Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1c/e8841f10be58310658995c33a27bc4/T18-D0003255/0001.T18-D0003255_R1.fastq.gz
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/d923ffcb99d250cfb20a33f661f911/.command.sh
Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1c/e8841f10be58310658995c33a27bc4/T18-D0003255/0001.T18-D0003255_R2.fastq.gz
Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e8/d923ffcb99d250cfb20a33f661f911/.command.run
==> STAGING COMPLETE (7 inputs)
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1928.640 CPU sec, 248.970 real sec
[M::process] read 673288 sequences (100000072 bp) in 0.868 CPU sec, 0.874 real sec...
[M::process] read 673280 sequences (100000012 bp) in 1.240 CPU sec, 1.238 real sec...
[M::process] read 673144 sequences (100000182 bp) in 1.319 CPU sec, 1.322 real sec...
[M::process] read 673094 sequences (100000216 bp) in 1.210 CPU sec, 1.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285775, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (262.72, 130.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285848, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.20, 136.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285888, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.81, 133.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673288 reads in 357.506 CPU sec, 11.519 real sec
[M::mem_process_seqs] Processed 673144 reads in 216.351 CPU sec, 7.057 real sec
[M::mem_process_seqs] Processed 673280 reads in 230.474 CPU sec, 7.565 real sec
sorting block 0, #reads = 1079842 mem = 536880596 peak = 598933504
[M::process] read 673112 sequences (100000148 bp) in 2.041 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285260, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (270.60, 137.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 86, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218)
[M::mem_pestat] mean and std.dev: (76.30, 75.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 377)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672948 sequences (100000296 bp) in 1.394 CPU sec, 1.504 real sec...
[M::process] read 672792 sequences (100000093 bp) in 1.040 CPU sec, 1.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285151, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.74, 140.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 245, 1030)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998)
[M::mem_pestat] mean and std.dev: (514.40, 525.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3982)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673094 reads in 198.992 CPU sec, 6.416 real sec
sorting block 1, #reads = 1079842 mem = 536879702 peak = 753074176
[M::mem_process_seqs] Processed 673112 reads in 116.471 CPU sec, 3.763 real sec
sorting block 2, #reads = 1079582 mem = 536877189 peak = 838467584
[M::process] read 672802 sequences (100000270 bp) in 2.091 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285072, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.44, 141.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672690 sequences (100000179 bp) in 1.050 CPU sec, 1.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284556, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.70, 145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 114, 1208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3574)
[M::mem_pestat] mean and std.dev: (414.65, 516.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4757)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284363, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (276.03, 141.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672948 reads in 276.641 CPU sec, 8.894 real sec
[M::mem_process_seqs] Processed 672802 reads in 195.422 CPU sec, 6.328 real sec
[M::mem_process_seqs] Processed 672792 reads in 204.826 CPU sec, 6.670 real sec
sorting block 3, #reads = 1079487 mem = 536878716 peak = 838467584
[M::process] read 672644 sequences (100000290 bp) in 1.755 CPU sec, 1.964 real sec...
sorting block 4, #reads = 1079205 mem = 536880750 peak = 838467584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284753, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (279.99, 143.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672580 sequences (100000018 bp) in 1.348 CPU sec, 1.431 real sec...
[M::process] read 672788 sequences (100000272 bp) in 1.020 CPU sec, 1.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284767, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (284.56, 148.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672690 reads in 192.977 CPU sec, 6.205 real sec
[M::mem_process_seqs] Processed 672644 reads in 108.899 CPU sec, 3.517 real sec
sorting block 5, #reads = 1079759 mem = 536879904 peak = 838467584
[M::process] read 672910 sequences (100000240 bp) in 1.776 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284502, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (285.16, 149.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 362, 1825)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5209)
[M::mem_pestat] mean and std.dev: (790.08, 748.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6901)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672946 sequences (100000086 bp) in 1.040 CPU sec, 1.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284871, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.75, 148.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 128, 1638)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4896)
[M::mem_pestat] mean and std.dev: (611.40, 867.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6525)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672580 reads in 190.632 CPU sec, 6.118 real sec
[M::mem_process_seqs] Processed 672788 reads in 109.758 CPU sec, 3.584 real sec
sorting block 6, #reads = 1079119 mem = 536879651 peak = 838467584
[M::process] read 672996 sequences (100000289 bp) in 1.470 CPU sec, 1.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284450, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.71, 143.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 25, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1462)
[M::mem_pestat] mean and std.dev: (78.78, 152.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1945)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672780 sequences (100000075 bp) in 1.090 CPU sec, 1.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284965, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.42, 144.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285085, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.29, 144.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 318, 698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1936)
[M::mem_pestat] mean and std.dev: (320.31, 280.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2555)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672910 reads in 274.085 CPU sec, 8.812 real sec
[M::mem_process_seqs] Processed 672996 reads in 189.884 CPU sec, 6.175 real sec
[M::mem_process_seqs] Processed 672946 reads in 197.875 CPU sec, 6.462 real sec
sorting block 7, #reads = 1079422 mem = 536878900 peak = 838467584
sorting block 8, #reads = 1079803 mem = 536878266 peak = 838467584
[M::process] read 672962 sequences (100000033 bp) in 2.340 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284738, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.03, 146.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673270 sequences (100000225 bp) in 1.349 CPU sec, 1.415 real sec...
[M::process] read 673118 sequences (100000296 bp) in 1.080 CPU sec, 1.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285471, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.53, 141.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 114, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334)
[M::mem_pestat] mean and std.dev: (117.80, 74.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 417)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672780 reads in 181.621 CPU sec, 5.827 real sec
[M::mem_process_seqs] Processed 672962 reads in 100.844 CPU sec, 3.229 real sec
sorting block 9, #reads = 1079086 mem = 536879730 peak = 838467584
[M::process] read 673094 sequences (100000120 bp) in 1.540 CPU sec, 1.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286089, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (266.40, 133.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 86, 104)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262)
[M::mem_pestat] mean and std.dev: (62.43, 51.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 341)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672980 sequences (100000109 bp) in 1.020 CPU sec, 1.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285197, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (270.03, 136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285685, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.61, 140.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673270 reads in 257.458 CPU sec, 8.276 real sec
[M::mem_process_seqs] Processed 673118 reads in 182.682 CPU sec, 5.925 real sec
sorting block 10, #reads = 1079709 mem = 536877169 peak = 838467584
[M::mem_process_seqs] Processed 673094 reads in 191.646 CPU sec, 6.248 real sec
sorting block 11, #reads = 1079710 mem = 536880075 peak = 876122112
[M::process] read 673052 sequences (100000260 bp) in 1.701 CPU sec, 1.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284999, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.79, 141.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672922 sequences (100000170 bp) in 1.543 CPU sec, 1.640 real sec...
[M::process] read 672816 sequences (100000139 bp) in 1.060 CPU sec, 1.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285114, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.90, 142.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672980 reads in 185.148 CPU sec, 5.935 real sec
[M::mem_process_seqs] Processed 673052 reads in 104.473 CPU sec, 3.368 real sec
sorting block 12, #reads = 1079767 mem = 536877893 peak = 876122112
[M::process] read 672744 sequences (100000117 bp) in 1.604 CPU sec, 1.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284920, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.93, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672764 sequences (100000071 bp) in 1.060 CPU sec, 1.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285139, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (282.07, 147.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284568, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.49, 149.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 174, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (170.00, 135.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672922 reads in 273.721 CPU sec, 8.811 real sec
sorting block 13, #reads = 1078906 mem = 536878170 peak = 876122112
[M::mem_process_seqs] Processed 672816 reads in 199.973 CPU sec, 6.504 real sec
[M::mem_process_seqs] Processed 672744 reads in 212.528 CPU sec, 6.956 real sec
sorting block 14, #reads = 1079600 mem = 536878928 peak = 876122112
[M::process] read 672586 sequences (100000041 bp) in 1.838 CPU sec, 2.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284179, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.72, 148.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672606 sequences (100000013 bp) in 1.552 CPU sec, 1.676 real sec...
[M::process] read 672928 sequences (100000274 bp) in 1.060 CPU sec, 1.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284359, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.73, 151.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 98, 6448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19296)
[M::mem_pestat] mean and std.dev: (2230.17, 2987.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25720)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672764 reads in 204.789 CPU sec, 6.590 real sec
sorting block 15, #reads = 1079557 mem = 536878460 peak = 876122112
[M::mem_process_seqs] Processed 672586 reads in 123.436 CPU sec, 4.005 real sec
[M::process] read 672908 sequences (100000278 bp) in 1.758 CPU sec, 1.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283930, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.57, 151.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (62, 774, 3295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9761)
[M::mem_pestat] mean and std.dev: (1319.10, 1562.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12994)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672808 sequences (100000154 bp) in 1.070 CPU sec, 1.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284607, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.55, 145.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 81, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (70.89, 66.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284557, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.64, 146.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672606 reads in 298.849 CPU sec, 9.631 real sec
sorting block 16, #reads = 1079482 mem = 536879359 peak = 876122112
[M::mem_process_seqs] Processed 672928 reads in 215.931 CPU sec, 7.031 real sec
[M::mem_process_seqs] Processed 672908 reads in 229.436 CPU sec, 7.515 real sec
sorting block 17, #reads = 1079155 mem = 536879685 peak = 876122112
[M::process] read 672756 sequences (100000092 bp) in 2.171 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285121, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.25, 146.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 64, 1890)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5576)
[M::mem_pestat] mean and std.dev: (675.23, 1010.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7419)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672750 sequences (100000103 bp) in 2.208 CPU sec, 2.488 real sec...
[M::process] read 673308 sequences (100000105 bp) in 1.040 CPU sec, 1.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284896, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.30, 148.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672808 reads in 203.578 CPU sec, 6.567 real sec
sorting block 18, #reads = 1079840 mem = 536878148 peak = 876122112
[M::mem_process_seqs] Processed 672756 reads in 123.756 CPU sec, 3.994 real sec
sorting block 19, #reads = 1079794 mem = 536878678 peak = 876122112
[M::process] read 673236 sequences (100000077 bp) in 1.749 CPU sec, 1.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285033, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (285.98, 149.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 24, 655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1947)
[M::mem_pestat] mean and std.dev: (170.70, 267.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2593)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673122 sequences (100000263 bp) in 1.050 CPU sec, 1.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285558, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (267.08, 133.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 86, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (88.44, 92.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285972, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.95, 135.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672750 reads in 283.371 CPU sec, 9.141 real sec
[M::mem_process_seqs] Processed 673236 reads in 204.514 CPU sec, 6.663 real sec
[M::mem_process_seqs] Processed 673308 reads in 219.156 CPU sec, 7.187 real sec
sorting block 20, #reads = 1079109 mem = 536879284 peak = 876122112
[M::process] read 673096 sequences (100000010 bp) in 1.937 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285410, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.58, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673000 sequences (100000290 bp) in 1.696 CPU sec, 1.834 real sec...
[M::process] read 673016 sequences (100000128 bp) in 1.260 CPU sec, 1.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285473, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (274.04, 140.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673122 reads in 206.511 CPU sec, 6.648 real sec
sorting block 21, #reads = 1079951 mem = 536878677 peak = 876122112
[M::mem_process_seqs] Processed 673096 reads in 122.428 CPU sec, 3.960 real sec
sorting block 22, #reads = 1080063 mem = 536880577 peak = 918908928
[M::process] read 672888 sequences (100000229 bp) in 2.355 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284623, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.18, 143.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 96, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (111.11, 108.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672694 sequences (100000245 bp) in 1.100 CPU sec, 1.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285398, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.72, 143.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284662, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.40, 147.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (79, 300, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138)
[M::mem_pestat] mean and std.dev: (206.30, 167.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1491)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673000 reads in 292.894 CPU sec, 9.448 real sec
[M::mem_process_seqs] Processed 673016 reads in 211.963 CPU sec, 6.883 real sec
[M::mem_process_seqs] Processed 672888 reads in 226.611 CPU sec, 7.419 real sec
sorting block 23, #reads = 1079863 mem = 536879325 peak = 918908928
[M::process] read 672732 sequences (100000136 bp) in 2.134 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284598, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 205, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.89, 147.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 479, 1135)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333)
[M::mem_pestat] mean and std.dev: (538.87, 509.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4432)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 24, #reads = 1079764 mem = 536880418 peak = 918908928
[M::process] read 672760 sequences (100000145 bp) in 2.647 CPU sec, 2.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284630, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (283.64, 147.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672656 sequences (100000154 bp) in 1.190 CPU sec, 1.191 real sec...
[M::mem_process_seqs] Processed 672694 reads in 219.163 CPU sec, 7.056 real sec
[M::mem_process_seqs] Processed 672732 reads in 129.945 CPU sec, 4.190 real sec
sorting block 25, #reads = 1079736 mem = 536879915 peak = 918908928
[M::process] read 672894 sequences (100000062 bp) in 1.754 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283664, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.45, 148.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672988 sequences (100000136 bp) in 1.150 CPU sec, 1.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284523, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.44, 150.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 512, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500)
[M::mem_pestat] mean and std.dev: (281.50, 260.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1994)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284356, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.28, 144.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 16, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2157)
[M::mem_pestat] mean and std.dev: (203.08, 305.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2875)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672760 reads in 303.842 CPU sec, 9.803 real sec
[M::mem_process_seqs] Processed 672656 reads in 217.633 CPU sec, 7.087 real sec
[M::mem_process_seqs] Processed 672894 reads in 232.793 CPU sec, 7.633 real sec
sorting block 26, #reads = 1079133 mem = 536881880 peak = 918908928
[M::process] read 672776 sequences (100000246 bp) in 1.937 CPU sec, 2.171 real sec...
sorting block 27, #reads = 1079361 mem = 536878316 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284421, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.91, 145.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 44, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (147.33, 265.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1679)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672722 sequences (100000169 bp) in 1.667 CPU sec, 1.813 real sec...
[M::process] read 672692 sequences (100000096 bp) in 1.170 CPU sec, 1.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284272, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.09, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (432, 1183, 1183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2685)
[M::mem_pestat] mean and std.dev: (851.91, 449.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3436)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672988 reads in 214.347 CPU sec, 6.901 real sec
[M::mem_process_seqs] Processed 672776 reads in 128.374 CPU sec, 4.160 real sec
sorting block 28, #reads = 1079678 mem = 536877413 peak = 918908928
[M::process] read 672832 sequences (100000155 bp) in 2.071 CPU sec, 2.259 real sec...
[M::process] read 673244 sequences (100000050 bp) in 1.200 CPU sec, 1.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284439, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 205, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (284.48, 149.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672692 reads in 109.864 CPU sec, 3.564 real sec
sorting block 29, #reads = 1079389 mem = 536879787 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284242, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.99, 147.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 92, 687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1887)
[M::mem_pestat] mean and std.dev: (354.53, 478.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2487)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673168 sequences (100000029 bp) in 1.100 CPU sec, 1.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285302, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (276.18, 142.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672722 reads in 311.055 CPU sec, 10.035 real sec
[M::mem_process_seqs] Processed 672832 reads in 229.812 CPU sec, 7.503 real sec
sorting block 30, #reads = 1079122 mem = 536879584 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285111, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.75, 132.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673102 sequences (100000020 bp) in 2.945 CPU sec, 3.331 real sec...
[M::process] read 672954 sequences (100000193 bp) in 1.976 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285058, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (267.83, 134.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673244 reads in 243.626 CPU sec, 7.813 real sec
sorting block 31, #reads = 1079259 mem = 536880615 peak = 918908928
[M::process] read 672862 sequences (100000184 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284821, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.55, 136.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673102 reads in 140.451 CPU sec, 4.733 real sec
[M::mem_process_seqs] Processed 673168 reads in 330.955 CPU sec, 10.893 real sec
sorting block 32, #reads = 1079599 mem = 536884901 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284534, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.70, 138.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672856 sequences (100000041 bp) in 2.695 CPU sec, 2.932 real sec...
[M::process] read 672768 sequences (100000277 bp) in 1.900 CPU sec, 1.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284714, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.84, 143.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 23, 171)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (46.08, 66.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672954 reads in 267.274 CPU sec, 8.594 real sec
[M::mem_process_seqs] Processed 672862 reads in 147.027 CPU sec, 4.747 real sec
sorting block 33, #reads = 1079447 mem = 536879540 peak = 918908928
[M::process] read 672768 sequences (100000024 bp) in 2.366 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284108, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.07, 144.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 763, 1417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3881)
[M::mem_pestat] mean and std.dev: (812.77, 708.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5113)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672646 sequences (100000047 bp) in 1.407 CPU sec, 1.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284207, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (281.31, 146.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 22, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233)
[M::mem_pestat] mean and std.dev: (34.89, 29.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 306)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284302, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (279.96, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 30, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (84.25, 80.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 696)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672856 reads in 373.317 CPU sec, 12.044 real sec
[M::mem_process_seqs] Processed 672768 reads in 267.608 CPU sec, 8.702 real sec
sorting block 34, #reads = 1079360 mem = 536878724 peak = 918908928
[M::mem_process_seqs] Processed 672768 reads in 282.737 CPU sec, 9.246 real sec
[M::process] read 672618 sequences (100000107 bp) in 2.705 CPU sec, 2.970 real sec...
sorting block 35, #reads = 1079366 mem = 536878289 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283740, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 205, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (281.26, 144.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672792 sequences (100000142 bp) in 2.269 CPU sec, 2.397 real sec...
[M::process] read 673068 sequences (100000244 bp) in 1.730 CPU sec, 1.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283795, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (284.14, 148.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 71, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2035)
[M::mem_pestat] mean and std.dev: (291.70, 321.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2705)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672646 reads in 267.475 CPU sec, 8.599 real sec
[M::mem_process_seqs] Processed 672618 reads in 156.548 CPU sec, 5.052 real sec
sorting block 36, #reads = 1078719 mem = 536878859 peak = 918908928
[M::process] read 672896 sequences (100000098 bp) in 2.885 CPU sec, 3.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283757, 15, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (281.51, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 169, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (175.00, 135.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672772 sequences (100000041 bp) in 1.616 CPU sec, 1.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284523, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.94, 141.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 73, 76)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222)
[M::mem_pestat] mean and std.dev: (36.88, 31.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 295)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284495, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (276.20, 143.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672792 reads in 374.389 CPU sec, 12.058 real sec
[M::mem_process_seqs] Processed 673068 reads in 263.913 CPU sec, 8.555 real sec
sorting block 37, #reads = 1079288 mem = 536878287 peak = 918908928
[M::mem_process_seqs] Processed 672896 reads in 277.856 CPU sec, 9.052 real sec
sorting block 38, #reads = 1078974 mem = 536878676 peak = 918908928
[M::process] read 672730 sequences (100000237 bp) in 2.431 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284608, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.62, 144.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 668, 7376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21792)
[M::mem_pestat] mean and std.dev: (2701.50, 3112.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29000)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672548 sequences (100000245 bp) in 2.661 CPU sec, 2.971 real sec...
[M::process] read 673274 sequences (100000214 bp) in 1.582 CPU sec, 1.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284813, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.45, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 241, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (203.20, 143.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672772 reads in 259.141 CPU sec, 8.354 real sec
[M::mem_process_seqs] Processed 672730 reads in 153.516 CPU sec, 4.978 real sec
sorting block 39, #reads = 1079023 mem = 536880367 peak = 918908928
[M::process] read 673350 sequences (100000196 bp) in 2.379 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284465, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 206, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (284.52, 149.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673214 sequences (100000060 bp) in 1.534 CPU sec, 1.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284363, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.21, 139.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284483, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.21, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 98, 1208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3546)
[M::mem_pestat] mean and std.dev: (516.75, 566.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4715)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672548 reads in 365.412 CPU sec, 11.775 real sec
[M::mem_process_seqs] Processed 673350 reads in 265.071 CPU sec, 8.603 real sec
sorting block 40, #reads = 1079233 mem = 536878453 peak = 918908928
[M::mem_process_seqs] Processed 673274 reads in 279.603 CPU sec, 9.125 real sec
sorting block 41, #reads = 1079610 mem = 536879095 peak = 918908928
[M::process] read 673236 sequences (100000140 bp) in 2.785 CPU sec, 3.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283917, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.85, 141.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673134 sequences (100000150 bp) in 2.712 CPU sec, 3.101 real sec...
[M::process] read 673142 sequences (100000012 bp) in 1.444 CPU sec, 1.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284142, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.58, 141.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673214 reads in 265.876 CPU sec, 8.593 real sec
[M::mem_process_seqs] Processed 673236 reads in 153.303 CPU sec, 4.952 real sec
sorting block 42, #reads = 1079353 mem = 536876944 peak = 918908928
[M::process] read 672940 sequences (100000271 bp) in 2.842 CPU sec, 3.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284309, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.53, 140.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672898 sequences (100000211 bp) in 1.641 CPU sec, 1.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284458, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.42, 141.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673134 reads in 272.276 CPU sec, 8.785 real sec
sorting block 43, #reads = 1079395 mem = 536879202 peak = 918908928
[M::mem_process_seqs] Processed 673142 reads in 156.748 CPU sec, 5.091 real sec
sorting block 44, #reads = 1079365 mem = 536876644 peak = 918908928
[M::process] read 672780 sequences (100000098 bp) in 1.875 CPU sec, 1.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283946, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.43, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672854 sequences (100000127 bp) in 1.519 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283716, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.77, 146.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283677, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.71, 150.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672940 reads in 379.554 CPU sec, 12.265 real sec
[M::mem_process_seqs] Processed 672898 reads in 267.544 CPU sec, 8.704 real sec
[M::mem_process_seqs] Processed 672780 reads in 280.361 CPU sec, 9.167 real sec
sorting block 45, #reads = 1078038 mem = 536880594 peak = 918908928
[M::process] read 672668 sequences (100000099 bp) in 2.404 CPU sec, 2.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284178, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.73, 149.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1078462 mem = 536877685 peak = 918908928
[M::process] read 673000 sequences (100000011 bp) in 2.790 CPU sec, 2.992 real sec...
[M::process] read 673110 sequences (100000082 bp) in 2.037 CPU sec, 2.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283402, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (286.33, 154.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672854 reads in 285.248 CPU sec, 9.173 real sec
[M::mem_process_seqs] Processed 672668 reads in 170.538 CPU sec, 5.510 real sec
sorting block 47, #reads = 1078651 mem = 536878441 peak = 918908928
[M::process] read 673134 sequences (100000104 bp) in 3.730 CPU sec, 4.152 real sec...
[M::process] read 673014 sequences (100000102 bp) in 1.247 CPU sec, 1.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283747, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.56, 144.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673110 reads in 147.889 CPU sec, 4.777 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283889, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.23, 148.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 87, 1034)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3008)
[M::mem_pestat] mean and std.dev: (435.08, 565.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3995)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672938 sequences (100000171 bp) in 1.489 CPU sec, 1.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284109, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.15, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 256, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226)
[M::mem_pestat] mean and std.dev: (354.40, 343.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2955)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673000 reads in 426.656 CPU sec, 13.752 real sec
sorting block 48, #reads = 1079363 mem = 536879029 peak = 918908928
[M::mem_process_seqs] Processed 673134 reads in 177.680 CPU sec, 5.815 real sec
sorting block 49, #reads = 1079037 mem = 536879700 peak = 918908928
[M::process] read 673342 sequences (100000063 bp) in 2.517 CPU sec, 2.810 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284324, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.25, 144.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673336 sequences (100000194 bp) in 1.177 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284656, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (268.90, 141.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 23, 158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (55.92, 62.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284331, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (278.87, 149.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 87, 701)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2067)
[M::mem_pestat] mean and std.dev: (215.64, 300.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2750)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673014 reads in 384.936 CPU sec, 12.437 real sec
[M::mem_process_seqs] Processed 673342 reads in 273.749 CPU sec, 8.909 real sec
[M::mem_process_seqs] Processed 672938 reads in 290.574 CPU sec, 9.529 real sec
sorting block 50, #reads = 1079236 mem = 536880585 peak = 918908928
[M::process] read 673184 sequences (100000070 bp) in 3.233 CPU sec, 3.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284799, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.83, 139.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 79, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574)
[M::mem_pestat] mean and std.dev: (270.40, 307.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2092)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 51, #reads = 1079243 mem = 536877951 peak = 918908928
[M::process] read 673060 sequences (100000120 bp) in 3.005 CPU sec, 3.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284619, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.40, 140.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673336 reads in 266.149 CPU sec, 8.620 real sec
[M::mem_process_seqs] Processed 673184 reads in 165.195 CPU sec, 5.316 real sec
[M::process] read 673128 sequences (100000014 bp) in 2.237 CPU sec, 2.375 real sec...
sorting block 52, #reads = 1078700 mem = 536877237 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284127, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.51, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 546, 4303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12869)
[M::mem_pestat] mean and std.dev: (2471.30, 2992.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17152)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673128 sequences (100000159 bp) in 3.318 CPU sec, 3.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284463, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.59, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 150, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (131.29, 87.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672964 sequences (100000120 bp) in 2.063 CPU sec, 2.064 real sec...
[M::mem_process_seqs] Processed 673060 reads in 256.943 CPU sec, 8.242 real sec
[M::mem_process_seqs] Processed 673128 reads in 149.560 CPU sec, 4.827 real sec
sorting block 53, #reads = 1077778 mem = 536880737 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284829, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.37, 143.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 87, 132)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222)
[M::mem_pestat] mean and std.dev: (104.64, 53.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 317)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672790 sequences (100000162 bp) in 3.105 CPU sec, 3.423 real sec...
[M::process] read 672798 sequences (100000140 bp) in 2.144 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284548, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (275.94, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 331, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2307)
[M::mem_pestat] mean and std.dev: (269.11, 307.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3068)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673128 reads in 248.495 CPU sec, 8.002 real sec
[M::mem_process_seqs] Processed 672964 reads in 148.657 CPU sec, 4.822 real sec
sorting block 54, #reads = 1076564 mem = 536876621 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284465, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.34, 148.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672790 sequences (100000250 bp) in 3.078 CPU sec, 3.658 real sec...
[M::process] read 672832 sequences (100000191 bp) in 1.458 CPU sec, 1.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283900, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (283.32, 151.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672790 reads in 266.758 CPU sec, 8.595 real sec
sorting block 55, #reads = 1077271 mem = 536881079 peak = 918908928
[M::mem_process_seqs] Processed 672798 reads in 160.374 CPU sec, 5.193 real sec
sorting block 56, #reads = 1079260 mem = 536877771 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283968, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (285.06, 154.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 122, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (117.82, 100.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673012 sequences (100000111 bp) in 3.285 CPU sec, 3.683 real sec...
[M::process] read 673158 sequences (100000028 bp) in 2.190 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283438, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 204, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (288.33, 156.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672790 reads in 275.530 CPU sec, 8.823 real sec
[M::mem_process_seqs] Processed 672832 reads in 165.892 CPU sec, 5.366 real sec
sorting block 57, #reads = 1077965 mem = 536877975 peak = 918908928
[M::process] read 673092 sequences (100000175 bp) in 1.997 CPU sec, 2.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284012, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.77, 147.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673116 sequences (100000063 bp) in 1.529 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284319, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.06, 146.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 22, 65)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185)
[M::mem_pestat] mean and std.dev: (23.27, 22.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 245)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284422, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.67, 146.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673012 reads in 390.531 CPU sec, 12.591 real sec
[M::mem_process_seqs] Processed 673158 reads in 277.541 CPU sec, 9.016 real sec
[M::mem_process_seqs] Processed 673092 reads in 294.879 CPU sec, 9.641 real sec
sorting block 58, #reads = 1079312 mem = 536878335 peak = 918908928
[M::process] read 673174 sequences (100000266 bp) in 2.428 CPU sec, 2.670 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284679, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (272.89, 146.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1078955 mem = 536883138 peak = 918908928
[M::process] read 673554 sequences (100000026 bp) in 3.377 CPU sec, 3.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284548, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.57, 150.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 46, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (104.00, 106.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673278 sequences (100000181 bp) in 1.495 CPU sec, 1.496 real sec...
[M::mem_process_seqs] Processed 673116 reads in 259.435 CPU sec, 8.332 real sec
[M::mem_process_seqs] Processed 673174 reads in 149.529 CPU sec, 4.821 real sec
sorting block 60, #reads = 1078523 mem = 536875594 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285557, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.76, 139.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (60, 86, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (180.92, 200.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673150 sequences (100000070 bp) in 4.379 CPU sec, 4.805 real sec...
[M::process] read 673166 sequences (100000265 bp) in 1.706 CPU sec, 1.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285161, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.60, 141.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 320, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729)
[M::mem_pestat] mean and std.dev: (161.88, 205.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2301)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673554 reads in 263.158 CPU sec, 8.430 real sec
sorting block 61, #reads = 1078196 mem = 536879233 peak = 918908928
[M::process] read 673158 sequences (100000101 bp) in 1.525 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285093, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.26, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 169, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (166.08, 184.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673150 reads in 139.523 CPU sec, 4.507 real sec
[M::mem_process_seqs] Processed 673278 reads in 331.382 CPU sec, 10.695 real sec
sorting block 62, #reads = 1079611 mem = 536881962 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284956, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.67, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673114 sequences (100000005 bp) in 3.230 CPU sec, 3.520 real sec...
[M::process] read 672876 sequences (100000083 bp) in 2.120 CPU sec, 2.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284899, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.83, 143.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 42, 3602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10784)
[M::mem_pestat] mean and std.dev: (1407.53, 1687.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14375)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673166 reads in 276.885 CPU sec, 8.871 real sec
[M::mem_process_seqs] Processed 673158 reads in 153.358 CPU sec, 4.929 real sec
sorting block 63, #reads = 1078808 mem = 536880367 peak = 918908928
[M::process] read 672908 sequences (100000073 bp) in 2.515 CPU sec, 2.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284387, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.76, 145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672944 sequences (100000086 bp) in 1.372 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284014, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.65, 149.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283897, 21, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (283.96, 153.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 199, 821)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2409)
[M::mem_pestat] mean and std.dev: (380.05, 377.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3203)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673114 reads in 355.664 CPU sec, 11.439 real sec
[M::mem_process_seqs] Processed 672876 reads in 259.218 CPU sec, 8.392 real sec
[M::mem_process_seqs] Processed 672908 reads in 274.627 CPU sec, 8.942 real sec
sorting block 64, #reads = 1078138 mem = 536880485 peak = 918908928
sorting block 65, #reads = 1073546 mem = 536881048 peak = 918908928
[M::process] read 673028 sequences (100000009 bp) in 2.663 CPU sec, 2.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283645, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.28, 154.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 59, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396)
[M::mem_pestat] mean and std.dev: (63.11, 60.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673038 sequences (100000068 bp) in 2.998 CPU sec, 3.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282972, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.29, 154.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 22, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1824)
[M::mem_pestat] mean and std.dev: (282.93, 390.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2426)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673156 sequences (100000082 bp) in 2.160 CPU sec, 2.154 real sec...
[M::mem_process_seqs] Processed 672944 reads in 266.574 CPU sec, 8.522 real sec
[M::mem_process_seqs] Processed 673028 reads in 164.072 CPU sec, 5.243 real sec
sorting block 66, #reads = 1077368 mem = 536880250 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283552, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.90, 149.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (87, 165, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (196.50, 185.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673122 sequences (100000280 bp) in 3.113 CPU sec, 3.404 real sec...
[M::process] read 673114 sequences (100000228 bp) in 1.670 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284329, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.71, 145.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673038 reads in 259.864 CPU sec, 8.338 real sec
[M::mem_process_seqs] Processed 673156 reads in 151.752 CPU sec, 4.914 real sec
sorting block 67, #reads = 1079376 mem = 536880367 peak = 918908928
sorting block 68, #reads = 1079760 mem = 536878592 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284045, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (275.03, 147.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673164 sequences (100000202 bp) in 2.544 CPU sec, 2.888 real sec...
[M::process] read 673414 sequences (100000024 bp) in 1.699 CPU sec, 1.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284666, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.55, 145.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 14, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231)
[M::mem_pestat] mean and std.dev: (39.80, 57.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 307)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673122 reads in 261.793 CPU sec, 8.429 real sec
[M::mem_process_seqs] Processed 673114 reads in 158.462 CPU sec, 5.131 real sec
sorting block 69, #reads = 1078664 mem = 536879115 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284966, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (275.50, 149.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673398 sequences (100000059 bp) in 2.790 CPU sec, 3.023 real sec...
[M::process] read 673242 sequences (100000134 bp) in 1.580 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284897, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (270.78, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673164 reads in 247.103 CPU sec, 7.944 real sec
[M::mem_process_seqs] Processed 673414 reads in 142.146 CPU sec, 4.595 real sec
sorting block 70, #reads = 1076137 mem = 536879192 peak = 918908928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284932, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.81, 140.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (292, 552, 1477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3847)
[M::mem_pestat] mean and std.dev: (810.85, 932.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5032)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673152 sequences (100000048 bp) in 3.033 CPU sec, 3.467 real sec...
[M::process] read 673176 sequences (100000194 bp) in 1.350 CPU sec, 1.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284758, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.87, 142.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673398 reads in 257.922 CPU sec, 8.289 real sec
[M::mem_process_seqs] Processed 673242 reads in 151.327 CPU sec, 4.895 real sec
sorting block 71, #reads = 1078477 mem = 536879150 peak = 918908928
[M::process] read 672970 sequences (100000180 bp) in 2.237 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284718, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.90, 141.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673016 sequences (100000231 bp) in 1.676 CPU sec, 1.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284123, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.36, 142.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283861, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (274.48, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673152 reads in 358.502 CPU sec, 11.523 real sec
sorting block 72, #reads = 1079740 mem = 536876974 peak = 918908928
[M::mem_process_seqs] Processed 673176 reads in 254.804 CPU sec, 8.255 real sec
[M::mem_process_seqs] Processed 672970 reads in 270.084 CPU sec, 8.800 real sec
sorting block 73, #reads = 1079618 mem = 536880301 peak = 918908928
[M::process] read 672912 sequences (100000194 bp) in 2.716 CPU sec, 3.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283858, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.06, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (61, 604, 1563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4567)
[M::mem_pestat] mean and std.dev: (767.31, 751.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6069)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672792 sequences (100000294 bp) in 2.092 CPU sec, 2.189 real sec...
[M::process] read 672892 sequences (100000033 bp) in 1.470 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283943, 20, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.56, 148.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 351, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250)
[M::mem_pestat] mean and std.dev: (217.12, 192.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1659)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673016 reads in 242.725 CPU sec, 7.771 real sec
[M::mem_process_seqs] Processed 672912 reads in 144.040 CPU sec, 4.640 real sec
sorting block 74, #reads = 1078130 mem = 536877237 peak = 918908928
sorting block 75, #reads = 1074682 mem = 536879122 peak = 918908928
[M::process] read 672844 sequences (100000103 bp) in 2.464 CPU sec, 2.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283856, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (283.76, 151.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 148, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2186)
[M::mem_pestat] mean and std.dev: (268.75, 317.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2913)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673024 sequences (100000107 bp) in 1.560 CPU sec, 1.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283597, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.65, 153.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283882, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (283.85, 151.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672792 reads in 352.185 CPU sec, 11.317 real sec
[M::mem_process_seqs] Processed 672892 reads in 250.767 CPU sec, 8.112 real sec
[M::mem_process_seqs] Processed 672844 reads in 265.727 CPU sec, 8.646 real sec
sorting block 76, #reads = 1076559 mem = 536877989 peak = 918908928
[M::process] read 673014 sequences (100000247 bp) in 2.079 CPU sec, 2.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284449, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.92, 144.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 30, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276)
[M::mem_pestat] mean and std.dev: (27.36, 29.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 367)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672946 sequences (100000297 bp) in 1.571 CPU sec, 1.732 real sec...
[M::process] read 672860 sequences (100000035 bp) in 1.160 CPU sec, 1.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283545, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (274.29, 144.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673024 reads in 214.977 CPU sec, 6.896 real sec
sorting block 77, #reads = 1078426 mem = 536878860 peak = 918908928
[M::process] read 672984 sequences (100000142 bp) in 1.433 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283842, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.69, 146.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 39, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (92.90, 123.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672946 reads in 118.863 CPU sec, 3.828 real sec
sorting block 78, #reads = 1078286 mem = 536880056 peak = 918908928
[M::mem_process_seqs] Processed 673014 reads in 276.878 CPU sec, 8.929 real sec
[M::process] read 230776 sequences (34295944 bp) in 0.771 CPU sec, 0.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283731, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.00, 145.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (44, 81, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2432)
[M::mem_pestat] mean and std.dev: (356.50, 382.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3228)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 97483, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (282.95, 153.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284039, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (281.10, 151.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672860 reads in 289.866 CPU sec, 9.419 real sec
[M::mem_process_seqs] Processed 230776 reads in 168.103 CPU sec, 5.477 real sec
sorting block 79, #reads = 1079412 mem = 536875298 peak = 918908928
[M::mem_process_seqs] Processed 672984 reads in 184.761 CPU sec, 6.075 real sec
sorting block 80, #reads = 1078880 mem = 536878400 peak = 967999488
os version: Linux 6.12.88
cpu vendor: GenuineIntel
signature: 000a06d1
features: 1f8bfbff fffab20b
extended: f1bf27eb 1b407f7e
amd bits: 2c100800 00000121
brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 87044834
overall: 69691027456 mem 16769.266 user 1271.646 sys 785.620 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L002\tPU:L002\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T18-D0003255_R1.fastq.gz 0001.T18-D0003255_R2.fastq.gz
[main] Real time: 785.649 sec; CPU: 18040.915 sec
sorting block 81, #reads = 284318 mem = 141394562 peak = 967999488
uniq header count 1
merging 82 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
signature: 000a06d1
features: 1f8bfbff fffab20b
extended: f1bf27eb 1b407f7e
amd bits: 2c100800 00000121
brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3381055223
output reads: 87678317
bam_mem_sort: 82 calls 54.932 user 1.371 sys 56.411 real
bam_write: 82 calls 30.589 user 1.923 sys 33.362 real
execute: 1 calls 29.386 user 2.098 sys 818.409 real
merge_files: 1 calls 29.368 user 2.073 sys 32.517 real
parse_chunk: 1301 calls 54.561 user 14.747 sys 70.377 real
read_chunk: 3501 calls 42.373 user 29.206 sys 541.481 real
sort_block: 1 calls 31.236 user 28.166 sys 524.491 real
write_chunk: 2198 calls 28.820 user 1.816 sys 31.203 real
overall: 2186436608 mem 982.498 user 71.479 sys 818.426 real
ls: cannot access '*.cram': No such file or directory