Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a9/f8761b7f124e087aed173e56d85f31/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/61/a927c426fef022751b0c0fb5dca416/T21-D0003376/0004.T21-D0003376_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/61/a927c426fef022751b0c0fb5dca416/T21-D0003376/0004.T21-D0003376_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a9/f8761b7f124e087aed173e56d85f31/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
Received only 28 licenses out of 31 requested for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
Received only 28 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 2652.195 CPU sec, 113.329 real sec
[M::process] read 677622 sequences (100000280 bp) in 1.126 CPU sec, 1.129 real sec...
[M::process] read 677482 sequences (100000121 bp) in 1.380 CPU sec, 1.382 real sec...
[M::process] read 677308 sequences (100000164 bp) in 1.339 CPU sec, 1.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288945, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (214.67, 92.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (523, 549, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 1234)
[M::mem_pestat] mean and std.dev: (542.00, 204.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1471)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677112 sequences (100000113 bp) in 1.120 CPU sec, 1.111 real sec...
Received only 28 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288530, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (221.29, 100.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288837, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.36, 102.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677622 reads in 294.661 CPU sec, 10.538 real sec
[M::mem_process_seqs] Processed 677482 reads in 203.447 CPU sec, 7.425 real sec
[M::mem_process_seqs] Processed 677308 reads in 212.357 CPU sec, 7.787 real sec
sorting block 0, #reads = 1082682 mem = 536879847 peak = 605413376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288457, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (224.18, 104.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677048 sequences (100000094 bp) in 3.645 CPU sec, 4.203 real sec...
[M::mem_process_seqs] Processed 677112 reads in 116.739 CPU sec, 4.247 real sec
sorting block 1, #reads = 1082868 mem = 536878932 peak = 728096768
[M::process] read 676880 sequences (100000061 bp) in 2.829 CPU sec, 3.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287819, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.72, 105.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 65, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217)
[M::mem_pestat] mean and std.dev: (56.56, 36.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 280)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676842 sequences (100000254 bp) in 1.530 CPU sec, 1.524 real sec...
[M::process] read 676726 sequences (100000077 bp) in 1.764 CPU sec, 1.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288176, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.14, 108.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677048 reads in 223.260 CPU sec, 7.846 real sec
[M::mem_process_seqs] Processed 676880 reads in 124.618 CPU sec, 4.407 real sec
sorting block 2, #reads = 1082557 mem = 536877475 peak = 728096768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288048, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.51, 110.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676534 sequences (100000179 bp) in 3.583 CPU sec, 4.103 real sec...
[M::process] read 676554 sequences (100000296 bp) in 1.770 CPU sec, 1.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287663, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.17, 111.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676842 reads in 234.024 CPU sec, 8.319 real sec
[M::mem_process_seqs] Processed 676726 reads in 130.868 CPU sec, 4.650 real sec
sorting block 3, #reads = 1082579 mem = 536880530 peak = 728096768
sorting block 4, #reads = 1082034 mem = 536878967 peak = 844328960
[M::process] read 676436 sequences (100000240 bp) in 2.731 CPU sec, 3.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288202, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.19, 113.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 219, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130)
[M::mem_pestat] mean and std.dev: (268.70, 241.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1471)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676072 sequences (100000017 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288026, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.37, 115.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287280, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.47, 114.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676534 reads in 334.619 CPU sec, 11.988 real sec
[M::mem_process_seqs] Processed 676436 reads in 234.527 CPU sec, 8.482 real sec
[M::mem_process_seqs] Processed 676554 reads in 244.780 CPU sec, 8.915 real sec
sorting block 5, #reads = 1082355 mem = 536877827 peak = 844328960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287404, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.43, 118.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676570 sequences (100000075 bp) in 3.896 CPU sec, 4.526 real sec...
[M::mem_process_seqs] Processed 676072 reads in 129.035 CPU sec, 4.756 real sec
sorting block 6, #reads = 1081566 mem = 536878108 peak = 844328960
[M::process] read 677456 sequences (100000228 bp) in 3.586 CPU sec, 4.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288194, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.03, 114.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677506 sequences (100000161 bp) in 1.780 CPU sec, 1.779 real sec...
[M::process] read 677194 sequences (100000184 bp) in 1.680 CPU sec, 1.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288417, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.86, 97.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676570 reads in 233.603 CPU sec, 8.327 real sec
[M::mem_process_seqs] Processed 677456 reads in 123.692 CPU sec, 4.475 real sec
sorting block 7, #reads = 1081544 mem = 536878308 peak = 844328960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288675, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.30, 102.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 23, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (53.70, 80.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677182 sequences (100000037 bp) in 3.767 CPU sec, 4.175 real sec...
Received only 28 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288487, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.45, 105.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676778 sequences (100000143 bp) in 2.180 CPU sec, 2.182 real sec...
[M::mem_process_seqs] Processed 677506 reads in 218.480 CPU sec, 7.793 real sec
[M::mem_process_seqs] Processed 677194 reads in 120.646 CPU sec, 4.268 real sec
sorting block 8, #reads = 1082916 mem = 536879955 peak = 844328960
sorting block 9, #reads = 1082631 mem = 536879154 peak = 844328960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288605, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.29, 106.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 51, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (187.70, 233.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1411)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676868 sequences (100000000 bp) in 2.860 CPU sec, 3.090 real sec...
[M::process] read 676878 sequences (100000089 bp) in 1.830 CPU sec, 1.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288545, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.29, 108.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677182 reads in 206.011 CPU sec, 7.278 real sec
[M::mem_process_seqs] Processed 676778 reads in 113.197 CPU sec, 4.007 real sec
sorting block 10, #reads = 1082656 mem = 536880596 peak = 844328960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288517, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676622 sequences (100000017 bp) in 2.513 CPU sec, 2.815 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.79, 110.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676544 sequences (100000054 bp) in 1.660 CPU sec, 1.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288014, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.63, 110.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 125, 3968)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11830)
[M::mem_pestat] mean and std.dev: (2410.80, 3760.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17452)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676868 reads in 205.863 CPU sec, 7.289 real sec
[M::mem_process_seqs] Processed 676878 reads in 114.079 CPU sec, 4.051 real sec
sorting block 11, #reads = 1082330 mem = 536878355 peak = 844328960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287625, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.43, 111.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 87, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (93.31, 107.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676398 sequences (100000253 bp) in 3.060 CPU sec, 3.394 real sec...
[M::process] read 676466 sequences (100000152 bp) in 2.131 CPU sec, 2.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287636, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.15, 113.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676622 reads in 210.656 CPU sec, 7.419 real sec
[M::mem_process_seqs] Processed 676544 reads in 117.330 CPU sec, 4.153 real sec
sorting block 12, #reads = 1082415 mem = 536878723 peak = 844328960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288247, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.97, 115.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676256 sequences (100000239 bp) in 3.535 CPU sec, 3.865 real sec...
[M::process] read 677116 sequences (100000086 bp) in 2.240 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288277, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.48, 115.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676398 reads in 207.689 CPU sec, 7.317 real sec
sorting block 13, #reads = 1082250 mem = 536878785 peak = 844328960
[M::mem_process_seqs] Processed 676466 reads in 115.010 CPU sec, 4.036 real sec
sorting block 14, #reads = 1082181 mem = 536879686 peak = 872468480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288306, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.44, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677464 sequences (100000048 bp) in 3.852 CPU sec, 4.145 real sec...
[M::process] read 677204 sequences (100000200 bp) in 1.830 CPU sec, 1.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288973, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.97, 107.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676256 reads in 205.174 CPU sec, 7.221 real sec
[M::mem_process_seqs] Processed 677116 reads in 115.246 CPU sec, 4.047 real sec
sorting block 15, #reads = 1082450 mem = 536877934 peak = 872468480
[M::process] read 677166 sequences (100000298 bp) in 3.518 CPU sec, 3.930 real sec...
[M::process] read 677182 sequences (100000078 bp) in 1.880 CPU sec, 1.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288592, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.40, 105.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677204 reads in 104.258 CPU sec, 3.637 real sec
sorting block 16, #reads = 1082751 mem = 536879157 peak = 872468480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288593, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (220.08, 99.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676902 sequences (100000222 bp) in 1.603 CPU sec, 1.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288512, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.01, 105.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677464 reads in 302.516 CPU sec, 10.675 real sec
[M::mem_process_seqs] Processed 677166 reads in 120.251 CPU sec, 4.290 real sec
sorting block 17, #reads = 1082531 mem = 536880101 peak = 872468480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288333, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.78, 106.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676838 sequences (100000169 bp) in 3.307 CPU sec, 3.627 real sec...
[M::process] read 676718 sequences (100000235 bp) in 1.819 CPU sec, 1.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288265, 13, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.59, 109.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 566, 4564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13340)
[M::mem_pestat] mean and std.dev: (1356.92, 1779.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17728)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677182 reads in 203.598 CPU sec, 7.172 real sec
[M::process] read 676544 sequences (100000103 bp) in 1.747 CPU sec, 1.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288376, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.94, 111.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676838 reads in 114.373 CPU sec, 3.816 real sec
sorting block 18, #reads = 1082746 mem = 536879157 peak = 872468480
[M::mem_process_seqs] Processed 676902 reads in 273.630 CPU sec, 9.304 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288616, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.61, 113.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1082116 mem = 536875776 peak = 952909824
[M::process] read 676500 sequences (100000013 bp) in 2.638 CPU sec, 2.916 real sec...
[M::process] read 676500 sequences (100000069 bp) in 1.864 CPU sec, 1.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288009, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.84, 113.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676718 reads in 229.436 CPU sec, 7.374 real sec
[M::mem_process_seqs] Processed 676544 reads in 123.142 CPU sec, 3.935 real sec
sorting block 20, #reads = 1082831 mem = 536880624 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287674, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.12, 115.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676218 sequences (100000272 bp) in 2.487 CPU sec, 2.690 real sec...
[M::process] read 676154 sequences (100000038 bp) in 1.647 CPU sec, 1.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288137, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.22, 115.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676500 reads in 213.748 CPU sec, 6.848 real sec
[M::mem_process_seqs] Processed 676500 reads in 117.329 CPU sec, 3.749 real sec
sorting block 21, #reads = 1082234 mem = 536877748 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287732, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.49, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677576 sequences (100000078 bp) in 3.218 CPU sec, 3.503 real sec...
[M::process] read 677504 sequences (100000145 bp) in 1.538 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288330, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.89, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 78, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797)
[M::mem_pestat] mean and std.dev: (263.27, 288.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2374)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676218 reads in 212.274 CPU sec, 6.789 real sec
[M::mem_process_seqs] Processed 676154 reads in 117.625 CPU sec, 3.746 real sec
sorting block 22, #reads = 1082503 mem = 536879129 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288961, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (218.12, 96.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 41, 646)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1904)
[M::mem_pestat] mean and std.dev: (291.00, 376.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2533)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677208 sequences (100000001 bp) in 2.538 CPU sec, 2.750 real sec...
[M::process] read 677066 sequences (100000160 bp) in 1.780 CPU sec, 1.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288703, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (221.14, 101.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677576 reads in 212.618 CPU sec, 6.813 real sec
sorting block 23, #reads = 1081948 mem = 536880690 peak = 952909824
[M::mem_process_seqs] Processed 677504 reads in 118.261 CPU sec, 3.770 real sec
sorting block 24, #reads = 1082819 mem = 536879678 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288296, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.19, 103.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 11, 12)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 30)
[M::mem_pestat] mean and std.dev: (6.25, 4.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 39)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677054 sequences (100000217 bp) in 2.898 CPU sec, 3.113 real sec...
[M::process] read 676866 sequences (100000289 bp) in 1.768 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288271, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.22, 105.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677208 reads in 215.633 CPU sec, 6.909 real sec
[M::mem_process_seqs] Processed 677066 reads in 119.394 CPU sec, 3.800 real sec
sorting block 25, #reads = 1082066 mem = 536881114 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288515, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.70, 106.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 47, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236)
[M::mem_pestat] mean and std.dev: (238.20, 305.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1641)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676588 sequences (100000045 bp) in 2.615 CPU sec, 2.860 real sec...
[M::process] read 676628 sequences (100000194 bp) in 1.833 CPU sec, 1.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288191, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.15, 109.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 448, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281)
[M::mem_pestat] mean and std.dev: (336.90, 264.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1682)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677054 reads in 215.406 CPU sec, 6.900 real sec
[M::mem_process_seqs] Processed 676866 reads in 119.339 CPU sec, 3.800 real sec
sorting block 26, #reads = 1082595 mem = 536878458 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288202, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.41, 110.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676552 sequences (100000022 bp) in 3.084 CPU sec, 3.342 real sec...
[M::process] read 676528 sequences (100000112 bp) in 1.882 CPU sec, 1.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287892, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.34, 113.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 75, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1812)
[M::mem_pestat] mean and std.dev: (274.83, 492.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2413)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676588 reads in 217.544 CPU sec, 6.957 real sec
[M::mem_process_seqs] Processed 676628 reads in 120.847 CPU sec, 3.846 real sec
sorting block 27, #reads = 1082347 mem = 536879960 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287641, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.87, 113.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676378 sequences (100000148 bp) in 2.588 CPU sec, 2.810 real sec...
[M::process] read 676190 sequences (100000100 bp) in 1.682 CPU sec, 1.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287699, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.21, 115.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 965, 965)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713)
[M::mem_pestat] mean and std.dev: (597.27, 437.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3587)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676552 reads in 215.522 CPU sec, 6.914 real sec
sorting block 28, #reads = 1082844 mem = 536879069 peak = 952909824
[M::mem_process_seqs] Processed 676528 reads in 118.136 CPU sec, 3.766 real sec
sorting block 29, #reads = 1082139 mem = 536879929 peak = 952909824
[M::process] read 676464 sequences (100000082 bp) in 2.918 CPU sec, 3.145 real sec...
[M::process] read 677722 sequences (100000271 bp) in 1.413 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287985, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.80, 118.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 79, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450)
[M::mem_pestat] mean and std.dev: (296.20, 427.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2005)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676190 reads in 110.724 CPU sec, 3.521 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287986, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.63, 117.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288306, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.78, 115.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677630 sequences (100000198 bp) in 1.839 CPU sec, 1.840 real sec...
[M::mem_process_seqs] Processed 676378 reads in 323.467 CPU sec, 10.371 real sec
[M::mem_process_seqs] Processed 676464 reads in 127.597 CPU sec, 4.112 real sec
sorting block 30, #reads = 1081886 mem = 536878250 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288562, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (211.01, 88.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1081800 mem = 536879239 peak = 952909824
[M::process] read 677484 sequences (100000195 bp) in 3.066 CPU sec, 3.342 real sec...
[M::process] read 677412 sequences (100000131 bp) in 1.937 CPU sec, 1.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288631, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (213.61, 92.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677722 reads in 219.766 CPU sec, 7.037 real sec
[M::mem_process_seqs] Processed 677630 reads in 123.753 CPU sec, 3.932 real sec
sorting block 32, #reads = 1083176 mem = 536877852 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287896, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (218.37, 98.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677188 sequences (100000148 bp) in 2.569 CPU sec, 2.808 real sec...
[M::process] read 677070 sequences (100000200 bp) in 1.912 CPU sec, 1.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288651, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.49, 99.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677484 reads in 220.192 CPU sec, 7.049 real sec
[M::mem_process_seqs] Processed 677412 reads in 123.859 CPU sec, 3.941 real sec
sorting block 33, #reads = 1082744 mem = 536877544 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288250, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.60, 104.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677010 sequences (100000198 bp) in 2.867 CPU sec, 3.150 real sec...
[M::process] read 676912 sequences (100000071 bp) in 1.969 CPU sec, 1.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287707, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.90, 107.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677188 reads in 225.855 CPU sec, 7.245 real sec
[M::mem_process_seqs] Processed 677070 reads in 126.351 CPU sec, 4.017 real sec
sorting block 34, #reads = 1083161 mem = 536878394 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287847, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.77, 109.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676852 sequences (100000194 bp) in 2.719 CPU sec, 2.962 real sec...
[M::process] read 676628 sequences (100000220 bp) in 1.868 CPU sec, 1.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287740, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.42, 110.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677010 reads in 226.152 CPU sec, 7.246 real sec
sorting block 35, #reads = 1082753 mem = 536878733 peak = 952909824
[M::mem_process_seqs] Processed 676912 reads in 127.226 CPU sec, 4.045 real sec
sorting block 36, #reads = 1082709 mem = 536880886 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287543, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.82, 111.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000154 bp) in 2.674 CPU sec, 2.923 real sec...
[M::process] read 676674 sequences (100000170 bp) in 1.628 CPU sec, 1.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287296, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.64, 112.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 34, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466)
[M::mem_pestat] mean and std.dev: (53.22, 50.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676852 reads in 224.812 CPU sec, 7.218 real sec
[M::mem_process_seqs] Processed 676628 reads in 126.506 CPU sec, 4.033 real sec
sorting block 37, #reads = 1082390 mem = 536880545 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287675, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.28, 113.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677708 sequences (100000262 bp) in 2.811 CPU sec, 3.064 real sec...
[M::process] read 677614 sequences (100000062 bp) in 2.012 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287278, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.90, 116.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 225.800 CPU sec, 7.234 real sec
[M::mem_process_seqs] Processed 676674 reads in 126.073 CPU sec, 4.009 real sec
sorting block 38, #reads = 1082293 mem = 536878869 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288842, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (210.61, 88.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 645)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677454 sequences (100000174 bp) in 2.986 CPU sec, 3.275 real sec...
[M::process] read 677472 sequences (100000202 bp) in 2.018 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288622, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (209.91, 87.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677708 reads in 221.831 CPU sec, 7.098 real sec
[M::mem_process_seqs] Processed 677614 reads in 123.871 CPU sec, 3.939 real sec
sorting block 39, #reads = 1082243 mem = 536878336 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288345, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.19, 100.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 58, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (161.55, 168.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677366 sequences (100000174 bp) in 2.810 CPU sec, 3.103 real sec...
[M::process] read 677252 sequences (100000201 bp) in 1.952 CPU sec, 1.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288315, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.51, 99.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677454 reads in 227.813 CPU sec, 7.304 real sec
sorting block 40, #reads = 1083059 mem = 536879704 peak = 952909824
[M::mem_process_seqs] Processed 677472 reads in 128.297 CPU sec, 4.086 real sec
sorting block 41, #reads = 1082185 mem = 536879107 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288140, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.49, 103.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677078 sequences (100000283 bp) in 2.928 CPU sec, 3.204 real sec...
[M::process] read 676966 sequences (100000285 bp) in 2.023 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288249, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.00, 107.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677366 reads in 227.133 CPU sec, 7.279 real sec
[M::mem_process_seqs] Processed 677252 reads in 127.314 CPU sec, 4.051 real sec
sorting block 42, #reads = 1082165 mem = 536879809 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288113, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.74, 108.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 560, 1406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4200)
[M::mem_pestat] mean and std.dev: (797.10, 948.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5597)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677012 sequences (100000246 bp) in 2.963 CPU sec, 3.228 real sec...
[M::process] read 676928 sequences (100000229 bp) in 1.959 CPU sec, 1.965 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287734, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.01, 110.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677078 reads in 227.448 CPU sec, 7.289 real sec
[M::mem_process_seqs] Processed 676966 reads in 126.808 CPU sec, 4.035 real sec
sorting block 43, #reads = 1082823 mem = 536879402 peak = 952909824
[M::process] read 676746 sequences (100000145 bp) in 3.119 CPU sec, 3.459 real sec...
[M::process] read 676692 sequences (100000172 bp) in 2.109 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287828, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.50, 112.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676928 reads in 129.506 CPU sec, 4.150 real sec
sorting block 44, #reads = 1082147 mem = 536879884 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288184, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.21, 111.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287814, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.59, 111.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677380 sequences (100000059 bp) in 2.001 CPU sec, 2.034 real sec...
[M::mem_process_seqs] Processed 677012 reads in 352.386 CPU sec, 11.343 real sec
[M::mem_process_seqs] Processed 676746 reads in 141.835 CPU sec, 4.581 real sec
sorting block 45, #reads = 1082647 mem = 536878408 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288293, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.18, 114.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677674 sequences (100000207 bp) in 3.307 CPU sec, 3.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288073, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (218.54, 100.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677644 sequences (100000065 bp) in 1.963 CPU sec, 1.971 real sec...
[M::mem_process_seqs] Processed 676692 reads in 229.183 CPU sec, 7.347 real sec
[M::mem_process_seqs] Processed 677380 reads in 129.464 CPU sec, 4.125 real sec
sorting block 46, #reads = 1082844 mem = 536880345 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288734, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (211.42, 89.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 16, 76)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196)
[M::mem_pestat] mean and std.dev: (29.38, 30.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 256)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677446 sequences (100000092 bp) in 3.253 CPU sec, 3.548 real sec...
[M::process] read 677328 sequences (100000215 bp) in 1.960 CPU sec, 1.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288452, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (219.19, 100.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677674 reads in 223.744 CPU sec, 7.160 real sec
[M::mem_process_seqs] Processed 677644 reads in 126.536 CPU sec, 4.035 real sec
sorting block 47, #reads = 1081986 mem = 536881007 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288580, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (219.93, 101.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1082774 mem = 536878108 peak = 952909824
[M::process] read 677160 sequences (100000207 bp) in 2.807 CPU sec, 3.098 real sec...
[M::process] read 677096 sequences (100000211 bp) in 1.931 CPU sec, 1.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288665, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.11, 102.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677446 reads in 219.855 CPU sec, 7.045 real sec
[M::mem_process_seqs] Processed 677328 reads in 121.780 CPU sec, 3.863 real sec
sorting block 49, #reads = 1083075 mem = 536880078 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288008, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.33, 107.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676966 sequences (100000280 bp) in 2.488 CPU sec, 2.716 real sec...
[M::process] read 676896 sequences (100000076 bp) in 2.018 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288079, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.48, 109.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677160 reads in 215.879 CPU sec, 6.887 real sec
[M::mem_process_seqs] Processed 677096 reads in 119.779 CPU sec, 3.810 real sec
sorting block 50, #reads = 1082387 mem = 536879762 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287924, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.47, 110.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676774 sequences (100000178 bp) in 3.130 CPU sec, 3.444 real sec...
[M::process] read 676696 sequences (100000009 bp) in 1.982 CPU sec, 1.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288201, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.26, 111.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676966 reads in 226.403 CPU sec, 7.254 real sec
[M::mem_process_seqs] Processed 676896 reads in 127.937 CPU sec, 4.070 real sec
sorting block 51, #reads = 1082240 mem = 536878500 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288189, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.84, 113.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676678 sequences (100000213 bp) in 3.597 CPU sec, 3.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287862, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.82, 114.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 89, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (166.40, 143.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676774 reads in 223.005 CPU sec, 7.138 real sec
[M::process] read 677266 sequences (100000046 bp) in 1.939 CPU sec, 1.962 real sec...
[M::mem_process_seqs] Processed 676696 reads in 126.393 CPU sec, 4.011 real sec
sorting block 52, #reads = 1082797 mem = 536878184 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287847, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.12, 116.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1082499 mem = 536880840 peak = 952909824
[M::process] read 677760 sequences (100000205 bp) in 3.049 CPU sec, 3.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288511, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.21, 108.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677796 sequences (100000062 bp) in 2.107 CPU sec, 2.112 real sec...
[M::mem_process_seqs] Processed 676678 reads in 220.989 CPU sec, 7.076 real sec
[M::mem_process_seqs] Processed 677266 reads in 123.295 CPU sec, 3.904 real sec
sorting block 54, #reads = 1081770 mem = 536878307 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288387, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (212.47, 90.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677492 sequences (100000164 bp) in 2.963 CPU sec, 3.192 real sec...
[M::process] read 677398 sequences (100000145 bp) in 2.016 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288380, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (216.73, 96.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677760 reads in 224.724 CPU sec, 7.202 real sec
[M::mem_process_seqs] Processed 677796 reads in 126.078 CPU sec, 4.002 real sec
sorting block 55, #reads = 1082735 mem = 536881765 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288552, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.79, 99.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677028 sequences (100000060 bp) in 2.936 CPU sec, 3.237 real sec...
[M::process] read 676934 sequences (100000063 bp) in 1.984 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288541, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.98, 102.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677492 reads in 217.731 CPU sec, 6.974 real sec
[M::mem_process_seqs] Processed 677398 reads in 125.410 CPU sec, 3.989 real sec
sorting block 56, #reads = 1083014 mem = 536882315 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287977, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.65, 107.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676838 sequences (100000147 bp) in 2.789 CPU sec, 3.047 real sec...
[M::process] read 676802 sequences (100000178 bp) in 1.940 CPU sec, 1.946 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287932, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.98, 110.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677028 reads in 214.158 CPU sec, 6.835 real sec
sorting block 57, #reads = 1082949 mem = 536878706 peak = 952909824
[M::mem_process_seqs] Processed 676934 reads in 121.415 CPU sec, 3.871 real sec
sorting block 58, #reads = 1082815 mem = 536878355 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288260, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.64, 110.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676638 sequences (100000271 bp) in 3.131 CPU sec, 3.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287287, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.96, 112.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676566 sequences (100000133 bp) in 1.990 CPU sec, 1.995 real sec...
[M::mem_process_seqs] Processed 676838 reads in 218.611 CPU sec, 6.996 real sec
[M::mem_process_seqs] Processed 676802 reads in 121.393 CPU sec, 3.848 real sec
sorting block 59, #reads = 1082728 mem = 536877903 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287770, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.16, 114.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676508 sequences (100000142 bp) in 3.177 CPU sec, 3.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287997, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.94, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 659368 sequences (97461141 bp) in 2.010 CPU sec, 2.012 real sec...
[M::mem_process_seqs] Processed 676638 reads in 211.410 CPU sec, 6.745 real sec
[M::mem_process_seqs] Processed 676566 reads in 122.162 CPU sec, 3.878 real sec
sorting block 60, #reads = 1081684 mem = 536879134 peak = 952909824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287827, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.59, 117.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280304, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.69, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676508 reads in 206.967 CPU sec, 6.748 real sec
[M::mem_process_seqs] Processed 659368 reads in 114.855 CPU sec, 3.757 real sec
sorting block 61, #reads = 1082201 mem = 536878641 peak = 952909824
sorting block 62, #reads = 1082011 mem = 536880332 peak = 952909824
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67680224
overall: 69421498368 mem 13320.835 user 721.074 sys 501.637 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L007\tPU:L007\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T21-D0003376_R1.fastq.gz 0004.T21-D0003376_R2.fastq.gz
[main] Real time: 501.664 sec; CPU: 14041.914 sec
sorting block 63, #reads = 11293 mem = 5610451 peak = 952909824
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2645451547
output reads: 68206467
bam_mem_sort: 64 calls 49.092 user 1.086 sys 50.303 real
bam_write: 64 calls 33.262 user 2.543 sys 41.204 real
execute: 1 calls 18.267 user 1.733 sys 522.200 real
merge_files: 1 calls 18.259 user 1.697 sys 20.517 real
parse_chunk: 1009 calls 46.180 user 12.838 sys 60.895 real
read_chunk: 2716 calls 28.115 user 17.736 sys 389.391 real
sort_block: 1 calls 20.669 user 16.934 sys 376.645 real
write_chunk: 1705 calls 17.836 user 1.445 sys 19.668 real
overall: 2124185600 mem 710.792 user 57.598 sys 522.216 real
ls: cannot access '*.cram': No such file or directory