.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003376;L003;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a9/05b0140954499b79db6632e69613da/.command.log
Size: 91.1 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003376;L003;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a9/05b0140954499b79db6632e69613da/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bf/010bea11553f8e395b590fdf02ef4b/T21-D0003376/0001.T21-D0003376_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a9/05b0140954499b79db6632e69613da/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bf/010bea11553f8e395b590fdf02ef4b/T21-D0003376/0001.T21-D0003376_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2833.931 CPU sec, 114.353 real sec
[M::process] read 676990 sequences (100000077 bp) in 1.174 CPU sec, 1.183 real sec...
[M::process] read 676934 sequences (100000134 bp) in 1.634 CPU sec, 1.656 real sec...
[M::process] read 677028 sequences (100000106 bp) in 1.481 CPU sec, 1.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288614, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.23, 107.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677000 sequences (100000163 bp) in 1.459 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288849, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (226.23, 105.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 194, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142)
[M::mem_pestat] mean and std.dev: (274.14, 234.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1499)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288487, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.88, 106.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 655, 3458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10202)
[M::mem_pestat] mean and std.dev: (1542.10, 1579.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13574)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676990 reads in 317.658 CPU sec, 10.317 real sec
[M::mem_process_seqs] Processed 676934 reads in 222.025 CPU sec, 7.359 real sec
[M::mem_process_seqs] Processed 677028 reads in 232.237 CPU sec, 7.732 real sec
sorting block 0, #reads = 1082198 mem = 536879116 peak = 574439424
[M::process] read 676896 sequences (100000289 bp) in 2.513 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288044, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.66, 107.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676704 sequences (100000282 bp) in 2.250 CPU sec, 2.509 real sec...
[M::process] read 676838 sequences (100000267 bp) in 1.456 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287967, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.02, 110.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677000 reads in 227.908 CPU sec, 7.340 real sec
[M::mem_process_seqs] Processed 676896 reads in 128.094 CPU sec, 4.122 real sec
sorting block 1, #reads = 1082608 mem = 536880366 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288113, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.27, 109.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1082365 mem = 536879524 peak = 800931840
[M::process] read 676688 sequences (100000204 bp) in 3.201 CPU sec, 3.610 real sec...
[M::process] read 676686 sequences (100000141 bp) in 1.561 CPU sec, 1.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288011, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (226.90, 107.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676704 reads in 227.424 CPU sec, 7.327 real sec
[M::mem_process_seqs] Processed 676838 reads in 127.970 CPU sec, 4.163 real sec
sorting block 3, #reads = 1082420 mem = 536879177 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287508, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.48, 111.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676678 sequences (100000099 bp) in 2.566 CPU sec, 2.934 real sec...
[M::process] read 676224 sequences (100000023 bp) in 1.481 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288036, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.28, 113.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676688 reads in 232.212 CPU sec, 7.510 real sec
[M::mem_process_seqs] Processed 676686 reads in 131.067 CPU sec, 4.216 real sec
sorting block 4, #reads = 1081854 mem = 536879768 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287529, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.08, 114.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676192 sequences (100000197 bp) in 2.178 CPU sec, 2.374 real sec...
[M::process] read 676290 sequences (100000212 bp) in 1.481 CPU sec, 1.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288131, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.04, 116.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676678 reads in 230.635 CPU sec, 7.461 real sec
[M::mem_process_seqs] Processed 676224 reads in 129.516 CPU sec, 4.177 real sec
sorting block 5, #reads = 1082588 mem = 536878229 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287507, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (238.70, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676946 sequences (100000150 bp) in 2.437 CPU sec, 2.722 real sec...
[M::process] read 677030 sequences (100000092 bp) in 1.435 CPU sec, 1.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287894, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.85, 118.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676192 reads in 229.796 CPU sec, 7.425 real sec
sorting block 6, #reads = 1082437 mem = 536879452 peak = 800931840
[M::mem_process_seqs] Processed 676290 reads in 127.985 CPU sec, 4.145 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288388, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.96, 110.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1082043 mem = 536877290 peak = 800931840
[M::process] read 677074 sequences (100000018 bp) in 2.616 CPU sec, 2.935 real sec...
[M::process] read 676846 sequences (100000120 bp) in 1.441 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289165, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.03, 107.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676946 reads in 227.646 CPU sec, 7.364 real sec
[M::mem_process_seqs] Processed 677030 reads in 129.373 CPU sec, 4.179 real sec
sorting block 8, #reads = 1082464 mem = 536879433 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288552, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.01, 107.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 89, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2082)
[M::mem_pestat] mean and std.dev: (332.00, 340.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2761)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676898 sequences (100000065 bp) in 2.458 CPU sec, 2.767 real sec...
[M::process] read 676954 sequences (100000059 bp) in 1.470 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288077, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.55, 110.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 78, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (180.90, 232.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677074 reads in 232.291 CPU sec, 7.511 real sec
[M::mem_process_seqs] Processed 676846 reads in 130.228 CPU sec, 4.183 real sec
sorting block 9, #reads = 1082617 mem = 536882295 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288589, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.99, 110.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676972 sequences (100000258 bp) in 2.773 CPU sec, 3.102 real sec...
[M::process] read 676800 sequences (100000108 bp) in 1.682 CPU sec, 1.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288366, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.20, 109.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676898 reads in 234.723 CPU sec, 7.579 real sec
[M::mem_process_seqs] Processed 676954 reads in 131.163 CPU sec, 4.210 real sec
sorting block 10, #reads = 1082664 mem = 536879378 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288644, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.26, 108.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676574 sequences (100000020 bp) in 2.436 CPU sec, 2.767 real sec...
[M::process] read 676484 sequences (100000165 bp) in 1.347 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288106, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.94, 112.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676972 reads in 235.570 CPU sec, 7.639 real sec
sorting block 11, #reads = 1082579 mem = 536877038 peak = 800931840
[M::mem_process_seqs] Processed 676800 reads in 131.688 CPU sec, 4.262 real sec
sorting block 12, #reads = 1082825 mem = 536881853 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287664, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.44, 113.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676382 sequences (100000299 bp) in 2.678 CPU sec, 2.993 real sec...
[M::process] read 676246 sequences (100000025 bp) in 1.610 CPU sec, 1.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287860, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.25, 114.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676574 reads in 241.091 CPU sec, 7.849 real sec
[M::mem_process_seqs] Processed 676484 reads in 133.665 CPU sec, 4.359 real sec
sorting block 13, #reads = 1082334 mem = 536879541 peak = 800931840
[M::process] read 676620 sequences (100000182 bp) in 2.697 CPU sec, 3.095 real sec...
[M::process] read 676776 sequences (100000174 bp) in 1.559 CPU sec, 1.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287792, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.30, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676246 reads in 125.515 CPU sec, 4.046 real sec
sorting block 14, #reads = 1082225 mem = 536879343 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288140, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.02, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 334, 1124)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3332)
[M::mem_pestat] mean and std.dev: (431.64, 483.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4436)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677088 sequences (100000192 bp) in 1.931 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288339, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.36, 113.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676382 reads in 361.592 CPU sec, 11.851 real sec
[M::mem_process_seqs] Processed 676620 reads in 143.389 CPU sec, 4.768 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288212, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.25, 112.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1082437 mem = 536877983 peak = 800931840
[M::process] read 676974 sequences (100000042 bp) in 2.911 CPU sec, 3.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288274, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.36, 107.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676792 sequences (100000085 bp) in 1.492 CPU sec, 1.497 real sec...
[M::mem_process_seqs] Processed 676776 reads in 234.578 CPU sec, 7.669 real sec
[M::mem_process_seqs] Processed 677088 reads in 132.451 CPU sec, 4.288 real sec
sorting block 16, #reads = 1082226 mem = 536879638 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288551, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.22, 107.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 266, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (287.82, 236.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676820 sequences (100000247 bp) in 2.630 CPU sec, 2.991 real sec...
[M::process] read 676996 sequences (100000291 bp) in 1.542 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288241, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.90, 111.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676974 reads in 237.936 CPU sec, 7.793 real sec
[M::mem_process_seqs] Processed 676792 reads in 132.463 CPU sec, 4.357 real sec
sorting block 17, #reads = 1082697 mem = 536879426 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288866, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.30, 111.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676756 sequences (100000014 bp) in 2.480 CPU sec, 2.780 real sec...
[M::process] read 676650 sequences (100000103 bp) in 1.466 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288607, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.53, 106.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676820 reads in 235.803 CPU sec, 7.695 real sec
[M::mem_process_seqs] Processed 676996 reads in 129.445 CPU sec, 4.184 real sec
sorting block 18, #reads = 1082613 mem = 536878063 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288165, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.48, 110.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 366, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805)
[M::mem_pestat] mean and std.dev: (345.08, 296.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2398)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 19, #reads = 1082633 mem = 536877928 peak = 800931840
[M::process] read 676598 sequences (100000069 bp) in 3.180 CPU sec, 3.525 real sec...
[M::process] read 676452 sequences (100000121 bp) in 1.712 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288309, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.66, 112.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 13, 945)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2821)
[M::mem_pestat] mean and std.dev: (234.80, 371.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3759)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676756 reads in 244.592 CPU sec, 8.015 real sec
[M::mem_process_seqs] Processed 676650 reads in 139.474 CPU sec, 4.567 real sec
sorting block 20, #reads = 1082389 mem = 536881469 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288268, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.83, 114.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676508 sequences (100000262 bp) in 2.493 CPU sec, 2.837 real sec...
[M::process] read 676202 sequences (100000045 bp) in 1.473 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288081, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.46, 117.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676598 reads in 243.019 CPU sec, 8.060 real sec
[M::mem_process_seqs] Processed 676452 reads in 136.204 CPU sec, 4.515 real sec
sorting block 21, #reads = 1082076 mem = 536877671 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287527, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.62, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676840 sequences (100000007 bp) in 2.484 CPU sec, 2.804 real sec...
[M::process] read 676558 sequences (100000199 bp) in 1.462 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287944, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.50, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676508 reads in 243.452 CPU sec, 8.018 real sec
[M::mem_process_seqs] Processed 676202 reads in 136.473 CPU sec, 4.504 real sec
sorting block 22, #reads = 1082631 mem = 536880560 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288385, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.98, 108.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676844 sequences (100000206 bp) in 3.163 CPU sec, 3.589 real sec...
[M::process] read 676918 sequences (100000189 bp) in 1.412 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288223, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.10, 112.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676840 reads in 234.665 CPU sec, 7.743 real sec
sorting block 23, #reads = 1081315 mem = 536878410 peak = 800931840
[M::mem_process_seqs] Processed 676558 reads in 131.747 CPU sec, 4.400 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288467, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.58, 108.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1082372 mem = 536881085 peak = 800931840
[M::process] read 676768 sequences (100000169 bp) in 2.849 CPU sec, 3.260 real sec...
[M::process] read 676742 sequences (100000092 bp) in 1.566 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288692, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.62, 109.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676844 reads in 234.395 CPU sec, 7.689 real sec
[M::mem_process_seqs] Processed 676918 reads in 130.685 CPU sec, 4.297 real sec
sorting block 25, #reads = 1082245 mem = 536879390 peak = 800931840
[M::process] read 676792 sequences (100000153 bp) in 2.449 CPU sec, 2.789 real sec...
[M::process] read 676746 sequences (100000197 bp) in 1.411 CPU sec, 1.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288363, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.57, 110.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676742 reads in 124.495 CPU sec, 4.170 real sec
sorting block 26, #reads = 1082092 mem = 536879896 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288850, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.14, 110.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676672 sequences (100000242 bp) in 1.617 CPU sec, 1.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288318, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.97, 107.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676768 reads in 362.068 CPU sec, 11.942 real sec
[M::mem_process_seqs] Processed 676792 reads in 142.214 CPU sec, 4.687 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288304, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.24, 111.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 119, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334)
[M::mem_pestat] mean and std.dev: (249.00, 213.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1750)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 27, #reads = 1082399 mem = 536881336 peak = 800931840
[M::process] read 676518 sequences (100000134 bp) in 3.678 CPU sec, 4.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287775, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.01, 112.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676746 reads in 238.457 CPU sec, 7.750 real sec
[M::mem_process_seqs] Processed 676672 reads in 133.251 CPU sec, 4.305 real sec
[M::process] read 676400 sequences (100000173 bp) in 1.843 CPU sec, 1.982 real sec...
sorting block 28, #reads = 1082938 mem = 536878900 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288266, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.83, 114.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676270 sequences (100000055 bp) in 2.682 CPU sec, 3.245 real sec...
[M::process] read 676296 sequences (100000065 bp) in 1.548 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287667, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.80, 115.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676518 reads in 228.081 CPU sec, 7.442 real sec
[M::mem_process_seqs] Processed 676400 reads in 124.143 CPU sec, 4.056 real sec
sorting block 29, #reads = 1082623 mem = 536879668 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287874, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.41, 117.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677306 sequences (100000251 bp) in 2.612 CPU sec, 2.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287848, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.98, 118.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677348 sequences (100000281 bp) in 1.484 CPU sec, 1.557 real sec...
[M::mem_process_seqs] Processed 676270 reads in 214.212 CPU sec, 6.988 real sec
[M::mem_process_seqs] Processed 676296 reads in 122.035 CPU sec, 3.952 real sec
sorting block 30, #reads = 1081624 mem = 536877175 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288060, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (222.32, 104.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1081874 mem = 536880672 peak = 800931840
[M::process] read 677220 sequences (100000124 bp) in 2.764 CPU sec, 3.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288399, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.92, 105.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677248 sequences (100000079 bp) in 1.460 CPU sec, 1.521 real sec...
[M::mem_process_seqs] Processed 677306 reads in 216.659 CPU sec, 7.070 real sec
[M::mem_process_seqs] Processed 677348 reads in 123.982 CPU sec, 4.048 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288524, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.95, 107.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1081762 mem = 536880521 peak = 800931840
[M::process] read 677236 sequences (100000071 bp) in 3.465 CPU sec, 4.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288449, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.09, 109.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677220 reads in 219.526 CPU sec, 7.140 real sec
[M::mem_process_seqs] Processed 677248 reads in 125.453 CPU sec, 4.047 real sec
[M::process] read 677008 sequences (100000243 bp) in 1.679 CPU sec, 1.745 real sec...
sorting block 33, #reads = 1081988 mem = 536878816 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287878, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.37, 108.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 34, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (94.09, 96.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676906 sequences (100000257 bp) in 2.482 CPU sec, 2.936 real sec...
[M::process] read 676994 sequences (100000008 bp) in 1.506 CPU sec, 1.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287977, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.05, 108.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677236 reads in 218.560 CPU sec, 7.094 real sec
[M::mem_process_seqs] Processed 677008 reads in 125.336 CPU sec, 4.025 real sec
sorting block 34, #reads = 1079975 mem = 536881953 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287620, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.23, 108.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180)
[M::mem_pestat] mean and std.dev: (303.45, 231.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1513)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676842 sequences (100000091 bp) in 3.067 CPU sec, 3.365 real sec...
[M::process] read 676972 sequences (100000231 bp) in 1.411 CPU sec, 1.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287283, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.54, 109.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 30, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257)
[M::mem_pestat] mean and std.dev: (48.82, 50.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 341)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676906 reads in 222.002 CPU sec, 7.174 real sec
[M::mem_process_seqs] Processed 676994 reads in 124.900 CPU sec, 4.043 real sec
sorting block 35, #reads = 1080675 mem = 536878063 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287299, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.43, 111.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1082262 mem = 536879043 peak = 800931840
[M::process] read 676590 sequences (100000213 bp) in 2.869 CPU sec, 3.309 real sec...
[M::process] read 676502 sequences (100000023 bp) in 1.492 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287610, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.33, 111.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676842 reads in 227.481 CPU sec, 7.414 real sec
[M::mem_process_seqs] Processed 676972 reads in 127.595 CPU sec, 4.175 real sec
sorting block 37, #reads = 1082425 mem = 536879194 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287537, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.38, 114.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677082 sequences (100000054 bp) in 2.955 CPU sec, 3.320 real sec...
[M::process] read 677382 sequences (100000228 bp) in 1.464 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287367, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.05, 116.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676590 reads in 225.961 CPU sec, 7.352 real sec
[M::mem_process_seqs] Processed 676502 reads in 125.825 CPU sec, 4.118 real sec
sorting block 38, #reads = 1082372 mem = 536878987 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288391, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.04, 109.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 79, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499)
[M::mem_pestat] mean and std.dev: (226.14, 248.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1975)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677240 sequences (100000064 bp) in 2.480 CPU sec, 2.791 real sec...
[M::process] read 677096 sequences (100000172 bp) in 1.433 CPU sec, 1.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288365, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.57, 106.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677082 reads in 214.481 CPU sec, 6.972 real sec
sorting block 39, #reads = 1082425 mem = 536879419 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288850, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.21, 105.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677042 sequences (100000298 bp) in 1.921 CPU sec, 1.945 real sec...
[M::mem_process_seqs] Processed 677240 reads in 113.108 CPU sec, 3.634 real sec
[M::mem_process_seqs] Processed 677382 reads in 301.679 CPU sec, 9.798 real sec
sorting block 40, #reads = 1082979 mem = 536879958 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288562, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.42, 108.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677030 sequences (100000118 bp) in 2.489 CPU sec, 2.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288170, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.63, 110.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 21, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192)
[M::mem_pestat] mean and std.dev: (58.30, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1584)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677096 reads in 228.706 CPU sec, 7.418 real sec
[M::process] read 676874 sequences (100000139 bp) in 1.522 CPU sec, 1.561 real sec...
[M::mem_process_seqs] Processed 677042 reads in 126.884 CPU sec, 4.154 real sec
sorting block 41, #reads = 1080830 mem = 536879899 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288240, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.78, 109.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676984 sequences (100000209 bp) in 2.271 CPU sec, 2.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288122, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.53, 105.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677164 sequences (100000062 bp) in 2.133 CPU sec, 2.173 real sec...
[M::mem_process_seqs] Processed 677030 reads in 220.757 CPU sec, 7.178 real sec
[M::mem_process_seqs] Processed 676874 reads in 122.924 CPU sec, 3.964 real sec
sorting block 42, #reads = 1077570 mem = 536879017 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287789, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.07, 108.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1080232 mem = 536881792 peak = 800931840
[M::process] read 676914 sequences (100000222 bp) in 2.804 CPU sec, 3.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287913, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.74, 109.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676708 sequences (100000119 bp) in 1.442 CPU sec, 1.473 real sec...
[M::mem_process_seqs] Processed 676984 reads in 220.065 CPU sec, 7.174 real sec
[M::mem_process_seqs] Processed 677164 reads in 124.469 CPU sec, 4.050 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288029, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.52, 109.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 78, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (111.30, 94.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 44, #reads = 1081791 mem = 536878131 peak = 800931840
[M::process] read 676816 sequences (100000181 bp) in 3.250 CPU sec, 3.649 real sec...
[M::process] read 676978 sequences (100000036 bp) in 1.656 CPU sec, 1.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287564, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.72, 112.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676914 reads in 224.300 CPU sec, 7.231 real sec
[M::mem_process_seqs] Processed 676708 reads in 126.613 CPU sec, 4.045 real sec
sorting block 45, #reads = 1081149 mem = 536878394 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287425, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.47, 112.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677132 sequences (100000178 bp) in 2.302 CPU sec, 2.795 real sec...
[M::process] read 677184 sequences (100000143 bp) in 1.461 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288109, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.62, 111.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (82, 125, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1699)
[M::mem_pestat] mean and std.dev: (294.36, 278.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2238)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676816 reads in 226.235 CPU sec, 7.373 real sec
[M::mem_process_seqs] Processed 676978 reads in 127.024 CPU sec, 4.111 real sec
sorting block 46, #reads = 1081567 mem = 536878143 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288528, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.99, 109.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 33, 994)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2952)
[M::mem_pestat] mean and std.dev: (451.23, 599.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3931)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677086 sequences (100000137 bp) in 2.531 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288039, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.35, 108.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 23, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300)
[M::mem_pestat] mean and std.dev: (40.07, 42.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 399)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677058 sequences (100000257 bp) in 1.477 CPU sec, 1.533 real sec...
[M::mem_process_seqs] Processed 677132 reads in 220.516 CPU sec, 7.228 real sec
[M::mem_process_seqs] Processed 677184 reads in 125.329 CPU sec, 4.116 real sec
sorting block 47, #reads = 1079201 mem = 536881216 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288614, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.73, 107.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1082190 mem = 536879211 peak = 800931840
[M::process] read 676924 sequences (100000009 bp) in 2.971 CPU sec, 3.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287916, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.06, 110.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677086 reads in 206.625 CPU sec, 6.689 real sec
[M::mem_process_seqs] Processed 677058 reads in 118.214 CPU sec, 3.777 real sec
[M::process] read 677070 sequences (100000212 bp) in 1.978 CPU sec, 2.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287841, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.82, 111.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1082018 mem = 536877556 peak = 800931840
[M::process] read 677048 sequences (100000196 bp) in 2.742 CPU sec, 3.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287849, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.68, 106.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 340, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (153.22, 167.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1351)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676924 reads in 205.128 CPU sec, 6.743 real sec
[M::mem_process_seqs] Processed 677070 reads in 114.588 CPU sec, 3.803 real sec
[M::process] read 676994 sequences (100000125 bp) in 2.479 CPU sec, 2.819 real sec...
sorting block 50, #reads = 1077218 mem = 536881679 peak = 800931840
[M::process] read 676820 sequences (100000098 bp) in 2.207 CPU sec, 2.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287901, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.97, 109.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676994 reads in 115.743 CPU sec, 3.749 real sec
sorting block 51, #reads = 1080820 mem = 536880548 peak = 800931840
[M::process] read 676918 sequences (100000218 bp) in 2.614 CPU sec, 2.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288100, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.95, 105.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 262, 2072)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6058)
[M::mem_pestat] mean and std.dev: (733.09, 953.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8051)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287605, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.87, 109.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 14, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538)
[M::mem_pestat] mean and std.dev: (93.46, 182.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2046)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677048 reads in 328.817 CPU sec, 10.700 real sec
[M::mem_process_seqs] Processed 676820 reads in 133.584 CPU sec, 4.311 real sec
[M::process] read 676768 sequences (100000027 bp) in 2.754 CPU sec, 3.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287958, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.15, 110.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1082428 mem = 536879879 peak = 800931840
[M::process] read 676688 sequences (100000198 bp) in 2.577 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287708, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.10, 112.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676918 reads in 218.876 CPU sec, 7.208 real sec
[M::mem_process_seqs] Processed 676768 reads in 125.957 CPU sec, 4.219 real sec
sorting block 53, #reads = 1082290 mem = 536877835 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287647, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.58, 112.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676688 reads in 110.228 CPU sec, 3.717 real sec
[M::process] read 677140 sequences (100000152 bp) in 3.550 CPU sec, 4.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288028, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.81, 111.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677140 reads in 112.496 CPU sec, 3.797 real sec
[M::process] read 676924 sequences (100000121 bp) in 3.438 CPU sec, 4.019 real sec...
sorting block 54, #reads = 1080224 mem = 536880511 peak = 800931840
[M::process] read 677094 sequences (100000193 bp) in 2.925 CPU sec, 3.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287358, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.26, 113.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 125, 4564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13664)
[M::mem_pestat] mean and std.dev: (1442.70, 2046.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18214)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676924 reads in 113.614 CPU sec, 3.891 real sec
[M::process] read 677070 sequences (100000256 bp) in 2.765 CPU sec, 3.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287929, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.08, 108.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677094 reads in 109.934 CPU sec, 3.758 real sec
sorting block 55, #reads = 1080577 mem = 536877880 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288662, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.74, 108.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676788 sequences (100000295 bp) in 2.943 CPU sec, 3.395 real sec...
[M::mem_process_seqs] Processed 677070 reads in 109.146 CPU sec, 3.715 real sec
sorting block 56, #reads = 1082142 mem = 536879605 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288070, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.43, 111.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 46, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (35.79, 52.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676788 reads in 94.624 CPU sec, 3.162 real sec
[M::process] read 676884 sequences (100000293 bp) in 3.722 CPU sec, 4.327 real sec...
sorting block 57, #reads = 1082356 mem = 536877839 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288213, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.32, 109.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676884 reads in 93.223 CPU sec, 3.081 real sec
[M::process] read 676932 sequences (100000210 bp) in 3.103 CPU sec, 3.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288531, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.00, 107.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676932 reads in 104.637 CPU sec, 3.465 real sec
[M::process] read 677058 sequences (100000159 bp) in 3.464 CPU sec, 4.190 real sec...
sorting block 58, #reads = 1080753 mem = 536879929 peak = 800931840
[M::process] read 676878 sequences (100000046 bp) in 2.078 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287865, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.00, 109.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 125, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691)
[M::mem_pestat] mean and std.dev: (260.50, 323.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2242)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676902 sequences (100000185 bp) in 2.686 CPU sec, 2.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287687, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.06, 110.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 91, 9585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28675)
[M::mem_pestat] mean and std.dev: (2716.69, 4061.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38220)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677058 reads in 203.531 CPU sec, 6.541 real sec
[M::mem_process_seqs] Processed 676878 reads in 115.446 CPU sec, 3.686 real sec
sorting block 59, #reads = 1082877 mem = 536880605 peak = 800931840
[M::process] read 676668 sequences (100000070 bp) in 2.513 CPU sec, 2.779 real sec...
sorting block 60, #reads = 1082164 mem = 536880243 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287321, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.33, 113.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676668 reads in 103.721 CPU sec, 3.339 real sec
[M::process] read 676754 sequences (100000025 bp) in 3.828 CPU sec, 4.456 real sec...
[M::process] read 312020 sequences (46093628 bp) in 1.638 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287288, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.92, 111.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287979, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.87, 115.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676902 reads in 301.868 CPU sec, 10.541 real sec
[M::mem_process_seqs] Processed 676754 reads in 111.379 CPU sec, 3.597 real sec
sorting block 61, #reads = 1082075 mem = 536878020 peak = 800931840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 132839, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.65, 115.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 312020 reads in 48.067 CPU sec, 1.634 real sec
sorting block 62, #reads = 1082080 mem = 536878292 peak = 800931840
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67996234
overall: 69247414272 mem 13802.918 user 799.248 sys 529.672 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L003\tPU:L003\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T21-D0003376_R1.fastq.gz 0001.T21-D0003376_R2.fastq.gz
[main] Real time: 529.713 sec; CPU: 14602.180 sec
sorting block 63, #reads = 364319 mem = 180674299 peak = 800931840
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2729317430
output reads: 68521139
bam_mem_sort: 64 calls 59.787 user 2.602 sys 62.990 real
bam_write: 64 calls 44.394 user 4.795 sys 78.097 real
execute: 1 calls 16.812 user 0.942 sys 548.537 real
merge_files: 1 calls 16.782 user 0.918 sys 18.539 real
parse_chunk: 1014 calls 49.378 user 14.529 sys 72.160 real
read_chunk: 2730 calls 32.369 user 16.743 sys 414.024 real
sort_block: 1 calls 26.583 user 16.194 sys 404.106 real
write_chunk: 1714 calls 16.426 user 0.686 sys 17.792 real
overall: 2045779968 mem 766.992 user 57.900 sys 548.557 real
ls: cannot access '*.cram': No such file or directory