162 real sec
sorting block 17, #reads = 1103407 mem = 536879494 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305763, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 285, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (245.19, 77.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (288.23, 147.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 192, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (209.18, 90.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725276 sequences (100000193 bp) in 2.350 CPU sec, 2.498 real sec...
[M::process] read 726684 sequences (100000013 bp) in 2.050 CPU sec, 2.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305559, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 137, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (156.50, 64.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (289.09, 146.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 155, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (181.58, 90.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 602)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724896 reads in 173.644 CPU sec, 5.509 real sec
[M::mem_process_seqs] Processed 724366 reads in 100.974 CPU sec, 3.192 real sec
sorting block 18, #reads = 1102310 mem = 536881039 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306069, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 214, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (198.57, 72.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (287.24, 146.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726602 sequences (100000119 bp) in 2.495 CPU sec, 2.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306485, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 218, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (226.50, 77.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.54, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726800 sequences (100000033 bp) in 1.840 CPU sec, 1.840 real sec...
[M::mem_process_seqs] Processed 725276 reads in 175.641 CPU sec, 5.589 real sec
sorting block 19, #reads = 1101749 mem = 536880900 peak = 965054464
[M::mem_process_seqs] Processed 726684 reads in 100.099 CPU sec, 3.162 real sec
sorting block 20, #reads = 1103322 mem = 536878475 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306408, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 183, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (220.82, 143.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.70, 145.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 203, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486)
[M::mem_pestat] mean and std.dev: (201.43, 90.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 591)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728190 sequences (100000181 bp) in 2.509 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306287, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 173, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (185.60, 53.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (281.89, 144.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 174, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (184.94, 64.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727192 sequences (100000014 bp) in 1.957 CPU sec, 1.958 real sec...
[M::mem_process_seqs] Processed 726602 reads in 171.840 CPU sec, 5.458 real sec
[M::mem_process_seqs] Processed 726800 reads in 96.049 CPU sec, 3.030 real sec
sorting block 21, #reads = 1102894 mem = 536877384 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307312, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 222, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (218.06, 99.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 616)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 254, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (277.53, 142.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 185, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (242.72, 176.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726520 sequences (100000132 bp) in 2.099 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306747, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 192, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (253.53, 148.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (280.23, 143.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 239, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (214.62, 68.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 741)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726244 sequences (100000195 bp) in 2.090 CPU sec, 2.096 real sec...
[M::mem_process_seqs] Processed 728190 reads in 164.948 CPU sec, 5.238 real sec
[M::mem_process_seqs] Processed 727192 reads in 93.320 CPU sec, 2.948 real sec
sorting block 22, #reads = 1103750 mem = 536877700 peak = 965054464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306072, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 204, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (217.33, 61.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (281.49, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 213, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (229.50, 74.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725964 sequences (100000093 bp) in 2.031 CPU sec, 2.171 real sec...
[M::process] read 726154 sequences (100000143 bp) in 1.400 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306766, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 177, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (185.46, 75.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (282.94, 144.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 190, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 399)
[M::mem_pestat] mean and std.dev: (167.73, 48.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 478)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726520 reads in 157.452 CPU sec, 5.010 real sec
sorting block 23, #reads = 1103534 mem = 536881433 peak = 965054464
[M::mem_process_seqs] Processed 726244 reads in 90.031 CPU sec, 2.868 real sec
sorting block 24, #reads = 1103115 mem = 536877473 peak = 1003565056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306345, 11, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 183, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 342)
[M::mem_pestat] mean and std.dev: (181.04, 63.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 435)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (284.85, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 58, 3275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9749)
[M::mem_pestat] mean and std.dev: (920.36, 1442.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12986)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 224, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (244.39, 114.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 725710 sequences (100000271 bp) in 1.992 CPU sec, 2.155 real sec...
[M::process] read 725594 sequences (100000235 bp) in 1.535 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306039, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 206, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (187.83, 73.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (285.28, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 186, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 380)
[M::mem_pestat] mean and std.dev: (188.10, 47.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725964 reads in 155.607 CPU sec, 4.949 real sec
[M::process] read 725084 sequences (100000098 bp) in 1.665 CPU sec, 1.672 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305879, 0, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 177, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (224.62, 115.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.49, 146.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 225, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (206.95, 78.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725710 reads in 82.519 CPU sec, 2.620 real sec
sorting block 25, #reads = 1103063 mem = 536879054 peak = 1003565056
[M::mem_process_seqs] Processed 726154 reads in 198.523 CPU sec, 6.309 real sec
sorting block 26, #reads = 1102457 mem = 536877253 peak = 1003565056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306317, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 170, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (210.95, 106.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (287.01, 147.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 144, 161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (83, 213)
[M::mem_pestat] mean and std.dev: (142.09, 22.06)
[M::mem_pestat] low and high boundaries for proper pairs: (54, 239)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724872 sequences (100000229 bp) in 1.743 CPU sec, 1.772 real sec...
[M::process] read 725028 sequences (100000170 bp) in 1.960 CPU sec, 1.965 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305384, 2, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 127, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 292)
[M::mem_pestat] mean and std.dev: (138.00, 37.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 348)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (288.60, 148.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 195, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (182.87, 48.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725594 reads in 164.464 CPU sec, 5.206 real sec
[M::mem_process_seqs] Processed 725084 reads in 90.739 CPU sec, 2.869 real sec
sorting block 27, #reads = 1102596 mem = 536880584 peak = 1003565056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 305578, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 211, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (186.08, 71.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (288.14, 147.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 299, 1144)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3080)
[M::mem_pestat] mean and std.dev: (549.00, 485.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4048)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724126 sequences (100000190 bp) in 2.439 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305823, 10, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 235, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (235.91, 98.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (288.76, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 78, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173)
[M::mem_pestat] mean and std.dev: (71.70, 22.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 214)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 222, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528)
[M::mem_pestat] mean and std.dev: (217.44, 48.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 647)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 723646 sequences (100000014 bp) in 2.030 CPU sec, 2.028 real sec...
[M::mem_process_seqs] Processed 724872 reads in 158.499 CPU sec, 5.009 real sec
[M::mem_process_seqs] Processed 725028 reads in 93.534 CPU sec, 2.949 real sec
sorting block 28, #reads = 1102109 mem = 536877828 peak = 1003565056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 305482, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 180, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (207.71, 100.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (291.35, 147.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 235, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 414)
[M::mem_pestat] mean and std.dev: (215.25, 61.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723470 sequences (100000215 bp) in 2.369 CPU sec, 2.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 304861, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 192, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (205.34, 78.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (293.26, 148.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 215, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (273.42, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724126 reads in 159.005 CPU sec, 5.043 real sec
[M::process] read 725714 sequences (100000255 bp) in 2.028 CPU sec, 2.031 real sec...
[M::mem_process_seqs] Processed 723646 reads in 92.311 CPU sec, 2.910 real sec
sorting block 29, #reads = 1102138 mem = 536876444 peak = 1003565056
sorting block 30, #reads = 1100963 mem = 536878061 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304918, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 158, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (159.10, 43.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 501)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 270, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (292.71, 148.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 174, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324)
[M::mem_pestat] mean and std.dev: (153.75, 29.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 389)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725952 sequences (100000123 bp) in 2.377 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305429, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 213, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (111, 281)
[M::mem_pestat] mean and std.dev: (196.25, 26.64)
[M::mem_pestat] low and high boundaries for proper pairs: (77, 315)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (285.09, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 180, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450)
[M::mem_pestat] mean and std.dev: (185.60, 60.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 554)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723470 reads in 156.935 CPU sec, 4.981 real sec
[M::mem_process_seqs] Processed 725714 reads in 91.815 CPU sec, 2.889 real sec
[M::process] read 726026 sequences (100000228 bp) in 2.045 CPU sec, 2.057 real sec...
sorting block 31, #reads = 1101252 mem = 536878185 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306184, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 267, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (233.23, 69.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 684)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (285.22, 146.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 247, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 475)
[M::mem_pestat] mean and std.dev: (258.81, 84.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726812 sequences (100000015 bp) in 2.671 CPU sec, 2.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 306152, 6, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.44, 145.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 188, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (197.07, 67.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725952 reads in 157.389 CPU sec, 5.000 real sec
[M::mem_process_seqs] Processed 726026 reads in 92.524 CPU sec, 2.929 real sec
[M::process] read 727358 sequences (100000104 bp) in 2.697 CPU sec, 2.811 real sec...
sorting block 32, #reads = 1102778 mem = 536877290 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305848, 1, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 179, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544)
[M::mem_pestat] mean and std.dev: (197.26, 68.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (281.70, 144.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 201, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (233.11, 99.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726694 sequences (100000041 bp) in 2.339 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307016, 1, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 182, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (223.20, 101.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (279.79, 143.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 249, 805)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2089)
[M::mem_pestat] mean and std.dev: (318.20, 249.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2731)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726812 reads in 157.715 CPU sec, 5.023 real sec
[M::mem_process_seqs] Processed 727358 reads in 90.234 CPU sec, 2.845 real sec
[M::process] read 726586 sequences (100000197 bp) in 1.875 CPU sec, 1.929 real sec...
sorting block 33, #reads = 1103021 mem = 536879246 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 306449, 3, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.83, 145.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 183, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (209.88, 97.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] skip orientation RR
sorting block 34, #reads = 1103417 mem = 536878220 peak = 1014087680
[M::process] read 725908 sequences (100000153 bp) in 2.416 CPU sec, 2.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306353, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 162, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351)
[M::mem_pestat] mean and std.dev: (168.70, 52.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.47, 145.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 210, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 380)
[M::mem_pestat] mean and std.dev: (202.68, 62.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726694 reads in 156.775 CPU sec, 4.992 real sec
[M::mem_process_seqs] Processed 726586 reads in 91.103 CPU sec, 2.876 real sec
[M::process] read 725712 sequences (100000176 bp) in 2.794 CPU sec, 2.983 real sec...
sorting block 35, #reads = 1103500 mem = 536879825 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306171, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 151, 162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (108, 198)
[M::mem_pestat] mean and std.dev: (142.36, 15.51)
[M::mem_pestat] low and high boundaries for proper pairs: (80, 216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (284.95, 146.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 258, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 513)
[M::mem_pestat] mean and std.dev: (253.00, 59.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725894 sequences (100000160 bp) in 2.964 CPU sec, 3.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305832, 10, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 170, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (192.53, 69.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.90, 146.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (53, 96, 96)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182)
[M::mem_pestat] mean and std.dev: (67.89, 31.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 225)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 220, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 337)
[M::mem_pestat] mean and std.dev: (205.88, 63.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 459)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725908 reads in 155.383 CPU sec, 4.958 real sec
[M::mem_process_seqs] Processed 725712 reads in 91.468 CPU sec, 2.888 real sec
sorting block 36, #reads = 1103135 mem = 536881017 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306061, 0, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 185, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (199.00, 93.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (286.32, 147.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 137, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (168.54, 95.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725894 reads in 83.638 CPU sec, 2.774 real sec
[M::process] read 725594 sequences (100000001 bp) in 3.425 CPU sec, 3.732 real sec...
sorting block 37, #reads = 1102599 mem = 536881910 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306020, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 178, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (200.88, 85.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (287.14, 147.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 232, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (203.44, 84.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725594 reads in 83.491 CPU sec, 2.758 real sec
[M::process] read 725562 sequences (100000097 bp) in 3.572 CPU sec, 3.998 real sec...
sorting block 38, #reads = 1102901 mem = 536878866 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 305649, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (283, 289, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (313.22, 119.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (287.71, 148.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 205, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (266.40, 156.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725562 reads in 86.742 CPU sec, 2.790 real sec
[M::process] read 725344 sequences (100000257 bp) in 2.855 CPU sec, 3.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305724, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 181, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (182.00, 55.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (288.12, 147.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 173, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344)
[M::mem_pestat] mean and std.dev: (181.00, 69.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 459)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725344 reads in 84.801 CPU sec, 2.745 real sec
sorting block 39, #reads = 1102163 mem = 536880815 peak = 1014087680
[M::process] read 725146 sequences (100000206 bp) in 3.293 CPU sec, 3.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305651, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 190, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (215.33, 103.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (289.33, 149.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 234, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (306.67, 185.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725146 reads in 82.065 CPU sec, 2.649 real sec
[M::process] read 724858 sequences (100000144 bp) in 2.590 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305454, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 210, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (209.14, 74.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (290.62, 149.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 192, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (248.73, 110.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724858 reads in 82.863 CPU sec, 2.682 real sec
sorting block 40, #reads = 1102648 mem = 536877844 peak = 1014087680
[M::process] read 724296 sequences (100000101 bp) in 3.633 CPU sec, 4.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305350, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 169, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 337)
[M::mem_pestat] mean and std.dev: (179.69, 63.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (291.63, 149.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 184, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (183.33, 94.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 723406 sequences (100000013 bp) in 2.189 CPU sec, 2.427 real sec...
[M::mem_process_seqs] Processed 724296 reads in 80.894 CPU sec, 2.610 real sec
sorting block 41, #reads = 1102039 mem = 536879238 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304544, 5, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 259, 640)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1608)
[M::mem_pestat] mean and std.dev: (255.91, 177.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2092)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 270, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (293.54, 149.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 207, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (215.25, 107.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 723406 reads in 81.329 CPU sec, 2.614 real sec
[M::process] read 725182 sequences (100000072 bp) in 2.935 CPU sec, 3.082 real sec...
sorting block 42, #reads = 1101105 mem = 536877345 peak = 1014087680
[M::process] read 725676 sequences (100000154 bp) in 2.033 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305120, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 157, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (254.75, 196.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 265, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (288.39, 147.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 233, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (225.58, 65.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725628 sequences (100000231 bp) in 1.760 CPU sec, 1.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306097, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 176, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 378)
[M::mem_pestat] mean and std.dev: (194.14, 51.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.47, 145.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 227, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (82, 347)
[M::mem_pestat] mean and std.dev: (198.15, 51.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 403)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725182 reads in 150.295 CPU sec, 4.722 real sec
[M::mem_process_seqs] Processed 725676 reads in 88.835 CPU sec, 2.788 real sec
[M::process] read 726358 sequences (100000025 bp) in 2.372 CPU sec, 2.461 real sec...
sorting block 43, #reads = 1101839 mem = 536879357 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305978, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 192, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (218.79, 91.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.37, 145.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 193, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482)
[M::mem_pestat] mean and std.dev: (181.21, 63.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 593)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729898 sequences (100000063 bp) in 2.810 CPU sec, 2.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306207, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 183, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (192.25, 65.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (283.42, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 222, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (231.81, 121.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725628 reads in 153.713 CPU sec, 4.892 real sec
[M::mem_process_seqs] Processed 726358 reads in 91.273 CPU sec, 2.875 real sec
sorting block 44, #reads = 1102815 mem = 536880010 peak = 1014087680
[M::process] read 728236 sequences (100000100 bp) in 2.351 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 307551, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 152, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 326)
[M::mem_pestat] mean and std.dev: (170.77, 54.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 389)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 247, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (273.83, 143.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 189, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (198.40, 67.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 507)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727680 sequences (100000145 bp) in 1.906 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306930, 1, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 147, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (174.53, 84.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (278.75, 144.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 258, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (271.00, 94.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729898 reads in 156.944 CPU sec, 4.972 real sec
[M::mem_process_seqs] Processed 728236 reads in 89.365 CPU sec, 2.810 real sec
[M::process] read 727400 sequences (100000138 bp) in 1.778 CPU sec, 1.802 real sec...
sorting block 45, #reads = 1104335 mem = 536879266 peak = 1014087680
sorting block 46, #reads = 1104487 mem = 536879084 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306427, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 202, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (240.92, 107.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (278.80, 143.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 238, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (224.31, 97.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727552 sequences (100000143 bp) in 2.360 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306570, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 204, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (211.33, 91.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (281.11, 145.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 239, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (199.62, 93.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727680 reads in 158.990 CPU sec, 5.049 real sec
[M::mem_process_seqs] Processed 727400 reads in 91.996 CPU sec, 2.891 real sec
[M::process] read 727122 sequences (100000222 bp) in 2.370 CPU sec, 2.401 real sec...
sorting block 47, #reads = 1104366 mem = 536880563 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 307051, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 220, 841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2227)
[M::mem_pestat] mean and std.dev: (240.57, 181.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2920)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.76, 145.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (252.27, 156.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727248 sequences (100000109 bp) in 2.393 CPU sec, 2.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 306990, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 175, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373)
[M::mem_pestat] mean and std.dev: (168.06, 45.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 453)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (281.33, 144.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 235, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (258.85, 75.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727552 reads in 160.268 CPU sec, 5.093 real sec
[M::process] read 727808 sequences (100000224 bp) in 1.550 CPU sec, 1.547 real sec...
[M::mem_process_seqs] Processed 727122 reads in 92.591 CPU sec, 2.914 real sec
sorting block 48, #reads = 1103655 mem = 536878954 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 306475, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 212, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (202.13, 67.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (280.00, 144.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728566 sequences (100000067 bp) in 2.608 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 307331, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 197, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (196.32, 80.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (278.02, 142.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 155, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 318)
[M::mem_pestat] mean and std.dev: (162.06, 42.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 378)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727248 reads in 158.915 CPU sec, 5.044 real sec
[M::mem_process_seqs] Processed 727808 reads in 92.606 CPU sec, 2.910 real sec
[M::process] read 728324 sequences (100000233 bp) in 2.227 CPU sec, 2.255 real sec...
sorting block 49, #reads = 1104130 mem = 536880074 peak = 1014087680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 307497, 11, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 182, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443)
[M::mem_pestat] mean and std.dev: (187.91, 55.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (275.44, 142.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 104, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (189.45, 167.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 150, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393)
[M::mem_pestat] mean and std.dev: (169.53, 52.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 478)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 728088 sequences (100000279 bp) in 2.087 CPU sec, 2.116 real sec...
[M::process] read 727462 sequences (100000120 bp) in 1.610 CPU sec, 1.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 307182, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 167, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 362)
[M::mem_pestat] mean and std.dev: (182.20, 74.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 482)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.89, 142.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 184, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (197.52, 79.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728566 reads in 162.458 CPU sec, 5.164 real sec
sorting block 50, #reads = 1104759 mem = 536876759 peak = 1014087680
[M::mem_process_seqs] Processed 728324 reads in 93.964 CPU sec, 2.972 real sec
sorting block 51, #reads = 1104915 mem = 536879187 peak = 1046528000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 307273, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 157, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (213.12, 129.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (275.78, 142.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 246, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (238.33, 137.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727456 sequences (100000098 bp) in 2.521 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306703, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 166, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (183.81, 109.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (277.56, 142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 254, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (241.52, 112.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728726 sequences (100000270 bp) in 1.930 CPU sec, 1.931 real sec...
[M::mem_process_seqs] Processed 728088 reads in 162.859 CPU sec, 5.170 real sec
[M::mem_process_seqs] Processed 727462 reads in 94.413 CPU sec, 2.980 real sec
sorting block 52, #reads = 1105112 mem = 536878609 peak = 1046528000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306580, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 230, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (211.31, 61.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (279.29, 143.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 728390 sequences (100000012 bp) in 2.305 CPU sec, 2.377 real sec...
[M::process] read 728806 sequences (100000109 bp) in 1.591 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 307227, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 167, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (240.58, 166.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (276.39, 143.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 186, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (186.27, 85.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727456 reads in 161.141 CPU sec, 5.121 real sec
[M::mem_process_seqs] Processed 728726 reads in 93.614 CPU sec, 2.956 real sec
sorting block 53, #reads = 1104203 mem = 536877775 peak = 1046528000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306769, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 240, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (214.68, 66.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (277.63, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 179, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (165.56, 63.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729264 sequences (100000233 bp) in 2.842 CPU sec, 2.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 307799, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 143, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (152.07, 62.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 251, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (277.34, 144.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 225, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 496)
[M::mem_pestat] mean and std.dev: (232.75, 59.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728390 reads in 159.160 CPU sec, 5.042 real sec
sorting block 54, #reads = 1105337 mem = 536877168 peak = 1046528000
[M::mem_process_seqs] Processed 728806 reads in 89.774 CPU sec, 2.816 real sec
[M::process] read 728568 sequences (100000085 bp) in 2.252 CPU sec, 2.265 real sec...
sorting block 55, #reads = 1105277 mem = 536878577 peak = 1056362496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 307395, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 249, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (276.45, 145.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 172, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (183.17, 79.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] skip orientation RR
[M::process] read 727834 sequences (100000263 bp) in 2.278 CPU sec, 2.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306778, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 163, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (164.39, 63.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 253, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (277.85, 143.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 203, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (228.00, 80.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729264 reads in 146.517 CPU sec, 4.643 real sec
[M::mem_process_seqs] Processed 728568 reads in 84.093 CPU sec, 2.637 real sec
[M::process] read 727430 sequences (100000256 bp) in 2.169 CPU sec, 2.184 real sec...
sorting block 56, #reads = 1104851 mem = 536878411 peak = 1056362496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 307229, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 161, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 352)
[M::mem_pestat] mean and std.dev: (165.64, 42.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 417)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.17, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 726748 sequences (100000038 bp) in 2.514 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306360, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 199, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (193.45, 83.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (280.68, 145.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 215, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (56, 356)
[M::mem_pestat] mean and std.dev: (196.19, 47.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727834 reads in 148.778 CPU sec, 4.721 real sec
[M::mem_process_seqs] Processed 727430 reads in 86.833 CPU sec, 2.730 real sec
[M::process] read 726730 sequences (100000056 bp) in 1.854 CPU sec, 1.869 real sec...
sorting block 57, #reads = 1104055 mem = 536880550 peak = 1056362496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 306469, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 186, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (205.67, 88.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (281.95, 145.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 140, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (204.70, 143.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726704 sequences (100000207 bp) in 2.611 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 306201, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 209, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (217.06, 72.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.69, 145.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 189, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 272)
[M::mem_pestat] mean and std.dev: (201.83, 20.70)
[M::mem_pestat] low and high boundaries for proper pairs: (98, 301)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726748 reads in 147.373 CPU sec, 4.677 real sec
sorting block 58, #reads = 1103559 mem = 536877181 peak = 1056362496
[M::mem_process_seqs] Processed 726730 reads in 84.604 CPU sec, 2.656 real sec
[M::process] read 726690 sequences (100000125 bp) in 2.605 CPU sec, 2.661 real sec...
sorting block 59, #reads = 1103214 mem = 536879963 peak = 1056362496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306587, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 207, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 358)
[M::mem_pestat] mean and std.dev: (201.50, 45.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 420)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (282.66, 145.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 158, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (187.53, 65.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726626 sequences (100000165 bp) in 2.369 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306788, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 194, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459)
[M::mem_pestat] mean and std.dev: (192.95, 63.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (282.90, 146.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 179, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (176.47, 52.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726704 reads in 157.653 CPU sec, 5.010 real sec
[M::mem_process_seqs] Processed 726690 reads in 92.014 CPU sec, 2.903 real sec
[M::process] read 726624 sequences (100000026 bp) in 2.425 CPU sec, 2.515 real sec...
sorting block 60, #reads = 1102775 mem = 536876862 peak = 1056362496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306060, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 225, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (238.88, 153.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.37, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (251.77, 87.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726458 sequences (100000115 bp) in 2.657 CPU sec, 2.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 306256, 9, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 136, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414)
[M::mem_pestat] mean and std.dev: (150.38, 50.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (282.51, 145.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 249, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (226.36, 101.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726626 reads in 161.567 CPU sec, 5.134 real sec
[M::mem_process_seqs] Processed 726624 reads in 94.454 CPU sec, 2.974 real sec
sorting block 61, #reads = 1103714 mem = 536878466 peak = 1056362496
[M::process] read 725620 sequences (100000197 bp) in 2.693 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306355, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 170, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470)
[M::mem_pestat] mean and std.dev: (182.80, 69.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 582)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.69, 146.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 211, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (237.82, 80.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725234 sequences (100000136 bp) in 2.443 CPU sec, 2.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305600, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 139, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (164.47, 64.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (285.43, 146.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 217, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (205.24, 71.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726458 reads in 160.140 CPU sec, 5.083 real sec
sorting block 62, #reads = 1103009 mem = 536878858 peak = 1056362496
[M::mem_process_seqs] Processed 725620 reads in 92.989 CPU sec, 2.922 real sec
[M::process] read 725644 sequences (100000151 bp) in 1.715 CPU sec, 1.731 real sec...
sorting block 63, #reads = 1102960 mem = 536878143 peak = 1060036608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 305062, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 192, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (198.92, 70.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.97, 146.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 208, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873)
[M::mem_pestat] mean and std.dev: (385.00, 412.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2441)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727652 sequences (100000148 bp) in 2.825 CPU sec, 2.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 305574, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 209, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (199.62, 69.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (287.20, 147.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 246, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (206.65, 66.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725234 reads in 160.479 CPU sec, 5.104 real sec
[M::mem_process_seqs] Processed 725644 reads in 93.735 CPU sec, 2.955 real sec
[M::process] read 727378 sequences (100000071 bp) in 2.648 CPU sec, 2.682 real sec...
sorting block 64, #reads = 1101556 mem = 536878623 peak = 1060036608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 306841, 1, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 233, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504)
[M::mem_pestat] mean and std.dev: (195.20, 78.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 254, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (279.45, 144.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 189, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (205.53, 69.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 728)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 728342 sequences (100000130 bp) in 2.726 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 306659, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 191, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470)
[M::mem_pestat] mean and std.dev: (200.92, 75.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 575)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 256, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (281.14, 145.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 194, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (185.46, 57.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 559)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727652 reads in 161.341 CPU sec, 5.120 real sec
[M::mem_process_seqs] Processed 727378 reads in 93.760 CPU sec, 2.944 real sec
sorting block 65, #reads = 1103362 mem = 536880620 peak = 1060036608
[M::process] read 729002 sequences (100000113 bp) in 2.507 CPU sec, 2.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 307461, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 171, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (203.90, 99.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 252, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (279.06, 145.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 180, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (200.12, 98.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727728 sequences (100000220 bp) in 2.039 CPU sec, 2.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 307530, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 185, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (101, 246)
[M::mem_pestat] mean and std.dev: (163.33, 35.96)
[M::mem_pestat] low and high boundaries for proper pairs: (19, 307)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (277.49, 145.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 153, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (160.56, 68.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 728342 reads in 152.304 CPU sec, 4.849 real sec
[M::mem_process_seqs] Processed 729002 reads in 86.637 CPU sec, 2.728 real sec
[M::process] read 726872 sequences (100000026 bp) in 1.753 CPU sec, 1.772 real sec...
sorting block 66, #reads = 1104338 mem = 536877818 peak = 1060036608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 306506, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 181, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (200.90, 69.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 255, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (280.36, 144.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 230, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (219.20, 83.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 67, #reads = 1104864 mem = 536880649 peak = 1068318720
[M::process] read 726640 sequences (100000174 bp) in 2.853 CPU sec, 3.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306208, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 190, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (223.11, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (282.14, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 169, 1442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4018)
[M::mem_pestat] mean and std.dev: (604.65, 823.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5306)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727728 reads in 148.270 CPU sec, 4.723 real sec
[M::mem_process_seqs] Processed 726872 reads in 85.926 CPU sec, 2.708 real sec
[M::process] read 726178 sequences (100000154 bp) in 2.338 CPU sec, 2.385 real sec...
sorting block 68, #reads = 1104131 mem = 536881842 peak = 1068318720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 306076, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 153, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 294)
[M::mem_pestat] mean and std.dev: (151.20, 39.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 352)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.99, 144.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 172, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (209.37, 93.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726234 sequences (100000176 bp) in 2.707 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306220, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 213, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (213.25, 75.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (283.79, 145.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 726640 reads in 145.365 CPU sec, 4.627 real sec
[M::mem_process_seqs] Processed 726178 reads in 83.057 CPU sec, 2.619 real sec
sorting block 69, #reads = 1103199 mem = 536877878 peak = 1068318720
[M::process] read 725958 sequences (100000123 bp) in 2.465 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306081, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 169, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465)
[M::mem_pestat] mean and std.dev: (200.05, 66.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (284.17, 144.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 175, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (175.63, 63.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725958 reads in 75.952 CPU sec, 2.430 real sec
[M::process] read 725914 sequences (100000150 bp) in 2.667 CPU sec, 2.749 real sec...
sorting block 70, #reads = 1103186 mem = 536878716 peak = 1068318720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305844, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 173, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (167.43, 52.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 261, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (285.18, 145.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 194, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (169.46, 49.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306105, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 182, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (188.73, 83.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (283.95, 145.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 197, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465)
[M::mem_pestat] mean and std.dev: (206.00, 87.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725914 reads in 72.856 CPU sec, 2.365 real sec
[M::mem_process_seqs] Processed 726234 reads in 221.625 CPU sec, 7.164 real sec
[M::process] read 725778 sequences (100000133 bp) in 3.164 CPU sec, 3.317 real sec...
sorting block 71, #reads = 1103312 mem = 536881110 peak = 1068318720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305895, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 171, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 359)
[M::mem_pestat] mean and std.dev: (166.09, 46.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 429)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 262, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.10, 146.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 155, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (178.89, 62.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725778 reads in 81.226 CPU sec, 2.640 real sec
[M::process] read 725454 sequences (100000055 bp) in 3.003 CPU sec, 3.290 real sec...
sorting block 72, #reads = 1101849 mem = 536880079 peak = 1068318720
[M::process] read 725834 sequences (100000137 bp) in 2.052 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 305464, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 200, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (213.58, 82.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (287.09, 147.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 190, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (89, 294)
[M::mem_pestat] mean and std.dev: (185.90, 40.28)
[M::mem_pestat] low and high boundaries for proper pairs: (25, 347)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725238 sequences (100000162 bp) in 1.570 CPU sec, 1.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 306078, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 219, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (212.26, 98.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (287.39, 148.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (108, 204, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (200.86, 101.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 692)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725454 reads in 143.378 CPU sec, 4.507 real sec
[M::mem_process_seqs] Processed 725834 reads in 83.408 CPU sec, 2.618 real sec
sorting block 73, #reads = 1101651 mem = 536878110 peak = 1068318720
[M::process] read 724944 sequences (100000110 bp) in 2.446 CPU sec, 2.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305648, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 149, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (167.93, 54.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 563)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 264, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (288.63, 148.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (93, 130, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (189.37, 115.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 729)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724714 sequences (100000118 bp) in 2.295 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 305969, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 254, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (188.50, 85.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (290.40, 148.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 219, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664)
[M::mem_pestat] mean and std.dev: (298.15, 198.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2161)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725238 reads in 141.160 CPU sec, 4.487 real sec
[M::mem_process_seqs] Processed 724944 reads in 82.967 CPU sec, 2.612 real sec
sorting block 74, #reads = 1102190 mem = 536878165 peak = 1068318720
[M::process] read 726608 sequences (100000267 bp) in 2.269 CPU sec, 2.372 real sec...
sorting block 75, #reads = 1102177 mem = 536877934 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305255, 8, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 170, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (197.91, 93.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (290.99, 149.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 206, 801)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105)
[M::mem_pestat] mean and std.dev: (376.24, 297.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2757)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 727268 sequences (100000087 bp) in 2.030 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306268, 15, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 179, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390)
[M::mem_pestat] mean and std.dev: (190.00, 68.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (284.67, 147.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 266, 820)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2284)
[M::mem_pestat] mean and std.dev: (377.73, 311.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3016)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 224, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (203.95, 72.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 724714 reads in 144.483 CPU sec, 4.583 real sec
[M::mem_process_seqs] Processed 726608 reads in 83.017 CPU sec, 2.609 real sec
[M::process] read 727844 sequences (100000204 bp) in 1.924 CPU sec, 1.941 real sec...
sorting block 76, #reads = 1102015 mem = 536879915 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 306544, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 234, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444)
[M::mem_pestat] mean and std.dev: (222.38, 78.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 256, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (282.77, 147.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 238, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (226.50, 118.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 729132 sequences (100000035 bp) in 2.601 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306958, 0, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 195, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 403)
[M::mem_pestat] mean and std.dev: (200.00, 67.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (280.40, 146.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 209, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (214.59, 106.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 727268 reads in 141.639 CPU sec, 4.508 real sec
[M::mem_process_seqs] Processed 727844 reads in 83.960 CPU sec, 2.641 real sec
sorting block 77, #reads = 1103039 mem = 536878727 peak = 1107705856
[M::process] read 728278 sequences (100000167 bp) in 2.326 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 307543, 2, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 221, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (211.77, 55.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 659)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 250, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (277.98, 146.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 213, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437)
[M::mem_pestat] mean and std.dev: (198.29, 64.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 534)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726974 sequences (100000254 bp) in 2.604 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306923, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 201, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (204.95, 89.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 254, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (280.71, 146.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 195, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (167.07, 52.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 522)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 729132 reads in 150.227 CPU sec, 4.792 real sec
[M::mem_process_seqs] Processed 728278 reads in 86.148 CPU sec, 2.720 real sec
sorting block 78, #reads = 1103508 mem = 536879954 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306502, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 271, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (234.50, 120.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 258, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (283.38, 146.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 252, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (228.27, 93.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726564 sequences (100000208 bp) in 3.068 CPU sec, 3.316 real sec...
[M::mem_process_seqs] Processed 726974 reads in 77.834 CPU sec, 2.560 real sec
sorting block 79, #reads = 1105185 mem = 536877384 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306430, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 174, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (193.93, 92.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (283.68, 145.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 187, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (166.82, 50.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 635)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726564 reads in 79.723 CPU sec, 2.606 real sec
sorting block 80, #reads = 1103438 mem = 536878115 peak = 1107705856
[M::process] read 726516 sequences (100000162 bp) in 3.326 CPU sec, 3.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 306172, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 170, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377)
[M::mem_pestat] mean and std.dev: (171.57, 52.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 457)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 259, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (284.60, 146.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 214, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (199.33, 71.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726516 reads in 80.598 CPU sec, 2.610 real sec
[M::process] read 726612 sequences (100000031 bp) in 2.855 CPU sec, 3.043 real sec...
sorting block 81, #reads = 1103422 mem = 536880480 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 306075, 0, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 201, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (196.29, 73.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 260, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (285.12, 147.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 170, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (160.77, 47.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725972 sequences (100000159 bp) in 2.315 CPU sec, 2.399 real sec...
[M::mem_process_seqs] Processed 726612 reads in 80.068 CPU sec, 2.574 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305871, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 180, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (172.79, 41.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 260, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (284.74, 146.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 185, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (171.87, 50.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725972 reads in 79.725 CPU sec, 2.571 real sec
[M::process] read 725946 sequences (100000239 bp) in 2.824 CPU sec, 3.018 real sec...
sorting block 82, #reads = 1103362 mem = 536878211 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305777, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 180, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (177.00, 69.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 262, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (286.26, 147.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (213.75, 68.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725946 reads in 73.814 CPU sec, 2.399 real sec
[M::process] read 725524 sequences (100000136 bp) in 2.516 CPU sec, 2.632 real sec...
sorting block 83, #reads = 1101874 mem = 536876386 peak = 1107705856
[M::process] read 725200 sequences (100000114 bp) in 2.033 CPU sec, 2.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305699, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 147, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351)
[M::mem_pestat] mean and std.dev: (160.82, 59.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 263, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (286.76, 146.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 194, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (195.94, 77.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 725226 sequences (100000163 bp) in 1.470 CPU sec, 1.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 305088, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 193, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (248.78, 156.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (287.88, 147.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 199, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (200.33, 73.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725524 reads in 143.698 CPU sec, 4.519 real sec
[M::mem_process_seqs] Processed 725200 reads in 83.547 CPU sec, 2.628 real sec
[M::process] read 725168 sequences (100000170 bp) in 1.712 CPU sec, 1.729 real sec...
sorting block 84, #reads = 1102488 mem = 536879961 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305408, 0, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 254, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (233.91, 73.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 264, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (288.78, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 216, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (234.14, 107.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 724400 sequences (100000012 bp) in 2.511 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305163, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347)
[M::mem_pestat] mean and std.dev: (166.67, 42.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 417)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 266, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (289.63, 148.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 158, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (158.67, 45.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 501)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 725226 reads in 148.907 CPU sec, 4.732 real sec
[M::mem_process_seqs] Processed 725168 reads in 87.871 CPU sec, 2.769 real sec
sorting block 85, #reads = 1102500 mem = 536879186 peak = 1107705856
[M::process] read 724670 sequences (100000104 bp) in 2.374 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 305350, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 150, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (226.08, 148.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (291.15, 148.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 499)
[M::mem_pestat] mean and std.dev: (208.30, 54.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 595)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 726386 sequences (100000188 bp) in 1.974 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 305324, 10, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 217, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (245.05, 128.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 267, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (291.72, 149.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (77, 107, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (102.75, 65.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 163, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 328)
[M::mem_pestat] mean and std.dev: (154.42, 26.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 393)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 726882 sequences (100000172 bp) in 1.470 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 724400 reads in 148.915 CPU sec, 4.718 real sec
[M::mem_process_seqs] Processed 724670 reads in 85.595 CPU sec, 2.699 real sec
sorting block 86, #reads = 1101934 mem = 536880072 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305862, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 168, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 290)
[M::mem_pestat] mean and std.dev: (162.91, 33.08)
[M::mem_pestat] low and high boundaries for proper pairs: (2, 338)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (282.81, 145.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 170, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350)
[M::mem_pestat] mean and std.dev: (164.86, 40.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 87, #reads = 1101677 mem = 536879121 peak = 1107705856
[M::process] read 726954 sequences (100000284 bp) in 2.581 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 306475, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 274, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (251.15, 77.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 257, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (281.87, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 159, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (161.09, 54.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 335444 sequences (46149244 bp) in 1.080 CPU sec, 1.084 real sec...
[M::mem_process_seqs] Processed 726386 reads in 142.623 CPU sec, 4.531 real sec
[M::mem_process_seqs] Processed 726882 reads in 80.698 CPU sec, 2.554 real sec
sorting block 88, #reads = 1103103 mem = 536878744 peak = 1107705856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 141574, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 255, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (282.23, 146.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 335444 reads in 35.742 CPU sec, 1.173 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 306881, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 150, 190)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 292)
[M::mem_pestat] mean and std.dev: (157.11, 44.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 343)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 256, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (282.50, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 290, 1300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3576)
[M::mem_pestat] mean and std.dev: (719.42, 691.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4714)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 726954 reads in 109.543 CPU sec, 4.074 real sec
sorting block 89, #reads = 1103550 mem = 536879303 peak = 1107705856
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 99148610
overall: 69686792192 mem 12836.942 user 461.043 sys 453.555 real
sorting block 90, #reads = 426140 mem = 207115781 peak = 1107705856
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.SH032_HG514.L003\tPU:L003\tSM:SH032_HG514\tLB:SH032_HG514\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.SH032_HG514_R1.fastq.gz 0001.SH032_HG514_R2.fastq.gz
[main] Real time: 453.633 sec; CPU: 13298.026 sec
uniq header count 1
merging 91 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 4419740691
output reads: 99723686
bam_mem_sort: 91 calls 50.968 user 1.101 sys 52.242 real
bam_write: 91 calls 29.320 user 1.646 sys 31.976 real
execute: 1 calls 30.167 user 1.435 sys 487.316 real
merge_files: 1 calls 30.139 user 1.407 sys 33.449 real
parse_chunk: 1447 calls 72.620 user 19.097 sys 93.083 real
read_chunk: 3917 calls 41.050 user 27.995 sys 394.282 real
sort_block: 1 calls 28.289 user 27.002 sys 376.452 real
write_chunk: 2468 calls 29.722 user 1.137 sys 32.540 real
overall: 2632257536 mem 1276.687 user 72.927 sys 487.339 real
ls: cannot access '*.cram': No such file or directory