Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/7b5d6dbbe4a76acac86f0b632e1a15/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/68/e7a83aa6445e0bb603fb4b8db4ee88/T21-D0003376/0001.T21-D0003376_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/7b5d6dbbe4a76acac86f0b632e1a15/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/68/e7a83aa6445e0bb603fb4b8db4ee88/T21-D0003376/0001.T21-D0003376_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T21-D0003376.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1601.784 CPU sec, 55.513 real sec
[M::process] read 677026 sequences (100000130 bp) in 1.246 CPU sec, 1.254 real sec...
[M::process] read 676814 sequences (100000152 bp) in 2.010 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289043, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.45, 107.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000130 bp) in 1.989 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288572, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.46, 110.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677026 reads in 180.991 CPU sec, 5.665 real sec
[M::mem_process_seqs] Processed 676814 reads in 104.868 CPU sec, 3.292 real sec
[M::process] read 676566 sequences (100000221 bp) in 2.067 CPU sec, 2.064 real sec...
sorting block 0, #reads = 1082214 mem = 536879596 peak = 653209600
[M::process] read 676942 sequences (100000289 bp) in 2.226 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288074, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.16, 111.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 79, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211)
[M::mem_pestat] mean and std.dev: (40.82, 35.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 277)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676798 sequences (100000036 bp) in 1.880 CPU sec, 1.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287895, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.71, 113.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288064, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.37, 110.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 272.209 CPU sec, 8.670 real sec
[M::mem_process_seqs] Processed 676566 reads in 191.683 CPU sec, 6.136 real sec
[M::mem_process_seqs] Processed 676942 reads in 201.334 CPU sec, 6.460 real sec
sorting block 1, #reads = 1081841 mem = 536876854 peak = 780611584
[M::process] read 676686 sequences (100000296 bp) in 2.077 CPU sec, 2.095 real sec...
sorting block 2, #reads = 1081986 mem = 536878544 peak = 966746112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287628, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.19, 110.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676832 sequences (100000106 bp) in 1.567 CPU sec, 1.563 real sec...
[M::process] read 676680 sequences (100000015 bp) in 1.417 CPU sec, 1.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287898, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (232.20, 112.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676798 reads in 195.527 CPU sec, 6.203 real sec
[M::mem_process_seqs] Processed 676686 reads in 109.356 CPU sec, 3.469 real sec
sorting block 3, #reads = 1081881 mem = 536876753 peak = 966746112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287926, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.86, 112.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 27, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (154.45, 202.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1402)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676632 sequences (100000172 bp) in 2.403 CPU sec, 2.441 real sec...
[M::process] read 676294 sequences (100000041 bp) in 1.900 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288053, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.76, 115.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676832 reads in 188.385 CPU sec, 5.970 real sec
[M::mem_process_seqs] Processed 676680 reads in 106.889 CPU sec, 3.390 real sec
sorting block 4, #reads = 1081506 mem = 536880131 peak = 966746112
[M::process] read 676184 sequences (100000091 bp) in 2.163 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288181, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.74, 115.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676622 sequences (100000209 bp) in 1.950 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287855, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (237.37, 117.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287765, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.80, 119.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 620, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2124)
[M::mem_pestat] mean and std.dev: (259.45, 325.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2830)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676632 reads in 272.306 CPU sec, 8.676 real sec
[M::mem_process_seqs] Processed 676294 reads in 190.780 CPU sec, 6.103 real sec
sorting block 5, #reads = 1082078 mem = 536881375 peak = 1019531264
[M::mem_process_seqs] Processed 676184 reads in 200.297 CPU sec, 6.426 real sec
sorting block 6, #reads = 1082165 mem = 536878568 peak = 1019531264
[M::process] read 676870 sequences (100000229 bp) in 2.541 CPU sec, 2.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287968, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.41, 114.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676676 sequences (100000024 bp) in 2.008 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288370, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.57, 110.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676622 reads in 185.699 CPU sec, 5.868 real sec
[M::mem_process_seqs] Processed 676870 reads in 103.773 CPU sec, 3.251 real sec
sorting block 7, #reads = 1081937 mem = 536878905 peak = 1019531264
[M::process] read 676586 sequences (100000083 bp) in 2.198 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287949, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.26, 113.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676478 sequences (100000005 bp) in 2.519 CPU sec, 2.677 real sec...
[M::process] read 676758 sequences (100000141 bp) in 1.950 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288025, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.47, 115.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676676 reads in 184.336 CPU sec, 5.823 real sec
[M::mem_process_seqs] Processed 676586 reads in 103.549 CPU sec, 3.265 real sec
sorting block 8, #reads = 1081658 mem = 536880686 peak = 1019531264
sorting block 9, #reads = 1081819 mem = 536878693 peak = 1051054080
[M::process] read 676570 sequences (100000131 bp) in 2.376 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287997, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.56, 117.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 51, 162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (44.12, 49.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 615)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676692 sequences (100000235 bp) in 2.000 CPU sec, 2.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288137, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.60, 112.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288178, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (233.09, 113.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676478 reads in 272.490 CPU sec, 8.680 real sec
[M::mem_process_seqs] Processed 676758 reads in 190.381 CPU sec, 6.075 real sec
[M::mem_process_seqs] Processed 676570 reads in 199.231 CPU sec, 6.374 real sec
sorting block 10, #reads = 1082291 mem = 536881142 peak = 1051054080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288135, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.36, 115.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676672 sequences (100000169 bp) in 3.125 CPU sec, 3.174 real sec...
[M::mem_process_seqs] Processed 676692 reads in 101.690 CPU sec, 3.313 real sec
sorting block 11, #reads = 1082340 mem = 536880098 peak = 1051054080
[M::process] read 676584 sequences (100000044 bp) in 2.407 CPU sec, 2.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287731, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.61, 114.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676550 sequences (100000016 bp) in 2.010 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287961, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.93, 116.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676672 reads in 188.280 CPU sec, 5.897 real sec
[M::mem_process_seqs] Processed 676584 reads in 106.544 CPU sec, 3.338 real sec
[M::process] read 676376 sequences (100000214 bp) in 2.143 CPU sec, 2.153 real sec...
sorting block 12, #reads = 1082402 mem = 536878628 peak = 1051054080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288069, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.34, 117.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 14, 26)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 50)
[M::mem_pestat] mean and std.dev: (15.00, 7.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 62)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676048 sequences (100000106 bp) in 2.755 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287835, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (240.08, 120.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676874 sequences (100000072 bp) in 2.020 CPU sec, 2.022 real sec...
[M::mem_process_seqs] Processed 676550 reads in 185.585 CPU sec, 5.874 real sec
sorting block 13, #reads = 1082083 mem = 536880246 peak = 1051054080
[M::mem_process_seqs] Processed 676376 reads in 104.788 CPU sec, 3.292 real sec
sorting block 14, #reads = 1081767 mem = 536877848 peak = 1087537152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287761, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (242.09, 121.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676666 sequences (100000102 bp) in 2.394 CPU sec, 2.415 real sec...
[M::process] read 676476 sequences (100000023 bp) in 2.060 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288432, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.98, 112.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676048 reads in 181.255 CPU sec, 5.736 real sec
[M::mem_process_seqs] Processed 676874 reads in 102.035 CPU sec, 3.220 real sec
sorting block 15, #reads = 1081313 mem = 536879319 peak = 1087537152
[M::process] read 676582 sequences (100000191 bp) in 2.739 CPU sec, 2.769 real sec...
[M::process] read 676504 sequences (100000003 bp) in 2.020 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288113, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.53, 115.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676476 reads in 94.055 CPU sec, 2.948 real sec
sorting block 16, #reads = 1081848 mem = 536880198 peak = 1087537152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288665, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.77, 113.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 45, 45)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 129)
[M::mem_pestat] mean and std.dev: (27.89, 19.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 171)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676760 sequences (100000235 bp) in 1.981 CPU sec, 1.990 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288253, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (238.29, 118.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676666 reads in 274.362 CPU sec, 8.676 real sec
[M::mem_process_seqs] Processed 676582 reads in 114.771 CPU sec, 3.643 real sec
sorting block 17, #reads = 1082328 mem = 536877463 peak = 1087537152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288536, 18, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.24, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (53, 904, 904)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2606)
[M::mem_pestat] mean and std.dev: (484.19, 421.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3457)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676612 sequences (100000245 bp) in 2.660 CPU sec, 2.686 real sec...
[M::process] read 676596 sequences (100000112 bp) in 2.030 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288454, 14, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.49, 111.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 62, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207)
[M::mem_pestat] mean and std.dev: (186.79, 222.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1607)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676504 reads in 181.526 CPU sec, 5.740 real sec
[M::mem_process_seqs] Processed 676760 reads in 103.356 CPU sec, 3.259 real sec
sorting block 18, #reads = 1082398 mem = 536879095 peak = 1087537152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288188, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.71, 114.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676504 sequences (100000091 bp) in 2.534 CPU sec, 2.563 real sec...
[M::process] read 676502 sequences (100000001 bp) in 2.030 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288376, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.89, 115.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 398, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154)
[M::mem_pestat] mean and std.dev: (247.94, 210.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1532)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676612 reads in 184.257 CPU sec, 5.828 real sec
[M::mem_process_seqs] Processed 676596 reads in 102.714 CPU sec, 3.245 real sec
sorting block 19, #reads = 1082348 mem = 536878683 peak = 1087537152
[M::process] read 676250 sequences (100000021 bp) in 2.096 CPU sec, 2.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286119, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.85, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676094 sequences (100000196 bp) in 1.530 CPU sec, 1.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288049, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.20, 116.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287922, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (239.50, 120.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676504 reads in 271.170 CPU sec, 8.668 real sec
[M::mem_process_seqs] Processed 676502 reads in 191.358 CPU sec, 6.121 real sec
sorting block 20, #reads = 1082095 mem = 536875801 peak = 1087537152
[M::mem_process_seqs] Processed 676250 reads in 200.423 CPU sec, 6.428 real sec
sorting block 21, #reads = 1080007 mem = 536880274 peak = 1133039616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287690, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.35, 121.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676186 sequences (100000176 bp) in 3.195 CPU sec, 3.309 real sec...
[M::mem_process_seqs] Processed 676094 reads in 102.087 CPU sec, 3.334 real sec
sorting block 22, #reads = 1082188 mem = 536878012 peak = 1133039616
[M::process] read 676774 sequences (100000299 bp) in 2.565 CPU sec, 2.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287941, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (238.35, 118.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (377, 571, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109)
[M::mem_pestat] mean and std.dev: (494.42, 179.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1353)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676534 sequences (100000160 bp) in 2.020 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288601, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (230.29, 109.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676186 reads in 183.104 CPU sec, 5.740 real sec
[M::mem_process_seqs] Processed 676774 reads in 103.825 CPU sec, 3.253 real sec
[M::process] read 676312 sequences (100000181 bp) in 2.222 CPU sec, 2.250 real sec...
sorting block 23, #reads = 1081236 mem = 536879676 peak = 1133039616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288380, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (234.18, 113.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676224 sequences (100000172 bp) in 2.712 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288755, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.33, 115.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676822 sequences (100000264 bp) in 2.000 CPU sec, 2.000 real sec...
[M::mem_process_seqs] Processed 676534 reads in 183.971 CPU sec, 5.814 real sec
[M::mem_process_seqs] Processed 676312 reads in 103.000 CPU sec, 3.238 real sec
sorting block 24, #reads = 1082107 mem = 536877374 peak = 1133039616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287969, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 180, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (239.61, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676686 sequences (100000072 bp) in 2.459 CPU sec, 2.511 real sec...
[M::process] read 676390 sequences (100000057 bp) in 2.030 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288470, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (227.60, 108.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 201, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1881)
[M::mem_pestat] mean and std.dev: (318.43, 341.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2503)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676224 reads in 185.619 CPU sec, 5.872 real sec
[M::mem_process_seqs] Processed 676822 reads in 104.720 CPU sec, 3.307 real sec
sorting block 25, #reads = 1082456 mem = 536878352 peak = 1159942144
sorting block 26, #reads = 1082067 mem = 536879407 peak = 1159942144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287761, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (232.51, 113.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676454 sequences (100000153 bp) in 2.769 CPU sec, 2.792 real sec...
[M::process] read 676564 sequences (100000104 bp) in 2.050 CPU sec, 2.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288316, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (235.62, 115.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676686 reads in 188.363 CPU sec, 5.959 real sec
[M::mem_process_seqs] Processed 676390 reads in 105.717 CPU sec, 3.332 real sec
sorting block 27, #reads = 1082393 mem = 536878152 peak = 1159942144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287757, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.02, 115.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (26, 224, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (137.71, 100.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676498 sequences (100000244 bp) in 2.534 CPU sec, 2.558 real sec...
[M::process] read 676298 sequences (100000282 bp) in 2.010 CPU sec, 2.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287940, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.80, 115.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676454 reads in 187.144 CPU sec, 5.931 real sec
[M::mem_process_seqs] Processed 676564 reads in 104.892 CPU sec, 3.314 real sec
sorting block 28, #reads = 1082219 mem = 536877352 peak = 1159942144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288067, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (236.07, 117.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676096 sequences (100000155 bp) in 2.803 CPU sec, 2.832 real sec...
[M::process] read 676872 sequences (100000270 bp) in 2.040 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287481, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 180, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (239.04, 119.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676498 reads in 187.033 CPU sec, 5.920 real sec
[M::mem_process_seqs] Processed 676298 reads in 105.278 CPU sec, 3.311 real sec
sorting block 29, #reads = 1082554 mem = 536879748 peak = 1159942144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287585, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 181, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (241.52, 120.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 15, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (55.25, 71.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 677)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677118 sequences (100000047 bp) in 2.427 CPU sec, 2.458 real sec...
[M::process] read 676858 sequences (100000023 bp) in 1.490 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288213, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (230.89, 113.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676096 reads in 186.319 CPU sec, 5.921 real sec
[M::mem_process_seqs] Processed 676872 reads in 106.631 CPU sec, 3.397 real sec
sorting block 30, #reads = 1081897 mem = 536881752 peak = 1160761344
sorting block 31, #reads = 1081837 mem = 536880312 peak = 1175773184
[M::process] read 676870 sequences (100000219 bp) in 2.314 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288072, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (228.50, 111.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677196 sequences (100000082 bp) in 1.550 CPU sec, 1.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287499, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.64, 113.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 336, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (236.00, 148.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287549, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (231.03, 112.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677118 reads in 287.273 CPU sec, 9.185 real sec
[M::mem_process_seqs] Processed 676870 reads in 200.003 CPU sec, 6.414 real sec
[M::mem_process_seqs] Processed 676858 reads in 209.416 CPU sec, 6.730 real sec
sorting block 32, #reads = 1082089 mem = 536878342 peak = 1175773184
[M::process] read 677108 sequences (100000288 bp) in 2.857 CPU sec, 2.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287378, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.48, 109.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1081906 mem = 536877405 peak = 1175773184
[M::process] read 676964 sequences (100000102 bp) in 2.298 CPU sec, 2.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287973, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.02, 107.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677196 reads in 199.336 CPU sec, 6.334 real sec
[M::mem_process_seqs] Processed 677108 reads in 112.510 CPU sec, 3.524 real sec
[M::process] read 677062 sequences (100000124 bp) in 2.494 CPU sec, 2.554 real sec...
sorting block 34, #reads = 1082251 mem = 536878467 peak = 1175773184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288007, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (226.25, 108.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 89, 89)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191)
[M::mem_pestat] mean and std.dev: (59.27, 31.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 242)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677058 sequences (100000047 bp) in 2.735 CPU sec, 2.765 real sec...
[M::process] read 676700 sequences (100000014 bp) in 2.060 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287747, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (227.67, 109.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 32, 37)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 101)
[M::mem_pestat] mean and std.dev: (24.18, 22.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 133)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676964 reads in 197.643 CPU sec, 6.244 real sec
[M::mem_process_seqs] Processed 677062 reads in 111.908 CPU sec, 3.515 real sec
sorting block 35, #reads = 1082494 mem = 536876745 peak = 1175773184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287779, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (226.63, 108.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676604 sequences (100000248 bp) in 2.589 CPU sec, 2.613 real sec...
[M::process] read 676524 sequences (100000163 bp) in 2.080 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287559, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.55, 113.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677058 reads in 196.502 CPU sec, 6.229 real sec
[M::mem_process_seqs] Processed 676700 reads in 111.210 CPU sec, 3.511 real sec
sorting block 36, #reads = 1082457 mem = 536880145 peak = 1175773184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287664, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.66, 114.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676752 sequences (100000149 bp) in 2.611 CPU sec, 2.635 real sec...
[M::process] read 677362 sequences (100000118 bp) in 2.090 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287405, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.61, 116.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 15)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 39)
[M::mem_pestat] mean and std.dev: (5.12, 4.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 51)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676604 reads in 196.905 CPU sec, 6.245 real sec
[M::mem_process_seqs] Processed 676524 reads in 109.912 CPU sec, 3.476 real sec
sorting block 37, #reads = 1081844 mem = 536879081 peak = 1175773184
sorting block 38, #reads = 1082024 mem = 536880934 peak = 1199546368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287571, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.46, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677110 sequences (100000069 bp) in 2.617 CPU sec, 2.634 real sec...
[M::process] read 676910 sequences (100000202 bp) in 2.110 CPU sec, 2.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287953, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.87, 108.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676752 reads in 192.103 CPU sec, 6.084 real sec
[M::mem_process_seqs] Processed 677362 reads in 107.008 CPU sec, 3.379 real sec
sorting block 39, #reads = 1081838 mem = 536878086 peak = 1199546368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287905, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (229.02, 111.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 79, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (90.60, 85.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676792 sequences (100000057 bp) in 2.609 CPU sec, 2.631 real sec...
[M::process] read 677076 sequences (100000043 bp) in 2.090 CPU sec, 2.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287714, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (230.90, 113.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677110 reads in 190.447 CPU sec, 6.035 real sec
[M::mem_process_seqs] Processed 676910 reads in 108.787 CPU sec, 3.432 real sec
sorting block 40, #reads = 1082353 mem = 536877586 peak = 1199546368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287886, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.92, 115.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (14, 28, 100)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272)
[M::mem_pestat] mean and std.dev: (28.25, 27.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 358)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677168 sequences (100000203 bp) in 2.634 CPU sec, 2.669 real sec...
[M::process] read 677070 sequences (100000052 bp) in 2.080 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288166, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.45, 107.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676792 reads in 192.599 CPU sec, 6.101 real sec
[M::mem_process_seqs] Processed 677076 reads in 107.652 CPU sec, 3.399 real sec
sorting block 41, #reads = 1082522 mem = 536878961 peak = 1199546368
[M::process] read 676762 sequences (100000208 bp) in 2.718 CPU sec, 2.758 real sec...
[M::process] read 676912 sequences (100000174 bp) in 2.080 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288174, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (225.44, 107.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677070 reads in 104.906 CPU sec, 3.295 real sec
sorting block 42, #reads = 1082271 mem = 536875368 peak = 1199546368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287884, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.89, 108.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 28, 58)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 118)
[M::mem_pestat] mean and std.dev: (36.33, 22.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 148)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283496, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.19, 108.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 100, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543)
[M::mem_pestat] mean and std.dev: (158.38, 200.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2053)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676902 sequences (100000005 bp) in 2.022 CPU sec, 2.031 real sec...
[M::mem_process_seqs] Processed 677168 reads in 292.458 CPU sec, 9.262 real sec
[M::mem_process_seqs] Processed 676762 reads in 120.827 CPU sec, 3.838 real sec
sorting block 43, #reads = 1082432 mem = 536877621 peak = 1199546368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287486, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.81, 110.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000036 bp) in 2.860 CPU sec, 2.910 real sec...
[M::process] read 676606 sequences (100000207 bp) in 2.120 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288056, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.96, 113.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676912 reads in 193.558 CPU sec, 6.127 real sec
[M::mem_process_seqs] Processed 676902 reads in 108.347 CPU sec, 3.418 real sec
sorting block 44, #reads = 1077658 mem = 536878183 peak = 1199546368
sorting block 45, #reads = 1082608 mem = 536879360 peak = 1225175040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287453, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.83, 115.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677120 sequences (100000246 bp) in 2.622 CPU sec, 2.657 real sec...
[M::process] read 676886 sequences (100000061 bp) in 2.070 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287759, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.69, 117.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 194.238 CPU sec, 6.147 real sec
[M::mem_process_seqs] Processed 676606 reads in 108.402 CPU sec, 3.429 real sec
sorting block 46, #reads = 1082088 mem = 536878944 peak = 1225175040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288542, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.11, 111.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 28, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (176.93, 232.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1413)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676796 sequences (100000105 bp) in 2.633 CPU sec, 2.660 real sec...
[M::process] read 676648 sequences (100000199 bp) in 2.120 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288084, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (231.27, 113.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677120 reads in 186.570 CPU sec, 5.906 real sec
[M::mem_process_seqs] Processed 676886 reads in 107.330 CPU sec, 3.376 real sec
sorting block 47, #reads = 1082230 mem = 536878815 peak = 1225175040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287653, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.18, 115.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676952 sequences (100000142 bp) in 2.605 CPU sec, 2.629 real sec...
[M::process] read 677136 sequences (100000282 bp) in 2.090 CPU sec, 2.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287910, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.66, 117.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676796 reads in 189.632 CPU sec, 6.002 real sec
[M::mem_process_seqs] Processed 676648 reads in 108.160 CPU sec, 3.419 real sec
sorting block 48, #reads = 1082351 mem = 536877850 peak = 1225175040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288190, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (229.27, 111.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677028 sequences (100000098 bp) in 2.641 CPU sec, 2.662 real sec...
[M::process] read 676924 sequences (100000267 bp) in 2.100 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288424, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 177, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (225.69, 107.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676952 reads in 188.882 CPU sec, 5.985 real sec
[M::mem_process_seqs] Processed 677136 reads in 108.284 CPU sec, 3.405 real sec
sorting block 49, #reads = 1081800 mem = 536876854 peak = 1225175040
sorting block 50, #reads = 1082455 mem = 536878345 peak = 1233534976
[M::process] read 676914 sequences (100000046 bp) in 2.625 CPU sec, 2.700 real sec...
[M::process] read 676744 sequences (100000167 bp) in 2.090 CPU sec, 2.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288114, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (227.47, 110.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (45, 289, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611)
[M::mem_pestat] mean and std.dev: (248.45, 252.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2133)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676924 reads in 99.350 CPU sec, 3.137 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288035, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (227.33, 109.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288382, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (228.73, 111.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676682 sequences (100000135 bp) in 2.172 CPU sec, 2.181 real sec...
[M::mem_process_seqs] Processed 677028 reads in 288.054 CPU sec, 9.110 real sec
[M::mem_process_seqs] Processed 676914 reads in 116.584 CPU sec, 3.694 real sec
sorting block 51, #reads = 1082282 mem = 536876607 peak = 1233534976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287736, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.90, 112.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676580 sequences (100000149 bp) in 2.805 CPU sec, 2.886 real sec...
[M::process] read 677040 sequences (100000049 bp) in 2.130 CPU sec, 2.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287805, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.38, 115.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 39, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (36.38, 42.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676744 reads in 192.895 CPU sec, 6.105 real sec
sorting block 52, #reads = 1082313 mem = 536882141 peak = 1233534976
[M::mem_process_seqs] Processed 676682 reads in 107.479 CPU sec, 3.386 real sec
sorting block 53, #reads = 1082167 mem = 536879719 peak = 1234100224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287964, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (233.76, 116.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677006 sequences (100000144 bp) in 2.685 CPU sec, 2.703 real sec...
[M::process] read 676726 sequences (100000135 bp) in 2.090 CPU sec, 2.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288368, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (229.60, 113.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676580 reads in 185.786 CPU sec, 5.876 real sec
[M::mem_process_seqs] Processed 677040 reads in 105.434 CPU sec, 3.322 real sec
sorting block 54, #reads = 1081236 mem = 536878240 peak = 1234100224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287821, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (232.10, 115.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (364, 713, 1378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3406)
[M::mem_pestat] mean and std.dev: (814.09, 692.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4420)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676632 sequences (100000297 bp) in 2.573 CPU sec, 2.634 real sec...
[M::process] read 676538 sequences (100000293 bp) in 2.120 CPU sec, 2.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287527, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.71, 116.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677006 reads in 193.442 CPU sec, 6.130 real sec
[M::mem_process_seqs] Processed 676726 reads in 109.155 CPU sec, 3.448 real sec
sorting block 55, #reads = 1082170 mem = 536879646 peak = 1234100224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287616, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.81, 116.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 10, 51)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 133)
[M::mem_pestat] mean and std.dev: (18.50, 16.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 174)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676956 sequences (100000119 bp) in 2.630 CPU sec, 2.696 real sec...
[M::process] read 677028 sequences (100000266 bp) in 2.080 CPU sec, 2.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287764, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.67, 112.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676632 reads in 190.583 CPU sec, 6.036 real sec
[M::mem_process_seqs] Processed 676538 reads in 106.568 CPU sec, 3.363 real sec
sorting block 56, #reads = 1081501 mem = 536881433 peak = 1234100224
[M::process] read 676810 sequences (100000168 bp) in 2.595 CPU sec, 2.672 real sec...
[M::process] read 676638 sequences (100000137 bp) in 2.110 CPU sec, 2.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287939, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (229.27, 111.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677028 reads in 99.840 CPU sec, 3.142 real sec
sorting block 57, #reads = 1081291 mem = 536879876 peak = 1249562624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288035, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (228.13, 109.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676602 sequences (100000137 bp) in 2.046 CPU sec, 2.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287802, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 178, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (229.61, 111.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 176, 1348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3990)
[M::mem_pestat] mean and std.dev: (364.89, 530.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5311)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676956 reads in 293.193 CPU sec, 9.281 real sec
[M::mem_process_seqs] Processed 676810 reads in 121.120 CPU sec, 3.843 real sec
sorting block 58, #reads = 1082612 mem = 536879139 peak = 1249562624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288070, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (231.73, 113.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676516 sequences (100000214 bp) in 2.835 CPU sec, 2.930 real sec...
[M::process] read 676494 sequences (100000215 bp) in 2.070 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287408, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (233.44, 115.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 34, 1230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3660)
[M::mem_pestat] mean and std.dev: (361.36, 532.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4875)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676638 reads in 195.859 CPU sec, 6.207 real sec
[M::mem_process_seqs] Processed 676602 reads in 110.744 CPU sec, 3.493 real sec
sorting block 59, #reads = 1082577 mem = 536879152 peak = 1249562624
sorting block 60, #reads = 1082264 mem = 536877039 peak = 1250009088
[M::process] read 446142 sequences (65965461 bp) in 1.943 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287635, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.57, 117.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287609, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (234.83, 117.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 51, 78)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166)
[M::mem_pestat] mean and std.dev: (49.55, 23.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 210)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 189351, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 179, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.42, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676516 reads in 249.781 CPU sec, 8.031 real sec
[M::mem_process_seqs] Processed 676494 reads in 164.535 CPU sec, 5.313 real sec
[M::mem_process_seqs] Processed 446142 reads in 170.591 CPU sec, 5.521 real sec
sorting block 61, #reads = 1082170 mem = 536881278 peak = 1250009088
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: 1f8bfbff fffa320b
  extended: d19f47ab 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 67440648
overall: 69935931392 mem 11350.867 user 133.811 sys 378.899 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T21-D0003376.L001\tPU:L001\tSM:T21-D0003376\tLB:T21-D0003376\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.T21-D0003376_R1.fastq.gz 0001.T21-D0003376_R2.fastq.gz
[main] Real time: 378.933 sec; CPU: 11484.682 sec
sorting block 62, #reads = 887888 mem = 440918518 peak = 1250009088
uniq header count 1
merging 63 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 00050657
  features: 1f8bfbff fffa320b
  extended: d19f47ab 00000818
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8259CL CPU @ 2.50GHz
threads: 31 max 48
algo: util-sort
license: sentieon:util=1
output file size: 2648863857
output reads: 67971401
bam_mem_sort: 63 calls 64.031 user 0.126 sys 64.251 real
bam_write: 63 calls 18.152 user 1.133 sys 19.578 real
execute: 1 calls 20.465 user 0.659 sys 402.391 real
merge_files: 1 calls 20.438 user 0.625 sys 22.790 real
parse_chunk: 1006 calls 91.640 user 16.083 sys 108.585 real
read_chunk: 2708 calls 34.201 user 22.242 sys 330.147 real
sort_block: 1 calls 25.137 user 21.253 sys 317.152 real
write_chunk: 1700 calls 19.821 user 0.443 sys 21.839 real
overall: 2598846464 mem 988.842 user 48.620 sys 402.413 real
ls: cannot access '*.cram': No such file or directory