.command.err - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003376;L006;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1d/1196eb9383d028a79392922934a292/.command.err
Size: 89.5 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003376;L006;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2430.402 CPU sec, 98.557 real sec
[M::process] read 677676 sequences (100000143 bp) in 1.118 CPU sec, 1.121 real sec...
[M::process] read 677518 sequences (100000258 bp) in 1.655 CPU sec, 1.668 real sec...
[M::process] read 677224 sequences (100000156 bp) in 1.555 CPU sec, 1.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289462, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540)
[M::mem_pestat] mean and std.dev: (214.54, 92.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 666)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 34, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (131.23, 167.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1418)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677014 sequences (100000099 bp) in 1.519 CPU sec, 1.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289023, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (218.02, 97.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288740, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (221.18, 100.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677676 reads in 310.510 CPU sec, 10.174 real sec
[M::mem_process_seqs] Processed 677518 reads in 194.704 CPU sec, 6.523 real sec
[M::mem_process_seqs] Processed 677224 reads in 203.623 CPU sec, 6.861 real sec
sorting block 0, #reads = 1083073 mem = 536878812 peak = 549044224
[M::process] read 677072 sequences (100000045 bp) in 2.036 CPU sec, 2.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288599, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.63, 103.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 71, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232)
[M::mem_pestat] mean and std.dev: (207.83, 225.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1638)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676970 sequences (100000093 bp) in 1.493 CPU sec, 1.609 real sec...
[M::process] read 676854 sequences (100000020 bp) in 1.401 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288924, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.23, 107.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677014 reads in 189.783 CPU sec, 6.078 real sec
sorting block 1, #reads = 1082386 mem = 536881885 peak = 810139648
[M::process] read 676702 sequences (100000101 bp) in 1.456 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288492, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.45, 108.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676970 reads in 98.286 CPU sec, 3.156 real sec
sorting block 2, #reads = 1082567 mem = 536880204 peak = 810139648
[M::mem_process_seqs] Processed 677072 reads in 239.574 CPU sec, 7.696 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288640, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.44, 109.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676584 sequences (100000018 bp) in 2.190 CPU sec, 2.409 real sec...
[M::process] read 676510 sequences (100000187 bp) in 1.506 CPU sec, 1.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288175, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.90, 111.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 75, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (133.00, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676854 reads in 202.054 CPU sec, 6.482 real sec
[M::mem_process_seqs] Processed 676702 reads in 109.533 CPU sec, 3.510 real sec
sorting block 3, #reads = 1082679 mem = 536879777 peak = 815357952
[M::process] read 676518 sequences (100000123 bp) in 1.882 CPU sec, 2.027 real sec...
sorting block 4, #reads = 1082586 mem = 536879778 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288293, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.46, 111.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676438 sequences (100000203 bp) in 1.515 CPU sec, 1.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288089, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.53, 115.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 415, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (230.38, 187.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1393)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288342, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.56, 114.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676584 reads in 275.620 CPU sec, 8.907 real sec
[M::mem_process_seqs] Processed 676518 reads in 193.442 CPU sec, 6.292 real sec
[M::mem_process_seqs] Processed 676510 reads in 202.979 CPU sec, 6.658 real sec
sorting block 5, #reads = 1082098 mem = 536881560 peak = 921530368
[M::process] read 676896 sequences (100000053 bp) in 2.364 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288707, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.66, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677568 sequences (100000186 bp) in 2.059 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288499, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.50, 112.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 38, 187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (57.94, 100.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 715)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677512 sequences (100000274 bp) in 1.430 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 676438 reads in 197.926 CPU sec, 6.402 real sec
sorting block 6, #reads = 1082069 mem = 536877946 peak = 921530368
[M::mem_process_seqs] Processed 676896 reads in 113.376 CPU sec, 3.641 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288427, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.93, 98.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1082247 mem = 536880916 peak = 921530368
[M::process] read 677308 sequences (100000151 bp) in 2.109 CPU sec, 2.336 real sec...
[M::process] read 676982 sequences (100000240 bp) in 1.416 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288672, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.76, 102.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677568 reads in 193.536 CPU sec, 6.254 real sec
[M::mem_process_seqs] Processed 677512 reads in 112.013 CPU sec, 3.640 real sec
sorting block 8, #reads = 1082759 mem = 536876089 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288572, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.63, 103.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676886 sequences (100000242 bp) in 1.862 CPU sec, 2.064 real sec...
[M::process] read 676890 sequences (100000044 bp) in 1.335 CPU sec, 1.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288685, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.39, 107.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 58, 1603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4803)
[M::mem_pestat] mean and std.dev: (1002.60, 1627.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7511)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677308 reads in 197.633 CPU sec, 6.399 real sec
[M::mem_process_seqs] Processed 676982 reads in 113.177 CPU sec, 3.676 real sec
sorting block 9, #reads = 1083452 mem = 536878112 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288173, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.54, 108.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676804 sequences (100000084 bp) in 1.950 CPU sec, 2.228 real sec...
[M::process] read 676582 sequences (100000204 bp) in 1.408 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288375, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.38, 108.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676886 reads in 198.733 CPU sec, 6.417 real sec
[M::mem_process_seqs] Processed 676890 reads in 111.643 CPU sec, 3.615 real sec
sorting block 10, #reads = 1082797 mem = 536877818 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288304, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.24, 110.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 784, 3486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10276)
[M::mem_pestat] mean and std.dev: (1190.17, 1391.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13671)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676628 sequences (100000059 bp) in 2.412 CPU sec, 2.727 real sec...
[M::process] read 676478 sequences (100000148 bp) in 1.659 CPU sec, 1.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288316, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.40, 113.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676804 reads in 198.816 CPU sec, 6.686 real sec
sorting block 11, #reads = 1082375 mem = 536878719 peak = 921530368
[M::mem_process_seqs] Processed 676582 reads in 112.594 CPU sec, 3.801 real sec
sorting block 12, #reads = 1082456 mem = 536880092 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288145, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.20, 113.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676358 sequences (100000298 bp) in 2.054 CPU sec, 2.217 real sec...
[M::process] read 676270 sequences (100000191 bp) in 1.535 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288521, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.53, 116.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676628 reads in 194.552 CPU sec, 6.274 real sec
[M::mem_process_seqs] Processed 676478 reads in 111.536 CPU sec, 3.610 real sec
sorting block 13, #reads = 1082490 mem = 536878604 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288312, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.61, 118.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677330 sequences (100000288 bp) in 2.149 CPU sec, 2.397 real sec...
[M::process] read 677466 sequences (100000153 bp) in 1.522 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287723, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.43, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676358 reads in 193.980 CPU sec, 6.248 real sec
[M::mem_process_seqs] Processed 676270 reads in 112.007 CPU sec, 3.602 real sec
sorting block 14, #reads = 1082486 mem = 536880564 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288408, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.20, 105.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677316 sequences (100000222 bp) in 2.178 CPU sec, 2.353 real sec...
[M::process] read 677170 sequences (100000239 bp) in 1.471 CPU sec, 1.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288311, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.43, 99.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677330 reads in 193.808 CPU sec, 6.252 real sec
[M::mem_process_seqs] Processed 677466 reads in 112.686 CPU sec, 3.641 real sec
sorting block 15, #reads = 1081080 mem = 536878175 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288657, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.53, 102.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1082388 mem = 536879582 peak = 921530368
[M::process] read 677056 sequences (100000135 bp) in 2.194 CPU sec, 2.405 real sec...
[M::process] read 676822 sequences (100000238 bp) in 1.422 CPU sec, 1.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288349, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.49, 104.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677316 reads in 197.798 CPU sec, 6.397 real sec
[M::mem_process_seqs] Processed 677170 reads in 114.404 CPU sec, 3.718 real sec
sorting block 17, #reads = 1082760 mem = 536880570 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288434, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.08, 107.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676970 sequences (100000229 bp) in 2.259 CPU sec, 2.543 real sec...
[M::process] read 676688 sequences (100000078 bp) in 1.568 CPU sec, 1.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287578, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.08, 108.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677056 reads in 205.377 CPU sec, 6.650 real sec
[M::mem_process_seqs] Processed 676822 reads in 119.488 CPU sec, 3.873 real sec
sorting block 18, #reads = 1082308 mem = 536880223 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288348, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.16, 109.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676800 sequences (100000056 bp) in 3.146 CPU sec, 3.617 real sec...
[M::process] read 676652 sequences (100000143 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287872, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.38, 111.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676970 reads in 204.963 CPU sec, 6.631 real sec
[M::mem_process_seqs] Processed 676688 reads in 120.945 CPU sec, 3.924 real sec
sorting block 19, #reads = 1082431 mem = 536877477 peak = 921530368
[M::process] read 676486 sequences (100000105 bp) in 2.282 CPU sec, 2.653 real sec...
[M::process] read 676294 sequences (100000094 bp) in 1.559 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288189, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.45, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676652 reads in 110.461 CPU sec, 3.530 real sec
sorting block 20, #reads = 1082362 mem = 536877156 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288156, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.49, 114.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 78, 187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (97.50, 75.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676414 sequences (100000154 bp) in 1.468 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287677, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.53, 117.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676800 reads in 312.064 CPU sec, 10.104 real sec
[M::mem_process_seqs] Processed 676486 reads in 129.131 CPU sec, 4.258 real sec
sorting block 21, #reads = 1082453 mem = 536876747 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287664, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.23, 119.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677488 sequences (100000272 bp) in 2.122 CPU sec, 2.347 real sec...
[M::process] read 677550 sequences (100000175 bp) in 1.463 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288088, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.48, 116.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 34, 56)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (44.64, 42.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 215)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676294 reads in 201.615 CPU sec, 6.504 real sec
[M::mem_process_seqs] Processed 676414 reads in 115.506 CPU sec, 3.756 real sec
sorting block 22, #reads = 1081661 mem = 536879119 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289072, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (217.44, 96.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 131, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1705)
[M::mem_pestat] mean and std.dev: (261.09, 288.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2247)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677280 sequences (100000277 bp) in 2.268 CPU sec, 2.479 real sec...
[M::process] read 676600 sequences (100000092 bp) in 1.544 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288812, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.35, 98.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677488 reads in 196.370 CPU sec, 6.325 real sec
sorting block 23, #reads = 1082080 mem = 536881250 peak = 921530368
[M::mem_process_seqs] Processed 677550 reads in 111.885 CPU sec, 3.623 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288539, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.35, 103.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1082882 mem = 536879260 peak = 921530368
[M::process] read 676998 sequences (100000008 bp) in 2.299 CPU sec, 2.578 real sec...
[M::process] read 676746 sequences (100000167 bp) in 1.481 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288101, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.73, 110.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 75, 184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500)
[M::mem_pestat] mean and std.dev: (92.42, 79.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 658)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677280 reads in 204.476 CPU sec, 6.639 real sec
[M::mem_process_seqs] Processed 676600 reads in 117.195 CPU sec, 3.836 real sec
sorting block 25, #reads = 1082938 mem = 536880052 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287875, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (226.73, 106.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676756 sequences (100000026 bp) in 2.402 CPU sec, 2.597 real sec...
[M::process] read 676680 sequences (100000136 bp) in 1.592 CPU sec, 1.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287652, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.17, 108.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676998 reads in 206.103 CPU sec, 6.659 real sec
[M::mem_process_seqs] Processed 676746 reads in 117.520 CPU sec, 3.818 real sec
sorting block 26, #reads = 1082327 mem = 536877303 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288598, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.00, 111.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676544 sequences (100000171 bp) in 2.531 CPU sec, 2.821 real sec...
[M::process] read 676578 sequences (100000013 bp) in 1.436 CPU sec, 1.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288380, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.78, 111.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676756 reads in 206.560 CPU sec, 6.685 real sec
[M::mem_process_seqs] Processed 676680 reads in 117.059 CPU sec, 3.820 real sec
sorting block 27, #reads = 1081830 mem = 536880249 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287927, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.86, 115.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 7, 12)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22)
[M::mem_pestat] mean and std.dev: (7.11, 2.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676196 sequences (100000166 bp) in 2.367 CPU sec, 2.585 real sec...
[M::process] read 676034 sequences (100000163 bp) in 1.678 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288464, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.88, 115.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676544 reads in 199.075 CPU sec, 6.424 real sec
sorting block 28, #reads = 1083058 mem = 536880031 peak = 921530368
[M::mem_process_seqs] Processed 676578 reads in 114.438 CPU sec, 3.685 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288303, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
sorting block 29, #reads = 1082330 mem = 536880036 peak = 921530368
[M::mem_pestat] mean and std.dev: (239.29, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677024 sequences (100000163 bp) in 2.285 CPU sec, 2.536 real sec...
[M::process] read 677902 sequences (100000075 bp) in 1.507 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287351, 22, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.79, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 33, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376)
[M::mem_pestat] mean and std.dev: (54.68, 76.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 499)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676196 reads in 199.401 CPU sec, 6.466 real sec
[M::mem_process_seqs] Processed 676034 reads in 112.706 CPU sec, 3.644 real sec
sorting block 30, #reads = 1082546 mem = 536876444 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288138, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.69, 111.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677756 sequences (100000150 bp) in 2.612 CPU sec, 2.966 real sec...
[M::process] read 677566 sequences (100000168 bp) in 1.522 CPU sec, 1.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288691, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (212.25, 90.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677024 reads in 203.725 CPU sec, 6.585 real sec
[M::mem_process_seqs] Processed 677902 reads in 115.965 CPU sec, 3.734 real sec
sorting block 31, #reads = 1081915 mem = 536880129 peak = 921530368
[M::process] read 677368 sequences (100000070 bp) in 2.364 CPU sec, 2.688 real sec...
[M::process] read 677316 sequences (100000190 bp) in 1.351 CPU sec, 1.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288561, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (217.26, 97.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677566 reads in 103.719 CPU sec, 3.324 real sec
sorting block 32, #reads = 1083078 mem = 536880065 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288395, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (216.06, 96.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288290, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.70, 102.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677158 sequences (100000230 bp) in 1.709 CPU sec, 1.798 real sec...
[M::mem_process_seqs] Processed 677756 reads in 308.867 CPU sec, 9.994 real sec
[M::mem_process_seqs] Processed 677368 reads in 127.702 CPU sec, 4.203 real sec
sorting block 33, #reads = 1082508 mem = 536878364 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288045, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (222.99, 104.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677040 sequences (100000193 bp) in 2.787 CPU sec, 3.122 real sec...
[M::process] read 676856 sequences (100000165 bp) in 1.221 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288498, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.17, 107.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 102, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1917)
[M::mem_pestat] mean and std.dev: (265.36, 371.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2548)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677316 reads in 206.145 CPU sec, 6.668 real sec
[M::mem_process_seqs] Processed 677158 reads in 117.173 CPU sec, 3.777 real sec
sorting block 34, #reads = 1082823 mem = 536880370 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287961, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.44, 108.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676862 sequences (100000140 bp) in 2.725 CPU sec, 3.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288433, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.16, 110.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676828 sequences (100000242 bp) in 1.605 CPU sec, 1.646 real sec...
[M::mem_process_seqs] Processed 677040 reads in 205.922 CPU sec, 6.653 real sec
sorting block 35, #reads = 1082732 mem = 536881029 peak = 921530368
[M::mem_process_seqs] Processed 676856 reads in 117.292 CPU sec, 3.774 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288308, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.87, 111.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 261, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605)
[M::mem_pestat] mean and std.dev: (359.46, 382.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2133)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 36, #reads = 1081741 mem = 536875932 peak = 921530368
[M::process] read 676796 sequences (100000240 bp) in 2.330 CPU sec, 2.864 real sec...
[M::process] read 676388 sequences (100000271 bp) in 1.472 CPU sec, 1.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288111, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.02, 113.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 316, 2089)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6213)
[M::mem_pestat] mean and std.dev: (836.88, 1030.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8275)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676862 reads in 206.915 CPU sec, 6.751 real sec
[M::mem_process_seqs] Processed 676828 reads in 117.533 CPU sec, 3.801 real sec
sorting block 37, #reads = 1082276 mem = 536882158 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288113, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.73, 115.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677876 sequences (100000141 bp) in 2.295 CPU sec, 2.604 real sec...
[M::process] read 677640 sequences (100000292 bp) in 1.512 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287703, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.66, 116.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 54, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1841)
[M::mem_pestat] mean and std.dev: (188.50, 256.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2443)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676796 reads in 202.396 CPU sec, 6.545 real sec
[M::mem_process_seqs] Processed 676388 reads in 114.515 CPU sec, 3.693 real sec
sorting block 38, #reads = 1082158 mem = 536877820 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288566, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (210.58, 88.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677510 sequences (100000275 bp) in 2.419 CPU sec, 3.054 real sec...
[M::process] read 677414 sequences (100000022 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288743, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (216.12, 96.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 573, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691)
[M::mem_pestat] mean and std.dev: (319.10, 300.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2250)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677876 reads in 200.430 CPU sec, 6.523 real sec
[M::mem_process_seqs] Processed 677640 reads in 112.555 CPU sec, 3.622 real sec
sorting block 39, #reads = 1082075 mem = 536877356 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288677, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.14, 100.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677296 sequences (100000214 bp) in 2.885 CPU sec, 3.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288679, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677278 sequences (100000034 bp) in 1.473 CPU sec, 1.480 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (221.01, 102.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677510 reads in 202.904 CPU sec, 6.571 real sec
sorting block 40, #reads = 1082938 mem = 536877273 peak = 921530368
[M::mem_process_seqs] Processed 677414 reads in 116.243 CPU sec, 3.744 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288397, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.06, 105.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1082812 mem = 536878520 peak = 921530368
[M::process] read 677160 sequences (100000156 bp) in 2.737 CPU sec, 3.110 real sec...
[M::process] read 676970 sequences (100000109 bp) in 1.613 CPU sec, 1.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287717, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.73, 104.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 51, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (151.00, 191.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677296 reads in 207.877 CPU sec, 6.749 real sec
[M::mem_process_seqs] Processed 677278 reads in 118.269 CPU sec, 3.861 real sec
sorting block 42, #reads = 1082430 mem = 536879920 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288369, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.35, 107.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676866 sequences (100000261 bp) in 2.463 CPU sec, 2.782 real sec...
[M::process] read 676874 sequences (100000194 bp) in 1.537 CPU sec, 1.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288070, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.72, 110.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677160 reads in 210.485 CPU sec, 6.844 real sec
[M::mem_process_seqs] Processed 676970 reads in 119.970 CPU sec, 3.919 real sec
sorting block 43, #reads = 1082142 mem = 536878846 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288073, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.73, 112.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676742 sequences (100000088 bp) in 2.798 CPU sec, 3.103 real sec...
[M::process] read 676624 sequences (100000267 bp) in 1.690 CPU sec, 1.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287553, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.31, 113.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 211, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565)
[M::mem_pestat] mean and std.dev: (99.12, 86.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676866 reads in 208.671 CPU sec, 6.711 real sec
[M::mem_process_seqs] Processed 676874 reads in 118.368 CPU sec, 3.815 real sec
sorting block 44, #reads = 1082012 mem = 536880284 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287563, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.04, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1082357 mem = 536878450 peak = 921530368
[M::process] read 677622 sequences (100000214 bp) in 2.523 CPU sec, 2.876 real sec...
[M::process] read 677638 sequences (100000201 bp) in 1.601 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287778, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.75, 115.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676742 reads in 210.922 CPU sec, 6.850 real sec
[M::mem_process_seqs] Processed 676624 reads in 118.519 CPU sec, 3.861 real sec
sorting block 46, #reads = 1082616 mem = 536877343 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288482, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (217.61, 98.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 121, 1859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5541)
[M::mem_pestat] mean and std.dev: (766.70, 892.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7382)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677654 sequences (100000024 bp) in 2.799 CPU sec, 3.236 real sec...
[M::process] read 677474 sequences (100000011 bp) in 1.673 CPU sec, 1.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288318, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (215.53, 94.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677622 reads in 204.632 CPU sec, 6.590 real sec
[M::mem_process_seqs] Processed 677638 reads in 117.355 CPU sec, 3.831 real sec
sorting block 47, #reads = 1082058 mem = 536878479 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288531, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.13, 99.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 11, 2051)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6139)
[M::mem_pestat] mean and std.dev: (736.00, 1177.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8183)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677208 sequences (100000146 bp) in 2.461 CPU sec, 2.771 real sec...
[M::process] read 677130 sequences (100000103 bp) in 1.643 CPU sec, 1.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288364, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.60, 103.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 226, 1350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4030)
[M::mem_pestat] mean and std.dev: (720.50, 893.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5370)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677654 reads in 206.252 CPU sec, 6.694 real sec
[M::mem_process_seqs] Processed 677474 reads in 116.626 CPU sec, 3.836 real sec
sorting block 48, #reads = 1082871 mem = 536877862 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288389, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (222.98, 105.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677188 sequences (100000239 bp) in 2.594 CPU sec, 2.920 real sec...
[M::process] read 677102 sequences (100000022 bp) in 1.460 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288341, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.58, 106.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 59, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (64.44, 55.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 647)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677208 reads in 207.360 CPU sec, 6.679 real sec
[M::mem_process_seqs] Processed 677130 reads in 116.925 CPU sec, 3.759 real sec
sorting block 49, #reads = 1083040 mem = 536877847 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287832, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.07, 106.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1082423 mem = 536878456 peak = 921530368
[M::process] read 676812 sequences (100000116 bp) in 2.902 CPU sec, 3.250 real sec...
[M::process] read 676744 sequences (100000271 bp) in 1.652 CPU sec, 1.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288184, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.47, 109.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677188 reads in 206.646 CPU sec, 6.669 real sec
[M::mem_process_seqs] Processed 677102 reads in 116.691 CPU sec, 3.780 real sec
sorting block 51, #reads = 1082637 mem = 536879068 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288233, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.14, 111.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676568 sequences (100000264 bp) in 2.679 CPU sec, 3.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288173, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.19, 113.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676530 sequences (100000130 bp) in 2.214 CPU sec, 2.230 real sec...
[M::mem_process_seqs] Processed 676812 reads in 213.127 CPU sec, 6.896 real sec
[M::mem_process_seqs] Processed 676744 reads in 121.726 CPU sec, 3.906 real sec
sorting block 52, #reads = 1082999 mem = 536877915 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287534, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.11, 114.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677216 sequences (100000099 bp) in 2.835 CPU sec, 3.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287847, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.33, 116.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677692 sequences (100000062 bp) in 1.985 CPU sec, 1.998 real sec...
[M::mem_process_seqs] Processed 676568 reads in 211.917 CPU sec, 6.824 real sec
[M::mem_process_seqs] Processed 676530 reads in 120.403 CPU sec, 3.847 real sec
sorting block 53, #reads = 1082313 mem = 536880057 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288608, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (222.78, 105.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677606 sequences (100000103 bp) in 2.401 CPU sec, 2.772 real sec...
[M::process] read 677166 sequences (100000132 bp) in 1.466 CPU sec, 1.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288291, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (213.58, 92.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677216 reads in 204.928 CPU sec, 6.653 real sec
[M::mem_process_seqs] Processed 677692 reads in 116.014 CPU sec, 3.763 real sec
sorting block 54, #reads = 1082228 mem = 536879470 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288230, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (216.52, 96.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1082521 mem = 536879601 peak = 921530368
[M::process] read 677336 sequences (100000115 bp) in 2.374 CPU sec, 2.742 real sec...
[M::process] read 677218 sequences (100000078 bp) in 1.847 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287742, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.00, 108.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677606 reads in 205.352 CPU sec, 6.633 real sec
[M::mem_process_seqs] Processed 677166 reads in 117.858 CPU sec, 3.802 real sec
sorting block 56, #reads = 1082770 mem = 536879308 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288247, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.49, 105.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 79, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (179.73, 194.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676882 sequences (100000157 bp) in 3.008 CPU sec, 3.443 real sec...
[M::process] read 676920 sequences (100000015 bp) in 1.674 CPU sec, 1.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288332, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.01, 108.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677336 reads in 205.213 CPU sec, 6.637 real sec
[M::mem_process_seqs] Processed 677218 reads in 118.532 CPU sec, 3.829 real sec
sorting block 57, #reads = 1082335 mem = 536879991 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287780, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.48, 110.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676740 sequences (100000246 bp) in 2.589 CPU sec, 2.832 real sec...
[M::process] read 676626 sequences (100000162 bp) in 1.580 CPU sec, 1.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288112, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.57, 109.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676882 reads in 205.236 CPU sec, 6.655 real sec
[M::mem_process_seqs] Processed 676920 reads in 116.497 CPU sec, 3.784 real sec
sorting block 58, #reads = 1082554 mem = 536876901 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287557, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.44, 113.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 41, 44)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 130)
[M::mem_pestat] mean and std.dev: (34.80, 32.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 173)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676540 sequences (100000187 bp) in 2.694 CPU sec, 2.960 real sec...
[M::process] read 676428 sequences (100000226 bp) in 1.474 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287390, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.06, 113.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676740 reads in 194.847 CPU sec, 6.267 real sec
[M::mem_process_seqs] Processed 676626 reads in 104.921 CPU sec, 3.355 real sec
sorting block 59, #reads = 1082573 mem = 536879455 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287551, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.28, 118.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1082162 mem = 536878811 peak = 921530368
[M::process] read 561694 sequences (83041487 bp) in 2.683 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287520, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (236.82, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676540 reads in 190.218 CPU sec, 6.135 real sec
[M::mem_process_seqs] Processed 676428 reads in 105.327 CPU sec, 3.403 real sec
sorting block 61, #reads = 1081337 mem = 536878317 peak = 921530368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 238739, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.50, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 561694 reads in 82.424 CPU sec, 2.764 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67584000
overall: 69279444992 mem 12237.198 user 758.313 sys 452.095 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L006\tPU:L006\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T21-D0003376_R1.fastq.gz 0001.T21-D0003376_R2.fastq.gz
[main] Real time: 452.128 sec; CPU: 12995.519 sec
sorting block 62, #reads = 999488 mem = 496078427 peak = 921530368
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2650643399
output reads: 68110876
bam_mem_sort: 63 calls 53.869 user 1.062 sys 56.021 real
bam_write: 63 calls 41.850 user 4.397 sys 79.416 real
execute: 1 calls 26.076 user 3.259 sys 484.362 real
merge_files: 1 calls 26.054 user 3.228 sys 31.203 real
parse_chunk: 1007 calls 48.244 user 14.827 sys 73.272 real
read_chunk: 2712 calls 32.611 user 14.822 sys 356.370 real
sort_block: 1 calls 24.332 user 13.597 sys 343.280 real
write_chunk: 1703 calls 25.425 user 2.980 sys 30.200 real
overall: 2063380480 mem 800.363 user 70.887 sys 484.380 real
ls: cannot access '*.cram': No such file or directory