This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o D0001483_22q.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1641.569 CPU sec, 56.823 real sec
[M::process] read 716500 sequences (100000230 bp) in 1.119 CPU sec, 1.125 real sec...
[M::process] read 715900 sequences (100000263 bp) in 1.276 CPU sec, 1.278 real sec...
[M::process] read 715758 sequences (100000088 bp) in 1.519 CPU sec, 1.520 real sec...
[M::process] read 715522 sequences (100000054 bp) in 1.390 CPU sec, 1.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304203, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (183.38, 69.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715900 reads in 80.076 CPU sec, 2.546 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304540, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 355)
[M::mem_pestat] mean and std.dev: (178.17, 63.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 432)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715670 sequences (100000024 bp) in 1.666 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304413, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (187.21, 73.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 44, 77)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225)
[M::mem_pestat] mean and std.dev: (42.73, 36.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 299)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 716500 reads in 245.345 CPU sec, 7.779 real sec
[M::mem_process_seqs] Processed 715758 reads in 103.011 CPU sec, 3.338 real sec
sorting block 0, #reads = 1092583 mem = 536877196 peak = 637693952
[M::process] read 715438 sequences (100000018 bp) in 1.922 CPU sec, 1.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303863, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.65, 79.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715406 sequences (100000156 bp) in 1.450 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303861, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.55, 78.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304145, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (189.58, 76.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715522 reads in 247.268 CPU sec, 7.989 real sec
sorting block 1, #reads = 1092709 mem = 536878323 peak = 986787840
[M::mem_process_seqs] Processed 715670 reads in 174.923 CPU sec, 5.709 real sec
[M::mem_process_seqs] Processed 715438 reads in 184.157 CPU sec, 6.052 real sec
sorting block 2, #reads = 1092018 mem = 536877623 peak = 986787840
[M::process] read 715248 sequences (100000022 bp) in 1.742 CPU sec, 1.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 304377, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (191.34, 79.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715214 sequences (100000255 bp) in 2.197 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304055, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.77, 78.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715406 reads in 181.836 CPU sec, 5.779 real sec
[M::process] read 715304 sequences (100000008 bp) in 1.705 CPU sec, 1.734 real sec...
sorting block 3, #reads = 1092370 mem = 536878945 peak = 986787840
[M::mem_process_seqs] Processed 715248 reads in 102.615 CPU sec, 3.257 real sec
sorting block 4, #reads = 1092172 mem = 536876700 peak = 998096896
[M::process] read 715386 sequences (100000220 bp) in 2.127 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303830, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (191.94, 80.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715282 sequences (100000276 bp) in 1.766 CPU sec, 1.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303892, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (192.59, 81.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304147, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.72, 79.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 72, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285)
[M::mem_pestat] mean and std.dev: (189.00, 225.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1710)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715214 reads in 252.151 CPU sec, 8.113 real sec
[M::mem_process_seqs] Processed 715304 reads in 177.376 CPU sec, 5.753 real sec
[M::mem_process_seqs] Processed 715386 reads in 186.903 CPU sec, 6.113 real sec
sorting block 5, #reads = 1092073 mem = 536879771 peak = 998096896
sorting block 6, #reads = 1091971 mem = 536878533 peak = 1006055424
[M::process] read 715876 sequences (100000169 bp) in 2.436 CPU sec, 2.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304064, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (189.54, 76.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715858 sequences (100000194 bp) in 2.199 CPU sec, 2.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304258, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (182.09, 67.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 453)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715282 reads in 184.798 CPU sec, 5.882 real sec
[M::mem_process_seqs] Processed 715876 reads in 106.273 CPU sec, 3.374 real sec
[M::process] read 715740 sequences (100000028 bp) in 2.012 CPU sec, 2.017 real sec...
sorting block 7, #reads = 1091977 mem = 536877594 peak = 1006055424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304503, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367)
[M::mem_pestat] mean and std.dev: (181.78, 67.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715594 sequences (100000070 bp) in 2.267 CPU sec, 2.331 real sec...
[M::process] read 715668 sequences (100000150 bp) in 1.857 CPU sec, 1.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304681, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 364)
[M::mem_pestat] mean and std.dev: (181.04, 66.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715858 reads in 183.535 CPU sec, 5.844 real sec
[M::mem_process_seqs] Processed 715740 reads in 106.701 CPU sec, 3.411 real sec
sorting block 8, #reads = 1092689 mem = 536879752 peak = 1006055424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304322, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (184.29, 69.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715246 sequences (100000130 bp) in 2.582 CPU sec, 2.742 real sec...
[M::process] read 714740 sequences (100000047 bp) in 1.900 CPU sec, 1.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304641, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367)
[M::mem_pestat] mean and std.dev: (181.73, 67.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715594 reads in 181.424 CPU sec, 5.787 real sec
sorting block 9, #reads = 1093026 mem = 536880618 peak = 1006055424
[M::mem_process_seqs] Processed 715668 reads in 101.204 CPU sec, 3.227 real sec
sorting block 10, #reads = 1092672 mem = 536877010 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304077, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (191.13, 78.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714678 sequences (100000248 bp) in 2.156 CPU sec, 2.191 real sec...
[M::process] read 714124 sequences (100000221 bp) in 1.460 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304054, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (201.30, 93.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715246 reads in 172.761 CPU sec, 5.542 real sec
[M::mem_process_seqs] Processed 714740 reads in 99.692 CPU sec, 3.192 real sec
sorting block 11, #reads = 1092359 mem = 536878439 peak = 1012355072
[M::process] read 714188 sequences (100000089 bp) in 2.266 CPU sec, 2.295 real sec...
[M::process] read 715130 sequences (100000206 bp) in 1.940 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303170, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (209.17, 104.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714124 reads in 91.673 CPU sec, 2.905 real sec
sorting block 12, #reads = 1091570 mem = 536878197 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303239, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (204.34, 97.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303433, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (210.09, 105.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714678 reads in 267.637 CPU sec, 8.520 real sec
[M::process] read 716208 sequences (100000254 bp) in 1.896 CPU sec, 1.899 real sec...
[M::mem_process_seqs] Processed 714188 reads in 112.330 CPU sec, 3.624 real sec
sorting block 13, #reads = 1091399 mem = 536876617 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304183, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (200.28, 92.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715936 sequences (100000214 bp) in 2.203 CPU sec, 2.296 real sec...
[M::process] read 715866 sequences (100000011 bp) in 1.450 CPU sec, 1.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304713, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (181.83, 67.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 453)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715130 reads in 177.604 CPU sec, 5.680 real sec
[M::mem_process_seqs] Processed 716208 reads in 99.761 CPU sec, 3.200 real sec
sorting block 14, #reads = 1091180 mem = 536876699 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304334, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.89, 70.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715796 sequences (100000117 bp) in 2.727 CPU sec, 2.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304254, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.94, 74.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715524 sequences (100000214 bp) in 1.890 CPU sec, 1.891 real sec...
[M::mem_process_seqs] Processed 715936 reads in 176.195 CPU sec, 5.628 real sec
[M::mem_process_seqs] Processed 715866 reads in 100.093 CPU sec, 3.189 real sec
sorting block 15, #reads = 1092881 mem = 536877367 peak = 1012355072
sorting block 16, #reads = 1092107 mem = 536877940 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304319, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (186.11, 72.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715344 sequences (100000162 bp) in 2.561 CPU sec, 2.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304211, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (188.36, 74.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715532 sequences (100000013 bp) in 1.970 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 715796 reads in 174.548 CPU sec, 5.557 real sec
[M::mem_process_seqs] Processed 715524 reads in 99.951 CPU sec, 3.168 real sec
sorting block 17, #reads = 1092328 mem = 536878614 peak = 1012355072
[M::process] read 715286 sequences (100000227 bp) in 2.441 CPU sec, 2.478 real sec...
[M::process] read 715282 sequences (100000011 bp) in 1.640 CPU sec, 1.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304167, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433)
[M::mem_pestat] mean and std.dev: (189.03, 75.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 528)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715532 reads in 102.449 CPU sec, 3.269 real sec
sorting block 18, #reads = 1092231 mem = 536878162 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304210, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (191.09, 78.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304115, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (191.10, 78.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715344 reads in 274.000 CPU sec, 8.747 real sec
[M::process] read 715160 sequences (100000158 bp) in 1.738 CPU sec, 1.741 real sec...
[M::mem_process_seqs] Processed 715286 reads in 111.367 CPU sec, 3.592 real sec
sorting block 19, #reads = 1092525 mem = 536878581 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 304119, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (193.29, 81.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715264 sequences (100000251 bp) in 2.938 CPU sec, 3.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304019, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (193.96, 82.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715498 sequences (100000196 bp) in 1.410 CPU sec, 1.418 real sec...
[M::mem_process_seqs] Processed 715282 reads in 171.704 CPU sec, 5.487 real sec
[M::mem_process_seqs] Processed 715160 reads in 98.016 CPU sec, 3.128 real sec
sorting block 20, #reads = 1092431 mem = 536879036 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304300, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (193.04, 81.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715314 sequences (100000008 bp) in 2.682 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304430, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (188.17, 74.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715336 sequences (100000149 bp) in 1.900 CPU sec, 1.903 real sec...
[M::mem_process_seqs] Processed 715264 reads in 169.043 CPU sec, 5.365 real sec
[M::mem_process_seqs] Processed 715498 reads in 94.934 CPU sec, 3.005 real sec
sorting block 21, #reads = 1092149 mem = 536880127 peak = 1012355072
sorting block 22, #reads = 1092114 mem = 536877378 peak = 1012355072
[M::process] read 715208 sequences (100000130 bp) in 2.616 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303994, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (189.17, 76.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715376 sequences (100000206 bp) in 1.490 CPU sec, 1.495 real sec...
[M::mem_process_seqs] Processed 715336 reads in 90.863 CPU sec, 2.879 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304222, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (187.22, 73.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304330, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (191.46, 79.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714978 sequences (100000124 bp) in 1.781 CPU sec, 1.791 real sec...
[M::mem_process_seqs] Processed 715314 reads in 249.534 CPU sec, 7.958 real sec
[M::mem_process_seqs] Processed 715208 reads in 107.413 CPU sec, 3.444 real sec
sorting block 23, #reads = 1092634 mem = 536881188 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304267, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (190.16, 77.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1092516 mem = 536879334 peak = 1012355072
[M::process] read 714612 sequences (100000002 bp) in 2.629 CPU sec, 2.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303814, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.39, 85.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715376 reads in 168.209 CPU sec, 5.375 real sec
[M::mem_process_seqs] Processed 714978 reads in 94.431 CPU sec, 2.995 real sec
[M::process] read 714446 sequences (100000200 bp) in 2.153 CPU sec, 2.178 real sec...
sorting block 25, #reads = 1092391 mem = 536878842 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303204, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (203.37, 96.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714494 sequences (100000232 bp) in 2.193 CPU sec, 2.240 real sec...
[M::process] read 714216 sequences (100000009 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303497, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (204.65, 98.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714612 reads in 169.589 CPU sec, 5.407 real sec
[M::mem_process_seqs] Processed 714446 reads in 94.401 CPU sec, 3.025 real sec
sorting block 26, #reads = 1091639 mem = 536879208 peak = 1012355072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 303592, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (207.03, 101.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714212 sequences (100000039 bp) in 2.410 CPU sec, 2.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303664, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (207.24, 100.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 701)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714494 reads in 162.702 CPU sec, 5.191 real sec
[M::process] read 715894 sequences (100000105 bp) in 1.630 CPU sec, 1.632 real sec...
[M::mem_process_seqs] Processed 714216 reads in 92.497 CPU sec, 2.944 real sec
sorting block 27, #reads = 1091168 mem = 536879019 peak = 1012736000
[M::process] read 715904 sequences (100000225 bp) in 1.865 CPU sec, 1.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303084, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (208.24, 101.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716154 sequences (100000137 bp) in 1.370 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304036, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (187.14, 74.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714212 reads in 163.931 CPU sec, 5.242 real sec
sorting block 28, #reads = 1091304 mem = 536879356 peak = 1133543424
[M::mem_process_seqs] Processed 715894 reads in 91.439 CPU sec, 2.939 real sec
sorting block 29, #reads = 1090748 mem = 536879727 peak = 1133543424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304417, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (185.54, 72.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715532 sequences (100000172 bp) in 2.260 CPU sec, 2.414 real sec...
[M::process] read 715582 sequences (100000003 bp) in 1.500 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304578, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.89, 71.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715904 reads in 168.285 CPU sec, 5.386 real sec
[M::mem_process_seqs] Processed 716154 reads in 98.521 CPU sec, 3.137 real sec
sorting block 30, #reads = 1092827 mem = 536878666 peak = 1133543424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304120, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (188.11, 74.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715494 sequences (100000084 bp) in 2.602 CPU sec, 2.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304470, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (189.87, 77.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715454 sequences (100000014 bp) in 1.940 CPU sec, 1.939 real sec...
[M::mem_process_seqs] Processed 715532 reads in 167.316 CPU sec, 5.305 real sec
[M::mem_process_seqs] Processed 715582 reads in 95.827 CPU sec, 3.033 real sec
sorting block 31, #reads = 1092491 mem = 536876567 peak = 1133543424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303976, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (191.38, 79.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715354 sequences (100000035 bp) in 2.502 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304359, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (192.03, 80.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715342 sequences (100000138 bp) in 1.690 CPU sec, 1.691 real sec...
[M::mem_process_seqs] Processed 715494 reads in 173.277 CPU sec, 5.531 real sec
sorting block 32, #reads = 1092055 mem = 536879296 peak = 1133543424
[M::mem_process_seqs] Processed 715454 reads in 101.378 CPU sec, 3.218 real sec
sorting block 33, #reads = 1091998 mem = 536880941 peak = 1136881664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304737, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (192.50, 81.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 11, 67)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191)
[M::mem_pestat] mean and std.dev: (36.40, 40.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 253)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715110 sequences (100000270 bp) in 2.116 CPU sec, 2.140 real sec...
[M::process] read 715452 sequences (100000171 bp) in 1.920 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303844, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (193.83, 82.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 594, 694)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900)
[M::mem_pestat] mean and std.dev: (429.30, 331.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2503)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 715354 reads in 163.540 CPU sec, 5.198 real sec
[M::mem_process_seqs] Processed 715342 reads in 96.430 CPU sec, 3.066 real sec
sorting block 34, #reads = 1092115 mem = 536880135 peak = 1136881664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304077, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (196.06, 85.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715010 sequences (100000011 bp) in 2.775 CPU sec, 2.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304303, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (193.55, 82.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715110 reads in 167.278 CPU sec, 5.343 real sec
[M::mem_process_seqs] Processed 715452 reads in 98.814 CPU sec, 3.126 real sec
[M::process] read 714924 sequences (100000070 bp) in 2.379 CPU sec, 2.404 real sec...
sorting block 35, #reads = 1091951 mem = 536878613 peak = 1136881664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303973, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (197.29, 87.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715030 sequences (100000154 bp) in 2.189 CPU sec, 2.227 real sec...
[M::process] read 715020 sequences (100000249 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 303853, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.46, 84.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715010 reads in 163.309 CPU sec, 5.203 real sec
[M::mem_process_seqs] Processed 714924 reads in 93.466 CPU sec, 2.977 real sec
sorting block 36, #reads = 1092150 mem = 536879700 peak = 1177088000
sorting block 37, #reads = 1091888 mem = 536879757 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304190, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (198.80, 89.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715210 sequences (100000011 bp) in 2.003 CPU sec, 2.078 real sec...
[M::process] read 714970 sequences (100000182 bp) in 1.580 CPU sec, 1.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304424, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.46, 84.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715030 reads in 169.255 CPU sec, 5.416 real sec
[M::mem_process_seqs] Processed 715020 reads in 94.323 CPU sec, 3.027 real sec
sorting block 38, #reads = 1092295 mem = 536878669 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304810, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (195.79, 85.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714834 sequences (100000170 bp) in 2.114 CPU sec, 2.156 real sec...
[M::process] read 714434 sequences (100000208 bp) in 1.420 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303835, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (199.97, 91.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715210 reads in 168.615 CPU sec, 5.376 real sec
[M::mem_process_seqs] Processed 714970 reads in 97.465 CPU sec, 3.130 real sec
sorting block 39, #reads = 1092501 mem = 536879598 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304474, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (203.82, 97.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714336 sequences (100000151 bp) in 2.511 CPU sec, 2.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303984, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (205.84, 99.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714212 sequences (100000176 bp) in 1.790 CPU sec, 1.790 real sec...
[M::mem_process_seqs] Processed 714834 reads in 170.195 CPU sec, 5.426 real sec
[M::mem_process_seqs] Processed 714434 reads in 97.180 CPU sec, 3.090 real sec
sorting block 40, #reads = 1092181 mem = 536878475 peak = 1177088000
sorting block 41, #reads = 1091700 mem = 536880250 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 303651, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (205.43, 98.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714240 sequences (100000258 bp) in 1.968 CPU sec, 2.001 real sec...
[M::process] read 714440 sequences (100000200 bp) in 1.982 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303557, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (209.46, 103.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 21, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218)
[M::mem_pestat] mean and std.dev: (177.09, 266.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1619)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 714336 reads in 165.437 CPU sec, 5.276 real sec
[M::mem_process_seqs] Processed 714212 reads in 95.345 CPU sec, 3.027 real sec
sorting block 42, #reads = 1091810 mem = 536879368 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 302827, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (209.52, 103.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716244 sequences (100000138 bp) in 2.407 CPU sec, 2.457 real sec...
[M::process] read 715604 sequences (100000283 bp) in 2.000 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303778, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (208.64, 102.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714240 reads in 172.980 CPU sec, 5.493 real sec
[M::mem_process_seqs] Processed 714440 reads in 99.920 CPU sec, 3.178 real sec
sorting block 43, #reads = 1090920 mem = 536878017 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303967, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 364)
[M::mem_pestat] mean and std.dev: (180.33, 66.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 81, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (88.11, 92.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715474 sequences (100000039 bp) in 1.910 CPU sec, 1.943 real sec...
[M::process] read 715500 sequences (100000039 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304544, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (187.81, 74.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716244 reads in 170.349 CPU sec, 5.448 real sec
[M::mem_process_seqs] Processed 715604 reads in 99.452 CPU sec, 3.190 real sec
sorting block 44, #reads = 1091607 mem = 536877431 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303990, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (189.32, 76.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1092274 mem = 536881386 peak = 1177088000
[M::process] read 715522 sequences (100000039 bp) in 2.691 CPU sec, 2.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303846, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.21, 79.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715474 reads in 174.099 CPU sec, 5.556 real sec
[M::mem_process_seqs] Processed 715500 reads in 102.305 CPU sec, 3.237 real sec
[M::process] read 715134 sequences (100000016 bp) in 2.204 CPU sec, 2.219 real sec...
sorting block 46, #reads = 1091815 mem = 536877712 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303639, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.25, 79.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715428 sequences (100000076 bp) in 2.240 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303718, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 715162 sequences (100000171 bp) in 1.850 CPU sec, 1.846 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.57, 85.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715522 reads in 170.047 CPU sec, 5.411 real sec
[M::mem_process_seqs] Processed 715134 reads in 97.058 CPU sec, 3.078 real sec
sorting block 47, #reads = 1091883 mem = 536877157 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303827, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (195.25, 85.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714984 sequences (100000189 bp) in 1.870 CPU sec, 1.945 real sec...
[M::process] read 714888 sequences (100000212 bp) in 1.390 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304117, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (197.08, 87.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715428 reads in 159.772 CPU sec, 5.122 real sec
[M::mem_process_seqs] Processed 715162 reads in 94.228 CPU sec, 3.014 real sec
sorting block 48, #reads = 1091734 mem = 536876986 peak = 1177088000
sorting block 49, #reads = 1091984 mem = 536879733 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304231, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (200.17, 91.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714808 sequences (100000078 bp) in 2.030 CPU sec, 2.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304203, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (202.39, 94.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714672 sequences (100000154 bp) in 1.990 CPU sec, 1.994 real sec...
[M::mem_process_seqs] Processed 714984 reads in 167.968 CPU sec, 5.362 real sec
[M::mem_process_seqs] Processed 714888 reads in 94.542 CPU sec, 2.999 real sec
sorting block 50, #reads = 1092013 mem = 536879039 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303687, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (202.89, 95.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714664 sequences (100000226 bp) in 2.496 CPU sec, 2.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304064, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (204.47, 97.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714574 sequences (100000265 bp) in 1.460 CPU sec, 1.455 real sec...
[M::mem_process_seqs] Processed 714808 reads in 162.785 CPU sec, 5.194 real sec
[M::mem_process_seqs] Processed 714672 reads in 94.348 CPU sec, 2.996 real sec
sorting block 51, #reads = 1091485 mem = 536879956 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303805, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (204.38, 97.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714500 sequences (100000166 bp) in 1.744 CPU sec, 1.797 real sec...
[M::process] read 714586 sequences (100000073 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303753, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (205.97, 99.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714664 reads in 162.471 CPU sec, 5.201 real sec
[M::mem_process_seqs] Processed 714574 reads in 90.664 CPU sec, 2.901 real sec
sorting block 52, #reads = 1091874 mem = 536878847 peak = 1177088000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303916, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (206.01, 99.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714388 sequences (100000171 bp) in 2.818 CPU sec, 2.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303800, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (205.53, 99.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714500 reads in 167.138 CPU sec, 5.311 real sec
[M::process] read 714250 sequences (100000145 bp) in 1.740 CPU sec, 1.748 real sec...
sorting block 53, #reads = 1091384 mem = 536879220 peak = 1177088000
[M::mem_process_seqs] Processed 714586 reads in 93.563 CPU sec, 2.967 real sec
sorting block 54, #reads = 1091420 mem = 536878925 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303959, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569)
[M::mem_pestat] mean and std.dev: (207.95, 102.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714310 sequences (100000217 bp) in 2.809 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303687, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (210.19, 104.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714388 reads in 161.088 CPU sec, 5.140 real sec
[M::mem_process_seqs] Processed 714250 reads in 95.948 CPU sec, 3.039 real sec
[M::process] read 714320 sequences (100000033 bp) in 2.838 CPU sec, 2.925 real sec...
sorting block 55, #reads = 1091449 mem = 536879658 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303225, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (209.23, 103.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714236 sequences (100000215 bp) in 1.951 CPU sec, 1.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303189, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (210.12, 104.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714106 sequences (100000072 bp) in 1.500 CPU sec, 1.499 real sec...
[M::mem_process_seqs] Processed 714310 reads in 168.075 CPU sec, 5.374 real sec
[M::mem_process_seqs] Processed 714320 reads in 98.110 CPU sec, 3.120 real sec
sorting block 56, #reads = 1091390 mem = 536880545 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303249, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (209.92, 104.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 725)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716048 sequences (100000110 bp) in 2.592 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 302939, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 165, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (211.28, 105.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714236 reads in 173.293 CPU sec, 5.519 real sec
[M::process] read 716392 sequences (100000096 bp) in 2.134 CPU sec, 2.151 real sec...
[M::mem_process_seqs] Processed 714106 reads in 99.937 CPU sec, 3.156 real sec
sorting block 57, #reads = 1090919 mem = 536878364 peak = 1205731328
sorting block 58, #reads = 1090727 mem = 536881452 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304089, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (187.12, 74.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716112 sequences (100000074 bp) in 1.803 CPU sec, 1.841 real sec...
[M::process] read 716058 sequences (100000156 bp) in 1.490 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304552, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 342)
[M::mem_pestat] mean and std.dev: (173.57, 57.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 407)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716048 reads in 167.289 CPU sec, 5.358 real sec
[M::mem_process_seqs] Processed 716392 reads in 94.897 CPU sec, 3.040 real sec
sorting block 59, #reads = 1091725 mem = 536878797 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304474, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (183.71, 70.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 464)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715788 sequences (100000170 bp) in 2.236 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304066, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (184.93, 71.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715566 sequences (100000178 bp) in 1.760 CPU sec, 1.758 real sec...
[M::mem_process_seqs] Processed 716112 reads in 165.551 CPU sec, 5.278 real sec
[M::mem_process_seqs] Processed 716058 reads in 95.457 CPU sec, 3.033 real sec
sorting block 60, #reads = 1092792 mem = 536880399 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304057, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (187.35, 74.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715496 sequences (100000240 bp) in 1.985 CPU sec, 2.028 real sec...
[M::process] read 715518 sequences (100000098 bp) in 1.500 CPU sec, 1.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304130, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (191.30, 79.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715788 reads in 169.195 CPU sec, 5.413 real sec
sorting block 61, #reads = 1091584 mem = 536879093 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303721, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (190.44, 78.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 552)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715320 sequences (100000030 bp) in 1.976 CPU sec, 1.980 real sec...
[M::mem_process_seqs] Processed 715496 reads in 94.790 CPU sec, 3.110 real sec
sorting block 62, #reads = 1092655 mem = 536876314 peak = 1205731328
[M::mem_process_seqs] Processed 715566 reads in 215.354 CPU sec, 6.962 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304200, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (191.86, 80.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715550 sequences (100000122 bp) in 1.875 CPU sec, 1.932 real sec...
[M::process] read 715484 sequences (100000081 bp) in 1.510 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303500, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460)
[M::mem_pestat] mean and std.dev: (191.58, 79.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715518 reads in 175.193 CPU sec, 5.584 real sec
sorting block 63, #reads = 1092166 mem = 536876983 peak = 1205731328
[M::mem_process_seqs] Processed 715320 reads in 98.116 CPU sec, 3.137 real sec
sorting block 64, #reads = 1092022 mem = 536879335 peak = 1205731328
[M::process] read 715888 sequences (100000244 bp) in 1.942 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303663, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (188.93, 76.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715654 sequences (100000245 bp) in 1.480 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304065, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (189.50, 76.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715550 reads in 165.005 CPU sec, 5.269 real sec
[M::mem_process_seqs] Processed 715484 reads in 96.327 CPU sec, 3.090 real sec
sorting block 65, #reads = 1091838 mem = 536878348 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303874, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (187.72, 74.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715970 sequences (100000186 bp) in 2.510 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304258, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (185.30, 71.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715888 reads in 173.615 CPU sec, 5.540 real sec
[M::mem_process_seqs] Processed 715654 reads in 104.852 CPU sec, 3.308 real sec
[M::process] read 715996 sequences (100000154 bp) in 2.114 CPU sec, 2.124 real sec...
sorting block 66, #reads = 1091984 mem = 536877649 peak = 1205731328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303895, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 352)
[M::mem_pestat] mean and std.dev: (177.48, 62.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 427)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715986 sequences (100000141 bp) in 2.353 CPU sec, 2.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304207, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (179.36, 64.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715970 reads in 166.755 CPU sec, 5.314 real sec
[M::mem_process_seqs] Processed 715996 reads in 96.520 CPU sec, 3.049 real sec
[M::process] read 715942 sequences (100000065 bp) in 2.143 CPU sec, 2.148 real sec...
sorting block 67, #reads = 1091482 mem = 536878400 peak = 1205731328
sorting block 68, #reads = 1091566 mem = 536879022 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304729, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (179.36, 64.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715464 sequences (100000049 bp) in 2.403 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304447, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 358)
[M::mem_pestat] mean and std.dev: (178.88, 64.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 436)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714950 sequences (100000050 bp) in 1.720 CPU sec, 1.719 real sec...
[M::mem_process_seqs] Processed 715986 reads in 163.246 CPU sec, 5.197 real sec
[M::mem_process_seqs] Processed 715942 reads in 92.526 CPU sec, 2.941 real sec
sorting block 69, #reads = 1092771 mem = 536879923 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304621, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (189.96, 77.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 398, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976)
[M::mem_pestat] mean and std.dev: (319.44, 351.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2626)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 714718 sequences (100000051 bp) in 2.151 CPU sec, 2.191 real sec...
[M::process] read 714626 sequences (100000049 bp) in 1.480 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 303509, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (198.74, 90.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715464 reads in 170.116 CPU sec, 5.431 real sec
[M::mem_process_seqs] Processed 714950 reads in 96.570 CPU sec, 3.091 real sec
sorting block 70, #reads = 1092497 mem = 536883796 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303313, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (202.80, 96.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714796 sequences (100000172 bp) in 2.748 CPU sec, 2.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303165, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (203.17, 96.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 27, 111)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279)
[M::mem_pestat] mean and std.dev: (37.75, 34.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 363)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 714718 reads in 177.828 CPU sec, 5.677 real sec
[M::mem_process_seqs] Processed 714626 reads in 103.491 CPU sec, 3.273 real sec
[M::process] read 715382 sequences (100000093 bp) in 2.497 CPU sec, 2.548 real sec...
sorting block 71, #reads = 1091632 mem = 536877078 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303134, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (204.97, 98.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1091289 mem = 536879673 peak = 1231499264
[M::process] read 716446 sequences (100000049 bp) in 2.337 CPU sec, 2.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303810, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (193.21, 83.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714796 reads in 179.008 CPU sec, 5.713 real sec
[M::mem_process_seqs] Processed 715382 reads in 103.767 CPU sec, 3.275 real sec
[M::process] read 716314 sequences (100000078 bp) in 2.263 CPU sec, 2.291 real sec...
sorting block 73, #reads = 1091416 mem = 536879925 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304258, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 345)
[M::mem_pestat] mean and std.dev: (175.00, 59.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716092 sequences (100000266 bp) in 2.303 CPU sec, 2.369 real sec...
[M::process] read 715848 sequences (100000123 bp) in 1.660 CPU sec, 1.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304490, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 364)
[M::mem_pestat] mean and std.dev: (180.38, 66.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 445)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716446 reads in 178.741 CPU sec, 5.709 real sec
[M::mem_process_seqs] Processed 716314 reads in 103.351 CPU sec, 3.284 real sec
sorting block 74, #reads = 1092783 mem = 536878782 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304230, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.66, 71.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (74, 112, 804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2264)
[M::mem_pestat] mean and std.dev: (424.40, 442.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2994)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 715784 sequences (100000190 bp) in 2.629 CPU sec, 2.821 real sec...
[M::process] read 715570 sequences (100000231 bp) in 1.479 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304162, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (186.47, 72.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716092 reads in 171.605 CPU sec, 5.473 real sec
[M::mem_process_seqs] Processed 715848 reads in 100.096 CPU sec, 3.185 real sec
sorting block 75, #reads = 1093056 mem = 536878327 peak = 1231499264
sorting block 76, #reads = 1092107 mem = 536881549 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304225, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (187.16, 73.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715372 sequences (100000133 bp) in 1.733 CPU sec, 1.774 real sec...
[M::process] read 715460 sequences (100000259 bp) in 1.780 CPU sec, 1.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304143, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (189.13, 76.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715784 reads in 158.933 CPU sec, 5.075 real sec
[M::mem_process_seqs] Processed 715570 reads in 96.472 CPU sec, 3.075 real sec
sorting block 77, #reads = 1092145 mem = 536880100 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304141, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (192.17, 80.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 572)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715398 sequences (100000283 bp) in 2.698 CPU sec, 2.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303799, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (190.72, 78.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715372 reads in 170.100 CPU sec, 5.407 real sec
[M::process] read 715394 sequences (100000088 bp) in 2.120 CPU sec, 2.124 real sec...
[M::mem_process_seqs] Processed 715460 reads in 97.149 CPU sec, 3.068 real sec
sorting block 78, #reads = 1092280 mem = 536878727 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303766, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (192.75, 81.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715334 sequences (100000193 bp) in 2.252 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304536, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (192.49, 81.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715658 sequences (100000006 bp) in 1.740 CPU sec, 1.741 real sec...
[M::mem_process_seqs] Processed 715398 reads in 160.724 CPU sec, 5.126 real sec
[M::mem_process_seqs] Processed 715394 reads in 90.611 CPU sec, 2.871 real sec
sorting block 79, #reads = 1092313 mem = 536879683 peak = 1231499264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 304006, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (193.65, 82.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715768 sequences (100000004 bp) in 2.468 CPU sec, 2.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304493, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::process] read 715636 sequences (100000087 bp) in 1.710 CPU sec, 1.703 real sec...
[M::mem_pestat] mean and std.dev: (188.17, 75.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715334 reads in 166.309 CPU sec, 5.299 real sec
sorting block 80, #reads = 1092274 mem = 536878972 peak = 1231499264
[M::mem_process_seqs] Processed 715658 reads in 92.469 CPU sec, 2.939 real sec
sorting block 81, #reads = 1092347 mem = 536877023 peak = 1240788992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303893, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (186.64, 73.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715492 sequences (100000047 bp) in 2.681 CPU sec, 2.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303771, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397)
[M::mem_pestat] mean and std.dev: (185.86, 72.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715768 reads in 173.620 CPU sec, 5.513 real sec
[M::process] read 715548 sequences (100000076 bp) in 2.010 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 715636 reads in 99.291 CPU sec, 3.145 real sec
sorting block 82, #reads = 1092811 mem = 536878450 peak = 1240788992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304313, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (187.36, 73.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715272 sequences (100000127 bp) in 2.448 CPU sec, 2.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304197, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (186.50, 73.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714890 sequences (100000214 bp) in 1.820 CPU sec, 1.822 real sec...
[M::mem_process_seqs] Processed 715492 reads in 170.283 CPU sec, 5.423 real sec
[M::mem_process_seqs] Processed 715548 reads in 96.988 CPU sec, 3.082 real sec
sorting block 83, #reads = 1091921 mem = 536879846 peak = 1240788992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303879, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (193.73, 83.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714964 sequences (100000229 bp) in 2.875 CPU sec, 3.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303932, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (202.47, 95.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715272 reads in 176.719 CPU sec, 5.615 real sec
[M::process] read 714680 sequences (100000257 bp) in 2.138 CPU sec, 2.152 real sec...
[M::mem_process_seqs] Processed 714890 reads in 102.543 CPU sec, 3.246 real sec
sorting block 84, #reads = 1092582 mem = 536878055 peak = 1240788992
sorting block 85, #reads = 1091970 mem = 536879569 peak = 1250791424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303324, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (201.21, 94.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714500 sequences (100000268 bp) in 2.001 CPU sec, 2.058 real sec...
[M::process] read 714878 sequences (100000112 bp) in 1.500 CPU sec, 1.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303778, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (203.55, 97.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714964 reads in 171.426 CPU sec, 5.482 real sec
[M::mem_process_seqs] Processed 714680 reads in 98.931 CPU sec, 3.154 real sec
sorting block 86, #reads = 1090158 mem = 536880387 peak = 1250791424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303616, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (204.55, 98.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716760 sequences (100000134 bp) in 2.520 CPU sec, 2.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303735, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517)
[M::mem_pestat] mean and std.dev: (198.02, 90.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (105, 163, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077)
[M::mem_pestat] mean and std.dev: (149.60, 112.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 716340 sequences (100000250 bp) in 1.720 CPU sec, 1.723 real sec...
[M::mem_process_seqs] Processed 714500 reads in 163.847 CPU sec, 5.213 real sec
[M::mem_process_seqs] Processed 714878 reads in 95.536 CPU sec, 3.035 real sec
sorting block 87, #reads = 1091384 mem = 536878164 peak = 1250791424
[M::process] read 716100 sequences (100000121 bp) in 2.371 CPU sec, 2.550 real sec...
[M::process] read 716024 sequences (100000025 bp) in 1.530 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304284, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 364)
[M::mem_pestat] mean and std.dev: (180.61, 66.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 445)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 79, 646)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908)
[M::mem_pestat] mean and std.dev: (355.86, 504.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2539)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 716340 reads in 88.862 CPU sec, 2.805 real sec
sorting block 88, #reads = 1091687 mem = 536878826 peak = 1250791424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304587, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 336)
[M::mem_pestat] mean and std.dev: (171.92, 55.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 399)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304370, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (184.25, 70.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716760 reads in 255.870 CPU sec, 8.155 real sec
[M::process] read 715932 sequences (100000092 bp) in 2.027 CPU sec, 2.042 real sec...
[M::mem_process_seqs] Processed 716100 reads in 109.633 CPU sec, 3.523 real sec
sorting block 89, #reads = 1092921 mem = 536877975 peak = 1250791424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304248, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (184.53, 71.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 469)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715776 sequences (100000222 bp) in 2.461 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304081, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (186.65, 73.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716024 reads in 171.601 CPU sec, 5.465 real sec
[M::process] read 715432 sequences (100000060 bp) in 1.949 CPU sec, 1.955 real sec...
sorting block 90, #reads = 1093048 mem = 536880373 peak = 1250791424
[M::mem_process_seqs] Processed 715932 reads in 99.948 CPU sec, 3.167 real sec
sorting block 91, #reads = 1092806 mem = 536880997 peak = 1250791424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304177, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (188.77, 76.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 532)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715358 sequences (100000204 bp) in 2.575 CPU sec, 2.734 real sec...
[M::process] read 715198 sequences (100000181 bp) in 1.660 CPU sec, 1.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303671, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (190.61, 78.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715776 reads in 173.913 CPU sec, 5.533 real sec
[M::mem_process_seqs] Processed 715432 reads in 101.880 CPU sec, 3.255 real sec
sorting block 92, #reads = 1092643 mem = 536878760 peak = 1250791424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303705, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493)
[M::mem_pestat] mean and std.dev: (195.91, 85.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715478 sequences (100000115 bp) in 2.118 CPU sec, 2.192 real sec...
[M::process] read 715132 sequences (100000245 bp) in 2.110 CPU sec, 2.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303914, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (194.15, 83.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715358 reads in 168.097 CPU sec, 5.345 real sec
[M::mem_process_seqs] Processed 715198 reads in 98.417 CPU sec, 3.130 real sec
sorting block 93, #reads = 1092226 mem = 536877236 peak = 1250791424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303894, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484)
[M::mem_pestat] mean and std.dev: (194.58, 84.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 596)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715038 sequences (100000060 bp) in 2.775 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 303810, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (195.32, 85.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715478 reads in 178.932 CPU sec, 5.703 real sec
[M::mem_process_seqs] Processed 715132 reads in 111.522 CPU sec, 3.534 real sec
[M::process] read 715052 sequences (100000146 bp) in 2.297 CPU sec, 2.309 real sec...
sorting block 94, #reads = 1092263 mem = 536878938 peak = 1250791424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303762, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.67, 87.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 95, #reads = 1091971 mem = 536878764 peak = 1254240256
[M::process] read 714902 sequences (100000195 bp) in 2.633 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303510, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.40, 87.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715038 reads in 194.707 CPU sec, 6.235 real sec
[M::process] read 715382 sequences (100000093 bp) in 2.141 CPU sec, 2.143 real sec...
[M::mem_process_seqs] Processed 715052 reads in 113.581 CPU sec, 3.610 real sec
sorting block 96, #reads = 1091755 mem = 536878034 peak = 1254240256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304380, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (197.96, 88.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 628)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715198 sequences (100000251 bp) in 2.712 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303928, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (192.69, 81.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714946 sequences (100000274 bp) in 2.080 CPU sec, 2.082 real sec...
[M::mem_process_seqs] Processed 714902 reads in 192.838 CPU sec, 6.175 real sec
[M::mem_process_seqs] Processed 715382 reads in 110.989 CPU sec, 3.535 real sec
sorting block 97, #reads = 1092118 mem = 536878407 peak = 1254240256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304315, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (193.04, 82.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714900 sequences (100000074 bp) in 2.630 CPU sec, 2.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303721, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (199.71, 91.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714590 sequences (100000020 bp) in 1.978 CPU sec, 1.980 real sec...
[M::mem_process_seqs] Processed 715198 reads in 195.775 CPU sec, 6.280 real sec
[M::mem_process_seqs] Processed 714946 reads in 113.572 CPU sec, 3.620 real sec
sorting block 98, #reads = 1092232 mem = 536879003 peak = 1256304640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303396, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (202.45, 95.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 99, #reads = 1091900 mem = 536878848 peak = 1258901504
[M::process] read 714682 sequences (100000041 bp) in 2.502 CPU sec, 2.604 real sec...
[M::process] read 714762 sequences (100000201 bp) in 2.094 CPU sec, 2.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303685, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (203.63, 97.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714900 reads in 199.082 CPU sec, 6.382 real sec
[M::mem_process_seqs] Processed 714590 reads in 113.735 CPU sec, 3.616 real sec
sorting block 100, #reads = 1091774 mem = 536880444 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303863, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (203.06, 96.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715014 sequences (100000109 bp) in 2.433 CPU sec, 2.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303662, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (203.28, 97.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 26, 45)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 129)
[M::mem_pestat] mean and std.dev: (20.60, 17.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 171)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 716330 sequences (100000001 bp) in 2.114 CPU sec, 2.120 real sec...
[M::mem_process_seqs] Processed 714682 reads in 196.000 CPU sec, 6.283 real sec
[M::mem_process_seqs] Processed 714762 reads in 112.058 CPU sec, 3.548 real sec
sorting block 101, #reads = 1090989 mem = 536879965 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304166, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (201.02, 95.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 716162 sequences (100000040 bp) in 2.883 CPU sec, 3.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304601, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 162, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 355)
[M::mem_pestat] mean and std.dev: (177.36, 62.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 428)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715014 reads in 182.329 CPU sec, 5.834 real sec
[M::mem_process_seqs] Processed 716330 reads in 106.625 CPU sec, 3.377 real sec
[M::process] read 716138 sequences (100000046 bp) in 2.211 CPU sec, 2.224 real sec...
sorting block 102, #reads = 1090747 mem = 536879586 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304483, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373)
[M::mem_pestat] mean and std.dev: (182.34, 68.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 456)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 103, #reads = 1092257 mem = 536880164 peak = 1258901504
[M::process] read 716050 sequences (100000221 bp) in 2.976 CPU sec, 3.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304538, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406)
[M::mem_pestat] mean and std.dev: (186.39, 73.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716162 reads in 184.775 CPU sec, 5.917 real sec
[M::mem_process_seqs] Processed 716138 reads in 104.089 CPU sec, 3.287 real sec
[M::process] read 715910 sequences (100000084 bp) in 2.736 CPU sec, 2.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304411, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (186.10, 72.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1092872 mem = 536878918 peak = 1258901504
[M::process] read 715744 sequences (100000270 bp) in 3.082 CPU sec, 3.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304497, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (188.07, 75.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 716050 reads in 180.359 CPU sec, 5.771 real sec
[M::mem_process_seqs] Processed 715910 reads in 104.085 CPU sec, 3.299 real sec
[M::process] read 715422 sequences (100000069 bp) in 2.571 CPU sec, 2.633 real sec...
sorting block 105, #reads = 1092710 mem = 536877985 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304040, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454)
[M::mem_pestat] mean and std.dev: (190.41, 78.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715474 sequences (100000132 bp) in 2.488 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303742, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (191.52, 80.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715744 reads in 189.693 CPU sec, 6.078 real sec
[M::mem_process_seqs] Processed 715422 reads in 109.586 CPU sec, 3.478 real sec
[M::process] read 715244 sequences (100000191 bp) in 2.051 CPU sec, 2.073 real sec...
sorting block 106, #reads = 1092248 mem = 536879970 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303980, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 163, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (194.76, 84.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715348 sequences (100000260 bp) in 3.164 CPU sec, 3.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303852, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.55, 88.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715474 reads in 183.404 CPU sec, 5.912 real sec
sorting block 107, #reads = 1092149 mem = 536879305 peak = 1258901504
[M::mem_process_seqs] Processed 715244 reads in 105.969 CPU sec, 3.370 real sec
[M::process] read 715050 sequences (100000135 bp) in 2.705 CPU sec, 2.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 304109, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (196.20, 86.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 108, #reads = 1092018 mem = 536882721 peak = 1258901504
[M::process] read 714914 sequences (100000132 bp) in 2.245 CPU sec, 2.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303766, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (197.26, 88.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 624)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715348 reads in 185.034 CPU sec, 5.956 real sec
[M::mem_process_seqs] Processed 715050 reads in 106.542 CPU sec, 3.390 real sec
[M::process] read 714998 sequences (100000179 bp) in 1.630 CPU sec, 1.653 real sec...
sorting block 109, #reads = 1091894 mem = 536877338 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303942, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (199.81, 91.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714996 sequences (100000222 bp) in 2.700 CPU sec, 2.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303593, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (201.07, 93.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714914 reads in 183.713 CPU sec, 5.868 real sec
[M::mem_process_seqs] Processed 714998 reads in 106.677 CPU sec, 3.362 real sec
[M::process] read 715116 sequences (100000161 bp) in 2.192 CPU sec, 2.203 real sec...
sorting block 110, #reads = 1091705 mem = 536877284 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303501, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (202.08, 94.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 715012 sequences (100000194 bp) in 2.502 CPU sec, 2.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 304063, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (200.78, 93.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714786 sequences (100000033 bp) in 1.993 CPU sec, 1.993 real sec...
[M::mem_process_seqs] Processed 714996 reads in 183.163 CPU sec, 5.851 real sec
sorting block 111, #reads = 1091356 mem = 536878275 peak = 1258901504
[M::mem_process_seqs] Processed 715116 reads in 103.743 CPU sec, 3.276 real sec
sorting block 112, #reads = 1091724 mem = 536879029 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303685, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529)
[M::mem_pestat] mean and std.dev: (200.55, 93.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714646 sequences (100000244 bp) in 2.429 CPU sec, 2.540 real sec...
[M::process] read 714568 sequences (100000240 bp) in 1.889 CPU sec, 1.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 303731, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (204.03, 97.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 715012 reads in 187.041 CPU sec, 5.984 real sec
[M::mem_process_seqs] Processed 714786 reads in 107.374 CPU sec, 3.402 real sec
sorting block 113, #reads = 1091813 mem = 536878979 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303863, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (204.90, 98.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714474 sequences (100000189 bp) in 2.588 CPU sec, 2.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303447, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (204.75, 98.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714458 sequences (100000257 bp) in 1.908 CPU sec, 1.911 real sec...
[M::mem_process_seqs] Processed 714646 reads in 184.315 CPU sec, 5.893 real sec
[M::mem_process_seqs] Processed 714568 reads in 101.508 CPU sec, 3.197 real sec
sorting block 114, #reads = 1091509 mem = 536879613 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 302955, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (205.87, 100.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 693)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 714668 sequences (100000144 bp) in 2.898 CPU sec, 3.103 real sec...
[M::process] read 343112 sequences (47997460 bp) in 1.171 CPU sec, 1.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303421, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (205.33, 99.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714474 reads in 185.105 CPU sec, 5.952 real sec
[M::mem_process_seqs] Processed 714458 reads in 105.839 CPU sec, 3.363 real sec
sorting block 115, #reads = 1091678 mem = 536878653 peak = 1258901504
sorting block 116, #reads = 1091290 mem = 536882561 peak = 1258901504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 303657, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 164, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (204.65, 99.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 145544, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 164, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (201.24, 95.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 714668 reads in 136.278 CPU sec, 4.476 real sec
[M::mem_process_seqs] Processed 343112 reads in 54.673 CPU sec, 1.779 real sec
sorting block 117, #reads = 1091093 mem = 536879289 peak = 1258901504
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000606a6
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef 40417f5e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 128379262
overall: 69921406976 mem 17567.788 user 486.365 sys 593.468 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260529_LH00995_0049_B23YT2HLT4.D0001483_22q.L007\tPU:L007\tSM:D0001483_22q\tLB:D0001483_22q\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.D0001483_22q_R1.fastq.gz 0003.D0001483_22q_R2.fastq.gz
[main] Real time: 593.507 sec; CPU: 18054.157 sec
sorting block 118, #reads = 178982 mem = 88017703 peak = 1258901504
uniq header count 1
merging 119 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000606a6
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef 40417f5e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz
threads: 31 max 128
algo: util-sort
license: sentieon:util=1
output file size: 5350673630
output reads: 129034702
bam_mem_sort: 119 calls 110.839 user 1.440 sys 112.458 real
bam_write: 119 calls 42.277 user 1.772 sys 48.589 real
execute: 1 calls 34.886 user 1.216 sys 631.638 real
merge_files: 1 calls 34.859 user 1.192 sys 38.013 real
parse_chunk: 1891 calls 115.451 user 22.577 sys 140.017 real
read_chunk: 5116 calls 56.233 user 31.209 sys 553.774 real
sort_block: 1 calls 37.372 user 30.783 sys 530.513 real
write_chunk: 3223 calls 34.428 user 0.923 sys 36.914 real
overall: 2779148288 mem 1603.691 user 76.230 sys 631.656 real
ls: cannot access '*.cram': No such file or directory