.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T18-D0003255;L006;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b9/c1aa3380aedcfc89c3f339ac707eba/.command.log
Size: 120.7 KB
Task: DAQ:SENTIEON_BWAMEM (T18-D0003255;L006;0001)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/43/bee6fe602e542585ff2c635192033d/T18-D0003255/0001.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b9/c1aa3380aedcfc89c3f339ac707eba/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/43/bee6fe602e542585ff2c635192033d/T18-D0003255/0001.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b9/c1aa3380aedcfc89c3f339ac707eba/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1944.681 CPU sec, 376.787 real sec
[M::process] read 674148 sequences (100000228 bp) in 0.885 CPU sec, 0.890 real sec...
[M::process] read 673874 sequences (100000059 bp) in 1.356 CPU sec, 1.366 real sec...
[M::process] read 673840 sequences (100000252 bp) in 1.301 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286430, 14, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (249.55, 122.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 48, 831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2487)
[M::mem_pestat] mean and std.dev: (421.07, 545.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3315)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673748 sequences (100000136 bp) in 1.169 CPU sec, 1.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286473, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.79, 126.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286970, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (252.10, 124.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 29, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (157.30, 233.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674148 reads in 286.831 CPU sec, 9.273 real sec
[M::mem_process_seqs] Processed 673840 reads in 177.867 CPU sec, 5.849 real sec
[M::mem_process_seqs] Processed 673874 reads in 185.896 CPU sec, 6.139 real sec
sorting block 0, #reads = 1080419 mem = 536878594 peak = 597389312
[M::process] read 673592 sequences (100000244 bp) in 1.785 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286063, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.25, 127.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 400, 1447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4305)
[M::mem_pestat] mean and std.dev: (514.50, 628.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5734)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673416 sequences (100000078 bp) in 1.160 CPU sec, 1.224 real sec...
[M::process] read 673418 sequences (100000288 bp) in 1.030 CPU sec, 1.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286227, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.27, 128.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 384, 1422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4240)
[M::mem_pestat] mean and std.dev: (534.80, 604.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5649)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673748 reads in 171.346 CPU sec, 5.491 real sec
sorting block 1, #reads = 1080684 mem = 536877957 peak = 779841536
[M::mem_process_seqs] Processed 673592 reads in 97.354 CPU sec, 3.127 real sec
sorting block 2, #reads = 1080176 mem = 536878286 peak = 862187520
[M::process] read 673338 sequences (100000294 bp) in 1.983 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285862, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (261.12, 131.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 70, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427)
[M::mem_pestat] mean and std.dev: (227.15, 264.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1889)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673366 sequences (100000196 bp) in 1.020 CPU sec, 1.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285562, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (262.78, 133.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 75, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (147.70, 219.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285618, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.84, 135.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 54, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2115)
[M::mem_pestat] mean and std.dev: (471.36, 696.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3258)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673416 reads in 253.416 CPU sec, 8.130 real sec
[M::mem_process_seqs] Processed 673418 reads in 176.622 CPU sec, 5.705 real sec
[M::mem_process_seqs] Processed 673338 reads in 184.581 CPU sec, 5.990 real sec
sorting block 3, #reads = 1080341 mem = 536879076 peak = 862187520
[M::process] read 673254 sequences (100000126 bp) in 2.117 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285080, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.72, 137.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1080134 mem = 536877915 peak = 862187520
[M::process] read 673176 sequences (100000090 bp) in 1.501 CPU sec, 1.696 real sec...
[M::process] read 673108 sequences (100000075 bp) in 1.010 CPU sec, 1.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285251, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.59, 140.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673366 reads in 176.438 CPU sec, 5.644 real sec
[M::mem_process_seqs] Processed 673254 reads in 98.939 CPU sec, 3.160 real sec
sorting block 5, #reads = 1079852 mem = 536880616 peak = 862187520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285253, 14, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.85, 141.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 213, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (199.21, 156.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1388)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673040 sequences (100000267 bp) in 2.137 CPU sec, 2.430 real sec...
[M::process] read 672942 sequences (100000025 bp) in 1.030 CPU sec, 1.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285495, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.93, 140.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 47, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338)
[M::mem_pestat] mean and std.dev: (48.20, 49.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673176 reads in 173.522 CPU sec, 5.553 real sec
[M::mem_process_seqs] Processed 673108 reads in 97.095 CPU sec, 3.133 real sec
sorting block 6, #reads = 1079869 mem = 536882157 peak = 862187520
[M::process] read 672806 sequences (100000143 bp) in 1.684 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284817, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.81, 142.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 122, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193)
[M::mem_pestat] mean and std.dev: (253.27, 434.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2923)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672750 sequences (100000173 bp) in 1.070 CPU sec, 1.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285175, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.29, 145.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285097, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.02, 146.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673040 reads in 252.805 CPU sec, 8.128 real sec
[M::mem_process_seqs] Processed 672942 reads in 175.825 CPU sec, 5.682 real sec
[M::mem_process_seqs] Processed 672806 reads in 183.285 CPU sec, 5.947 real sec
sorting block 7, #reads = 1079749 mem = 536877608 peak = 862187520
sorting block 8, #reads = 1079619 mem = 536880982 peak = 862187520
[M::process] read 673736 sequences (100000067 bp) in 2.202 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284933, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.49, 148.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 92, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (142.47, 184.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673940 sequences (100000110 bp) in 1.469 CPU sec, 1.600 real sec...
[M::process] read 673894 sequences (100000149 bp) in 1.040 CPU sec, 1.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286178, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.05, 128.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672750 reads in 173.014 CPU sec, 5.535 real sec
[M::mem_process_seqs] Processed 673736 reads in 95.679 CPU sec, 3.055 real sec
sorting block 9, #reads = 1079539 mem = 536879509 peak = 862187520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286426, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (253.12, 124.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673724 sequences (100000160 bp) in 2.199 CPU sec, 2.564 real sec...
[M::process] read 673564 sequences (100000254 bp) in 1.050 CPU sec, 1.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286029, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.36, 126.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673940 reads in 169.522 CPU sec, 5.430 real sec
[M::mem_process_seqs] Processed 673894 reads in 95.162 CPU sec, 3.057 real sec
sorting block 10, #reads = 1080269 mem = 536879042 peak = 862187520
[M::process] read 673478 sequences (100000112 bp) in 1.658 CPU sec, 1.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285884, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (256.64, 127.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673388 sequences (100000202 bp) in 1.080 CPU sec, 1.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285872, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.65, 129.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 25, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300)
[M::mem_pestat] mean and std.dev: (36.64, 41.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 399)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285367, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (260.59, 130.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673724 reads in 250.646 CPU sec, 8.067 real sec
sorting block 11, #reads = 1080533 mem = 536880294 peak = 862187520
[M::mem_process_seqs] Processed 673564 reads in 174.485 CPU sec, 5.638 real sec
[M::mem_process_seqs] Processed 673478 reads in 182.653 CPU sec, 5.931 real sec
sorting block 12, #reads = 1080093 mem = 536878844 peak = 904306688
[M::process] read 673506 sequences (100000235 bp) in 2.359 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285883, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.10, 134.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 31, 165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473)
[M::mem_pestat] mean and std.dev: (58.85, 60.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673360 sequences (100000175 bp) in 1.510 CPU sec, 1.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285282, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.90, 135.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 72, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (126.18, 112.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673230 sequences (100000297 bp) in 1.050 CPU sec, 1.043 real sec...
[M::mem_process_seqs] Processed 673388 reads in 181.353 CPU sec, 5.820 real sec
sorting block 13, #reads = 1079724 mem = 536878233 peak = 904306688
[M::mem_process_seqs] Processed 673506 reads in 104.740 CPU sec, 3.355 real sec
sorting block 14, #reads = 1079774 mem = 536878319 peak = 904306688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285781, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.51, 136.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673154 sequences (100000009 bp) in 2.205 CPU sec, 2.518 real sec...
[M::process] read 673118 sequences (100000079 bp) in 1.120 CPU sec, 1.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285621, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.37, 139.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673360 reads in 175.642 CPU sec, 5.631 real sec
[M::mem_process_seqs] Processed 673230 reads in 98.485 CPU sec, 3.172 real sec
sorting block 15, #reads = 1079928 mem = 536880699 peak = 904306688
[M::process] read 672896 sequences (100000033 bp) in 1.740 CPU sec, 1.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285497, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.68, 139.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672934 sequences (100000187 bp) in 1.110 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285024, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.88, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 148, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438)
[M::mem_pestat] mean and std.dev: (98.00, 76.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 583)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284636, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.35, 142.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 14, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954)
[M::mem_pestat] mean and std.dev: (205.00, 300.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2601)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673154 reads in 255.591 CPU sec, 8.223 real sec
[M::mem_process_seqs] Processed 673118 reads in 179.348 CPU sec, 5.790 real sec
[M::mem_process_seqs] Processed 672896 reads in 186.898 CPU sec, 6.058 real sec
sorting block 16, #reads = 1079548 mem = 536880545 peak = 904306688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285061, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.48, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672898 sequences (100000145 bp) in 2.483 CPU sec, 2.840 real sec...
[M::mem_process_seqs] Processed 672934 reads in 94.723 CPU sec, 3.133 real sec
sorting block 17, #reads = 1079795 mem = 536879652 peak = 904306688
[M::process] read 672770 sequences (100000220 bp) in 2.362 CPU sec, 2.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285923, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.57, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 81, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342)
[M::mem_pestat] mean and std.dev: (85.83, 73.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 448)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673394 sequences (100000203 bp) in 1.159 CPU sec, 1.154 real sec...
[M::process] read 673962 sequences (100000093 bp) in 1.070 CPU sec, 1.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285416, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.86, 148.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 85, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (111.60, 109.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672898 reads in 174.375 CPU sec, 5.572 real sec
sorting block 18, #reads = 1079424 mem = 536881180 peak = 904306688
[M::mem_process_seqs] Processed 672770 reads in 98.590 CPU sec, 3.160 real sec
sorting block 19, #reads = 1079815 mem = 536881152 peak = 929918976
[M::process] read 673866 sequences (100000058 bp) in 2.372 CPU sec, 2.681 real sec...
[M::process] read 673686 sequences (100000124 bp) in 1.110 CPU sec, 1.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285977, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (252.98, 125.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673962 reads in 88.625 CPU sec, 2.830 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286078, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.54, 134.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673588 sequences (100000233 bp) in 1.049 CPU sec, 1.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285963, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.93, 126.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673394 reads in 256.940 CPU sec, 8.234 real sec
[M::mem_process_seqs] Processed 673866 reads in 103.785 CPU sec, 3.369 real sec
sorting block 20, #reads = 1079463 mem = 536880125 peak = 929918976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286027, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.17, 128.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673592 sequences (100000186 bp) in 2.445 CPU sec, 2.823 real sec...
[M::process] read 673460 sequences (100000014 bp) in 1.130 CPU sec, 1.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285848, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.51, 129.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673686 reads in 173.281 CPU sec, 5.556 real sec
sorting block 21, #reads = 1080253 mem = 536883934 peak = 929918976
[M::mem_process_seqs] Processed 673588 reads in 97.580 CPU sec, 3.133 real sec
sorting block 22, #reads = 1079929 mem = 536878498 peak = 937689088
[M::process] read 673414 sequences (100000056 bp) in 1.733 CPU sec, 1.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285219, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.86, 131.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 406, 1216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3452)
[M::mem_pestat] mean and std.dev: (636.73, 727.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4570)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673414 sequences (100000198 bp) in 1.140 CPU sec, 1.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285394, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.19, 133.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 35, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246)
[M::mem_pestat] mean and std.dev: (36.50, 26.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 320)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285480, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.47, 135.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673592 reads in 260.372 CPU sec, 8.396 real sec
[M::mem_process_seqs] Processed 673460 reads in 183.264 CPU sec, 5.942 real sec
[M::mem_process_seqs] Processed 673414 reads in 191.544 CPU sec, 6.238 real sec
sorting block 23, #reads = 1079844 mem = 536876667 peak = 937689088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285694, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 673260 sequences (100000275 bp) in 2.458 CPU sec, 2.876 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (263.99, 134.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1080145 mem = 536878219 peak = 937689088
[M::process] read 673170 sequences (100000174 bp) in 1.604 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285648, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.75, 139.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (70, 98, 911)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593)
[M::mem_pestat] mean and std.dev: (388.64, 437.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3434)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673028 sequences (100000161 bp) in 1.160 CPU sec, 1.158 real sec...
[M::mem_process_seqs] Processed 673414 reads in 179.587 CPU sec, 5.801 real sec
[M::mem_process_seqs] Processed 673260 reads in 99.882 CPU sec, 3.188 real sec
sorting block 25, #reads = 1080318 mem = 536878873 peak = 937689088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285391, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.92, 140.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 173, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367)
[M::mem_pestat] mean and std.dev: (123.20, 66.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673072 sequences (100000162 bp) in 2.240 CPU sec, 2.553 real sec...
[M::process] read 672858 sequences (100000102 bp) in 1.120 CPU sec, 1.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285082, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.06, 142.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673170 reads in 178.729 CPU sec, 5.738 real sec
[M::mem_process_seqs] Processed 673028 reads in 101.482 CPU sec, 3.268 real sec
sorting block 26, #reads = 1079994 mem = 536880379 peak = 937689088
[M::process] read 672960 sequences (100000185 bp) in 1.804 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284666, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.31, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 15, 67)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175)
[M::mem_pestat] mean and std.dev: (21.73, 17.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 229)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672700 sequences (100000201 bp) in 1.140 CPU sec, 1.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284857, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.51, 146.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284416, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.87, 145.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673072 reads in 261.440 CPU sec, 8.419 real sec
[M::mem_process_seqs] Processed 672960 reads in 182.086 CPU sec, 5.883 real sec
[M::mem_process_seqs] Processed 672858 reads in 189.688 CPU sec, 6.154 real sec
sorting block 27, #reads = 1079393 mem = 536879333 peak = 937689088
sorting block 28, #reads = 1079184 mem = 536879219 peak = 937689088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284913, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.97, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 649, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1885)
[M::mem_pestat] mean and std.dev: (361.73, 270.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2482)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673150 sequences (100000233 bp) in 2.588 CPU sec, 3.002 real sec...
[M::mem_process_seqs] Processed 672700 reads in 95.703 CPU sec, 3.178 real sec
sorting block 29, #reads = 1079088 mem = 536876932 peak = 937689088
[M::process] read 673986 sequences (100000027 bp) in 2.325 CPU sec, 2.701 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285778, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.22, 141.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 67, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023)
[M::mem_pestat] mean and std.dev: (302.78, 416.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2689)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673846 sequences (100000256 bp) in 1.153 CPU sec, 1.165 real sec...
[M::process] read 673782 sequences (100000009 bp) in 1.070 CPU sec, 1.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286182, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (251.19, 122.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673150 reads in 172.517 CPU sec, 5.505 real sec
[M::mem_process_seqs] Processed 673986 reads in 95.116 CPU sec, 3.040 real sec
sorting block 30, #reads = 1079619 mem = 536879499 peak = 937689088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286284, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (252.77, 123.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673616 sequences (100000010 bp) in 2.491 CPU sec, 2.824 real sec...
[M::process] read 673208 sequences (100000284 bp) in 1.100 CPU sec, 1.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286374, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.78, 126.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 13, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213)
[M::mem_pestat] mean and std.dev: (28.79, 32.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673846 reads in 171.599 CPU sec, 5.495 real sec
[M::mem_process_seqs] Processed 673782 reads in 96.199 CPU sec, 3.086 real sec
sorting block 31, #reads = 1080457 mem = 536882413 peak = 937689088
[M::process] read 673410 sequences (100000071 bp) in 1.688 CPU sec, 1.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285617, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.93, 128.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673284 sequences (100000159 bp) in 1.137 CPU sec, 1.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285480, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.39, 137.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 91, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437)
[M::mem_pestat] mean and std.dev: (112.44, 74.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285531, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (260.75, 130.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 13, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120)
[M::mem_pestat] mean and std.dev: (146.42, 221.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1492)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673616 reads in 256.171 CPU sec, 8.251 real sec
[M::mem_process_seqs] Processed 673208 reads in 180.103 CPU sec, 5.829 real sec
[M::mem_process_seqs] Processed 673410 reads in 187.561 CPU sec, 6.094 real sec
sorting block 32, #reads = 1080530 mem = 536878163 peak = 937689088
sorting block 33, #reads = 1079815 mem = 536877851 peak = 949493760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284906, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.88, 133.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673304 sequences (100000072 bp) in 2.601 CPU sec, 3.013 real sec...
[M::mem_process_seqs] Processed 673284 reads in 97.247 CPU sec, 3.217 real sec
sorting block 34, #reads = 1079916 mem = 536882236 peak = 949493760
[M::process] read 673182 sequences (100000283 bp) in 2.370 CPU sec, 2.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285612, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (263.58, 132.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 65, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242)
[M::mem_pestat] mean and std.dev: (39.56, 37.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 320)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673120 sequences (100000084 bp) in 1.073 CPU sec, 1.230 real sec...
[M::process] read 673064 sequences (100000209 bp) in 1.100 CPU sec, 1.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285118, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.45, 137.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673304 reads in 179.550 CPU sec, 5.742 real sec
[M::mem_process_seqs] Processed 673182 reads in 101.097 CPU sec, 3.239 real sec
sorting block 35, #reads = 1080287 mem = 536877712 peak = 949493760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285038, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.13, 137.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672972 sequences (100000220 bp) in 2.315 CPU sec, 2.597 real sec...
[M::process] read 672932 sequences (100000074 bp) in 1.140 CPU sec, 1.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285070, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.26, 139.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673120 reads in 180.094 CPU sec, 5.778 real sec
[M::mem_process_seqs] Processed 673064 reads in 101.216 CPU sec, 3.267 real sec
sorting block 36, #reads = 1079946 mem = 536878210 peak = 949493760
[M::process] read 672732 sequences (100000162 bp) in 1.770 CPU sec, 2.006 real sec...
[M::process] read 672738 sequences (100000287 bp) in 1.130 CPU sec, 1.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285005, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.35, 147.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672732 reads in 92.875 CPU sec, 2.987 real sec
sorting block 37, #reads = 1079667 mem = 536880074 peak = 949493760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284875, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.15, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672668 sequences (100000000 bp) in 1.110 CPU sec, 1.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285226, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.64, 143.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 43, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253)
[M::mem_pestat] mean and std.dev: (33.73, 33.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 336)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672972 reads in 269.896 CPU sec, 8.672 real sec
[M::mem_process_seqs] Processed 672932 reads in 191.011 CPU sec, 6.158 real sec
sorting block 38, #reads = 1079465 mem = 536880068 peak = 949493760
[M::process] read 674164 sequences (100000180 bp) in 2.071 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284805, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.30, 148.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674158 sequences (100000088 bp) in 1.110 CPU sec, 1.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285204, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.07, 148.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672738 reads in 174.497 CPU sec, 5.597 real sec
[M::mem_process_seqs] Processed 672668 reads in 96.645 CPU sec, 3.116 real sec
sorting block 39, #reads = 1079641 mem = 536878861 peak = 949493760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285605, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.12, 128.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674070 sequences (100000096 bp) in 1.992 CPU sec, 2.266 real sec...
[M::process] read 673998 sequences (100000299 bp) in 1.110 CPU sec, 1.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286123, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (252.01, 126.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674164 reads in 172.805 CPU sec, 5.543 real sec
[M::mem_process_seqs] Processed 674158 reads in 97.061 CPU sec, 3.124 real sec
sorting block 40, #reads = 1078983 mem = 536878836 peak = 949493760
sorting block 41, #reads = 1080326 mem = 536877923 peak = 981405696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285609, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (253.83, 127.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673900 sequences (100000274 bp) in 2.134 CPU sec, 2.375 real sec...
[M::process] read 673602 sequences (100000132 bp) in 1.200 CPU sec, 1.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286131, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.02, 128.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674070 reads in 174.138 CPU sec, 5.571 real sec
[M::mem_process_seqs] Processed 673998 reads in 96.653 CPU sec, 3.110 real sec
sorting block 42, #reads = 1080811 mem = 536879813 peak = 981405696
[M::process] read 673580 sequences (100000148 bp) in 2.102 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285228, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.21, 131.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673554 sequences (100000208 bp) in 1.100 CPU sec, 1.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285503, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.21, 132.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673900 reads in 177.184 CPU sec, 5.676 real sec
[M::mem_process_seqs] Processed 673602 reads in 99.360 CPU sec, 3.202 real sec
sorting block 43, #reads = 1080724 mem = 536880884 peak = 981405696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285041, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.18, 135.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 543, 1686)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4926)
[M::mem_pestat] mean and std.dev: (461.57, 545.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6546)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673386 sequences (100000041 bp) in 2.281 CPU sec, 2.547 real sec...
[M::process] read 673272 sequences (100000215 bp) in 1.450 CPU sec, 1.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285226, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.83, 137.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673580 reads in 181.116 CPU sec, 5.786 real sec
[M::mem_process_seqs] Processed 673554 reads in 101.733 CPU sec, 3.268 real sec
sorting block 44, #reads = 1079977 mem = 536878370 peak = 981405696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285074, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (265.88, 138.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 54, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1883)
[M::mem_pestat] mean and std.dev: (196.25, 275.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2509)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673256 sequences (100000222 bp) in 2.355 CPU sec, 2.611 real sec...
[M::process] read 673266 sequences (100000142 bp) in 1.120 CPU sec, 1.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284851, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.10, 139.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 148, 3133)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9393)
[M::mem_pestat] mean and std.dev: (992.14, 1362.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12523)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673386 reads in 181.646 CPU sec, 5.821 real sec
sorting block 45, #reads = 1079977 mem = 536880230 peak = 981405696
[M::mem_process_seqs] Processed 673272 reads in 102.140 CPU sec, 3.297 real sec
sorting block 46, #reads = 1079541 mem = 536880730 peak = 981405696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285207, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.83, 138.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 552, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893)
[M::mem_pestat] mean and std.dev: (500.17, 517.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2570)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673162 sequences (100000036 bp) in 2.166 CPU sec, 2.448 real sec...
[M::process] read 673116 sequences (100000180 bp) in 1.130 CPU sec, 1.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285288, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.86, 140.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673256 reads in 176.346 CPU sec, 5.644 real sec
[M::mem_process_seqs] Processed 673266 reads in 99.643 CPU sec, 3.208 real sec
sorting block 47, #reads = 1080115 mem = 536878429 peak = 981405696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284836, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.82, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 242, 999)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2939)
[M::mem_pestat] mean and std.dev: (406.30, 438.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3909)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673078 sequences (100000182 bp) in 2.430 CPU sec, 2.641 real sec...
[M::process] read 673438 sequences (100000135 bp) in 1.320 CPU sec, 1.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284968, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.36, 144.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673162 reads in 178.641 CPU sec, 5.710 real sec
[M::mem_process_seqs] Processed 673116 reads in 100.012 CPU sec, 3.206 real sec
sorting block 48, #reads = 1079714 mem = 536877027 peak = 981405696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285084, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (277.54, 148.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674134 sequences (100000202 bp) in 2.280 CPU sec, 2.564 real sec...
[M::process] read 674196 sequences (100000295 bp) in 1.130 CPU sec, 1.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285719, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.11, 141.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673078 reads in 175.473 CPU sec, 5.613 real sec
[M::mem_process_seqs] Processed 673438 reads in 97.680 CPU sec, 3.135 real sec
sorting block 49, #reads = 1079955 mem = 536878839 peak = 981405696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286326, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (250.90, 125.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 117, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (163.47, 200.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673910 sequences (100000000 bp) in 2.250 CPU sec, 2.554 real sec...
[M::process] read 673836 sequences (100000009 bp) in 1.150 CPU sec, 1.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286027, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.24, 127.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674134 reads in 171.120 CPU sec, 5.480 real sec
sorting block 50, #reads = 1079923 mem = 536881285 peak = 981405696
[M::mem_process_seqs] Processed 674196 reads in 96.281 CPU sec, 3.090 real sec
sorting block 51, #reads = 1080225 mem = 536877188 peak = 986259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286236, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.07, 128.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673740 sequences (100000282 bp) in 2.234 CPU sec, 2.518 real sec...
[M::process] read 673628 sequences (100000274 bp) in 1.130 CPU sec, 1.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285688, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.34, 129.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673910 reads in 174.878 CPU sec, 5.592 real sec
[M::mem_process_seqs] Processed 673836 reads in 98.678 CPU sec, 3.164 real sec
sorting block 52, #reads = 1080384 mem = 536877454 peak = 986259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285324, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.87, 131.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673570 sequences (100000024 bp) in 2.204 CPU sec, 2.482 real sec...
[M::process] read 673640 sequences (100000126 bp) in 1.126 CPU sec, 1.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285528, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.76, 134.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673740 reads in 176.846 CPU sec, 5.662 real sec
[M::mem_process_seqs] Processed 673628 reads in 99.886 CPU sec, 3.221 real sec
sorting block 53, #reads = 1080268 mem = 536880329 peak = 986259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284737, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.42, 137.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673480 sequences (100000282 bp) in 2.251 CPU sec, 2.536 real sec...
[M::process] read 673300 sequences (100000287 bp) in 1.500 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285006, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.68, 135.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673570 reads in 176.520 CPU sec, 5.638 real sec
[M::mem_process_seqs] Processed 673640 reads in 97.506 CPU sec, 3.128 real sec
sorting block 54, #reads = 1079454 mem = 536883095 peak = 986259456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284755, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.23, 139.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 78, 1521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4527)
[M::mem_pestat] mean and std.dev: (713.91, 1239.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6030)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673180 sequences (100000081 bp) in 2.610 CPU sec, 2.917 real sec...
[M::process] read 673226 sequences (100000147 bp) in 1.200 CPU sec, 1.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284965, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.61, 140.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673480 reads in 181.694 CPU sec, 5.812 real sec
sorting block 55, #reads = 1079450 mem = 536876359 peak = 986259456
[M::mem_process_seqs] Processed 673300 reads in 101.639 CPU sec, 3.260 real sec
sorting block 56, #reads = 1079870 mem = 536880295 peak = 992161792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284378, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.14, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 41, 69)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181)
[M::mem_pestat] mean and std.dev: (38.00, 29.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 237)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673160 sequences (100000254 bp) in 2.289 CPU sec, 2.583 real sec...
[M::process] read 672964 sequences (100000167 bp) in 1.140 CPU sec, 1.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284555, 12, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.88, 143.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 208, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809)
[M::mem_pestat] mean and std.dev: (299.25, 275.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2383)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673180 reads in 181.271 CPU sec, 5.800 real sec
[M::mem_process_seqs] Processed 673226 reads in 101.266 CPU sec, 3.252 real sec
sorting block 57, #reads = 1079355 mem = 536880516 peak = 992161792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284241, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.72, 145.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (82, 92, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861)
[M::mem_pestat] mean and std.dev: (389.12, 464.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2454)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673048 sequences (100000137 bp) in 2.302 CPU sec, 2.606 real sec...
[M::process] read 674068 sequences (100000128 bp) in 1.130 CPU sec, 1.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284708, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.49, 148.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 619, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1977)
[M::mem_pestat] mean and std.dev: (386.30, 387.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2635)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673160 reads in 182.016 CPU sec, 5.825 real sec
[M::mem_process_seqs] Processed 672964 reads in 100.808 CPU sec, 3.236 real sec
sorting block 58, #reads = 1079650 mem = 536880697 peak = 992161792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284122, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.04, 149.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674130 sequences (100000130 bp) in 2.163 CPU sec, 2.425 real sec...
[M::process] read 674052 sequences (100000085 bp) in 1.140 CPU sec, 1.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285785, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (253.17, 127.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673048 reads in 176.693 CPU sec, 5.655 real sec
[M::mem_process_seqs] Processed 674068 reads in 97.166 CPU sec, 3.125 real sec
sorting block 59, #reads = 1079711 mem = 536878027 peak = 992161792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285771, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (252.89, 126.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673864 sequences (100000130 bp) in 2.240 CPU sec, 2.541 real sec...
[M::process] read 673734 sequences (100000071 bp) in 1.130 CPU sec, 1.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285687, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.68, 128.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 188, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288)
[M::mem_pestat] mean and std.dev: (159.70, 161.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1710)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674130 reads in 177.747 CPU sec, 5.692 real sec
sorting block 60, #reads = 1079261 mem = 536878568 peak = 992161792
[M::mem_process_seqs] Processed 674052 reads in 103.648 CPU sec, 3.338 real sec
sorting block 61, #reads = 1080549 mem = 536880118 peak = 1013506048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285967, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.38, 129.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673554 sequences (100000187 bp) in 2.285 CPU sec, 2.594 real sec...
[M::process] read 673586 sequences (100000221 bp) in 1.150 CPU sec, 1.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285483, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.23, 132.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673864 reads in 180.939 CPU sec, 5.795 real sec
[M::mem_process_seqs] Processed 673734 reads in 104.946 CPU sec, 3.388 real sec
sorting block 62, #reads = 1080627 mem = 536880093 peak = 1013506048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285346, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.72, 134.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673554 sequences (100000044 bp) in 2.338 CPU sec, 2.669 real sec...
[M::process] read 673522 sequences (100000230 bp) in 1.200 CPU sec, 1.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285238, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (263.83, 136.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673554 reads in 181.676 CPU sec, 5.812 real sec
[M::mem_process_seqs] Processed 673586 reads in 103.617 CPU sec, 3.333 real sec
sorting block 63, #reads = 1080174 mem = 536878706 peak = 1013506048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285371, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (261.39, 134.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673258 sequences (100000097 bp) in 2.715 CPU sec, 3.018 real sec...
[M::process] read 673254 sequences (100000086 bp) in 1.320 CPU sec, 1.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284954, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.38, 137.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 48, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404)
[M::mem_pestat] mean and std.dev: (67.29, 73.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 527)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673554 reads in 186.553 CPU sec, 5.971 real sec
[M::mem_process_seqs] Processed 673522 reads in 110.907 CPU sec, 3.566 real sec
sorting block 64, #reads = 1080197 mem = 536878965 peak = 1013506048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284400, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.20, 141.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 331, 894)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520)
[M::mem_pestat] mean and std.dev: (584.87, 678.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3333)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673200 sequences (100000192 bp) in 2.612 CPU sec, 2.943 real sec...
[M::process] read 673086 sequences (100000037 bp) in 1.140 CPU sec, 1.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284636, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.86, 142.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 91, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572)
[M::mem_pestat] mean and std.dev: (83.50, 105.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673258 reads in 188.809 CPU sec, 6.046 real sec
sorting block 65, #reads = 1080186 mem = 536876897 peak = 1013506048
[M::mem_process_seqs] Processed 673254 reads in 108.589 CPU sec, 3.503 real sec
sorting block 66, #reads = 1079805 mem = 536878323 peak = 1015820288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284349, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.44, 143.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673102 sequences (100000209 bp) in 2.304 CPU sec, 2.611 real sec...
[M::process] read 672872 sequences (100000259 bp) in 1.150 CPU sec, 1.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283846, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.68, 146.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673200 reads in 190.960 CPU sec, 6.116 real sec
[M::mem_process_seqs] Processed 673086 reads in 108.753 CPU sec, 3.513 real sec
sorting block 67, #reads = 1079545 mem = 536877991 peak = 1015820288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284595, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.79, 148.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 208, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112)
[M::mem_pestat] mean and std.dev: (334.50, 314.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2806)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673424 sequences (100000091 bp) in 2.349 CPU sec, 2.675 real sec...
[M::process] read 674084 sequences (100000177 bp) in 1.160 CPU sec, 1.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284581, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (278.26, 149.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 697, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657)
[M::mem_pestat] mean and std.dev: (407.33, 298.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2137)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673102 reads in 187.783 CPU sec, 6.013 real sec
[M::mem_process_seqs] Processed 672872 reads in 107.531 CPU sec, 3.467 real sec
sorting block 68, #reads = 1079587 mem = 536881452 peak = 1015820288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285160, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (266.75, 141.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 36, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2063)
[M::mem_pestat] mean and std.dev: (373.81, 625.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2877)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673858 sequences (100000274 bp) in 2.285 CPU sec, 2.574 real sec...
[M::process] read 673910 sequences (100000164 bp) in 1.170 CPU sec, 1.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286065, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.48, 125.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673424 reads in 175.686 CPU sec, 5.634 real sec
[M::mem_process_seqs] Processed 674084 reads in 99.607 CPU sec, 3.202 real sec
sorting block 69, #reads = 1079203 mem = 536879399 peak = 1015820288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285893, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.03, 126.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 318, 1095)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3153)
[M::mem_pestat] mean and std.dev: (608.40, 682.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4182)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673808 sequences (100000285 bp) in 2.277 CPU sec, 2.568 real sec...
[M::process] read 673290 sequences (100000152 bp) in 1.140 CPU sec, 1.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285534, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.21, 128.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673858 reads in 175.089 CPU sec, 5.601 real sec
sorting block 70, #reads = 1079941 mem = 536878168 peak = 1015820288
[M::mem_process_seqs] Processed 673910 reads in 100.619 CPU sec, 3.234 real sec
sorting block 71, #reads = 1080215 mem = 536876196 peak = 1015844864
[M::process] read 673506 sequences (100000286 bp) in 2.373 CPU sec, 2.681 real sec...
[M::process] read 673414 sequences (100000194 bp) in 1.170 CPU sec, 1.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284771, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.19, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673290 reads in 96.398 CPU sec, 3.083 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285476, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.24, 132.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673320 sequences (100000084 bp) in 1.134 CPU sec, 1.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284823, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (262.98, 136.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673808 reads in 278.812 CPU sec, 8.937 real sec
sorting block 72, #reads = 1079853 mem = 536878052 peak = 1015844864
[M::mem_process_seqs] Processed 673506 reads in 116.485 CPU sec, 3.791 real sec
sorting block 73, #reads = 1080163 mem = 536881251 peak = 1015844864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284847, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (264.89, 137.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673296 sequences (100000145 bp) in 2.724 CPU sec, 3.132 real sec...
[M::process] read 673324 sequences (100000198 bp) in 1.170 CPU sec, 1.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284795, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.10, 138.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 81, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228)
[M::mem_pestat] mean and std.dev: (59.58, 35.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 295)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673414 reads in 186.859 CPU sec, 5.977 real sec
[M::mem_process_seqs] Processed 673320 reads in 107.397 CPU sec, 3.461 real sec
sorting block 74, #reads = 1080076 mem = 536878524 peak = 1015844864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284908, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.77, 138.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673130 sequences (100000290 bp) in 2.154 CPU sec, 2.505 real sec...
[M::process] read 673060 sequences (100000168 bp) in 1.160 CPU sec, 1.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284551, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.98, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 150, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (96.11, 82.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673296 reads in 186.468 CPU sec, 5.977 real sec
[M::mem_process_seqs] Processed 673324 reads in 107.564 CPU sec, 3.468 real sec
sorting block 75, #reads = 1079569 mem = 536879388 peak = 1015844864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283839, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.54, 147.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672820 sequences (100000037 bp) in 2.314 CPU sec, 2.618 real sec...
[M::process] read 672822 sequences (100000274 bp) in 1.190 CPU sec, 1.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284166, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.52, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673130 reads in 189.765 CPU sec, 6.074 real sec
[M::mem_process_seqs] Processed 673060 reads in 106.074 CPU sec, 3.421 real sec
sorting block 76, #reads = 1079845 mem = 536878654 peak = 1015844864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283707, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.19, 150.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672962 sequences (100000051 bp) in 2.063 CPU sec, 2.365 real sec...
[M::process] read 157232 sequences (23365991 bp) in 0.430 CPU sec, 0.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283932, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.03, 153.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 128, 8249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24681)
[M::mem_pestat] mean and std.dev: (2391.07, 3579.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32897)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672820 reads in 189.862 CPU sec, 6.116 real sec
sorting block 77, #reads = 1079750 mem = 536877741 peak = 1015844864
[M::mem_process_seqs] Processed 672822 reads in 106.474 CPU sec, 3.452 real sec
sorting block 78, #reads = 1078297 mem = 536878709 peak = 1099948032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284304, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (281.33, 152.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 66528, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (283.04, 154.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672962 reads in 118.144 CPU sec, 3.854 real sec
[M::mem_process_seqs] Processed 157232 reads in 31.896 CPU sec, 1.047 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 85679932
overall: 69674041344 mem 13069.770 user 766.612 sys 775.247 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L006\tPU:L006\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T18-D0003255_R1.fastq.gz 0001.T18-D0003255_R2.fastq.gz
[main] Real time: 775.278 sec; CPU: 13836.385 sec
sorting block 79, #reads = 995125 mem = 494923538 peak = 1099948032
uniq header count 1
merging 80 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3307465147
output reads: 86306620
bam_mem_sort: 80 calls 35.946 user 0.597 sys 36.727 real
bam_write: 80 calls 48.805 user 1.480 sys 50.854 real
execute: 1 calls 19.039 user 1.437 sys 797.914 real
merge_files: 1 calls 19.026 user 1.400 sys 21.444 real
parse_chunk: 1279 calls 41.797 user 12.946 sys 55.612 real
read_chunk: 3443 calls 36.857 user 30.856 sys 400.583 real
sort_block: 1 calls 29.934 user 30.127 sys 389.490 real
write_chunk: 2162 calls 18.700 user 1.133 sys 20.434 real
overall: 2378997760 mem 753.078 user 65.428 sys 797.931 real
ls: cannot access '*.cram': No such file or directory