.command.err - Workdir - DAQ:SENTIEON_BWAMEM (T21-D0003376;L001;0002)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1e/fe7f3c123de0ad29cc37b4761f97a2/.command.err
Size: 89.6 KB
Task: DAQ:SENTIEON_BWAMEM (T21-D0003376;L001;0002)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1256.349 CPU sec, 43.983 real sec
[M::process] read 676892 sequences (100000135 bp) in 1.058 CPU sec, 1.065 real sec...
[M::process] read 676808 sequences (100000132 bp) in 1.618 CPU sec, 1.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288154, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (227.13, 106.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676696 sequences (100000165 bp) in 1.531 CPU sec, 1.541 real sec...
[M::process] read 676688 sequences (100000180 bp) in 1.554 CPU sec, 1.555 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288050, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.37, 110.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676892 reads in 174.943 CPU sec, 5.506 real sec
[M::mem_process_seqs] Processed 676808 reads in 99.287 CPU sec, 3.153 real sec
sorting block 0, #reads = 1081667 mem = 536878268 peak = 614289408
[M::process] read 676780 sequences (100000125 bp) in 1.755 CPU sec, 1.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288842, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.48, 110.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 44, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288)
[M::mem_pestat] mean and std.dev: (181.23, 233.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1708)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676884 sequences (100000187 bp) in 1.486 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287743, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.01, 113.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287753, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (228.67, 109.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676696 reads in 258.365 CPU sec, 8.266 real sec
[M::mem_process_seqs] Processed 676688 reads in 183.518 CPU sec, 5.900 real sec
[M::mem_process_seqs] Processed 676780 reads in 191.560 CPU sec, 6.173 real sec
sorting block 1, #reads = 1082055 mem = 536879649 peak = 743264256
sorting block 2, #reads = 1081744 mem = 536881064 peak = 925712384
[M::process] read 676770 sequences (100000256 bp) in 2.066 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287980, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.33, 110.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 38, 973)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2867)
[M::mem_pestat] mean and std.dev: (281.92, 401.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3814)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676824 sequences (100000294 bp) in 1.560 CPU sec, 1.557 real sec...
[M::process] read 676660 sequences (100000050 bp) in 1.560 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288091, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.03, 112.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676884 reads in 179.700 CPU sec, 5.679 real sec
[M::mem_process_seqs] Processed 676770 reads in 101.436 CPU sec, 3.194 real sec
sorting block 3, #reads = 1082343 mem = 536878105 peak = 925712384
[M::process] read 676606 sequences (100000150 bp) in 1.624 CPU sec, 1.672 real sec...
[M::process] read 676428 sequences (100000041 bp) in 1.460 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287846, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.71, 114.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676660 reads in 92.417 CPU sec, 2.959 real sec
sorting block 4, #reads = 1082377 mem = 536878270 peak = 925712384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287386, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.59, 112.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676182 sequences (100000010 bp) in 1.479 CPU sec, 1.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287896, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.07, 114.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676824 reads in 264.714 CPU sec, 8.420 real sec
[M::mem_process_seqs] Processed 676606 reads in 186.639 CPU sec, 5.959 real sec
sorting block 5, #reads = 1081012 mem = 536879652 peak = 925712384
[M::process] read 676562 sequences (100000233 bp) in 1.964 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287715, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.09, 117.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676836 sequences (100000037 bp) in 1.669 CPU sec, 1.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287799, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.48, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287864, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.63, 114.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676428 reads in 252.416 CPU sec, 8.058 real sec
[M::mem_process_seqs] Processed 676182 reads in 176.216 CPU sec, 5.647 real sec
[M::mem_process_seqs] Processed 676562 reads in 184.374 CPU sec, 5.930 real sec
sorting block 6, #reads = 1081932 mem = 536877606 peak = 925712384
sorting block 7, #reads = 1081625 mem = 536881312 peak = 954900480
[M::process] read 676682 sequences (100000036 bp) in 1.935 CPU sec, 2.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288632, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.09, 111.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676470 sequences (100000276 bp) in 1.522 CPU sec, 1.528 real sec...
[M::process] read 676458 sequences (100000175 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288009, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.04, 113.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676836 reads in 174.694 CPU sec, 5.529 real sec
[M::mem_process_seqs] Processed 676682 reads in 99.474 CPU sec, 3.140 real sec
sorting block 8, #reads = 1082039 mem = 536877931 peak = 954900480
[M::process] read 676924 sequences (100000079 bp) in 1.753 CPU sec, 1.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288036, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.07, 114.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676626 sequences (100000240 bp) in 1.570 CPU sec, 1.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288133, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.46, 116.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288152, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.74, 111.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676470 reads in 246.222 CPU sec, 7.862 real sec
[M::mem_process_seqs] Processed 676458 reads in 173.543 CPU sec, 5.561 real sec
[M::mem_process_seqs] Processed 676924 reads in 181.524 CPU sec, 5.834 real sec
sorting block 9, #reads = 1081912 mem = 536878007 peak = 954900480
sorting block 10, #reads = 1081961 mem = 536879970 peak = 966758400
[M::process] read 676652 sequences (100000041 bp) in 1.713 CPU sec, 1.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288243, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.23, 113.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 205, 628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1808)
[M::mem_pestat] mean and std.dev: (389.86, 415.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2398)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676830 sequences (100000033 bp) in 1.644 CPU sec, 1.656 real sec...
[M::process] read 676760 sequences (100000206 bp) in 1.600 CPU sec, 1.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288045, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.74, 114.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 248, 777)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2317)
[M::mem_pestat] mean and std.dev: (410.91, 422.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3087)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676626 reads in 172.178 CPU sec, 5.449 real sec
[M::process] read 676466 sequences (100000099 bp) in 1.150 CPU sec, 1.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288082, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.31, 114.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 125, 1093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3189)
[M::mem_pestat] mean and std.dev: (532.55, 512.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4237)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676830 reads in 90.682 CPU sec, 2.999 real sec
sorting block 11, #reads = 1082526 mem = 536877761 peak = 966758400
[M::mem_process_seqs] Processed 676652 reads in 206.510 CPU sec, 6.653 real sec
sorting block 12, #reads = 1082676 mem = 536880241 peak = 1017253888
[M::process] read 676282 sequences (100000141 bp) in 1.685 CPU sec, 1.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288002, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.18, 116.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 26, 719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131)
[M::mem_pestat] mean and std.dev: (114.89, 223.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2837)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676070 sequences (100000229 bp) in 1.173 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288166, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.49, 117.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287675, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.90, 119.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676760 reads in 251.518 CPU sec, 8.043 real sec
[M::mem_process_seqs] Processed 676466 reads in 169.447 CPU sec, 5.436 real sec
[M::mem_process_seqs] Processed 676282 reads in 177.126 CPU sec, 5.700 real sec
sorting block 13, #reads = 1081994 mem = 536877571 peak = 1017253888
sorting block 14, #reads = 1082019 mem = 536878485 peak = 1021878272
[M::process] read 676712 sequences (100000245 bp) in 2.345 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287736, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.03, 121.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676712 sequences (100000148 bp) in 1.620 CPU sec, 1.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288473, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.69, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (51, 169, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (225.60, 228.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676070 reads in 165.529 CPU sec, 5.233 real sec
[M::mem_process_seqs] Processed 676712 reads in 92.569 CPU sec, 2.898 real sec
[M::process] read 676598 sequences (100000178 bp) in 1.624 CPU sec, 1.652 real sec...
sorting block 15, #reads = 1080965 mem = 536881308 peak = 1021878272
[M::process] read 676402 sequences (100000038 bp) in 1.488 CPU sec, 1.554 real sec...
[M::process] read 676580 sequences (100000269 bp) in 1.210 CPU sec, 1.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288511, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.76, 114.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676598 reads in 82.900 CPU sec, 2.650 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288774, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.58, 113.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1081683 mem = 536878254 peak = 1021878272
[M::process] read 676810 sequences (100000108 bp) in 1.681 CPU sec, 1.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288007, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.67, 118.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676712 reads in 244.162 CPU sec, 7.764 real sec
[M::mem_process_seqs] Processed 676402 reads in 175.466 CPU sec, 5.586 real sec
sorting block 17, #reads = 1081887 mem = 536878832 peak = 1021878272
[M::process] read 676596 sequences (100000161 bp) in 1.600 CPU sec, 1.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288497, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.64, 115.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676552 sequences (100000206 bp) in 1.200 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288133, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.29, 111.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288004, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.03, 115.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (60, 323, 867)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2481)
[M::mem_pestat] mean and std.dev: (344.00, 341.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3288)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676580 reads in 242.436 CPU sec, 7.758 real sec
[M::mem_process_seqs] Processed 676810 reads in 169.109 CPU sec, 5.438 real sec
[M::mem_process_seqs] Processed 676596 reads in 176.702 CPU sec, 5.699 real sec
sorting block 18, #reads = 1081930 mem = 536879834 peak = 1021878272
sorting block 19, #reads = 1082027 mem = 536877340 peak = 1050050560
[M::process] read 676420 sequences (100000158 bp) in 2.411 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287859, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.07, 115.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676488 sequences (100000002 bp) in 1.600 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286321, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.90, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676552 reads in 167.709 CPU sec, 5.304 real sec
[M::mem_process_seqs] Processed 676420 reads in 97.733 CPU sec, 3.060 real sec
[M::process] read 676318 sequences (100000017 bp) in 1.661 CPU sec, 1.681 real sec...
sorting block 20, #reads = 1082279 mem = 536878225 peak = 1050050560
[M::process] read 676094 sequences (100000031 bp) in 1.769 CPU sec, 1.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287857, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.39, 116.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676258 sequences (100000240 bp) in 1.580 CPU sec, 1.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288008, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.48, 121.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287751, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.23, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 146, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1943)
[M::mem_pestat] mean and std.dev: (329.67, 386.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2567)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676488 reads in 243.503 CPU sec, 7.774 real sec
[M::mem_process_seqs] Processed 676094 reads in 170.072 CPU sec, 5.446 real sec
[M::mem_process_seqs] Processed 676318 reads in 177.959 CPU sec, 5.716 real sec
sorting block 21, #reads = 1079281 mem = 536880440 peak = 1050050560
sorting block 22, #reads = 1082022 mem = 536876532 peak = 1050050560
[M::process] read 676806 sequences (100000248 bp) in 1.791 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288247, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (237.86, 117.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676498 sequences (100000135 bp) in 1.363 CPU sec, 1.372 real sec...
[M::process] read 676272 sequences (100000211 bp) in 1.220 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288596, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.51, 110.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676258 reads in 167.712 CPU sec, 5.336 real sec
[M::mem_process_seqs] Processed 676806 reads in 94.630 CPU sec, 3.001 real sec
sorting block 23, #reads = 1081514 mem = 536878953 peak = 1050050560
[M::process] read 676282 sequences (100000051 bp) in 2.134 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288320, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.40, 114.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676898 sequences (100000149 bp) in 1.610 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287813, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.55, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287872, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.49, 119.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676498 reads in 240.706 CPU sec, 7.674 real sec
[M::mem_process_seqs] Processed 676272 reads in 168.544 CPU sec, 5.388 real sec
sorting block 24, #reads = 1082424 mem = 536879345 peak = 1050050560
[M::mem_process_seqs] Processed 676282 reads in 176.197 CPU sec, 5.648 real sec
sorting block 25, #reads = 1081556 mem = 536877566 peak = 1050050560
[M::process] read 676756 sequences (100000055 bp) in 1.797 CPU sec, 1.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288459, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.32, 109.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 773, 976)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2920)
[M::mem_pestat] mean and std.dev: (488.70, 453.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3892)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676434 sequences (100000198 bp) in 1.690 CPU sec, 1.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288027, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.69, 112.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 51, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276)
[M::mem_pestat] mean and std.dev: (52.17, 48.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 363)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676352 sequences (100000073 bp) in 1.630 CPU sec, 1.628 real sec...
[M::mem_process_seqs] Processed 676898 reads in 170.030 CPU sec, 5.368 real sec
[M::mem_process_seqs] Processed 676756 reads in 95.579 CPU sec, 3.002 real sec
sorting block 26, #reads = 1083022 mem = 536881114 peak = 1050050560
[M::process] read 676538 sequences (100000294 bp) in 1.861 CPU sec, 1.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288497, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (235.01, 114.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 46, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148)
[M::mem_pestat] mean and std.dev: (220.64, 308.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2860)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676392 sequences (100000281 bp) in 1.570 CPU sec, 1.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287836, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (236.10, 116.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 1405, 1405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4133)
[M::mem_pestat] mean and std.dev: (722.00, 683.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5497)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287563, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.73, 116.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 91, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1222)
[M::mem_pestat] mean and std.dev: (187.67, 230.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1622)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676434 reads in 247.805 CPU sec, 7.932 real sec
[M::mem_process_seqs] Processed 676352 reads in 171.956 CPU sec, 5.512 real sec
sorting block 27, #reads = 1082620 mem = 536878059 peak = 1050050560
[M::mem_process_seqs] Processed 676538 reads in 179.694 CPU sec, 5.773 real sec
sorting block 28, #reads = 1082175 mem = 536878909 peak = 1114836992
[M::process] read 676230 sequences (100000031 bp) in 2.346 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287573, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.53, 116.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676118 sequences (100000221 bp) in 1.560 CPU sec, 1.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287859, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.30, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676392 reads in 171.887 CPU sec, 5.436 real sec
[M::process] read 676906 sequences (100000166 bp) in 1.645 CPU sec, 1.645 real sec...
[M::mem_process_seqs] Processed 676230 reads in 94.383 CPU sec, 2.952 real sec
sorting block 29, #reads = 1082384 mem = 536877933 peak = 1114836992
[M::process] read 677294 sequences (100000260 bp) in 2.003 CPU sec, 2.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287415, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.30, 121.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676862 sequences (100000105 bp) in 1.480 CPU sec, 1.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288141, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.21, 113.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676118 reads in 169.974 CPU sec, 5.401 real sec
[M::mem_process_seqs] Processed 676906 reads in 96.417 CPU sec, 3.067 real sec
sorting block 30, #reads = 1081736 mem = 536877138 peak = 1114836992
sorting block 31, #reads = 1081759 mem = 536879507 peak = 1132183552
[M::process] read 676944 sequences (100000034 bp) in 1.876 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288107, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.86, 110.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676980 sequences (100000205 bp) in 1.630 CPU sec, 1.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287849, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.01, 112.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287779, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.44, 113.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677294 reads in 254.087 CPU sec, 8.114 real sec
[M::mem_process_seqs] Processed 676862 reads in 175.929 CPU sec, 5.640 real sec
[M::mem_process_seqs] Processed 676944 reads in 183.752 CPU sec, 5.905 real sec
sorting block 32, #reads = 1082169 mem = 536879129 peak = 1132183552
sorting block 33, #reads = 1082190 mem = 536878001 peak = 1132183552
[M::process] read 677170 sequences (100000234 bp) in 2.097 CPU sec, 2.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287602, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.33, 109.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 41, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160)
[M::mem_pestat] mean and std.dev: (278.00, 356.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2879)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677022 sequences (100000149 bp) in 1.350 CPU sec, 1.347 real sec...
[M::process] read 676978 sequences (100000282 bp) in 1.450 CPU sec, 1.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288046, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.53, 107.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676980 reads in 180.290 CPU sec, 5.711 real sec
[M::mem_process_seqs] Processed 677170 reads in 101.214 CPU sec, 3.205 real sec
sorting block 34, #reads = 1082335 mem = 536877497 peak = 1132183552
[M::process] read 676932 sequences (100000123 bp) in 1.765 CPU sec, 1.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287776, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.04, 107.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676668 sequences (100000009 bp) in 1.510 CPU sec, 1.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287784, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.16, 109.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 91, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322)
[M::mem_pestat] mean and std.dev: (63.00, 40.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288105, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.88, 108.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677022 reads in 251.080 CPU sec, 8.024 real sec
[M::mem_process_seqs] Processed 676978 reads in 176.142 CPU sec, 5.649 real sec
[M::mem_process_seqs] Processed 676932 reads in 183.689 CPU sec, 5.905 real sec
sorting block 35, #reads = 1082430 mem = 536879627 peak = 1132183552
sorting block 36, #reads = 1082474 mem = 536878180 peak = 1132183552
[M::process] read 676592 sequences (100000273 bp) in 2.146 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287657, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.19, 113.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676618 sequences (100000108 bp) in 1.330 CPU sec, 1.335 real sec...
[M::process] read 676560 sequences (100000007 bp) in 1.240 CPU sec, 1.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287556, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.30, 114.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676668 reads in 182.926 CPU sec, 5.800 real sec
[M::mem_process_seqs] Processed 676592 reads in 101.860 CPU sec, 3.230 real sec
sorting block 37, #reads = 1082664 mem = 536878294 peak = 1132183552
[M::process] read 677190 sequences (100000041 bp) in 1.786 CPU sec, 1.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287706, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.66, 117.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677100 sequences (100000160 bp) in 1.590 CPU sec, 1.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288142, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.00, 109.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 24, 990)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2944)
[M::mem_pestat] mean and std.dev: (404.50, 735.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3921)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287709, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.74, 116.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676618 reads in 256.251 CPU sec, 8.186 real sec
[M::mem_process_seqs] Processed 677190 reads in 177.318 CPU sec, 5.687 real sec
[M::mem_process_seqs] Processed 676560 reads in 185.265 CPU sec, 5.957 real sec
sorting block 38, #reads = 1082451 mem = 536879909 peak = 1132183552
sorting block 39, #reads = 1082020 mem = 536879654 peak = 1132183552
[M::process] read 676854 sequences (100000098 bp) in 2.137 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288209, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.49, 112.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676772 sequences (100000200 bp) in 1.388 CPU sec, 1.384 real sec...
[M::process] read 677184 sequences (100000283 bp) in 1.270 CPU sec, 1.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288211, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.11, 113.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 52, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (41.75, 35.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 369)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677100 reads in 178.079 CPU sec, 5.670 real sec
[M::mem_process_seqs] Processed 676854 reads in 101.024 CPU sec, 3.197 real sec
sorting block 40, #reads = 1082359 mem = 536881933 peak = 1132183552
[M::process] read 677266 sequences (100000068 bp) in 2.082 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288091, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.25, 115.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676958 sequences (100000156 bp) in 1.789 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288105, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.93, 108.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288658, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (224.86, 107.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 51, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 113)
[M::mem_pestat] mean and std.dev: (39.89, 27.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 152)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676772 reads in 263.111 CPU sec, 8.393 real sec
[M::mem_process_seqs] Processed 677184 reads in 184.381 CPU sec, 5.908 real sec
sorting block 41, #reads = 1082438 mem = 536879740 peak = 1132183552
[M::mem_process_seqs] Processed 677266 reads in 192.290 CPU sec, 6.176 real sec
sorting block 42, #reads = 1082331 mem = 536879021 peak = 1132183552
[M::process] read 676800 sequences (100000275 bp) in 2.354 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287700, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.13, 108.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676954 sequences (100000147 bp) in 1.610 CPU sec, 1.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283681, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.73, 110.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 20, 27)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 73)
[M::mem_pestat] mean and std.dev: (20.40, 18.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 96)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676958 reads in 173.177 CPU sec, 5.470 real sec
[M::process] read 676936 sequences (100000179 bp) in 1.726 CPU sec, 1.724 real sec...
[M::mem_process_seqs] Processed 676800 reads in 98.440 CPU sec, 3.082 real sec
sorting block 43, #reads = 1082510 mem = 536881227 peak = 1132183552
[M::process] read 676686 sequences (100000011 bp) in 2.022 CPU sec, 2.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288028, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.73, 110.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676510 sequences (100000056 bp) in 1.670 CPU sec, 1.669 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287569, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.84, 116.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288034, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.51, 112.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676954 reads in 251.531 CPU sec, 8.024 real sec
[M::mem_process_seqs] Processed 676686 reads in 174.383 CPU sec, 5.579 real sec
sorting block 44, #reads = 1077772 mem = 536879923 peak = 1132183552
[M::mem_process_seqs] Processed 676936 reads in 181.605 CPU sec, 5.824 real sec
sorting block 45, #reads = 1082160 mem = 536877763 peak = 1132183552
[M::process] read 677038 sequences (100000043 bp) in 2.302 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287863, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.12, 118.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676798 sequences (100000050 bp) in 1.690 CPU sec, 1.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288107, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.51, 111.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676510 reads in 171.765 CPU sec, 5.434 real sec
[M::mem_process_seqs] Processed 677038 reads in 94.768 CPU sec, 2.971 real sec
[M::process] read 676842 sequences (100000106 bp) in 1.721 CPU sec, 1.726 real sec...
sorting block 46, #reads = 1082572 mem = 536879914 peak = 1155637248
[M::process] read 676692 sequences (100000295 bp) in 2.042 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288286, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.07, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 348, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (220.17, 208.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676928 sequences (100000204 bp) in 1.620 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287535, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.49, 117.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288015, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.00, 115.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676798 reads in 248.079 CPU sec, 7.917 real sec
sorting block 47, #reads = 1082108 mem = 536880148 peak = 1155637248
[M::mem_process_seqs] Processed 676692 reads in 173.711 CPU sec, 5.559 real sec
[M::mem_process_seqs] Processed 676842 reads in 181.506 CPU sec, 5.826 real sec
sorting block 48, #reads = 1082009 mem = 536880385 peak = 1155637248
[M::process] read 676968 sequences (100000030 bp) in 2.552 CPU sec, 2.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288210, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (228.96, 110.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676918 sequences (100000009 bp) in 1.670 CPU sec, 1.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288261, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.09, 107.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676928 reads in 170.242 CPU sec, 5.426 real sec
sorting block 49, #reads = 1081922 mem = 536878934 peak = 1155637248
[M::process] read 676930 sequences (100000217 bp) in 1.817 CPU sec, 1.827 real sec...
[M::process] read 676922 sequences (100000073 bp) in 1.532 CPU sec, 1.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287975, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (226.67, 109.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676918 reads in 89.407 CPU sec, 2.830 real sec
sorting block 50, #reads = 1081933 mem = 536878785 peak = 1155637248
[M::mem_process_seqs] Processed 676968 reads in 203.695 CPU sec, 6.432 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288513, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (228.05, 110.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676814 sequences (100000062 bp) in 2.051 CPU sec, 2.100 real sec...
[M::process] read 676726 sequences (100000208 bp) in 1.620 CPU sec, 1.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288000, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.18, 112.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676930 reads in 174.504 CPU sec, 5.533 real sec
[M::mem_process_seqs] Processed 676922 reads in 94.895 CPU sec, 3.011 real sec
sorting block 51, #reads = 1082016 mem = 536877595 peak = 1155637248
[M::process] read 676532 sequences (100000045 bp) in 2.058 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287946, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.89, 112.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677028 sequences (100000003 bp) in 1.620 CPU sec, 1.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287677, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.95, 115.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288049, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.00, 117.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 195, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359)
[M::mem_pestat] mean and std.dev: (125.30, 78.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676814 reads in 248.372 CPU sec, 7.922 real sec
sorting block 52, #reads = 1082043 mem = 536878215 peak = 1155637248
[M::mem_process_seqs] Processed 676726 reads in 172.545 CPU sec, 5.525 real sec
[M::mem_process_seqs] Processed 676532 reads in 180.504 CPU sec, 5.797 real sec
sorting block 53, #reads = 1081963 mem = 536878765 peak = 1181708288
[M::process] read 676958 sequences (100000068 bp) in 2.248 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287793, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.48, 114.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 235, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (184.90, 176.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1333)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676762 sequences (100000019 bp) in 1.700 CPU sec, 1.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287730, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (232.93, 116.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677028 reads in 174.515 CPU sec, 5.515 real sec
[M::process] read 676600 sequences (100000196 bp) in 1.657 CPU sec, 1.658 real sec...
[M::mem_process_seqs] Processed 676958 reads in 99.805 CPU sec, 3.124 real sec
sorting block 54, #reads = 1081098 mem = 536877572 peak = 1181708288
sorting block 55, #reads = 1082147 mem = 536879108 peak = 1181708288
[M::process] read 676574 sequences (100000287 bp) in 1.976 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287210, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.12, 117.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 11, 1517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4529)
[M::mem_pestat] mean and std.dev: (508.40, 766.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6035)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676798 sequences (100000138 bp) in 1.580 CPU sec, 1.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287707, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.27, 117.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288040, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.17, 114.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676762 reads in 260.778 CPU sec, 8.330 real sec
[M::mem_process_seqs] Processed 676600 reads in 181.302 CPU sec, 5.810 real sec
[M::mem_process_seqs] Processed 676574 reads in 189.479 CPU sec, 6.090 real sec
sorting block 56, #reads = 1081888 mem = 536879958 peak = 1181708288
[M::process] read 676820 sequences (100000200 bp) in 2.450 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287971, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.35, 108.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676900 sequences (100000011 bp) in 1.590 CPU sec, 1.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288027, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.59, 111.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676798 reads in 176.080 CPU sec, 5.572 real sec
sorting block 57, #reads = 1081464 mem = 536879720 peak = 1181708288
[M::mem_process_seqs] Processed 676820 reads in 99.260 CPU sec, 3.113 real sec
[M::process] read 676736 sequences (100000079 bp) in 1.686 CPU sec, 1.688 real sec...
sorting block 58, #reads = 1082519 mem = 536878359 peak = 1205497856
[M::process] read 676618 sequences (100000034 bp) in 1.652 CPU sec, 1.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288276, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.87, 112.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676532 sequences (100000234 bp) in 1.240 CPU sec, 1.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287928, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.55, 114.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287524, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.88, 115.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 429, 3160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9450)
[M::mem_pestat] mean and std.dev: (1033.12, 1270.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12595)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676900 reads in 249.788 CPU sec, 7.995 real sec
[M::mem_process_seqs] Processed 676736 reads in 174.725 CPU sec, 5.612 real sec
[M::mem_process_seqs] Processed 676618 reads in 182.182 CPU sec, 5.865 real sec
sorting block 59, #reads = 1082974 mem = 536879947 peak = 1205497856
[M::process] read 676440 sequences (100000072 bp) in 1.816 CPU sec, 1.892 real sec...
sorting block 60, #reads = 1082274 mem = 536878088 peak = 1205497856
[M::process] read 445854 sequences (65927103 bp) in 1.184 CPU sec, 1.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287715, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.22, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 78, 1475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4269)
[M::mem_pestat] mean and std.dev: (563.30, 627.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5666)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676440 reads in 89.931 CPU sec, 2.889 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287750, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.51, 116.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 189790, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (236.40, 119.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676532 reads in 231.884 CPU sec, 7.444 real sec
[M::mem_process_seqs] Processed 445854 reads in 72.495 CPU sec, 2.345 real sec
sorting block 61, #reads = 1082451 mem = 536881516 peak = 1205497856
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67439416
overall: 69905063936 mem 10040.683 user 128.234 sys 336.857 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L001\tPU:L001\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.T21-D0003376_R1.fastq.gz 0002.T21-D0003376_R2.fastq.gz
[main] Real time: 336.900 sec; CPU: 10168.928 sec
sorting block 62, #reads = 888511 mem = 440867553 peak = 1230057472
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 2648541041
output reads: 67971341
bam_mem_sort: 63 calls 30.094 user 0.168 sys 30.357 real
bam_write: 63 calls 17.073 user 0.754 sys 18.899 real
execute: 1 calls 18.084 user 0.520 sys 357.358 real
merge_files: 1 calls 18.056 user 0.492 sys 19.730 real
parse_chunk: 1006 calls 53.818 user 10.347 sys 65.159 real
read_chunk: 2708 calls 19.916 user 15.897 sys 295.635 real
sort_block: 1 calls 14.239 user 15.536 sys 287.038 real
write_chunk: 1700 calls 17.685 user 0.343 sys 19.128 real
overall: 2468904960 mem 768.266 user 35.228 sys 357.380 real
ls: cannot access '*.cram': No such file or directory