This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1882.996 CPU sec, 71.047 real sec
[M::process] read 676962 sequences (100000070 bp) in 1.324 CPU sec, 1.328 real sec...
[M::process] read 676800 sequences (100000288 bp) in 1.819 CPU sec, 1.861 real sec...
[M::process] read 676600 sequences (100000260 bp) in 1.694 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288709, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.74, 109.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677112 sequences (100000250 bp) in 1.688 CPU sec, 1.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288509, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.29, 112.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288395, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.69, 112.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 13, 39)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 91)
[M::mem_pestat] mean and std.dev: (24.40, 21.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 117)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676962 reads in 316.722 CPU sec, 10.410 real sec
[M::mem_process_seqs] Processed 676600 reads in 209.025 CPU sec, 6.939 real sec
[M::mem_process_seqs] Processed 676800 reads in 219.712 CPU sec, 7.330 real sec
sorting block 0, #reads = 1082883 mem = 536878883 peak = 598675456
[M::process] read 676900 sequences (100000178 bp) in 2.521 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288412, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.20, 108.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676890 sequences (100000150 bp) in 1.590 CPU sec, 1.589 real sec...
[M::process] read 676934 sequences (100000214 bp) in 1.580 CPU sec, 1.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288506, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.79, 109.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 827, 827)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413)
[M::mem_pestat] mean and std.dev: (459.00, 380.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3206)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677112 reads in 206.324 CPU sec, 6.582 real sec
sorting block 1, #reads = 1082932 mem = 536880096 peak = 770117632
[M::mem_process_seqs] Processed 676900 reads in 120.891 CPU sec, 3.868 real sec
sorting block 2, #reads = 1082214 mem = 536880216 peak = 869408768
[M::process] read 676808 sequences (100000110 bp) in 2.083 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288254, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.18, 108.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676608 sequences (100000021 bp) in 1.340 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287973, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.36, 109.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287871, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.86, 110.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676890 reads in 301.220 CPU sec, 9.707 real sec
[M::mem_process_seqs] Processed 676934 reads in 212.739 CPU sec, 6.944 real sec
[M::mem_process_seqs] Processed 676808 reads in 224.609 CPU sec, 7.367 real sec
sorting block 3, #reads = 1082064 mem = 536879747 peak = 869408768
[M::process] read 676012 sequences (100000263 bp) in 2.584 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288416, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.58, 112.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1082613 mem = 536879513 peak = 869408768
[M::process] read 676200 sequences (100000115 bp) in 1.730 CPU sec, 1.801 real sec...
[M::process] read 676082 sequences (100000208 bp) in 1.354 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287285, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (243.00, 123.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676608 reads in 212.662 CPU sec, 6.804 real sec
[M::mem_process_seqs] Processed 676012 reads in 121.412 CPU sec, 3.891 real sec
sorting block 5, #reads = 1081936 mem = 536878014 peak = 869408768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287499, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.11, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676608 sequences (100000291 bp) in 2.758 CPU sec, 2.949 real sec...
[M::process] read 676838 sequences (100000125 bp) in 1.690 CPU sec, 1.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287897, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.62, 123.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 211, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (193.30, 222.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676200 reads in 205.284 CPU sec, 6.559 real sec
[M::mem_process_seqs] Processed 676082 reads in 117.510 CPU sec, 3.774 real sec
sorting block 6, #reads = 1081945 mem = 536878196 peak = 869408768
[M::process] read 676698 sequences (100000135 bp) in 1.865 CPU sec, 1.927 real sec...
[M::process] read 676830 sequences (100000262 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287822, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.56, 113.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 404, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288)
[M::mem_pestat] mean and std.dev: (294.20, 202.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1704)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676698 reads in 105.205 CPU sec, 3.394 real sec
sorting block 7, #reads = 1082502 mem = 536881855 peak = 869408768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287857, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.23, 114.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676940 sequences (100000204 bp) in 1.544 CPU sec, 1.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288255, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.58, 112.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676608 reads in 306.081 CPU sec, 9.824 real sec
[M::mem_process_seqs] Processed 676838 reads in 218.046 CPU sec, 7.035 real sec
sorting block 8, #reads = 1082087 mem = 536880542 peak = 869408768
[M::process] read 676906 sequences (100000240 bp) in 1.966 CPU sec, 2.097 real sec...
[M::process] read 677042 sequences (100000087 bp) in 1.590 CPU sec, 1.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288182, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.49, 109.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676940 reads in 102.734 CPU sec, 3.289 real sec
sorting block 9, #reads = 1082418 mem = 536879027 peak = 869408768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288392, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.42, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 13, 63)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163)
[M::mem_pestat] mean and std.dev: (30.73, 26.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 213)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676766 sequences (100000034 bp) in 1.691 CPU sec, 1.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288191, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.30, 109.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676830 reads in 293.703 CPU sec, 9.401 real sec
[M::mem_process_seqs] Processed 676906 reads in 214.163 CPU sec, 6.882 real sec
sorting block 10, #reads = 1082869 mem = 536879784 peak = 869408768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288147, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.95, 109.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676816 sequences (100000297 bp) in 2.416 CPU sec, 2.534 real sec...
[M::process] read 676332 sequences (100000108 bp) in 1.580 CPU sec, 1.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288126, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.11, 111.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677042 reads in 195.051 CPU sec, 6.227 real sec
[M::mem_process_seqs] Processed 676766 reads in 113.454 CPU sec, 3.651 real sec
sorting block 11, #reads = 1082406 mem = 536882352 peak = 869408768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288156, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.57, 112.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 71, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188)
[M::mem_pestat] mean and std.dev: (65.27, 26.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 237)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676490 sequences (100000185 bp) in 2.598 CPU sec, 2.705 real sec...
[M::process] read 676090 sequences (100000064 bp) in 1.720 CPU sec, 1.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287405, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.65, 121.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 11, 34)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 96)
[M::mem_pestat] mean and std.dev: (16.30, 15.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 127)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676816 reads in 199.554 CPU sec, 6.368 real sec
[M::mem_process_seqs] Processed 676332 reads in 116.069 CPU sec, 3.724 real sec
sorting block 12, #reads = 1082343 mem = 536879212 peak = 869408768
[M::process] read 676290 sequences (100000019 bp) in 2.120 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287468, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.02, 119.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676874 sequences (100000223 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288151, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (243.47, 124.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287719, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.41, 120.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 336, 1974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5498)
[M::mem_pestat] mean and std.dev: (1106.60, 1293.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7260)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676490 reads in 280.909 CPU sec, 9.038 real sec
sorting block 13, #reads = 1082271 mem = 536876019 peak = 869408768
[M::mem_process_seqs] Processed 676090 reads in 198.769 CPU sec, 6.450 real sec
[M::mem_process_seqs] Processed 676290 reads in 210.242 CPU sec, 6.861 real sec
sorting block 14, #reads = 1082051 mem = 536879702 peak = 889839616
[M::process] read 676660 sequences (100000012 bp) in 2.363 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288239, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.70, 110.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676682 sequences (100000046 bp) in 2.133 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287932, 17, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.95, 114.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 80, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1782)
[M::mem_pestat] mean and std.dev: (250.44, 273.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2359)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676890 sequences (100000226 bp) in 1.360 CPU sec, 1.358 real sec...
[M::mem_process_seqs] Processed 676874 reads in 192.822 CPU sec, 6.141 real sec
[M::mem_process_seqs] Processed 676660 reads in 112.143 CPU sec, 3.566 real sec
sorting block 15, #reads = 1081546 mem = 536876989 peak = 889839616
sorting block 16, #reads = 1082277 mem = 536879187 peak = 932966400
[M::process] read 677022 sequences (100000055 bp) in 1.839 CPU sec, 1.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287707, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.41, 114.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000045 bp) in 1.620 CPU sec, 1.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288075, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.59, 112.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288255, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.82, 109.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676682 reads in 269.726 CPU sec, 8.660 real sec
[M::mem_process_seqs] Processed 676890 reads in 191.656 CPU sec, 6.197 real sec
[M::mem_process_seqs] Processed 677022 reads in 202.342 CPU sec, 6.577 real sec
sorting block 17, #reads = 1081589 mem = 536877262 peak = 932966400
[M::process] read 676842 sequences (100000057 bp) in 2.194 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287937, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.58, 111.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676634 sequences (100000056 bp) in 1.997 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288242, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.03, 112.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676804 sequences (100000104 bp) in 1.720 CPU sec, 1.725 real sec...
[M::mem_process_seqs] Processed 676780 reads in 193.377 CPU sec, 6.140 real sec
sorting block 18, #reads = 1082610 mem = 536880522 peak = 932966400
[M::mem_process_seqs] Processed 676842 reads in 110.978 CPU sec, 3.528 real sec
sorting block 19, #reads = 1082429 mem = 536879975 peak = 941051904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287979, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.77, 113.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676456 sequences (100000117 bp) in 2.319 CPU sec, 2.553 real sec...
[M::process] read 676390 sequences (100000206 bp) in 1.560 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288047, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.84, 115.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676634 reads in 191.696 CPU sec, 6.130 real sec
[M::mem_process_seqs] Processed 676804 reads in 109.885 CPU sec, 3.537 real sec
sorting block 20, #reads = 1082785 mem = 536879651 peak = 941051904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288088, 14, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.62, 117.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 1766, 1766)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5256)
[M::mem_pestat] mean and std.dev: (933.64, 836.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7001)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676228 sequences (100000018 bp) in 1.993 CPU sec, 2.142 real sec...
[M::process] read 676534 sequences (100000270 bp) in 1.476 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288201, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.53, 118.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (57, 222, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (270.69, 253.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1421)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676456 reads in 176.930 CPU sec, 5.670 real sec
[M::mem_process_seqs] Processed 676390 reads in 95.825 CPU sec, 3.069 real sec
sorting block 21, #reads = 1082401 mem = 536879388 peak = 941051904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287730, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.83, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 12, 100)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290)
[M::mem_pestat] mean and std.dev: (36.75, 43.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 385)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676834 sequences (100000076 bp) in 2.047 CPU sec, 2.173 real sec...
[M::process] read 676656 sequences (100000235 bp) in 1.386 CPU sec, 1.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288319, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.89, 114.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676228 reads in 157.486 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 676534 reads in 89.466 CPU sec, 2.868 real sec
sorting block 22, #reads = 1082189 mem = 536879212 peak = 941051904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288664, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.66, 112.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676370 sequences (100000020 bp) in 2.161 CPU sec, 2.330 real sec...
[M::process] read 677004 sequences (100000120 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287891, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.68, 114.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676834 reads in 166.062 CPU sec, 5.301 real sec
sorting block 23, #reads = 1081639 mem = 536877398 peak = 941051904
[M::mem_process_seqs] Processed 676656 reads in 98.590 CPU sec, 3.154 real sec
sorting block 24, #reads = 1082351 mem = 536879165 peak = 972267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287912, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.61, 116.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676834 sequences (100000192 bp) in 2.679 CPU sec, 2.861 real sec...
[M::process] read 676738 sequences (100000015 bp) in 1.290 CPU sec, 1.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288212, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.98, 110.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676370 reads in 176.998 CPU sec, 5.648 real sec
[M::mem_process_seqs] Processed 677004 reads in 102.015 CPU sec, 3.266 real sec
sorting block 25, #reads = 1082139 mem = 536878183 peak = 972267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288421, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.74, 109.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676522 sequences (100000210 bp) in 2.015 CPU sec, 2.160 real sec...
[M::process] read 676600 sequences (100000046 bp) in 1.370 CPU sec, 1.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288239, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.10, 110.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 20, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300)
[M::mem_pestat] mean and std.dev: (47.64, 72.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 399)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676834 reads in 178.133 CPU sec, 5.713 real sec
[M::mem_process_seqs] Processed 676738 reads in 103.557 CPU sec, 3.339 real sec
sorting block 26, #reads = 1082848 mem = 536879445 peak = 972267520
[M::process] read 676478 sequences (100000214 bp) in 2.441 CPU sec, 2.626 real sec...
[M::process] read 676396 sequences (100000206 bp) in 1.770 CPU sec, 1.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288346, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.83, 113.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (392, 438, 1843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4745)
[M::mem_pestat] mean and std.dev: (663.23, 668.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6196)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676600 reads in 95.046 CPU sec, 3.012 real sec
sorting block 27, #reads = 1082638 mem = 536880028 peak = 972267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288331, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.24, 113.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676276 sequences (100000187 bp) in 1.456 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288087, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.45, 115.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 51, 1176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3504)
[M::mem_pestat] mean and std.dev: (651.10, 884.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4668)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676522 reads in 270.726 CPU sec, 8.648 real sec
[M::mem_process_seqs] Processed 676478 reads in 115.976 CPU sec, 3.767 real sec
sorting block 28, #reads = 1082328 mem = 536878189 peak = 972267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288176, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.73, 117.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676064 sequences (100000093 bp) in 2.094 CPU sec, 2.184 real sec...
[M::process] read 676918 sequences (100000145 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287938, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.50, 118.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676396 reads in 159.459 CPU sec, 5.087 real sec
[M::mem_process_seqs] Processed 676276 reads in 89.435 CPU sec, 2.860 real sec
sorting block 29, #reads = 1081788 mem = 536878358 peak = 972267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288236, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (239.26, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676920 sequences (100000276 bp) in 2.291 CPU sec, 2.517 real sec...
[M::process] read 677016 sequences (100000278 bp) in 1.340 CPU sec, 1.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287561, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.99, 111.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676064 reads in 160.910 CPU sec, 5.132 real sec
sorting block 30, #reads = 1081910 mem = 536878662 peak = 972267520
[M::mem_process_seqs] Processed 676918 reads in 94.605 CPU sec, 3.022 real sec
sorting block 31, #reads = 1081883 mem = 536878017 peak = 984797184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287232, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.45, 112.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677298 sequences (100000228 bp) in 2.008 CPU sec, 2.136 real sec...
[M::process] read 676958 sequences (100000121 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287299, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.81, 112.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676920 reads in 168.788 CPU sec, 5.392 real sec
[M::mem_process_seqs] Processed 677016 reads in 96.491 CPU sec, 3.086 real sec
sorting block 32, #reads = 1082030 mem = 536879524 peak = 984797184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288200, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.67, 107.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677140 sequences (100000200 bp) in 2.185 CPU sec, 2.274 real sec...
[M::process] read 677038 sequences (100000039 bp) in 2.040 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287407, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.37, 111.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 119)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351)
[M::mem_pestat] mean and std.dev: (25.25, 37.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677298 reads in 168.454 CPU sec, 5.343 real sec
[M::mem_process_seqs] Processed 676958 reads in 93.784 CPU sec, 2.972 real sec
sorting block 33, #reads = 1082364 mem = 536876203 peak = 984797184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287586, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.91, 106.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677072 sequences (100000290 bp) in 2.341 CPU sec, 2.384 real sec...
[M::process] read 676928 sequences (100000111 bp) in 1.507 CPU sec, 1.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287519, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.77, 106.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677140 reads in 167.301 CPU sec, 5.329 real sec
[M::mem_process_seqs] Processed 677038 reads in 94.944 CPU sec, 3.017 real sec
sorting block 34, #reads = 1082158 mem = 536879307 peak = 984797184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287753, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.05, 108.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676264 sequences (100000138 bp) in 2.035 CPU sec, 2.129 real sec...
[M::process] read 676504 sequences (100000001 bp) in 1.560 CPU sec, 1.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287539, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.62, 109.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677072 reads in 166.093 CPU sec, 5.292 real sec
sorting block 35, #reads = 1082510 mem = 536881546 peak = 984797184
[M::mem_process_seqs] Processed 676928 reads in 93.920 CPU sec, 3.000 real sec
sorting block 36, #reads = 1082501 mem = 536878480 peak = 1036894208
[M::process] read 676332 sequences (100000289 bp) in 1.917 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287461, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (238.71, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (96, 125, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426)
[M::mem_pestat] mean and std.dev: (106.20, 73.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677062 sequences (100000286 bp) in 1.600 CPU sec, 1.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287806, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.49, 115.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287590, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.75, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676264 reads in 244.256 CPU sec, 7.816 real sec
[M::mem_process_seqs] Processed 676504 reads in 170.884 CPU sec, 5.490 real sec
[M::mem_process_seqs] Processed 676332 reads in 178.740 CPU sec, 5.764 real sec
sorting block 37, #reads = 1082445 mem = 536878302 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288108, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.63, 110.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677062 reads in 86.502 CPU sec, 2.857 real sec
[M::process] read 677084 sequences (100000076 bp) in 2.712 CPU sec, 2.971 real sec...
sorting block 38, #reads = 1081651 mem = 536879172 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287942, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.99, 111.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676724 sequences (100000239 bp) in 2.454 CPU sec, 2.608 real sec...
[M::mem_process_seqs] Processed 677084 reads in 85.742 CPU sec, 2.781 real sec
sorting block 39, #reads = 1082289 mem = 536877943 peak = 1036894208
[M::process] read 677036 sequences (100000266 bp) in 2.194 CPU sec, 2.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287544, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.15, 110.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 89, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (164.29, 192.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677192 sequences (100000142 bp) in 1.530 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288049, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.68, 106.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 9, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (164.09, 254.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676724 reads in 159.594 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 677036 reads in 89.685 CPU sec, 2.822 real sec
[M::process] read 677050 sequences (100000045 bp) in 1.648 CPU sec, 1.658 real sec...
sorting block 40, #reads = 1082129 mem = 536880760 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288331, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.16, 106.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677132 sequences (100000025 bp) in 2.180 CPU sec, 2.287 real sec...
[M::process] read 676972 sequences (100000191 bp) in 1.600 CPU sec, 1.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287938, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.87, 107.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677192 reads in 159.908 CPU sec, 5.091 real sec
[M::mem_process_seqs] Processed 677050 reads in 90.888 CPU sec, 2.888 real sec
sorting block 41, #reads = 1082146 mem = 536879750 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288428, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.50, 107.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 26, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852)
[M::mem_pestat] mean and std.dev: (207.90, 287.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2468)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677038 sequences (100000077 bp) in 2.310 CPU sec, 2.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287985, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.11, 108.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676596 sequences (100000030 bp) in 1.970 CPU sec, 1.969 real sec...
[M::mem_process_seqs] Processed 677132 reads in 159.076 CPU sec, 5.040 real sec
sorting block 42, #reads = 1082807 mem = 536877869 peak = 1036894208
[M::mem_process_seqs] Processed 676972 reads in 91.888 CPU sec, 2.914 real sec
sorting block 43, #reads = 1082163 mem = 536879332 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287725, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.22, 109.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676856 sequences (100000195 bp) in 2.155 CPU sec, 2.210 real sec...
[M::process] read 676570 sequences (100000144 bp) in 1.620 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287352, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.78, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677038 reads in 166.080 CPU sec, 5.289 real sec
[M::mem_process_seqs] Processed 676596 reads in 95.617 CPU sec, 3.041 real sec
sorting block 44, #reads = 1082329 mem = 536880995 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287929, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.09, 113.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 89, 1484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4274)
[M::mem_pestat] mean and std.dev: (453.27, 631.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5669)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676960 sequences (100000131 bp) in 2.339 CPU sec, 2.420 real sec...
[M::process] read 676892 sequences (100000255 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287434, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.53, 117.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676856 reads in 163.411 CPU sec, 5.196 real sec
[M::mem_process_seqs] Processed 676570 reads in 92.986 CPU sec, 2.966 real sec
sorting block 45, #reads = 1081958 mem = 536879263 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288384, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.46, 110.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676730 sequences (100000216 bp) in 2.732 CPU sec, 2.801 real sec...
[M::process] read 676836 sequences (100000164 bp) in 1.580 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287783, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.07, 111.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676960 reads in 160.129 CPU sec, 5.084 real sec
[M::mem_process_seqs] Processed 676892 reads in 91.908 CPU sec, 2.924 real sec
sorting block 46, #reads = 1082337 mem = 536882330 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287932, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.54, 113.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 78, 9622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28822)
[M::mem_pestat] mean and std.dev: (2713.83, 4284.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38422)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677154 sequences (100000283 bp) in 2.193 CPU sec, 2.270 real sec...
[M::process] read 677012 sequences (100000151 bp) in 1.570 CPU sec, 1.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287978, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.73, 110.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 38, 632)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828)
[M::mem_pestat] mean and std.dev: (139.92, 233.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2426)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676730 reads in 168.466 CPU sec, 5.373 real sec
sorting block 47, #reads = 1081965 mem = 536877212 peak = 1036894208
[M::mem_process_seqs] Processed 676836 reads in 98.806 CPU sec, 3.161 real sec
sorting block 48, #reads = 1081790 mem = 536879847 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287838, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.38, 105.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677206 sequences (100000245 bp) in 2.495 CPU sec, 2.597 real sec...
[M::process] read 677158 sequences (100000073 bp) in 1.850 CPU sec, 1.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287999, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.02, 106.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677154 reads in 180.361 CPU sec, 5.745 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288317, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.60, 109.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 107, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (87.00, 98.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676818 sequences (100000110 bp) in 2.225 CPU sec, 2.226 real sec...
[M::mem_process_seqs] Processed 677206 reads in 95.252 CPU sec, 3.031 real sec
sorting block 49, #reads = 1082631 mem = 536881706 peak = 1036894208
[M::mem_process_seqs] Processed 677012 reads in 219.282 CPU sec, 6.989 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287967, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.89, 109.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1082741 mem = 536878162 peak = 1036894208
[M::process] read 676874 sequences (100000217 bp) in 2.980 CPU sec, 3.166 real sec...
[M::process] read 676900 sequences (100000091 bp) in 1.520 CPU sec, 1.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288103, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.97, 111.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 230, 8112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24316)
[M::mem_pestat] mean and std.dev: (2756.75, 3787.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32418)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677158 reads in 189.568 CPU sec, 6.039 real sec
[M::mem_process_seqs] Processed 676818 reads in 101.773 CPU sec, 3.235 real sec
sorting block 51, #reads = 1083027 mem = 536879869 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287949, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.43, 112.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676710 sequences (100000250 bp) in 2.269 CPU sec, 2.462 real sec...
[M::process] read 676830 sequences (100000058 bp) in 1.640 CPU sec, 1.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288048, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.31, 114.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676874 reads in 173.557 CPU sec, 5.536 real sec
[M::mem_process_seqs] Processed 676900 reads in 99.886 CPU sec, 3.188 real sec
sorting block 52, #reads = 1082490 mem = 536880115 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288168, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.91, 114.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677230 sequences (100000246 bp) in 2.206 CPU sec, 2.369 real sec...
[M::process] read 677002 sequences (100000165 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288671, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.14, 113.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676710 reads in 165.910 CPU sec, 5.294 real sec
[M::mem_process_seqs] Processed 676830 reads in 93.697 CPU sec, 2.996 real sec
sorting block 53, #reads = 1082667 mem = 536880196 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288360, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.51, 108.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676688 sequences (100000256 bp) in 2.164 CPU sec, 2.303 real sec...
[M::process] read 676984 sequences (100000151 bp) in 1.580 CPU sec, 1.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288364, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.26, 112.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677230 reads in 160.023 CPU sec, 5.098 real sec
[M::mem_process_seqs] Processed 677002 reads in 89.685 CPU sec, 2.858 real sec
sorting block 54, #reads = 1082320 mem = 536879115 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288261, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.66, 115.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1082667 mem = 536880150 peak = 1036894208
[M::process] read 677292 sequences (100000015 bp) in 2.798 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287913, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.73, 109.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 28, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (50.27, 58.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677076 sequences (100000235 bp) in 1.590 CPU sec, 1.595 real sec...
[M::mem_process_seqs] Processed 676688 reads in 163.737 CPU sec, 5.214 real sec
[M::mem_process_seqs] Processed 676984 reads in 95.128 CPU sec, 3.025 real sec
sorting block 56, #reads = 1082257 mem = 536878770 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288321, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.73, 103.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 8, 12)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20)
[M::mem_pestat] mean and std.dev: (8.50, 1.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 24)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677060 sequences (100000248 bp) in 2.612 CPU sec, 2.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288296, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.40, 104.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676916 sequences (100000283 bp) in 1.570 CPU sec, 1.567 real sec...
[M::mem_process_seqs] Processed 677292 reads in 160.495 CPU sec, 5.113 real sec
[M::mem_process_seqs] Processed 677076 reads in 92.620 CPU sec, 2.944 real sec
sorting block 57, #reads = 1082515 mem = 536878477 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287945, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.40, 110.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676776 sequences (100000208 bp) in 2.436 CPU sec, 2.629 real sec...
[M::process] read 676750 sequences (100000036 bp) in 1.560 CPU sec, 1.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288067, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.30, 111.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677060 reads in 162.018 CPU sec, 5.169 real sec
[M::mem_process_seqs] Processed 676916 reads in 94.187 CPU sec, 3.016 real sec
sorting block 58, #reads = 1082310 mem = 536878674 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287923, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.83, 112.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676546 sequences (100000137 bp) in 1.979 CPU sec, 2.081 real sec...
[M::process] read 676634 sequences (100000001 bp) in 1.700 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287968, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.92, 114.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676776 reads in 157.460 CPU sec, 5.018 real sec
sorting block 59, #reads = 1082529 mem = 536878170 peak = 1036894208
[M::mem_process_seqs] Processed 676750 reads in 90.147 CPU sec, 2.886 real sec
[M::process] read 182714 sequences (27004017 bp) in 1.176 CPU sec, 1.244 real sec...
sorting block 60, #reads = 1082749 mem = 536877569 peak = 1036894208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287652, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.53, 115.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288163, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.57, 117.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 77726, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.08, 116.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676546 reads in 175.146 CPU sec, 5.675 real sec
[M::mem_process_seqs] Processed 676634 reads in 109.097 CPU sec, 3.560 real sec
[M::mem_process_seqs] Processed 182714 reads in 111.461 CPU sec, 3.645 real sec
sorting block 61, #reads = 1082103 mem = 536878291 peak = 1036894208
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67183570
overall: 69686280192 mem 10556.132 user 624.179 sys 380.144 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L005\tPU:L005\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T21-D0003376_R1.fastq.gz 0004.T21-D0003376_R2.fastq.gz
[main] Real time: 380.182 sec; CPU: 11180.313 sec
sorting block 62, #reads = 599294 mem = 297291710 peak = 1036894208
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2620789125
output reads: 67702756
bam_mem_sort: 63 calls 30.494 user 0.714 sys 31.287 real
bam_write: 63 calls 19.732 user 1.171 sys 21.015 real
execute: 1 calls 15.386 user 0.549 sys 397.560 real
merge_files: 1 calls 15.368 user 0.511 sys 16.886 real
parse_chunk: 1001 calls 51.445 user 12.376 sys 64.508 real
read_chunk: 2696 calls 27.974 user 18.839 sys 310.785 real
sort_block: 1 calls 19.272 user 18.544 sys 297.868 real
write_chunk: 1693 calls 15.047 user 0.313 sys 16.158 real
overall: 2449743872 mem 705.828 user 42.618 sys 397.583 real
ls: cannot access '*.cram': No such file or directory