cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1877.828 CPU sec, 64.934 real sec
[M::process] read 677038 sequences (100000277 bp) in 0.885 CPU sec, 0.889 real sec...
[M::process] read 676658 sequences (100000038 bp) in 1.398 CPU sec, 1.461 real sec...
[M::process] read 676654 sequences (100000209 bp) in 1.371 CPU sec, 1.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287852, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.28, 108.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676944 sequences (100000020 bp) in 1.359 CPU sec, 1.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287861, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.34, 112.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287968, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.99, 113.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677038 reads in 273.237 CPU sec, 8.849 real sec
[M::mem_process_seqs] Processed 676658 reads in 179.965 CPU sec, 5.903 real sec
[M::mem_process_seqs] Processed 676654 reads in 187.942 CPU sec, 6.193 real sec
sorting block 0, #reads = 1081818 mem = 536879745 peak = 644173824
[M::process] read 676846 sequences (100000054 bp) in 1.558 CPU sec, 1.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288123, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.53, 108.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676922 sequences (100000244 bp) in 1.361 CPU sec, 1.392 real sec...
[M::process] read 676878 sequences (100000277 bp) in 1.411 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288003, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.20, 108.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676944 reads in 186.574 CPU sec, 5.966 real sec
sorting block 1, #reads = 1082127 mem = 536877901 peak = 944590848
[M::mem_process_seqs] Processed 676846 reads in 105.567 CPU sec, 3.383 real sec
sorting block 2, #reads = 1082326 mem = 536878457 peak = 955736064
[M::process] read 676656 sequences (100000015 bp) in 2.044 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288739, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.12, 108.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676852 sequences (100000011 bp) in 1.411 CPU sec, 1.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287846, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.75, 110.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287967, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.15, 111.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 119, 1250)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3718)
[M::mem_pestat] mean and std.dev: (365.00, 601.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4952)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676922 reads in 272.882 CPU sec, 8.805 real sec
[M::mem_process_seqs] Processed 676878 reads in 190.023 CPU sec, 6.150 real sec
[M::mem_process_seqs] Processed 676656 reads in 198.888 CPU sec, 6.473 real sec
sorting block 3, #reads = 1082789 mem = 536878315 peak = 955736064
[M::process] read 675978 sequences (100000177 bp) in 1.556 CPU sec, 1.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288058, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.28, 111.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1081912 mem = 536877258 peak = 955736064
[M::process] read 676164 sequences (100000116 bp) in 1.838 CPU sec, 2.141 real sec...
[M::process] read 676130 sequences (100000095 bp) in 1.340 CPU sec, 1.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287544, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.88, 123.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 10, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1952)
[M::mem_pestat] mean and std.dev: (319.40, 552.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2600)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676852 reads in 192.219 CPU sec, 6.219 real sec
[M::mem_process_seqs] Processed 675978 reads in 108.566 CPU sec, 3.478 real sec
sorting block 5, #reads = 1081969 mem = 536877635 peak = 955736064
[M::process] read 676418 sequences (100000206 bp) in 2.150 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287456, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.60, 120.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676862 sequences (100000065 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287742, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.02, 122.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287939, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.35, 115.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676164 reads in 276.280 CPU sec, 8.922 real sec
[M::mem_process_seqs] Processed 676130 reads in 192.704 CPU sec, 6.257 real sec
[M::mem_process_seqs] Processed 676418 reads in 201.318 CPU sec, 6.557 real sec
sorting block 6, #reads = 1081862 mem = 536882650 peak = 955736064
[M::process] read 676780 sequences (100000000 bp) in 2.052 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287930, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.87, 111.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1081571 mem = 536879790 peak = 955736064
[M::process] read 676748 sequences (100000109 bp) in 1.856 CPU sec, 1.947 real sec...
[M::process] read 676970 sequences (100000183 bp) in 1.245 CPU sec, 1.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288599, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.27, 113.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676862 reads in 188.823 CPU sec, 6.099 real sec
[M::mem_process_seqs] Processed 676780 reads in 104.501 CPU sec, 3.341 real sec
sorting block 8, #reads = 1082486 mem = 536879626 peak = 955736064
[M::process] read 676884 sequences (100000194 bp) in 1.959 CPU sec, 2.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288180, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.03, 112.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677064 sequences (100000220 bp) in 1.475 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287888, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.68, 108.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288116, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.83, 110.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676748 reads in 277.652 CPU sec, 8.932 real sec
[M::mem_process_seqs] Processed 676970 reads in 195.654 CPU sec, 6.306 real sec
[M::mem_process_seqs] Processed 676884 reads in 204.109 CPU sec, 6.612 real sec
sorting block 9, #reads = 1082353 mem = 536876889 peak = 955736064
[M::process] read 676790 sequences (100000273 bp) in 1.895 CPU sec, 2.057 real sec...
sorting block 10, #reads = 1082502 mem = 536879438 peak = 955736064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288290, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.24, 109.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676776 sequences (100000116 bp) in 1.812 CPU sec, 1.981 real sec...
[M::process] read 676344 sequences (100000119 bp) in 1.399 CPU sec, 1.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288391, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.16, 111.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677064 reads in 191.702 CPU sec, 6.205 real sec
[M::mem_process_seqs] Processed 676790 reads in 108.653 CPU sec, 3.496 real sec
sorting block 11, #reads = 1082574 mem = 536877536 peak = 955736064
[M::process] read 676340 sequences (100000073 bp) in 2.173 CPU sec, 2.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287771, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.73, 112.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676144 sequences (100000038 bp) in 1.431 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287435, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.58, 119.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676776 reads in 189.068 CPU sec, 6.110 real sec
[M::mem_process_seqs] Processed 676344 reads in 104.094 CPU sec, 3.423 real sec
sorting block 12, #reads = 1082536 mem = 536880751 peak = 955736064
[M::process] read 676254 sequences (100000122 bp) in 1.754 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287791, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.85, 119.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676928 sequences (100000074 bp) in 1.402 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287162, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.08, 123.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676340 reads in 190.712 CPU sec, 6.159 real sec
[M::mem_process_seqs] Processed 676144 reads in 107.192 CPU sec, 3.440 real sec
sorting block 13, #reads = 1081977 mem = 536879078 peak = 967213056
sorting block 14, #reads = 1082049 mem = 536878991 peak = 967213056
[M::process] read 676812 sequences (100000070 bp) in 1.741 CPU sec, 1.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288014, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.01, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 26, 95)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279)
[M::mem_pestat] mean and std.dev: (32.00, 43.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 371)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676684 sequences (100000187 bp) in 1.439 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287848, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.96, 114.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288665, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.15, 110.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676254 reads in 265.663 CPU sec, 8.563 real sec
[M::mem_process_seqs] Processed 676812 reads in 184.833 CPU sec, 5.956 real sec
[M::mem_process_seqs] Processed 676928 reads in 193.194 CPU sec, 6.252 real sec
sorting block 15, #reads = 1081739 mem = 536878640 peak = 967213056
[M::process] read 676824 sequences (100000270 bp) in 2.050 CPU sec, 2.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288704, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.32, 114.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 215, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (224.67, 187.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 16, #reads = 1082011 mem = 536878638 peak = 978894848
[M::process] read 676886 sequences (100000227 bp) in 1.922 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288835, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.68, 112.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 26, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286)
[M::mem_pestat] mean and std.dev: (26.88, 29.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 378)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676724 sequences (100000041 bp) in 1.474 CPU sec, 1.507 real sec...
[M::mem_process_seqs] Processed 676684 reads in 184.162 CPU sec, 5.983 real sec
[M::mem_process_seqs] Processed 676824 reads in 105.304 CPU sec, 3.390 real sec
sorting block 17, #reads = 1082920 mem = 536879885 peak = 978894848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288552, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.44, 110.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676852 sequences (100000183 bp) in 2.170 CPU sec, 2.322 real sec...
[M::process] read 676724 sequences (100000083 bp) in 1.448 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288290, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.49, 111.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 334, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1843)
[M::mem_pestat] mean and std.dev: (319.11, 259.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2427)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676886 reads in 179.674 CPU sec, 5.798 real sec
[M::mem_process_seqs] Processed 676724 reads in 100.811 CPU sec, 3.255 real sec
sorting block 18, #reads = 1082550 mem = 536877493 peak = 978894848
[M::process] read 676696 sequences (100000054 bp) in 1.754 CPU sec, 1.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288280, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.04, 111.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676592 sequences (100000149 bp) in 1.501 CPU sec, 1.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288282, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.89, 113.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288535, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.13, 115.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676852 reads in 267.390 CPU sec, 8.603 real sec
[M::mem_process_seqs] Processed 676724 reads in 186.945 CPU sec, 6.013 real sec
[M::mem_process_seqs] Processed 676696 reads in 195.363 CPU sec, 6.317 real sec
sorting block 19, #reads = 1082316 mem = 536878361 peak = 978894848
[M::process] read 676354 sequences (100000168 bp) in 1.892 CPU sec, 2.187 real sec...
sorting block 20, #reads = 1082661 mem = 536879484 peak = 988590080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288197, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.18, 117.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 417, 1766)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5274)
[M::mem_pestat] mean and std.dev: (750.29, 783.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7028)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676360 sequences (100000194 bp) in 2.070 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288106, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.62, 118.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676528 sequences (100000004 bp) in 1.488 CPU sec, 1.498 real sec...
[M::mem_process_seqs] Processed 676592 reads in 187.616 CPU sec, 6.117 real sec
sorting block 21, #reads = 1082416 mem = 536882907 peak = 988590080
[M::process] read 676782 sequences (100000188 bp) in 1.391 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287951, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.37, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676360 reads in 95.506 CPU sec, 3.148 real sec
[M::mem_process_seqs] Processed 676354 reads in 232.320 CPU sec, 7.514 real sec
sorting block 22, #reads = 1082008 mem = 536876069 peak = 988590080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287964, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.38, 114.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676654 sequences (100000151 bp) in 1.908 CPU sec, 2.146 real sec...
[M::process] read 676382 sequences (100000193 bp) in 1.495 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288120, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.62, 111.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676528 reads in 189.354 CPU sec, 6.088 real sec
[M::mem_process_seqs] Processed 676782 reads in 103.573 CPU sec, 3.322 real sec
sorting block 23, #reads = 1081592 mem = 536879520 peak = 988590080
[M::process] read 676818 sequences (100000085 bp) in 1.731 CPU sec, 1.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287640, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.12, 113.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676972 sequences (100000125 bp) in 1.338 CPU sec, 1.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287907, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.34, 116.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676654 reads in 182.681 CPU sec, 5.927 real sec
[M::mem_process_seqs] Processed 676382 reads in 102.891 CPU sec, 3.329 real sec
sorting block 24, #reads = 1081578 mem = 536880205 peak = 988590080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288023, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.73, 109.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676742 sequences (100000046 bp) in 1.866 CPU sec, 2.080 real sec...
[M::process] read 676610 sequences (100000056 bp) in 1.486 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288103, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.25, 109.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676818 reads in 182.057 CPU sec, 5.854 real sec
[M::mem_process_seqs] Processed 676972 reads in 101.873 CPU sec, 3.244 real sec
sorting block 25, #reads = 1082288 mem = 536879435 peak = 988590080
sorting block 26, #reads = 1082305 mem = 536880222 peak = 1001885696
[M::process] read 676744 sequences (100000182 bp) in 1.743 CPU sec, 1.901 real sec...
[M::process] read 676562 sequences (100000030 bp) in 1.453 CPU sec, 1.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287906, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.53, 112.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676610 reads in 95.741 CPU sec, 3.082 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288174, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.64, 111.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676374 sequences (100000202 bp) in 1.706 CPU sec, 1.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288218, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.57, 112.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676742 reads in 272.537 CPU sec, 8.750 real sec
[M::mem_process_seqs] Processed 676744 reads in 197.960 CPU sec, 6.360 real sec
sorting block 27, #reads = 1082150 mem = 536878778 peak = 1001885696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288190, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.13, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676264 sequences (100000172 bp) in 1.893 CPU sec, 2.183 real sec...
[M::process] read 676080 sequences (100000019 bp) in 1.436 CPU sec, 1.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287754, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.10, 117.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676562 reads in 181.640 CPU sec, 5.850 real sec
sorting block 28, #reads = 1082593 mem = 536877635 peak = 1017643008
[M::mem_process_seqs] Processed 676374 reads in 102.000 CPU sec, 3.284 real sec
sorting block 29, #reads = 1082550 mem = 536878606 peak = 1017643008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288684, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.76, 117.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 952, 952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2714)
[M::mem_pestat] mean and std.dev: (685.38, 577.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3595)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676906 sequences (100000103 bp) in 1.947 CPU sec, 2.094 real sec...
[M::process] read 676776 sequences (100000277 bp) in 1.504 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287668, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.79, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 61, 63)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 113)
[M::mem_pestat] mean and std.dev: (46.89, 17.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 138)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676264 reads in 177.612 CPU sec, 5.698 real sec
[M::mem_process_seqs] Processed 676080 reads in 98.894 CPU sec, 3.153 real sec
sorting block 30, #reads = 1081589 mem = 536878540 peak = 1017643008
[M::process] read 676914 sequences (100000211 bp) in 1.823 CPU sec, 1.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287680, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.30, 111.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677234 sequences (100000127 bp) in 1.461 CPU sec, 1.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287071, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.39, 112.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 73, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246)
[M::mem_pestat] mean and std.dev: (35.62, 34.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 323)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287818, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.84, 112.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676906 reads in 281.306 CPU sec, 9.042 real sec
[M::mem_process_seqs] Processed 676776 reads in 196.854 CPU sec, 6.325 real sec
[M::mem_process_seqs] Processed 676914 reads in 205.144 CPU sec, 6.612 real sec
sorting block 31, #reads = 1081492 mem = 536878040 peak = 1017643008
[M::process] read 677044 sequences (100000118 bp) in 1.864 CPU sec, 2.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288105, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.26, 107.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1082052 mem = 536881349 peak = 1017643008
[M::process] read 677174 sequences (100000247 bp) in 1.790 CPU sec, 2.034 real sec...
[M::process] read 677030 sequences (100000179 bp) in 1.455 CPU sec, 1.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287658, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.89, 110.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677234 reads in 194.862 CPU sec, 6.307 real sec
[M::mem_process_seqs] Processed 677044 reads in 108.800 CPU sec, 3.466 real sec
sorting block 33, #reads = 1082219 mem = 536878505 peak = 1017643008
[M::process] read 676954 sequences (100000295 bp) in 1.950 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287606, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.41, 107.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 154, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1495)
[M::mem_pestat] mean and std.dev: (326.10, 318.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1969)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676982 sequences (100000168 bp) in 1.462 CPU sec, 1.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287849, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.23, 108.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288127, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.72, 108.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677174 reads in 282.330 CPU sec, 9.087 real sec
[M::mem_process_seqs] Processed 677030 reads in 197.369 CPU sec, 6.351 real sec
[M::mem_process_seqs] Processed 676954 reads in 205.642 CPU sec, 6.638 real sec
sorting block 34, #reads = 1082339 mem = 536879724 peak = 1017643008
sorting block 35, #reads = 1082896 mem = 536879231 peak = 1048748032
[M::process] read 676294 sequences (100000280 bp) in 2.302 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287512, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.45, 111.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 419, 9646)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28920)
[M::mem_pestat] mean and std.dev: (3334.33, 4466.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38557)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676542 sequences (100000226 bp) in 2.018 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287091, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.43, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676302 sequences (100000107 bp) in 1.563 CPU sec, 1.573 real sec...
[M::mem_process_seqs] Processed 676982 reads in 199.325 CPU sec, 6.409 real sec
[M::mem_process_seqs] Processed 676294 reads in 112.418 CPU sec, 3.584 real sec
sorting block 36, #reads = 1082500 mem = 536878559 peak = 1048748032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287814, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.09, 116.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676946 sequences (100000230 bp) in 1.931 CPU sec, 2.117 real sec...
[M::process] read 676890 sequences (100000281 bp) in 1.551 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287213, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.31, 120.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676542 reads in 185.759 CPU sec, 5.990 real sec
[M::mem_process_seqs] Processed 676302 reads in 105.820 CPU sec, 3.380 real sec
sorting block 37, #reads = 1082124 mem = 536879603 peak = 1048748032
sorting block 38, #reads = 1081324 mem = 536877287 peak = 1060315136
[M::process] read 676862 sequences (100000294 bp) in 1.848 CPU sec, 2.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287785, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.46, 110.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677048 sequences (100000017 bp) in 1.472 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287521, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.29, 110.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676946 reads in 181.070 CPU sec, 5.854 real sec
[M::mem_process_seqs] Processed 676890 reads in 102.314 CPU sec, 3.305 real sec
sorting block 39, #reads = 1082132 mem = 536879127 peak = 1060315136
[M::process] read 677146 sequences (100000207 bp) in 2.050 CPU sec, 2.182 real sec...
[M::process] read 677136 sequences (100000005 bp) in 1.557 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288194, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.07, 106.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 13, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199)
[M::mem_pestat] mean and std.dev: (35.36, 47.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 263)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677048 reads in 93.969 CPU sec, 2.991 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288156, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.72, 109.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 206, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1874)
[M::mem_pestat] mean and std.dev: (407.91, 391.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2469)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677118 sequences (100000063 bp) in 1.525 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288224, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.78, 106.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676862 reads in 275.704 CPU sec, 8.824 real sec
sorting block 40, #reads = 1082263 mem = 536879133 peak = 1060315136
[M::mem_process_seqs] Processed 677146 reads in 111.848 CPU sec, 3.595 real sec
sorting block 41, #reads = 1082488 mem = 536876509 peak = 1073016832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288140, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.16, 108.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677030 sequences (100000002 bp) in 2.118 CPU sec, 2.277 real sec...
[M::process] read 677134 sequences (100000230 bp) in 1.502 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288000, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.86, 107.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677136 reads in 178.515 CPU sec, 5.730 real sec
[M::mem_process_seqs] Processed 677118 reads in 100.858 CPU sec, 3.234 real sec
sorting block 42, #reads = 1082503 mem = 536879023 peak = 1073016832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287586, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.25, 108.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676684 sequences (100000211 bp) in 2.127 CPU sec, 2.336 real sec...
[M::process] read 676802 sequences (100000035 bp) in 1.619 CPU sec, 1.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287542, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.50, 109.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677030 reads in 184.121 CPU sec, 5.910 real sec
[M::mem_process_seqs] Processed 677134 reads in 104.147 CPU sec, 3.328 real sec
sorting block 43, #reads = 1082203 mem = 536878988 peak = 1073016832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287348, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.10, 118.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676578 sequences (100000066 bp) in 2.017 CPU sec, 2.270 real sec...
[M::process] read 677046 sequences (100000017 bp) in 1.394 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287696, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.15, 113.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676684 reads in 188.280 CPU sec, 6.081 real sec
[M::mem_process_seqs] Processed 676802 reads in 105.169 CPU sec, 3.363 real sec
sorting block 44, #reads = 1082775 mem = 536879177 peak = 1073016832
sorting block 45, #reads = 1082537 mem = 536879039 peak = 1091182592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287766, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.98, 116.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677044 sequences (100000205 bp) in 2.124 CPU sec, 2.310 real sec...
[M::process] read 676642 sequences (100000266 bp) in 1.486 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288024, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.24, 110.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676578 reads in 180.162 CPU sec, 5.783 real sec
[M::mem_process_seqs] Processed 677046 reads in 98.477 CPU sec, 3.148 real sec
sorting block 46, #reads = 1082196 mem = 536879124 peak = 1091182592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288180, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.42, 111.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 416, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499)
[M::mem_pestat] mean and std.dev: (315.22, 232.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1921)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676734 sequences (100000190 bp) in 2.047 CPU sec, 2.207 real sec...
[M::process] read 677176 sequences (100000026 bp) in 1.499 CPU sec, 1.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287363, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.98, 113.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677044 reads in 181.127 CPU sec, 5.819 real sec
[M::process] read 677030 sequences (100000291 bp) in 1.697 CPU sec, 1.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287835, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.38, 111.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 22, 27)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 77)
[M::mem_pestat] mean and std.dev: (14.22, 12.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 102)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676734 reads in 96.204 CPU sec, 3.065 real sec
sorting block 47, #reads = 1082478 mem = 536879606 peak = 1091182592
[M::mem_process_seqs] Processed 676642 reads in 233.420 CPU sec, 7.475 real sec
sorting block 48, #reads = 1082037 mem = 536877279 peak = 1095569408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288171, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.16, 105.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677048 sequences (100000111 bp) in 2.087 CPU sec, 2.233 real sec...
[M::process] read 676964 sequences (100000122 bp) in 1.510 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288415, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.03, 106.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677176 reads in 189.496 CPU sec, 6.053 real sec
[M::mem_process_seqs] Processed 677030 reads in 102.389 CPU sec, 3.277 real sec
sorting block 49, #reads = 1082460 mem = 536879551 peak = 1095569408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287953, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.43, 108.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676902 sequences (100000290 bp) in 2.009 CPU sec, 2.209 real sec...
[M::process] read 676816 sequences (100000111 bp) in 1.513 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288063, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.11, 109.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677048 reads in 184.593 CPU sec, 5.928 real sec
[M::mem_process_seqs] Processed 676964 reads in 103.553 CPU sec, 3.293 real sec
sorting block 50, #reads = 1082773 mem = 536878759 peak = 1095569408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288025, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.49, 110.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676808 sequences (100000139 bp) in 1.932 CPU sec, 2.136 real sec...
[M::process] read 676794 sequences (100000081 bp) in 1.484 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287914, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.13, 111.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676902 reads in 181.876 CPU sec, 5.849 real sec
[M::mem_process_seqs] Processed 676816 reads in 103.367 CPU sec, 3.305 real sec
sorting block 51, #reads = 1082792 mem = 536879119 peak = 1095569408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287684, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.40, 114.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676786 sequences (100000073 bp) in 2.118 CPU sec, 2.222 real sec...
[M::process] read 677278 sequences (100000130 bp) in 1.568 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288256, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.33, 113.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676808 reads in 184.424 CPU sec, 5.900 real sec
sorting block 52, #reads = 1082178 mem = 536879451 peak = 1095569408
[M::mem_process_seqs] Processed 676794 reads in 101.969 CPU sec, 3.247 real sec
sorting block 53, #reads = 1082688 mem = 536877495 peak = 1109864448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288346, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.97, 113.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677192 sequences (100000014 bp) in 2.006 CPU sec, 2.371 real sec...
[M::process] read 676852 sequences (100000204 bp) in 1.520 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288235, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.43, 107.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 80, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253)
[M::mem_pestat] mean and std.dev: (44.78, 39.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 334)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676786 reads in 177.176 CPU sec, 5.743 real sec
[M::mem_process_seqs] Processed 677278 reads in 101.206 CPU sec, 3.253 real sec
sorting block 54, #reads = 1082507 mem = 536879042 peak = 1109864448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288365, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (230.48, 113.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677002 sequences (100000140 bp) in 2.057 CPU sec, 2.153 real sec...
[M::process] read 677202 sequences (100000012 bp) in 1.560 CPU sec, 1.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287984, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.44, 115.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677192 reads in 179.520 CPU sec, 5.767 real sec
[M::mem_process_seqs] Processed 676852 reads in 101.217 CPU sec, 3.278 real sec
sorting block 55, #reads = 1082393 mem = 536880681 peak = 1109864448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288216, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.80, 109.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677162 sequences (100000287 bp) in 2.064 CPU sec, 2.282 real sec...
[M::process] read 676934 sequences (100000174 bp) in 1.499 CPU sec, 1.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288353, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.47, 101.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677002 reads in 179.944 CPU sec, 5.779 real sec
[M::mem_process_seqs] Processed 677202 reads in 101.239 CPU sec, 3.218 real sec
sorting block 56, #reads = 1082228 mem = 536876144 peak = 1109864448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288381, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (222.74, 103.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676902 sequences (100000236 bp) in 2.098 CPU sec, 2.195 real sec...
[M::process] read 676870 sequences (100000013 bp) in 1.584 CPU sec, 1.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288085, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.58, 109.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677162 reads in 178.691 CPU sec, 5.733 real sec
sorting block 57, #reads = 1082098 mem = 536879397 peak = 1109864448
[M::mem_process_seqs] Processed 676934 reads in 100.933 CPU sec, 3.233 real sec
sorting block 58, #reads = 1082699 mem = 536878684 peak = 1129648128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288392, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.00, 111.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676776 sequences (100000088 bp) in 2.094 CPU sec, 2.286 real sec...
[M::process] read 676678 sequences (100000109 bp) in 1.561 CPU sec, 1.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288076, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.73, 112.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676902 reads in 182.233 CPU sec, 5.862 real sec
[M::mem_process_seqs] Processed 676870 reads in 103.304 CPU sec, 3.356 real sec
sorting block 59, #reads = 1082527 mem = 536881349 peak = 1129648128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288123, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.26, 114.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676582 sequences (100000076 bp) in 1.780 CPU sec, 2.110 real sec...
[M::process] read 185056 sequences (27351456 bp) in 0.617 CPU sec, 0.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288295, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.74, 115.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676776 reads in 179.371 CPU sec, 5.814 real sec
[M::mem_process_seqs] Processed 676678 reads in 101.156 CPU sec, 3.254 real sec
sorting block 60, #reads = 1082195 mem = 536880585 peak = 1129648128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287722, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.58, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 78774, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.36, 117.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676582 reads in 115.777 CPU sec, 3.784 real sec
[M::mem_process_seqs] Processed 185056 reads in 32.602 CPU sec, 1.060 real sec
sorting block 61, #reads = 1082023 mem = 536877071 peak = 1133318144
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67185844
overall: 69732028416 mem 11039.027 user 397.199 sys 385.637 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L005\tPU:L005\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T21-D0003376_R1.fastq.gz 0001.T21-D0003376_R2.fastq.gz
[main] Real time: 385.675 sec; CPU: 11436.230 sec
sorting block 62, #reads = 605714 mem = 300553456 peak = 1133318144
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2621338777
output reads: 67705950
bam_mem_sort: 63 calls 36.384 user 1.229 sys 38.521 real
bam_write: 63 calls 33.846 user 1.985 sys 61.429 real
execute: 1 calls 18.119 user 1.659 sys 411.801 real
merge_files: 1 calls 18.096 user 1.639 sys 25.661 real
parse_chunk: 1001 calls 46.007 user 10.095 sys 65.309 real
read_chunk: 2696 calls 20.509 user 12.092 sys 322.195 real
sort_block: 1 calls 13.895 user 11.641 sys 311.436 real
write_chunk: 1693 calls 17.737 user 1.435 sys 24.929 real
overall: 2441347072 mem 693.602 user 39.868 sys 411.821 real
ls: cannot access '*.cram': No such file or directory