This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2670.261 CPU sec, 95.612 real sec
[M::process] read 677674 sequences (100000152 bp) in 0.975 CPU sec, 0.981 real sec...
[M::process] read 677568 sequences (100000103 bp) in 1.694 CPU sec, 1.721 real sec...
[M::process] read 677424 sequences (100000193 bp) in 1.681 CPU sec, 1.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288808, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531)
[M::mem_pestat] mean and std.dev: (213.02, 90.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677138 sequences (100000035 bp) in 1.544 CPU sec, 1.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288925, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.55, 99.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288736, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.70, 102.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677674 reads in 327.760 CPU sec, 10.585 real sec
[M::mem_process_seqs] Processed 677568 reads in 198.296 CPU sec, 6.508 real sec
[M::mem_process_seqs] Processed 677424 reads in 206.054 CPU sec, 6.791 real sec
sorting block 0, #reads = 1082773 mem = 536879808 peak = 592474112
[M::process] read 677016 sequences (100000259 bp) in 1.880 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288448, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (224.21, 104.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676968 sequences (100000062 bp) in 1.549 CPU sec, 1.588 real sec...
[M::process] read 676936 sequences (100000095 bp) in 1.492 CPU sec, 1.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288440, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.74, 107.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677138 reads in 190.743 CPU sec, 6.085 real sec
sorting block 1, #reads = 1082952 mem = 536878127 peak = 885026816
[M::mem_process_seqs] Processed 677016 reads in 105.909 CPU sec, 3.368 real sec
sorting block 2, #reads = 1082307 mem = 536879874 peak = 885026816
[M::process] read 676762 sequences (100000054 bp) in 2.167 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288540, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.20, 109.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676764 sequences (100000112 bp) in 1.328 CPU sec, 1.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288220, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.81, 111.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287997, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.11, 109.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 225, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437)
[M::mem_pestat] mean and std.dev: (145.78, 92.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676968 reads in 270.443 CPU sec, 8.677 real sec
[M::mem_process_seqs] Processed 676762 reads in 192.289 CPU sec, 6.220 real sec
[M::mem_process_seqs] Processed 676936 reads in 201.092 CPU sec, 6.539 real sec
sorting block 3, #reads = 1082478 mem = 536881034 peak = 885026816
[M::process] read 676520 sequences (100000262 bp) in 2.188 CPU sec, 2.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287893, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.84, 112.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1082434 mem = 536877146 peak = 885026816
[M::process] read 676238 sequences (100000287 bp) in 2.006 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288070, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676238 sequences (100000122 bp) in 1.655 CPU sec, 1.661 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.44, 114.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676764 reads in 202.714 CPU sec, 6.498 real sec
[M::mem_process_seqs] Processed 676520 reads in 115.538 CPU sec, 3.675 real sec
sorting block 5, #reads = 1082154 mem = 536878174 peak = 885026816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288579, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.27, 117.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 125, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (107.38, 60.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676738 sequences (100000122 bp) in 2.677 CPU sec, 2.906 real sec...
[M::process] read 677522 sequences (100000140 bp) in 1.467 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288108, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.59, 118.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676238 reads in 198.808 CPU sec, 6.372 real sec
[M::mem_process_seqs] Processed 676238 reads in 112.889 CPU sec, 3.618 real sec
sorting block 6, #reads = 1082067 mem = 536879771 peak = 885026816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288328, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.20, 112.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677452 sequences (100000252 bp) in 2.151 CPU sec, 2.324 real sec...
[M::process] read 677332 sequences (100000082 bp) in 1.491 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288569, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (217.74, 96.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676738 reads in 200.866 CPU sec, 6.457 real sec
[M::mem_process_seqs] Processed 677522 reads in 114.213 CPU sec, 3.663 real sec
sorting block 7, #reads = 1081382 mem = 536878821 peak = 885026816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289144, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.92, 101.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 71, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314)
[M::mem_pestat] mean and std.dev: (186.00, 213.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1746)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676936 sequences (100000244 bp) in 2.083 CPU sec, 2.237 real sec...
[M::process] read 676984 sequences (100000012 bp) in 1.590 CPU sec, 1.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288894, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.79, 104.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677452 reads in 199.875 CPU sec, 6.442 real sec
[M::mem_process_seqs] Processed 677332 reads in 112.440 CPU sec, 3.609 real sec
sorting block 8, #reads = 1082591 mem = 536879876 peak = 885026816
sorting block 9, #reads = 1082912 mem = 536878630 peak = 895762432
[M::process] read 676964 sequences (100000050 bp) in 1.670 CPU sec, 1.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288326, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.58, 105.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676654 sequences (100000260 bp) in 1.383 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288668, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.75, 109.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288521, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.58, 108.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676936 reads in 293.380 CPU sec, 9.482 real sec
[M::mem_process_seqs] Processed 676964 reads in 204.697 CPU sec, 6.656 real sec
[M::mem_process_seqs] Processed 676984 reads in 212.738 CPU sec, 6.950 real sec
sorting block 10, #reads = 1082770 mem = 536879991 peak = 895762432
[M::process] read 676546 sequences (100000243 bp) in 2.413 CPU sec, 2.612 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288053, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.25, 110.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676628 sequences (100000175 bp) in 2.140 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288440, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.66, 112.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676496 sequences (100000026 bp) in 1.496 CPU sec, 1.498 real sec...
[M::mem_process_seqs] Processed 676654 reads in 209.405 CPU sec, 6.766 real sec
sorting block 11, #reads = 1082419 mem = 536878074 peak = 895762432
[M::mem_process_seqs] Processed 676546 reads in 116.307 CPU sec, 3.721 real sec
sorting block 12, #reads = 1082683 mem = 536877286 peak = 895762432
[M::process] read 676312 sequences (100000084 bp) in 2.128 CPU sec, 2.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288181, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.63, 115.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676378 sequences (100000102 bp) in 1.418 CPU sec, 1.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288074, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.37, 115.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 71, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259)
[M::mem_pestat] mean and std.dev: (54.18, 60.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 344)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676628 reads in 201.425 CPU sec, 6.477 real sec
[M::mem_process_seqs] Processed 676496 reads in 111.901 CPU sec, 3.600 real sec
sorting block 13, #reads = 1082170 mem = 536880216 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288011, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.25, 118.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677278 sequences (100000286 bp) in 1.986 CPU sec, 2.159 real sec...
[M::process] read 677544 sequences (100000260 bp) in 1.588 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288312, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.85, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676312 reads in 200.641 CPU sec, 6.467 real sec
[M::mem_process_seqs] Processed 676378 reads in 111.914 CPU sec, 3.596 real sec
sorting block 14, #reads = 1081818 mem = 536877677 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288140, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.08, 104.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677364 sequences (100000068 bp) in 2.371 CPU sec, 2.573 real sec...
[M::process] read 677284 sequences (100000070 bp) in 1.470 CPU sec, 1.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288411, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.47, 99.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677278 reads in 200.392 CPU sec, 6.444 real sec
[M::mem_process_seqs] Processed 677544 reads in 113.227 CPU sec, 3.639 real sec
sorting block 15, #reads = 1082014 mem = 536878330 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288700, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.62, 104.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677106 sequences (100000150 bp) in 2.166 CPU sec, 2.363 real sec...
sorting block 16, #reads = 1082305 mem = 536879827 peak = 895762432
[M::process] read 676950 sequences (100000152 bp) in 1.552 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287967, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.11, 105.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677364 reads in 202.915 CPU sec, 6.550 real sec
[M::mem_process_seqs] Processed 677284 reads in 115.144 CPU sec, 3.710 real sec
sorting block 17, #reads = 1082546 mem = 536880360 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288356, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.18, 106.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 38, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1583)
[M::mem_pestat] mean and std.dev: (186.92, 293.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2108)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676902 sequences (100000098 bp) in 2.671 CPU sec, 3.013 real sec...
[M::process] read 676916 sequences (100000231 bp) in 1.608 CPU sec, 1.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288700, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.05, 109.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677106 reads in 208.002 CPU sec, 6.736 real sec
[M::mem_process_seqs] Processed 676950 reads in 117.384 CPU sec, 3.844 real sec
sorting block 18, #reads = 1082442 mem = 536877801 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288482, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.85, 108.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 48, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (105.20, 129.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676632 sequences (100000057 bp) in 2.263 CPU sec, 2.438 real sec...
[M::process] read 676588 sequences (100000148 bp) in 1.628 CPU sec, 1.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288314, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.68, 112.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676902 reads in 209.046 CPU sec, 6.746 real sec
[M::mem_process_seqs] Processed 676916 reads in 116.328 CPU sec, 3.760 real sec
sorting block 19, #reads = 1082846 mem = 536877106 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288103, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.56, 113.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676422 sequences (100000081 bp) in 2.292 CPU sec, 2.479 real sec...
[M::process] read 676054 sequences (100000160 bp) in 1.547 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288030, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.54, 115.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676632 reads in 208.446 CPU sec, 6.733 real sec
[M::mem_process_seqs] Processed 676588 reads in 118.777 CPU sec, 3.868 real sec
sorting block 20, #reads = 1082970 mem = 536880476 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288247, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.01, 116.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082520 mem = 536880125 peak = 895762432
[M::process] read 676554 sequences (100000076 bp) in 2.354 CPU sec, 2.774 real sec...
[M::process] read 677484 sequences (100000089 bp) in 1.443 CPU sec, 1.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287768, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.75, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676422 reads in 209.678 CPU sec, 6.783 real sec
[M::mem_process_seqs] Processed 676054 reads in 117.812 CPU sec, 3.798 real sec
sorting block 22, #reads = 1082658 mem = 536880123 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287448, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.35, 117.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 73, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131)
[M::mem_pestat] mean and std.dev: (103.89, 145.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1507)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677366 sequences (100000201 bp) in 2.555 CPU sec, 2.840 real sec...
[M::process] read 677240 sequences (100000205 bp) in 1.542 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288938, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (216.51, 94.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676554 reads in 206.360 CPU sec, 6.655 real sec
[M::mem_process_seqs] Processed 677484 reads in 115.008 CPU sec, 3.699 real sec
sorting block 23, #reads = 1081081 mem = 536879235 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287809, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (220.04, 99.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676714 sequences (100000140 bp) in 2.283 CPU sec, 2.531 real sec...
[M::process] read 676880 sequences (100000125 bp) in 1.361 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288459, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.71, 102.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677366 reads in 205.783 CPU sec, 6.634 real sec
[M::mem_process_seqs] Processed 677240 reads in 113.938 CPU sec, 3.659 real sec
sorting block 24, #reads = 1082742 mem = 536880569 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288024, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.18, 111.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676768 sequences (100000262 bp) in 2.333 CPU sec, 2.714 real sec...
[M::process] read 676600 sequences (100000273 bp) in 1.333 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288058, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (226.39, 106.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676714 reads in 208.379 CPU sec, 6.751 real sec
[M::mem_process_seqs] Processed 676880 reads in 117.701 CPU sec, 3.803 real sec
sorting block 25, #reads = 1082218 mem = 536878463 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288405, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.47, 107.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1082184 mem = 536878281 peak = 895762432
[M::process] read 676622 sequences (100000211 bp) in 2.478 CPU sec, 2.723 real sec...
[M::process] read 676424 sequences (100000237 bp) in 1.593 CPU sec, 1.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288255, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.92, 112.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676768 reads in 211.397 CPU sec, 6.826 real sec
[M::process] read 676544 sequences (100000238 bp) in 1.812 CPU sec, 1.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288229, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.90, 111.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 53, 1703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5039)
[M::mem_pestat] mean and std.dev: (692.27, 784.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6707)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676622 reads in 109.782 CPU sec, 3.522 real sec
sorting block 27, #reads = 1082400 mem = 536880561 peak = 895762432
[M::mem_process_seqs] Processed 676600 reads in 277.746 CPU sec, 8.975 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288325, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.81, 114.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 922, 1123)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301)
[M::mem_pestat] mean and std.dev: (661.50, 642.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4390)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676238 sequences (100000188 bp) in 2.341 CPU sec, 2.584 real sec...
[M::process] read 676200 sequences (100000157 bp) in 1.596 CPU sec, 1.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288256, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.66, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 63, 119)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267)
[M::mem_pestat] mean and std.dev: (72.50, 52.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 341)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676424 reads in 224.487 CPU sec, 7.228 real sec
sorting block 28, #reads = 1082151 mem = 536880933 peak = 895762432
[M::mem_process_seqs] Processed 676544 reads in 120.693 CPU sec, 3.883 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288087, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.11, 119.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1082103 mem = 536881163 peak = 895762432
[M::process] read 676718 sequences (100000217 bp) in 2.321 CPU sec, 2.648 real sec...
[M::process] read 677842 sequences (100000149 bp) in 1.481 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287430, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.84, 119.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676238 reads in 212.052 CPU sec, 6.875 real sec
[M::mem_process_seqs] Processed 676200 reads in 119.014 CPU sec, 3.829 real sec
sorting block 30, #reads = 1082074 mem = 536878187 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288445, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.46, 113.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 448, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706)
[M::mem_pestat] mean and std.dev: (568.43, 558.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2804)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677686 sequences (100000230 bp) in 2.240 CPU sec, 2.534 real sec...
[M::process] read 677530 sequences (100000084 bp) in 1.446 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288542, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (212.72, 91.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676718 reads in 207.325 CPU sec, 6.705 real sec
[M::mem_process_seqs] Processed 677842 reads in 116.146 CPU sec, 3.743 real sec
sorting block 31, #reads = 1081246 mem = 536881029 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288854, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (215.69, 95.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 681)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677430 sequences (100000034 bp) in 2.546 CPU sec, 2.849 real sec...
[M::process] read 677200 sequences (100000229 bp) in 1.932 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288445, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558)
[M::mem_pestat] mean and std.dev: (216.96, 97.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677686 reads in 209.771 CPU sec, 6.748 real sec
[M::mem_process_seqs] Processed 677530 reads in 119.048 CPU sec, 3.823 real sec
sorting block 32, #reads = 1083036 mem = 536878327 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288439, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.98, 102.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677246 sequences (100000167 bp) in 2.341 CPU sec, 2.722 real sec...
sorting block 33, #reads = 1083365 mem = 536884850 peak = 895762432
[M::process] read 677000 sequences (100000202 bp) in 1.524 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288242, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.00, 104.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677430 reads in 211.389 CPU sec, 6.871 real sec
[M::mem_process_seqs] Processed 677200 reads in 119.116 CPU sec, 3.882 real sec
sorting block 34, #reads = 1083036 mem = 536878588 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288011, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.35, 106.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676976 sequences (100000055 bp) in 2.790 CPU sec, 3.065 real sec...
[M::process] read 676816 sequences (100000082 bp) in 1.540 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288329, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.64, 109.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677246 reads in 215.289 CPU sec, 7.041 real sec
[M::mem_process_seqs] Processed 677000 reads in 120.577 CPU sec, 4.011 real sec
sorting block 35, #reads = 1082508 mem = 536877850 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287821, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.70, 110.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676902 sequences (100000139 bp) in 2.095 CPU sec, 2.387 real sec...
[M::process] read 676652 sequences (100000095 bp) in 1.779 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288502, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.68, 111.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676976 reads in 208.749 CPU sec, 6.747 real sec
[M::mem_process_seqs] Processed 676816 reads in 120.977 CPU sec, 3.952 real sec
sorting block 36, #reads = 1082297 mem = 536879599 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288329, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.49, 112.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676562 sequences (100000265 bp) in 2.755 CPU sec, 2.962 real sec...
[M::process] read 677828 sequences (100000190 bp) in 1.622 CPU sec, 1.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288284, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.65, 115.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 107, 640)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742)
[M::mem_pestat] mean and std.dev: (167.54, 190.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2293)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676902 reads in 213.401 CPU sec, 6.921 real sec
[M::mem_process_seqs] Processed 676652 reads in 119.484 CPU sec, 3.870 real sec
sorting block 37, #reads = 1082835 mem = 536877855 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288240, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.57, 116.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1082505 mem = 536876325 peak = 895762432
[M::process] read 677800 sequences (100000291 bp) in 2.402 CPU sec, 2.655 real sec...
[M::process] read 677532 sequences (100000031 bp) in 1.688 CPU sec, 1.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288560, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (208.61, 85.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676562 reads in 207.844 CPU sec, 6.741 real sec
[M::mem_process_seqs] Processed 677828 reads in 116.412 CPU sec, 3.816 real sec
sorting block 39, #reads = 1082470 mem = 536876644 peak = 895762432
[M::process] read 677404 sequences (100000011 bp) in 2.698 CPU sec, 2.974 real sec...
[M::process] read 677412 sequences (100000063 bp) in 1.715 CPU sec, 1.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288707, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.31, 100.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677532 reads in 110.173 CPU sec, 3.517 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288823, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549)
[M::mem_pestat] mean and std.dev: (215.34, 94.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (17, 34, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 203)
[M::mem_pestat] mean and std.dev: (35.20, 27.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 265)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677324 sequences (100000278 bp) in 1.669 CPU sec, 1.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288268, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.65, 102.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677800 reads in 314.345 CPU sec, 10.119 real sec
sorting block 40, #reads = 1082575 mem = 536879632 peak = 895762432
[M::mem_process_seqs] Processed 677404 reads in 126.854 CPU sec, 4.129 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288514, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.40, 104.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1082831 mem = 536879133 peak = 895762432
[M::process] read 677062 sequences (100000191 bp) in 2.522 CPU sec, 2.930 real sec...
[M::process] read 677102 sequences (100000289 bp) in 1.677 CPU sec, 1.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288859, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (222.05, 104.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677412 reads in 213.242 CPU sec, 6.923 real sec
[M::mem_process_seqs] Processed 677324 reads in 121.208 CPU sec, 3.928 real sec
sorting block 42, #reads = 1082527 mem = 536878174 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288356, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.24, 107.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676912 sequences (100000063 bp) in 2.527 CPU sec, 2.817 real sec...
[M::process] read 676824 sequences (100000162 bp) in 1.372 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288588, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.76, 110.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677062 reads in 216.884 CPU sec, 7.010 real sec
[M::mem_process_seqs] Processed 677102 reads in 122.767 CPU sec, 3.963 real sec
sorting block 43, #reads = 1082607 mem = 536877514 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288289, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.65, 111.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676682 sequences (100000001 bp) in 2.346 CPU sec, 2.686 real sec...
[M::process] read 676618 sequences (100000254 bp) in 1.712 CPU sec, 1.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287421, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.29, 112.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676912 reads in 215.182 CPU sec, 6.988 real sec
[M::mem_process_seqs] Processed 676824 reads in 121.813 CPU sec, 3.975 real sec
sorting block 44, #reads = 1082357 mem = 536880414 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288053, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.48, 114.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1082248 mem = 536878762 peak = 895762432
[M::process] read 677542 sequences (100000000 bp) in 2.607 CPU sec, 2.805 real sec...
[M::process] read 677660 sequences (100000206 bp) in 1.701 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287635, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.02, 115.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676682 reads in 213.316 CPU sec, 6.868 real sec
[M::mem_process_seqs] Processed 676618 reads in 121.069 CPU sec, 3.917 real sec
sorting block 46, #reads = 1082587 mem = 536877965 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288847, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (217.34, 98.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677574 sequences (100000107 bp) in 2.377 CPU sec, 2.618 real sec...
[M::process] read 677348 sequences (100000214 bp) in 1.758 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288113, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (215.91, 95.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677542 reads in 209.915 CPU sec, 6.764 real sec
[M::mem_process_seqs] Processed 677660 reads in 119.380 CPU sec, 3.852 real sec
sorting block 47, #reads = 1082559 mem = 536879499 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288269, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.16, 101.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677282 sequences (100000171 bp) in 2.789 CPU sec, 3.080 real sec...
[M::process] read 677288 sequences (100000018 bp) in 1.553 CPU sec, 1.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288280, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.73, 103.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677574 reads in 213.525 CPU sec, 6.867 real sec
[M::mem_process_seqs] Processed 677348 reads in 119.317 CPU sec, 3.822 real sec
sorting block 48, #reads = 1082585 mem = 536879363 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288190, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.32, 105.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676992 sequences (100000029 bp) in 2.682 CPU sec, 2.938 real sec...
[M::process] read 676988 sequences (100000088 bp) in 1.729 CPU sec, 1.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288087, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.21, 106.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677282 reads in 208.839 CPU sec, 6.802 real sec
[M::mem_process_seqs] Processed 677288 reads in 117.576 CPU sec, 3.898 real sec
sorting block 49, #reads = 1082560 mem = 536878114 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288195, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (224.89, 107.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1082353 mem = 536877421 peak = 895762432
[M::process] read 676846 sequences (100000198 bp) in 2.710 CPU sec, 2.977 real sec...
[M::process] read 676742 sequences (100000058 bp) in 1.679 CPU sec, 1.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288099, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.19, 110.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676992 reads in 212.726 CPU sec, 6.855 real sec
[M::mem_process_seqs] Processed 676988 reads in 119.369 CPU sec, 3.852 real sec
sorting block 51, #reads = 1082553 mem = 536877316 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287899, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.01, 111.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676580 sequences (100000162 bp) in 2.609 CPU sec, 3.013 real sec...
[M::process] read 676640 sequences (100000164 bp) in 1.693 CPU sec, 1.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287375, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.71, 112.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676846 reads in 217.932 CPU sec, 7.033 real sec
[M::mem_process_seqs] Processed 676742 reads in 123.803 CPU sec, 3.971 real sec
sorting block 52, #reads = 1082886 mem = 536879380 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288145, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.35, 115.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 79, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (155.69, 177.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677402 sequences (100000115 bp) in 2.807 CPU sec, 3.113 real sec...
[M::process] read 677710 sequences (100000205 bp) in 1.489 CPU sec, 1.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287799, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.23, 116.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 197, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718)
[M::mem_pestat] mean and std.dev: (163.08, 190.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676580 reads in 213.909 CPU sec, 6.919 real sec
[M::mem_process_seqs] Processed 676640 reads in 120.529 CPU sec, 3.924 real sec
sorting block 53, #reads = 1082199 mem = 536878807 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288460, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (222.78, 105.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 405, 613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1825)
[M::mem_pestat] mean and std.dev: (405.40, 452.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2431)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677622 sequences (100000291 bp) in 2.484 CPU sec, 2.767 real sec...
[M::process] read 677274 sequences (100000091 bp) in 1.840 CPU sec, 1.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288700, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (214.21, 93.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677402 reads in 206.917 CPU sec, 6.668 real sec
[M::mem_process_seqs] Processed 677710 reads in 120.951 CPU sec, 3.867 real sec
sorting block 54, #reads = 1082125 mem = 536877142 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288266, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.67, 99.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1082874 mem = 536878235 peak = 895762432
[M::process] read 677264 sequences (100000223 bp) in 4.238 CPU sec, 4.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287990, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.28, 108.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677622 reads in 215.854 CPU sec, 6.998 real sec
[M::mem_process_seqs] Processed 677274 reads in 124.853 CPU sec, 4.063 real sec
[M::process] read 677168 sequences (100000256 bp) in 2.146 CPU sec, 2.374 real sec...
sorting block 56, #reads = 1082708 mem = 536879678 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288235, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.06, 106.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 187, 187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (117.00, 82.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 712)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677016 sequences (100000161 bp) in 2.315 CPU sec, 2.587 real sec...
[M::process] read 676954 sequences (100000291 bp) in 1.861 CPU sec, 1.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287733, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.91, 107.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 89, 2350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6872)
[M::mem_pestat] mean and std.dev: (766.30, 1037.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9133)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677264 reads in 218.065 CPU sec, 7.037 real sec
[M::mem_process_seqs] Processed 677168 reads in 124.440 CPU sec, 4.014 real sec
sorting block 57, #reads = 1082423 mem = 536879220 peak = 895762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288256, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.30, 109.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676832 sequences (100000040 bp) in 2.937 CPU sec, 3.258 real sec...
[M::process] read 676732 sequences (100000208 bp) in 1.726 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287668, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.47, 110.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677016 reads in 210.260 CPU sec, 6.753 real sec
[M::mem_process_seqs] Processed 676954 reads in 117.980 CPU sec, 3.767 real sec
sorting block 58, #reads = 1082761 mem = 536881029 peak = 895762432
[M::process] read 676482 sequences (100000158 bp) in 1.783 CPU sec, 1.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287942, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.14, 114.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676420 sequences (100000183 bp) in 1.215 CPU sec, 1.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287512, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.72, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 441, 1172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3278)
[M::mem_pestat] mean and std.dev: (514.69, 430.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4331)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287929, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.67, 113.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 49, 116)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322)
[M::mem_pestat] mean and std.dev: (41.38, 42.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 425)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676832 reads in 292.438 CPU sec, 9.414 real sec
sorting block 59, #reads = 1082132 mem = 536880781 peak = 895762432
[M::mem_process_seqs] Processed 676482 reads in 203.085 CPU sec, 6.546 real sec
[M::mem_process_seqs] Processed 676732 reads in 211.254 CPU sec, 6.829 real sec
sorting block 60, #reads = 1081571 mem = 536879159 peak = 895762432
[M::process] read 563328 sequences (83266665 bp) in 2.216 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287143, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.00, 120.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 239390, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.11, 118.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676420 reads in 188.370 CPU sec, 6.151 real sec
[M::mem_process_seqs] Processed 563328 reads in 94.701 CPU sec, 3.080 real sec
sorting block 61, #reads = 1082052 mem = 536878150 peak = 895762432
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67585608
overall: 69252608000 mem 12697.277 user 819.191 sys 459.784 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L006\tPU:L006\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T21-D0003376_R1.fastq.gz 0003.T21-D0003376_R2.fastq.gz
[main] Real time: 459.814 sec; CPU: 13516.471 sec
sorting block 62, #reads = 1000715 mem = 496619468 peak = 895762432
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 2651053179
output reads: 68111250
bam_mem_sort: 63 calls 47.785 user 0.919 sys 49.400 real
bam_write: 63 calls 39.547 user 3.495 sys 71.978 real
execute: 1 calls 22.992 user 2.575 sys 487.803 real
merge_files: 1 calls 22.970 user 2.543 sys 27.064 real
parse_chunk: 1007 calls 48.168 user 14.421 sys 71.808 real
read_chunk: 2712 calls 33.518 user 14.368 sys 368.483 real
sort_block: 1 calls 24.541 user 13.025 sys 353.519 real
write_chunk: 1703 calls 22.398 user 2.343 sys 25.985 real
overall: 2053447680 mem 797.197 user 69.152 sys 487.821 real
ls: cannot access '*.cram': No such file or directory