This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2816.174 CPU sec, 111.665 real sec
[M::process] read 677110 sequences (100000081 bp) in 1.159 CPU sec, 1.167 real sec...
[M::process] read 677098 sequences (100000001 bp) in 1.861 CPU sec, 1.863 real sec...
[M::process] read 677222 sequences (100000283 bp) in 1.630 CPU sec, 1.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288447, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.15, 107.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676856 sequences (100000125 bp) in 1.216 CPU sec, 1.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288722, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.71, 106.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288170, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.64, 105.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 49, 980)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2884)
[M::mem_pestat] mean and std.dev: (427.36, 453.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3836)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677110 reads in 325.280 CPU sec, 10.424 real sec
[M::mem_process_seqs] Processed 677098 reads in 195.851 CPU sec, 6.364 real sec
[M::mem_process_seqs] Processed 677222 reads in 204.856 CPU sec, 6.691 real sec
sorting block 0, #reads = 1082836 mem = 536879693 peak = 588673024
[M::process] read 676928 sequences (100000102 bp) in 2.552 CPU sec, 2.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288442, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.91, 107.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676766 sequences (100000092 bp) in 2.619 CPU sec, 2.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288046, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.52, 109.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 871, 871)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2179)
[M::mem_pestat] mean and std.dev: (674.80, 299.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2833)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676856 reads in 210.685 CPU sec, 7.047 real sec
[M::mem_process_seqs] Processed 676928 reads in 124.546 CPU sec, 4.260 real sec
sorting block 1, #reads = 1081967 mem = 536878956 peak = 761688064
[M::process] read 676952 sequences (100000096 bp) in 2.071 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287732, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.70, 110.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1082551 mem = 536878508 peak = 852463616
[M::process] read 676784 sequences (100000005 bp) in 3.034 CPU sec, 3.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288464, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.88, 109.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 433, 2061)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6113)
[M::mem_pestat] mean and std.dev: (992.60, 1245.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8139)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676766 reads in 209.403 CPU sec, 6.766 real sec
[M::mem_process_seqs] Processed 676952 reads in 118.175 CPU sec, 3.800 real sec
[M::process] read 676604 sequences (100000229 bp) in 1.861 CPU sec, 1.941 real sec...
sorting block 3, #reads = 1082486 mem = 536878299 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287661, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.23, 111.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676662 sequences (100000019 bp) in 2.440 CPU sec, 2.738 real sec...
[M::process] read 676386 sequences (100000095 bp) in 1.842 CPU sec, 1.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287675, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.21, 113.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676784 reads in 213.739 CPU sec, 6.899 real sec
[M::mem_process_seqs] Processed 676604 reads in 120.976 CPU sec, 3.874 real sec
sorting block 4, #reads = 1082494 mem = 536876798 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287455, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.34, 113.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676398 sequences (100000019 bp) in 2.900 CPU sec, 3.296 real sec...
[M::process] read 676408 sequences (100000194 bp) in 1.769 CPU sec, 1.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287816, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.93, 116.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676662 reads in 209.857 CPU sec, 6.755 real sec
[M::mem_process_seqs] Processed 676386 reads in 116.245 CPU sec, 3.757 real sec
sorting block 5, #reads = 1081961 mem = 536879339 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288242, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.47, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676926 sequences (100000255 bp) in 2.734 CPU sec, 3.038 real sec...
[M::process] read 676936 sequences (100000033 bp) in 1.608 CPU sec, 1.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287971, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.22, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676398 reads in 209.677 CPU sec, 6.751 real sec
[M::mem_process_seqs] Processed 676408 reads in 116.995 CPU sec, 3.753 real sec
sorting block 6, #reads = 1082013 mem = 536877038 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288239, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.27, 110.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 208, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (134.18, 69.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 7, #reads = 1081553 mem = 536880748 peak = 852463616
[M::process] read 677092 sequences (100000026 bp) in 2.809 CPU sec, 3.164 real sec...
[M::process] read 676870 sequences (100000204 bp) in 1.765 CPU sec, 1.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288200, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.26, 107.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676926 reads in 207.544 CPU sec, 6.669 real sec
[M::mem_process_seqs] Processed 676936 reads in 118.451 CPU sec, 3.779 real sec
sorting block 8, #reads = 1082381 mem = 536878318 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288151, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.41, 107.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676928 sequences (100000278 bp) in 2.804 CPU sec, 3.143 real sec...
[M::process] read 677102 sequences (100000288 bp) in 1.548 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288112, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.91, 110.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677092 reads in 215.325 CPU sec, 6.942 real sec
[M::mem_process_seqs] Processed 676870 reads in 122.672 CPU sec, 3.951 real sec
sorting block 9, #reads = 1082650 mem = 536879202 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288558, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.97, 111.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676840 sequences (100000172 bp) in 2.951 CPU sec, 3.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288226, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.57, 109.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676714 sequences (100000105 bp) in 2.387 CPU sec, 2.392 real sec...
[M::mem_process_seqs] Processed 676928 reads in 212.112 CPU sec, 7.121 real sec
[M::mem_process_seqs] Processed 677102 reads in 123.244 CPU sec, 4.324 real sec
sorting block 10, #reads = 1082549 mem = 536878677 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288410, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.60, 108.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676654 sequences (100000013 bp) in 3.002 CPU sec, 3.299 real sec...
[M::process] read 676584 sequences (100000220 bp) in 1.526 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288126, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.91, 112.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676840 reads in 214.563 CPU sec, 6.929 real sec
sorting block 11, #reads = 1082838 mem = 536876913 peak = 852463616
[M::mem_process_seqs] Processed 676714 reads in 119.372 CPU sec, 3.842 real sec
sorting block 12, #reads = 1082818 mem = 536881324 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287594, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.32, 114.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676432 sequences (100000042 bp) in 2.418 CPU sec, 2.671 real sec...
[M::process] read 676408 sequences (100000162 bp) in 1.617 CPU sec, 1.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287854, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.94, 115.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676654 reads in 214.455 CPU sec, 6.899 real sec
[M::mem_process_seqs] Processed 676584 reads in 118.868 CPU sec, 3.804 real sec
sorting block 13, #reads = 1082257 mem = 536877235 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288093, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.66, 117.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676662 sequences (100000202 bp) in 3.201 CPU sec, 3.659 real sec...
[M::process] read 676754 sequences (100000059 bp) in 1.727 CPU sec, 1.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288045, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.33, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 102, 131)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363)
[M::mem_pestat] mean and std.dev: (63.88, 52.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676432 reads in 210.785 CPU sec, 6.777 real sec
[M::mem_process_seqs] Processed 676408 reads in 119.021 CPU sec, 3.830 real sec
sorting block 14, #reads = 1081841 mem = 536880676 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288257, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.65, 114.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676972 sequences (100000080 bp) in 3.138 CPU sec, 3.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288561, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.22, 112.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676662 reads in 206.540 CPU sec, 6.630 real sec
[M::process] read 677020 sequences (100000293 bp) in 1.783 CPU sec, 1.888 real sec...
[M::mem_process_seqs] Processed 676754 reads in 116.096 CPU sec, 3.702 real sec
sorting block 15, #reads = 1081743 mem = 536880264 peak = 852463616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288599, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.27, 108.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1082435 mem = 536880392 peak = 855588864
[M::process] read 676902 sequences (100000265 bp) in 2.483 CPU sec, 2.756 real sec...
[M::process] read 676912 sequences (100000236 bp) in 1.674 CPU sec, 1.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288906, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.85, 108.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676972 reads in 206.542 CPU sec, 6.646 real sec
[M::mem_process_seqs] Processed 677020 reads in 115.717 CPU sec, 3.710 real sec
sorting block 17, #reads = 1082861 mem = 536878533 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288511, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.14, 111.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677054 sequences (100000157 bp) in 2.392 CPU sec, 2.779 real sec...
[M::process] read 676916 sequences (100000279 bp) in 1.788 CPU sec, 1.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288152, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.24, 111.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 92, 2605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7657)
[M::mem_pestat] mean and std.dev: (801.79, 1141.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10183)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676902 reads in 209.077 CPU sec, 6.740 real sec
[M::mem_process_seqs] Processed 676912 reads in 117.133 CPU sec, 3.763 real sec
sorting block 18, #reads = 1082695 mem = 536881128 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288450, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.53, 107.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676660 sequences (100000036 bp) in 2.519 CPU sec, 2.882 real sec...
[M::process] read 676510 sequences (100000193 bp) in 1.830 CPU sec, 1.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288493, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.90, 111.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677054 reads in 212.864 CPU sec, 6.860 real sec
[M::mem_process_seqs] Processed 676916 reads in 120.444 CPU sec, 3.849 real sec
sorting block 19, #reads = 1082799 mem = 536878580 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288405, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.34, 113.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676512 sequences (100000088 bp) in 2.545 CPU sec, 2.793 real sec...
[M::process] read 676288 sequences (100000004 bp) in 1.772 CPU sec, 1.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288113, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.78, 114.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676660 reads in 211.638 CPU sec, 6.808 real sec
[M::mem_process_seqs] Processed 676510 reads in 116.120 CPU sec, 3.703 real sec
sorting block 20, #reads = 1082398 mem = 536877384 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287807, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.63, 116.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 85, 108)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270)
[M::mem_pestat] mean and std.dev: (57.00, 32.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 351)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 21, #reads = 1082275 mem = 536878655 peak = 855588864
[M::process] read 676208 sequences (100000259 bp) in 2.648 CPU sec, 3.045 real sec...
[M::process] read 676930 sequences (100000282 bp) in 1.699 CPU sec, 1.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287729, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.95, 118.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676512 reads in 212.191 CPU sec, 6.826 real sec
[M::mem_process_seqs] Processed 676288 reads in 118.934 CPU sec, 3.801 real sec
sorting block 22, #reads = 1082224 mem = 536880799 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287673, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.67, 120.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676664 sequences (100000191 bp) in 2.507 CPU sec, 2.747 real sec...
[M::process] read 677004 sequences (100000000 bp) in 1.895 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287984, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.65, 108.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 90, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (120.90, 113.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676208 reads in 208.755 CPU sec, 6.712 real sec
[M::mem_process_seqs] Processed 676930 reads in 117.147 CPU sec, 3.742 real sec
sorting block 23, #reads = 1081499 mem = 536879113 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287848, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.98, 111.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676844 sequences (100000206 bp) in 2.427 CPU sec, 2.692 real sec...
[M::process] read 676680 sequences (100000137 bp) in 1.834 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288827, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.44, 107.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676664 reads in 205.850 CPU sec, 6.627 real sec
[M::mem_process_seqs] Processed 677004 reads in 116.120 CPU sec, 3.708 real sec
sorting block 24, #reads = 1082243 mem = 536878898 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288758, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.69, 109.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676730 sequences (100000046 bp) in 2.445 CPU sec, 2.761 real sec...
[M::process] read 676782 sequences (100000296 bp) in 1.831 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288875, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.44, 109.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676844 reads in 205.586 CPU sec, 6.625 real sec
[M::mem_process_seqs] Processed 676680 reads in 117.422 CPU sec, 3.763 real sec
sorting block 25, #reads = 1082721 mem = 536878091 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288017, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.20, 110.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1082959 mem = 536878863 peak = 855588864
[M::process] read 676688 sequences (100000176 bp) in 2.495 CPU sec, 2.885 real sec...
[M::process] read 676734 sequences (100000255 bp) in 1.805 CPU sec, 1.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288152, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (227.06, 107.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676730 reads in 212.556 CPU sec, 6.859 real sec
[M::mem_process_seqs] Processed 676782 reads in 119.627 CPU sec, 3.814 real sec
sorting block 27, #reads = 1082496 mem = 536878853 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288408, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.61, 110.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676490 sequences (100000007 bp) in 2.631 CPU sec, 2.945 real sec...
[M::process] read 676406 sequences (100000131 bp) in 1.749 CPU sec, 1.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287978, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.49, 112.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676688 reads in 211.391 CPU sec, 6.828 real sec
[M::mem_process_seqs] Processed 676734 reads in 116.986 CPU sec, 3.773 real sec
sorting block 28, #reads = 1082255 mem = 536877629 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288207, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.51, 113.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676268 sequences (100000168 bp) in 2.776 CPU sec, 3.117 real sec...
[M::process] read 676250 sequences (100000018 bp) in 1.686 CPU sec, 1.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288505, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.63, 117.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 28, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181)
[M::mem_pestat] mean and std.dev: (40.44, 37.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 232)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676490 reads in 209.467 CPU sec, 6.755 real sec
[M::mem_process_seqs] Processed 676406 reads in 115.757 CPU sec, 3.739 real sec
sorting block 29, #reads = 1082290 mem = 536876093 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288504, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.13, 118.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677248 sequences (100000087 bp) in 2.534 CPU sec, 2.852 real sec...
[M::process] read 677356 sequences (100000066 bp) in 1.730 CPU sec, 1.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288262, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.08, 118.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 613, 2612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7780)
[M::mem_pestat] mean and std.dev: (1069.00, 1112.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10364)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676268 reads in 205.437 CPU sec, 6.665 real sec
sorting block 30, #reads = 1082426 mem = 536879893 peak = 855588864
[M::process] read 677308 sequences (100000026 bp) in 2.335 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288327, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.44, 106.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677356 reads in 107.390 CPU sec, 3.546 real sec
sorting block 31, #reads = 1082732 mem = 536880039 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288360, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (222.85, 104.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677248 reads in 217.965 CPU sec, 7.945 real sec
[M::process] read 677150 sequences (100000046 bp) in 2.511 CPU sec, 2.791 real sec...
[M::mem_process_seqs] Processed 676250 reads in 420.312 CPU sec, 14.467 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288012, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.54, 108.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 87, 740)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212)
[M::mem_pestat] mean and std.dev: (389.90, 588.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2948)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 32, #reads = 1082250 mem = 536879113 peak = 855588864
[M::process] read 677106 sequences (100000025 bp) in 2.684 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288211, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.52, 109.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677308 reads in 227.004 CPU sec, 7.379 real sec
[M::mem_process_seqs] Processed 677150 reads in 123.025 CPU sec, 3.938 real sec
[M::process] read 676962 sequences (100000217 bp) in 2.223 CPU sec, 2.451 real sec...
sorting block 33, #reads = 1081363 mem = 536880749 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288070, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.91, 108.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676936 sequences (100000027 bp) in 2.238 CPU sec, 2.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288582, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.30, 108.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676852 sequences (100000211 bp) in 1.793 CPU sec, 1.806 real sec...
[M::mem_process_seqs] Processed 677106 reads in 215.468 CPU sec, 6.995 real sec
[M::mem_process_seqs] Processed 676962 reads in 120.647 CPU sec, 3.917 real sec
sorting block 34, #reads = 1079957 mem = 536878745 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287843, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.16, 108.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 92, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (83.62, 88.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676626 sequences (100000219 bp) in 2.339 CPU sec, 2.583 real sec...
[M::process] read 676776 sequences (100000246 bp) in 1.741 CPU sec, 1.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287444, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.95, 109.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676936 reads in 219.638 CPU sec, 7.076 real sec
sorting block 35, #reads = 1080983 mem = 536880207 peak = 855588864
[M::mem_process_seqs] Processed 676852 reads in 122.272 CPU sec, 3.919 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287699, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.77, 110.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1082219 mem = 536881959 peak = 855588864
[M::process] read 676774 sequences (100000122 bp) in 2.511 CPU sec, 2.972 real sec...
[M::process] read 676656 sequences (100000050 bp) in 1.938 CPU sec, 1.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287374, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.40, 111.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 553, 2362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7054)
[M::mem_pestat] mean and std.dev: (776.00, 963.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9400)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676626 reads in 221.150 CPU sec, 7.154 real sec
[M::mem_process_seqs] Processed 676776 reads in 123.975 CPU sec, 3.973 real sec
sorting block 37, #reads = 1082417 mem = 536878927 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287375, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.00, 114.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676960 sequences (100000064 bp) in 2.646 CPU sec, 2.948 real sec...
[M::process] read 677264 sequences (100000115 bp) in 1.981 CPU sec, 1.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287438, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.27, 116.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 251, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (185.45, 173.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676774 reads in 217.559 CPU sec, 7.017 real sec
[M::mem_process_seqs] Processed 676656 reads in 122.583 CPU sec, 3.934 real sec
sorting block 38, #reads = 1082021 mem = 536879324 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287921, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.89, 110.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 13, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 131)
[M::mem_pestat] mean and std.dev: (26.50, 25.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 171)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677204 sequences (100000194 bp) in 2.862 CPU sec, 3.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288442, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.10, 105.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677234 sequences (100000186 bp) in 1.931 CPU sec, 1.932 real sec...
[M::mem_process_seqs] Processed 676960 reads in 210.654 CPU sec, 6.762 real sec
[M::mem_process_seqs] Processed 677264 reads in 118.486 CPU sec, 3.768 real sec
sorting block 39, #reads = 1082066 mem = 536879033 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288461, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.20, 104.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 180, 1974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5904)
[M::mem_pestat] mean and std.dev: (997.64, 1036.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7869)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677056 sequences (100000215 bp) in 2.737 CPU sec, 2.998 real sec...
[M::process] read 677232 sequences (100000279 bp) in 1.941 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288335, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.41, 107.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677204 reads in 207.329 CPU sec, 6.669 real sec
sorting block 40, #reads = 1081364 mem = 536877360 peak = 855588864
[M::mem_process_seqs] Processed 677234 reads in 117.894 CPU sec, 3.754 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288216, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.64, 110.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1080884 mem = 536878901 peak = 855588864
[M::process] read 677056 sequences (100000086 bp) in 2.724 CPU sec, 3.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288858, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.61, 109.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 73, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (49.67, 38.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 331)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677048 sequences (100000070 bp) in 1.942 CPU sec, 1.949 real sec...
[M::mem_process_seqs] Processed 677056 reads in 210.880 CPU sec, 6.823 real sec
[M::mem_process_seqs] Processed 677232 reads in 121.585 CPU sec, 3.921 real sec
sorting block 42, #reads = 1077392 mem = 536877329 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287914, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.65, 105.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 125, 2350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6846)
[M::mem_pestat] mean and std.dev: (759.45, 985.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9094)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677060 sequences (100000090 bp) in 3.104 CPU sec, 3.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287621, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.53, 108.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677056 reads in 213.127 CPU sec, 6.868 real sec
[M::process] read 676772 sequences (100000164 bp) in 1.811 CPU sec, 1.815 real sec...
[M::mem_process_seqs] Processed 677048 reads in 122.162 CPU sec, 3.914 real sec
sorting block 43, #reads = 1080211 mem = 536877830 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287602, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.56, 110.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676798 sequences (100000170 bp) in 2.813 CPU sec, 3.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287687, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.27, 108.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 78, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242)
[M::mem_pestat] mean and std.dev: (58.90, 44.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 316)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676800 sequences (100000055 bp) in 1.911 CPU sec, 1.917 real sec...
[M::mem_process_seqs] Processed 677060 reads in 215.187 CPU sec, 6.950 real sec
[M::mem_process_seqs] Processed 676772 reads in 125.315 CPU sec, 4.000 real sec
sorting block 44, #reads = 1081719 mem = 536877810 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287858, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.58, 112.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1080994 mem = 536877692 peak = 855588864
[M::process] read 677028 sequences (100000024 bp) in 2.966 CPU sec, 3.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288062, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.76, 113.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 37, 66)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188)
[M::mem_pestat] mean and std.dev: (30.73, 27.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 249)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677028 sequences (100000027 bp) in 1.965 CPU sec, 1.971 real sec...
[M::mem_process_seqs] Processed 676798 reads in 214.880 CPU sec, 6.912 real sec
[M::mem_process_seqs] Processed 676800 reads in 120.652 CPU sec, 3.861 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288104, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.30, 111.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1082192 mem = 536878623 peak = 855588864
[M::process] read 677056 sequences (100000070 bp) in 2.840 CPU sec, 3.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287427, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.91, 109.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677032 sequences (100000072 bp) in 2.043 CPU sec, 2.045 real sec...
[M::mem_process_seqs] Processed 677028 reads in 217.349 CPU sec, 6.996 real sec
[M::mem_process_seqs] Processed 677028 reads in 122.619 CPU sec, 3.900 real sec
sorting block 47, #reads = 1079366 mem = 536878677 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287694, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.34, 108.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 632, 793)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197)
[M::mem_pestat] mean and std.dev: (391.11, 320.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2899)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677006 sequences (100000170 bp) in 2.634 CPU sec, 3.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288454, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.61, 107.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676902 sequences (100000175 bp) in 1.977 CPU sec, 2.017 real sec...
[M::mem_process_seqs] Processed 677056 reads in 210.453 CPU sec, 6.815 real sec
[M::mem_process_seqs] Processed 677032 reads in 118.458 CPU sec, 3.829 real sec
sorting block 48, #reads = 1081673 mem = 536878734 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287784, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.63, 110.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677090 sequences (100000157 bp) in 2.911 CPU sec, 3.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288349, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.86, 112.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677110 sequences (100000257 bp) in 2.037 CPU sec, 2.042 real sec...
[M::mem_process_seqs] Processed 677006 reads in 213.447 CPU sec, 6.854 real sec
[M::mem_process_seqs] Processed 676902 reads in 122.299 CPU sec, 3.888 real sec
sorting block 49, #reads = 1081885 mem = 536880588 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287997, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.77, 106.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1077465 mem = 536879138 peak = 855588864
[M::process] read 676988 sequences (100000210 bp) in 3.021 CPU sec, 3.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287729, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.95, 106.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677090 reads in 205.734 CPU sec, 6.642 real sec
[M::mem_process_seqs] Processed 677110 reads in 121.535 CPU sec, 3.924 real sec
[M::process] read 676894 sequences (100000218 bp) in 2.167 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287349, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 51, #reads = 1080555 mem = 536878136 peak = 855588864
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.43, 109.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676890 sequences (100000075 bp) in 2.953 CPU sec, 3.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287807, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.76, 109.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676988 reads in 217.366 CPU sec, 7.023 real sec
[M::process] read 676818 sequences (100000077 bp) in 1.932 CPU sec, 1.965 real sec...
[M::mem_process_seqs] Processed 676894 reads in 120.856 CPU sec, 3.879 real sec
sorting block 52, #reads = 1081971 mem = 536878954 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288013, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.19, 111.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676748 sequences (100000153 bp) in 3.056 CPU sec, 3.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288069, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.52, 111.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677100 sequences (100000218 bp) in 2.038 CPU sec, 2.046 real sec...
[M::mem_process_seqs] Processed 676890 reads in 220.594 CPU sec, 7.090 real sec
[M::mem_process_seqs] Processed 676818 reads in 124.966 CPU sec, 3.980 real sec
sorting block 53, #reads = 1082063 mem = 536877232 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288033, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.13, 113.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 24, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264)
[M::mem_pestat] mean and std.dev: (26.56, 31.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 350)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677010 sequences (100000175 bp) in 3.125 CPU sec, 3.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287754, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.04, 111.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676748 reads in 206.327 CPU sec, 6.658 real sec
[M::mem_process_seqs] Processed 677100 reads in 118.526 CPU sec, 3.791 real sec
[M::process] read 677116 sequences (100000084 bp) in 2.085 CPU sec, 2.137 real sec...
sorting block 54, #reads = 1080481 mem = 536878741 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288133, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.05, 113.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1080859 mem = 536877310 peak = 855588864
[M::process] read 677084 sequences (100000057 bp) in 2.869 CPU sec, 3.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288566, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.81, 108.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677010 reads in 215.061 CPU sec, 6.987 real sec
[M::process] read 676846 sequences (100000287 bp) in 1.800 CPU sec, 1.882 real sec...
[M::mem_process_seqs] Processed 677116 reads in 119.450 CPU sec, 3.866 real sec
sorting block 56, #reads = 1082294 mem = 536877440 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288299, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.64, 108.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676848 sequences (100000259 bp) in 2.948 CPU sec, 3.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287828, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.46, 111.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 38, 46)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 124)
[M::mem_pestat] mean and std.dev: (26.40, 18.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 163)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676936 sequences (100000185 bp) in 2.012 CPU sec, 2.015 real sec...
[M::mem_process_seqs] Processed 677084 reads in 208.616 CPU sec, 6.720 real sec
[M::mem_process_seqs] Processed 676846 reads in 117.607 CPU sec, 3.781 real sec
sorting block 57, #reads = 1082249 mem = 536879790 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287965, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.56, 109.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677034 sequences (100000269 bp) in 2.737 CPU sec, 3.140 real sec...
[M::process] read 676948 sequences (100000188 bp) in 1.921 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288349, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.49, 108.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 169, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (140.78, 150.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1356)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676848 reads in 216.199 CPU sec, 7.011 real sec
[M::mem_process_seqs] Processed 676936 reads in 122.347 CPU sec, 3.952 real sec
sorting block 58, #reads = 1080710 mem = 536881691 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287551, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.55, 108.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676966 sequences (100000209 bp) in 2.872 CPU sec, 3.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287930, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.49, 109.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676816 sequences (100000060 bp) in 2.013 CPU sec, 2.033 real sec...
[M::mem_process_seqs] Processed 677034 reads in 216.601 CPU sec, 6.971 real sec
[M::mem_process_seqs] Processed 676948 reads in 119.665 CPU sec, 3.815 real sec
sorting block 59, #reads = 1082598 mem = 536878630 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287832, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.67, 111.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 174, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (116.62, 71.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 60, #reads = 1082463 mem = 536877587 peak = 855588864
[M::process] read 676644 sequences (100000256 bp) in 2.767 CPU sec, 3.195 real sec...
[M::process] read 314692 sequences (46512740 bp) in 1.215 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287955, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.74, 113.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 473, 1088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3234)
[M::mem_pestat] mean and std.dev: (565.59, 589.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4307)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676966 reads in 210.996 CPU sec, 6.855 real sec
[M::mem_process_seqs] Processed 676816 reads in 118.934 CPU sec, 3.884 real sec
sorting block 61, #reads = 1082620 mem = 536878058 peak = 855588864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287836, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (231.95, 115.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 539, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385)
[M::mem_pestat] mean and std.dev: (321.55, 242.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1808)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 133797, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.65, 116.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676644 reads in 152.263 CPU sec, 5.015 real sec
[M::mem_process_seqs] Processed 314692 reads in 59.579 CPU sec, 1.965 real sec
sorting block 62, #reads = 1082040 mem = 536881676 peak = 855588864
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 68000364
overall: 69301841920 mem 12979.674 user 956.779 sys 483.662 real
sorting block 63, #reads = 369598 mem = 183422043 peak = 855588864
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L003\tPU:L003\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T21-D0003376_R1.fastq.gz 0002.T21-D0003376_R2.fastq.gz
[main] Real time: 483.721 sec; CPU: 13936.486 sec
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2730149829
output reads: 68525168
bam_mem_sort: 64 calls 56.152 user 1.615 sys 58.469 real
bam_write: 64 calls 44.390 user 4.695 sys 79.719 real
execute: 1 calls 28.090 user 3.637 sys 517.755 real
merge_files: 1 calls 28.066 user 3.604 sys 33.701 real
parse_chunk: 1014 calls 49.232 user 14.769 sys 73.216 real
read_chunk: 2730 calls 36.496 user 14.235 sys 376.194 real
sort_block: 1 calls 26.692 user 12.825 sys 360.755 real
write_chunk: 1714 calls 27.546 user 3.293 sys 32.569 real
overall: 2041810944 mem 854.110 user 71.196 sys 517.771 real
ls: cannot access '*.cram': No such file or directory