.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003322;L006;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/77/21766b343530152ee01bac9e367475/.command.log
Size: 344.9 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003322;L006;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b7/faeb854553d10446f2724293243191/T21-D0003322/T21-D0003322_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/77/21766b343530152ee01bac9e367475/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b7/faeb854553d10446f2724293243191/T21-D0003322/T21-D0003322_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/77/21766b343530152ee01bac9e367475/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003322.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2237.232 CPU sec, 84.346 real sec
[M::process] read 675902 sequences (100000209 bp) in 0.908 CPU sec, 0.909 real sec...
[M::process] read 675864 sequences (100000209 bp) in 1.107 CPU sec, 1.142 real sec...
[M::process] read 675964 sequences (100000291 bp) in 1.175 CPU sec, 1.198 real sec...
[M::process] read 675870 sequences (100000263 bp) in 1.117 CPU sec, 1.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287102, 28, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (237.32, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 145, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (174.64, 185.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287089, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.50, 115.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286748, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (239.07, 114.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675902 reads in 316.776 CPU sec, 10.246 real sec
[M::mem_process_seqs] Processed 675964 reads in 191.260 CPU sec, 6.242 real sec
[M::mem_process_seqs] Processed 675864 reads in 205.532 CPU sec, 6.746 real sec
sorting block 0, #reads = 1081296 mem = 536875277 peak = 584351744
[M::process] read 675856 sequences (100000248 bp) in 1.631 CPU sec, 1.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286624, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.81, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 25, 72)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184)
[M::mem_pestat] mean and std.dev: (44.11, 52.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 253)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675618 sequences (100000016 bp) in 1.230 CPU sec, 1.317 real sec...
[M::process] read 675756 sequences (100000141 bp) in 0.959 CPU sec, 0.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286758, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.50, 116.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675870 reads in 180.454 CPU sec, 5.810 real sec
sorting block 1, #reads = 1080433 mem = 536877629 peak = 763658240
[M::mem_process_seqs] Processed 675856 reads in 110.204 CPU sec, 3.581 real sec
sorting block 2, #reads = 1081031 mem = 536877912 peak = 888270848
[M::process] read 675522 sequences (100000271 bp) in 1.965 CPU sec, 2.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286656, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (242.17, 117.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 165, 2339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6989)
[M::mem_pestat] mean and std.dev: (931.31, 1114.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9314)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675616 sequences (100000014 bp) in 0.990 CPU sec, 0.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286611, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.43, 117.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 32, 926)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2734)
[M::mem_pestat] mean and std.dev: (437.64, 453.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3638)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286697, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.45, 117.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675618 reads in 259.845 CPU sec, 8.387 real sec
[M::mem_process_seqs] Processed 675756 reads in 189.931 CPU sec, 6.189 real sec
[M::mem_process_seqs] Processed 675522 reads in 204.100 CPU sec, 6.695 real sec
sorting block 3, #reads = 1080812 mem = 536879298 peak = 888270848
[M::process] read 675498 sequences (100000166 bp) in 1.740 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286143, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.93, 118.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 61, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (123.86, 177.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 4, #reads = 1080723 mem = 536880101 peak = 888270848
[M::process] read 675526 sequences (100000060 bp) in 1.613 CPU sec, 1.769 real sec...
[M::process] read 675418 sequences (100000067 bp) in 0.955 CPU sec, 0.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286404, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.98, 118.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 58, 634)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802)
[M::mem_pestat] mean and std.dev: (218.55, 270.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2386)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675616 reads in 189.418 CPU sec, 6.112 real sec
[M::mem_process_seqs] Processed 675498 reads in 114.221 CPU sec, 3.707 real sec
sorting block 5, #reads = 1081011 mem = 536879451 peak = 888270848
[M::process] read 675316 sequences (100000111 bp) in 1.544 CPU sec, 1.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286690, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.04, 118.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675438 sequences (100000293 bp) in 0.990 CPU sec, 0.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286153, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.40, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286737, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.29, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 15, 674)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2000)
[M::mem_pestat] mean and std.dev: (277.54, 334.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2663)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675526 reads in 264.977 CPU sec, 8.564 real sec
[M::mem_process_seqs] Processed 675418 reads in 193.258 CPU sec, 6.311 real sec
[M::mem_process_seqs] Processed 675316 reads in 207.927 CPU sec, 6.836 real sec
sorting block 6, #reads = 1080599 mem = 536877648 peak = 888270848
[M::process] read 675410 sequences (100000224 bp) in 1.774 CPU sec, 1.919 real sec...
sorting block 7, #reads = 1080821 mem = 536880122 peak = 888270848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286324, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (246.85, 122.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675238 sequences (100000162 bp) in 1.596 CPU sec, 1.708 real sec...
[M::process] read 675212 sequences (100000035 bp) in 0.990 CPU sec, 0.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286550, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.14, 122.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675438 reads in 190.774 CPU sec, 6.153 real sec
[M::mem_process_seqs] Processed 675410 reads in 116.273 CPU sec, 3.786 real sec
sorting block 8, #reads = 1080721 mem = 536880683 peak = 888270848
[M::process] read 675272 sequences (100000101 bp) in 1.721 CPU sec, 1.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286296, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.25, 123.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675208 sequences (100000295 bp) in 0.998 CPU sec, 1.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286128, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.35, 124.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 61, 2986)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8886)
[M::mem_pestat] mean and std.dev: (1195.85, 1520.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11836)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285709, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.62, 123.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675238 reads in 273.826 CPU sec, 8.851 real sec
[M::mem_process_seqs] Processed 675272 reads in 198.161 CPU sec, 6.468 real sec
sorting block 9, #reads = 1080553 mem = 536879367 peak = 888270848
[M::mem_process_seqs] Processed 675212 reads in 213.026 CPU sec, 7.000 real sec
[M::process] read 675094 sequences (100000208 bp) in 2.231 CPU sec, 2.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285841, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.72, 124.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 39, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696)
[M::mem_pestat] mean and std.dev: (205.80, 254.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2257)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 10, #reads = 1080712 mem = 536879682 peak = 888270848
[M::process] read 675156 sequences (100000202 bp) in 1.434 CPU sec, 1.562 real sec...
[M::process] read 675156 sequences (100000089 bp) in 0.970 CPU sec, 0.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286319, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.70, 125.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 229, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (123.11, 125.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675208 reads in 198.200 CPU sec, 6.421 real sec
[M::mem_process_seqs] Processed 675094 reads in 118.968 CPU sec, 3.859 real sec
sorting block 11, #reads = 1080586 mem = 536881822 peak = 888270848
[M::process] read 675224 sequences (100000151 bp) in 1.714 CPU sec, 1.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286337, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.41, 126.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675076 sequences (100000165 bp) in 0.980 CPU sec, 0.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286029, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.19, 126.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285781, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.53, 125.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 645, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1851)
[M::mem_pestat] mean and std.dev: (348.56, 287.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2454)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675156 reads in 278.878 CPU sec, 9.011 real sec
[M::mem_process_seqs] Processed 675156 reads in 202.375 CPU sec, 6.600 real sec
sorting block 12, #reads = 1080646 mem = 536880115 peak = 888270848
[M::mem_process_seqs] Processed 675224 reads in 216.174 CPU sec, 7.096 real sec
[M::process] read 675050 sequences (100000082 bp) in 2.038 CPU sec, 2.379 real sec...
sorting block 13, #reads = 1080819 mem = 536879627 peak = 888270848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286168, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.35, 126.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675080 sequences (100000178 bp) in 2.385 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286360, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.28, 126.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 15, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (39.09, 80.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675022 sequences (100000061 bp) in 1.130 CPU sec, 1.130 real sec...
[M::mem_process_seqs] Processed 675076 reads in 194.400 CPU sec, 6.265 real sec
[M::mem_process_seqs] Processed 675050 reads in 119.525 CPU sec, 3.857 real sec
sorting block 14, #reads = 1080393 mem = 536878840 peak = 888270848
[M::process] read 675016 sequences (100000114 bp) in 2.227 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286130, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.55, 128.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 390, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1536)
[M::mem_pestat] mean and std.dev: (363.73, 322.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2034)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674990 sequences (100000183 bp) in 1.169 CPU sec, 1.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286150, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.11, 129.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286290, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.93, 128.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675080 reads in 278.479 CPU sec, 8.986 real sec
sorting block 15, #reads = 1080775 mem = 536878126 peak = 888270848
[M::mem_process_seqs] Processed 675016 reads in 200.951 CPU sec, 6.534 real sec
[M::mem_process_seqs] Processed 675022 reads in 215.744 CPU sec, 7.064 real sec
sorting block 16, #reads = 1080819 mem = 536879049 peak = 888270848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286205, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.91, 129.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 954, 2465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7369)
[M::mem_pestat] mean and std.dev: (1197.50, 1071.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9821)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674966 sequences (100000013 bp) in 2.928 CPU sec, 3.341 real sec...
[M::mem_process_seqs] Processed 674990 reads in 106.117 CPU sec, 3.538 real sec
sorting block 17, #reads = 1080770 mem = 536879373 peak = 888270848
[M::process] read 674986 sequences (100000026 bp) in 1.801 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286222, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.86, 129.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675036 sequences (100000191 bp) in 1.267 CPU sec, 1.305 real sec...
[M::process] read 674790 sequences (100000224 bp) in 1.030 CPU sec, 1.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286073, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.21, 129.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674966 reads in 187.392 CPU sec, 6.020 real sec
[M::mem_process_seqs] Processed 674986 reads in 113.593 CPU sec, 3.694 real sec
sorting block 18, #reads = 1080659 mem = 536878131 peak = 888270848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286052, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.35, 130.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674914 sequences (100000002 bp) in 2.257 CPU sec, 2.570 real sec...
[M::process] read 674776 sequences (100000189 bp) in 1.047 CPU sec, 1.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285601, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.12, 130.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 55, 833)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2477)
[M::mem_pestat] mean and std.dev: (252.91, 356.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3299)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675036 reads in 183.539 CPU sec, 5.898 real sec
[M::mem_process_seqs] Processed 674790 reads in 112.678 CPU sec, 3.657 real sec
sorting block 19, #reads = 1080240 mem = 536880411 peak = 888270848
[M::process] read 674742 sequences (100000138 bp) in 1.839 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285859, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.41, 129.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 3, 933)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2797)
[M::mem_pestat] mean and std.dev: (430.35, 703.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3729)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674724 sequences (100000245 bp) in 1.110 CPU sec, 1.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286347, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.61, 131.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285814, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.21, 130.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 242, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1489)
[M::mem_pestat] mean and std.dev: (260.60, 207.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1956)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674914 reads in 264.739 CPU sec, 8.524 real sec
sorting block 20, #reads = 1080432 mem = 536880056 peak = 888270848
[M::mem_process_seqs] Processed 674776 reads in 193.558 CPU sec, 6.289 real sec
[M::mem_process_seqs] Processed 674742 reads in 208.708 CPU sec, 6.832 real sec
sorting block 21, #reads = 1080584 mem = 536877155 peak = 900665344
[M::process] read 674742 sequences (100000248 bp) in 2.058 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286105, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.16, 132.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674704 sequences (100000261 bp) in 1.613 CPU sec, 1.732 real sec...
[M::process] read 674732 sequences (100000237 bp) in 0.990 CPU sec, 0.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286308, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.58, 132.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674724 reads in 186.350 CPU sec, 5.998 real sec
[M::mem_process_seqs] Processed 674742 reads in 115.851 CPU sec, 3.744 real sec
sorting block 22, #reads = 1080693 mem = 536881091 peak = 900665344
[M::process] read 674638 sequences (100000179 bp) in 1.844 CPU sec, 2.046 real sec...
[M::process] read 674726 sequences (100000252 bp) in 1.080 CPU sec, 1.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285744, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.28, 133.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674732 reads in 98.663 CPU sec, 3.179 real sec
sorting block 23, #reads = 1080307 mem = 536879485 peak = 900665344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286053, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.30, 132.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 18, 128)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350)
[M::mem_pestat] mean and std.dev: (69.07, 90.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 461)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674620 sequences (100000222 bp) in 1.014 CPU sec, 1.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285759, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.49, 134.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674704 reads in 282.534 CPU sec, 9.097 real sec
[M::mem_process_seqs] Processed 674638 reads in 210.667 CPU sec, 6.832 real sec
sorting block 24, #reads = 1080047 mem = 536879067 peak = 969789440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285577, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.28, 135.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674612 sequences (100000264 bp) in 2.019 CPU sec, 2.230 real sec...
[M::process] read 674566 sequences (100000135 bp) in 1.140 CPU sec, 1.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286046, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.29, 134.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674726 reads in 182.374 CPU sec, 5.856 real sec
sorting block 25, #reads = 1080279 mem = 536878845 peak = 969789440
[M::mem_process_seqs] Processed 674620 reads in 112.441 CPU sec, 3.652 real sec
sorting block 26, #reads = 1080055 mem = 536877803 peak = 969789440
[M::process] read 674506 sequences (100000015 bp) in 1.678 CPU sec, 1.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286013, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.43, 136.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674496 sequences (100000080 bp) in 1.100 CPU sec, 1.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286270, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.67, 136.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286239, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.74, 135.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 66, 4150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12318)
[M::mem_pestat] mean and std.dev: (1582.18, 1945.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16402)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674612 reads in 260.857 CPU sec, 8.404 real sec
[M::mem_process_seqs] Processed 674506 reads in 190.133 CPU sec, 6.167 real sec
[M::mem_process_seqs] Processed 674566 reads in 204.526 CPU sec, 6.682 real sec
sorting block 27, #reads = 1080516 mem = 536879813 peak = 969789440
[M::process] read 675214 sequences (100000175 bp) in 2.449 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286038, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.01, 136.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 28, #reads = 1080440 mem = 536880363 peak = 969789440
[M::process] read 675918 sequences (100000086 bp) in 1.563 CPU sec, 1.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286582, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.87, 128.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675770 sequences (100000275 bp) in 1.120 CPU sec, 1.120 real sec...
[M::mem_process_seqs] Processed 674496 reads in 184.540 CPU sec, 5.948 real sec
[M::mem_process_seqs] Processed 675214 reads in 113.070 CPU sec, 3.645 real sec
sorting block 29, #reads = 1080176 mem = 536879002 peak = 969789440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287525, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.42, 115.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 63, 1542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4592)
[M::mem_pestat] mean and std.dev: (705.75, 1183.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6117)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675738 sequences (100000119 bp) in 2.350 CPU sec, 2.600 real sec...
[M::process] read 675696 sequences (100000134 bp) in 1.250 CPU sec, 1.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287325, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.93, 116.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675918 reads in 181.417 CPU sec, 5.819 real sec
[M::mem_process_seqs] Processed 675770 reads in 113.044 CPU sec, 3.659 real sec
sorting block 30, #reads = 1081437 mem = 536878509 peak = 969789440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286660, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.96, 116.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675728 sequences (100000223 bp) in 2.012 CPU sec, 2.263 real sec...
[M::process] read 675564 sequences (100000059 bp) in 1.240 CPU sec, 1.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286837, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (242.06, 117.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 61, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455)
[M::mem_pestat] mean and std.dev: (220.27, 241.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1929)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675738 reads in 182.771 CPU sec, 5.872 real sec
[M::mem_process_seqs] Processed 675696 reads in 112.876 CPU sec, 3.660 real sec
sorting block 31, #reads = 1081088 mem = 536876990 peak = 969789440
[M::process] read 675626 sequences (100000142 bp) in 1.860 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286778, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.95, 118.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 26, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (122.00, 132.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675704 sequences (100000282 bp) in 1.330 CPU sec, 1.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286814, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.84, 118.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286646, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.14, 119.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 87, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (137.60, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675728 reads in 262.809 CPU sec, 8.451 real sec
sorting block 32, #reads = 1081049 mem = 536879187 peak = 969789440
[M::mem_process_seqs] Processed 675564 reads in 192.437 CPU sec, 6.228 real sec
[M::mem_process_seqs] Processed 675626 reads in 207.237 CPU sec, 6.759 real sec
sorting block 33, #reads = 1081282 mem = 536878242 peak = 969789440
[M::process] read 675558 sequences (100000008 bp) in 2.314 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286490, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.28, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675394 sequences (100000290 bp) in 2.154 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286125, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.79, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675704 reads in 190.802 CPU sec, 6.139 real sec
[M::process] read 675584 sequences (100000204 bp) in 1.030 CPU sec, 1.026 real sec...
[M::mem_process_seqs] Processed 675558 reads in 117.830 CPU sec, 3.792 real sec
sorting block 34, #reads = 1081063 mem = 536878348 peak = 969789440
[M::process] read 675432 sequences (100000173 bp) in 1.846 CPU sec, 2.033 real sec...
[M::process] read 675312 sequences (100000283 bp) in 1.000 CPU sec, 1.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286465, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (246.23, 120.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675584 reads in 101.282 CPU sec, 3.269 real sec
sorting block 35, #reads = 1080872 mem = 536876833 peak = 969789440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286453, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.87, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675324 sequences (100000136 bp) in 1.002 CPU sec, 1.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286163, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.21, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675394 reads in 284.519 CPU sec, 9.165 real sec
[M::mem_process_seqs] Processed 675432 reads in 213.392 CPU sec, 6.922 real sec
sorting block 36, #reads = 1080741 mem = 536879943 peak = 1005346816
[M::process] read 675438 sequences (100000014 bp) in 1.904 CPU sec, 2.077 real sec...
[M::process] read 675294 sequences (100000120 bp) in 0.980 CPU sec, 0.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286063, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.31, 122.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675438 reads in 98.657 CPU sec, 3.187 real sec
sorting block 37, #reads = 1080908 mem = 536878882 peak = 1005346816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286185, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.09, 121.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 85, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475)
[M::mem_pestat] mean and std.dev: (276.70, 312.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1959)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675328 sequences (100000034 bp) in 1.393 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286128, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (248.12, 122.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 37, 1385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4081)
[M::mem_pestat] mean and std.dev: (518.55, 819.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5429)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675312 reads in 282.554 CPU sec, 9.083 real sec
[M::mem_process_seqs] Processed 675324 reads in 212.039 CPU sec, 6.869 real sec
sorting block 38, #reads = 1080322 mem = 536879248 peak = 1005346816
[M::process] read 675290 sequences (100000021 bp) in 1.911 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286182, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.24, 124.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675202 sequences (100000083 bp) in 1.180 CPU sec, 1.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286240, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.97, 123.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285827, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.96, 125.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675294 reads in 266.957 CPU sec, 8.587 real sec
[M::mem_process_seqs] Processed 675328 reads in 196.998 CPU sec, 6.390 real sec
sorting block 39, #reads = 1080586 mem = 536880623 peak = 1005346816
[M::mem_process_seqs] Processed 675290 reads in 210.667 CPU sec, 6.880 real sec
sorting block 40, #reads = 1080774 mem = 536878984 peak = 1005346816
[M::process] read 675142 sequences (100000233 bp) in 1.811 CPU sec, 2.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286052, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.19, 125.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675112 sequences (100000163 bp) in 1.601 CPU sec, 1.720 real sec...
[M::process] read 675236 sequences (100000070 bp) in 1.033 CPU sec, 1.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285888, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.67, 126.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675202 reads in 187.514 CPU sec, 6.027 real sec
[M::mem_process_seqs] Processed 675142 reads in 114.804 CPU sec, 3.713 real sec
sorting block 41, #reads = 1080829 mem = 536879087 peak = 1005346816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285908, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.15, 126.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675380 sequences (100000037 bp) in 2.669 CPU sec, 3.135 real sec...
[M::process] read 675220 sequences (100000224 bp) in 1.000 CPU sec, 1.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286290, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.28, 126.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675112 reads in 187.941 CPU sec, 6.028 real sec
sorting block 42, #reads = 1080315 mem = 536880557 peak = 1005346816
[M::mem_process_seqs] Processed 675236 reads in 114.586 CPU sec, 3.718 real sec
sorting block 43, #reads = 1080932 mem = 536879848 peak = 1005346816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286127, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.18, 125.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 31, 121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337)
[M::mem_pestat] mean and std.dev: (53.56, 60.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 445)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675108 sequences (100000177 bp) in 2.018 CPU sec, 2.326 real sec...
[M::process] read 675028 sequences (100000274 bp) in 1.040 CPU sec, 1.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286303, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.75, 126.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 221, 658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1766)
[M::mem_pestat] mean and std.dev: (238.82, 232.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2320)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675380 reads in 185.179 CPU sec, 5.955 real sec
[M::mem_process_seqs] Processed 675220 reads in 113.826 CPU sec, 3.692 real sec
sorting block 44, #reads = 1080934 mem = 536880234 peak = 1005346816
[M::process] read 675056 sequences (100000122 bp) in 1.597 CPU sec, 1.747 real sec...
[M::process] read 675080 sequences (100000235 bp) in 1.040 CPU sec, 1.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286093, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.85, 129.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675056 reads in 100.644 CPU sec, 3.248 real sec
sorting block 45, #reads = 1080886 mem = 536878276 peak = 1005346816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286428, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.42, 127.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (833, 1989, 1989)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301)
[M::mem_pestat] mean and std.dev: (1360.60, 813.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5457)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674986 sequences (100000103 bp) in 1.064 CPU sec, 1.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285719, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.53, 128.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675108 reads in 287.073 CPU sec, 9.256 real sec
[M::mem_process_seqs] Processed 675028 reads in 214.244 CPU sec, 6.950 real sec
sorting block 46, #reads = 1080632 mem = 536879710 peak = 1005346816
[M::process] read 674908 sequences (100000123 bp) in 1.717 CPU sec, 1.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286118, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.58, 128.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 674, 888)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654)
[M::mem_pestat] mean and std.dev: (558.29, 614.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3537)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674812 sequences (100000210 bp) in 1.020 CPU sec, 1.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286043, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.70, 130.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286512, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.29, 129.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675080 reads in 265.402 CPU sec, 8.551 real sec
[M::mem_process_seqs] Processed 674986 reads in 194.410 CPU sec, 6.308 real sec
[M::mem_process_seqs] Processed 674908 reads in 209.198 CPU sec, 6.838 real sec
sorting block 47, #reads = 1081348 mem = 536880026 peak = 1005346816
[M::process] read 674822 sequences (100000101 bp) in 2.366 CPU sec, 2.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285970, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.21, 130.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1081149 mem = 536878527 peak = 1005346816
[M::process] read 674934 sequences (100000260 bp) in 2.149 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286093, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.11, 130.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 97, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686)
[M::mem_pestat] mean and std.dev: (249.90, 265.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2242)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674812 reads in 187.153 CPU sec, 6.026 real sec
[M::mem_process_seqs] Processed 674822 reads in 115.994 CPU sec, 3.718 real sec
[M::process] read 674890 sequences (100000128 bp) in 1.598 CPU sec, 1.741 real sec...
sorting block 49, #reads = 1080808 mem = 536881367 peak = 1005346816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286105, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.37, 132.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674738 sequences (100000064 bp) in 1.733 CPU sec, 1.922 real sec...
[M::process] read 674836 sequences (100000188 bp) in 1.000 CPU sec, 0.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285858, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.51, 132.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674934 reads in 184.263 CPU sec, 5.938 real sec
[M::mem_process_seqs] Processed 674890 reads in 113.230 CPU sec, 3.679 real sec
sorting block 50, #reads = 1080397 mem = 536878769 peak = 1005346816
[M::process] read 674634 sequences (100000241 bp) in 1.669 CPU sec, 1.862 real sec...
[M::process] read 674752 sequences (100000015 bp) in 1.050 CPU sec, 1.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286163, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.36, 131.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 32, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (167.23, 243.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1558)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674836 reads in 100.632 CPU sec, 3.253 real sec
sorting block 51, #reads = 1080435 mem = 536877478 peak = 1005346816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285379, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.68, 131.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 27, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (169.21, 236.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1423)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674588 sequences (100000116 bp) in 0.999 CPU sec, 1.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285888, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.35, 132.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674738 reads in 283.898 CPU sec, 9.148 real sec
[M::mem_process_seqs] Processed 674634 reads in 211.849 CPU sec, 6.874 real sec
sorting block 52, #reads = 1080681 mem = 536880564 peak = 1005346816
[M::process] read 674624 sequences (100000187 bp) in 1.865 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285646, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.92, 132.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 343, 920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2584)
[M::mem_pestat] mean and std.dev: (339.77, 343.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3416)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674588 sequences (100000042 bp) in 1.030 CPU sec, 1.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285757, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.16, 133.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 980, 4963)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14751)
[M::mem_pestat] mean and std.dev: (2140.80, 2319.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19645)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674752 reads in 184.540 CPU sec, 5.949 real sec
[M::mem_process_seqs] Processed 674588 reads in 113.206 CPU sec, 3.686 real sec
sorting block 53, #reads = 1080428 mem = 536881165 peak = 1005346816
[M::process] read 674694 sequences (100000194 bp) in 1.941 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285388, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.98, 133.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674536 sequences (100000210 bp) in 1.020 CPU sec, 1.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285937, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.05, 136.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286227, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.55, 134.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674624 reads in 263.978 CPU sec, 8.495 real sec
sorting block 54, #reads = 1080074 mem = 536879925 peak = 1005346816
[M::mem_process_seqs] Processed 674694 reads in 193.433 CPU sec, 6.270 real sec
[M::mem_process_seqs] Processed 674588 reads in 208.247 CPU sec, 6.799 real sec
sorting block 55, #reads = 1080192 mem = 536879317 peak = 1019297792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285593, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.56, 137.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674382 sequences (100000237 bp) in 2.479 CPU sec, 2.909 real sec...
[M::mem_process_seqs] Processed 674536 reads in 102.399 CPU sec, 3.411 real sec
[M::process] read 674598 sequences (100000218 bp) in 1.883 CPU sec, 2.115 real sec...
sorting block 56, #reads = 1080370 mem = 536877751 peak = 1019297792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285738, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.42, 137.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 23, 1133)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3363)
[M::mem_pestat] mean and std.dev: (530.77, 557.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4478)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674622 sequences (100000240 bp) in 1.571 CPU sec, 1.657 real sec...
[M::process] read 674546 sequences (100000131 bp) in 1.070 CPU sec, 1.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285565, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.91, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674382 reads in 184.472 CPU sec, 5.910 real sec
[M::mem_process_seqs] Processed 674598 reads in 113.103 CPU sec, 3.665 real sec
sorting block 57, #reads = 1080133 mem = 536881208 peak = 1019297792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285684, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.80, 136.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675586 sequences (100000270 bp) in 2.517 CPU sec, 2.874 real sec...
[M::process] read 675840 sequences (100000179 bp) in 1.050 CPU sec, 1.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285908, 13, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (266.89, 138.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 113, 2702)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8072)
[M::mem_pestat] mean and std.dev: (930.85, 1157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10757)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674622 reads in 182.179 CPU sec, 5.849 real sec
[M::mem_process_seqs] Processed 674546 reads in 112.432 CPU sec, 3.638 real sec
sorting block 58, #reads = 1079689 mem = 536879529 peak = 1019297792
[M::process] read 675796 sequences (100000286 bp) in 1.538 CPU sec, 1.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286814, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.05, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675746 sequences (100000279 bp) in 1.040 CPU sec, 1.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286400, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (240.62, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 121, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (76.18, 69.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286494, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (240.14, 115.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675586 reads in 259.284 CPU sec, 8.354 real sec
sorting block 59, #reads = 1079319 mem = 536877773 peak = 1023766528
[M::mem_process_seqs] Processed 675796 reads in 190.330 CPU sec, 6.177 real sec
[M::mem_process_seqs] Processed 675840 reads in 204.934 CPU sec, 6.700 real sec
sorting block 60, #reads = 1081144 mem = 536880660 peak = 1054867456
[M::process] read 675776 sequences (100000248 bp) in 2.035 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286133, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.17, 117.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 5, 90)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262)
[M::mem_pestat] mean and std.dev: (17.09, 25.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 348)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675698 sequences (100000218 bp) in 1.483 CPU sec, 1.591 real sec...
[M::process] read 675718 sequences (100000174 bp) in 1.040 CPU sec, 1.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286768, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.86, 118.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (105, 240, 1414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4032)
[M::mem_pestat] mean and std.dev: (506.55, 565.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5341)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675746 reads in 186.576 CPU sec, 6.001 real sec
[M::mem_process_seqs] Processed 675776 reads in 114.184 CPU sec, 3.687 real sec
sorting block 61, #reads = 1080830 mem = 536877039 peak = 1054867456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286979, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.47, 117.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 62, #reads = 1080842 mem = 536877856 peak = 1054867456
[M::process] read 675570 sequences (100000297 bp) in 2.575 CPU sec, 3.067 real sec...
[M::process] read 675542 sequences (100000126 bp) in 1.092 CPU sec, 1.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286725, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.32, 119.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675698 reads in 183.654 CPU sec, 5.897 real sec
[M::mem_process_seqs] Processed 675718 reads in 115.022 CPU sec, 3.721 real sec
sorting block 63, #reads = 1080782 mem = 536880891 peak = 1054867456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286374, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.69, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675538 sequences (100000014 bp) in 2.128 CPU sec, 2.461 real sec...
[M::process] read 675484 sequences (100000221 bp) in 1.040 CPU sec, 1.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286376, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.59, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675570 reads in 184.424 CPU sec, 5.925 real sec
[M::mem_process_seqs] Processed 675542 reads in 114.046 CPU sec, 3.702 real sec
sorting block 64, #reads = 1081181 mem = 536878670 peak = 1054867456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286315, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.80, 120.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 215, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (176.92, 127.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675380 sequences (100000237 bp) in 2.180 CPU sec, 2.528 real sec...
[M::process] read 675428 sequences (100000244 bp) in 1.070 CPU sec, 1.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286432, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.55, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 25, 600)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1780)
[M::mem_pestat] mean and std.dev: (149.00, 237.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2370)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675538 reads in 184.816 CPU sec, 5.942 real sec
[M::mem_process_seqs] Processed 675484 reads in 114.669 CPU sec, 3.718 real sec
sorting block 65, #reads = 1080762 mem = 536880179 peak = 1054867456
[M::process] read 675342 sequences (100000031 bp) in 1.654 CPU sec, 1.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286106, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (246.86, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 26, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140)
[M::mem_pestat] mean and std.dev: (163.50, 237.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675396 sequences (100000059 bp) in 1.070 CPU sec, 1.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286575, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.79, 120.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 1494, 2327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6945)
[M::mem_pestat] mean and std.dev: (1308.11, 1035.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9254)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286276, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.58, 121.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 39, 1135)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3371)
[M::mem_pestat] mean and std.dev: (405.00, 478.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4489)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675380 reads in 263.928 CPU sec, 8.511 real sec
sorting block 66, #reads = 1080723 mem = 536881357 peak = 1054867456
[M::mem_process_seqs] Processed 675428 reads in 192.343 CPU sec, 6.248 real sec
[M::mem_process_seqs] Processed 675342 reads in 206.958 CPU sec, 6.774 real sec
sorting block 67, #reads = 1080806 mem = 536877553 peak = 1054867456
[M::process] read 675426 sequences (100000156 bp) in 2.046 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286015, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (247.70, 121.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675338 sequences (100000239 bp) in 2.105 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285747, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.79, 122.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675256 sequences (100000201 bp) in 1.030 CPU sec, 1.035 real sec...
[M::mem_process_seqs] Processed 675396 reads in 191.125 CPU sec, 6.136 real sec
[M::mem_process_seqs] Processed 675426 reads in 117.960 CPU sec, 3.794 real sec
sorting block 68, #reads = 1081245 mem = 536878019 peak = 1054867456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285477, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.03, 124.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1080591 mem = 536880607 peak = 1054867456
[M::process] read 675244 sequences (100000149 bp) in 2.718 CPU sec, 3.161 real sec...
[M::process] read 675170 sequences (100000022 bp) in 1.110 CPU sec, 1.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285509, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.09, 124.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 61, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1457)
[M::mem_pestat] mean and std.dev: (166.50, 215.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1941)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675338 reads in 188.533 CPU sec, 6.054 real sec
[M::mem_process_seqs] Processed 675256 reads in 116.278 CPU sec, 3.766 real sec
sorting block 70, #reads = 1080298 mem = 536879234 peak = 1054867456
[M::process] read 675186 sequences (100000107 bp) in 1.698 CPU sec, 1.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285971, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.60, 124.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675188 sequences (100000282 bp) in 1.090 CPU sec, 1.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286011, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.31, 126.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285337, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.58, 127.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675244 reads in 268.347 CPU sec, 8.635 real sec
[M::mem_process_seqs] Processed 675170 reads in 196.793 CPU sec, 6.385 real sec
[M::mem_process_seqs] Processed 675186 reads in 210.668 CPU sec, 6.883 real sec
sorting block 71, #reads = 1080657 mem = 536879137 peak = 1054867456
[M::process] read 675008 sequences (100000218 bp) in 1.910 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286000, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.07, 124.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1080556 mem = 536880965 peak = 1054867456
[M::process] read 675204 sequences (100000124 bp) in 1.849 CPU sec, 2.042 real sec...
[M::process] read 675040 sequences (100000151 bp) in 1.100 CPU sec, 1.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286560, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.88, 125.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675188 reads in 193.201 CPU sec, 6.203 real sec
[M::mem_process_seqs] Processed 675008 reads in 115.678 CPU sec, 3.731 real sec
sorting block 73, #reads = 1080489 mem = 536880835 peak = 1054867456
[M::process] read 674926 sequences (100000039 bp) in 1.879 CPU sec, 2.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286012, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.82, 125.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 293, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333)
[M::mem_pestat] mean and std.dev: (498.08, 501.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4439)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674996 sequences (100000055 bp) in 1.030 CPU sec, 1.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285191, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.29, 127.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 33, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148)
[M::mem_pestat] mean and std.dev: (25.22, 20.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 193)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286050, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.81, 130.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675204 reads in 268.259 CPU sec, 8.631 real sec
[M::mem_process_seqs] Processed 675040 reads in 196.518 CPU sec, 6.382 real sec
[M::mem_process_seqs] Processed 674926 reads in 211.120 CPU sec, 6.904 real sec
sorting block 74, #reads = 1080748 mem = 536877931 peak = 1054867456
[M::process] read 674968 sequences (100000244 bp) in 2.421 CPU sec, 2.837 real sec...
sorting block 75, #reads = 1080469 mem = 536880443 peak = 1054867456
[M::process] read 675000 sequences (100000022 bp) in 2.312 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286102, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.23, 130.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 87, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224)
[M::mem_pestat] mean and std.dev: (210.70, 257.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1631)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674968 reads in 104.144 CPU sec, 3.326 real sec
[M::process] read 675068 sequences (100000275 bp) in 1.493 CPU sec, 1.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285612, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.84, 129.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285572, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.36, 131.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674862 sequences (100000152 bp) in 1.111 CPU sec, 1.121 real sec...
[M::mem_process_seqs] Processed 674996 reads in 297.250 CPU sec, 9.561 real sec
sorting block 76, #reads = 1080635 mem = 536880197 peak = 1054867456
[M::mem_process_seqs] Processed 675000 reads in 133.199 CPU sec, 4.336 real sec
sorting block 77, #reads = 1080215 mem = 536882468 peak = 1086205952
[M::process] read 674954 sequences (100000245 bp) in 1.817 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285780, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.78, 129.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674742 sequences (100000089 bp) in 1.090 CPU sec, 1.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285579, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.52, 130.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285838, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (257.71, 131.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 22, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (95.27, 128.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675068 reads in 270.841 CPU sec, 8.722 real sec
[M::mem_process_seqs] Processed 674862 reads in 196.479 CPU sec, 6.360 real sec
[M::mem_process_seqs] Processed 674954 reads in 211.315 CPU sec, 6.891 real sec
sorting block 78, #reads = 1080895 mem = 536876964 peak = 1086205952
[M::process] read 674838 sequences (100000143 bp) in 2.014 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285434, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.48, 131.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 158, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273)
[M::mem_pestat] mean and std.dev: (214.31, 221.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1696)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 79, #reads = 1080643 mem = 536878116 peak = 1086205952
[M::process] read 674836 sequences (100000143 bp) in 2.324 CPU sec, 2.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285329, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (257.75, 131.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674924 sequences (100000002 bp) in 1.060 CPU sec, 1.057 real sec...
[M::mem_process_seqs] Processed 674742 reads in 195.744 CPU sec, 6.277 real sec
[M::mem_process_seqs] Processed 674838 reads in 118.700 CPU sec, 3.818 real sec
sorting block 80, #reads = 1080125 mem = 536878563 peak = 1086205952
[M::process] read 674790 sequences (100000192 bp) in 1.875 CPU sec, 2.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285452, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (258.16, 132.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 32, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238)
[M::mem_pestat] mean and std.dev: (171.45, 182.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1646)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674666 sequences (100000133 bp) in 1.060 CPU sec, 1.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285886, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.08, 133.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285513, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.85, 133.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674836 reads in 271.156 CPU sec, 8.727 real sec
[M::mem_process_seqs] Processed 674924 reads in 197.154 CPU sec, 6.402 real sec
[M::mem_process_seqs] Processed 674790 reads in 211.670 CPU sec, 6.921 real sec
sorting block 81, #reads = 1080654 mem = 536879388 peak = 1086205952
[M::process] read 674678 sequences (100000272 bp) in 1.845 CPU sec, 2.073 real sec...
sorting block 82, #reads = 1080233 mem = 536877468 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286032, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.92, 133.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674736 sequences (100000218 bp) in 1.600 CPU sec, 1.738 real sec...
[M::process] read 674710 sequences (100000249 bp) in 1.050 CPU sec, 1.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285197, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.56, 134.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 38, 954)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2808)
[M::mem_pestat] mean and std.dev: (472.62, 526.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3735)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674666 reads in 190.988 CPU sec, 6.139 real sec
[M::mem_process_seqs] Processed 674678 reads in 117.070 CPU sec, 3.790 real sec
sorting block 83, #reads = 1080686 mem = 536881619 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285365, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.44, 134.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 273, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (216.91, 139.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1392)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674580 sequences (100000268 bp) in 2.485 CPU sec, 2.894 real sec...
[M::process] read 674690 sequences (100000076 bp) in 1.030 CPU sec, 1.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285455, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.51, 135.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674736 reads in 187.741 CPU sec, 6.026 real sec
[M::mem_process_seqs] Processed 674710 reads in 115.385 CPU sec, 3.748 real sec
sorting block 84, #reads = 1080417 mem = 536879652 peak = 1086205952
[M::process] read 674506 sequences (100000067 bp) in 1.631 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285664, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.68, 135.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674532 sequences (100000225 bp) in 1.080 CPU sec, 1.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285478, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.00, 137.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285452, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.46, 135.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674580 reads in 267.196 CPU sec, 8.603 real sec
[M::mem_process_seqs] Processed 674506 reads in 195.514 CPU sec, 6.351 real sec
sorting block 85, #reads = 1079796 mem = 536878290 peak = 1086205952
[M::mem_process_seqs] Processed 674690 reads in 209.351 CPU sec, 6.845 real sec
sorting block 86, #reads = 1079738 mem = 536878898 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285178, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.93, 138.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 15, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231)
[M::mem_pestat] mean and std.dev: (170.90, 257.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1641)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674488 sequences (100000255 bp) in 2.740 CPU sec, 3.197 real sec...
[M::mem_process_seqs] Processed 674532 reads in 101.400 CPU sec, 3.386 real sec
[M::process] read 674522 sequences (100000139 bp) in 2.403 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285938, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.22, 139.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 215, 2031)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5891)
[M::mem_pestat] mean and std.dev: (859.15, 1031.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7821)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 87, #reads = 1079775 mem = 536880635 peak = 1086205952
[M::process] read 674526 sequences (100000174 bp) in 1.661 CPU sec, 1.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285303, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (267.01, 139.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675932 sequences (100000070 bp) in 1.070 CPU sec, 1.070 real sec...
[M::mem_process_seqs] Processed 674488 reads in 186.779 CPU sec, 5.990 real sec
[M::mem_process_seqs] Processed 674522 reads in 116.033 CPU sec, 3.732 real sec
sorting block 88, #reads = 1079887 mem = 536881334 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285521, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (267.80, 139.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675828 sequences (100000199 bp) in 2.684 CPU sec, 3.108 real sec...
[M::process] read 675684 sequences (100000247 bp) in 1.130 CPU sec, 1.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286891, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (237.30, 112.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674526 reads in 182.431 CPU sec, 5.852 real sec
[M::mem_process_seqs] Processed 675932 reads in 111.564 CPU sec, 3.608 real sec
sorting block 89, #reads = 1079564 mem = 536877671 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286690, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (239.03, 114.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675678 sequences (100000000 bp) in 2.043 CPU sec, 2.328 real sec...
[M::process] read 675636 sequences (100000024 bp) in 1.150 CPU sec, 1.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286817, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (240.24, 115.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675828 reads in 180.542 CPU sec, 5.799 real sec
sorting block 90, #reads = 1080468 mem = 536879334 peak = 1086205952
[M::mem_process_seqs] Processed 675684 reads in 111.190 CPU sec, 3.610 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286425, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (241.08, 115.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 288, 1555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4573)
[M::mem_pestat] mean and std.dev: (604.31, 659.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6082)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 91, #reads = 1080903 mem = 536877558 peak = 1086205952
[M::process] read 675734 sequences (100000063 bp) in 2.582 CPU sec, 2.899 real sec...
[M::process] read 675712 sequences (100000072 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286582, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.73, 116.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675678 reads in 183.643 CPU sec, 5.881 real sec
[M::mem_process_seqs] Processed 675636 reads in 114.435 CPU sec, 3.700 real sec
sorting block 92, #reads = 1081051 mem = 536882801 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286498, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.97, 117.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675512 sequences (100000062 bp) in 2.561 CPU sec, 2.817 real sec...
[M::process] read 675380 sequences (100000267 bp) in 1.140 CPU sec, 1.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285953, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.98, 117.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675734 reads in 184.869 CPU sec, 5.931 real sec
[M::mem_process_seqs] Processed 675712 reads in 113.069 CPU sec, 3.654 real sec
sorting block 93, #reads = 1081325 mem = 536878560 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286294, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.78, 118.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675596 sequences (100000139 bp) in 2.102 CPU sec, 2.298 real sec...
[M::process] read 675488 sequences (100000255 bp) in 1.510 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286200, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.48, 118.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675512 reads in 183.210 CPU sec, 5.873 real sec
[M::mem_process_seqs] Processed 675380 reads in 112.215 CPU sec, 3.634 real sec
sorting block 94, #reads = 1080289 mem = 536879890 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286225, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (243.17, 117.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675430 sequences (100000263 bp) in 2.368 CPU sec, 2.627 real sec...
[M::process] read 674600 sequences (100000134 bp) in 1.190 CPU sec, 1.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286290, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (245.06, 119.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675596 reads in 183.699 CPU sec, 5.899 real sec
sorting block 95, #reads = 1080485 mem = 536879413 peak = 1086205952
[M::mem_process_seqs] Processed 675488 reads in 113.622 CPU sec, 3.675 real sec
sorting block 96, #reads = 1080844 mem = 536882751 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286156, 16, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (246.26, 120.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (57, 73, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497)
[M::mem_pestat] mean and std.dev: (193.93, 239.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1977)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674844 sequences (100000160 bp) in 2.451 CPU sec, 2.772 real sec...
[M::process] read 675258 sequences (100000138 bp) in 1.160 CPU sec, 1.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285693, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.10, 133.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675430 reads in 184.863 CPU sec, 5.934 real sec
[M::mem_process_seqs] Processed 674600 reads in 113.525 CPU sec, 3.677 real sec
sorting block 97, #reads = 1080641 mem = 536877945 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285422, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.35, 129.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675328 sequences (100000219 bp) in 2.293 CPU sec, 2.634 real sec...
[M::process] read 675318 sequences (100000038 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285786, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (246.79, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674844 reads in 186.326 CPU sec, 5.969 real sec
[M::mem_process_seqs] Processed 675258 reads in 115.076 CPU sec, 3.723 real sec
sorting block 98, #reads = 1079799 mem = 536878091 peak = 1086205952
[M::process] read 675112 sequences (100000175 bp) in 2.058 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286050, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (247.03, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 100, 108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (75.91, 51.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 366)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675184 sequences (100000109 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285773, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.70, 122.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286234, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.93, 123.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 380, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435)
[M::mem_pestat] mean and std.dev: (238.75, 222.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1910)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675328 reads in 269.577 CPU sec, 8.666 real sec
[M::mem_process_seqs] Processed 675318 reads in 197.053 CPU sec, 6.381 real sec
sorting block 99, #reads = 1080282 mem = 536879626 peak = 1086205952
[M::mem_process_seqs] Processed 675112 reads in 211.783 CPU sec, 6.908 real sec
sorting block 100, #reads = 1080965 mem = 536879233 peak = 1086205952
[M::process] read 674970 sequences (100000167 bp) in 1.965 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285709, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.81, 124.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675090 sequences (100000139 bp) in 1.902 CPU sec, 2.071 real sec...
[M::process] read 675018 sequences (100000152 bp) in 1.110 CPU sec, 1.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286001, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (252.28, 125.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 41, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (41.46, 57.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675184 reads in 190.770 CPU sec, 6.128 real sec
[M::mem_process_seqs] Processed 674970 reads in 117.437 CPU sec, 3.786 real sec
sorting block 101, #reads = 1080103 mem = 536880656 peak = 1086205952
[M::process] read 675056 sequences (100000234 bp) in 1.917 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285483, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.75, 125.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675168 sequences (100000219 bp) in 1.240 CPU sec, 1.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285456, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.75, 124.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285913, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (251.13, 124.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675090 reads in 270.829 CPU sec, 8.710 real sec
sorting block 102, #reads = 1080402 mem = 536879814 peak = 1086205952
[M::mem_process_seqs] Processed 675018 reads in 197.681 CPU sec, 6.407 real sec
[M::mem_process_seqs] Processed 675056 reads in 211.570 CPU sec, 6.904 real sec
sorting block 103, #reads = 1080436 mem = 536880929 peak = 1086205952
[M::process] read 674580 sequences (100000271 bp) in 2.052 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285493, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.43, 124.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674946 sequences (100000270 bp) in 2.522 CPU sec, 2.853 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285520, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.26, 133.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675168 reads in 191.978 CPU sec, 6.163 real sec
[M::process] read 675006 sequences (100000176 bp) in 1.212 CPU sec, 1.229 real sec...
[M::mem_process_seqs] Processed 674580 reads in 119.771 CPU sec, 3.853 real sec
sorting block 104, #reads = 1080307 mem = 536878652 peak = 1086205952
[M::process] read 674996 sequences (100000041 bp) in 1.894 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285888, 18, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (255.10, 128.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 30, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (42.73, 62.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675000 sequences (100000134 bp) in 1.050 CPU sec, 1.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285926, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.92, 125.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285791, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (253.19, 125.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 159, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (197.58, 208.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1412)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674946 reads in 268.564 CPU sec, 8.647 real sec
sorting block 105, #reads = 1079893 mem = 536879548 peak = 1086205952
[M::mem_process_seqs] Processed 675006 reads in 195.399 CPU sec, 6.338 real sec
[M::mem_process_seqs] Processed 674996 reads in 209.304 CPU sec, 6.836 real sec
sorting block 106, #reads = 1080801 mem = 536877570 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285819, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.93, 128.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674946 sequences (100000276 bp) in 2.494 CPU sec, 2.884 real sec...
[M::mem_process_seqs] Processed 675000 reads in 102.675 CPU sec, 3.416 real sec
sorting block 107, #reads = 1080446 mem = 536880615 peak = 1086205952
[M::process] read 674936 sequences (100000237 bp) in 1.871 CPU sec, 2.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285847, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.74, 128.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 537, 1586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4586)
[M::mem_pestat] mean and std.dev: (569.67, 625.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6086)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674914 sequences (100000023 bp) in 1.456 CPU sec, 1.514 real sec...
[M::process] read 674344 sequences (100000188 bp) in 1.110 CPU sec, 1.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285909, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (255.40, 128.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674946 reads in 187.811 CPU sec, 6.022 real sec
[M::mem_process_seqs] Processed 674936 reads in 115.716 CPU sec, 3.751 real sec
sorting block 108, #reads = 1080318 mem = 536879596 peak = 1086205952
[M::process] read 674734 sequences (100000256 bp) in 2.005 CPU sec, 2.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286296, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.81, 129.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 37, 117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303)
[M::mem_pestat] mean and std.dev: (35.44, 30.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 396)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674898 sequences (100000265 bp) in 1.006 CPU sec, 1.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284786, 28, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.73, 136.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 13, 175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (63.19, 100.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285427, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.76, 132.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674914 reads in 271.435 CPU sec, 8.734 real sec
sorting block 109, #reads = 1080757 mem = 536882234 peak = 1086205952
[M::mem_process_seqs] Processed 674344 reads in 198.209 CPU sec, 6.427 real sec
[M::mem_process_seqs] Processed 674734 reads in 212.069 CPU sec, 6.921 real sec
sorting block 110, #reads = 1079690 mem = 536879903 peak = 1086205952
[M::process] read 674820 sequences (100000197 bp) in 1.790 CPU sec, 1.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285783, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (258.90, 132.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 17, 32)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 94)
[M::mem_pestat] mean and std.dev: (13.87, 19.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 125)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674682 sequences (100000085 bp) in 2.301 CPU sec, 2.626 real sec...
[M::process] read 674740 sequences (100000232 bp) in 1.040 CPU sec, 1.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285865, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.39, 132.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 376, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (228.00, 202.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1462)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674898 reads in 192.868 CPU sec, 6.182 real sec
[M::mem_process_seqs] Processed 674820 reads in 115.818 CPU sec, 3.732 real sec
sorting block 111, #reads = 1080182 mem = 536880927 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285782, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.21, 133.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674770 sequences (100000172 bp) in 2.444 CPU sec, 2.834 real sec...
[M::process] read 674444 sequences (100000122 bp) in 1.050 CPU sec, 1.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286085, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.85, 134.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 223, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (202.70, 156.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674682 reads in 184.291 CPU sec, 5.914 real sec
sorting block 112, #reads = 1080286 mem = 536878502 peak = 1086205952
[M::mem_process_seqs] Processed 674740 reads in 113.701 CPU sec, 3.689 real sec
sorting block 113, #reads = 1080770 mem = 536879740 peak = 1086205952
[M::process] read 674428 sequences (100000223 bp) in 1.729 CPU sec, 1.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285779, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.79, 134.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 73, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384)
[M::mem_pestat] mean and std.dev: (168.83, 189.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1837)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674618 sequences (100000066 bp) in 1.090 CPU sec, 1.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285113, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.09, 139.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (76, 76, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1888)
[M::mem_pestat] mean and std.dev: (267.18, 295.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2492)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285607, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (266.82, 138.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674770 reads in 268.362 CPU sec, 8.639 real sec
[M::mem_process_seqs] Processed 674444 reads in 196.484 CPU sec, 6.377 real sec
[M::mem_process_seqs] Processed 674428 reads in 210.929 CPU sec, 6.891 real sec
sorting block 114, #reads = 1080159 mem = 536879828 peak = 1086205952
[M::process] read 674064 sequences (100000235 bp) in 1.960 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285205, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.97, 134.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1080209 mem = 536879657 peak = 1086205952
[M::process] read 674714 sequences (100000095 bp) in 2.028 CPU sec, 2.313 real sec...
[M::process] read 674690 sequences (100000056 bp) in 1.090 CPU sec, 1.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284755, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.40, 141.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 190, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1788)
[M::mem_pestat] mean and std.dev: (273.71, 248.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2357)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674618 reads in 193.489 CPU sec, 6.212 real sec
[M::mem_process_seqs] Processed 674064 reads in 116.742 CPU sec, 3.765 real sec
sorting block 116, #reads = 1080351 mem = 536878243 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286098, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.34, 133.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674526 sequences (100000187 bp) in 2.006 CPU sec, 2.226 real sec...
[M::process] read 674490 sequences (100000245 bp) in 1.040 CPU sec, 1.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286104, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.01, 134.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674714 reads in 183.352 CPU sec, 5.896 real sec
[M::mem_process_seqs] Processed 674690 reads in 111.522 CPU sec, 3.627 real sec
sorting block 117, #reads = 1079985 mem = 536878883 peak = 1086205952
[M::process] read 674364 sequences (100000027 bp) in 1.586 CPU sec, 1.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285820, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.41, 136.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (309, 313, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (143, 558)
[M::mem_pestat] mean and std.dev: (335.78, 40.09)
[M::mem_pestat] low and high boundaries for proper pairs: (60, 641)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675494 sequences (100000181 bp) in 1.050 CPU sec, 1.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285480, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.94, 136.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 53, 63)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183)
[M::mem_pestat] mean and std.dev: (30.85, 26.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 243)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285955, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.73, 139.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 66, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (118.36, 142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674526 reads in 260.175 CPU sec, 8.380 real sec
[M::mem_process_seqs] Processed 674490 reads in 189.446 CPU sec, 6.151 real sec
sorting block 118, #reads = 1080244 mem = 536880880 peak = 1086205952
[M::mem_process_seqs] Processed 674364 reads in 204.448 CPU sec, 6.688 real sec
[M::process] read 676302 sequences (100000030 bp) in 1.904 CPU sec, 2.103 real sec...
sorting block 119, #reads = 1079490 mem = 536877471 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286318, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.36, 124.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 32, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (95.58, 118.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676152 sequences (100000109 bp) in 1.664 CPU sec, 1.798 real sec...
[M::process] read 675938 sequences (100000280 bp) in 1.070 CPU sec, 1.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287046, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (236.57, 115.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675494 reads in 188.499 CPU sec, 6.065 real sec
[M::mem_process_seqs] Processed 676302 reads in 113.919 CPU sec, 3.680 real sec
sorting block 120, #reads = 1080467 mem = 536876277 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286699, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.52, 116.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 98, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (139.50, 120.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676036 sequences (100000165 bp) in 2.505 CPU sec, 2.925 real sec...
[M::process] read 675904 sequences (100000242 bp) in 1.040 CPU sec, 1.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286049, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (239.88, 117.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676152 reads in 184.470 CPU sec, 5.921 real sec
[M::mem_process_seqs] Processed 675938 reads in 113.632 CPU sec, 3.680 real sec
sorting block 121, #reads = 1081460 mem = 536879776 peak = 1086205952
[M::process] read 675718 sequences (100000180 bp) in 1.673 CPU sec, 1.846 real sec...
[M::process] read 675882 sequences (100000278 bp) in 1.050 CPU sec, 1.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286319, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.05, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675718 reads in 102.048 CPU sec, 3.297 real sec
sorting block 122, #reads = 1080996 mem = 536878682 peak = 1086205952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286321, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (240.78, 118.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675908 sequences (100000059 bp) in 1.101 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286447, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.05, 117.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676036 reads in 285.969 CPU sec, 9.206 real sec
[M::mem_process_seqs] Processed 675904 reads in 214.572 CPU sec, 6.960 real sec
sorting block 123, #reads = 1081238 mem = 536879822 peak = 1109897216
[M::process] read 675870 sequences (100000003 bp) in 1.652 CPU sec, 1.829 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286352, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.05, 119.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 11, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (151.80, 184.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1439)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675828 sequences (100000027 bp) in 1.010 CPU sec, 1.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286328, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (241.72, 120.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285598, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.73, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 128, 221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (81.56, 81.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675882 reads in 268.324 CPU sec, 8.640 real sec
sorting block 124, #reads = 1081104 mem = 536883766 peak = 1109897216
[M::mem_process_seqs] Processed 675908 reads in 197.278 CPU sec, 6.409 real sec
[M::mem_process_seqs] Processed 675870 reads in 211.843 CPU sec, 6.930 real sec
sorting block 125, #reads = 1081599 mem = 536880791 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285689, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675852 sequences (100000052 bp) in 2.470 CPU sec, 2.914 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.16, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675602 sequences (100000033 bp) in 2.272 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285974, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.17, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675828 reads in 192.899 CPU sec, 6.255 real sec
[M::mem_process_seqs] Processed 675852 reads in 120.363 CPU sec, 3.866 real sec
[M::process] read 675694 sequences (100000122 bp) in 1.547 CPU sec, 1.665 real sec...
sorting block 126, #reads = 1080824 mem = 536880008 peak = 1109897216
sorting block 127, #reads = 1080910 mem = 536880580 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285532, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.00, 122.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675558 sequences (100000104 bp) in 1.894 CPU sec, 2.074 real sec...
[M::process] read 675466 sequences (100000286 bp) in 1.080 CPU sec, 1.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285739, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.37, 121.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675602 reads in 190.574 CPU sec, 6.132 real sec
[M::mem_process_seqs] Processed 675694 reads in 115.992 CPU sec, 3.767 real sec
sorting block 128, #reads = 1081017 mem = 536878363 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285649, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.96, 123.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675546 sequences (100000112 bp) in 2.225 CPU sec, 2.554 real sec...
[M::process] read 675500 sequences (100000131 bp) in 1.060 CPU sec, 1.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285868, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.26, 123.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675558 reads in 190.994 CPU sec, 6.135 real sec
[M::mem_process_seqs] Processed 675466 reads in 115.807 CPU sec, 3.754 real sec
sorting block 129, #reads = 1080872 mem = 536880857 peak = 1109897216
[M::process] read 675384 sequences (100000074 bp) in 2.024 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285687, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.68, 123.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 194, 2561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7639)
[M::mem_pestat] mean and std.dev: (941.25, 1162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10178)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675456 sequences (100000085 bp) in 1.080 CPU sec, 1.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285525, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.77, 125.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675546 reads in 188.892 CPU sec, 6.069 real sec
[M::mem_process_seqs] Processed 675500 reads in 115.116 CPU sec, 3.738 real sec
sorting block 130, #reads = 1080581 mem = 536878573 peak = 1109897216
[M::process] read 675430 sequences (100000018 bp) in 1.849 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285652, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.38, 126.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675396 sequences (100000253 bp) in 1.060 CPU sec, 1.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285773, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.33, 126.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675384 reads in 191.018 CPU sec, 6.146 real sec
[M::mem_process_seqs] Processed 675456 reads in 116.140 CPU sec, 3.774 real sec
sorting block 131, #reads = 1080765 mem = 536877750 peak = 1109897216
sorting block 132, #reads = 1080991 mem = 536878049 peak = 1109897216
[M::process] read 675390 sequences (100000058 bp) in 1.873 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285486, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.47, 128.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675148 sequences (100000130 bp) in 1.060 CPU sec, 1.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285322, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.69, 126.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286046, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.31, 127.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675430 reads in 275.126 CPU sec, 8.863 real sec
[M::mem_process_seqs] Processed 675396 reads in 200.540 CPU sec, 6.501 real sec
[M::mem_process_seqs] Processed 675390 reads in 214.309 CPU sec, 6.995 real sec
sorting block 133, #reads = 1081008 mem = 536879765 peak = 1109897216
[M::process] read 675270 sequences (100000207 bp) in 1.884 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285483, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.94, 126.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675298 sequences (100000228 bp) in 1.605 CPU sec, 1.804 real sec...
[M::process] read 675156 sequences (100000243 bp) in 1.050 CPU sec, 1.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285289, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.51, 128.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 166, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 226)
[M::mem_pestat] mean and std.dev: (176.00, 10.00)
[M::mem_pestat] low and high boundaries for proper pairs: (106, 246)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675148 reads in 196.967 CPU sec, 6.335 real sec
sorting block 134, #reads = 1080933 mem = 536879908 peak = 1109897216
[M::mem_process_seqs] Processed 675270 reads in 116.635 CPU sec, 3.775 real sec
sorting block 135, #reads = 1080990 mem = 536878177 peak = 1109897216
[M::process] read 675036 sequences (100000132 bp) in 2.681 CPU sec, 3.161 real sec...
[M::process] read 674978 sequences (100000187 bp) in 1.050 CPU sec, 1.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285599, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.07, 127.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675156 reads in 103.177 CPU sec, 3.312 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285464, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (251.82, 128.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 26, 644)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1924)
[M::mem_pestat] mean and std.dev: (220.62, 314.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2564)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675136 sequences (100000024 bp) in 1.179 CPU sec, 1.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285586, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.58, 128.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675298 reads in 292.153 CPU sec, 9.386 real sec
sorting block 136, #reads = 1080650 mem = 536877217 peak = 1109897216
[M::mem_process_seqs] Processed 675036 reads in 131.240 CPU sec, 4.290 real sec
sorting block 137, #reads = 1079513 mem = 536879511 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285327, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (254.09, 129.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674946 sequences (100000010 bp) in 1.896 CPU sec, 2.159 real sec...
[M::process] read 675112 sequences (100000228 bp) in 1.110 CPU sec, 1.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285424, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.84, 128.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 34, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (148.09, 168.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674978 reads in 188.845 CPU sec, 6.079 real sec
[M::mem_process_seqs] Processed 675136 reads in 115.780 CPU sec, 3.763 real sec
sorting block 138, #reads = 1080066 mem = 536879387 peak = 1109897216
[M::process] read 675040 sequences (100000041 bp) in 1.577 CPU sec, 1.695 real sec...
[M::process] read 675062 sequences (100000007 bp) in 1.020 CPU sec, 1.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285324, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.86, 130.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 128, 157)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333)
[M::mem_pestat] mean and std.dev: (92.67, 62.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675040 reads in 102.365 CPU sec, 3.311 real sec
sorting block 139, #reads = 1080932 mem = 536879061 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285654, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.94, 129.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674880 sequences (100000135 bp) in 1.083 CPU sec, 1.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285480, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.51, 128.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 73, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 81)
[M::mem_pestat] mean and std.dev: (72.33, 1.49)
[M::mem_pestat] low and high boundaries for proper pairs: (57, 85)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674946 reads in 287.865 CPU sec, 9.275 real sec
[M::mem_process_seqs] Processed 675112 reads in 215.309 CPU sec, 6.987 real sec
sorting block 140, #reads = 1080497 mem = 536876742 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285315, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.51, 129.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 47, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266)
[M::mem_pestat] mean and std.dev: (60.08, 68.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 350)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675070 sequences (100000290 bp) in 2.088 CPU sec, 2.431 real sec...
[M::process] read 675082 sequences (100000278 bp) in 1.070 CPU sec, 1.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285571, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (256.22, 131.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675062 reads in 191.500 CPU sec, 6.153 real sec
[M::mem_process_seqs] Processed 674880 reads in 116.142 CPU sec, 3.773 real sec
sorting block 141, #reads = 1080563 mem = 536877795 peak = 1109897216
[M::process] read 675022 sequences (100000234 bp) in 1.619 CPU sec, 1.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285892, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.75, 131.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674810 sequences (100000296 bp) in 1.010 CPU sec, 1.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285366, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.02, 132.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 73, 108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318)
[M::mem_pestat] mean and std.dev: (59.78, 47.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285338, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.49, 133.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675070 reads in 273.112 CPU sec, 8.796 real sec
[M::mem_process_seqs] Processed 675082 reads in 199.115 CPU sec, 6.464 real sec
[M::mem_process_seqs] Processed 675022 reads in 213.609 CPU sec, 6.983 real sec
sorting block 142, #reads = 1080063 mem = 536878538 peak = 1109897216
[M::process] read 674958 sequences (100000079 bp) in 2.397 CPU sec, 2.785 real sec...
sorting block 143, #reads = 1080836 mem = 536878837 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285464, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.97, 133.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 26, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (101.54, 146.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 741)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674918 sequences (100000128 bp) in 1.544 CPU sec, 1.670 real sec...
[M::process] read 675052 sequences (100000124 bp) in 1.070 CPU sec, 1.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285412, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.79, 133.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 172, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831)
[M::mem_pestat] mean and std.dev: (294.50, 285.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2433)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674810 reads in 195.572 CPU sec, 6.301 real sec
[M::mem_process_seqs] Processed 674958 reads in 119.426 CPU sec, 3.854 real sec
sorting block 144, #reads = 1079818 mem = 536878312 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285805, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.46, 134.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 46, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (90.00, 133.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674814 sequences (100000021 bp) in 2.458 CPU sec, 2.850 real sec...
[M::process] read 674852 sequences (100000158 bp) in 1.050 CPU sec, 1.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285617, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.54, 134.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674918 reads in 190.103 CPU sec, 6.103 real sec
[M::mem_process_seqs] Processed 675052 reads in 115.788 CPU sec, 3.755 real sec
sorting block 145, #reads = 1080596 mem = 536877819 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285488, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.41, 135.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674862 sequences (100000132 bp) in 2.057 CPU sec, 2.364 real sec...
[M::process] read 674866 sequences (100000184 bp) in 1.060 CPU sec, 1.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285822, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (261.09, 136.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674814 reads in 187.577 CPU sec, 6.025 real sec
sorting block 146, #reads = 1080415 mem = 536880178 peak = 1109897216
[M::mem_process_seqs] Processed 674852 reads in 114.721 CPU sec, 3.732 real sec
sorting block 147, #reads = 1080484 mem = 536879552 peak = 1109897216
[M::process] read 675188 sequences (100000088 bp) in 1.962 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285694, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.89, 135.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 448, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (386.59, 208.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676304 sequences (100000239 bp) in 1.110 CPU sec, 1.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285941, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (262.00, 138.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (445, 574, 1542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3736)
[M::mem_pestat] mean and std.dev: (581.88, 405.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4833)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674862 reads in 186.432 CPU sec, 5.992 real sec
[M::mem_process_seqs] Processed 674866 reads in 115.026 CPU sec, 3.739 real sec
sorting block 148, #reads = 1080523 mem = 536878162 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285904, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (254.39, 132.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676212 sequences (100000267 bp) in 2.156 CPU sec, 2.487 real sec...
[M::process] read 675924 sequences (100000167 bp) in 1.050 CPU sec, 1.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286835, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (235.82, 113.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675188 reads in 182.752 CPU sec, 5.867 real sec
[M::mem_process_seqs] Processed 676304 reads in 110.838 CPU sec, 3.598 real sec
sorting block 149, #reads = 1080280 mem = 536877614 peak = 1109897216
[M::process] read 675968 sequences (100000106 bp) in 1.729 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287207, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (238.23, 115.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 20, 117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335)
[M::mem_pestat] mean and std.dev: (24.62, 35.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 444)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675880 sequences (100000071 bp) in 1.050 CPU sec, 1.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286669, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.73, 116.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 11, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1810)
[M::mem_pestat] mean and std.dev: (275.31, 358.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2412)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286190, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (240.50, 118.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676212 reads in 260.887 CPU sec, 8.393 real sec
[M::mem_process_seqs] Processed 675924 reads in 191.795 CPU sec, 6.218 real sec
sorting block 150, #reads = 1081566 mem = 536880757 peak = 1109897216
[M::mem_process_seqs] Processed 675968 reads in 206.506 CPU sec, 6.744 real sec
sorting block 151, #reads = 1081198 mem = 536878850 peak = 1109897216
[M::process] read 675906 sequences (100000149 bp) in 2.261 CPU sec, 2.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286584, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (241.38, 118.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675752 sequences (100000041 bp) in 1.651 CPU sec, 1.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286554, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (242.23, 119.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675746 sequences (100000180 bp) in 1.080 CPU sec, 1.082 real sec...
[M::mem_process_seqs] Processed 675880 reads in 185.828 CPU sec, 5.979 real sec
[M::mem_process_seqs] Processed 675906 reads in 115.616 CPU sec, 3.718 real sec
sorting block 152, #reads = 1080846 mem = 536880513 peak = 1109897216
[M::process] read 675642 sequences (100000156 bp) in 1.842 CPU sec, 2.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286630, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.16, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675712 sequences (100000272 bp) in 1.040 CPU sec, 1.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286080, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (244.44, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675752 reads in 186.100 CPU sec, 5.992 real sec
sorting block 153, #reads = 1080961 mem = 536879168 peak = 1109897216
[M::mem_process_seqs] Processed 675746 reads in 115.126 CPU sec, 3.744 real sec
sorting block 154, #reads = 1081143 mem = 536877542 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286210, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.89, 120.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675632 sequences (100000088 bp) in 2.075 CPU sec, 2.415 real sec...
[M::process] read 675606 sequences (100000284 bp) in 1.130 CPU sec, 1.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286536, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.71, 121.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675642 reads in 186.248 CPU sec, 5.984 real sec
[M::mem_process_seqs] Processed 675712 reads in 114.265 CPU sec, 3.717 real sec
sorting block 155, #reads = 1080711 mem = 536880089 peak = 1109897216
[M::process] read 675708 sequences (100000132 bp) in 1.796 CPU sec, 1.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285834, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.98, 121.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675604 sequences (100000024 bp) in 1.060 CPU sec, 1.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285625, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.37, 122.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286125, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.86, 123.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675632 reads in 271.664 CPU sec, 8.752 real sec
[M::mem_process_seqs] Processed 675606 reads in 199.059 CPU sec, 6.455 real sec
[M::mem_process_seqs] Processed 675708 reads in 213.955 CPU sec, 6.987 real sec
sorting block 156, #reads = 1080827 mem = 536879465 peak = 1109897216
[M::process] read 675540 sequences (100000072 bp) in 1.979 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285980, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.15, 121.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 11, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215)
[M::mem_pestat] mean and std.dev: (24.00, 24.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 157, #reads = 1080571 mem = 536879484 peak = 1109897216
[M::process] read 675570 sequences (100000223 bp) in 2.167 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285678, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.53, 123.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675326 sequences (100000003 bp) in 1.070 CPU sec, 1.070 real sec...
[M::mem_process_seqs] Processed 675604 reads in 193.913 CPU sec, 6.223 real sec
[M::mem_process_seqs] Processed 675540 reads in 118.857 CPU sec, 3.824 real sec
sorting block 158, #reads = 1081089 mem = 536878800 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285991, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (247.05, 123.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675516 sequences (100000130 bp) in 2.706 CPU sec, 3.222 real sec...
[M::process] read 675422 sequences (100000133 bp) in 1.070 CPU sec, 1.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286149, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.33, 124.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 32, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2135)
[M::mem_pestat] mean and std.dev: (224.55, 313.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2841)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675570 reads in 188.861 CPU sec, 6.060 real sec
[M::mem_process_seqs] Processed 675326 reads in 114.235 CPU sec, 3.693 real sec
sorting block 159, #reads = 1080747 mem = 536882287 peak = 1109897216
[M::process] read 675492 sequences (100000256 bp) in 2.037 CPU sec, 2.336 real sec...
[M::process] read 675518 sequences (100000141 bp) in 1.080 CPU sec, 1.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285419, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (249.10, 126.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675492 reads in 98.698 CPU sec, 3.246 real sec
sorting block 160, #reads = 1079775 mem = 536879251 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285592, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (248.89, 126.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 406, 3977)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11881)
[M::mem_pestat] mean and std.dev: (1787.71, 2380.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15833)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675422 reads in 202.256 CPU sec, 7.125 real sec
sorting block 161, #reads = 1080816 mem = 536881580 peak = 1109897216
[M::process] read 675536 sequences (100000110 bp) in 2.162 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285588, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (248.59, 126.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (697, 779, 900)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (291, 1306)
[M::mem_pestat] mean and std.dev: (778.43, 84.05)
[M::mem_pestat] low and high boundaries for proper pairs: (88, 1509)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286072, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (248.03, 125.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 100, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301)
[M::mem_pestat] mean and std.dev: (193.50, 229.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1731)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675450 sequences (100000144 bp) in 1.016 CPU sec, 1.019 real sec...
[M::mem_process_seqs] Processed 675516 reads in 395.306 CPU sec, 13.297 real sec
[M::mem_process_seqs] Processed 675518 reads in 135.450 CPU sec, 4.382 real sec
sorting block 162, #reads = 1080605 mem = 536881897 peak = 1109897216
[M::process] read 675248 sequences (100000175 bp) in 1.787 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285931, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.05, 125.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675186 sequences (100000215 bp) in 1.060 CPU sec, 1.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285933, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.62, 125.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 267, 1690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5020)
[M::mem_pestat] mean and std.dev: (724.87, 796.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6685)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284934, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (250.71, 127.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 100, 100)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236)
[M::mem_pestat] mean and std.dev: (70.25, 56.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 304)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675536 reads in 273.224 CPU sec, 8.806 real sec
[M::mem_process_seqs] Processed 675450 reads in 199.092 CPU sec, 6.453 real sec
[M::mem_process_seqs] Processed 675248 reads in 213.128 CPU sec, 6.956 real sec
sorting block 163, #reads = 1080753 mem = 536883012 peak = 1109897216
[M::process] read 675198 sequences (100000016 bp) in 2.375 CPU sec, 2.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285180, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.79, 128.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 164, #reads = 1078207 mem = 536876917 peak = 1109897216
[M::process] read 675296 sequences (100000158 bp) in 1.626 CPU sec, 1.770 real sec...
[M::process] read 675104 sequences (100000200 bp) in 1.050 CPU sec, 1.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285066, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.09, 128.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675186 reads in 198.362 CPU sec, 6.393 real sec
[M::mem_process_seqs] Processed 675198 reads in 120.321 CPU sec, 3.882 real sec
sorting block 165, #reads = 1080187 mem = 536880786 peak = 1109897216
[M::process] read 675248 sequences (100000093 bp) in 1.847 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285374, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.20, 129.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675148 sequences (100000085 bp) in 1.080 CPU sec, 1.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285233, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.72, 130.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 104, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1483)
[M::mem_pestat] mean and std.dev: (136.40, 174.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1971)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285681, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.70, 129.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675296 reads in 276.064 CPU sec, 8.890 real sec
[M::mem_process_seqs] Processed 675104 reads in 201.611 CPU sec, 6.534 real sec
sorting block 166, #reads = 1079977 mem = 536879145 peak = 1109897216
[M::mem_process_seqs] Processed 675248 reads in 216.280 CPU sec, 7.069 real sec
[M::process] read 674982 sequences (100000203 bp) in 1.923 CPU sec, 2.169 real sec...
sorting block 167, #reads = 1080540 mem = 536877805 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285599, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.82, 129.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 272, 923)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2739)
[M::mem_pestat] mean and std.dev: (439.00, 435.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3647)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675014 sequences (100000286 bp) in 2.351 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285518, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.47, 131.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674898 sequences (100000013 bp) in 1.070 CPU sec, 1.063 real sec...
[M::mem_process_seqs] Processed 675148 reads in 199.836 CPU sec, 6.409 real sec
[M::mem_process_seqs] Processed 674982 reads in 119.604 CPU sec, 3.857 real sec
sorting block 168, #reads = 1079250 mem = 536879471 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285500, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.42, 131.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674994 sequences (100000232 bp) in 2.576 CPU sec, 3.005 real sec...
[M::process] read 675034 sequences (100000167 bp) in 1.220 CPU sec, 1.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285658, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.57, 131.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675014 reads in 192.654 CPU sec, 6.181 real sec
[M::mem_process_seqs] Processed 674898 reads in 117.746 CPU sec, 3.809 real sec
sorting block 169, #reads = 1080736 mem = 536879002 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285078, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.40, 132.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 19, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148)
[M::mem_pestat] mean and std.dev: (27.30, 20.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 193)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675114 sequences (100000071 bp) in 2.201 CPU sec, 2.526 real sec...
[M::process] read 674980 sequences (100000137 bp) in 1.260 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285351, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.50, 132.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 2218, 4300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12508)
[M::mem_pestat] mean and std.dev: (2202.23, 1653.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16612)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674994 reads in 195.634 CPU sec, 6.284 real sec
sorting block 170, #reads = 1080345 mem = 536879935 peak = 1109897216
[M::mem_process_seqs] Processed 675034 reads in 117.453 CPU sec, 3.809 real sec
sorting block 171, #reads = 1080565 mem = 536878724 peak = 1109897216
[M::process] read 675028 sequences (100000085 bp) in 2.133 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285106, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.38, 132.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 73, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1726)
[M::mem_pestat] mean and std.dev: (337.88, 490.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2299)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674868 sequences (100000070 bp) in 1.486 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285440, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.31, 133.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284932, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.21, 134.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 38, 152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380)
[M::mem_pestat] mean and std.dev: (49.56, 44.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675114 reads in 280.288 CPU sec, 9.004 real sec
[M::mem_process_seqs] Processed 674980 reads in 203.787 CPU sec, 6.591 real sec
[M::mem_process_seqs] Processed 675028 reads in 218.503 CPU sec, 7.119 real sec
sorting block 172, #reads = 1080683 mem = 536881983 peak = 1109897216
[M::process] read 674936 sequences (100000003 bp) in 2.391 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285039, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.50, 134.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 173, #reads = 1080214 mem = 536879015 peak = 1109897216
[M::process] read 674912 sequences (100000239 bp) in 2.155 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285189, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.95, 135.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674868 reads in 195.913 CPU sec, 6.284 real sec
[M::process] read 674812 sequences (100000133 bp) in 1.655 CPU sec, 1.692 real sec...
[M::mem_process_seqs] Processed 674936 reads in 120.720 CPU sec, 3.863 real sec
sorting block 174, #reads = 1081126 mem = 536880480 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284943, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.82, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674976 sequences (100000145 bp) in 2.664 CPU sec, 2.962 real sec...
[M::process] read 674834 sequences (100000245 bp) in 1.220 CPU sec, 1.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285298, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (259.76, 136.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 13, 2456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7362)
[M::mem_pestat] mean and std.dev: (753.90, 1117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9815)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674912 reads in 189.645 CPU sec, 6.089 real sec
[M::mem_process_seqs] Processed 674812 reads in 116.495 CPU sec, 3.767 real sec
sorting block 175, #reads = 1079977 mem = 536878011 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285291, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (260.36, 137.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 894)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674906 sequences (100000155 bp) in 2.628 CPU sec, 2.887 real sec...
[M::process] read 675272 sequences (100000296 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285275, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (259.91, 136.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674976 reads in 191.868 CPU sec, 6.147 real sec
[M::mem_process_seqs] Processed 674834 reads in 117.868 CPU sec, 3.804 real sec
sorting block 176, #reads = 1080047 mem = 536882163 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285100, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (260.22, 137.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676150 sequences (100000285 bp) in 2.100 CPU sec, 2.386 real sec...
[M::process] read 676046 sequences (100000024 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286202, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (253.52, 131.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674906 reads in 188.156 CPU sec, 6.036 real sec
sorting block 177, #reads = 1080791 mem = 536877275 peak = 1109897216
[M::mem_process_seqs] Processed 675272 reads in 113.136 CPU sec, 3.665 real sec
sorting block 178, #reads = 1079432 mem = 536880702 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286125, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 182, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (237.39, 115.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 2327, 2327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6931)
[M::mem_pestat] mean and std.dev: (1800.55, 1869.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675958 sequences (100000111 bp) in 2.319 CPU sec, 2.510 real sec...
[M::process] read 675956 sequences (100000087 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286293, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (239.50, 116.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676150 reads in 187.171 CPU sec, 6.011 real sec
[M::mem_process_seqs] Processed 676046 reads in 115.344 CPU sec, 3.731 real sec
sorting block 179, #reads = 1080869 mem = 536879757 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286251, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (240.45, 117.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675836 sequences (100000246 bp) in 2.155 CPU sec, 2.407 real sec...
[M::process] read 675904 sequences (100000190 bp) in 1.180 CPU sec, 1.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285846, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (241.70, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 126, 858)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552)
[M::mem_pestat] mean and std.dev: (347.77, 393.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3399)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675958 reads in 186.067 CPU sec, 5.985 real sec
[M::mem_process_seqs] Processed 675956 reads in 115.618 CPU sec, 3.748 real sec
sorting block 180, #reads = 1081409 mem = 536879687 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285876, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (241.82, 118.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675886 sequences (100000232 bp) in 2.160 CPU sec, 2.376 real sec...
[M::process] read 675728 sequences (100000036 bp) in 1.150 CPU sec, 1.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286288, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.26, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 13, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199)
[M::mem_pestat] mean and std.dev: (15.20, 18.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 264)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675836 reads in 187.320 CPU sec, 6.023 real sec
[M::mem_process_seqs] Processed 675904 reads in 115.463 CPU sec, 3.741 real sec
sorting block 181, #reads = 1080687 mem = 536879543 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286362, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.84, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 69, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (90.00, 82.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675798 sequences (100000283 bp) in 2.345 CPU sec, 2.720 real sec...
[M::process] read 675578 sequences (100000275 bp) in 1.080 CPU sec, 1.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286022, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.96, 121.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675886 reads in 187.553 CPU sec, 6.019 real sec
sorting block 182, #reads = 1081451 mem = 536879746 peak = 1109897216
[M::mem_process_seqs] Processed 675728 reads in 115.691 CPU sec, 3.747 real sec
sorting block 183, #reads = 1080865 mem = 536879492 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285748, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (243.83, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675672 sequences (100000067 bp) in 2.292 CPU sec, 2.664 real sec...
[M::process] read 675604 sequences (100000084 bp) in 1.140 CPU sec, 1.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285747, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (244.90, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (422, 447, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (328, 563)
[M::mem_pestat] mean and std.dev: (455.00, 37.82)
[M::mem_pestat] low and high boundaries for proper pairs: (281, 610)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675798 reads in 191.935 CPU sec, 6.172 real sec
[M::mem_process_seqs] Processed 675578 reads in 116.586 CPU sec, 3.776 real sec
sorting block 184, #reads = 1081398 mem = 536879421 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286036, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (245.51, 122.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675522 sequences (100000024 bp) in 2.577 CPU sec, 2.900 real sec...
[M::process] read 675474 sequences (100000251 bp) in 1.330 CPU sec, 1.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285860, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.60, 123.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (379, 415, 1414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3484)
[M::mem_pestat] mean and std.dev: (722.08, 586.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4519)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675672 reads in 190.479 CPU sec, 6.110 real sec
[M::mem_process_seqs] Processed 675604 reads in 116.324 CPU sec, 3.754 real sec
sorting block 185, #reads = 1080346 mem = 536879925 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285687, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (246.83, 123.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 18, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248)
[M::mem_pestat] mean and std.dev: (167.31, 208.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1663)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675524 sequences (100000288 bp) in 2.294 CPU sec, 2.643 real sec...
[M::process] read 675518 sequences (100000131 bp) in 1.120 CPU sec, 1.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285788, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.92, 124.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 22, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590)
[M::mem_pestat] mean and std.dev: (174.89, 230.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2114)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675522 reads in 189.884 CPU sec, 6.096 real sec
[M::mem_process_seqs] Processed 675474 reads in 116.164 CPU sec, 3.769 real sec
sorting block 186, #reads = 1080646 mem = 536881392 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285872, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (247.09, 124.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675488 sequences (100000174 bp) in 2.389 CPU sec, 2.775 real sec...
[M::process] read 675416 sequences (100000243 bp) in 1.140 CPU sec, 1.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285132, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (248.03, 125.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675524 reads in 191.579 CPU sec, 6.148 real sec
sorting block 187, #reads = 1081010 mem = 536878096 peak = 1109897216
[M::mem_process_seqs] Processed 675518 reads in 118.124 CPU sec, 3.826 real sec
sorting block 188, #reads = 1080290 mem = 536879977 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285340, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (248.77, 125.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 113, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800)
[M::mem_pestat] mean and std.dev: (255.80, 279.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2395)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675420 sequences (100000238 bp) in 2.261 CPU sec, 2.527 real sec...
[M::process] read 675314 sequences (100000213 bp) in 1.090 CPU sec, 1.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285714, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.18, 127.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 169, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876)
[M::mem_pestat] mean and std.dev: (345.25, 338.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2445)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675488 reads in 190.890 CPU sec, 6.133 real sec
[M::mem_process_seqs] Processed 675416 reads in 116.018 CPU sec, 3.769 real sec
sorting block 189, #reads = 1080545 mem = 536878729 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285135, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.66, 127.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 193, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (159.29, 89.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675554 sequences (100000065 bp) in 2.228 CPU sec, 2.606 real sec...
[M::process] read 675404 sequences (100000097 bp) in 1.090 CPU sec, 1.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285619, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.60, 128.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 42, 1503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4445)
[M::mem_pestat] mean and std.dev: (519.17, 859.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5916)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675420 reads in 191.808 CPU sec, 6.163 real sec
[M::mem_process_seqs] Processed 675314 reads in 117.225 CPU sec, 3.798 real sec
sorting block 190, #reads = 1080557 mem = 536881223 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285457, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.54, 124.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675372 sequences (100000267 bp) in 2.362 CPU sec, 2.742 real sec...
[M::process] read 675326 sequences (100000031 bp) in 1.080 CPU sec, 1.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285434, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.23, 125.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675554 reads in 193.370 CPU sec, 6.210 real sec
[M::mem_process_seqs] Processed 675404 reads in 118.577 CPU sec, 3.846 real sec
sorting block 191, #reads = 1080716 mem = 536881045 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285537, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.68, 124.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 192, #reads = 1081129 mem = 536880003 peak = 1109897216
[M::process] read 675286 sequences (100000111 bp) in 2.359 CPU sec, 2.752 real sec...
[M::process] read 675400 sequences (100000229 bp) in 1.100 CPU sec, 1.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285378, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (250.27, 125.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675372 reads in 188.846 CPU sec, 6.070 real sec
[M::mem_process_seqs] Processed 675326 reads in 118.782 CPU sec, 3.847 real sec
sorting block 193, #reads = 1080483 mem = 536877869 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285666, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.68, 128.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675338 sequences (100000183 bp) in 2.265 CPU sec, 2.606 real sec...
[M::process] read 675154 sequences (100000195 bp) in 1.090 CPU sec, 1.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285505, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.22, 129.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675286 reads in 196.019 CPU sec, 6.296 real sec
[M::mem_process_seqs] Processed 675400 reads in 119.439 CPU sec, 3.877 real sec
sorting block 194, #reads = 1080191 mem = 536876575 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285942, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.45, 129.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675208 sequences (100000090 bp) in 2.293 CPU sec, 2.604 real sec...
[M::process] read 675158 sequences (100000052 bp) in 1.100 CPU sec, 1.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284967, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.62, 130.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675338 reads in 193.119 CPU sec, 6.201 real sec
[M::mem_process_seqs] Processed 675154 reads in 117.718 CPU sec, 3.819 real sec
sorting block 195, #reads = 1080984 mem = 536878395 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285111, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.16, 130.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675070 sequences (100000137 bp) in 2.516 CPU sec, 2.925 real sec...
[M::process] read 675074 sequences (100000194 bp) in 1.110 CPU sec, 1.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285660, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.17, 131.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675208 reads in 193.899 CPU sec, 6.224 real sec
sorting block 196, #reads = 1080495 mem = 536878504 peak = 1109897216
[M::mem_process_seqs] Processed 675158 reads in 117.958 CPU sec, 3.821 real sec
sorting block 197, #reads = 1080698 mem = 536878840 peak = 1109897216
[M::process] read 674898 sequences (100000238 bp) in 1.903 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285403, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.42, 132.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675002 sequences (100000155 bp) in 1.130 CPU sec, 1.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284521, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.02, 131.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284882, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.37, 131.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675070 reads in 280.116 CPU sec, 9.015 real sec
[M::mem_process_seqs] Processed 675074 reads in 204.499 CPU sec, 6.641 real sec
[M::mem_process_seqs] Processed 674898 reads in 218.776 CPU sec, 7.153 real sec
sorting block 198, #reads = 1080922 mem = 536879176 peak = 1109897216
[M::process] read 675030 sequences (100000126 bp) in 2.606 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285080, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (256.33, 132.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674970 sequences (100000233 bp) in 2.273 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285636, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.21, 133.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675002 reads in 202.076 CPU sec, 6.506 real sec
sorting block 199, #reads = 1079929 mem = 536883894 peak = 1109897216
[M::mem_process_seqs] Processed 675030 reads in 124.301 CPU sec, 3.988 real sec
[M::process] read 674948 sequences (100000047 bp) in 1.640 CPU sec, 1.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285006, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (258.16, 134.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 217, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (185.62, 104.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 602)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 200, #reads = 1080759 mem = 536879375 peak = 1109897216
[M::process] read 675008 sequences (100000130 bp) in 2.662 CPU sec, 3.132 real sec...
[M::process] read 674898 sequences (100000156 bp) in 1.040 CPU sec, 1.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284653, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.41, 133.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674970 reads in 199.996 CPU sec, 6.437 real sec
[M::mem_process_seqs] Processed 674948 reads in 122.539 CPU sec, 3.968 real sec
sorting block 201, #reads = 1080294 mem = 536876828 peak = 1109897216
[M::process] read 674798 sequences (100000188 bp) in 1.980 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284989, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (258.82, 134.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674776 sequences (100000226 bp) in 1.130 CPU sec, 1.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285781, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (259.26, 134.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285131, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (260.50, 136.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675008 reads in 276.296 CPU sec, 8.888 real sec
[M::mem_process_seqs] Processed 674898 reads in 199.645 CPU sec, 6.482 real sec
[M::mem_process_seqs] Processed 674798 reads in 214.364 CPU sec, 7.010 real sec
sorting block 202, #reads = 1080200 mem = 536879474 peak = 1109897216
[M::process] read 674830 sequences (100000216 bp) in 1.991 CPU sec, 2.215 real sec...
sorting block 203, #reads = 1080481 mem = 536878916 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285056, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.59, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674904 sequences (100000013 bp) in 1.709 CPU sec, 1.860 real sec...
[M::process] read 674786 sequences (100000135 bp) in 1.140 CPU sec, 1.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285142, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.66, 138.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674776 reads in 196.448 CPU sec, 6.318 real sec
[M::mem_process_seqs] Processed 674830 reads in 119.457 CPU sec, 3.859 real sec
sorting block 204, #reads = 1080334 mem = 536877190 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285427, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (261.30, 137.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 898)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 26, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (98.00, 115.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674748 sequences (100000005 bp) in 2.211 CPU sec, 2.465 real sec...
[M::process] read 674954 sequences (100000223 bp) in 1.130 CPU sec, 1.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285379, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (263.27, 139.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 61, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (101.18, 112.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674904 reads in 190.143 CPU sec, 6.110 real sec
[M::mem_process_seqs] Processed 674786 reads in 116.390 CPU sec, 3.786 real sec
sorting block 205, #reads = 1079927 mem = 536878326 peak = 1109897216
[M::process] read 676144 sequences (100000230 bp) in 1.792 CPU sec, 1.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285407, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (263.26, 139.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676038 sequences (100000232 bp) in 1.120 CPU sec, 1.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284982, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718)
[M::mem_pestat] mean and std.dev: (260.93, 138.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286518, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 182, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (236.18, 114.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674748 reads in 268.948 CPU sec, 8.657 real sec
sorting block 206, #reads = 1080127 mem = 536880101 peak = 1109897216
[M::mem_process_seqs] Processed 674954 reads in 196.269 CPU sec, 6.370 real sec
[M::mem_process_seqs] Processed 676144 reads in 210.312 CPU sec, 6.874 real sec
sorting block 207, #reads = 1079452 mem = 536878599 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286259, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.09, 116.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 39, 39)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 103)
[M::mem_pestat] mean and std.dev: (24.22, 17.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 135)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676030 sequences (100000225 bp) in 2.562 CPU sec, 2.976 real sec...
[M::mem_process_seqs] Processed 676038 reads in 104.926 CPU sec, 3.492 real sec
[M::process] read 675924 sequences (100000076 bp) in 1.928 CPU sec, 2.150 real sec...
sorting block 208, #reads = 1080305 mem = 536879182 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286459, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (239.39, 116.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675924 sequences (100000147 bp) in 1.575 CPU sec, 1.673 real sec...
[M::process] read 675950 sequences (100000159 bp) in 1.110 CPU sec, 1.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286702, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (239.77, 116.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676030 reads in 185.112 CPU sec, 5.938 real sec
[M::mem_process_seqs] Processed 675924 reads in 114.305 CPU sec, 3.702 real sec
sorting block 209, #reads = 1081347 mem = 536882278 peak = 1109897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286033, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (240.51, 117.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675802 sequences (100000274 bp) in 2.124 CPU sec, 2.376 real sec...
[M::process] read 675736 sequences (100000215 bp) in 1.110 CPU sec, 1.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286050, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 183, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (241.37, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675924 reads in 185.105 CPU sec, 5.954 real sec
[M::mem_process_seqs] Processed 675950 reads in 114.194 CPU sec, 3.703 real sec
sorting block 210, #reads = 1080931 mem = 536879018 peak = 1109897216
[M::process] read 675696 sequences (100000258 bp) in 1.720 CPU sec, 1.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285949, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (241.93, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675894 sequences (100000000 bp) in 1.110 CPU sec, 1.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286104, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.27, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285698, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.26, 120.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675802 reads in 268.046 CPU sec, 8.627 real sec
sorting block 211, #reads = 1080593 mem = 536878328 peak = 1109897216
[M::mem_process_seqs] Processed 675736 reads in 194.480 CPU sec, 6.304 real sec
[M::mem_process_seqs] Processed 675696 reads in 208.762 CPU sec, 6.816 real sec
sorting block 212, #reads = 1081133 mem = 536878722 peak = 1109897216
[M::process] read 675512 sequences (100000112 bp) in 2.034 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285510, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 184, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (243.48, 120.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675418 sequences (100000283 bp) in 1.542 CPU sec, 1.651 real sec...
[M::process] read 674732 sequences (100000084 bp) in 1.120 CPU sec, 1.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285896, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (245.46, 122.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675894 reads in 190.167 CPU sec, 6.108 real sec
sorting block 213, #reads = 1080364 mem = 536878743 peak = 1109897216
[M::mem_process_seqs] Processed 675512 reads in 117.527 CPU sec, 3.792 real sec
sorting block 214, #reads = 1081203 mem = 536878745 peak = 1109897216
[M::process] read 675362 sequences (100000134 bp) in 1.984 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285322, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.23, 125.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675648 sequences (100000141 bp) in 1.130 CPU sec, 1.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284468, 14, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (269.50, 143.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 9, 14)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 32)
[M::mem_pestat] mean and std.dev: (7.18, 3.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 41)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285280, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.40, 125.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 380, 1741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5197)
[M::mem_pestat] mean and std.dev: (733.53, 748.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6925)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675418 reads in 276.667 CPU sec, 8.902 real sec
[M::mem_process_seqs] Processed 674732 reads in 202.282 CPU sec, 6.554 real sec
[M::mem_process_seqs] Processed 675362 reads in 216.295 CPU sec, 7.056 real sec
sorting block 215, #reads = 1080577 mem = 536880130 peak = 1109897216
[M::process] read 675448 sequences (100000277 bp) in 2.067 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285397, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 185, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (247.00, 124.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 145, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (130.89, 125.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675394 sequences (100000266 bp) in 2.372 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285549, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 185, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (248.57, 125.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 32, 99)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245)
[M::mem_pestat] mean and std.dev: (54.64, 41.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 318)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675456 sequences (100000245 bp) in 1.110 CPU sec, 1.107 real sec...
[M::mem_process_seqs] Processed 675648 reads in 195.883 CPU sec, 6.284 real sec
sorting block 216, #reads = 1080315 mem = 536878950 peak = 1109897216
[M::mem_process_seqs] Processed 675448 reads in 119.817 CPU sec, 3.853 real sec
sorting block 217, #reads = 1080574 mem = 536879285 peak = 1111035904
[M::process] read 675312 sequences (100000189 bp) in 1.792 CPU sec, 1.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285566, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.77, 126.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675216 sequences (100000098 bp) in 1.080 CPU sec, 1.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285609, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.35, 127.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 58, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278)
[M::mem_pestat] mean and std.dev: (54.10, 37.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 365)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284842, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.96, 127.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675394 reads in 278.363 CPU sec, 8.962 real sec
[M::mem_process_seqs] Processed 675456 reads in 203.258 CPU sec, 6.599 real sec
[M::mem_process_seqs] Processed 675312 reads in 218.667 CPU sec, 7.151 real sec
sorting block 218, #reads = 1080546 mem = 536880144 peak = 1111035904
[M::process] read 675250 sequences (100000025 bp) in 2.317 CPU sec, 2.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285245, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (251.46, 128.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 219, #reads = 1080421 mem = 536880977 peak = 1111035904
[M::process] read 675196 sequences (100000181 bp) in 1.621 CPU sec, 1.737 real sec...
[M::process] read 675172 sequences (100000145 bp) in 1.120 CPU sec, 1.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285032, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.90, 126.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675216 reads in 197.597 CPU sec, 6.360 real sec
[M::mem_process_seqs] Processed 675250 reads in 120.933 CPU sec, 3.898 real sec
sorting block 220, #reads = 1080556 mem = 536883860 peak = 1111035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285760, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.84, 126.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 202, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (144.93, 84.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674650 sequences (100000209 bp) in 2.550 CPU sec, 3.005 real sec...
[M::process] read 675102 sequences (100000191 bp) in 1.070 CPU sec, 1.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285454, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.29, 126.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 268, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429)
[M::mem_pestat] mean and std.dev: (270.55, 226.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1885)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675196 reads in 192.052 CPU sec, 6.170 real sec
[M::mem_process_seqs] Processed 675172 reads in 117.572 CPU sec, 3.808 real sec
sorting block 221, #reads = 1080978 mem = 536879451 peak = 1111035904
[M::process] read 675110 sequences (100000297 bp) in 1.831 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285127, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.18, 134.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 14, 4300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12894)
[M::mem_pestat] mean and std.dev: (1203.18, 1899.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17191)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675162 sequences (100000083 bp) in 1.100 CPU sec, 1.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285062, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (252.83, 128.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 31, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (128.00, 243.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285845, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.48, 129.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674650 reads in 277.196 CPU sec, 8.924 real sec
[M::mem_process_seqs] Processed 675102 reads in 201.142 CPU sec, 6.522 real sec
sorting block 222, #reads = 1080436 mem = 536878981 peak = 1111035904
[M::mem_process_seqs] Processed 675110 reads in 216.044 CPU sec, 7.064 real sec
sorting block 223, #reads = 1080384 mem = 536880471 peak = 1111035904
[M::process] read 675252 sequences (100000078 bp) in 2.472 CPU sec, 2.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285120, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.23, 129.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675268 sequences (100000096 bp) in 2.165 CPU sec, 2.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285134, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.21, 129.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675162 reads in 196.159 CPU sec, 6.311 real sec
[M::mem_process_seqs] Processed 675252 reads in 122.860 CPU sec, 3.943 real sec
[M::process] read 675088 sequences (100000199 bp) in 1.352 CPU sec, 1.407 real sec...
sorting block 224, #reads = 1080030 mem = 536878618 peak = 1111035904
[M::process] read 675060 sequences (100000093 bp) in 1.891 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284831, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (253.29, 130.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674550 sequences (100000150 bp) in 1.050 CPU sec, 1.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285672, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.11, 133.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285146, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (254.66, 130.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675268 reads in 280.401 CPU sec, 9.035 real sec
sorting block 225, #reads = 1080175 mem = 536876891 peak = 1111035904
[M::mem_process_seqs] Processed 675060 reads in 203.604 CPU sec, 6.604 real sec
[M::mem_process_seqs] Processed 675088 reads in 218.348 CPU sec, 7.133 real sec
sorting block 226, #reads = 1080783 mem = 536879434 peak = 1111035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284277, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (267.33, 142.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (58, 74, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (114.69, 82.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675100 sequences (100000224 bp) in 2.758 CPU sec, 3.278 real sec...
[M::mem_process_seqs] Processed 674550 reads in 111.048 CPU sec, 3.695 real sec
[M::process] read 674966 sequences (100000010 bp) in 2.488 CPU sec, 2.972 real sec...
sorting block 227, #reads = 1079945 mem = 536879716 peak = 1111035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285245, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (255.93, 132.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674886 sequences (100000284 bp) in 1.715 CPU sec, 1.893 real sec...
[M::process] read 674890 sequences (100000095 bp) in 1.120 CPU sec, 1.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285212, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.36, 133.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675100 reads in 200.292 CPU sec, 6.430 real sec
[M::mem_process_seqs] Processed 674966 reads in 123.119 CPU sec, 3.969 real sec
sorting block 228, #reads = 1080480 mem = 536878380 peak = 1111035904
[M::process] read 674886 sequences (100000006 bp) in 1.929 CPU sec, 2.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285048, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (257.20, 132.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674750 sequences (100000067 bp) in 1.110 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285165, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709)
[M::mem_pestat] mean and std.dev: (258.89, 134.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 28, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (81.33, 88.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284438, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (258.51, 133.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674886 reads in 281.513 CPU sec, 9.065 real sec
[M::mem_process_seqs] Processed 674890 reads in 205.033 CPU sec, 6.642 real sec
sorting block 229, #reads = 1080691 mem = 536879389 peak = 1111035904
[M::mem_process_seqs] Processed 674886 reads in 219.826 CPU sec, 7.173 real sec
[M::process] read 674530 sequences (100000007 bp) in 1.783 CPU sec, 2.011 real sec...
sorting block 230, #reads = 1080389 mem = 536881407 peak = 1111035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285158, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (260.71, 135.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 85, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202)
[M::mem_pestat] mean and std.dev: (197.55, 223.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1599)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674886 sequences (100000242 bp) in 2.569 CPU sec, 2.991 real sec...
[M::process] read 674502 sequences (100000068 bp) in 1.040 CPU sec, 1.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284859, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (271.92, 146.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674750 reads in 205.732 CPU sec, 6.603 real sec
[M::mem_process_seqs] Processed 674530 reads in 123.738 CPU sec, 3.987 real sec
sorting block 231, #reads = 1079052 mem = 536879223 peak = 1111035904
[M::process] read 674642 sequences (100000269 bp) in 1.868 CPU sec, 2.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285096, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (261.86, 137.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 5, 61)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177)
[M::mem_pestat] mean and std.dev: (24.73, 31.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 235)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674782 sequences (100000044 bp) in 1.070 CPU sec, 1.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285205, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 193, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (270.44, 144.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 183, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082)
[M::mem_pestat] mean and std.dev: (174.73, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1439)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285030, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (265.26, 140.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 247, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (173.78, 206.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1441)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674886 reads in 277.600 CPU sec, 8.942 real sec
[M::mem_process_seqs] Processed 674502 reads in 201.966 CPU sec, 6.548 real sec
sorting block 232, #reads = 1078510 mem = 536878669 peak = 1111035904
[M::mem_process_seqs] Processed 674642 reads in 216.980 CPU sec, 7.085 real sec
sorting block 233, #reads = 1080901 mem = 536877302 peak = 1111035904
[M::process] read 674708 sequences (100000081 bp) in 2.101 CPU sec, 2.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285451, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (262.19, 137.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674692 sequences (100000140 bp) in 2.216 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284906, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (263.86, 139.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674682 sequences (100000231 bp) in 1.090 CPU sec, 1.093 real sec...
[M::mem_process_seqs] Processed 674782 reads in 199.273 CPU sec, 6.389 real sec
[M::mem_process_seqs] Processed 674708 reads in 121.539 CPU sec, 3.917 real sec
sorting block 234, #reads = 1080425 mem = 536878147 peak = 1111035904
[M::process] read 361406 sequences (53572210 bp) in 1.090 CPU sec, 1.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284582, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (263.72, 139.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284861, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (263.65, 139.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 72, 806)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374)
[M::mem_pestat] mean and std.dev: (241.33, 319.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3158)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 152378, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.20, 141.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674692 reads in 235.960 CPU sec, 7.648 real sec
sorting block 235, #reads = 1080298 mem = 536878573 peak = 1111035904
[M::mem_process_seqs] Processed 674682 reads in 160.522 CPU sec, 5.241 real sec
[M::mem_process_seqs] Processed 361406 reads in 168.261 CPU sec, 5.517 real sec
sorting block 236, #reads = 1079697 mem = 536879502 peak = 1111035904
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 254240560
overall: 69892055040 mem 36670.814 user 3855.173 sys 1342.116 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003322.L006\tPU:L006\tSM:T21-D0003322\tLB:T21-D0003322\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 T21-D0003322_R1.fastq.gz T21-D0003322_R2.fastq.gz
[main] Real time: 1342.146 sec; CPU: 40525.990 sec
sorting block 237, #reads = 564368 mem = 280857429 peak = 1111035904
uniq header count 1
merging 238 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 8615785727
output reads: 256653165
bam_mem_sort: 238 calls 86.057 user 0.681 sys 87.169 real
bam_write: 238 calls 90.749 user 2.875 sys 95.690 real
execute: 1 calls 65.922 user 5.293 sys 1416.681 real
merge_files: 1 calls 65.904 user 5.268 sys 73.943 real
parse_chunk: 3801 calls 111.815 user 35.617 sys 151.928 real
read_chunk: 10229 calls 133.301 user 105.157 sys 1289.199 real
sort_block: 1 calls 104.340 user 104.121 sys 1247.212 real
write_chunk: 6426 calls 65.444 user 4.841 sys 71.304 real
overall: 2748473344 mem 2148.829 user 180.417 sys 1416.700 real
ls: cannot access '*.cram': No such file or directory