.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T18-D0003255;L003;0004)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/79/210bb3e1b50feb2c51090489d7589f/.command.log
Size: 124.6 KB
Task: DAQ:SENTIEON_BWAMEM (T18-D0003255;L003;0004)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/79/210bb3e1b50feb2c51090489d7589f/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/94/29a8cbe3c3c4d2b262932cb234c4b5/T18-D0003255/0004.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/94/29a8cbe3c3c4d2b262932cb234c4b5/T18-D0003255/0004.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/79/210bb3e1b50feb2c51090489d7589f/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1987.950 CPU sec, 190.468 real sec
[M::process] read 673416 sequences (100000073 bp) in 0.957 CPU sec, 0.959 real sec...
[M::process] read 673432 sequences (100000055 bp) in 1.466 CPU sec, 1.523 real sec...
[M::process] read 673526 sequences (100000195 bp) in 1.455 CPU sec, 1.525 real sec...
[M::process] read 673506 sequences (100000170 bp) in 1.396 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285913, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (262.10, 130.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673432 reads in 111.255 CPU sec, 3.661 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285774, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.01, 128.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 87, 990)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2880)
[M::mem_pestat] mean and std.dev: (420.75, 651.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3825)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673434 sequences (100000257 bp) in 1.343 CPU sec, 1.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285968, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.60, 129.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673416 reads in 344.206 CPU sec, 11.295 real sec
[M::mem_process_seqs] Processed 673526 reads in 227.913 CPU sec, 7.435 real sec
sorting block 0, #reads = 1080113 mem = 536880718 peak = 603369472
[M::process] read 673326 sequences (100000031 bp) in 1.478 CPU sec, 1.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285610, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.21, 130.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673312 sequences (100000149 bp) in 1.274 CPU sec, 1.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285090, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.35, 132.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 440, 1946)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5516)
[M::mem_pestat] mean and std.dev: (1137.82, 1377.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7301)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284999, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.40, 134.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673506 reads in 324.979 CPU sec, 10.637 real sec
sorting block 1, #reads = 1079904 mem = 536878826 peak = 916983808
[M::mem_process_seqs] Processed 673434 reads in 227.258 CPU sec, 7.466 real sec
[M::mem_process_seqs] Processed 673326 reads in 235.896 CPU sec, 7.772 real sec
[M::process] read 673256 sequences (100000138 bp) in 1.493 CPU sec, 1.569 real sec...
sorting block 2, #reads = 1079817 mem = 536878887 peak = 963383296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284933, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.90, 135.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673352 sequences (100000146 bp) in 1.885 CPU sec, 1.989 real sec...
[M::process] read 673268 sequences (100000028 bp) in 1.405 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285064, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.41, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 33, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360)
[M::mem_pestat] mean and std.dev: (40.17, 40.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673312 reads in 225.534 CPU sec, 7.305 real sec
[M::mem_process_seqs] Processed 673256 reads in 122.577 CPU sec, 3.916 real sec
sorting block 3, #reads = 1079888 mem = 536879669 peak = 963383296
sorting block 4, #reads = 1079911 mem = 536878371 peak = 963383296
[M::process] read 673174 sequences (100000019 bp) in 1.730 CPU sec, 1.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285272, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.07, 134.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673116 sequences (100000247 bp) in 1.302 CPU sec, 1.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284715, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.32, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 10, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (42.11, 54.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284834, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.23, 139.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673352 reads in 331.952 CPU sec, 10.699 real sec
[M::mem_process_seqs] Processed 673268 reads in 231.378 CPU sec, 7.489 real sec
[M::mem_process_seqs] Processed 673174 reads in 239.945 CPU sec, 7.789 real sec
sorting block 5, #reads = 1079933 mem = 536878336 peak = 963383296
[M::process] read 673008 sequences (100000061 bp) in 1.809 CPU sec, 1.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284779, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (272.53, 141.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672796 sequences (100000221 bp) in 1.806 CPU sec, 1.886 real sec...
[M::process] read 672732 sequences (100000171 bp) in 1.267 CPU sec, 1.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284146, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.60, 142.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673116 reads in 233.115 CPU sec, 7.496 real sec
[M::mem_process_seqs] Processed 673008 reads in 129.140 CPU sec, 4.159 real sec
sorting block 6, #reads = 1079722 mem = 536878709 peak = 963383296
[M::process] read 672710 sequences (100000279 bp) in 1.549 CPU sec, 1.781 real sec...
sorting block 7, #reads = 1079894 mem = 536877837 peak = 983752704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284731, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.13, 144.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672926 sequences (100000195 bp) in 1.326 CPU sec, 1.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284661, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.36, 146.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284946, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (282.68, 148.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 86, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (129.69, 127.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1397)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672796 reads in 330.343 CPU sec, 10.705 real sec
[M::mem_process_seqs] Processed 672732 reads in 228.771 CPU sec, 7.438 real sec
[M::mem_process_seqs] Processed 672710 reads in 237.679 CPU sec, 7.751 real sec
sorting block 8, #reads = 1079339 mem = 536878260 peak = 983752704
[M::process] read 673496 sequences (100000230 bp) in 2.063 CPU sec, 2.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285434, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.06, 143.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 359, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482)
[M::mem_pestat] mean and std.dev: (276.67, 227.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1951)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 9, #reads = 1079244 mem = 536877613 peak = 983752704
[M::process] read 673352 sequences (100000227 bp) in 1.699 CPU sec, 1.807 real sec...
[M::process] read 673492 sequences (100000212 bp) in 1.353 CPU sec, 1.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285642, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.70, 134.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672926 reads in 216.884 CPU sec, 6.971 real sec
[M::mem_process_seqs] Processed 673496 reads in 119.655 CPU sec, 3.868 real sec
sorting block 10, #reads = 1079917 mem = 536880401 peak = 983752704
[M::process] read 673376 sequences (100000264 bp) in 1.904 CPU sec, 2.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285762, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.73, 134.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673328 sequences (100000255 bp) in 1.409 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285587, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.99, 133.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285737, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (261.69, 132.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673352 reads in 323.309 CPU sec, 10.448 real sec
[M::mem_process_seqs] Processed 673376 reads in 225.179 CPU sec, 7.278 real sec
[M::mem_process_seqs] Processed 673492 reads in 233.360 CPU sec, 7.560 real sec
sorting block 11, #reads = 1080139 mem = 536881507 peak = 983752704
[M::process] read 673198 sequences (100000224 bp) in 2.129 CPU sec, 2.347 real sec...
sorting block 12, #reads = 1080334 mem = 536877523 peak = 983752704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285424, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.13, 135.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673282 sequences (100000264 bp) in 1.897 CPU sec, 2.005 real sec...
[M::process] read 673446 sequences (100000203 bp) in 1.609 CPU sec, 1.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285176, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.42, 137.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673328 reads in 224.278 CPU sec, 7.198 real sec
[M::mem_process_seqs] Processed 673198 reads in 125.378 CPU sec, 3.995 real sec
sorting block 13, #reads = 1080405 mem = 536880612 peak = 983752704
[M::process] read 673434 sequences (100000100 bp) in 1.990 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285282, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.16, 137.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 193, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923)
[M::mem_pestat] mean and std.dev: (298.38, 374.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2557)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673172 sequences (100000168 bp) in 1.360 CPU sec, 1.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284938, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.94, 134.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284771, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.79, 135.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673282 reads in 328.172 CPU sec, 10.584 real sec
[M::mem_process_seqs] Processed 673434 reads in 228.044 CPU sec, 7.351 real sec
[M::mem_process_seqs] Processed 673446 reads in 236.216 CPU sec, 7.643 real sec
sorting block 14, #reads = 1080214 mem = 536879788 peak = 983752704
[M::process] read 673196 sequences (100000229 bp) in 2.193 CPU sec, 2.393 real sec...
sorting block 15, #reads = 1080092 mem = 536879268 peak = 983752704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284850, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.92, 138.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672940 sequences (100000240 bp) in 1.750 CPU sec, 1.816 real sec...
[M::process] read 672918 sequences (100000273 bp) in 1.540 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284815, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.80, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673172 reads in 224.169 CPU sec, 7.218 real sec
[M::mem_process_seqs] Processed 673196 reads in 125.481 CPU sec, 4.043 real sec
sorting block 16, #reads = 1079806 mem = 536877950 peak = 983752704
[M::process] read 672840 sequences (100000117 bp) in 2.062 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284303, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.41, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 31, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272)
[M::mem_pestat] mean and std.dev: (26.70, 26.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 361)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672798 sequences (100000175 bp) in 1.377 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285071, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.53, 142.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (85, 607, 607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651)
[M::mem_pestat] mean and std.dev: (390.90, 271.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2173)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284195, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.65, 146.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672940 reads in 334.793 CPU sec, 10.825 real sec
[M::mem_process_seqs] Processed 672918 reads in 231.060 CPU sec, 7.471 real sec
[M::mem_process_seqs] Processed 672840 reads in 239.604 CPU sec, 7.768 real sec
sorting block 17, #reads = 1079619 mem = 536877418 peak = 1007677440
sorting block 18, #reads = 1079426 mem = 536879838 peak = 1007677440
[M::process] read 672622 sequences (100000289 bp) in 2.132 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284439, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.09, 148.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (52, 92, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (112.38, 95.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673412 sequences (100000205 bp) in 1.871 CPU sec, 2.232 real sec...
[M::process] read 673158 sequences (100000167 bp) in 1.433 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285153, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.34, 149.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 87, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830)
[M::mem_pestat] mean and std.dev: (256.82, 279.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2411)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672798 reads in 225.083 CPU sec, 7.260 real sec
[M::mem_process_seqs] Processed 672622 reads in 124.261 CPU sec, 3.985 real sec
sorting block 19, #reads = 1078929 mem = 536881137 peak = 1007677440
[M::process] read 673298 sequences (100000151 bp) in 1.925 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285704, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.21, 134.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673470 sequences (100000284 bp) in 1.459 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285792, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.53, 135.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 39, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (81.50, 117.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1388)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285396, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.99, 137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673412 reads in 311.998 CPU sec, 10.068 real sec
[M::mem_process_seqs] Processed 673298 reads in 219.789 CPU sec, 7.068 real sec
[M::mem_process_seqs] Processed 673158 reads in 228.611 CPU sec, 7.380 real sec
sorting block 20, #reads = 1079241 mem = 536879816 peak = 1007677440
sorting block 21, #reads = 1079940 mem = 536877162 peak = 1007677440
[M::process] read 673332 sequences (100000249 bp) in 2.275 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285897, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.29, 133.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673278 sequences (100000042 bp) in 1.759 CPU sec, 1.951 real sec...
[M::process] read 673266 sequences (100000160 bp) in 1.485 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286071, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.83, 134.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 233, 7783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22883)
[M::mem_pestat] mean and std.dev: (2329.18, 3354.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 30433)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673470 reads in 216.211 CPU sec, 7.024 real sec
[M::mem_process_seqs] Processed 673332 reads in 120.724 CPU sec, 3.896 real sec
sorting block 22, #reads = 1079406 mem = 536878249 peak = 1013833728
[M::process] read 673294 sequences (100000131 bp) in 2.427 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285344, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.87, 137.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673528 sequences (100000169 bp) in 1.509 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284963, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.85, 137.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285384, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.75, 137.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 724, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038)
[M::mem_pestat] mean and std.dev: (507.67, 393.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2695)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673278 reads in 322.578 CPU sec, 10.369 real sec
[M::mem_process_seqs] Processed 673266 reads in 223.338 CPU sec, 7.202 real sec
sorting block 23, #reads = 1079987 mem = 536879378 peak = 1013833728
[M::mem_process_seqs] Processed 673294 reads in 231.385 CPU sec, 7.494 real sec
sorting block 24, #reads = 1080186 mem = 536878977 peak = 1051537408
[M::process] read 673278 sequences (100000009 bp) in 1.777 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285426, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.62, 134.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 345, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2106)
[M::mem_pestat] mean and std.dev: (246.22, 271.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2797)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673054 sequences (100000288 bp) in 2.081 CPU sec, 2.223 real sec...
[M::process] read 672932 sequences (100000122 bp) in 1.449 CPU sec, 1.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285526, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.18, 138.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 96, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489)
[M::mem_pestat] mean and std.dev: (94.27, 73.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673528 reads in 222.012 CPU sec, 7.156 real sec
[M::mem_process_seqs] Processed 673278 reads in 125.227 CPU sec, 3.995 real sec
sorting block 25, #reads = 1080056 mem = 536878851 peak = 1051537408
[M::process] read 672954 sequences (100000299 bp) in 2.233 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284582, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.90, 139.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672858 sequences (100000246 bp) in 1.427 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285121, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.62, 141.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 18, 92)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272)
[M::mem_pestat] mean and std.dev: (42.18, 41.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 362)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285038, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.04, 142.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673054 reads in 331.428 CPU sec, 10.653 real sec
sorting block 26, #reads = 1080251 mem = 536877542 peak = 1061117952
[M::mem_process_seqs] Processed 672932 reads in 233.068 CPU sec, 7.507 real sec
[M::mem_process_seqs] Processed 672954 reads in 241.467 CPU sec, 7.798 real sec
sorting block 27, #reads = 1079307 mem = 536878586 peak = 1070981120
[M::process] read 672914 sequences (100000125 bp) in 2.267 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284506, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.46, 144.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672788 sequences (100000277 bp) in 1.845 CPU sec, 1.960 real sec...
[M::process] read 673034 sequences (100000045 bp) in 1.361 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284908, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.97, 147.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 178, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (101.60, 71.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672858 reads in 220.392 CPU sec, 7.071 real sec
[M::mem_process_seqs] Processed 672914 reads in 120.393 CPU sec, 3.837 real sec
sorting block 28, #reads = 1079731 mem = 536878617 peak = 1070981120
sorting block 29, #reads = 1079972 mem = 536881486 peak = 1070981120
[M::process] read 673320 sequences (100000156 bp) in 1.870 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284841, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.87, 149.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673280 sequences (100000037 bp) in 1.120 CPU sec, 1.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285937, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.48, 141.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286177, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.80, 133.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672788 reads in 300.919 CPU sec, 9.660 real sec
[M::mem_process_seqs] Processed 673034 reads in 206.660 CPU sec, 6.676 real sec
[M::mem_process_seqs] Processed 673320 reads in 215.225 CPU sec, 7.015 real sec
sorting block 30, #reads = 1079166 mem = 536878251 peak = 1070981120
[M::process] read 673336 sequences (100000189 bp) in 2.359 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286214, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.45, 136.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 75, 1208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3544)
[M::mem_pestat] mean and std.dev: (487.38, 560.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4712)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 31, #reads = 1079868 mem = 536880086 peak = 1070981120
[M::process] read 673272 sequences (100000174 bp) in 2.041 CPU sec, 2.186 real sec...
[M::process] read 673332 sequences (100000003 bp) in 1.576 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285928, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.48, 134.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 75, 1814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5304)
[M::mem_pestat] mean and std.dev: (583.50, 805.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7049)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673280 reads in 210.005 CPU sec, 6.715 real sec
[M::mem_process_seqs] Processed 673336 reads in 119.968 CPU sec, 3.806 real sec
sorting block 32, #reads = 1080586 mem = 536876843 peak = 1070981120
[M::process] read 673268 sequences (100000061 bp) in 2.089 CPU sec, 2.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286040, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.47, 133.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673136 sequences (100000235 bp) in 1.522 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285602, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.70, 135.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 129, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483)
[M::mem_pestat] mean and std.dev: (84.25, 70.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285436, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.13, 135.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 23, 47)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 135)
[M::mem_pestat] mean and std.dev: (20.50, 18.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 179)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673272 reads in 314.899 CPU sec, 10.112 real sec
[M::mem_process_seqs] Processed 673332 reads in 219.473 CPU sec, 7.067 real sec
[M::mem_process_seqs] Processed 673268 reads in 227.236 CPU sec, 7.333 real sec
sorting block 33, #reads = 1079794 mem = 536877983 peak = 1070981120
[M::process] read 673256 sequences (100000000 bp) in 2.465 CPU sec, 2.638 real sec...
sorting block 34, #reads = 1079921 mem = 536880944 peak = 1070981120
[M::process] read 673188 sequences (100000046 bp) in 2.001 CPU sec, 2.105 real sec...
[M::process] read 673212 sequences (100000264 bp) in 1.491 CPU sec, 1.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285397, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.19, 135.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 94, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350)
[M::mem_pestat] mean and std.dev: (55.38, 50.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 460)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673188 reads in 108.161 CPU sec, 3.628 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285562, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.21, 136.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 130, 1841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5497)
[M::mem_pestat] mean and std.dev: (508.00, 718.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7325)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673256 reads in 222.306 CPU sec, 7.598 real sec
sorting block 35, #reads = 1079768 mem = 536880504 peak = 1070981120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285380, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.57, 138.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 618, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481)
[M::mem_pestat] mean and std.dev: (489.75, 286.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1855)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673072 sequences (100000103 bp) in 1.852 CPU sec, 1.966 real sec...
[M::process] read 672990 sequences (100000220 bp) in 1.160 CPU sec, 1.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285330, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.42, 136.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 98, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (153.08, 171.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1391)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673136 reads in 437.020 CPU sec, 14.475 real sec
[M::mem_process_seqs] Processed 673212 reads in 127.007 CPU sec, 4.036 real sec
sorting block 36, #reads = 1079752 mem = 536881459 peak = 1070981120
[M::process] read 672926 sequences (100000194 bp) in 1.743 CPU sec, 1.859 real sec...
[M::process] read 672824 sequences (100000112 bp) in 1.040 CPU sec, 1.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285375, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.93, 138.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285161, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.53, 139.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285196, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.78, 141.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (42, 85, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306)
[M::mem_pestat] mean and std.dev: (75.54, 40.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 394)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673072 reads in 299.255 CPU sec, 9.603 real sec
[M::mem_process_seqs] Processed 672990 reads in 206.894 CPU sec, 6.689 real sec
[M::mem_process_seqs] Processed 672926 reads in 215.233 CPU sec, 6.980 real sec
sorting block 37, #reads = 1079837 mem = 536878608 peak = 1070981120
sorting block 38, #reads = 1079612 mem = 536877372 peak = 1070981120
[M::process] read 672822 sequences (100000180 bp) in 2.364 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284978, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.97, 142.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 46, 188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (57.88, 61.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672786 sequences (100000191 bp) in 1.637 CPU sec, 1.686 real sec...
[M::process] read 673688 sequences (100000168 bp) in 1.500 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285118, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.67, 145.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 30, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245)
[M::mem_pestat] mean and std.dev: (224.14, 387.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1773)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672824 reads in 212.130 CPU sec, 6.773 real sec
[M::mem_process_seqs] Processed 672822 reads in 115.418 CPU sec, 3.659 real sec
sorting block 39, #reads = 1079849 mem = 536882243 peak = 1070981120
[M::process] read 673704 sequences (100000060 bp) in 2.188 CPU sec, 2.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285327, 19, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.38, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 194, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (182.94, 197.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673690 sequences (100000255 bp) in 1.150 CPU sec, 1.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285413, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.58, 137.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 186, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326)
[M::mem_pestat] mean and std.dev: (223.88, 248.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1761)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285264, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (261.81, 135.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672786 reads in 293.871 CPU sec, 9.427 real sec
[M::mem_process_seqs] Processed 673688 reads in 203.935 CPU sec, 6.567 real sec
sorting block 40, #reads = 1079610 mem = 536878160 peak = 1070981120
[M::mem_process_seqs] Processed 673704 reads in 212.256 CPU sec, 6.852 real sec
sorting block 41, #reads = 1079589 mem = 536879316 peak = 1070981120
[M::process] read 673516 sequences (100000235 bp) in 1.995 CPU sec, 2.144 real sec...
[M::process] read 673578 sequences (100000160 bp) in 1.503 CPU sec, 1.546 real sec...
[M::process] read 673556 sequences (100000072 bp) in 1.130 CPU sec, 1.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284951, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.76, 135.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 173, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (192.80, 224.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673516 reads in 111.076 CPU sec, 3.540 real sec
sorting block 42, #reads = 1079345 mem = 536878809 peak = 1070981120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285239, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.19, 134.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 204, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (122.38, 77.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673454 sequences (100000253 bp) in 1.214 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284785, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.69, 138.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673690 reads in 312.980 CPU sec, 9.999 real sec
[M::mem_process_seqs] Processed 673578 reads in 117.734 CPU sec, 3.778 real sec
sorting block 43, #reads = 1080277 mem = 536877591 peak = 1070981120
[M::process] read 673320 sequences (100000086 bp) in 1.780 CPU sec, 1.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284956, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.30, 136.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 88, 792)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326)
[M::mem_pestat] mean and std.dev: (210.64, 302.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3093)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673340 sequences (100000137 bp) in 1.090 CPU sec, 1.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285069, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.10, 134.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 102, 8551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25565)
[M::mem_pestat] mean and std.dev: (2170.92, 3505.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34072)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284760, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.78, 136.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 86, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (189.10, 248.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1391)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673556 reads in 284.622 CPU sec, 9.130 real sec
[M::mem_process_seqs] Processed 673320 reads in 203.048 CPU sec, 6.549 real sec
[M::mem_process_seqs] Processed 673454 reads in 211.397 CPU sec, 6.836 real sec
sorting block 44, #reads = 1077465 mem = 536878845 peak = 1070981120
sorting block 45, #reads = 1078370 mem = 536880861 peak = 1070981120
[M::process] read 673294 sequences (100000240 bp) in 2.085 CPU sec, 2.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285269, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.35, 132.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673258 sequences (100000234 bp) in 1.695 CPU sec, 1.739 real sec...
[M::process] read 673342 sequences (100000225 bp) in 1.330 CPU sec, 1.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283834, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.40, 138.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673340 reads in 218.651 CPU sec, 6.980 real sec
[M::mem_process_seqs] Processed 673294 reads in 121.418 CPU sec, 3.873 real sec
sorting block 46, #reads = 1077668 mem = 536880030 peak = 1070981120
[M::process] read 673174 sequences (100000122 bp) in 2.248 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283727, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.99, 139.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673158 sequences (100000248 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283724, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.41, 141.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 122, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3324)
[M::mem_pestat] mean and std.dev: (367.07, 479.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4425)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673258 reads in 222.599 CPU sec, 7.115 real sec
sorting block 47, #reads = 1079291 mem = 536877194 peak = 1070981120
[M::process] read 673016 sequences (100000147 bp) in 1.081 CPU sec, 1.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284004, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.03, 141.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673174 reads in 112.649 CPU sec, 3.643 real sec
[M::mem_process_seqs] Processed 673342 reads in 249.948 CPU sec, 8.068 real sec
sorting block 48, #reads = 1079692 mem = 536876741 peak = 1070981120
[M::process] read 672934 sequences (100000016 bp) in 1.485 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284185, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.08, 145.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673612 sequences (100000149 bp) in 1.100 CPU sec, 1.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283573, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (278.02, 149.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (32, 81, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (184.44, 207.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283940, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.71, 147.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 28, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (135.29, 245.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673158 reads in 306.399 CPU sec, 9.823 real sec
[M::mem_process_seqs] Processed 672934 reads in 205.022 CPU sec, 6.626 real sec
sorting block 49, #reads = 1079727 mem = 536880776 peak = 1071230976
[M::mem_process_seqs] Processed 673016 reads in 212.346 CPU sec, 6.879 real sec
sorting block 50, #reads = 1079483 mem = 536881052 peak = 1071230976
[M::process] read 673554 sequences (100000111 bp) in 1.920 CPU sec, 2.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285473, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.79, 136.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 173, 173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419)
[M::mem_pestat] mean and std.dev: (113.88, 75.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673736 sequences (100000131 bp) in 1.338 CPU sec, 1.394 real sec...
[M::process] read 673576 sequences (100000163 bp) in 1.060 CPU sec, 1.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284995, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.98, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 401, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1661)
[M::mem_pestat] mean and std.dev: (311.10, 259.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2194)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673612 reads in 184.467 CPU sec, 5.900 real sec
[M::mem_process_seqs] Processed 673554 reads in 100.961 CPU sec, 3.221 real sec
sorting block 51, #reads = 1079761 mem = 536879921 peak = 1071230976
[M::process] read 673540 sequences (100000037 bp) in 2.119 CPU sec, 2.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285440, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.40, 130.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (50, 347, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1409)
[M::mem_pestat] mean and std.dev: (286.33, 207.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1862)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673420 sequences (100000038 bp) in 1.470 CPU sec, 1.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285601, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.91, 133.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 52, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (121.75, 131.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285971, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (264.35, 135.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673736 reads in 262.464 CPU sec, 8.396 real sec
[M::mem_process_seqs] Processed 673576 reads in 180.901 CPU sec, 5.812 real sec
sorting block 52, #reads = 1079158 mem = 536877684 peak = 1071230976
[M::mem_process_seqs] Processed 673540 reads in 188.576 CPU sec, 6.077 real sec
sorting block 53, #reads = 1080116 mem = 536876725 peak = 1071230976
[M::process] read 673372 sequences (100000055 bp) in 1.793 CPU sec, 1.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285347, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.23, 137.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673476 sequences (100000275 bp) in 1.794 CPU sec, 1.833 real sec...
[M::process] read 673458 sequences (100000040 bp) in 1.340 CPU sec, 1.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285754, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.16, 137.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673420 reads in 182.399 CPU sec, 5.800 real sec
[M::mem_process_seqs] Processed 673372 reads in 103.273 CPU sec, 3.271 real sec
sorting block 54, #reads = 1075522 mem = 536878182 peak = 1071230976
[M::process] read 673366 sequences (100000191 bp) in 1.936 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285992, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.92, 135.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673360 sequences (100000281 bp) in 1.330 CPU sec, 1.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284399, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.39, 137.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286194, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.66, 133.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 169, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1825)
[M::mem_pestat] mean and std.dev: (273.56, 325.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2407)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673476 reads in 257.263 CPU sec, 8.232 real sec
sorting block 55, #reads = 1074910 mem = 536877191 peak = 1071230976
[M::mem_process_seqs] Processed 673366 reads in 177.006 CPU sec, 5.693 real sec
[M::mem_process_seqs] Processed 673458 reads in 183.479 CPU sec, 5.917 real sec
sorting block 56, #reads = 1079128 mem = 536878606 peak = 1128730624
[M::process] read 673160 sequences (100000004 bp) in 1.832 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284553, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.09, 139.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673208 sequences (100000268 bp) in 1.083 CPU sec, 1.088 real sec...
[M::process] read 673034 sequences (100000022 bp) in 1.200 CPU sec, 1.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284095, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.86, 141.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 91, 3129)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9367)
[M::mem_pestat] mean and std.dev: (1541.82, 1816.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12486)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673360 reads in 177.616 CPU sec, 5.674 real sec
[M::mem_process_seqs] Processed 673160 reads in 103.069 CPU sec, 3.287 real sec
sorting block 57, #reads = 1077811 mem = 536881224 peak = 1132703744
sorting block 58, #reads = 1079109 mem = 536880020 peak = 1132703744
[M::process] read 673088 sequences (100000155 bp) in 1.948 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284378, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.18, 140.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 15, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251)
[M::mem_pestat] mean and std.dev: (51.17, 64.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 333)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673396 sequences (100000201 bp) in 1.130 CPU sec, 1.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284574, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (273.89, 146.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 25, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (135.36, 210.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285066, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.12, 147.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 203, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (186.92, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673208 reads in 267.325 CPU sec, 8.565 real sec
[M::mem_process_seqs] Processed 673034 reads in 184.041 CPU sec, 5.934 real sec
[M::mem_process_seqs] Processed 673088 reads in 191.557 CPU sec, 6.195 real sec
sorting block 59, #reads = 1078107 mem = 536880056 peak = 1132703744
[M::process] read 673474 sequences (100000147 bp) in 2.245 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284862, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (271.90, 144.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673312 sequences (100000051 bp) in 1.162 CPU sec, 1.182 real sec...
[M::process] read 673462 sequences (100000046 bp) in 1.110 CPU sec, 1.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285441, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.76, 137.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673396 reads in 185.912 CPU sec, 5.930 real sec
sorting block 60, #reads = 1077669 mem = 536880094 peak = 1132703744
[M::mem_process_seqs] Processed 673474 reads in 102.840 CPU sec, 3.274 real sec
sorting block 61, #reads = 1077679 mem = 536881109 peak = 1132703744
[M::process] read 673370 sequences (100000268 bp) in 1.668 CPU sec, 1.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284813, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.13, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673336 sequences (100000067 bp) in 1.070 CPU sec, 1.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284976, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.38, 133.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284810, 21, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.56, 132.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 362, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (197.83, 166.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1437)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673312 reads in 247.301 CPU sec, 7.930 real sec
[M::mem_process_seqs] Processed 673462 reads in 166.268 CPU sec, 5.362 real sec
[M::mem_process_seqs] Processed 673370 reads in 173.052 CPU sec, 5.619 real sec
sorting block 62, #reads = 1079925 mem = 536878580 peak = 1132703744
[M::process] read 673340 sequences (100000204 bp) in 1.910 CPU sec, 2.088 real sec...
sorting block 63, #reads = 1079662 mem = 536879688 peak = 1132703744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284626, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.55, 135.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673204 sequences (100000172 bp) in 1.082 CPU sec, 1.094 real sec...
[M::process] read 673336 sequences (100000172 bp) in 1.080 CPU sec, 1.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284650, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.93, 138.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673336 reads in 159.693 CPU sec, 5.086 real sec
[M::mem_process_seqs] Processed 673340 reads in 85.323 CPU sec, 2.712 real sec
sorting block 64, #reads = 1077332 mem = 536882311 peak = 1132703744
[M::process] read 673540 sequences (100000278 bp) in 1.587 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284832, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.70, 139.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 11, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254)
[M::mem_pestat] mean and std.dev: (58.00, 136.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1671)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673518 sequences (100000240 bp) in 1.050 CPU sec, 1.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284715, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.19, 136.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 86, 881)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2577)
[M::mem_pestat] mean and std.dev: (366.61, 485.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3425)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285156, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.85, 134.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (37, 130, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (176.15, 187.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673204 reads in 236.018 CPU sec, 7.564 real sec
[M::mem_process_seqs] Processed 673336 reads in 163.726 CPU sec, 5.283 real sec
[M::mem_process_seqs] Processed 673540 reads in 170.448 CPU sec, 5.518 real sec
sorting block 65, #reads = 1074314 mem = 536877736 peak = 1132703744
[M::process] read 673376 sequences (100000065 bp) in 1.742 CPU sec, 1.920 real sec...
sorting block 66, #reads = 1077757 mem = 536878028 peak = 1132703744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284701, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.43, 137.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673186 sequences (100000050 bp) in 1.183 CPU sec, 1.212 real sec...
[M::process] read 673266 sequences (100000060 bp) in 1.080 CPU sec, 1.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284215, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.71, 140.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673518 reads in 176.890 CPU sec, 5.648 real sec
[M::mem_process_seqs] Processed 673376 reads in 98.568 CPU sec, 3.151 real sec
sorting block 67, #reads = 1079925 mem = 536877276 peak = 1132703744
[M::process] read 673198 sequences (100000094 bp) in 1.641 CPU sec, 1.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283929, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.25, 140.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (55, 78, 1607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4711)
[M::mem_pestat] mean and std.dev: (757.25, 1143.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6263)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673188 sequences (100000051 bp) in 1.090 CPU sec, 1.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283743, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.62, 141.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 53, 2824)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8428)
[M::mem_pestat] mean and std.dev: (893.62, 1287.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11230)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284461, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.05, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673186 reads in 257.994 CPU sec, 8.268 real sec
[M::mem_process_seqs] Processed 673266 reads in 178.799 CPU sec, 5.769 real sec
[M::mem_process_seqs] Processed 673198 reads in 185.781 CPU sec, 6.013 real sec
sorting block 68, #reads = 1079830 mem = 536878714 peak = 1132703744
sorting block 69, #reads = 1079098 mem = 536877205 peak = 1167593472
[M::process] read 673252 sequences (100000147 bp) in 1.821 CPU sec, 1.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285322, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (271.56, 145.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673380 sequences (100000016 bp) in 1.075 CPU sec, 1.082 real sec...
[M::process] read 673404 sequences (100000222 bp) in 1.100 CPU sec, 1.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285089, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (272.83, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673188 reads in 163.873 CPU sec, 5.223 real sec
[M::mem_process_seqs] Processed 673252 reads in 89.248 CPU sec, 2.847 real sec
sorting block 70, #reads = 1076645 mem = 536882069 peak = 1167593472
[M::process] read 673322 sequences (100000047 bp) in 1.603 CPU sec, 1.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285069, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.83, 140.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673352 sequences (100000007 bp) in 1.090 CPU sec, 1.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285148, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.58, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285162, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.21, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 23, 31)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 87)
[M::mem_pestat] mean and std.dev: (12.56, 11.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 115)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673380 reads in 244.151 CPU sec, 7.824 real sec
[M::mem_process_seqs] Processed 673404 reads in 170.333 CPU sec, 5.494 real sec
[M::mem_process_seqs] Processed 673322 reads in 177.209 CPU sec, 5.734 real sec
sorting block 71, #reads = 1078992 mem = 536877627 peak = 1167593472
sorting block 72, #reads = 1079687 mem = 536877781 peak = 1176002560
[M::process] read 673362 sequences (100000065 bp) in 1.704 CPU sec, 1.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285272, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (264.59, 135.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 173, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (204.92, 145.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673260 sequences (100000168 bp) in 1.128 CPU sec, 1.165 real sec...
[M::process] read 673304 sequences (100000001 bp) in 1.100 CPU sec, 1.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285092, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.73, 136.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 10, 10)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22)
[M::mem_pestat] mean and std.dev: (6.38, 3.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 28)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673352 reads in 165.376 CPU sec, 5.271 real sec
[M::mem_process_seqs] Processed 673362 reads in 91.259 CPU sec, 2.906 real sec
sorting block 73, #reads = 1079539 mem = 536880854 peak = 1176002560
[M::process] read 673320 sequences (100000188 bp) in 1.695 CPU sec, 1.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284790, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.86, 139.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673370 sequences (100000044 bp) in 1.080 CPU sec, 1.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285066, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.10, 139.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 220, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (161.90, 120.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284881, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.35, 134.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673260 reads in 244.135 CPU sec, 7.824 real sec
[M::mem_process_seqs] Processed 673304 reads in 169.964 CPU sec, 5.481 real sec
sorting block 74, #reads = 1079535 mem = 536877664 peak = 1176002560
[M::mem_process_seqs] Processed 673320 reads in 177.012 CPU sec, 5.727 real sec
sorting block 75, #reads = 1077523 mem = 536877037 peak = 1188597760
[M::process] read 673402 sequences (100000282 bp) in 1.863 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285001, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.14, 136.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673306 sequences (100000089 bp) in 1.130 CPU sec, 1.152 real sec...
[M::process] read 673208 sequences (100000296 bp) in 1.080 CPU sec, 1.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284599, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.43, 139.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673370 reads in 175.544 CPU sec, 5.604 real sec
[M::mem_process_seqs] Processed 673402 reads in 96.142 CPU sec, 3.068 real sec
sorting block 76, #reads = 1079943 mem = 536877606 peak = 1188597760
[M::process] read 673162 sequences (100000007 bp) in 1.713 CPU sec, 1.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284444, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.41, 140.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673140 sequences (100000262 bp) in 1.070 CPU sec, 1.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284438, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.46, 141.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284109, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.02, 146.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673306 reads in 254.785 CPU sec, 8.167 real sec
sorting block 77, #reads = 1079547 mem = 536879820 peak = 1207459840
[M::mem_process_seqs] Processed 673208 reads in 177.119 CPU sec, 5.713 real sec
[M::mem_process_seqs] Processed 673162 reads in 183.893 CPU sec, 5.951 real sec
sorting block 78, #reads = 1080188 mem = 536877001 peak = 1208078336
[M::process] read 673058 sequences (100000284 bp) in 1.771 CPU sec, 1.938 real sec...
[M::process] read 375330 sequences (55772696 bp) in 0.732 CPU sec, 0.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284088, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.41, 147.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (82, 122, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (103.57, 67.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284314, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.33, 150.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 770, 3210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9456)
[M::mem_pestat] mean and std.dev: (1424.43, 1775.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12579)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 158591, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.16, 149.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673140 reads in 217.801 CPU sec, 7.011 real sec
sorting block 79, #reads = 1079537 mem = 536878442 peak = 1208078336
[M::mem_process_seqs] Processed 673058 reads in 137.585 CPU sec, 4.451 real sec
[M::mem_process_seqs] Processed 375330 reads in 141.484 CPU sec, 4.587 real sec
sorting block 80, #reads = 1079025 mem = 536878124 peak = 1208078336
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 87225464
overall: 69904261120 mem 14937.235 user 521.177 sys 638.772 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L003\tPU:L003\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T18-D0003255_R1.fastq.gz 0004.T18-D0003255_R2.fastq.gz
[main] Real time: 638.817 sec; CPU: 15458.420 sec
sorting block 81, #reads = 434598 mem = 216298778 peak = 1208078336
uniq header count 1
merging 82 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3444489626
output reads: 87854965
bam_mem_sort: 82 calls 39.754 user 0.489 sys 40.851 real
bam_write: 82 calls 37.304 user 2.150 sys 52.106 real
execute: 1 calls 14.008 user 0.495 sys 656.117 real
merge_files: 1 calls 13.979 user 0.476 sys 16.810 real
parse_chunk: 1303 calls 53.013 user 12.025 sys 70.274 real
read_chunk: 3507 calls 23.474 user 20.542 sys 446.795 real
sort_block: 1 calls 18.137 user 20.229 sys 440.034 real
write_chunk: 2202 calls 13.676 user 0.291 sys 15.911 real
overall: 2593681408 mem 727.364 user 45.919 sys 656.138 real
ls: cannot access '*.cram': No such file or directory