File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/0a95191040be259209c95870836ebe/.command.log
Size
90.2 KB
Task
DAQ:SENTIEON_BWAMEM (T21-D0003376;L003;0004)
Attempt

Content

View in new tab 
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/0a95191040be259209c95870836ebe/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bf/010bea11553f8e395b590fdf02ef4b/T21-D0003376/0004.T21-D0003376_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bf/010bea11553f8e395b590fdf02ef4b/T21-D0003376/0004.T21-D0003376_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/0a95191040be259209c95870836ebe/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2760.179 CPU sec, 111.163 real sec
[M::process] read 677082 sequences (100000274 bp) in 1.300 CPU sec, 1.310 real sec...
[M::process] read 677092 sequences (100000105 bp) in 2.000 CPU sec, 2.068 real sec...
[M::process] read 677132 sequences (100000188 bp) in 1.848 CPU sec, 1.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287975, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.19, 107.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677066 sequences (100000032 bp) in 1.683 CPU sec, 1.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288659, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (226.09, 105.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (68, 131, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916)
[M::mem_pestat] mean and std.dev: (398.33, 463.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2532)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288592, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.57, 107.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677082 reads in 325.445 CPU sec, 10.597 real sec
[M::mem_process_seqs] Processed 677092 reads in 199.746 CPU sec, 6.572 real sec
[M::mem_process_seqs] Processed 677132 reads in 207.981 CPU sec, 6.873 real sec
sorting block 0, #reads = 1081941 mem = 536881148 peak = 599244800
[M::process] read 676898 sequences (100000103 bp) in 2.925 CPU sec, 3.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288030, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.74, 108.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676856 sequences (100000108 bp) in 1.911 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287866, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.37, 109.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 226, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (244.73, 174.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677066 reads in 209.531 CPU sec, 6.711 real sec
[M::mem_process_seqs] Processed 676898 reads in 118.356 CPU sec, 3.757 real sec
[M::process] read 677012 sequences (100000103 bp) in 1.851 CPU sec, 1.944 real sec...
sorting block 1, #reads = 1082450 mem = 536881644 peak = 759676928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288360, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (229.01, 109.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676770 sequences (100000006 bp) in 2.754 CPU sec, 2.983 real sec...
sorting block 2, #reads = 1082292 mem = 536879415 peak = 874123264
[M::process] read 676628 sequences (100000209 bp) in 1.551 CPU sec, 1.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288147, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.02, 108.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676856 reads in 215.163 CPU sec, 6.914 real sec
[M::mem_process_seqs] Processed 677012 reads in 119.265 CPU sec, 3.827 real sec
sorting block 3, #reads = 1082804 mem = 536877935 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287723, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.51, 111.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676612 sequences (100000196 bp) in 2.521 CPU sec, 2.838 real sec...
[M::process] read 676392 sequences (100000019 bp) in 1.423 CPU sec, 1.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287749, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.38, 113.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676770 reads in 217.833 CPU sec, 7.045 real sec
[M::mem_process_seqs] Processed 676628 reads in 121.722 CPU sec, 3.929 real sec
sorting block 4, #reads = 1082714 mem = 536880676 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288280, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.39, 114.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676190 sequences (100000235 bp) in 2.754 CPU sec, 3.139 real sec...
[M::process] read 676330 sequences (100000191 bp) in 1.507 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287501, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.85, 116.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 26, 141)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (80.92, 103.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 534)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676612 reads in 217.296 CPU sec, 6.997 real sec
[M::mem_process_seqs] Processed 676392 reads in 122.201 CPU sec, 3.910 real sec
sorting block 5, #reads = 1082150 mem = 536878583 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287858, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.42, 118.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676872 sequences (100000015 bp) in 2.722 CPU sec, 3.138 real sec...
[M::process] read 676946 sequences (100000018 bp) in 1.532 CPU sec, 1.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287793, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.85, 118.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676190 reads in 214.542 CPU sec, 6.903 real sec
sorting block 6, #reads = 1082403 mem = 536877947 peak = 874123264
[M::mem_process_seqs] Processed 676330 reads in 119.373 CPU sec, 3.816 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287897, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.49, 110.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1081800 mem = 536876721 peak = 874123264
[M::process] read 676998 sequences (100000166 bp) in 3.043 CPU sec, 3.342 real sec...
[M::process] read 676866 sequences (100000178 bp) in 1.672 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288907, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.33, 107.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676872 reads in 213.751 CPU sec, 6.878 real sec
[M::mem_process_seqs] Processed 676946 reads in 121.164 CPU sec, 3.881 real sec
sorting block 8, #reads = 1082234 mem = 536878308 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288457, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.96, 108.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676894 sequences (100000119 bp) in 3.171 CPU sec, 3.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288380, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.52, 110.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676998 sequences (100000235 bp) in 1.611 CPU sec, 1.617 real sec...
[M::mem_process_seqs] Processed 676998 reads in 216.310 CPU sec, 6.968 real sec
[M::mem_process_seqs] Processed 676866 reads in 122.323 CPU sec, 3.937 real sec
sorting block 9, #reads = 1082656 mem = 536876492 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288564, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.52, 110.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 32, 40)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 106)
[M::mem_pestat] mean and std.dev: (25.57, 21.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 139)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676980 sequences (100000114 bp) in 2.936 CPU sec, 3.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288623, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.63, 110.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676742 sequences (100000251 bp) in 1.578 CPU sec, 1.587 real sec...
[M::mem_process_seqs] Processed 676894 reads in 218.445 CPU sec, 7.043 real sec
[M::mem_process_seqs] Processed 676998 reads in 124.242 CPU sec, 3.986 real sec
sorting block 10, #reads = 1082929 mem = 536878275 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288371, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.56, 108.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676604 sequences (100000107 bp) in 3.025 CPU sec, 3.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288124, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.60, 111.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676566 sequences (100000257 bp) in 1.833 CPU sec, 1.841 real sec...
[M::mem_process_seqs] Processed 676980 reads in 220.796 CPU sec, 7.104 real sec
sorting block 11, #reads = 1082827 mem = 536879826 peak = 874123264
[M::mem_process_seqs] Processed 676742 reads in 126.025 CPU sec, 4.032 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287691, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.41, 113.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 58, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102)
[M::mem_pestat] mean and std.dev: (196.20, 223.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1464)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 12, #reads = 1082572 mem = 536878557 peak = 874123264
[M::process] read 676532 sequences (100000082 bp) in 2.919 CPU sec, 3.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288295, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.94, 115.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676604 reads in 220.327 CPU sec, 7.087 real sec
[M::process] read 676212 sequences (100000252 bp) in 2.324 CPU sec, 2.345 real sec...
[M::mem_process_seqs] Processed 676566 reads in 124.281 CPU sec, 3.963 real sec
sorting block 13, #reads = 1082012 mem = 536879309 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288044, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.27, 117.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676536 sequences (100000135 bp) in 3.405 CPU sec, 3.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287648, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.18, 120.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676532 reads in 215.415 CPU sec, 6.947 real sec
[M::process] read 676616 sequences (100000184 bp) in 1.609 CPU sec, 1.611 real sec...
[M::mem_process_seqs] Processed 676212 reads in 122.311 CPU sec, 3.927 real sec
sorting block 14, #reads = 1082473 mem = 536878635 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288231, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.24, 114.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (73, 438, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2302)
[M::mem_pestat] mean and std.dev: (365.75, 312.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3045)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676904 sequences (100000097 bp) in 3.393 CPU sec, 3.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288760, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.58, 112.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676536 reads in 213.329 CPU sec, 7.172 real sec
[M::mem_process_seqs] Processed 676616 reads in 122.247 CPU sec, 4.234 real sec
[M::process] read 676874 sequences (100000129 bp) in 2.429 CPU sec, 2.538 real sec...
sorting block 15, #reads = 1081807 mem = 536878344 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288646, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.66, 108.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1082004 mem = 536878990 peak = 874123264
[M::process] read 676874 sequences (100000156 bp) in 2.421 CPU sec, 2.738 real sec...
[M::process] read 676902 sequences (100000034 bp) in 1.663 CPU sec, 1.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288980, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.50, 109.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 89, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (159.33, 228.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676904 reads in 210.532 CPU sec, 7.034 real sec
[M::mem_process_seqs] Processed 676874 reads in 117.904 CPU sec, 3.954 real sec
sorting block 17, #reads = 1082623 mem = 536880973 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288806, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.19, 110.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676896 sequences (100000220 bp) in 2.700 CPU sec, 2.951 real sec...
[M::process] read 676694 sequences (100000056 bp) in 1.905 CPU sec, 1.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288016, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.01, 112.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676874 reads in 214.037 CPU sec, 6.871 real sec
[M::mem_process_seqs] Processed 676902 reads in 120.432 CPU sec, 3.841 real sec
sorting block 18, #reads = 1083120 mem = 536879160 peak = 874123264
[M::process] read 676544 sequences (100000201 bp) in 2.642 CPU sec, 2.936 real sec...
[M::process] read 676524 sequences (100000278 bp) in 1.750 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288252, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.74, 112.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 346, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2082)
[M::mem_pestat] mean and std.dev: (395.09, 335.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2761)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676694 reads in 113.180 CPU sec, 3.616 real sec
sorting block 19, #reads = 1082155 mem = 536878508 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288492, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.62, 106.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676382 sequences (100000100 bp) in 1.724 CPU sec, 1.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288222, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.51, 113.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676896 reads in 327.395 CPU sec, 10.517 real sec
[M::mem_process_seqs] Processed 676544 reads in 130.466 CPU sec, 4.239 real sec
sorting block 20, #reads = 1082683 mem = 536878495 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288361, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.97, 114.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676228 sequences (100000081 bp) in 2.812 CPU sec, 3.180 real sec...
[M::process] read 676104 sequences (100000278 bp) in 1.754 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287740, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.50, 116.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676524 reads in 216.818 CPU sec, 6.968 real sec
[M::mem_process_seqs] Processed 676382 reads in 119.893 CPU sec, 3.840 real sec
sorting block 21, #reads = 1082314 mem = 536879924 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287227, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.28, 119.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676914 sequences (100000041 bp) in 2.375 CPU sec, 2.671 real sec...
[M::process] read 676674 sequences (100000262 bp) in 2.033 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288075, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.67, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676228 reads in 217.864 CPU sec, 7.008 real sec
[M::mem_process_seqs] Processed 676104 reads in 119.739 CPU sec, 3.825 real sec
sorting block 22, #reads = 1081773 mem = 536883049 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288210, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.29, 109.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 17, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (151.36, 199.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676978 sequences (100000146 bp) in 2.434 CPU sec, 2.743 real sec...
[M::process] read 676810 sequences (100000020 bp) in 1.712 CPU sec, 1.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288419, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.93, 111.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676914 reads in 212.352 CPU sec, 6.847 real sec
[M::mem_process_seqs] Processed 676674 reads in 119.015 CPU sec, 3.817 real sec
sorting block 23, #reads = 1081899 mem = 536878955 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288311, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.52, 108.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1082394 mem = 536879182 peak = 874123264
[M::process] read 676794 sequences (100000097 bp) in 2.920 CPU sec, 3.337 real sec...
[M::process] read 676746 sequences (100000058 bp) in 1.845 CPU sec, 1.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288221, 16, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.50, 109.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 426, 1962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5744)
[M::mem_pestat] mean and std.dev: (531.21, 683.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7635)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676978 reads in 213.261 CPU sec, 6.864 real sec
[M::mem_process_seqs] Processed 676810 reads in 120.906 CPU sec, 3.877 real sec
sorting block 25, #reads = 1082205 mem = 536879063 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288229, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.24, 109.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676868 sequences (100000133 bp) in 2.750 CPU sec, 2.990 real sec...
[M::process] read 676926 sequences (100000185 bp) in 1.898 CPU sec, 1.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288812, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.72, 110.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676794 reads in 216.013 CPU sec, 6.949 real sec
[M::mem_process_seqs] Processed 676746 reads in 121.615 CPU sec, 3.881 real sec
sorting block 26, #reads = 1081902 mem = 536880038 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288584, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.84, 107.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676688 sequences (100000014 bp) in 2.501 CPU sec, 2.679 real sec...
[M::process] read 676600 sequences (100000271 bp) in 1.671 CPU sec, 1.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288275, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.81, 109.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676868 reads in 216.069 CPU sec, 6.986 real sec
[M::mem_process_seqs] Processed 676926 reads in 121.948 CPU sec, 3.942 real sec
sorting block 27, #reads = 1082610 mem = 536880414 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288386, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.47, 112.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676228 sequences (100000023 bp) in 2.417 CPU sec, 2.702 real sec...
[M::process] read 676242 sequences (100000204 bp) in 1.770 CPU sec, 1.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288281, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.43, 114.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676688 reads in 216.289 CPU sec, 6.962 real sec
sorting block 28, #reads = 1082436 mem = 536878392 peak = 874123264
[M::mem_process_seqs] Processed 676600 reads in 121.209 CPU sec, 3.868 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288145, 15, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.17, 116.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 24, 758)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226)
[M::mem_pestat] mean and std.dev: (124.17, 224.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2960)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 29, #reads = 1082783 mem = 536880189 peak = 874123264
[M::process] read 676240 sequences (100000278 bp) in 2.656 CPU sec, 2.992 real sec...
[M::process] read 677370 sequences (100000125 bp) in 1.644 CPU sec, 1.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287606, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.88, 118.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676228 reads in 215.250 CPU sec, 6.953 real sec
[M::mem_process_seqs] Processed 676242 reads in 121.152 CPU sec, 3.882 real sec
sorting block 30, #reads = 1081879 mem = 536880074 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287795, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.03, 117.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677364 sequences (100000143 bp) in 2.772 CPU sec, 3.062 real sec...
[M::process] read 677322 sequences (100000275 bp) in 1.846 CPU sec, 1.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288197, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (222.74, 104.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676240 reads in 216.052 CPU sec, 6.942 real sec
[M::mem_process_seqs] Processed 677370 reads in 122.451 CPU sec, 3.929 real sec
sorting block 31, #reads = 1081573 mem = 536879974 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287922, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.87, 105.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677236 sequences (100000199 bp) in 2.742 CPU sec, 2.947 real sec...
[M::process] read 677158 sequences (100000262 bp) in 1.952 CPU sec, 1.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288058, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.65, 108.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (421, 421, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 1000)
[M::mem_pestat] mean and std.dev: (455.67, 197.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677364 reads in 225.803 CPU sec, 7.255 real sec
[M::mem_process_seqs] Processed 677322 reads in 128.274 CPU sec, 4.122 real sec
sorting block 32, #reads = 1081829 mem = 536878816 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288388, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.62, 109.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1082007 mem = 536878206 peak = 874123264
[M::process] read 676852 sequences (100000090 bp) in 2.657 CPU sec, 2.992 real sec...
[M::process] read 677038 sequences (100000180 bp) in 1.790 CPU sec, 1.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287869, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.01, 108.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 17, 139)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (28.00, 38.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677236 reads in 218.269 CPU sec, 7.056 real sec
[M::mem_process_seqs] Processed 677158 reads in 121.717 CPU sec, 3.931 real sec
sorting block 34, #reads = 1079566 mem = 536879127 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287749, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.24, 107.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677008 sequences (100000193 bp) in 3.089 CPU sec, 3.454 real sec...
[M::process] read 676892 sequences (100000075 bp) in 1.811 CPU sec, 1.814 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287644, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.73, 108.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676852 reads in 214.959 CPU sec, 6.914 real sec
[M::mem_process_seqs] Processed 677038 reads in 123.325 CPU sec, 3.954 real sec
sorting block 35, #reads = 1080890 mem = 536880530 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287339, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.92, 110.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676902 sequences (100000118 bp) in 2.363 CPU sec, 2.794 real sec...
[M::process] read 676778 sequences (100000178 bp) in 1.757 CPU sec, 1.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287576, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.25, 111.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677008 reads in 218.039 CPU sec, 7.032 real sec
[M::mem_process_seqs] Processed 676892 reads in 122.533 CPU sec, 3.942 real sec
sorting block 36, #reads = 1082417 mem = 536881937 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287669, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.88, 111.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676634 sequences (100000128 bp) in 2.553 CPU sec, 2.851 real sec...
[M::process] read 677026 sequences (100000217 bp) in 1.729 CPU sec, 1.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287426, 12, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.07, 113.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 131, 742)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2170)
[M::mem_pestat] mean and std.dev: (390.50, 481.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2884)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676902 reads in 217.684 CPU sec, 7.033 real sec
[M::mem_process_seqs] Processed 676778 reads in 121.183 CPU sec, 3.913 real sec
sorting block 37, #reads = 1082486 mem = 536879561 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287360, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.11, 114.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1082490 mem = 536878233 peak = 874123264
[M::process] read 677284 sequences (100000248 bp) in 2.379 CPU sec, 2.646 real sec...
[M::process] read 677352 sequences (100000052 bp) in 1.679 CPU sec, 1.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287881, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.85, 110.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 229, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870)
[M::mem_pestat] mean and std.dev: (417.40, 391.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2463)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676634 reads in 212.158 CPU sec, 6.817 real sec
[M::mem_process_seqs] Processed 677026 reads in 116.558 CPU sec, 3.730 real sec
sorting block 39, #reads = 1082094 mem = 536878458 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288487, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.37, 106.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677196 sequences (100000237 bp) in 2.372 CPU sec, 2.646 real sec...
[M::process] read 676994 sequences (100000244 bp) in 1.699 CPU sec, 1.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288069, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.06, 105.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677284 reads in 205.954 CPU sec, 6.647 real sec
[M::mem_process_seqs] Processed 677352 reads in 116.421 CPU sec, 3.746 real sec
sorting block 40, #reads = 1082214 mem = 536878581 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288667, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.91, 108.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677120 sequences (100000141 bp) in 2.492 CPU sec, 2.978 real sec...
[M::process] read 676964 sequences (100000001 bp) in 1.571 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288224, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.41, 110.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677196 reads in 207.950 CPU sec, 6.756 real sec
[M::mem_process_seqs] Processed 676994 reads in 118.925 CPU sec, 3.857 real sec
sorting block 41, #reads = 1081103 mem = 536878632 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288396, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.89, 109.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676896 sequences (100000037 bp) in 2.519 CPU sec, 2.938 real sec...
[M::process] read 676908 sequences (100000221 bp) in 1.700 CPU sec, 1.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288397, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.73, 105.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677120 reads in 206.346 CPU sec, 6.634 real sec
[M::mem_process_seqs] Processed 676964 reads in 114.758 CPU sec, 3.659 real sec
sorting block 42, #reads = 1077576 mem = 536878515 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287453, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.03, 107.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1080334 mem = 536879519 peak = 874123264
[M::process] read 676916 sequences (100000021 bp) in 2.625 CPU sec, 2.941 real sec...
[M::process] read 676690 sequences (100000247 bp) in 1.729 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287857, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.55, 110.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 256, 939)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2755)
[M::mem_pestat] mean and std.dev: (359.55, 368.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3663)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676896 reads in 210.875 CPU sec, 6.807 real sec
[M::mem_process_seqs] Processed 676908 reads in 117.230 CPU sec, 3.757 real sec
sorting block 44, #reads = 1081306 mem = 536877892 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287766, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.84, 109.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 662, 3273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9317)
[M::mem_pestat] mean and std.dev: (1534.20, 1426.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12339)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676786 sequences (100000247 bp) in 2.565 CPU sec, 2.916 real sec...
[M::process] read 676924 sequences (100000158 bp) in 1.765 CPU sec, 1.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288101, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.97, 112.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676916 reads in 214.155 CPU sec, 6.887 real sec
[M::mem_process_seqs] Processed 676690 reads in 119.083 CPU sec, 3.805 real sec
sorting block 45, #reads = 1080747 mem = 536878908 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287490, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.76, 112.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677056 sequences (100000294 bp) in 2.794 CPU sec, 3.140 real sec...
[M::process] read 677058 sequences (100000290 bp) in 1.687 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287958, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.88, 111.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676786 reads in 213.284 CPU sec, 6.862 real sec
[M::mem_process_seqs] Processed 676924 reads in 119.263 CPU sec, 3.825 real sec
sorting block 46, #reads = 1082024 mem = 536881510 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288090, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.71, 109.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677224 sequences (100000261 bp) in 2.614 CPU sec, 2.919 real sec...
[M::process] read 677114 sequences (100000097 bp) in 1.755 CPU sec, 1.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288071, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.08, 108.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677056 reads in 212.422 CPU sec, 6.841 real sec
sorting block 47, #reads = 1079424 mem = 536879574 peak = 874123264
[M::mem_process_seqs] Processed 677058 reads in 119.697 CPU sec, 3.834 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287905, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.87, 107.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1082630 mem = 536878254 peak = 874123264
[M::process] read 676986 sequences (100000188 bp) in 2.888 CPU sec, 3.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287857, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.61, 110.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677010 sequences (100000260 bp) in 1.828 CPU sec, 1.832 real sec...
[M::mem_process_seqs] Processed 677224 reads in 204.794 CPU sec, 6.668 real sec
[M::mem_process_seqs] Processed 677114 reads in 117.398 CPU sec, 3.822 real sec
sorting block 49, #reads = 1082020 mem = 536879828 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287711, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.24, 110.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677002 sequences (100000180 bp) in 2.765 CPU sec, 3.047 real sec...
[M::process] read 676848 sequences (100000293 bp) in 1.825 CPU sec, 1.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287827, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.50, 106.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676986 reads in 207.816 CPU sec, 6.697 real sec
sorting block 50, #reads = 1077073 mem = 536878751 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287999, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.05, 105.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 87, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (103.60, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676890 sequences (100000170 bp) in 2.255 CPU sec, 2.309 real sec...
[M::mem_process_seqs] Processed 677002 reads in 111.810 CPU sec, 3.630 real sec
[M::mem_process_seqs] Processed 677010 reads in 271.740 CPU sec, 8.767 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287791, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.07, 108.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1080421 mem = 536879991 peak = 874123264
[M::process] read 676934 sequences (100000048 bp) in 2.686 CPU sec, 2.999 real sec...
[M::process] read 676812 sequences (100000228 bp) in 1.597 CPU sec, 1.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287539, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.03, 109.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676848 reads in 225.635 CPU sec, 7.260 real sec
[M::mem_process_seqs] Processed 676890 reads in 121.167 CPU sec, 3.887 real sec
sorting block 52, #reads = 1082150 mem = 536880509 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288177, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.55, 110.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676750 sequences (100000114 bp) in 2.366 CPU sec, 2.598 real sec...
[M::process] read 677084 sequences (100000281 bp) in 1.694 CPU sec, 1.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287819, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.49, 111.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676934 reads in 214.357 CPU sec, 6.909 real sec
[M::mem_process_seqs] Processed 676812 reads in 121.230 CPU sec, 3.933 real sec
sorting block 53, #reads = 1082019 mem = 536879030 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287670, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.07, 112.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676906 sequences (100000221 bp) in 2.586 CPU sec, 2.895 real sec...
[M::process] read 677132 sequences (100000268 bp) in 1.855 CPU sec, 1.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287708, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.19, 111.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 18, 35)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 101)
[M::mem_pestat] mean and std.dev: (17.42, 15.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 134)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676750 reads in 206.415 CPU sec, 6.661 real sec
[M::mem_process_seqs] Processed 677084 reads in 118.032 CPU sec, 3.812 real sec
sorting block 54, #reads = 1080237 mem = 536881084 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287774, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.79, 113.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1080455 mem = 536880490 peak = 874123264
[M::process] read 677014 sequences (100000298 bp) in 2.840 CPU sec, 3.094 real sec...
[M::process] read 676744 sequences (100000029 bp) in 1.830 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288371, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.01, 108.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676906 reads in 211.028 CPU sec, 6.787 real sec
[M::mem_process_seqs] Processed 677132 reads in 117.959 CPU sec, 3.773 real sec
sorting block 56, #reads = 1082063 mem = 536878100 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288813, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.53, 108.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676926 sequences (100000127 bp) in 2.653 CPU sec, 2.942 real sec...
[M::process] read 676994 sequences (100000298 bp) in 1.704 CPU sec, 1.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287936, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.84, 111.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677014 reads in 206.770 CPU sec, 6.658 real sec
[M::mem_process_seqs] Processed 676744 reads in 117.301 CPU sec, 3.764 real sec
sorting block 57, #reads = 1082142 mem = 536880016 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287985, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.41, 109.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677170 sequences (100000289 bp) in 2.810 CPU sec, 3.123 real sec...
[M::process] read 676838 sequences (100000178 bp) in 1.633 CPU sec, 1.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287517, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.47, 107.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676926 reads in 213.683 CPU sec, 6.869 real sec
[M::mem_process_seqs] Processed 676994 reads in 119.402 CPU sec, 3.825 real sec
sorting block 58, #reads = 1080869 mem = 536879347 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288362, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.88, 109.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676936 sequences (100000022 bp) in 2.453 CPU sec, 2.686 real sec...
[M::process] read 676694 sequences (100000140 bp) in 1.785 CPU sec, 1.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287926, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.05, 110.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 181, 2908)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8546)
[M::mem_pestat] mean and std.dev: (880.80, 1620.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11365)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677170 reads in 208.841 CPU sec, 6.704 real sec
[M::mem_process_seqs] Processed 676838 reads in 115.382 CPU sec, 3.713 real sec
sorting block 59, #reads = 1082285 mem = 536877593 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288318, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.50, 111.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1082943 mem = 536877150 peak = 874123264
[M::process] read 676700 sequences (100000073 bp) in 2.822 CPU sec, 3.259 real sec...
[M::process] read 312380 sequences (46169091 bp) in 1.125 CPU sec, 1.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287662, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.18, 114.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 994, 1116)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3198)
[M::mem_pestat] mean and std.dev: (771.58, 833.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4239)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676936 reads in 214.042 CPU sec, 6.913 real sec
[M::mem_process_seqs] Processed 676694 reads in 120.590 CPU sec, 3.882 real sec
sorting block 61, #reads = 1082659 mem = 536877579 peak = 874123264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288011, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.51, 115.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 91, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048)
[M::mem_pestat] mean and std.dev: (267.67, 383.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2730)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 132745, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.25, 116.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676700 reads in 152.236 CPU sec, 4.993 real sec
[M::mem_process_seqs] Processed 312380 reads in 56.838 CPU sec, 1.872 real sec
sorting block 62, #reads = 1082311 mem = 536878510 peak = 874123264
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67996868
overall: 69293600768 mem 13109.024 user 870.176 sys 482.860 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L003\tPU:L003\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T21-D0003376_R1.fastq.gz 0004.T21-D0003376_R2.fastq.gz
[main] Real time: 482.891 sec; CPU: 13979.204 sec
sorting block 63, #reads = 366255 mem = 181646789 peak = 874123264
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2729413402
output reads: 68521456
bam_mem_sort: 64 calls 59.833 user 1.464 sys 62.147 real
bam_write: 64 calls 44.847 user 3.958 sys 78.770 real
execute: 1 calls 29.215 user 2.863 sys 517.127 real
merge_files: 1 calls 29.192 user 2.837 sys 34.049 real
parse_chunk: 1014 calls 49.914 user 14.744 sys 73.305 real
read_chunk: 2730 calls 34.548 user 14.446 sys 376.187 real
sort_block: 1 calls 24.445 user 13.366 sys 359.971 real
write_chunk: 1714 calls 28.410 user 2.514 sys 32.700 real
overall: 2047209472 mem 841.331 user 65.353 sys 517.143 real
ls: cannot access '*.cram': No such file or directory