This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1880.633 CPU sec, 181.213 real sec
[M::process] read 677032 sequences (100000071 bp) in 1.278 CPU sec, 1.285 real sec...
[M::process] read 676900 sequences (100000225 bp) in 1.990 CPU sec, 2.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288215, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.22, 107.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 76, 579)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723)
[M::mem_pestat] mean and std.dev: (290.30, 442.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2295)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676710 sequences (100000106 bp) in 1.974 CPU sec, 1.999 real sec...
[M::process] read 676686 sequences (100000206 bp) in 1.967 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288196, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.90, 111.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677032 reads in 219.411 CPU sec, 6.924 real sec
[M::mem_process_seqs] Processed 676900 reads in 123.386 CPU sec, 3.918 real sec
sorting block 0, #reads = 1081922 mem = 536877021 peak = 616886272
[M::process] read 677008 sequences (100000056 bp) in 2.242 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288387, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.87, 111.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676882 sequences (100000115 bp) in 1.848 CPU sec, 1.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287419, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.13, 110.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 19, 19)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 21)
[M::mem_pestat] mean and std.dev: (18.00, 1.22)
[M::mem_pestat] low and high boundaries for proper pairs: (13, 23)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287933, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.17, 113.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676710 reads in 318.350 CPU sec, 10.273 real sec
[M::mem_process_seqs] Processed 677008 reads in 228.393 CPU sec, 7.400 real sec
[M::mem_process_seqs] Processed 676686 reads in 239.133 CPU sec, 7.772 real sec
sorting block 1, #reads = 1081573 mem = 536880481 peak = 730046464
[M::process] read 676680 sequences (100000052 bp) in 2.333 CPU sec, 2.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288170, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.33, 110.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1081760 mem = 536878005 peak = 978518016
[M::process] read 676708 sequences (100000043 bp) in 1.999 CPU sec, 2.148 real sec...
[M::process] read 676574 sequences (100000021 bp) in 1.883 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287463, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.52, 112.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676882 reads in 230.977 CPU sec, 7.515 real sec
[M::mem_process_seqs] Processed 676680 reads in 133.234 CPU sec, 4.323 real sec
sorting block 3, #reads = 1082566 mem = 536879570 peak = 978518016
[M::process] read 676710 sequences (100000204 bp) in 2.418 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287433, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.68, 112.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 90, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (104.00, 84.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676264 sequences (100000060 bp) in 1.841 CPU sec, 1.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288045, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.79, 114.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287954, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.44, 114.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676708 reads in 327.646 CPU sec, 10.517 real sec
[M::mem_process_seqs] Processed 676710 reads in 231.401 CPU sec, 7.446 real sec
[M::mem_process_seqs] Processed 676574 reads in 242.350 CPU sec, 7.816 real sec
sorting block 4, #reads = 1081284 mem = 536879411 peak = 978518016
[M::process] read 676236 sequences (100000157 bp) in 2.415 CPU sec, 2.673 real sec...
sorting block 5, #reads = 1082062 mem = 536880541 peak = 991944704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287537, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.80, 118.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (599, 973, 5188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14366)
[M::mem_pestat] mean and std.dev: (2021.50, 2098.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18955)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676590 sequences (100000125 bp) in 2.122 CPU sec, 2.164 real sec...
[M::process] read 676960 sequences (100000259 bp) in 1.871 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287378, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.75, 121.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676264 reads in 225.682 CPU sec, 7.288 real sec
[M::mem_process_seqs] Processed 676236 reads in 129.057 CPU sec, 4.142 real sec
sorting block 6, #reads = 1081903 mem = 536878451 peak = 991944704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288069, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.53, 114.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676734 sequences (100000175 bp) in 2.278 CPU sec, 2.546 real sec...
[M::process] read 676514 sequences (100000286 bp) in 1.834 CPU sec, 1.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288351, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.02, 111.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676590 reads in 213.578 CPU sec, 6.880 real sec
[M::mem_process_seqs] Processed 676960 reads in 121.566 CPU sec, 3.895 real sec
sorting block 7, #reads = 1081493 mem = 536881545 peak = 991944704
[M::process] read 676642 sequences (100000055 bp) in 2.142 CPU sec, 2.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288286, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.79, 113.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676804 sequences (100000269 bp) in 1.985 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288022, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.93, 114.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 426, 1298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3822)
[M::mem_pestat] mean and std.dev: (699.64, 853.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5084)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288184, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.40, 116.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 86, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253)
[M::mem_pestat] mean and std.dev: (41.79, 36.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 334)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676734 reads in 319.006 CPU sec, 10.256 real sec
[M::mem_process_seqs] Processed 676514 reads in 225.735 CPU sec, 7.240 real sec
sorting block 8, #reads = 1082105 mem = 536878893 peak = 1025236992
[M::mem_process_seqs] Processed 676642 reads in 236.724 CPU sec, 7.618 real sec
[M::process] read 676786 sequences (100000166 bp) in 2.118 CPU sec, 2.198 real sec...
sorting block 9, #reads = 1081771 mem = 536881548 peak = 1044799488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287956, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.56, 112.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676678 sequences (100000130 bp) in 2.221 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288307, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.24, 113.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676804 reads in 220.491 CPU sec, 7.151 real sec
[M::process] read 676618 sequences (100000146 bp) in 1.911 CPU sec, 1.912 real sec...
[M::mem_process_seqs] Processed 676786 reads in 126.666 CPU sec, 4.027 real sec
sorting block 10, #reads = 1081994 mem = 536879000 peak = 1044799488
[M::process] read 676510 sequences (100000252 bp) in 2.167 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288263, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.66, 115.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 676, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1589)
[M::mem_pestat] mean and std.dev: (438.60, 305.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2036)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676548 sequences (100000109 bp) in 1.729 CPU sec, 1.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288674, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.79, 114.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 40, 65)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 131)
[M::mem_pestat] mean and std.dev: (43.00, 25.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 164)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676678 reads in 219.071 CPU sec, 7.061 real sec
sorting block 11, #reads = 1082331 mem = 536878279 peak = 1055133696
[M::mem_process_seqs] Processed 676618 reads in 124.094 CPU sec, 3.980 real sec
sorting block 12, #reads = 1082316 mem = 536878412 peak = 1056514048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288178, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.16, 116.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676288 sequences (100000126 bp) in 2.095 CPU sec, 2.407 real sec...
[M::process] read 676052 sequences (100000194 bp) in 1.912 CPU sec, 1.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287696, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.08, 117.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676510 reads in 217.699 CPU sec, 7.029 real sec
[M::mem_process_seqs] Processed 676548 reads in 126.263 CPU sec, 4.066 real sec
sorting block 13, #reads = 1082253 mem = 536879162 peak = 1056514048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287855, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.34, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676814 sequences (100000000 bp) in 2.197 CPU sec, 2.686 real sec...
[M::process] read 676616 sequences (100000251 bp) in 1.895 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288078, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (242.97, 122.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676288 reads in 216.630 CPU sec, 7.006 real sec
[M::mem_process_seqs] Processed 676052 reads in 124.326 CPU sec, 3.951 real sec
sorting block 14, #reads = 1082043 mem = 536878171 peak = 1056514048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288418, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.95, 111.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676546 sequences (100000155 bp) in 2.213 CPU sec, 2.518 real sec...
[M::process] read 676360 sequences (100000186 bp) in 1.902 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288650, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.93, 112.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676814 reads in 207.533 CPU sec, 6.672 real sec
[M::mem_process_seqs] Processed 676616 reads in 119.403 CPU sec, 3.790 real sec
sorting block 15, #reads = 1081259 mem = 536886532 peak = 1064341504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288969, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.32, 116.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 2350, 2350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6622)
[M::mem_pestat] mean and std.dev: (1529.38, 1030.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8758)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676436 sequences (100000164 bp) in 2.318 CPU sec, 2.801 real sec...
sorting block 16, #reads = 1081999 mem = 536877307 peak = 1073545216
[M::process] read 676842 sequences (100000173 bp) in 1.819 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288008, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (238.66, 118.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676546 reads in 212.335 CPU sec, 6.925 real sec
[M::process] read 676498 sequences (100000201 bp) in 1.857 CPU sec, 1.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288512, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.55, 115.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 49, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149)
[M::mem_pestat] mean and std.dev: (31.33, 32.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 198)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676436 reads in 115.688 CPU sec, 3.724 real sec
sorting block 17, #reads = 1082320 mem = 536879663 peak = 1073545216
[M::mem_process_seqs] Processed 676360 reads in 279.713 CPU sec, 9.026 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288737, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.48, 111.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676632 sequences (100000108 bp) in 2.500 CPU sec, 2.756 real sec...
[M::process] read 676582 sequences (100000257 bp) in 1.839 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287593, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.39, 114.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 28, 867)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2587)
[M::mem_pestat] mean and std.dev: (242.06, 363.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3447)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676842 reads in 228.138 CPU sec, 7.341 real sec
sorting block 18, #reads = 1082413 mem = 536879059 peak = 1074913280
[M::mem_process_seqs] Processed 676498 reads in 125.593 CPU sec, 4.012 real sec
[M::process] read 676598 sequences (100000163 bp) in 2.086 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288135, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.21, 116.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1082517 mem = 536878212 peak = 1083236352
[M::process] read 676246 sequences (100000213 bp) in 1.820 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286295, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.44, 115.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676632 reads in 214.231 CPU sec, 7.031 real sec
[M::mem_process_seqs] Processed 676582 reads in 125.328 CPU sec, 4.098 real sec
sorting block 20, #reads = 1082104 mem = 536878064 peak = 1083236352
[M::process] read 676086 sequences (100000270 bp) in 2.468 CPU sec, 2.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288203, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.03, 116.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676330 sequences (100000137 bp) in 1.929 CPU sec, 1.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287459, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.52, 119.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 21, 1859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5537)
[M::mem_pestat] mean and std.dev: (569.09, 795.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7376)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287724, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.32, 121.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 100, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761)
[M::mem_pestat] mean and std.dev: (186.64, 204.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2339)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676598 reads in 318.638 CPU sec, 10.252 real sec
[M::mem_process_seqs] Processed 676246 reads in 224.215 CPU sec, 7.209 real sec
[M::mem_process_seqs] Processed 676086 reads in 235.668 CPU sec, 7.612 real sec
sorting block 21, #reads = 1078915 mem = 536878392 peak = 1083236352
[M::process] read 676746 sequences (100000202 bp) in 2.085 CPU sec, 2.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288263, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.93, 117.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 581, 640)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738)
[M::mem_pestat] mean and std.dev: (398.30, 320.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2287)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 22, #reads = 1081626 mem = 536876960 peak = 1097408512
[M::process] read 676588 sequences (100000131 bp) in 2.391 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288791, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.25, 111.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 6, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1833)
[M::mem_pestat] mean and std.dev: (284.71, 482.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2443)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676330 reads in 217.037 CPU sec, 7.162 real sec
[M::mem_process_seqs] Processed 676746 reads in 126.516 CPU sec, 4.127 real sec
[M::process] read 676360 sequences (100000081 bp) in 2.028 CPU sec, 2.407 real sec...
sorting block 23, #reads = 1081912 mem = 536879880 peak = 1097408512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288338, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.97, 113.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676160 sequences (100000134 bp) in 2.304 CPU sec, 2.417 real sec...
[M::process] read 676970 sequences (100000057 bp) in 1.928 CPU sec, 1.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287728, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.44, 114.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 13, 119)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331)
[M::mem_pestat] mean and std.dev: (34.88, 41.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676588 reads in 218.247 CPU sec, 7.023 real sec
[M::mem_process_seqs] Processed 676360 reads in 126.229 CPU sec, 4.054 real sec
sorting block 24, #reads = 1082226 mem = 536880174 peak = 1097408512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288160, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676782 sequences (100000186 bp) in 2.262 CPU sec, 2.401 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (239.40, 119.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (49, 296, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (193.70, 126.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676462 sequences (100000284 bp) in 1.976 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288762, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.09, 109.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676160 reads in 221.747 CPU sec, 7.142 real sec
[M::mem_process_seqs] Processed 676970 reads in 126.526 CPU sec, 4.070 real sec
sorting block 25, #reads = 1081903 mem = 536878554 peak = 1113780224
[M::process] read 676452 sequences (100000112 bp) in 2.118 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288299, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.42, 113.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 91, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264)
[M::mem_pestat] mean and std.dev: (217.50, 223.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1684)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 26, #reads = 1082403 mem = 536880311 peak = 1133232128
[M::process] read 676480 sequences (100000255 bp) in 1.790 CPU sec, 1.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288294, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.05, 115.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676782 reads in 224.793 CPU sec, 7.284 real sec
[M::mem_process_seqs] Processed 676462 reads in 126.617 CPU sec, 4.033 real sec
sorting block 27, #reads = 1083002 mem = 536880603 peak = 1133232128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288183, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.53, 116.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 678, 1405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3955)
[M::mem_pestat] mean and std.dev: (661.40, 553.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5230)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676356 sequences (100000238 bp) in 2.324 CPU sec, 2.446 real sec...
[M::process] read 676216 sequences (100000199 bp) in 1.997 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287648, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.76, 116.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 91, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786)
[M::mem_pestat] mean and std.dev: (154.50, 190.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2351)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676452 reads in 219.557 CPU sec, 7.061 real sec
[M::mem_process_seqs] Processed 676480 reads in 126.523 CPU sec, 4.048 real sec
sorting block 28, #reads = 1082394 mem = 536877435 peak = 1133232128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287940, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.71, 117.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676104 sequences (100000094 bp) in 2.141 CPU sec, 2.482 real sec...
[M::process] read 677044 sequences (100000070 bp) in 1.943 CPU sec, 1.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287934, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.85, 119.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676356 reads in 220.145 CPU sec, 7.115 real sec
[M::mem_process_seqs] Processed 676216 reads in 124.964 CPU sec, 3.967 real sec
sorting block 29, #reads = 1082485 mem = 536879678 peak = 1133232128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288180, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.51, 121.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677106 sequences (100000082 bp) in 2.244 CPU sec, 2.857 real sec...
[M::process] read 676830 sequences (100000244 bp) in 1.909 CPU sec, 1.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288020, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (230.12, 112.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676104 reads in 221.153 CPU sec, 7.184 real sec
sorting block 30, #reads = 1082312 mem = 536876733 peak = 1146286080
[M::mem_process_seqs] Processed 677044 reads in 129.084 CPU sec, 4.129 real sec
sorting block 31, #reads = 1082097 mem = 536881878 peak = 1151623168
[M::process] read 676828 sequences (100000064 bp) in 2.260 CPU sec, 2.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288363, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.77, 111.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 67, 109)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291)
[M::mem_pestat] mean and std.dev: (68.30, 75.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 382)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676858 sequences (100000070 bp) in 1.898 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287447, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.38, 113.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287559, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.53, 113.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677106 reads in 334.111 CPU sec, 10.764 real sec
[M::mem_process_seqs] Processed 676830 reads in 236.000 CPU sec, 7.580 real sec
[M::mem_process_seqs] Processed 676828 reads in 246.948 CPU sec, 7.953 real sec
sorting block 32, #reads = 1082408 mem = 536880249 peak = 1151623168
[M::process] read 677172 sequences (100000047 bp) in 2.405 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287699, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.82, 108.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1082167 mem = 536879018 peak = 1155469312
[M::process] read 676886 sequences (100000271 bp) in 2.376 CPU sec, 2.549 real sec...
[M::process] read 676894 sequences (100000181 bp) in 1.927 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288012, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.85, 107.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 41, 41)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 89)
[M::mem_pestat] mean and std.dev: (24.00, 14.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 113)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676858 reads in 235.509 CPU sec, 7.607 real sec
[M::mem_process_seqs] Processed 677172 reads in 134.606 CPU sec, 4.311 real sec
sorting block 34, #reads = 1082289 mem = 536878906 peak = 1155469312
[M::process] read 676898 sequences (100000244 bp) in 2.376 CPU sec, 2.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287546, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.72, 108.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676670 sequences (100000216 bp) in 1.859 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287285, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.35, 109.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676886 reads in 237.585 CPU sec, 7.709 real sec
[M::mem_process_seqs] Processed 676894 reads in 133.916 CPU sec, 4.312 real sec
sorting block 35, #reads = 1082594 mem = 536877765 peak = 1155469312
[M::process] read 676690 sequences (100000298 bp) in 2.410 CPU sec, 2.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287943, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.98, 108.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676580 sequences (100000104 bp) in 1.994 CPU sec, 2.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287868, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.21, 114.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 100, 2532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7574)
[M::mem_pestat] mean and std.dev: (858.80, 1121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10095)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287906, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.56, 111.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 49, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205)
[M::mem_pestat] mean and std.dev: (157.25, 213.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1598)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676898 reads in 336.061 CPU sec, 10.830 real sec
[M::mem_process_seqs] Processed 676690 reads in 236.650 CPU sec, 7.631 real sec
[M::mem_process_seqs] Processed 676670 reads in 247.498 CPU sec, 8.003 real sec
sorting block 36, #reads = 1082052 mem = 536878120 peak = 1155469312
[M::process] read 676576 sequences (100000236 bp) in 2.584 CPU sec, 2.785 real sec...
sorting block 37, #reads = 1082310 mem = 536878304 peak = 1168748544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287535, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.13, 116.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 21, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (43.62, 63.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677356 sequences (100000118 bp) in 2.221 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287659, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.56, 116.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677138 sequences (100000065 bp) in 1.974 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 676580 reads in 234.189 CPU sec, 7.566 real sec
[M::mem_process_seqs] Processed 676576 reads in 133.688 CPU sec, 4.283 real sec
sorting block 38, #reads = 1082195 mem = 536881000 peak = 1168748544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288157, 24, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.79, 109.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 17, 1172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3496)
[M::mem_pestat] mean and std.dev: (483.75, 764.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4658)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676972 sequences (100000263 bp) in 2.437 CPU sec, 2.602 real sec...
[M::process] read 676670 sequences (100000189 bp) in 1.976 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287917, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.17, 111.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677356 reads in 221.178 CPU sec, 7.113 real sec
[M::mem_process_seqs] Processed 677138 reads in 123.759 CPU sec, 3.979 real sec
sorting block 39, #reads = 1081659 mem = 536877476 peak = 1168748544
[M::process] read 677160 sequences (100000163 bp) in 2.457 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287688, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.66, 113.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 79, 186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548)
[M::mem_pestat] mean and std.dev: (92.55, 97.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 729)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677114 sequences (100000093 bp) in 1.836 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287607, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.25, 115.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676972 reads in 228.315 CPU sec, 7.398 real sec
sorting block 40, #reads = 1082771 mem = 536882075 peak = 1176793088
[M::mem_process_seqs] Processed 676670 reads in 129.644 CPU sec, 4.159 real sec
sorting block 41, #reads = 1082000 mem = 536881650 peak = 1179111424
[M::process] read 677048 sequences (100000120 bp) in 2.104 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288067, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.98, 107.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676704 sequences (100000044 bp) in 1.900 CPU sec, 1.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288392, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.68, 107.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677160 reads in 227.509 CPU sec, 7.358 real sec
[M::process] read 676860 sequences (100000007 bp) in 2.174 CPU sec, 2.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287711, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.27, 108.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677048 reads in 121.839 CPU sec, 3.881 real sec
sorting block 42, #reads = 1082645 mem = 536877384 peak = 1179111424
[M::mem_process_seqs] Processed 677114 reads in 287.435 CPU sec, 9.200 real sec
[M::process] read 676886 sequences (100000204 bp) in 2.050 CPU sec, 2.368 real sec...
sorting block 43, #reads = 1082780 mem = 536877253 peak = 1194156032
[M::process] read 676642 sequences (100000233 bp) in 1.828 CPU sec, 1.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287730, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.19, 111.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676860 reads in 123.536 CPU sec, 3.984 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283586, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.22, 109.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287998, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.17, 113.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 35, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (148.80, 189.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676554 sequences (100000119 bp) in 2.410 CPU sec, 2.416 real sec...
[M::mem_process_seqs] Processed 676704 reads in 361.121 CPU sec, 11.624 real sec
[M::mem_process_seqs] Processed 676886 reads in 145.041 CPU sec, 4.652 real sec
sorting block 44, #reads = 1082242 mem = 536878439 peak = 1194156032
[M::process] read 677052 sequences (100000029 bp) in 2.269 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288028, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.25, 116.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 265, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202)
[M::mem_pestat] mean and std.dev: (160.50, 147.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1593)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 45, #reads = 1077672 mem = 536879588 peak = 1198444544
[M::process] read 676800 sequences (100000070 bp) in 1.862 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287623, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.58, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 36, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (49.62, 51.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676642 reads in 227.541 CPU sec, 7.417 real sec
[M::process] read 676584 sequences (100000288 bp) in 1.826 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288116, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.52, 111.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677052 reads in 114.600 CPU sec, 3.659 real sec
sorting block 46, #reads = 1082581 mem = 536880356 peak = 1198444544
[M::mem_process_seqs] Processed 676554 reads in 285.401 CPU sec, 9.195 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288073, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.85, 114.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 235, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (235.83, 263.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676552 sequences (100000242 bp) in 2.336 CPU sec, 2.625 real sec...
[M::process] read 676850 sequences (100000157 bp) in 1.946 CPU sec, 1.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288023, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.49, 115.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676800 reads in 227.167 CPU sec, 7.314 real sec
sorting block 47, #reads = 1081714 mem = 536878634 peak = 1198624768
[M::mem_process_seqs] Processed 676584 reads in 126.680 CPU sec, 4.060 real sec
sorting block 48, #reads = 1082200 mem = 536880123 peak = 1198624768
[M::process] read 676990 sequences (100000018 bp) in 2.192 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287657, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.79, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677014 sequences (100000062 bp) in 1.830 CPU sec, 1.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288340, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.40, 111.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287973, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.69, 107.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676552 reads in 327.322 CPU sec, 10.584 real sec
[M::mem_process_seqs] Processed 676850 reads in 229.237 CPU sec, 7.383 real sec
[M::mem_process_seqs] Processed 676990 reads in 240.397 CPU sec, 7.765 real sec
sorting block 49, #reads = 1082043 mem = 536878090 peak = 1198624768
[M::process] read 676852 sequences (100000003 bp) in 2.334 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287949, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.00, 109.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1082365 mem = 536880041 peak = 1199194112
[M::process] read 676714 sequences (100000270 bp) in 2.280 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288076, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (227.91, 110.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677014 reads in 216.148 CPU sec, 6.976 real sec
[M::process] read 676710 sequences (100000195 bp) in 1.950 CPU sec, 1.946 real sec...
[M::mem_process_seqs] Processed 676852 reads in 114.296 CPU sec, 3.611 real sec
sorting block 51, #reads = 1081765 mem = 536876516 peak = 1199194112
[M::process] read 676632 sequences (100000184 bp) in 2.421 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287765, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.24, 112.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676494 sequences (100000262 bp) in 1.980 CPU sec, 1.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288008, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.80, 113.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676714 reads in 191.100 CPU sec, 6.054 real sec
[M::mem_process_seqs] Processed 676710 reads in 108.413 CPU sec, 3.434 real sec
sorting block 52, #reads = 1081825 mem = 536880029 peak = 1199194112
[M::process] read 676976 sequences (100000185 bp) in 2.421 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287900, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.11, 115.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676944 sequences (100000135 bp) in 1.990 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287667, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.24, 117.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 45, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177)
[M::mem_pestat] mean and std.dev: (207.80, 250.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1564)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288191, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (230.18, 114.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676632 reads in 289.351 CPU sec, 9.226 real sec
[M::mem_process_seqs] Processed 676494 reads in 204.554 CPU sec, 6.544 real sec
[M::mem_process_seqs] Processed 676976 reads in 216.137 CPU sec, 6.948 real sec
sorting block 53, #reads = 1082115 mem = 536879222 peak = 1199194112
[M::process] read 676796 sequences (100000136 bp) in 2.451 CPU sec, 2.496 real sec...
sorting block 54, #reads = 1081300 mem = 536878553 peak = 1218658304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287571, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.36, 115.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676610 sequences (100000283 bp) in 2.548 CPU sec, 2.607 real sec...
[M::process] read 676572 sequences (100000236 bp) in 1.944 CPU sec, 1.953 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287249, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.38, 116.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (63, 559, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551)
[M::mem_pestat] mean and std.dev: (454.00, 419.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2133)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676944 reads in 233.518 CPU sec, 7.453 real sec
[M::mem_process_seqs] Processed 676796 reads in 132.981 CPU sec, 4.214 real sec
sorting block 55, #reads = 1081832 mem = 536876911 peak = 1218658304
[M::process] read 676844 sequences (100000034 bp) in 2.478 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287612, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.13, 117.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 70, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553)
[M::mem_pestat] mean and std.dev: (172.42, 218.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2055)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676934 sequences (100000130 bp) in 2.016 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287691, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.96, 113.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288155, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.49, 107.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676610 reads in 326.133 CPU sec, 10.447 real sec
[M::mem_process_seqs] Processed 676572 reads in 227.365 CPU sec, 7.274 real sec
sorting block 56, #reads = 1081728 mem = 536881529 peak = 1218658304
[M::mem_process_seqs] Processed 676844 reads in 238.011 CPU sec, 7.636 real sec
[M::process] read 676740 sequences (100000083 bp) in 2.338 CPU sec, 2.366 real sec...
sorting block 57, #reads = 1081921 mem = 536879229 peak = 1219399680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287640, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.39, 111.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676570 sequences (100000147 bp) in 2.558 CPU sec, 2.594 real sec...
[M::process] read 676646 sequences (100000060 bp) in 2.019 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287753, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.48, 112.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676934 reads in 228.278 CPU sec, 7.291 real sec
[M::mem_process_seqs] Processed 676740 reads in 130.000 CPU sec, 4.101 real sec
sorting block 58, #reads = 1082890 mem = 536879651 peak = 1219399680
[M::process] read 676496 sequences (100000005 bp) in 1.923 CPU sec, 1.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287701, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.33, 114.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676500 sequences (100000294 bp) in 1.500 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287462, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.72, 114.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287626, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.61, 116.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 89, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206)
[M::mem_pestat] mean and std.dev: (146.90, 170.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1607)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676570 reads in 286.008 CPU sec, 9.147 real sec
sorting block 59, #reads = 1081972 mem = 536878963 peak = 1219399680
[M::mem_process_seqs] Processed 676646 reads in 197.244 CPU sec, 6.342 real sec
[M::mem_process_seqs] Processed 676496 reads in 206.535 CPU sec, 6.660 real sec
sorting block 60, #reads = 1082038 mem = 536877388 peak = 1219862528
[M::process] read 446396 sequences (65999752 bp) in 2.210 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287680, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.30, 118.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (556, 1475, 1475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3313)
[M::mem_pestat] mean and std.dev: (975.33, 543.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4232)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 189685, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.64, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676500 reads in 163.479 CPU sec, 5.282 real sec
[M::mem_process_seqs] Processed 446396 reads in 73.777 CPU sec, 2.384 real sec
sorting block 61, #reads = 1082060 mem = 536880080 peak = 1222213632
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67438780
overall: 69810425856 mem 13035.876 user 325.049 sys 563.352 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L001\tPU:L001\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T21-D0003376_R1.fastq.gz 0003.T21-D0003376_R2.fastq.gz
[main] Real time: 563.387 sec; CPU: 13360.929 sec
sorting block 62, #reads = 886398 mem = 439789472 peak = 1222332416
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 2648867850
output reads: 67969794
bam_mem_sort: 63 calls 77.921 user 2.152 sys 81.764 real
bam_write: 63 calls 46.470 user 3.399 sys 95.599 real
execute: 1 calls 21.359 user 0.647 sys 587.527 real
merge_files: 1 calls 21.314 user 0.625 sys 23.267 real
parse_chunk: 1006 calls 90.261 user 16.130 sys 125.475 real
read_chunk: 2708 calls 35.060 user 18.255 sys 375.191 real
sort_block: 1 calls 25.401 user 18.006 sys 374.779 real
write_chunk: 1700 calls 20.732 user 0.444 sys 22.330 real
overall: 2479595520 mem 1033.499 user 50.341 sys 587.547 real
ls: cannot access '*.cram': No such file or directory