.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T18-D0003255;L005;0004)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9c/5dd0737ec3efaef5147223e533fa61/.command.log
Size: 122.1 KB
Task: DAQ:SENTIEON_BWAMEM (T18-D0003255;L005;0004)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9c/5dd0737ec3efaef5147223e533fa61/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9f/5dc763eb84f01ca868fb7d0b1a7dc2/T18-D0003255/0004.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9f/5dc763eb84f01ca868fb7d0b1a7dc2/T18-D0003255/0004.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9c/5dd0737ec3efaef5147223e533fa61/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 2271.014 CPU sec, 624.968 real sec
[M::process] read 673474 sequences (100000005 bp) in 1.207 CPU sec, 1.211 real sec...
[M::process] read 673244 sequences (100000241 bp) in 1.649 CPU sec, 1.649 real sec...
[M::process] read 673330 sequences (100000133 bp) in 1.511 CPU sec, 1.525 real sec...
[M::process] read 673178 sequences (100000204 bp) in 1.404 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285287, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.59, 137.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 122, 172)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344)
[M::mem_pestat] mean and std.dev: (107.91, 47.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 430)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673244 reads in 114.961 CPU sec, 3.777 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285769, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.30, 134.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 18, 182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510)
[M::mem_pestat] mean and std.dev: (105.53, 138.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673360 sequences (100000188 bp) in 1.387 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285165, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.91, 136.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 76, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (167.36, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1423)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673474 reads in 365.617 CPU sec, 11.765 real sec
[M::mem_process_seqs] Processed 673330 reads in 235.805 CPU sec, 7.673 real sec
sorting block 0, #reads = 1079938 mem = 536879835 peak = 623181824
[M::process] read 673286 sequences (100000125 bp) in 1.685 CPU sec, 1.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285232, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.55, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 352, 9564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28656)
[M::mem_pestat] mean and std.dev: (2971.20, 4317.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38202)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673364 sequences (100000006 bp) in 1.369 CPU sec, 1.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285894, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.87, 135.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285165, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.39, 134.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 155, 1369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3925)
[M::mem_pestat] mean and std.dev: (438.40, 510.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5203)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673178 reads in 320.534 CPU sec, 10.319 real sec
sorting block 1, #reads = 1079673 mem = 536879734 peak = 899149824
[M::mem_process_seqs] Processed 673360 reads in 226.285 CPU sec, 7.364 real sec
[M::mem_process_seqs] Processed 673286 reads in 236.561 CPU sec, 7.726 real sec
sorting block 2, #reads = 1080118 mem = 536880685 peak = 957435904
[M::process] read 673396 sequences (100000200 bp) in 1.716 CPU sec, 1.813 real sec...
[M::process] read 673352 sequences (100000235 bp) in 1.695 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285087, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.09, 136.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 91, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113)
[M::mem_pestat] mean and std.dev: (102.11, 119.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1457)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673158 sequences (100000233 bp) in 1.388 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285438, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.22, 135.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673364 reads in 230.612 CPU sec, 7.409 real sec
[M::mem_process_seqs] Processed 673396 reads in 127.987 CPU sec, 4.118 real sec
sorting block 3, #reads = 1079920 mem = 536878249 peak = 957435904
sorting block 4, #reads = 1079618 mem = 536882462 peak = 957435904
[M::process] read 673186 sequences (100000117 bp) in 2.112 CPU sec, 2.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284973, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.06, 136.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 79, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (141.82, 186.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1439)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673096 sequences (100000013 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284291, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.66, 137.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 25, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (206.07, 321.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285059, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.72, 138.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 359, 1183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3505)
[M::mem_pestat] mean and std.dev: (650.38, 710.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4666)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673352 reads in 342.595 CPU sec, 11.006 real sec
[M::mem_process_seqs] Processed 673158 reads in 239.428 CPU sec, 7.744 real sec
[M::mem_process_seqs] Processed 673186 reads in 249.113 CPU sec, 8.085 real sec
sorting block 5, #reads = 1079794 mem = 536878068 peak = 957435904
[M::process] read 672598 sequences (100000269 bp) in 2.299 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284758, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.78, 141.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672896 sequences (100000104 bp) in 1.570 CPU sec, 1.597 real sec...
[M::process] read 672390 sequences (100000240 bp) in 1.440 CPU sec, 1.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284030, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 209, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (289.85, 154.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (350, 651, 746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538)
[M::mem_pestat] mean and std.dev: (387.22, 284.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1934)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673096 reads in 246.279 CPU sec, 7.869 real sec
sorting block 6, #reads = 1079490 mem = 536880115 peak = 957435904
[M::mem_process_seqs] Processed 672598 reads in 137.975 CPU sec, 4.434 real sec
sorting block 7, #reads = 1079382 mem = 536878159 peak = 957435904
[M::process] read 672748 sequences (100000083 bp) in 2.033 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284618, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.47, 144.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673294 sequences (100000250 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284166, 19, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.99, 148.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (40, 220, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873)
[M::mem_pestat] mean and std.dev: (212.20, 211.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2484)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283934, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 214, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (294.54, 158.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 119, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360)
[M::mem_pestat] mean and std.dev: (57.27, 52.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672896 reads in 349.886 CPU sec, 11.245 real sec
[M::mem_process_seqs] Processed 672748 reads in 244.541 CPU sec, 7.923 real sec
[M::mem_process_seqs] Processed 672390 reads in 255.599 CPU sec, 8.311 real sec
sorting block 8, #reads = 1079380 mem = 536879444 peak = 957435904
[M::process] read 673294 sequences (100000069 bp) in 2.407 CPU sec, 2.784 real sec...
sorting block 9, #reads = 1078895 mem = 536879056 peak = 957435904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285708, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.97, 139.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 35, 87)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215)
[M::mem_pestat] mean and std.dev: (37.80, 26.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673196 sequences (100000153 bp) in 1.602 CPU sec, 1.639 real sec...
[M::process] read 673284 sequences (100000050 bp) in 1.448 CPU sec, 1.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285469, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.14, 137.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 17, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250)
[M::mem_pestat] mean and std.dev: (26.69, 32.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 332)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673294 reads in 232.164 CPU sec, 7.437 real sec
[M::mem_process_seqs] Processed 673294 reads in 128.428 CPU sec, 4.107 real sec
sorting block 10, #reads = 1080024 mem = 536878175 peak = 957435904
[M::process] read 673354 sequences (100000076 bp) in 1.926 CPU sec, 2.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285339, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.13, 139.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673564 sequences (100000245 bp) in 1.310 CPU sec, 1.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285344, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.05, 138.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 50, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192)
[M::mem_pestat] mean and std.dev: (61.50, 42.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 245)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285287, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.24, 137.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 22, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (69.31, 73.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673196 reads in 333.156 CPU sec, 10.717 real sec
[M::mem_process_seqs] Processed 673284 reads in 229.873 CPU sec, 7.453 real sec
[M::mem_process_seqs] Processed 673354 reads in 240.633 CPU sec, 7.834 real sec
sorting block 11, #reads = 1079950 mem = 536876231 peak = 957435904
[M::process] read 673330 sequences (100000176 bp) in 2.418 CPU sec, 2.792 real sec...
sorting block 12, #reads = 1080197 mem = 536879077 peak = 957435904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285340, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.32, 134.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 303, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1687)
[M::mem_pestat] mean and std.dev: (346.08, 240.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2185)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673344 sequences (100000166 bp) in 1.462 CPU sec, 1.615 real sec...
[M::process] read 673316 sequences (100000216 bp) in 1.387 CPU sec, 1.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285142, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.03, 134.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673564 reads in 239.244 CPU sec, 7.654 real sec
[M::mem_process_seqs] Processed 673330 reads in 132.054 CPU sec, 4.236 real sec
sorting block 13, #reads = 1080025 mem = 536879716 peak = 957435904
[M::process] read 673268 sequences (100000015 bp) in 2.352 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285316, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.03, 136.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673186 sequences (100000048 bp) in 1.620 CPU sec, 1.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284771, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.40, 138.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (12, 13, 43)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 105)
[M::mem_pestat] mean and std.dev: (17.44, 14.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 136)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285462, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.04, 138.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673344 reads in 342.197 CPU sec, 10.985 real sec
[M::mem_process_seqs] Processed 673268 reads in 238.892 CPU sec, 7.716 real sec
[M::mem_process_seqs] Processed 673316 reads in 248.740 CPU sec, 8.064 real sec
sorting block 14, #reads = 1079913 mem = 536880736 peak = 957435904
sorting block 15, #reads = 1079922 mem = 536879795 peak = 957435904
[M::process] read 673134 sequences (100000061 bp) in 2.378 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284959, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.65, 139.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672636 sequences (100000100 bp) in 1.589 CPU sec, 1.652 real sec...
[M::process] read 672906 sequences (100000032 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284979, 16, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.73, 141.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 102, 794)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2356)
[M::mem_pestat] mean and std.dev: (179.38, 259.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3137)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673186 reads in 234.676 CPU sec, 7.516 real sec
[M::mem_process_seqs] Processed 673134 reads in 129.491 CPU sec, 4.148 real sec
sorting block 16, #reads = 1079899 mem = 536879774 peak = 957435904
[M::process] read 672576 sequences (100000250 bp) in 2.473 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284259, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 210, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (290.57, 155.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672754 sequences (100000077 bp) in 1.660 CPU sec, 1.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284178, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.36, 146.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 15, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (115.70, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284305, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (289.49, 154.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672636 reads in 340.353 CPU sec, 10.936 real sec
[M::mem_process_seqs] Processed 672906 reads in 235.253 CPU sec, 7.613 real sec
sorting block 17, #reads = 1079972 mem = 536877135 peak = 957435904
[M::mem_process_seqs] Processed 672576 reads in 245.263 CPU sec, 7.966 real sec
sorting block 18, #reads = 1078907 mem = 536877125 peak = 957435904
[M::process] read 673220 sequences (100000197 bp) in 2.115 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285222, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (283.58, 150.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (31, 98, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393)
[M::mem_pestat] mean and std.dev: (204.38, 219.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1847)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673336 sequences (100000115 bp) in 1.742 CPU sec, 1.923 real sec...
[M::process] read 673248 sequences (100000053 bp) in 1.130 CPU sec, 1.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285441, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.97, 143.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672754 reads in 222.452 CPU sec, 7.146 real sec
[M::mem_process_seqs] Processed 673220 reads in 122.035 CPU sec, 3.915 real sec
sorting block 19, #reads = 1078943 mem = 536877728 peak = 957435904
[M::process] read 673082 sequences (100000104 bp) in 2.281 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286179, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.47, 138.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673106 sequences (100000248 bp) in 1.599 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286273, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.91, 140.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 88, 885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2589)
[M::mem_pestat] mean and std.dev: (407.00, 462.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3441)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285821, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.70, 140.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673336 reads in 311.224 CPU sec, 9.979 real sec
[M::mem_process_seqs] Processed 673248 reads in 218.401 CPU sec, 7.045 real sec
sorting block 20, #reads = 1079508 mem = 536878499 peak = 957435904
[M::mem_process_seqs] Processed 673082 reads in 227.729 CPU sec, 7.372 real sec
sorting block 21, #reads = 1079938 mem = 536879922 peak = 996216832
[M::process] read 673380 sequences (100000244 bp) in 2.842 CPU sec, 3.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285458, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.62, 142.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673420 sequences (100000062 bp) in 1.794 CPU sec, 1.847 real sec...
[M::process] read 673350 sequences (100000234 bp) in 1.550 CPU sec, 1.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285445, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.31, 138.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673106 reads in 222.990 CPU sec, 7.125 real sec
[M::mem_process_seqs] Processed 673380 reads in 123.103 CPU sec, 3.914 real sec
sorting block 22, #reads = 1079777 mem = 536878487 peak = 996216832
[M::process] read 673246 sequences (100000097 bp) in 2.264 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285444, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.69, 136.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673144 sequences (100000015 bp) in 1.490 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285756, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.19, 137.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285610, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.52, 139.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 85, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222)
[M::mem_pestat] mean and std.dev: (58.27, 36.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 290)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673420 reads in 321.298 CPU sec, 10.305 real sec
sorting block 23, #reads = 1080307 mem = 536876626 peak = 996216832
[M::mem_process_seqs] Processed 673350 reads in 222.240 CPU sec, 7.183 real sec
[M::mem_process_seqs] Processed 673246 reads in 232.646 CPU sec, 7.550 real sec
sorting block 24, #reads = 1079940 mem = 536888046 peak = 996216832
[M::process] read 673250 sequences (100000250 bp) in 2.499 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285203, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.49, 140.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 122, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318)
[M::mem_pestat] mean and std.dev: (78.50, 59.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673044 sequences (100000222 bp) in 1.530 CPU sec, 1.600 real sec...
[M::process] read 673002 sequences (100000283 bp) in 1.150 CPU sec, 1.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285183, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.45, 141.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 25, 96)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282)
[M::mem_pestat] mean and std.dev: (30.00, 36.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 375)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673144 reads in 227.322 CPU sec, 7.292 real sec
[M::mem_process_seqs] Processed 673250 reads in 126.321 CPU sec, 4.062 real sec
sorting block 25, #reads = 1079964 mem = 536880356 peak = 996216832
sorting block 26, #reads = 1079980 mem = 536877820 peak = 1001631744
[M::process] read 672946 sequences (100000103 bp) in 2.106 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285604, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.22, 141.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 3, 33)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 93)
[M::mem_pestat] mean and std.dev: (11.86, 13.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 123)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672916 sequences (100000090 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284567, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.18, 143.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284628, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.80, 145.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673044 reads in 325.252 CPU sec, 10.448 real sec
[M::mem_process_seqs] Processed 673002 reads in 223.652 CPU sec, 7.239 real sec
[M::mem_process_seqs] Processed 672946 reads in 232.939 CPU sec, 7.566 real sec
sorting block 27, #reads = 1080170 mem = 536877082 peak = 1001631744
[M::process] read 672788 sequences (100000003 bp) in 2.673 CPU sec, 3.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284844, 16, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.68, 146.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 165, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550)
[M::mem_pestat] mean and std.dev: (224.56, 218.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2055)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672964 sequences (100000035 bp) in 1.450 CPU sec, 1.484 real sec...
[M::process] read 673198 sequences (100000246 bp) in 1.142 CPU sec, 1.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285161, 18, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.86, 148.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (85, 619, 662)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1816)
[M::mem_pestat] mean and std.dev: (339.79, 281.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2393)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672916 reads in 216.558 CPU sec, 6.954 real sec
sorting block 28, #reads = 1079646 mem = 536878747 peak = 1001631744
[M::mem_process_seqs] Processed 672788 reads in 121.633 CPU sec, 3.913 real sec
sorting block 29, #reads = 1079545 mem = 536879183 peak = 1015533568
[M::process] read 673238 sequences (100000214 bp) in 2.398 CPU sec, 2.598 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285454, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.33, 145.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673098 sequences (100000272 bp) in 1.180 CPU sec, 1.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285977, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.35, 136.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285856, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.93, 138.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672964 reads in 305.380 CPU sec, 9.820 real sec
[M::mem_process_seqs] Processed 673198 reads in 208.710 CPU sec, 6.751 real sec
[M::mem_process_seqs] Processed 673238 reads in 217.983 CPU sec, 7.079 real sec
sorting block 30, #reads = 1079783 mem = 536879275 peak = 1015533568
[M::process] read 673174 sequences (100000180 bp) in 2.485 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285401, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.52, 139.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1080363 mem = 536877253 peak = 1015533568
[M::process] read 673158 sequences (100000170 bp) in 2.168 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285558, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.16, 140.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 50, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198)
[M::mem_pestat] mean and std.dev: (53.73, 29.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 254)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673304 sequences (100000195 bp) in 1.690 CPU sec, 1.689 real sec...
[M::mem_process_seqs] Processed 673098 reads in 216.200 CPU sec, 6.895 real sec
[M::mem_process_seqs] Processed 673174 reads in 126.856 CPU sec, 4.033 real sec
sorting block 32, #reads = 1079411 mem = 536877815 peak = 1015533568
[M::process] read 673516 sequences (100000139 bp) in 2.507 CPU sec, 2.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285304, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (272.61, 140.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673198 sequences (100000264 bp) in 1.600 CPU sec, 1.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285653, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.80, 135.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285413, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.17, 136.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 226, 962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792)
[M::mem_pestat] mean and std.dev: (436.23, 402.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3707)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673158 reads in 314.778 CPU sec, 10.101 real sec
[M::mem_process_seqs] Processed 673304 reads in 216.715 CPU sec, 6.989 real sec
[M::mem_process_seqs] Processed 673516 reads in 225.905 CPU sec, 7.314 real sec
sorting block 33, #reads = 1079693 mem = 536879959 peak = 1015533568
[M::process] read 673162 sequences (100000123 bp) in 2.546 CPU sec, 2.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285496, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.88, 138.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 521, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771)
[M::mem_pestat] mean and std.dev: (451.92, 307.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2296)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 34, #reads = 1079964 mem = 536881874 peak = 1015533568
[M::process] read 673230 sequences (100000227 bp) in 1.744 CPU sec, 1.870 real sec...
[M::process] read 673106 sequences (100000004 bp) in 1.200 CPU sec, 1.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285503, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.44, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673198 reads in 209.428 CPU sec, 6.704 real sec
[M::mem_process_seqs] Processed 673162 reads in 118.426 CPU sec, 3.779 real sec
sorting block 35, #reads = 1080260 mem = 536878503 peak = 1015533568
[M::process] read 672958 sequences (100000081 bp) in 1.991 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285642, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.00, 139.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 15, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267)
[M::mem_pestat] mean and std.dev: (40.44, 57.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 355)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672966 sequences (100000082 bp) in 1.110 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285524, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.42, 142.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285525, 14, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.34, 141.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 73, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1925)
[M::mem_pestat] mean and std.dev: (237.38, 287.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2563)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673230 reads in 283.206 CPU sec, 9.101 real sec
[M::mem_process_seqs] Processed 672958 reads in 197.492 CPU sec, 6.388 real sec
[M::mem_process_seqs] Processed 673106 reads in 206.604 CPU sec, 6.710 real sec
sorting block 36, #reads = 1080262 mem = 536876887 peak = 1015533568
sorting block 37, #reads = 1079971 mem = 536879749 peak = 1015533568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284963, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.49, 143.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672948 sequences (100000162 bp) in 2.873 CPU sec, 3.313 real sec...
[M::mem_process_seqs] Processed 672966 reads in 104.991 CPU sec, 3.456 real sec
[M::process] read 672730 sequences (100000063 bp) in 1.716 CPU sec, 1.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285440, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (278.02, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672658 sequences (100000165 bp) in 1.560 CPU sec, 1.558 real sec...
[M::process] read 673478 sequences (100000160 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285206, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.46, 146.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (41, 148, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (83.62, 69.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672948 reads in 191.548 CPU sec, 6.066 real sec
sorting block 38, #reads = 1080058 mem = 536878423 peak = 1015533568
[M::mem_process_seqs] Processed 672730 reads in 107.919 CPU sec, 3.452 real sec
sorting block 39, #reads = 1080019 mem = 536878402 peak = 1029398528
[M::process] read 673458 sequences (100000009 bp) in 2.209 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285337, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (282.64, 147.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 448, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1777)
[M::mem_pestat] mean and std.dev: (331.00, 257.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2355)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673306 sequences (100000181 bp) in 1.160 CPU sec, 1.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284706, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.87, 140.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284651, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.69, 141.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 220, 1472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4156)
[M::mem_pestat] mean and std.dev: (613.91, 701.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5498)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672658 reads in 292.162 CPU sec, 9.388 real sec
[M::mem_process_seqs] Processed 673478 reads in 208.124 CPU sec, 6.720 real sec
[M::mem_process_seqs] Processed 673458 reads in 217.127 CPU sec, 7.039 real sec
sorting block 40, #reads = 1079483 mem = 536878544 peak = 1029398528
[M::process] read 673400 sequences (100000268 bp) in 2.099 CPU sec, 2.350 real sec...
sorting block 41, #reads = 1079781 mem = 536877837 peak = 1029398528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284461, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.81, 139.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673640 sequences (100000266 bp) in 1.987 CPU sec, 2.095 real sec...
[M::process] read 673422 sequences (100000108 bp) in 1.340 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284232, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.61, 141.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 44, 73)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213)
[M::mem_pestat] mean and std.dev: (34.00, 33.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673306 reads in 205.640 CPU sec, 6.560 real sec
[M::mem_process_seqs] Processed 673400 reads in 112.852 CPU sec, 3.597 real sec
sorting block 42, #reads = 1079987 mem = 536879780 peak = 1029398528
[M::process] read 673376 sequences (100000197 bp) in 1.837 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284552, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.74, 133.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673528 sequences (100000019 bp) in 1.210 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284202, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.91, 137.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284369, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.88, 138.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673640 reads in 290.290 CPU sec, 9.321 real sec
[M::mem_process_seqs] Processed 673376 reads in 202.805 CPU sec, 6.552 real sec
[M::mem_process_seqs] Processed 673422 reads in 211.775 CPU sec, 6.868 real sec
sorting block 43, #reads = 1079344 mem = 536881100 peak = 1029398528
[M::process] read 673296 sequences (100000071 bp) in 2.117 CPU sec, 2.418 real sec...
sorting block 44, #reads = 1079837 mem = 536878090 peak = 1029398528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284559, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.18, 135.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673260 sequences (100000079 bp) in 1.454 CPU sec, 1.521 real sec...
[M::process] read 673378 sequences (100000262 bp) in 1.470 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284277, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (264.15, 136.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673528 reads in 197.577 CPU sec, 6.325 real sec
[M::mem_process_seqs] Processed 673296 reads in 112.220 CPU sec, 3.582 real sec
sorting block 45, #reads = 1079467 mem = 536877423 peak = 1029398528
[M::process] read 673054 sequences (100000041 bp) in 1.891 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284617, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (265.12, 137.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (85, 130, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1642)
[M::mem_pestat] mean and std.dev: (334.73, 364.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2161)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672778 sequences (100000277 bp) in 1.120 CPU sec, 1.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284563, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.33, 138.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673260 reads in 193.200 CPU sec, 6.184 real sec
[M::mem_process_seqs] Processed 673378 reads in 109.528 CPU sec, 3.545 real sec
sorting block 46, #reads = 1079840 mem = 536877917 peak = 1029398528
[M::process] read 672988 sequences (100000209 bp) in 2.035 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283822, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.59, 143.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672718 sequences (100000021 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283763, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (283.73, 151.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284016, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.34, 147.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673054 reads in 293.989 CPU sec, 9.525 real sec
[M::mem_process_seqs] Processed 672778 reads in 204.795 CPU sec, 6.708 real sec
[M::mem_process_seqs] Processed 672988 reads in 211.449 CPU sec, 6.939 real sec
sorting block 47, #reads = 1079575 mem = 536881120 peak = 1029398528
sorting block 48, #reads = 1079622 mem = 536876695 peak = 1029398528
[M::process] read 673036 sequences (100000225 bp) in 2.303 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283633, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.15, 153.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 686, 1055)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2905)
[M::mem_pestat] mean and std.dev: (596.64, 429.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3830)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673578 sequences (100000103 bp) in 1.761 CPU sec, 1.895 real sec...
[M::process] read 673296 sequences (100000298 bp) in 1.180 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283998, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.55, 147.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672718 reads in 199.027 CPU sec, 6.379 real sec
[M::mem_process_seqs] Processed 673036 reads in 106.729 CPU sec, 3.392 real sec
sorting block 49, #reads = 1078312 mem = 536879797 peak = 1029398528
[M::process] read 673462 sequences (100000208 bp) in 2.030 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285080, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.43, 134.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673322 sequences (100000030 bp) in 1.630 CPU sec, 1.626 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284569, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.84, 136.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 224, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (159.25, 134.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285229, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.94, 137.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 86, 86)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172)
[M::mem_pestat] mean and std.dev: (64.45, 26.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 215)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673578 reads in 275.749 CPU sec, 8.825 real sec
sorting block 50, #reads = 1079313 mem = 536879838 peak = 1029398528
[M::mem_process_seqs] Processed 673296 reads in 189.154 CPU sec, 6.085 real sec
[M::mem_process_seqs] Processed 673462 reads in 196.208 CPU sec, 6.332 real sec
sorting block 51, #reads = 1079464 mem = 536878642 peak = 1029398528
[M::process] read 673530 sequences (100000154 bp) in 2.203 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284975, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.02, 133.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 38, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954)
[M::mem_pestat] mean and std.dev: (242.36, 327.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2597)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673436 sequences (100000291 bp) in 1.526 CPU sec, 1.589 real sec...
[M::process] read 673486 sequences (100000108 bp) in 1.230 CPU sec, 1.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285115, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.56, 131.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673322 reads in 185.531 CPU sec, 5.919 real sec
[M::mem_process_seqs] Processed 673530 reads in 100.953 CPU sec, 3.204 real sec
sorting block 52, #reads = 1079567 mem = 536877143 peak = 1029398528
sorting block 53, #reads = 1079695 mem = 536878619 peak = 1029398528
[M::process] read 673508 sequences (100000177 bp) in 1.999 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284659, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (262.65, 134.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 42, 171)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (64.50, 63.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673480 sequences (100000289 bp) in 1.220 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284589, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.33, 135.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284675, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.22, 135.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 209, 3694)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11040)
[M::mem_pestat] mean and std.dev: (1058.75, 1535.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14713)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673436 reads in 273.477 CPU sec, 8.765 real sec
[M::mem_process_seqs] Processed 673486 reads in 189.208 CPU sec, 6.087 real sec
[M::mem_process_seqs] Processed 673508 reads in 196.008 CPU sec, 6.325 real sec
sorting block 54, #reads = 1079676 mem = 536879744 peak = 1029398528
[M::process] read 673338 sequences (100000129 bp) in 2.053 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284703, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.32, 137.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 81, 81)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235)
[M::mem_pestat] mean and std.dev: (57.00, 51.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 312)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673330 sequences (100000282 bp) in 1.425 CPU sec, 1.491 real sec...
[M::process] read 672892 sequences (100000284 bp) in 1.190 CPU sec, 1.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284734, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.73, 137.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673480 reads in 188.976 CPU sec, 6.029 real sec
sorting block 55, #reads = 1079616 mem = 536879740 peak = 1029398528
[M::mem_process_seqs] Processed 673338 reads in 102.550 CPU sec, 3.252 real sec
sorting block 56, #reads = 1079825 mem = 536881958 peak = 1029398528
[M::process] read 673010 sequences (100000199 bp) in 1.865 CPU sec, 2.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284556, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.06, 140.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (5, 8, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081)
[M::mem_pestat] mean and std.dev: (314.45, 384.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2773)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673028 sequences (100000076 bp) in 1.200 CPU sec, 1.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283557, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (277.22, 148.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284087, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.89, 149.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673330 reads in 282.455 CPU sec, 9.056 real sec
[M::mem_process_seqs] Processed 672892 reads in 195.365 CPU sec, 6.295 real sec
[M::mem_process_seqs] Processed 673010 reads in 202.082 CPU sec, 6.529 real sec
sorting block 57, #reads = 1079995 mem = 536879804 peak = 1029398528
[M::process] read 673114 sequences (100000092 bp) in 2.154 CPU sec, 2.477 real sec...
sorting block 58, #reads = 1079410 mem = 536879841 peak = 1029398528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284242, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (278.00, 150.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (34, 94, 102)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238)
[M::mem_pestat] mean and std.dev: (94.70, 65.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 358)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673410 sequences (100000267 bp) in 1.302 CPU sec, 1.334 real sec...
[M::process] read 673366 sequences (100000009 bp) in 1.190 CPU sec, 1.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284621, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (277.31, 150.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 12, 887)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2653)
[M::mem_pestat] mean and std.dev: (488.78, 825.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3790)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673028 reads in 192.475 CPU sec, 6.142 real sec
[M::mem_process_seqs] Processed 673114 reads in 104.028 CPU sec, 3.311 real sec
sorting block 59, #reads = 1079650 mem = 536880133 peak = 1029398528
[M::process] read 673250 sequences (100000219 bp) in 1.950 CPU sec, 2.090 real sec...
[M::process] read 673274 sequences (100000235 bp) in 1.140 CPU sec, 1.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285227, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.27, 140.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673250 reads in 95.395 CPU sec, 3.054 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285145, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.39, 140.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 11, 71)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 201)
[M::mem_pestat] mean and std.dev: (23.58, 25.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 266)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673220 sequences (100000224 bp) in 1.458 CPU sec, 1.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285093, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.80, 138.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673410 reads in 276.160 CPU sec, 8.813 real sec
sorting block 60, #reads = 1080019 mem = 536877657 peak = 1029398528
[M::mem_process_seqs] Processed 673366 reads in 193.864 CPU sec, 6.200 real sec
sorting block 61, #reads = 1079624 mem = 536876959 peak = 1029398528
[M::process] read 673440 sequences (100000143 bp) in 1.635 CPU sec, 1.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284806, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.76, 139.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673500 sequences (100000218 bp) in 1.150 CPU sec, 1.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284492, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.43, 139.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284930, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 191, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (261.97, 133.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 40, 79)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187)
[M::mem_pestat] mean and std.dev: (33.25, 20.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 241)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673274 reads in 267.456 CPU sec, 8.580 real sec
[M::mem_process_seqs] Processed 673220 reads in 184.504 CPU sec, 5.949 real sec
[M::mem_process_seqs] Processed 673440 reads in 190.995 CPU sec, 6.176 real sec
sorting block 62, #reads = 1079329 mem = 536878188 peak = 1029398528
[M::process] read 673508 sequences (100000034 bp) in 2.333 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285083, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.62, 132.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1079847 mem = 536883092 peak = 1029398528
[M::process] read 673266 sequences (100000057 bp) in 1.570 CPU sec, 1.655 real sec...
[M::process] read 673428 sequences (100000189 bp) in 1.180 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284794, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (263.98, 136.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673500 reads in 189.380 CPU sec, 6.056 real sec
[M::mem_process_seqs] Processed 673508 reads in 104.270 CPU sec, 3.311 real sec
sorting block 64, #reads = 1080034 mem = 536881191 peak = 1029398528
[M::process] read 673288 sequences (100000276 bp) in 1.967 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284586, 15, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (265.70, 137.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 130, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2158)
[M::mem_pestat] mean and std.dev: (387.27, 484.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2876)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673238 sequences (100000113 bp) in 1.260 CPU sec, 1.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284638, 12, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.64, 140.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 94, 569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701)
[M::mem_pestat] mean and std.dev: (145.00, 215.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2267)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284276, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.29, 138.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673266 reads in 275.678 CPU sec, 8.836 real sec
[M::mem_process_seqs] Processed 673288 reads in 191.224 CPU sec, 6.156 real sec
[M::mem_process_seqs] Processed 673428 reads in 198.196 CPU sec, 6.400 real sec
sorting block 65, #reads = 1079556 mem = 536877934 peak = 1029398528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285063, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.81, 141.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1079985 mem = 536879197 peak = 1029398528
[M::mem_process_seqs] Processed 673238 reads in 103.381 CPU sec, 3.401 real sec
[M::process] read 673314 sequences (100000051 bp) in 3.119 CPU sec, 3.657 real sec...
[M::process] read 673218 sequences (100000285 bp) in 2.087 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284758, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.81, 145.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673220 sequences (100000291 bp) in 1.388 CPU sec, 1.499 real sec...
[M::process] read 673114 sequences (100000243 bp) in 1.500 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284868, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.60, 146.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673314 reads in 197.123 CPU sec, 6.249 real sec
sorting block 67, #reads = 1080177 mem = 536880119 peak = 1029398528
[M::mem_process_seqs] Processed 673218 reads in 110.292 CPU sec, 3.524 real sec
sorting block 68, #reads = 1079861 mem = 536877575 peak = 1029398528
[M::process] read 673764 sequences (100000212 bp) in 2.116 CPU sec, 2.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284685, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.05, 145.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673450 sequences (100000238 bp) in 1.210 CPU sec, 1.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285465, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.65, 138.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284817, 12, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.22, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 92, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225)
[M::mem_pestat] mean and std.dev: (192.67, 267.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1629)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673220 reads in 279.486 CPU sec, 8.969 real sec
[M::mem_process_seqs] Processed 673764 reads in 196.077 CPU sec, 6.331 real sec
[M::mem_process_seqs] Processed 673114 reads in 206.293 CPU sec, 6.694 real sec
sorting block 69, #reads = 1079764 mem = 536881420 peak = 1029398528
[M::process] read 673298 sequences (100000003 bp) in 2.050 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284660, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.35, 140.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 38, 775)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2313)
[M::mem_pestat] mean and std.dev: (370.18, 577.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3082)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673208 sequences (100000262 bp) in 1.349 CPU sec, 1.406 real sec...
[M::process] read 673170 sequences (100000177 bp) in 1.170 CPU sec, 1.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284892, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.24, 143.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673450 reads in 192.896 CPU sec, 6.183 real sec
sorting block 70, #reads = 1079386 mem = 536877917 peak = 1029398528
[M::mem_process_seqs] Processed 673298 reads in 109.697 CPU sec, 3.512 real sec
sorting block 71, #reads = 1079395 mem = 536879564 peak = 1029398528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284917, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (273.63, 143.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673614 sequences (100000126 bp) in 2.525 CPU sec, 2.811 real sec...
[M::process] read 673586 sequences (100000254 bp) in 1.189 CPU sec, 1.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284748, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.40, 142.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673208 reads in 192.255 CPU sec, 6.148 real sec
[M::mem_process_seqs] Processed 673170 reads in 110.112 CPU sec, 3.542 real sec
sorting block 72, #reads = 1079331 mem = 536878358 peak = 1029398528
[M::process] read 673468 sequences (100000232 bp) in 2.173 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285324, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.98, 131.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (6, 51, 148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432)
[M::mem_pestat] mean and std.dev: (51.89, 57.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 574)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673342 sequences (100000048 bp) in 1.220 CPU sec, 1.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285193, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.75, 134.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673614 reads in 186.140 CPU sec, 5.955 real sec
[M::mem_process_seqs] Processed 673586 reads in 106.749 CPU sec, 3.440 real sec
sorting block 73, #reads = 1079709 mem = 536877916 peak = 1029398528
[M::process] read 673450 sequences (100000105 bp) in 1.649 CPU sec, 1.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285631, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.42, 132.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673340 sequences (100000210 bp) in 1.200 CPU sec, 1.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284897, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.08, 138.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673468 reads in 185.165 CPU sec, 5.953 real sec
[M::mem_process_seqs] Processed 673342 reads in 104.889 CPU sec, 3.373 real sec
sorting block 74, #reads = 1079555 mem = 536877642 peak = 1029398528
[M::process] read 673146 sequences (100000130 bp) in 1.646 CPU sec, 1.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284995, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (269.11, 140.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673120 sequences (100000077 bp) in 1.200 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284817, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (273.88, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284809, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.84, 141.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 217, 738)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210)
[M::mem_pestat] mean and std.dev: (204.90, 277.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2946)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673450 reads in 273.753 CPU sec, 8.793 real sec
sorting block 75, #reads = 1079827 mem = 536877905 peak = 1029398528
[M::mem_process_seqs] Processed 673146 reads in 195.561 CPU sec, 6.332 real sec
[M::mem_process_seqs] Processed 673340 reads in 205.988 CPU sec, 6.703 real sec
sorting block 76, #reads = 1080145 mem = 536877095 peak = 1029398528
[M::process] read 673094 sequences (100000272 bp) in 2.444 CPU sec, 2.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284654, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.48, 146.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673070 sequences (100000236 bp) in 2.312 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284918, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (276.49, 147.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673120 reads in 197.268 CPU sec, 6.376 real sec
[M::process] read 483614 sequences (71853166 bp) in 1.184 CPU sec, 1.197 real sec...
sorting block 77, #reads = 1080078 mem = 536879306 peak = 1029398528
[M::mem_process_seqs] Processed 673094 reads in 115.002 CPU sec, 3.671 real sec
sorting block 78, #reads = 1079747 mem = 536877443 peak = 1031946240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284341, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (278.61, 150.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 204919, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (279.50, 152.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673070 reads in 160.431 CPU sec, 5.217 real sec
[M::mem_process_seqs] Processed 483614 reads in 78.979 CPU sec, 2.574 real sec
sorting block 79, #reads = 1079465 mem = 536876662 peak = 1031946240
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 85982290
overall: 69765730304 mem 15288.885 user 758.499 sys 1081.797 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L005\tPU:L005\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.T18-D0003255_R1.fastq.gz 0004.T18-D0003255_R2.fastq.gz
[main] Real time: 1081.827 sec; CPU: 16047.388 sec
sorting block 80, #reads = 225822 mem = 112442144 peak = 1031946240
uniq header count 1
merging 81 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3302241536
output reads: 86604634
bam_mem_sort: 81 calls 52.408 user 1.824 sys 54.379 real
bam_write: 81 calls 29.389 user 1.973 sys 31.448 real
execute: 1 calls 20.178 user 1.736 sys 1105.032 real
merge_files: 1 calls 20.156 user 1.705 sys 22.903 real
parse_chunk: 1284 calls 54.500 user 14.383 sys 69.945 real
read_chunk: 3456 calls 31.055 user 25.756 sys 459.314 real
sort_block: 1 calls 23.370 user 25.000 sys 447.711 real
write_chunk: 2170 calls 19.768 user 1.439 sys 21.642 real
overall: 2302091264 mem 807.006 user 61.131 sys 1105.049 real
ls: cannot access '*.cram': No such file or directory