This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 2 licenses out of 31 requested for sentieon:klib
Received only 2 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1796.347 CPU sec, 601.796 real sec
[M::process] read 673448 sequences (100000183 bp) in 0.925 CPU sec, 0.927 real sec...
[M::process] read 673310 sequences (100000098 bp) in 1.460 CPU sec, 1.468 real sec...
[M::process] read 673296 sequences (100000228 bp) in 1.350 CPU sec, 1.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285936, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.38, 133.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 43, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927)
[M::mem_pestat] mean and std.dev: (204.38, 257.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2560)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673288 sequences (100000248 bp) in 1.529 CPU sec, 1.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284993, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.17, 136.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 172, 731)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949)
[M::mem_pestat] mean and std.dev: (248.42, 245.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2558)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285815, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.78, 137.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673448 reads in 312.721 CPU sec, 9.973 real sec
[M::mem_process_seqs] Processed 673310 reads in 210.075 CPU sec, 6.761 real sec
[M::mem_process_seqs] Processed 673296 reads in 217.329 CPU sec, 7.014 real sec
sorting block 0, #reads = 1079767 mem = 536879855 peak = 583630848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285527, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.95, 136.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (292, 1509, 1509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3943)
[M::mem_pestat] mean and std.dev: (874.70, 672.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5160)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673288 reads in 107.559 CPU sec, 3.562 real sec
[M::process] read 673358 sequences (100000271 bp) in 3.339 CPU sec, 3.746 real sec...
sorting block 1, #reads = 1079875 mem = 536879230 peak = 696283136
[M::process] read 673316 sequences (100000100 bp) in 2.509 CPU sec, 2.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285877, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.48, 134.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 207, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (145.90, 132.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1378)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673388 sequences (100000019 bp) in 2.162 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284941, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.16, 134.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 67, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027)
[M::mem_pestat] mean and std.dev: (351.18, 368.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2695)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673358 reads in 206.347 CPU sec, 6.483 real sec
[M::mem_process_seqs] Processed 673316 reads in 114.594 CPU sec, 3.597 real sec
[M::process] read 673350 sequences (100000238 bp) in 2.693 CPU sec, 2.814 real sec...
sorting block 2, #reads = 1079925 mem = 536879775 peak = 734736384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285114, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.14, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 18, 150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (71.40, 122.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673318 sequences (100000232 bp) in 3.293 CPU sec, 3.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285030, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.14, 135.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673388 reads in 205.639 CPU sec, 6.542 real sec
[M::mem_process_seqs] Processed 673350 reads in 112.608 CPU sec, 3.547 real sec
sorting block 3, #reads = 1079823 mem = 536877865 peak = 734736384
[M::process] read 673300 sequences (100000219 bp) in 2.791 CPU sec, 2.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284647, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.10, 136.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1080173 mem = 536877890 peak = 754233344
[M::process] read 673188 sequences (100000230 bp) in 2.599 CPU sec, 2.849 real sec...
[M::process] read 673176 sequences (100000185 bp) in 1.958 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284887, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.37, 137.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 698, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2145)
[M::mem_pestat] mean and std.dev: (446.50, 364.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2856)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673318 reads in 204.079 CPU sec, 6.500 real sec
[M::mem_process_seqs] Processed 673300 reads in 113.376 CPU sec, 3.586 real sec
sorting block 5, #reads = 1079646 mem = 536879296 peak = 754233344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284821, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.51, 137.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672604 sequences (100000250 bp) in 3.018 CPU sec, 3.232 real sec...
[M::process] read 672966 sequences (100000172 bp) in 1.530 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284919, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.00, 141.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673188 reads in 206.054 CPU sec, 6.564 real sec
[M::mem_process_seqs] Processed 673176 reads in 115.649 CPU sec, 3.681 real sec
sorting block 6, #reads = 1079618 mem = 536878942 peak = 754233344
[M::process] read 672416 sequences (100000191 bp) in 1.610 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284037, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 210, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (290.27, 154.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672716 sequences (100000094 bp) in 1.360 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284740, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.60, 145.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284365, 16, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 215, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (294.22, 157.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 92, 173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (98.07, 57.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672604 reads in 307.200 CPU sec, 9.886 real sec
[M::mem_process_seqs] Processed 672966 reads in 210.072 CPU sec, 6.805 real sec
[M::mem_process_seqs] Processed 672416 reads in 218.062 CPU sec, 7.084 real sec
sorting block 7, #reads = 1079494 mem = 536878869 peak = 754233344
sorting block 8, #reads = 1079613 mem = 536877913 peak = 781787136
[M::process] read 673216 sequences (100000146 bp) in 2.226 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284583, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.38, 149.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673332 sequences (100000136 bp) in 1.761 CPU sec, 1.784 real sec...
[M::process] read 673226 sequences (100000247 bp) in 1.550 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285334, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.87, 139.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672716 reads in 211.734 CPU sec, 6.736 real sec
[M::mem_process_seqs] Processed 673216 reads in 113.470 CPU sec, 3.601 real sec
sorting block 9, #reads = 1079196 mem = 536881285 peak = 781787136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285789, 15, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.39, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 67, 1345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3987)
[M::mem_pestat] mean and std.dev: (564.73, 906.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5308)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673202 sequences (100000101 bp) in 3.599 CPU sec, 3.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285175, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.87, 139.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 23, 113)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329)
[M::mem_pestat] mean and std.dev: (53.85, 58.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673362 sequences (100000235 bp) in 2.079 CPU sec, 2.076 real sec...
[M::mem_process_seqs] Processed 673332 reads in 209.324 CPU sec, 6.642 real sec
[M::mem_process_seqs] Processed 673226 reads in 115.959 CPU sec, 3.659 real sec
sorting block 10, #reads = 1079428 mem = 536877771 peak = 781787136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285123, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.18, 138.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673484 sequences (100000064 bp) in 3.069 CPU sec, 3.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285024, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.44, 138.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 22, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (189.40, 272.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673406 sequences (100000206 bp) in 2.666 CPU sec, 2.676 real sec...
[M::mem_process_seqs] Processed 673202 reads in 205.929 CPU sec, 6.524 real sec
sorting block 11, #reads = 1080307 mem = 536879473 peak = 781787136
[M::mem_process_seqs] Processed 673362 reads in 115.940 CPU sec, 3.691 real sec
sorting block 12, #reads = 1079519 mem = 536881548 peak = 852869120
[M::process] read 673376 sequences (100000149 bp) in 2.480 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285226, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.82, 134.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 303, 792)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2116)
[M::mem_pestat] mean and std.dev: (457.92, 418.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2778)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673378 sequences (100000139 bp) in 1.530 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285312, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.03, 134.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284882, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.39, 136.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673484 reads in 304.681 CPU sec, 9.763 real sec
[M::mem_process_seqs] Processed 673406 reads in 211.609 CPU sec, 6.801 real sec
[M::mem_process_seqs] Processed 673376 reads in 219.128 CPU sec, 7.071 real sec
sorting block 13, #reads = 1079694 mem = 536880629 peak = 852869120
[M::process] read 673172 sequences (100000063 bp) in 2.542 CPU sec, 2.791 real sec...
sorting block 14, #reads = 1079981 mem = 536881271 peak = 852869120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284974, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.35, 138.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673082 sequences (100000191 bp) in 1.905 CPU sec, 1.937 real sec...
[M::process] read 673170 sequences (100000229 bp) in 1.650 CPU sec, 1.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285339, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.60, 138.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 86, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226)
[M::mem_pestat] mean and std.dev: (51.62, 40.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 296)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673378 reads in 210.764 CPU sec, 6.704 real sec
[M::mem_process_seqs] Processed 673172 reads in 113.897 CPU sec, 3.592 real sec
sorting block 15, #reads = 1080256 mem = 536879703 peak = 852869120
[M::process] read 672592 sequences (100000100 bp) in 2.406 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284665, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.24, 138.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 65, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256)
[M::mem_pestat] mean and std.dev: (36.38, 33.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 337)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672994 sequences (100000103 bp) in 1.690 CPU sec, 1.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284052, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (289.40, 154.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284871, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.93, 141.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673082 reads in 299.148 CPU sec, 9.577 real sec
[M::mem_process_seqs] Processed 672592 reads in 205.803 CPU sec, 6.608 real sec
[M::mem_process_seqs] Processed 673170 reads in 213.037 CPU sec, 6.860 real sec
sorting block 16, #reads = 1079735 mem = 536878050 peak = 852869120
sorting block 17, #reads = 1079568 mem = 536878151 peak = 852869120
[M::process] read 672596 sequences (100000044 bp) in 2.763 CPU sec, 3.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284514, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.29, 145.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672844 sequences (100000183 bp) in 1.830 CPU sec, 1.858 real sec...
[M::process] read 673060 sequences (100000106 bp) in 1.630 CPU sec, 1.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284724, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (290.04, 155.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 130, 1057)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3047)
[M::mem_pestat] mean and std.dev: (469.93, 538.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4042)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672994 reads in 212.835 CPU sec, 6.781 real sec
[M::mem_process_seqs] Processed 672596 reads in 117.150 CPU sec, 3.695 real sec
sorting block 18, #reads = 1079091 mem = 536879676 peak = 852869120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284509, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (283.34, 150.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673382 sequences (100000043 bp) in 2.508 CPU sec, 2.683 real sec...
[M::process] read 673232 sequences (100000226 bp) in 1.690 CPU sec, 1.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285146, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.13, 143.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 16, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (83.00, 128.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672844 reads in 201.481 CPU sec, 6.421 real sec
[M::mem_process_seqs] Processed 673060 reads in 109.568 CPU sec, 3.491 real sec
sorting block 19, #reads = 1079253 mem = 536880512 peak = 852869120
[M::process] read 673176 sequences (100000090 bp) in 1.646 CPU sec, 1.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285939, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.79, 138.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673134 sequences (100000180 bp) in 1.250 CPU sec, 1.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285919, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.77, 141.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 698, 920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390)
[M::mem_pestat] mean and std.dev: (572.57, 348.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3125)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285987, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.70, 141.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673382 reads in 284.631 CPU sec, 9.132 real sec
[M::mem_process_seqs] Processed 673232 reads in 195.970 CPU sec, 6.322 real sec
sorting block 20, #reads = 1079214 mem = 536877991 peak = 852869120
[M::mem_process_seqs] Processed 673176 reads in 203.733 CPU sec, 6.591 real sec
sorting block 21, #reads = 1080401 mem = 536881980 peak = 852869120
[M::process] read 673362 sequences (100000182 bp) in 2.702 CPU sec, 2.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285017, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.26, 142.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673416 sequences (100000212 bp) in 1.888 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285774, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.41, 137.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673356 sequences (100000139 bp) in 1.620 CPU sec, 1.624 real sec...
[M::mem_process_seqs] Processed 673134 reads in 205.841 CPU sec, 6.540 real sec
[M::mem_process_seqs] Processed 673362 reads in 112.366 CPU sec, 3.543 real sec
sorting block 22, #reads = 1079954 mem = 536878363 peak = 852869120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285473, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.77, 135.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673246 sequences (100000299 bp) in 2.677 CPU sec, 2.897 real sec...
[M::process] read 673284 sequences (100000097 bp) in 1.650 CPU sec, 1.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285289, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.17, 137.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 86, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (61.73, 63.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673416 reads in 197.493 CPU sec, 6.290 real sec
sorting block 23, #reads = 1079836 mem = 536880597 peak = 852869120
[M::mem_process_seqs] Processed 673356 reads in 109.604 CPU sec, 3.494 real sec
sorting block 24, #reads = 1080147 mem = 536879667 peak = 852869120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285636, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.39, 138.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673226 sequences (100000164 bp) in 3.168 CPU sec, 3.348 real sec...
[M::process] read 673102 sequences (100000273 bp) in 1.940 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285201, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.58, 140.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673246 reads in 194.907 CPU sec, 6.186 real sec
[M::mem_process_seqs] Processed 673284 reads in 107.678 CPU sec, 3.392 real sec
sorting block 25, #reads = 1080141 mem = 536879109 peak = 852869120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285522, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.15, 141.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673012 sequences (100000287 bp) in 3.355 CPU sec, 3.516 real sec...
[M::process] read 672922 sequences (100000219 bp) in 1.960 CPU sec, 1.965 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284774, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.92, 142.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673226 reads in 196.115 CPU sec, 6.227 real sec
[M::mem_process_seqs] Processed 673102 reads in 109.770 CPU sec, 3.467 real sec
sorting block 26, #reads = 1080090 mem = 536879706 peak = 852869120
[M::process] read 672820 sequences (100000220 bp) in 2.024 CPU sec, 2.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284995, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.12, 145.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672758 sequences (100000252 bp) in 1.570 CPU sec, 1.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285169, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.61, 146.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284383, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.28, 145.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673012 reads in 282.429 CPU sec, 9.046 real sec
[M::mem_process_seqs] Processed 672820 reads in 195.864 CPU sec, 6.301 real sec
[M::mem_process_seqs] Processed 672922 reads in 203.075 CPU sec, 6.551 real sec
sorting block 27, #reads = 1079911 mem = 536878242 peak = 852869120
sorting block 28, #reads = 1079325 mem = 536879776 peak = 852869120
[M::process] read 672932 sequences (100000180 bp) in 2.746 CPU sec, 2.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285252, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.27, 148.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673290 sequences (100000032 bp) in 1.655 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285077, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.30, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673182 sequences (100000044 bp) in 1.610 CPU sec, 1.604 real sec...
[M::mem_process_seqs] Processed 672758 reads in 197.795 CPU sec, 6.302 real sec
[M::mem_process_seqs] Processed 672932 reads in 107.499 CPU sec, 3.389 real sec
sorting block 29, #reads = 1079919 mem = 536879268 peak = 852869120
[M::process] read 673152 sequences (100000084 bp) in 2.271 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286143, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.11, 135.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 15, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338)
[M::mem_pestat] mean and std.dev: (44.08, 121.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1783)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673110 sequences (100000157 bp) in 1.580 CPU sec, 1.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285400, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.18, 138.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285382, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.16, 139.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 11, 46)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 118)
[M::mem_pestat] mean and std.dev: (16.78, 15.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 154)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673290 reads in 279.559 CPU sec, 9.065 real sec
[M::mem_process_seqs] Processed 673182 reads in 191.424 CPU sec, 6.145 real sec
[M::mem_process_seqs] Processed 673152 reads in 198.491 CPU sec, 6.389 real sec
sorting block 30, #reads = 1078914 mem = 536878245 peak = 852869120
sorting block 31, #reads = 1079892 mem = 536878871 peak = 867938304
[M::process] read 673178 sequences (100000178 bp) in 2.603 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285693, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.27, 139.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673462 sequences (100000030 bp) in 1.753 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285398, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.16, 140.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673110 reads in 194.736 CPU sec, 6.183 real sec
[M::process] read 673394 sequences (100000213 bp) in 1.610 CPU sec, 1.612 real sec...
[M::mem_process_seqs] Processed 673178 reads in 110.154 CPU sec, 3.476 real sec
sorting block 32, #reads = 1079997 mem = 536879730 peak = 867938304
[M::process] read 673238 sequences (100000296 bp) in 2.251 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285567, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.58, 134.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673140 sequences (100000236 bp) in 1.570 CPU sec, 1.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285124, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.79, 135.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285406, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.65, 137.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 98, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2115)
[M::mem_pestat] mean and std.dev: (455.64, 562.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2812)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673462 reads in 282.831 CPU sec, 9.049 real sec
sorting block 33, #reads = 1080161 mem = 536880394 peak = 867938304
[M::mem_process_seqs] Processed 673394 reads in 195.297 CPU sec, 6.262 real sec
[M::mem_process_seqs] Processed 673238 reads in 202.488 CPU sec, 6.510 real sec
sorting block 34, #reads = 1079477 mem = 536879247 peak = 917049344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285259, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (268.02, 136.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 14, 27)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 75)
[M::mem_pestat] mean and std.dev: (15.22, 14.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 99)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673140 reads in 99.200 CPU sec, 3.248 real sec
[M::process] read 673164 sequences (100000032 bp) in 3.193 CPU sec, 3.533 real sec...
sorting block 35, #reads = 1080316 mem = 536880833 peak = 917049344
[M::process] read 673052 sequences (100000217 bp) in 2.314 CPU sec, 2.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285411, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.72, 139.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673116 sequences (100000161 bp) in 1.640 CPU sec, 1.632 real sec...
[M::process] read 672814 sequences (100000143 bp) in 1.489 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285349, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.68, 141.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673164 reads in 190.049 CPU sec, 5.999 real sec
[M::mem_process_seqs] Processed 673052 reads in 105.665 CPU sec, 3.336 real sec
sorting block 36, #reads = 1079796 mem = 536878024 peak = 917049344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285425, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.07, 141.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672862 sequences (100000177 bp) in 3.367 CPU sec, 3.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284963, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.77, 143.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 224, 2064)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6092)
[M::mem_pestat] mean and std.dev: (459.08, 723.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8106)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673116 reads in 192.011 CPU sec, 6.123 real sec
[M::mem_process_seqs] Processed 672814 reads in 103.854 CPU sec, 3.292 real sec
[M::process] read 672750 sequences (100000127 bp) in 1.912 CPU sec, 2.004 real sec...
sorting block 37, #reads = 1080065 mem = 536877848 peak = 917049344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285233, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.09, 144.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672544 sequences (100000089 bp) in 2.772 CPU sec, 3.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285239, 16, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.80, 146.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 199, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1941)
[M::mem_pestat] mean and std.dev: (238.27, 267.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2587)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673454 sequences (100000253 bp) in 1.320 CPU sec, 1.315 real sec...
[M::mem_process_seqs] Processed 672862 reads in 168.340 CPU sec, 5.358 real sec
sorting block 38, #reads = 1079722 mem = 536879931 peak = 917049344
[M::mem_process_seqs] Processed 672750 reads in 93.400 CPU sec, 2.956 real sec
sorting block 39, #reads = 1079628 mem = 536878379 peak = 917049344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285397, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673368 sequences (100000090 bp) in 1.770 CPU sec, 1.918 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.16, 148.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 127, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278)
[M::mem_pestat] mean and std.dev: (169.15, 200.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1693)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673258 sequences (100000216 bp) in 1.640 CPU sec, 1.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284950, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.84, 140.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 32, 65)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159)
[M::mem_pestat] mean and std.dev: (57.40, 53.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 270)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672544 reads in 181.825 CPU sec, 5.810 real sec
[M::mem_process_seqs] Processed 673454 reads in 106.007 CPU sec, 3.389 real sec
sorting block 40, #reads = 1079308 mem = 536880175 peak = 917049344
[M::process] read 673348 sequences (100000297 bp) in 1.806 CPU sec, 1.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284288, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.40, 141.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673570 sequences (100000198 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284314, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.79, 139.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284173, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.62, 141.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673368 reads in 285.522 CPU sec, 9.155 real sec
[M::mem_process_seqs] Processed 673258 reads in 194.786 CPU sec, 6.271 real sec
[M::mem_process_seqs] Processed 673348 reads in 201.643 CPU sec, 6.512 real sec
sorting block 41, #reads = 1079591 mem = 536878470 peak = 917049344
sorting block 42, #reads = 1079550 mem = 536880462 peak = 917049344
[M::process] read 673454 sequences (100000207 bp) in 1.981 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284139, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.42, 134.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673312 sequences (100000226 bp) in 1.529 CPU sec, 1.580 real sec...
[M::process] read 673600 sequences (100000179 bp) in 1.140 CPU sec, 1.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284375, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.71, 139.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673570 reads in 193.639 CPU sec, 6.183 real sec
[M::mem_process_seqs] Processed 673454 reads in 105.595 CPU sec, 3.365 real sec
sorting block 43, #reads = 1079586 mem = 536878999 peak = 917049344
[M::process] read 673370 sequences (100000020 bp) in 2.270 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284639, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.61, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 21, 60)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154)
[M::mem_pestat] mean and std.dev: (22.75, 18.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 201)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673344 sequences (100000054 bp) in 1.150 CPU sec, 1.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284844, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.63, 135.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 359, 1012)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2864)
[M::mem_pestat] mean and std.dev: (576.08, 700.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3790)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284289, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.36, 136.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673312 reads in 269.338 CPU sec, 8.624 real sec
[M::mem_process_seqs] Processed 673600 reads in 180.425 CPU sec, 5.806 real sec
[M::mem_process_seqs] Processed 673370 reads in 186.837 CPU sec, 6.032 real sec
sorting block 44, #reads = 1080306 mem = 536881217 peak = 917049344
sorting block 45, #reads = 1079967 mem = 536879946 peak = 917049344
[M::process] read 673374 sequences (100000015 bp) in 1.759 CPU sec, 1.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284921, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.58, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673164 sequences (100000149 bp) in 1.487 CPU sec, 1.579 real sec...
[M::process] read 672838 sequences (100000131 bp) in 1.170 CPU sec, 1.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284439, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.56, 138.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 380, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (288.62, 300.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673344 reads in 181.169 CPU sec, 5.783 real sec
[M::mem_process_seqs] Processed 673374 reads in 98.597 CPU sec, 3.142 real sec
sorting block 46, #reads = 1080210 mem = 536877887 peak = 917049344
[M::process] read 672940 sequences (100000141 bp) in 1.766 CPU sec, 1.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283834, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.39, 144.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672834 sequences (100000225 bp) in 1.160 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284300, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.18, 146.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283602, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (282.79, 151.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673164 reads in 263.313 CPU sec, 8.442 real sec
[M::mem_process_seqs] Processed 672940 reads in 181.928 CPU sec, 5.856 real sec
sorting block 47, #reads = 1080033 mem = 536878254 peak = 917049344
[M::mem_process_seqs] Processed 672838 reads in 188.731 CPU sec, 6.229 real sec
sorting block 48, #reads = 1079698 mem = 536880095 peak = 917049344
[M::process] read 673098 sequences (100000296 bp) in 2.624 CPU sec, 2.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283881, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.36, 153.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673580 sequences (100000187 bp) in 1.579 CPU sec, 1.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284473, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.10, 147.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 24, 65)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191)
[M::mem_pestat] mean and std.dev: (34.80, 33.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 254)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672834 reads in 184.666 CPU sec, 5.868 real sec
[M::process] read 673354 sequences (100000256 bp) in 1.581 CPU sec, 1.594 real sec...
[M::mem_process_seqs] Processed 673098 reads in 99.442 CPU sec, 3.125 real sec
sorting block 49, #reads = 1079058 mem = 536877723 peak = 917049344
[M::process] read 673416 sequences (100000176 bp) in 1.892 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285318, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.84, 135.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673344 sequences (100000242 bp) in 1.160 CPU sec, 1.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284943, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.91, 136.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 345, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (224.75, 123.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285249, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.59, 138.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673580 reads in 254.796 CPU sec, 8.172 real sec
[M::mem_process_seqs] Processed 673354 reads in 175.812 CPU sec, 5.656 real sec
sorting block 50, #reads = 1079332 mem = 536880187 peak = 917049344
[M::mem_process_seqs] Processed 673416 reads in 182.391 CPU sec, 5.886 real sec
sorting block 51, #reads = 1079786 mem = 536878655 peak = 951877632
[M::process] read 673588 sequences (100000201 bp) in 2.054 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285156, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.91, 134.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673610 sequences (100000097 bp) in 1.433 CPU sec, 1.497 real sec...
[M::process] read 673522 sequences (100000137 bp) in 1.190 CPU sec, 1.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285553, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.49, 131.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673344 reads in 177.892 CPU sec, 5.668 real sec
[M::mem_process_seqs] Processed 673588 reads in 96.793 CPU sec, 3.072 real sec
sorting block 52, #reads = 1079614 mem = 536877717 peak = 951877632
sorting block 53, #reads = 1080114 mem = 536881563 peak = 968142848
[M::process] read 673384 sequences (100000190 bp) in 1.928 CPU sec, 2.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285119, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.39, 134.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673432 sequences (100000045 bp) in 1.150 CPU sec, 1.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284804, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.15, 135.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284886, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.49, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673610 reads in 259.457 CPU sec, 8.312 real sec
[M::mem_process_seqs] Processed 673522 reads in 177.654 CPU sec, 5.726 real sec
[M::mem_process_seqs] Processed 673384 reads in 184.194 CPU sec, 5.953 real sec
sorting block 54, #reads = 1080071 mem = 536880100 peak = 968142848
[M::process] read 673368 sequences (100000176 bp) in 1.858 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284611, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.30, 136.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673346 sequences (100000233 bp) in 1.636 CPU sec, 1.702 real sec...
[M::process] read 673044 sequences (100000265 bp) in 1.550 CPU sec, 1.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284424, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.69, 137.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673432 reads in 180.227 CPU sec, 5.729 real sec
[M::mem_process_seqs] Processed 673368 reads in 98.239 CPU sec, 3.106 real sec
sorting block 55, #reads = 1079999 mem = 536878641 peak = 968142848
sorting block 56, #reads = 1079667 mem = 536879399 peak = 968142848
[M::process] read 673044 sequences (100000096 bp) in 1.711 CPU sec, 1.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284608, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.19, 141.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673032 sequences (100000269 bp) in 1.240 CPU sec, 1.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284044, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (277.43, 148.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 86, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (90.00, 78.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283662, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (278.01, 149.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673346 reads in 267.474 CPU sec, 8.580 real sec
[M::mem_process_seqs] Processed 673044 reads in 184.491 CPU sec, 5.944 real sec
[M::mem_process_seqs] Processed 673044 reads in 191.142 CPU sec, 6.177 real sec
sorting block 57, #reads = 1080154 mem = 536879419 peak = 968142848
[M::process] read 673062 sequences (100000207 bp) in 2.178 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284025, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.64, 150.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1079488 mem = 536879136 peak = 968142848
[M::process] read 673408 sequences (100000180 bp) in 1.214 CPU sec, 1.341 real sec...
[M::process] read 673456 sequences (100000283 bp) in 1.150 CPU sec, 1.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284300, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (278.35, 151.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673032 reads in 183.275 CPU sec, 5.845 real sec
[M::mem_process_seqs] Processed 673062 reads in 99.643 CPU sec, 3.168 real sec
sorting block 59, #reads = 1079355 mem = 536882148 peak = 968142848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285256, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.41, 141.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673230 sequences (100000160 bp) in 2.183 CPU sec, 2.308 real sec...
[M::process] read 673242 sequences (100000146 bp) in 1.510 CPU sec, 1.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285193, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.27, 138.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673408 reads in 171.437 CPU sec, 5.454 real sec
[M::mem_process_seqs] Processed 673456 reads in 94.094 CPU sec, 3.001 real sec
sorting block 60, #reads = 1079448 mem = 536880300 peak = 968142848
[M::process] read 673178 sequences (100000103 bp) in 1.478 CPU sec, 1.564 real sec...
[M::process] read 673538 sequences (100000070 bp) in 1.190 CPU sec, 1.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284949, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.30, 138.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 23, 35)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 67)
[M::mem_pestat] mean and std.dev: (21.22, 10.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 83)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673178 reads in 89.861 CPU sec, 2.899 real sec
sorting block 61, #reads = 1079782 mem = 536877512 peak = 968142848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284624, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.76, 139.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 182, 860)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392)
[M::mem_pestat] mean and std.dev: (403.00, 414.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3158)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673468 sequences (100000188 bp) in 1.471 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284643, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.69, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 461, 1728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4836)
[M::mem_pestat] mean and std.dev: (1387.00, 1686.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8132)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673230 reads in 261.303 CPU sec, 8.345 real sec
[M::mem_process_seqs] Processed 673242 reads in 182.306 CPU sec, 5.845 real sec
sorting block 62, #reads = 1079332 mem = 536879507 peak = 968142848
[M::process] read 673472 sequences (100000038 bp) in 1.571 CPU sec, 1.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284962, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.79, 133.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673412 sequences (100000029 bp) in 1.120 CPU sec, 1.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284573, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.33, 132.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285012, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.33, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 69, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (106.00, 104.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673538 reads in 249.079 CPU sec, 7.990 real sec
[M::mem_process_seqs] Processed 673468 reads in 172.789 CPU sec, 5.568 real sec
[M::mem_process_seqs] Processed 673472 reads in 179.164 CPU sec, 5.791 real sec
sorting block 63, #reads = 1079600 mem = 536883267 peak = 968142848
sorting block 64, #reads = 1079436 mem = 536878869 peak = 968142848
[M::process] read 673422 sequences (100000224 bp) in 2.023 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285084, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (265.99, 138.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673330 sequences (100000200 bp) in 1.592 CPU sec, 1.639 real sec...
[M::process] read 673214 sequences (100000284 bp) in 1.360 CPU sec, 1.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284781, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.48, 139.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673412 reads in 176.709 CPU sec, 5.625 real sec
[M::mem_process_seqs] Processed 673422 reads in 97.326 CPU sec, 3.078 real sec
sorting block 65, #reads = 1079763 mem = 536879438 peak = 968142848
[M::process] read 673086 sequences (100000195 bp) in 1.934 CPU sec, 2.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284524, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.19, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673144 sequences (100000221 bp) in 1.160 CPU sec, 1.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284928, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.06, 145.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284307, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.52, 142.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673330 reads in 255.365 CPU sec, 8.192 real sec
[M::mem_process_seqs] Processed 673086 reads in 176.243 CPU sec, 5.685 real sec
[M::mem_process_seqs] Processed 673214 reads in 182.532 CPU sec, 5.903 real sec
sorting block 66, #reads = 1079970 mem = 536879125 peak = 968142848
sorting block 67, #reads = 1079706 mem = 536881439 peak = 968142848
[M::process] read 673206 sequences (100000299 bp) in 2.033 CPU sec, 2.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284575, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.95, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673052 sequences (100000275 bp) in 1.179 CPU sec, 1.199 real sec...
[M::process] read 673542 sequences (100000003 bp) in 1.150 CPU sec, 1.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285073, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.85, 146.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673144 reads in 176.116 CPU sec, 5.629 real sec
[M::mem_process_seqs] Processed 673206 reads in 94.371 CPU sec, 3.006 real sec
sorting block 68, #reads = 1079522 mem = 536878917 peak = 968142848
[M::process] read 673390 sequences (100000112 bp) in 1.958 CPU sec, 2.060 real sec...
[M::process] read 673338 sequences (100000212 bp) in 1.130 CPU sec, 1.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285354, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.82, 139.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673542 reads in 87.318 CPU sec, 2.804 real sec
sorting block 69, #reads = 1079742 mem = 536879148 peak = 968142848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284626, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.67, 149.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673182 sequences (100000115 bp) in 1.097 CPU sec, 1.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285238, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.57, 140.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673052 reads in 253.895 CPU sec, 8.106 real sec
[M::mem_process_seqs] Processed 673390 reads in 176.485 CPU sec, 5.658 real sec
sorting block 70, #reads = 1079664 mem = 536879318 peak = 968142848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285506, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.80, 143.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 102, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (171.70, 207.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673160 sequences (100000078 bp) in 2.053 CPU sec, 2.181 real sec...
[M::process] read 673626 sequences (100000263 bp) in 1.110 CPU sec, 1.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284579, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.22, 143.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 86, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134)
[M::mem_pestat] mean and std.dev: (157.27, 165.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673338 reads in 166.604 CPU sec, 5.321 real sec
[M::mem_process_seqs] Processed 673182 reads in 91.937 CPU sec, 2.944 real sec
sorting block 71, #reads = 1079493 mem = 536880538 peak = 968142848
[M::process] read 673610 sequences (100000105 bp) in 1.611 CPU sec, 1.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285082, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.17, 142.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673470 sequences (100000036 bp) in 1.090 CPU sec, 1.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285722, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.79, 131.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 20, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (127.17, 214.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1362)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285035, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.99, 134.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673160 reads in 245.142 CPU sec, 7.862 real sec
[M::mem_process_seqs] Processed 673626 reads in 167.484 CPU sec, 5.407 real sec
sorting block 72, #reads = 1079695 mem = 536880649 peak = 968142848
[M::mem_process_seqs] Processed 673610 reads in 173.792 CPU sec, 5.626 real sec
sorting block 73, #reads = 1079777 mem = 536879433 peak = 968142848
[M::process] read 673420 sequences (100000026 bp) in 2.062 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285241, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.87, 133.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673294 sequences (100000165 bp) in 1.282 CPU sec, 1.289 real sec...
[M::process] read 673202 sequences (100000055 bp) in 1.110 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284813, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.83, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673470 reads in 170.448 CPU sec, 5.436 real sec
[M::mem_process_seqs] Processed 673420 reads in 94.386 CPU sec, 2.999 real sec
sorting block 74, #reads = 1079527 mem = 536878361 peak = 968142848
[M::process] read 673220 sequences (100000257 bp) in 1.824 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284947, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.70, 140.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673076 sequences (100000160 bp) in 1.100 CPU sec, 1.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284422, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.83, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 17, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170)
[M::mem_pestat] mean and std.dev: (139.00, 210.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1555)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284639, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.80, 143.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673294 reads in 250.835 CPU sec, 8.039 real sec
sorting block 75, #reads = 1079537 mem = 536879700 peak = 968142848
[M::mem_process_seqs] Processed 673220 reads in 172.571 CPU sec, 5.558 real sec
[M::mem_process_seqs] Processed 673202 reads in 178.964 CPU sec, 5.783 real sec
sorting block 76, #reads = 1079899 mem = 536877774 peak = 1041670144
[M::process] read 673116 sequences (100000240 bp) in 1.940 CPU sec, 2.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284449, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.46, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 25, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192)
[M::mem_pestat] mean and std.dev: (157.71, 241.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1588)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673030 sequences (100000222 bp) in 1.175 CPU sec, 1.192 real sec...
[M::process] read 486934 sequences (72333935 bp) in 0.870 CPU sec, 0.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284690, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.82, 148.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673076 reads in 173.523 CPU sec, 5.554 real sec
sorting block 77, #reads = 1079535 mem = 536878758 peak = 1041670144
[M::mem_process_seqs] Processed 673116 reads in 94.117 CPU sec, 3.009 real sec
sorting block 78, #reads = 1079665 mem = 536879463 peak = 1041670144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 205732, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (278.79, 151.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 486934 reads in 61.328 CPU sec, 2.003 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284384, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.98, 149.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673030 reads in 149.689 CPU sec, 5.168 real sec
sorting block 79, #reads = 1079180 mem = 536878801 peak = 1041670144
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 85985658
overall: 69915299840 mem 13830.805 user 426.072 sys 1013.719 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L005\tPU:L005\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T18-D0003255_R1.fastq.gz 0003.T18-D0003255_R2.fastq.gz
[main] Real time: 1013.758 sec; CPU: 14256.890 sec
sorting block 80, #reads = 230083 mem = 114359771 peak = 1041670144
uniq header count 1
merging 81 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3302774939
output reads: 86608111
bam_mem_sort: 81 calls 42.060 user 0.831 sys 43.069 real
bam_write: 81 calls 18.836 user 1.335 sys 20.098 real
execute: 1 calls 17.179 user 0.948 sys 1032.757 real
merge_files: 1 calls 17.152 user 0.925 sys 18.893 real
parse_chunk: 1284 calls 54.810 user 14.652 sys 70.570 real
read_chunk: 3456 calls 29.569 user 21.050 sys 412.657 real
sort_block: 1 calls 22.878 user 20.456 sys 401.131 real
write_chunk: 2170 calls 16.834 user 0.702 sys 17.753 real
overall: 2396721152 mem 769.920 user 51.475 sys 1032.778 real
ls: cannot access '*.cram': No such file or directory