Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c7/bd1e6077ffc96d6cef91c26dc5bedf/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/b8145cebcda724984d019f97962525/T21-D0003376/0001.T21-D0003376_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c7/bd1e6077ffc96d6cef91c26dc5bedf/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/b8145cebcda724984d019f97962525/T21-D0003376/0001.T21-D0003376_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1872.712 CPU sec, 68.716 real sec
[M::process] read 677536 sequences (100000230 bp) in 1.288 CPU sec, 1.293 real sec...
[M::process] read 677476 sequences (100000191 bp) in 1.620 CPU sec, 1.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289031, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (219.03, 98.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 113, 769)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2291)
[M::mem_pestat] mean and std.dev: (200.70, 277.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3052)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677386 sequences (100000215 bp) in 1.522 CPU sec, 1.530 real sec...
[M::process] read 676966 sequences (100000284 bp) in 1.510 CPU sec, 1.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288764, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (220.92, 101.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677536 reads in 189.127 CPU sec, 5.996 real sec
[M::process] read 677010 sequences (100000295 bp) in 1.842 CPU sec, 1.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288859, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (223.09, 103.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677386 reads in 101.282 CPU sec, 3.232 real sec
sorting block 0, #reads = 1082820 mem = 536878710 peak = 671236096
[M::mem_process_seqs] Processed 677476 reads in 228.483 CPU sec, 7.318 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288495, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (225.08, 105.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 187, 1705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5073)
[M::mem_pestat] mean and std.dev: (566.30, 748.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6757)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676768 sequences (100000161 bp) in 2.233 CPU sec, 2.250 real sec...
[M::process] read 676664 sequences (100000261 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288301, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.62, 109.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676966 reads in 194.325 CPU sec, 6.202 real sec
sorting block 1, #reads = 1082871 mem = 536877619 peak = 982347776
[M::mem_process_seqs] Processed 677010 reads in 105.219 CPU sec, 3.364 real sec
sorting block 2, #reads = 1082587 mem = 536880702 peak = 982347776
[M::process] read 676446 sequences (100000083 bp) in 2.411 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288471, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.26, 110.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676440 sequences (100000203 bp) in 1.449 CPU sec, 1.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288177, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.68, 113.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676768 reads in 189.316 CPU sec, 6.025 real sec
[M::mem_process_seqs] Processed 676664 reads in 105.764 CPU sec, 3.381 real sec
sorting block 3, #reads = 1082394 mem = 536879855 peak = 982347776
[M::process] read 676404 sequences (100000031 bp) in 2.107 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287598, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.18, 117.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676292 sequences (100000203 bp) in 1.390 CPU sec, 1.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287892, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.44, 117.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287787, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.00, 117.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676446 reads in 280.323 CPU sec, 8.978 real sec
[M::mem_process_seqs] Processed 676440 reads in 194.617 CPU sec, 6.256 real sec
[M::mem_process_seqs] Processed 676404 reads in 204.001 CPU sec, 6.576 real sec
sorting block 4, #reads = 1082236 mem = 536880964 peak = 982347776
[M::process] read 676056 sequences (100000173 bp) in 2.098 CPU sec, 2.179 real sec...
sorting block 5, #reads = 1082345 mem = 536877151 peak = 982347776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287791, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.09, 118.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676590 sequences (100000243 bp) in 2.122 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287674, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.87, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676292 reads in 192.205 CPU sec, 6.086 real sec
[M::process] read 677630 sequences (100000025 bp) in 1.790 CPU sec, 1.787 real sec...
[M::mem_process_seqs] Processed 676056 reads in 107.117 CPU sec, 3.370 real sec
sorting block 6, #reads = 1082161 mem = 536879147 peak = 982347776
[M::process] read 677490 sequences (100000015 bp) in 2.462 CPU sec, 2.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288143, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.76, 113.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677118 sequences (100000247 bp) in 2.020 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288974, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (219.35, 99.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289043, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.80, 102.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676590 reads in 264.296 CPU sec, 8.419 real sec
[M::mem_process_seqs] Processed 677630 reads in 182.046 CPU sec, 5.821 real sec
[M::mem_process_seqs] Processed 677490 reads in 191.115 CPU sec, 6.131 real sec
sorting block 7, #reads = 1081457 mem = 536880971 peak = 982347776
sorting block 8, #reads = 1082347 mem = 536877857 peak = 1002307584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288444, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.70, 105.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677118 reads in 100.263 CPU sec, 3.296 real sec
[M::process] read 677116 sequences (100000254 bp) in 3.130 CPU sec, 3.319 real sec...
sorting block 9, #reads = 1082480 mem = 536878680 peak = 1002307584
[M::process] read 676922 sequences (100000128 bp) in 2.036 CPU sec, 2.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288389, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.59, 107.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676812 sequences (100000055 bp) in 1.520 CPU sec, 1.521 real sec...
[M::process] read 676836 sequences (100000065 bp) in 1.490 CPU sec, 1.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288603, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.11, 109.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677116 reads in 183.393 CPU sec, 5.778 real sec
[M::mem_process_seqs] Processed 676922 reads in 103.601 CPU sec, 3.267 real sec
sorting block 10, #reads = 1082529 mem = 536878727 peak = 1002307584
[M::process] read 676442 sequences (100000047 bp) in 2.241 CPU sec, 2.305 real sec...
[M::process] read 676454 sequences (100000191 bp) in 1.600 CPU sec, 1.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287739, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.48, 115.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676836 reads in 96.378 CPU sec, 3.060 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288725, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.60, 111.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676404 sequences (100000264 bp) in 1.542 CPU sec, 1.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288136, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.58, 116.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676812 reads in 279.517 CPU sec, 8.893 real sec
sorting block 11, #reads = 1081859 mem = 536878708 peak = 1002307584
[M::process] read 676290 sequences (100000281 bp) in 1.865 CPU sec, 1.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288366, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.97, 117.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676454 reads in 97.154 CPU sec, 3.081 real sec
sorting block 12, #reads = 1082182 mem = 536880108 peak = 1018421248
[M::mem_process_seqs] Processed 676442 reads in 322.143 CPU sec, 10.256 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287900, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.51, 118.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 26, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (93.15, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676240 sequences (100000244 bp) in 2.583 CPU sec, 2.625 real sec...
[M::process] read 677180 sequences (100000080 bp) in 1.950 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287765, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.45, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 189, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 468)
[M::mem_pestat] mean and std.dev: (221.71, 89.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676404 reads in 195.589 CPU sec, 6.187 real sec
[M::mem_process_seqs] Processed 676290 reads in 106.645 CPU sec, 3.369 real sec
sorting block 13, #reads = 1082299 mem = 536877660 peak = 1018421248
sorting block 14, #reads = 1081904 mem = 536877006 peak = 1063522304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288225, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.51, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677358 sequences (100000063 bp) in 2.517 CPU sec, 2.568 real sec...
[M::process] read 677380 sequences (100000213 bp) in 2.010 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288350, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.48, 106.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676240 reads in 185.232 CPU sec, 5.867 real sec
[M::mem_process_seqs] Processed 677180 reads in 104.547 CPU sec, 3.310 real sec
sorting block 15, #reads = 1081748 mem = 536879022 peak = 1063522304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288618, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.52, 100.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (330, 330, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (184, 549)
[M::mem_pestat] mean and std.dev: (350.86, 32.98)
[M::mem_pestat] low and high boundaries for proper pairs: (111, 622)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677198 sequences (100000214 bp) in 2.734 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288619, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.33, 102.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676904 sequences (100000001 bp) in 2.100 CPU sec, 2.101 real sec...
[M::mem_process_seqs] Processed 677358 reads in 188.274 CPU sec, 5.974 real sec
[M::mem_process_seqs] Processed 677380 reads in 105.915 CPU sec, 3.332 real sec
sorting block 16, #reads = 1082627 mem = 536878700 peak = 1063522304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288240, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.18, 104.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676802 sequences (100000137 bp) in 2.586 CPU sec, 2.617 real sec...
[M::process] read 676706 sequences (100000077 bp) in 2.030 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288224, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.52, 109.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677198 reads in 185.572 CPU sec, 5.877 real sec
[M::mem_process_seqs] Processed 676904 reads in 105.168 CPU sec, 3.333 real sec
sorting block 17, #reads = 1082472 mem = 536877023 peak = 1063522304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287846, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.22, 110.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676438 sequences (100000272 bp) in 2.752 CPU sec, 2.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288181, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.78, 113.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676424 sequences (100000225 bp) in 2.090 CPU sec, 2.091 real sec...
[M::mem_process_seqs] Processed 676802 reads in 189.404 CPU sec, 6.002 real sec
[M::mem_process_seqs] Processed 676706 reads in 108.063 CPU sec, 3.393 real sec
sorting block 18, #reads = 1082414 mem = 536880295 peak = 1074053120
sorting block 19, #reads = 1081954 mem = 536878857 peak = 1082912768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287911, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.70, 115.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676412 sequences (100000100 bp) in 2.840 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287673, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.22, 117.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676362 sequences (100000162 bp) in 2.050 CPU sec, 2.058 real sec...
[M::mem_process_seqs] Processed 676438 reads in 193.451 CPU sec, 6.139 real sec
[M::mem_process_seqs] Processed 676424 reads in 109.893 CPU sec, 3.457 real sec
sorting block 20, #reads = 1082229 mem = 536879148 peak = 1082912768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288256, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.95, 117.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676290 sequences (100000212 bp) in 2.811 CPU sec, 2.907 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288015, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.55, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676306 sequences (100000242 bp) in 2.290 CPU sec, 2.289 real sec...
[M::mem_process_seqs] Processed 676412 reads in 190.853 CPU sec, 6.043 real sec
[M::mem_process_seqs] Processed 676362 reads in 108.359 CPU sec, 3.412 real sec
sorting block 21, #reads = 1081664 mem = 536881003 peak = 1082912768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288096, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.29, 120.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677670 sequences (100000111 bp) in 2.863 CPU sec, 2.920 real sec...
[M::process] read 677386 sequences (100000017 bp) in 2.077 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288135, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.85, 119.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676290 reads in 189.500 CPU sec, 6.005 real sec
[M::mem_process_seqs] Processed 676306 reads in 106.890 CPU sec, 3.369 real sec
sorting block 22, #reads = 1081994 mem = 536879273 peak = 1082912768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288631, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (217.03, 95.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677232 sequences (100000091 bp) in 2.759 CPU sec, 2.867 real sec...
[M::process] read 677080 sequences (100000002 bp) in 2.080 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288579, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (220.59, 100.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 50, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577)
[M::mem_pestat] mean and std.dev: (170.50, 245.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2094)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677670 reads in 186.584 CPU sec, 5.910 real sec
sorting block 23, #reads = 1082112 mem = 536877414 peak = 1082912768
[M::mem_process_seqs] Processed 677386 reads in 105.815 CPU sec, 3.333 real sec
sorting block 24, #reads = 1082543 mem = 536880636 peak = 1090068480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288242, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (223.06, 102.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676924 sequences (100000293 bp) in 2.789 CPU sec, 2.817 real sec...
[M::process] read 676680 sequences (100000036 bp) in 2.100 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287976, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.80, 104.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677232 reads in 191.694 CPU sec, 6.070 real sec
[M::mem_process_seqs] Processed 677080 reads in 107.221 CPU sec, 3.392 real sec
sorting block 25, #reads = 1082555 mem = 536879370 peak = 1090068480
[M::process] read 676574 sequences (100000263 bp) in 2.504 CPU sec, 2.527 real sec...
[M::process] read 676542 sequences (100000291 bp) in 2.150 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287943, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.87, 110.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676680 reads in 103.754 CPU sec, 3.265 real sec
sorting block 26, #reads = 1082190 mem = 536880058 peak = 1090068480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288202, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (227.94, 108.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288089, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.65, 112.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676460 sequences (100000020 bp) in 2.035 CPU sec, 2.038 real sec...
[M::mem_process_seqs] Processed 676924 reads in 296.728 CPU sec, 9.413 real sec
[M::mem_process_seqs] Processed 676574 reads in 120.889 CPU sec, 3.850 real sec
sorting block 27, #reads = 1082160 mem = 536879647 peak = 1090068480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287311, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.91, 115.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676394 sequences (100000213 bp) in 2.850 CPU sec, 3.058 real sec...
[M::process] read 676246 sequences (100000281 bp) in 1.990 CPU sec, 1.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287542, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.95, 116.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676542 reads in 195.944 CPU sec, 6.223 real sec
[M::mem_process_seqs] Processed 676460 reads in 111.221 CPU sec, 3.511 real sec
sorting block 28, #reads = 1082221 mem = 536879252 peak = 1090068480
[M::process] read 676204 sequences (100000006 bp) in 2.572 CPU sec, 2.650 real sec...
[M::process] read 676162 sequences (100000057 bp) in 1.620 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287815, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.71, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676246 reads in 100.233 CPU sec, 3.185 real sec
sorting block 29, #reads = 1081691 mem = 536878891 peak = 1122193408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287639, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.31, 117.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677848 sequences (100000216 bp) in 1.771 CPU sec, 1.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287715, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.15, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676394 reads in 295.251 CPU sec, 9.378 real sec
[M::mem_process_seqs] Processed 676204 reads in 209.161 CPU sec, 6.651 real sec
sorting block 30, #reads = 1082043 mem = 536877767 peak = 1122193408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288317, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (240.41, 121.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677670 sequences (100000134 bp) in 2.853 CPU sec, 2.895 real sec...
[M::process] read 677348 sequences (100000270 bp) in 1.650 CPU sec, 1.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288742, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (208.48, 84.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676162 reads in 190.768 CPU sec, 6.059 real sec
[M::mem_process_seqs] Processed 677848 reads in 106.097 CPU sec, 3.360 real sec
sorting block 31, #reads = 1082193 mem = 536878272 peak = 1122193408
[M::process] read 677102 sequences (100000271 bp) in 2.098 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288703, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (213.48, 91.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 17, 170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (37.30, 50.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 647)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677072 sequences (100000241 bp) in 1.620 CPU sec, 1.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288136, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.29, 105.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288420, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (222.22, 102.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677670 reads in 278.554 CPU sec, 8.901 real sec
[M::mem_process_seqs] Processed 677102 reads in 194.807 CPU sec, 6.253 real sec
sorting block 32, #reads = 1082505 mem = 536878723 peak = 1159774208
[M::mem_process_seqs] Processed 677348 reads in 204.223 CPU sec, 6.574 real sec
sorting block 33, #reads = 1082439 mem = 536876911 peak = 1159774208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288131, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.34, 107.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677072 reads in 106.876 CPU sec, 3.535 real sec
[M::process] read 676908 sequences (100000066 bp) in 3.555 CPU sec, 3.715 real sec...
sorting block 34, #reads = 1082337 mem = 536877079 peak = 1159774208
[M::process] read 676874 sequences (100000284 bp) in 2.806 CPU sec, 2.850 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287742, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (226.96, 107.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676806 sequences (100000275 bp) in 2.200 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287691, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.46, 109.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 100, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (187.77, 227.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676908 reads in 195.514 CPU sec, 6.156 real sec
[M::mem_process_seqs] Processed 676874 reads in 112.220 CPU sec, 3.517 real sec
[M::process] read 676794 sequences (100000196 bp) in 2.523 CPU sec, 2.553 real sec...
sorting block 35, #reads = 1082204 mem = 536883139 peak = 1159774208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287891, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.87, 112.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676688 sequences (100000248 bp) in 2.623 CPU sec, 2.697 real sec...
[M::process] read 676642 sequences (100000145 bp) in 1.650 CPU sec, 1.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287893, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.53, 112.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676806 reads in 198.659 CPU sec, 6.305 real sec
[M::mem_process_seqs] Processed 676794 reads in 111.135 CPU sec, 3.515 real sec
sorting block 36, #reads = 1082452 mem = 536879667 peak = 1159774208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288370, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.31, 113.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 78, 125)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (42.33, 47.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676586 sequences (100000268 bp) in 2.594 CPU sec, 2.656 real sec...
[M::process] read 677304 sequences (100000173 bp) in 2.220 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288210, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.09, 114.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676688 reads in 194.778 CPU sec, 6.173 real sec
[M::mem_process_seqs] Processed 676642 reads in 108.633 CPU sec, 3.426 real sec
sorting block 37, #reads = 1082461 mem = 536879564 peak = 1159774208
sorting block 38, #reads = 1082874 mem = 536882087 peak = 1159774208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287918, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.57, 114.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677810 sequences (100000032 bp) in 2.564 CPU sec, 2.604 real sec...
[M::process] read 677702 sequences (100000003 bp) in 1.879 CPU sec, 1.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288477, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (220.83, 102.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676586 reads in 195.558 CPU sec, 6.218 real sec
[M::mem_process_seqs] Processed 677304 reads in 109.825 CPU sec, 3.488 real sec
sorting block 39, #reads = 1081688 mem = 536877474 peak = 1159774208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288856, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (206.57, 82.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 58, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827)
[M::mem_pestat] mean and std.dev: (252.14, 299.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2430)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677462 sequences (100000294 bp) in 2.729 CPU sec, 2.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288976, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (213.86, 93.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677184 sequences (100000007 bp) in 2.190 CPU sec, 2.188 real sec...
[M::mem_process_seqs] Processed 677810 reads in 189.262 CPU sec, 5.991 real sec
[M::mem_process_seqs] Processed 677702 reads in 106.847 CPU sec, 3.358 real sec
sorting block 40, #reads = 1082543 mem = 536879524 peak = 1159774208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288593, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.09, 102.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677060 sequences (100000173 bp) in 2.506 CPU sec, 2.565 real sec...
[M::process] read 677204 sequences (100000085 bp) in 1.730 CPU sec, 1.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288406, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (223.15, 105.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 211, 640)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1888)
[M::mem_pestat] mean and std.dev: (267.11, 259.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2512)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677462 reads in 189.578 CPU sec, 6.031 real sec
[M::mem_process_seqs] Processed 677184 reads in 106.602 CPU sec, 3.384 real sec
sorting block 41, #reads = 1083176 mem = 536879408 peak = 1166999552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288649, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (224.87, 106.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676910 sequences (100000273 bp) in 2.713 CPU sec, 2.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288018, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (224.85, 107.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677060 reads in 190.294 CPU sec, 6.038 real sec
[M::process] read 676846 sequences (100000106 bp) in 2.318 CPU sec, 2.314 real sec...
sorting block 42, #reads = 1082461 mem = 536882275 peak = 1166999552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287818, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.22, 109.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 232, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913)
[M::mem_pestat] mean and std.dev: (377.36, 385.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2513)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676804 sequences (100000011 bp) in 2.707 CPU sec, 2.709 real sec...
[M::mem_process_seqs] Processed 676910 reads in 107.218 CPU sec, 3.379 real sec
sorting block 43, #reads = 1082423 mem = 536878809 peak = 1166999552
[M::mem_process_seqs] Processed 677204 reads in 246.250 CPU sec, 7.774 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288095, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.49, 110.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676758 sequences (100000212 bp) in 2.214 CPU sec, 2.258 real sec...
[M::process] read 676660 sequences (100000277 bp) in 1.630 CPU sec, 1.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288135, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.70, 112.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676846 reads in 209.551 CPU sec, 6.654 real sec
[M::mem_process_seqs] Processed 676804 reads in 111.258 CPU sec, 3.533 real sec
sorting block 44, #reads = 1082221 mem = 536878455 peak = 1166999552
sorting block 45, #reads = 1081978 mem = 536881229 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287799, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.88, 113.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 34, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350)
[M::mem_pestat] mean and std.dev: (294.15, 405.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3130)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677112 sequences (100000225 bp) in 2.760 CPU sec, 2.818 real sec...
[M::process] read 678002 sequences (100000206 bp) in 2.200 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288364, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.42, 114.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676758 reads in 190.425 CPU sec, 6.032 real sec
[M::mem_process_seqs] Processed 676660 reads in 105.874 CPU sec, 3.348 real sec
sorting block 46, #reads = 1082057 mem = 536879840 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288861, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (223.49, 106.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677670 sequences (100000113 bp) in 2.401 CPU sec, 2.438 real sec...
[M::process] read 677512 sequences (100000141 bp) in 1.700 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289008, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 175, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (201.41, 75.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677112 reads in 186.797 CPU sec, 5.940 real sec
[M::mem_process_seqs] Processed 678002 reads in 106.484 CPU sec, 3.379 real sec
sorting block 47, #reads = 1082977 mem = 536879150 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288600, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (209.37, 86.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677458 sequences (100000106 bp) in 3.022 CPU sec, 3.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288454, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.62, 100.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677270 sequences (100000115 bp) in 2.230 CPU sec, 2.228 real sec...
[M::mem_process_seqs] Processed 677670 reads in 191.215 CPU sec, 6.056 real sec
[M::mem_process_seqs] Processed 677512 reads in 107.171 CPU sec, 3.368 real sec
sorting block 48, #reads = 1083299 mem = 536879871 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288545, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (221.71, 103.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 179, 2021)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6027)
[M::mem_pestat] mean and std.dev: (1166.30, 1748.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8159)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677064 sequences (100000079 bp) in 2.235 CPU sec, 2.290 real sec...
[M::process] read 677040 sequences (100000039 bp) in 2.130 CPU sec, 2.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288007, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (224.46, 106.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 742)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (323, 441, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (369.91, 200.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1515)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677458 reads in 193.550 CPU sec, 6.160 real sec
[M::mem_process_seqs] Processed 677270 reads in 109.989 CPU sec, 3.484 real sec
sorting block 49, #reads = 1082584 mem = 536878852 peak = 1212805120
sorting block 50, #reads = 1082738 mem = 536877814 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288591, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.77, 108.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676994 sequences (100000014 bp) in 2.984 CPU sec, 3.012 real sec...
[M::process] read 676940 sequences (100000031 bp) in 2.230 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287748, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.17, 108.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677064 reads in 197.108 CPU sec, 6.239 real sec
[M::mem_process_seqs] Processed 677040 reads in 111.900 CPU sec, 3.537 real sec
sorting block 51, #reads = 1082703 mem = 536877698 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288220, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.78, 110.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676710 sequences (100000138 bp) in 2.716 CPU sec, 2.761 real sec...
[M::process] read 676698 sequences (100000073 bp) in 2.200 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287953, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.40, 112.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676994 reads in 193.876 CPU sec, 6.146 real sec
[M::mem_process_seqs] Processed 676940 reads in 109.454 CPU sec, 3.448 real sec
sorting block 52, #reads = 1082798 mem = 536876196 peak = 1212805120
[M::process] read 677022 sequences (100000201 bp) in 2.796 CPU sec, 2.832 real sec...
[M::process] read 677892 sequences (100000059 bp) in 2.199 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288021, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (228.99, 111.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676698 reads in 101.167 CPU sec, 3.188 real sec
sorting block 53, #reads = 1082602 mem = 536880467 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287926, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.69, 112.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677760 sequences (100000237 bp) in 1.676 CPU sec, 1.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287952, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (223.92, 106.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (29, 66, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325)
[M::mem_pestat] mean and std.dev: (332.60, 419.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2011)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676710 reads in 297.332 CPU sec, 9.439 real sec
[M::mem_process_seqs] Processed 677022 reads in 120.282 CPU sec, 3.838 real sec
sorting block 54, #reads = 1082016 mem = 536878470 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288905, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 175, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (202.62, 76.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677506 sequences (100000192 bp) in 3.095 CPU sec, 3.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288773, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (211.60, 89.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677892 reads in 191.070 CPU sec, 6.083 real sec
[M::mem_process_seqs] Processed 677760 reads in 111.307 CPU sec, 3.496 real sec
[M::process] read 677348 sequences (100000015 bp) in 2.544 CPU sec, 2.570 real sec...
sorting block 55, #reads = 1082650 mem = 536877181 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288226, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 176, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (216.40, 96.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677290 sequences (100000223 bp) in 2.927 CPU sec, 3.011 real sec...
[M::process] read 677140 sequences (100000063 bp) in 1.680 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288199, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (218.50, 99.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677506 reads in 198.535 CPU sec, 6.300 real sec
[M::mem_process_seqs] Processed 677348 reads in 111.814 CPU sec, 3.532 real sec
sorting block 56, #reads = 1083063 mem = 536881774 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287645, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.20, 103.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677072 sequences (100000009 bp) in 2.584 CPU sec, 2.656 real sec...
[M::process] read 676934 sequences (100000193 bp) in 1.760 CPU sec, 1.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287710, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.70, 107.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 280, 2985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8767)
[M::mem_pestat] mean and std.dev: (953.91, 1254.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11658)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677290 reads in 202.742 CPU sec, 6.457 real sec
sorting block 57, #reads = 1082626 mem = 536879005 peak = 1212805120
[M::mem_process_seqs] Processed 677140 reads in 112.256 CPU sec, 3.558 real sec
sorting block 58, #reads = 1082498 mem = 536879936 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288368, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.36, 108.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676752 sequences (100000085 bp) in 2.622 CPU sec, 2.671 real sec...
[M::process] read 676728 sequences (100000102 bp) in 1.870 CPU sec, 1.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288015, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.81, 110.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677072 reads in 200.045 CPU sec, 6.358 real sec
[M::mem_process_seqs] Processed 676934 reads in 110.422 CPU sec, 3.506 real sec
sorting block 59, #reads = 1083016 mem = 536880194 peak = 1212805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288162, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.97, 111.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676594 sequences (100000250 bp) in 2.512 CPU sec, 2.589 real sec...
[M::process] read 676632 sequences (100000008 bp) in 1.844 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287726, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.72, 114.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676752 reads in 201.349 CPU sec, 6.422 real sec
[M::mem_process_seqs] Processed 676728 reads in 113.507 CPU sec, 3.593 real sec
sorting block 60, #reads = 1082934 mem = 536880037 peak = 1212805120
[M::process] read 34562 sequences (5106079 bp) in 0.506 CPU sec, 0.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288263, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.52, 115.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 172, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (157.50, 103.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 14652, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.12, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288090, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.59, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676594 reads in 198.843 CPU sec, 6.440 real sec
[M::mem_process_seqs] Processed 34562 reads in 104.537 CPU sec, 3.373 real sec
[M::mem_process_seqs] Processed 676632 reads in 113.683 CPU sec, 3.683 real sec
sorting block 61, #reads = 1082506 mem = 536878286 peak = 1243049984
sorting block 62, #reads = 1082580 mem = 536878866 peak = 1243049984
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67728846
overall: 69736988672 mem 11644.151 user 286.682 sys 399.684 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L008\tPU:L008\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T21-D0003376_R1.fastq.gz 0001.T21-D0003376_R2.fastq.gz
[main] Real time: 399.731 sec; CPU: 11930.849 sec
sorting block 63, #reads = 65986 mem = 32718309 peak = 1243049984
uniq header count 1
merging 64 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: bfebfbff 7ffefbff
  extended: d39ff7eb 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 2650227617
output reads: 68256676
bam_mem_sort: 64 calls 65.214 user 0.984 sys 66.273 real
bam_write: 64 calls 19.117 user 0.780 sys 19.775 real
execute: 1 calls 21.188 user 0.877 sys 423.533 real
merge_files: 1 calls 21.156 user 0.835 sys 23.729 real
parse_chunk: 1009 calls 83.680 user 15.999 sys 100.659 real
read_chunk: 2718 calls 32.843 user 21.793 sys 338.216 real
sort_block: 1 calls 21.920 user 21.302 sys 323.756 real
write_chunk: 1707 calls 20.507 user 0.603 sys 22.602 real
overall: 2557325312 mem 1008.552 user 52.151 sys 423.557 real
ls: cannot access '*.cram': No such file or directory