.command.log - Workdir - DAQ:SENTIEON_BWAMEM (T18-D0003255;L007;0003)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/31/968a01e022a80f788b0b1484c1dd65/.command.log
Size: 120.5 KB
Task: DAQ:SENTIEON_BWAMEM (T18-D0003255;L007;0003)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/44/88c1ff3d84367dbde1783f65027ec9/T18-D0003255/0003.T18-D0003255_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/31/968a01e022a80f788b0b1484c1dd65/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/44/88c1ff3d84367dbde1783f65027ec9/T18-D0003255/0003.T18-D0003255_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/31/968a01e022a80f788b0b1484c1dd65/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o T18-D0003255.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1760.495 CPU sec, 307.659 real sec
[M::process] read 674118 sequences (100000190 bp) in 0.893 CPU sec, 0.897 real sec...
[M::process] read 673974 sequences (100000056 bp) in 1.100 CPU sec, 1.106 real sec...
[M::process] read 673778 sequences (100000067 bp) in 1.069 CPU sec, 1.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286648, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (249.58, 121.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 69, 122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272)
[M::mem_pestat] mean and std.dev: (65.00, 34.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 347)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673584 sequences (100000094 bp) in 1.010 CPU sec, 1.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286258, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (253.24, 124.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 86, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (100.60, 100.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286048, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (255.23, 126.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674118 reads in 250.681 CPU sec, 8.041 real sec
[M::mem_process_seqs] Processed 673974 reads in 164.715 CPU sec, 5.355 real sec
[M::mem_process_seqs] Processed 673778 reads in 171.953 CPU sec, 5.610 real sec
sorting block 0, #reads = 1080360 mem = 536877660 peak = 614248448
[M::process] read 673622 sequences (100000020 bp) in 1.420 CPU sec, 1.529 real sec...
[M::process] read 673480 sequences (100000233 bp) in 0.948 CPU sec, 0.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285654, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.29, 127.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673350 sequences (100000290 bp) in 0.963 CPU sec, 0.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285649, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (258.91, 129.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 122, 180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344)
[M::mem_pestat] mean and std.dev: (101.14, 50.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285611, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (260.15, 130.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673584 reads in 243.573 CPU sec, 7.819 real sec
sorting block 1, #reads = 1080015 mem = 536880358 peak = 851750912
[M::mem_process_seqs] Processed 673622 reads in 170.901 CPU sec, 5.518 real sec
[M::mem_process_seqs] Processed 673480 reads in 178.205 CPU sec, 5.773 real sec
sorting block 2, #reads = 1080147 mem = 536881159 peak = 877842432
[M::process] read 673370 sequences (100000236 bp) in 2.039 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285439, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (262.15, 131.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673244 sequences (100000168 bp) in 1.021 CPU sec, 1.026 real sec...
[M::process] read 673114 sequences (100000018 bp) in 0.950 CPU sec, 0.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285254, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.77, 133.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 29, 91)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225)
[M::mem_pestat] mean and std.dev: (31.89, 24.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 292)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673350 reads in 172.641 CPU sec, 5.530 real sec
[M::mem_process_seqs] Processed 673370 reads in 96.231 CPU sec, 3.085 real sec
sorting block 3, #reads = 1079621 mem = 536878979 peak = 900763648
sorting block 4, #reads = 1079969 mem = 536881385 peak = 919273472
[M::process] read 673168 sequences (100000000 bp) in 1.613 CPU sec, 1.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284518, 16, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.59, 135.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 210, 792)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328)
[M::mem_pestat] mean and std.dev: (404.00, 379.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3096)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673024 sequences (100000182 bp) in 1.089 CPU sec, 1.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285035, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (267.42, 136.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284962, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (270.21, 138.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 33, 1422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4220)
[M::mem_pestat] mean and std.dev: (477.36, 629.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5619)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673244 reads in 284.461 CPU sec, 9.143 real sec
[M::mem_process_seqs] Processed 673114 reads in 200.350 CPU sec, 6.500 real sec
[M::mem_process_seqs] Processed 673168 reads in 207.984 CPU sec, 6.768 real sec
sorting block 5, #reads = 1079481 mem = 536879735 peak = 919273472
[M::process] read 672990 sequences (100000201 bp) in 1.438 CPU sec, 1.582 real sec...
[M::process] read 672966 sequences (100000034 bp) in 1.357 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285080, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (271.23, 138.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672680 sequences (100000189 bp) in 1.000 CPU sec, 1.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285177, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.45, 142.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 221, 1017)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2847)
[M::mem_pestat] mean and std.dev: (564.00, 682.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3762)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284782, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.91, 142.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673024 reads in 288.596 CPU sec, 9.290 real sec
sorting block 6, #reads = 1079737 mem = 536878308 peak = 919273472
[M::mem_process_seqs] Processed 672990 reads in 198.610 CPU sec, 6.426 real sec
[M::mem_process_seqs] Processed 672966 reads in 206.224 CPU sec, 6.695 real sec
sorting block 7, #reads = 1080260 mem = 536879928 peak = 975982592
[M::process] read 672726 sequences (100000273 bp) in 1.932 CPU sec, 2.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284167, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 201, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (279.69, 146.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (65, 89, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2072)
[M::mem_pestat] mean and std.dev: (197.33, 277.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2741)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673706 sequences (100000257 bp) in 1.722 CPU sec, 1.808 real sec...
[M::process] read 673822 sequences (100000269 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285157, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 202, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (282.33, 149.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 68, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168)
[M::mem_pestat] mean and std.dev: (107.70, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1552)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672680 reads in 201.146 CPU sec, 6.439 real sec
[M::mem_process_seqs] Processed 672726 reads in 108.326 CPU sec, 3.461 real sec
sorting block 8, #reads = 1079678 mem = 536877386 peak = 975982592
sorting block 9, #reads = 1079597 mem = 536877646 peak = 975982592
[M::process] read 673686 sequences (100000011 bp) in 1.697 CPU sec, 1.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286243, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.57, 131.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (19, 655, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2053)
[M::mem_pestat] mean and std.dev: (443.50, 369.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2731)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673722 sequences (100000027 bp) in 1.140 CPU sec, 1.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285957, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.94, 125.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286266, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.93, 127.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673706 reads in 274.975 CPU sec, 8.869 real sec
[M::mem_process_seqs] Processed 673822 reads in 190.925 CPU sec, 6.248 real sec
[M::mem_process_seqs] Processed 673686 reads in 199.097 CPU sec, 6.537 real sec
sorting block 10, #reads = 1079815 mem = 536879729 peak = 975982592
[M::process] read 673576 sequences (100000193 bp) in 1.876 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286384, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.51, 128.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673542 sequences (100000220 bp) in 1.662 CPU sec, 1.734 real sec...
[M::process] read 673208 sequences (100000033 bp) in 1.058 CPU sec, 1.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286129, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (259.85, 130.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673722 reads in 190.469 CPU sec, 6.111 real sec
sorting block 11, #reads = 1080188 mem = 536876878 peak = 975982592
[M::mem_process_seqs] Processed 673576 reads in 102.690 CPU sec, 3.283 real sec
sorting block 12, #reads = 1080539 mem = 536876166 peak = 975982592
[M::process] read 673336 sequences (100000184 bp) in 1.976 CPU sec, 2.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285654, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.86, 131.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 25, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370)
[M::mem_pestat] mean and std.dev: (31.22, 40.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673230 sequences (100000292 bp) in 1.050 CPU sec, 1.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286032, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.28, 133.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285115, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.60, 136.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673542 reads in 268.495 CPU sec, 8.618 real sec
[M::mem_process_seqs] Processed 673208 reads in 184.709 CPU sec, 5.957 real sec
[M::mem_process_seqs] Processed 673336 reads in 192.100 CPU sec, 6.216 real sec
sorting block 13, #reads = 1080397 mem = 536879459 peak = 975982592
[M::process] read 673320 sequences (100000064 bp) in 2.090 CPU sec, 2.252 real sec...
sorting block 14, #reads = 1080258 mem = 536877826 peak = 975982592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285190, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.67, 137.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 188, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1627)
[M::mem_pestat] mean and std.dev: (238.00, 257.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2161)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673172 sequences (100000227 bp) in 1.383 CPU sec, 1.390 real sec...
[M::process] read 673136 sequences (100000007 bp) in 1.270 CPU sec, 1.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285615, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.65, 137.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673230 reads in 188.684 CPU sec, 6.022 real sec
[M::mem_process_seqs] Processed 673320 reads in 104.452 CPU sec, 3.331 real sec
sorting block 15, #reads = 1079884 mem = 536880015 peak = 975982592
[M::process] read 672920 sequences (100000091 bp) in 1.788 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285224, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (269.48, 137.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673076 sequences (100000243 bp) in 1.080 CPU sec, 1.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284989, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.51, 138.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284760, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.29, 140.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673172 reads in 267.966 CPU sec, 8.608 real sec
[M::mem_process_seqs] Processed 673136 reads in 185.502 CPU sec, 5.998 real sec
[M::mem_process_seqs] Processed 672920 reads in 193.023 CPU sec, 6.260 real sec
sorting block 16, #reads = 1080398 mem = 536877629 peak = 975982592
sorting block 17, #reads = 1079845 mem = 536878556 peak = 975982592
[M::process] read 672816 sequences (100000235 bp) in 2.276 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284702, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.07, 142.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672792 sequences (100000188 bp) in 1.290 CPU sec, 1.301 real sec...
[M::process] read 673220 sequences (100000035 bp) in 1.100 CPU sec, 1.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285061, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.48, 146.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 44, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (174.75, 234.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1353)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673076 reads in 190.381 CPU sec, 6.075 real sec
[M::mem_process_seqs] Processed 672816 reads in 104.586 CPU sec, 3.336 real sec
sorting block 18, #reads = 1079700 mem = 536879889 peak = 975982592
[M::process] read 673940 sequences (100000256 bp) in 2.004 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284694, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.86, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 220, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (223.40, 264.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673882 sequences (100000276 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285647, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.52, 140.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 49, 164)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444)
[M::mem_pestat] mean and std.dev: (50.44, 54.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286542, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.01, 125.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 65, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354)
[M::mem_pestat] mean and std.dev: (43.50, 40.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672792 reads in 263.952 CPU sec, 8.451 real sec
[M::mem_process_seqs] Processed 673220 reads in 180.274 CPU sec, 5.809 real sec
[M::mem_process_seqs] Processed 673940 reads in 187.843 CPU sec, 6.074 real sec
sorting block 19, #reads = 1079237 mem = 536880111 peak = 975982592
sorting block 20, #reads = 1079855 mem = 536878020 peak = 975982592
[M::process] read 673708 sequences (100000057 bp) in 1.725 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286435, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.72, 127.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673754 sequences (100000054 bp) in 1.631 CPU sec, 1.695 real sec...
[M::process] read 673734 sequences (100000279 bp) in 1.440 CPU sec, 1.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286243, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.02, 131.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673882 reads in 182.101 CPU sec, 5.800 real sec
[M::mem_process_seqs] Processed 673708 reads in 101.303 CPU sec, 3.220 real sec
sorting block 21, #reads = 1080876 mem = 536878052 peak = 975982592
[M::process] read 673486 sequences (100000093 bp) in 1.806 CPU sec, 1.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285882, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.16, 130.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673292 sequences (100000211 bp) in 1.440 CPU sec, 1.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286025, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.52, 131.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285722, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (262.24, 133.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673754 reads in 265.870 CPU sec, 8.519 real sec
[M::mem_process_seqs] Processed 673734 reads in 185.015 CPU sec, 5.959 real sec
sorting block 22, #reads = 1080377 mem = 536879638 peak = 975982592
[M::mem_process_seqs] Processed 673486 reads in 192.465 CPU sec, 6.219 real sec
sorting block 23, #reads = 1080350 mem = 536882614 peak = 975982592
[M::process] read 673236 sequences (100000299 bp) in 2.281 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285226, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (267.39, 136.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673214 sequences (100000013 bp) in 1.802 CPU sec, 1.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285575, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.39, 138.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673106 sequences (100000265 bp) in 1.530 CPU sec, 1.537 real sec...
[M::mem_process_seqs] Processed 673292 reads in 187.231 CPU sec, 5.961 real sec
[M::mem_process_seqs] Processed 673236 reads in 102.823 CPU sec, 3.251 real sec
sorting block 24, #reads = 1080244 mem = 536879567 peak = 975982592
[M::process] read 672972 sequences (100000209 bp) in 1.934 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285370, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.61, 140.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 79, 4340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12988)
[M::mem_pestat] mean and std.dev: (1459.77, 2527.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17312)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672896 sequences (100000147 bp) in 1.290 CPU sec, 1.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285107, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.84, 141.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 30, 98)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288)
[M::mem_pestat] mean and std.dev: (22.60, 27.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 383)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285219, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (273.59, 142.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 194, 2055)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5901)
[M::mem_pestat] mean and std.dev: (641.64, 868.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7824)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673214 reads in 269.500 CPU sec, 8.634 real sec
[M::mem_process_seqs] Processed 673106 reads in 187.498 CPU sec, 6.027 real sec
sorting block 25, #reads = 1080132 mem = 536881525 peak = 975982592
[M::mem_process_seqs] Processed 672972 reads in 194.484 CPU sec, 6.266 real sec
sorting block 26, #reads = 1079952 mem = 536878068 peak = 975982592
[M::process] read 672920 sequences (100000106 bp) in 2.186 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284641, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (275.62, 143.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (10, 29, 1000)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2980)
[M::mem_pestat] mean and std.dev: (339.22, 625.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3970)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672870 sequences (100000094 bp) in 1.390 CPU sec, 1.422 real sec...
[M::process] read 672802 sequences (100000078 bp) in 1.210 CPU sec, 1.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285185, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.19, 143.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672896 reads in 189.139 CPU sec, 6.034 real sec
[M::mem_process_seqs] Processed 672920 reads in 101.604 CPU sec, 3.235 real sec
sorting block 27, #reads = 1079352 mem = 536878478 peak = 975982592
[M::process] read 672918 sequences (100000041 bp) in 2.073 CPU sec, 2.211 real sec...
[M::process] read 673886 sequences (100000149 bp) in 1.470 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285187, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.51, 148.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 376, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (233.30, 176.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1417)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672802 reads in 97.782 CPU sec, 3.120 real sec
sorting block 28, #reads = 1079525 mem = 536879793 peak = 975982592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284724, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.52, 145.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673858 sequences (100000021 bp) in 1.376 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285447, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.30, 144.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672870 reads in 279.282 CPU sec, 8.900 real sec
[M::mem_process_seqs] Processed 672918 reads in 195.291 CPU sec, 6.234 real sec
sorting block 29, #reads = 1079625 mem = 536879847 peak = 1016193024
[M::process] read 673656 sequences (100000083 bp) in 1.950 CPU sec, 2.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286054, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (251.68, 122.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673756 sequences (100000222 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286281, 17, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.03, 125.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (35, 120, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (112.47, 90.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285991, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.35, 127.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673886 reads in 259.112 CPU sec, 8.295 real sec
[M::mem_process_seqs] Processed 673858 reads in 179.468 CPU sec, 5.759 real sec
sorting block 30, #reads = 1079204 mem = 536878187 peak = 1016193024
[M::mem_process_seqs] Processed 673656 reads in 186.477 CPU sec, 6.011 real sec
sorting block 31, #reads = 1080418 mem = 536879785 peak = 1016193024
[M::process] read 673602 sequences (100000183 bp) in 2.397 CPU sec, 2.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285519, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (257.35, 127.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673424 sequences (100000265 bp) in 1.511 CPU sec, 1.622 real sec...
[M::process] read 673330 sequences (100000171 bp) in 1.160 CPU sec, 1.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285259, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.19, 129.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673756 reads in 184.258 CPU sec, 5.868 real sec
[M::mem_process_seqs] Processed 673602 reads in 100.319 CPU sec, 3.178 real sec
sorting block 32, #reads = 1080132 mem = 536880781 peak = 1016193024
[M::process] read 673346 sequences (100000141 bp) in 1.731 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285920, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (261.40, 130.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673134 sequences (100000063 bp) in 1.140 CPU sec, 1.139 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284943, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (264.30, 133.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 161, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (220.91, 140.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285583, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (266.36, 135.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673424 reads in 270.295 CPU sec, 8.669 real sec
sorting block 33, #reads = 1080163 mem = 536876898 peak = 1016193024
[M::mem_process_seqs] Processed 673330 reads in 185.784 CPU sec, 5.987 real sec
[M::mem_process_seqs] Processed 673346 reads in 192.510 CPU sec, 6.221 real sec
sorting block 34, #reads = 1080175 mem = 536882624 peak = 1027334144
[M::process] read 673078 sequences (100000094 bp) in 2.188 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285487, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (268.46, 137.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (75, 93, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1941)
[M::mem_pestat] mean and std.dev: (194.30, 218.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2563)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672962 sequences (100000040 bp) in 1.779 CPU sec, 1.855 real sec...
[M::process] read 672956 sequences (100000179 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285403, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.97, 139.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (71, 86, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721)
[M::mem_pestat] mean and std.dev: (183.91, 217.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2271)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673134 reads in 186.906 CPU sec, 5.953 real sec
[M::mem_process_seqs] Processed 673078 reads in 103.282 CPU sec, 3.266 real sec
sorting block 35, #reads = 1080049 mem = 536878671 peak = 1027334144
sorting block 36, #reads = 1080125 mem = 536879416 peak = 1027334144
[M::process] read 672908 sequences (100000008 bp) in 1.665 CPU sec, 1.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284688, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (273.08, 140.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (43, 50, 75)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139)
[M::mem_pestat] mean and std.dev: (50.88, 15.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 171)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672802 sequences (100000143 bp) in 1.190 CPU sec, 1.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285232, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (273.46, 141.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284658, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.28, 144.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (38, 1014, 1014)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2966)
[M::mem_pestat] mean and std.dev: (428.90, 478.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3942)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672962 reads in 268.124 CPU sec, 8.600 real sec
[M::mem_process_seqs] Processed 672956 reads in 184.142 CPU sec, 5.937 real sec
[M::mem_process_seqs] Processed 672908 reads in 191.048 CPU sec, 6.174 real sec
sorting block 37, #reads = 1080492 mem = 536879102 peak = 1027334144
[M::process] read 672804 sequences (100000011 bp) in 2.281 CPU sec, 2.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284969, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 200, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (277.54, 144.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672652 sequences (100000204 bp) in 1.220 CPU sec, 1.220 real sec...
[M::process] read 673650 sequences (100000071 bp) in 1.270 CPU sec, 1.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284618, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 203, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (280.06, 145.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672802 reads in 186.034 CPU sec, 5.916 real sec
sorting block 38, #reads = 1079047 mem = 536878375 peak = 1027334144
[M::mem_process_seqs] Processed 672804 reads in 100.140 CPU sec, 3.181 real sec
sorting block 39, #reads = 1079402 mem = 536877317 peak = 1065373696
[M::process] read 674028 sequences (100000169 bp) in 2.011 CPU sec, 2.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285802, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 204, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (282.98, 148.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674050 sequences (100000064 bp) in 1.133 CPU sec, 1.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285754, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (259.00, 131.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672652 reads in 168.653 CPU sec, 5.381 real sec
[M::mem_process_seqs] Processed 673650 reads in 92.887 CPU sec, 2.972 real sec
sorting block 40, #reads = 1078966 mem = 536877443 peak = 1065373696
[M::process] read 673976 sequences (100000123 bp) in 1.997 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286123, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (250.22, 124.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673858 sequences (100000162 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285017, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.36, 125.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285212, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (254.39, 128.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674028 reads in 248.390 CPU sec, 7.945 real sec
[M::mem_process_seqs] Processed 674050 reads in 174.257 CPU sec, 5.602 real sec
[M::mem_process_seqs] Processed 673976 reads in 180.568 CPU sec, 5.821 real sec
sorting block 41, #reads = 1080452 mem = 536878602 peak = 1065373696
sorting block 42, #reads = 1080310 mem = 536878422 peak = 1065373696
[M::process] read 673752 sequences (100000161 bp) in 1.713 CPU sec, 1.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285116, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.68, 129.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673634 sequences (100000232 bp) in 1.383 CPU sec, 1.431 real sec...
[M::process] read 673506 sequences (100000173 bp) in 1.090 CPU sec, 1.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285072, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.26, 129.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673858 reads in 175.877 CPU sec, 5.614 real sec
[M::mem_process_seqs] Processed 673752 reads in 95.341 CPU sec, 3.034 real sec
sorting block 43, #reads = 1079755 mem = 536878989 peak = 1065373696
[M::process] read 673518 sequences (100000099 bp) in 1.755 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284854, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.42, 133.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673352 sequences (100000240 bp) in 1.090 CPU sec, 1.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284212, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (263.67, 136.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 92, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209)
[M::mem_pestat] mean and std.dev: (138.00, 155.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1601)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284434, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 192, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.80, 137.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673634 reads in 258.391 CPU sec, 8.283 real sec
[M::mem_process_seqs] Processed 673506 reads in 178.707 CPU sec, 5.764 real sec
[M::mem_process_seqs] Processed 673518 reads in 185.306 CPU sec, 5.994 real sec
sorting block 44, #reads = 1079835 mem = 536879498 peak = 1065373696
sorting block 45, #reads = 1079724 mem = 536879502 peak = 1065373696
[M::process] read 673204 sequences (100000098 bp) in 2.100 CPU sec, 2.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284201, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.21, 138.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673256 sequences (100000101 bp) in 1.239 CPU sec, 1.257 real sec...
[M::process] read 673170 sequences (100000231 bp) in 1.120 CPU sec, 1.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284477, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.27, 141.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 24, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (46.73, 59.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673352 reads in 182.480 CPU sec, 5.819 real sec
[M::mem_process_seqs] Processed 673204 reads in 99.801 CPU sec, 3.176 real sec
sorting block 46, #reads = 1080030 mem = 536878852 peak = 1065373696
[M::process] read 673160 sequences (100000150 bp) in 1.592 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284142, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.32, 141.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672932 sequences (100000197 bp) in 1.110 CPU sec, 1.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284211, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.31, 142.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 233, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2043)
[M::mem_pestat] mean and std.dev: (334.17, 331.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2716)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673256 reads in 176.617 CPU sec, 5.649 real sec
[M::mem_process_seqs] Processed 673170 reads in 95.640 CPU sec, 3.081 real sec
sorting block 47, #reads = 1079570 mem = 536877060 peak = 1065373696
[M::process] read 673228 sequences (100000276 bp) in 1.626 CPU sec, 1.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284490, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (271.48, 142.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674082 sequences (100000011 bp) in 1.250 CPU sec, 1.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284340, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (277.33, 148.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284259, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.22, 148.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 199, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (286.27, 228.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673160 reads in 259.359 CPU sec, 8.311 real sec
sorting block 48, #reads = 1079321 mem = 536878644 peak = 1065373696
[M::mem_process_seqs] Processed 672932 reads in 178.518 CPU sec, 5.758 real sec
[M::mem_process_seqs] Processed 673228 reads in 185.234 CPU sec, 5.990 real sec
sorting block 49, #reads = 1079367 mem = 536878117 peak = 1099313152
[M::process] read 674084 sequences (100000065 bp) in 1.825 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286146, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (247.79, 122.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673924 sequences (100000283 bp) in 1.473 CPU sec, 1.517 real sec...
[M::process] read 673892 sequences (100000082 bp) in 1.090 CPU sec, 1.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286254, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (249.97, 125.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (67, 92, 871)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2479)
[M::mem_pestat] mean and std.dev: (280.73, 449.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3283)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674082 reads in 166.510 CPU sec, 5.314 real sec
sorting block 50, #reads = 1079553 mem = 536879968 peak = 1099313152
[M::mem_process_seqs] Processed 674084 reads in 91.252 CPU sec, 2.896 real sec
sorting block 51, #reads = 1080522 mem = 536880752 peak = 1099313152
[M::process] read 673768 sequences (100000029 bp) in 1.976 CPU sec, 2.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286224, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.25, 127.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673610 sequences (100000115 bp) in 1.110 CPU sec, 1.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285842, 17, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.23, 129.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (24, 78, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116)
[M::mem_pestat] mean and std.dev: (186.81, 240.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1480)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285497, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (256.62, 130.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673924 reads in 248.296 CPU sec, 7.952 real sec
[M::mem_process_seqs] Processed 673892 reads in 172.801 CPU sec, 5.549 real sec
[M::mem_process_seqs] Processed 673768 reads in 179.616 CPU sec, 5.786 real sec
sorting block 52, #reads = 1080660 mem = 536878798 peak = 1099313152
[M::process] read 673588 sequences (100000225 bp) in 1.730 CPU sec, 1.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285278, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (258.51, 131.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1079606 mem = 536877237 peak = 1099313152
[M::process] read 673470 sequences (100000122 bp) in 1.689 CPU sec, 1.775 real sec...
[M::process] read 673418 sequences (100000201 bp) in 1.140 CPU sec, 1.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284845, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.08, 137.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673610 reads in 174.664 CPU sec, 5.566 real sec
[M::mem_process_seqs] Processed 673588 reads in 97.057 CPU sec, 3.078 real sec
sorting block 54, #reads = 1079756 mem = 536879140 peak = 1099313152
[M::process] read 673366 sequences (100000050 bp) in 1.719 CPU sec, 1.836 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284541, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (264.05, 137.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673344 sequences (100000041 bp) in 1.130 CPU sec, 1.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284971, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (267.22, 140.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284274, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (268.28, 141.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (20, 41, 94)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242)
[M::mem_pestat] mean and std.dev: (47.22, 33.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 316)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673470 reads in 257.336 CPU sec, 8.252 real sec
[M::mem_process_seqs] Processed 673418 reads in 178.421 CPU sec, 5.749 real sec
[M::mem_process_seqs] Processed 673366 reads in 185.100 CPU sec, 5.981 real sec
sorting block 55, #reads = 1080227 mem = 536879169 peak = 1099313152
[M::process] read 673166 sequences (100000028 bp) in 1.757 CPU sec, 1.949 real sec...
sorting block 56, #reads = 1079880 mem = 536881098 peak = 1099313152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284276, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.06, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 22, 24)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 70)
[M::mem_pestat] mean and std.dev: (10.75, 9.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 93)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673224 sequences (100000187 bp) in 1.358 CPU sec, 1.410 real sec...
[M::process] read 673154 sequences (100000260 bp) in 1.120 CPU sec, 1.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284644, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (272.91, 144.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 39, 758)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2248)
[M::mem_pestat] mean and std.dev: (231.64, 323.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2993)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673344 reads in 178.091 CPU sec, 5.685 real sec
[M::mem_process_seqs] Processed 673166 reads in 96.315 CPU sec, 3.065 real sec
sorting block 57, #reads = 1079745 mem = 536880132 peak = 1099313152
[M::process] read 673162 sequences (100000261 bp) in 1.672 CPU sec, 1.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284440, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.23, 144.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673794 sequences (100000263 bp) in 1.120 CPU sec, 1.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284759, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (275.26, 148.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284988, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.32, 150.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (384, 1049, 1509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3759)
[M::mem_pestat] mean and std.dev: (848.80, 560.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4884)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673224 reads in 251.468 CPU sec, 8.063 real sec
[M::mem_process_seqs] Processed 673154 reads in 172.800 CPU sec, 5.566 real sec
[M::mem_process_seqs] Processed 673162 reads in 179.390 CPU sec, 5.795 real sec
sorting block 58, #reads = 1080062 mem = 536879135 peak = 1099313152
sorting block 59, #reads = 1080232 mem = 536880626 peak = 1099313152
[M::process] read 674046 sequences (100000091 bp) in 1.842 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285272, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (257.41, 132.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2, 3, 130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (18.10, 37.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673952 sequences (100000243 bp) in 1.507 CPU sec, 1.558 real sec...
[M::process] read 673914 sequences (100000055 bp) in 1.110 CPU sec, 1.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285994, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.22, 126.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 559, 1841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5133)
[M::mem_pestat] mean and std.dev: (739.40, 740.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6779)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673794 reads in 168.078 CPU sec, 5.357 real sec
[M::mem_process_seqs] Processed 674046 reads in 90.838 CPU sec, 2.880 real sec
sorting block 60, #reads = 1079596 mem = 536879274 peak = 1099313152
[M::process] read 673778 sequences (100000232 bp) in 2.107 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285942, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (253.73, 128.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673614 sequences (100000059 bp) in 1.490 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285781, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (254.72, 129.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285488, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.59, 131.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 6, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857)
[M::mem_pestat] mean and std.dev: (219.75, 314.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2475)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673952 reads in 240.273 CPU sec, 7.676 real sec
[M::mem_process_seqs] Processed 673914 reads in 166.537 CPU sec, 5.334 real sec
[M::mem_process_seqs] Processed 673778 reads in 172.959 CPU sec, 5.558 real sec
sorting block 61, #reads = 1080030 mem = 536880600 peak = 1099313152
sorting block 62, #reads = 1079833 mem = 536880876 peak = 1112145920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285724, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (257.61, 131.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673500 sequences (100000127 bp) in 2.385 CPU sec, 2.651 real sec...
[M::mem_process_seqs] Processed 673614 reads in 87.112 CPU sec, 2.841 real sec
sorting block 63, #reads = 1080021 mem = 536882038 peak = 1112145920
[M::process] read 673446 sequences (100000056 bp) in 1.893 CPU sec, 1.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285034, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 191, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (263.44, 137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 899)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673400 sequences (100000254 bp) in 1.480 CPU sec, 1.482 real sec...
[M::process] read 673374 sequences (100000140 bp) in 1.270 CPU sec, 1.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285227, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (264.73, 137.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673500 reads in 168.390 CPU sec, 5.309 real sec
[M::mem_process_seqs] Processed 673446 reads in 93.959 CPU sec, 2.976 real sec
sorting block 64, #reads = 1079862 mem = 536879917 peak = 1112145920
[M::process] read 673278 sequences (100000265 bp) in 1.998 CPU sec, 2.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285362, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (266.33, 138.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 147, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (114.23, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673216 sequences (100000296 bp) in 1.249 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284581, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (270.05, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284870, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (267.70, 139.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673400 reads in 251.064 CPU sec, 8.060 real sec
[M::mem_process_seqs] Processed 673278 reads in 176.307 CPU sec, 5.724 real sec
[M::mem_process_seqs] Processed 673374 reads in 182.926 CPU sec, 5.957 real sec
sorting block 65, #reads = 1079997 mem = 536879808 peak = 1112145920
[M::process] read 673172 sequences (100000142 bp) in 1.946 CPU sec, 2.144 real sec...
sorting block 66, #reads = 1080023 mem = 536878516 peak = 1112145920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284920, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (271.38, 143.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673116 sequences (100000221 bp) in 1.370 CPU sec, 1.418 real sec...
[M::process] read 673014 sequences (100000282 bp) in 1.160 CPU sec, 1.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285059, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.36, 146.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673216 reads in 174.433 CPU sec, 5.582 real sec
[M::mem_process_seqs] Processed 673172 reads in 95.148 CPU sec, 3.024 real sec
sorting block 67, #reads = 1079746 mem = 536879760 peak = 1112145920
[M::process] read 673018 sequences (100000007 bp) in 1.654 CPU sec, 1.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284453, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (274.04, 146.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 936)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 1398, 1398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3768)
[M::mem_pestat] mean and std.dev: (780.27, 614.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4953)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674226 sequences (100000212 bp) in 1.157 CPU sec, 1.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284875, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (278.95, 151.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (66, 87, 705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983)
[M::mem_pestat] mean and std.dev: (377.29, 500.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2622)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285881, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (276.46, 149.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 577, 6448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18956)
[M::mem_pestat] mean and std.dev: (2356.75, 3605.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 25210)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673116 reads in 243.891 CPU sec, 7.819 real sec
[M::mem_process_seqs] Processed 673014 reads in 166.452 CPU sec, 5.362 real sec
[M::mem_process_seqs] Processed 673018 reads in 173.248 CPU sec, 5.597 real sec
sorting block 68, #reads = 1079774 mem = 536878513 peak = 1112145920
sorting block 69, #reads = 1079267 mem = 536878986 peak = 1112145920
[M::process] read 673922 sequences (100000098 bp) in 1.682 CPU sec, 1.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285696, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 186, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (248.38, 123.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (18, 24, 181)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507)
[M::mem_pestat] mean and std.dev: (75.42, 76.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 673824 sequences (100000167 bp) in 1.573 CPU sec, 1.613 real sec...
[M::process] read 673864 sequences (100000029 bp) in 1.140 CPU sec, 1.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285693, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 187, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (251.35, 125.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 230, 1788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5208)
[M::mem_pestat] mean and std.dev: (876.50, 1204.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6918)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674226 reads in 167.760 CPU sec, 5.344 real sec
[M::mem_process_seqs] Processed 673922 reads in 90.503 CPU sec, 2.872 real sec
sorting block 70, #reads = 1079841 mem = 536878150 peak = 1112145920
[M::process] read 673764 sequences (100000106 bp) in 1.733 CPU sec, 1.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285364, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (253.77, 127.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673596 sequences (100000111 bp) in 1.100 CPU sec, 1.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284970, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 189, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (257.82, 131.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285471, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 188, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (255.82, 129.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673824 reads in 245.318 CPU sec, 7.861 real sec
[M::mem_process_seqs] Processed 673764 reads in 169.160 CPU sec, 5.449 real sec
[M::mem_process_seqs] Processed 673864 reads in 175.454 CPU sec, 5.666 real sec
sorting block 71, #reads = 1080118 mem = 536881703 peak = 1112145920
sorting block 72, #reads = 1079811 mem = 536880648 peak = 1112145920
[M::process] read 673436 sequences (100000022 bp) in 2.269 CPU sec, 2.462 real sec...
[M::process] read 673328 sequences (100000018 bp) in 1.330 CPU sec, 1.335 real sec...
[M::process] read 673272 sequences (100000033 bp) in 1.120 CPU sec, 1.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284927, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.35, 138.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673436 reads in 92.728 CPU sec, 2.930 real sec
sorting block 73, #reads = 1079915 mem = 536878502 peak = 1112145920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284633, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 190, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (260.99, 134.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673206 sequences (100000075 bp) in 1.544 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284602, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 192, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (266.14, 138.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673596 reads in 266.933 CPU sec, 8.497 real sec
[M::mem_process_seqs] Processed 673328 reads in 104.993 CPU sec, 3.356 real sec
sorting block 74, #reads = 1080105 mem = 536881089 peak = 1112145920
[M::process] read 673116 sequences (100000281 bp) in 1.944 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284549, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 193, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (269.76, 141.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673170 sequences (100000059 bp) in 1.160 CPU sec, 1.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284420, 15, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 194, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (270.08, 141.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 916)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (11, 24, 106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296)
[M::mem_pestat] mean and std.dev: (41.07, 43.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 391)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284028, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 195, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (272.07, 143.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 370, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954)
[M::mem_pestat] mean and std.dev: (291.08, 301.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2597)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 673272 reads in 256.964 CPU sec, 8.228 real sec
[M::mem_process_seqs] Processed 673206 reads in 177.247 CPU sec, 5.709 real sec
[M::mem_process_seqs] Processed 673116 reads in 184.138 CPU sec, 5.949 real sec
sorting block 75, #reads = 1079529 mem = 536876894 peak = 1112145920
[M::process] read 673066 sequences (100000084 bp) in 1.940 CPU sec, 2.247 real sec...
sorting block 76, #reads = 1079373 mem = 536880664 peak = 1112145920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284365, 11, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 196, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (274.51, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (22, 25, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (174.91, 254.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672998 sequences (100000282 bp) in 1.353 CPU sec, 1.392 real sec...
[M::process] read 672952 sequences (100000103 bp) in 1.080 CPU sec, 1.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284210, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 197, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (276.54, 146.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673170 reads in 179.362 CPU sec, 5.736 real sec
[M::mem_process_seqs] Processed 673066 reads in 98.466 CPU sec, 3.132 real sec
sorting block 77, #reads = 1079687 mem = 536879232 peak = 1112145920
[M::process] read 372902 sequences (55407094 bp) in 1.192 CPU sec, 1.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284379, 15, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (279.44, 149.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (25, 86, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405)
[M::mem_pestat] mean and std.dev: (164.36, 179.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1865)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 157788, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (279.48, 151.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284568, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 198, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (281.30, 152.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 73, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (107.56, 139.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 672998 reads in 214.294 CPU sec, 6.911 real sec
[M::mem_process_seqs] Processed 372902 reads in 133.063 CPU sec, 4.302 real sec
[M::mem_process_seqs] Processed 672952 reads in 139.552 CPU sec, 4.526 real sec
sorting block 78, #reads = 1079347 mem = 536877168 peak = 1112145920
sorting block 79, #reads = 1079261 mem = 536878744 peak = 1153069056
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 85894152
overall: 69875478528 mem 13024.959 user 498.328 sys 697.986 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:20260603_LH00166_0309_A23WCJNLT4.T18-D0003255.L007\tPU:L007\tSM:T18-D0003255\tLB:T18-D0003255\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.T18-D0003255_R1.fastq.gz 0003.T18-D0003255_R2.fastq.gz
[main] Real time: 698.021 sec; CPU: 13523.290 sec
sorting block 80, #reads = 128915 mem = 64045311 peak = 1153069056
uniq header count 1
merging 81 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3305303685
output reads: 86520245
bam_mem_sort: 81 calls 40.758 user 0.828 sys 41.777 real
bam_write: 81 calls 16.530 user 0.852 sys 17.234 real
execute: 1 calls 17.210 user 0.627 sys 716.880 real
merge_files: 1 calls 17.178 user 0.615 sys 18.781 real
parse_chunk: 1282 calls 52.263 user 12.798 sys 66.243 real
read_chunk: 3451 calls 24.527 user 22.478 sys 392.785 real
sort_block: 1 calls 17.193 user 21.999 sys 381.478 real
write_chunk: 2167 calls 16.831 user 0.422 sys 17.743 real
overall: 2569236480 mem 753.342 user 46.943 sys 716.902 real
ls: cannot access '*.cram': No such file or directory